中国林州丙型肝炎病毒NS3、NS5A和NS5B抑制剂耐药相关替代物检测新方法的开发和实施

IF 2.2 4区医学 Q3 BIOCHEMICAL RESEARCH METHODS

Journal of virological methods Pub Date : 2024-12-16 DOI:10.1016/j.jviromet.2024.115102

Cui Zhang , Yugang Nie , Jian Li , Xiaoyu Ji , Mengjie Han , Rong Qin , Yuqiu Liu , Wenge Xing , Maofeng Qiu , Ning Li , Zhongfu Liu

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引用次数: 0

摘要

背景：丙型肝炎病毒（HCV）耐药相关替代药物（ras）对直接作用抗病毒药物（DAAs）治疗HCV有显著影响。然而，在中国大陆进行的研究有限，并且没有检测ras的标准化方法。目的：在中国林州市HCV rna阳性患者中建立并应用一种检测HCV RASs的新方法。研究设计：共收集103份HCV rna阳性血清标本和流行病学问卷。建立了一种检测包含NS3 ~ NS5B区的HCV RASs的PCR方法。结果：人口统计学分析显示，女性居多（66/103,64.1%），平均年龄70岁。基因型分别为1b (GT1b)（17/103, 16.5%）和GT2a（86/103, 83.5%）。GT1b组RASs患病率（17/ 17,100%）高于GT2a组（7/ 86,8%）。在GT1b中，NS5B区RASs发生率（17/ 17,100%）高于NS3区（14/ 17,82%）和NS5A区（10/ 17,59%）。C316N（100%）最为常见，其次是S122G（71%）和R30Q（35%）。结论：我们介绍了一种检测HCV RASs的创新方法，并提供了中国林州市HCV RASs的丰富信息。研究结果支持谨慎选择治疗方案，可能改善患者的预后。

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Development and implementation of a novel method for detecting hepatitis C virus resistance-associated substitutions to NS3, NS5A, and NS5B inhibitors in Linzhou, China

Background

Hepatitis C virus (HCV) resistance-associated substitutions (RASs) have a significant impact on the treatment of HCV with direct-acting antivirals (DAAs). However, limited research has been conducted, and no standardized methods for detecting RASs in mainland China.

Objectives

To develop and apply a novel method for detecting HCV RASs in HCV RNA-positive patients in Linzhou, China.

Study design

In total, 103 HCV RNA-positive serum specimens and epidemiological questionnaires were collected. A PCR method for detecting HCV RASs encompassing the NS3 to NS5B region was developed.

Results

Demographic analysis revealed a predominance of females (66/103, 64.1 %), with an average age of 70 years. Genotype 1b (GT1b) (17/103, 16.5 %) and GT2a (86/103, 83.5 %) were identified. The prevalence of RASs was higher (17/17, 100 %) in GT1b than in GT2a (7/86, 8 %). In GT1b, a higher frequency of RASs was observed in the NS5B region (17/17, 100 %) than in the NS3 (14/17, 82 %) and NS5A (10/17, 59 %) regions. C316N was the most prevalent, followed by S122G (71 %) and R30Q (35 %).

Conclusions

We introduced an innovative approach for the detection of HCV RASs and provided a wealth of information on HCV RASs in Linzhou, China. The findings support the cautious selection of treatment regimens, potentially improving patient outcomes.

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来源期刊

Journal of virological methods 医学-病毒学

CiteScore

5.80

自引率

0.00%

发文量

209

审稿时长

41 days

期刊介绍： The Journal of Virological Methods focuses on original, high quality research papers that describe novel and comprehensively tested methods which enhance human, animal, plant, bacterial or environmental virology and prions research and discovery. The methods may include, but not limited to, the study of: Viral components and morphology- Virus isolation, propagation and development of viral vectors- Viral pathogenesis, oncogenesis, vaccines and antivirals- Virus replication, host-pathogen interactions and responses- Virus transmission, prevention, control and treatment- Viral metagenomics and virome- Virus ecology, adaption and evolution- Applied virology such as nanotechnology- Viral diagnosis with novelty and comprehensive evaluation. We seek articles, systematic reviews, meta-analyses and laboratory protocols that include comprehensive technical details with statistical confirmations that provide validations against current best practice, international standards or quality assurance programs and which advance knowledge in virology leading to improved medical, veterinary or agricultural practices and management.