Journal of veterinary medicine. B, Infectious diseases and veterinary public health最新文献_第6页

Comparison of the antibody response in adult cattle against different epitopes of Brucella abortus lipopolysaccharide. 成年牛对不同产布鲁氏菌脂多糖表位抗体反应的比较。

Journal of veterinary medicine. B, Infectious diseases and veterinary public health Pub Date : 2001-10-27 DOI: 10.1046/J.1439-0450.2001.00476.X

N. Rojas, O. Zamora, J. Cascante, D. Garita, E. Moreno

{"title":"Comparison of the antibody response in adult cattle against different epitopes of Brucella abortus lipopolysaccharide.","authors":"N. Rojas, O. Zamora, J. Cascante, D. Garita, E. Moreno","doi":"10.1046/J.1439-0450.2001.00476.X","DOIUrl":"https://doi.org/10.1046/J.1439-0450.2001.00476.X","url":null,"abstract":"The comparison of serological responses in a sample of adult, vaccinated and held-infected bovines with Brucella abortus is reported. Indirect enzyme immunoassav (EIA) titration curves and Western blotting tests for smooth-type lipopolysaccharide (S-LPS), rough-type LPS (R-LPS) and lipid A were performed. In the initial screening of sera, an overall prevalence of 20.5% was found, which corresponds to a country with a high incidence of brucellosis. End-point EIA titres against LPS antigens from vaccinated and field-infected cows were not significantly different. However, the absorbance values in the titration curves were significantly higher for S-LPS as compared with the other antigens. A high correlation coefficient (r = 0.933) was obtained when the titres to R-LPS versus lipid A were compared. Western blotting reactions of vaccinated and held-infected animals were indistinguishable. S-LPS, R-LPS and lipid A epitopes were recognized in a heterogeneous manner. In general, the number of bovines that reacted against LPS was higher in the field-infected group, with a stronger binding to S-LPS. Based on our observations, the vaccinated and field-infected bovines are capable of producing similar antibody responses to the Brucella main outer surface antigen, LPS. It should be emphasized that the humoral response of cattle to Brucella LPS contains significant amounts of antibodies to other antigenic moieties of this important surface molecule, which may contribute to the immunity to brucellosis.","PeriodicalId":17659,"journal":{"name":"Journal of veterinary medicine. B, Infectious diseases and veterinary public health","volume":"53 1","pages":"623-9"},"PeriodicalIF":0.0,"publicationDate":"2001-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87609344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Why is anti-microbial resistance a veterinary problem as well? 为什么抗微生物药物耐药性也是一个兽医问题?

Journal of veterinary medicine. B, Infectious diseases and veterinary public health Pub Date : 2001-10-27 DOI: 10.1046/J.1439-0450.2001.00475.X

E. Mateu, M. Martín

引用次数: 55

Antibody-dependent killing of virus-infected targets by NK-like cells in bovine blood. 牛血液中nk样细胞对病毒感染目标的抗体依赖性杀伤。

Journal of veterinary medicine. B, Infectious diseases and veterinary public health Pub Date : 2001-10-27 DOI: 10.1046/J.1439-0450.2001.00479.X

H. Bradford, B. Adair, J. C. Foster

引用次数: 7

Pig paramyxovirus of the blue eye disease binding to a 116 kDa glycoprotein expressed in pig neuronal membranes. 猪蓝眼病副粘病毒与猪神经元膜表达的116 kDa糖蛋白结合。

Journal of veterinary medicine. B, Infectious diseases and veterinary public health Pub Date : 2001-09-15 DOI: 10.1046/J.1439-0450.2001.00462.X

M. L. Mendoza-Magaña, M. Ramírez-Herrera, J. M. Dueñas-Jiménez, S. Dueñas-Jiménez

{"title":"Pig paramyxovirus of the blue eye disease binding to a 116 kDa glycoprotein expressed in pig neuronal membranes.","authors":"M. L. Mendoza-Magaña, M. Ramírez-Herrera, J. M. Dueñas-Jiménez, S. Dueñas-Jiménez","doi":"10.1046/J.1439-0450.2001.00462.X","DOIUrl":"https://doi.org/10.1046/J.1439-0450.2001.00462.X","url":null,"abstract":"UNLABELLED\u0000Pig paramyxovirus of the blue eye disease (PPBED) is a novel member of the paramyxoviridac family which infects pigs. In neonatal pigs it causes neurological damage, whereas in adult pigs it affects the reproductive function. As PPBED damages the new-born pig central nervous system (CNS), it is important to study whether PPBED binds to the membrane proteins of all brain tissue, or selectively binds to neuronal tissue of the brain stem, olfactory bulb, hippocampus, cerebellum, frontal, temporal and parietal brain cortex. It is also important to establish whether it also infects neurones obtained from new-born, 60-day-old and adult pigs, and the role of carbohydrate residues in virus binding. The effect on virus binding of polyclonal antibodies against viral envelope proteins was also studied. Binding studies were performed using dot blot and virus overlay protein binding assays. PPBED was able to bind to membrane proteins from all brain regions, particularly to a protein band of approximately 116 kDa. Neuraminidase treatment of neuronal membrane proteins decreased virus binding; subsequent treatments with beta-galactosidase and manosidase did not increase virus binding inhibition. N-glycosidase F and trypsin also decreased virus binding, but not the O-glycanase. Antibodies against viral haemagglutinin-neuraminidase blocked virus binding more efficiently than antibodies against viral fusion protein.\u0000\u0000\u0000IN CONCLUSION\u0000(1) PPBFD is able to bind to pig neurones of all brain regions studied and at all ages analysed; (2) a 116 kDa membrane protein containing sialic acid residues with an N-linked oligosaccharide chain was specifically recognized; (3) PPBED haemagglutinin-neuraminidase protein seems to play a central role in neural receptor recognition.","PeriodicalId":17659,"journal":{"name":"Journal of veterinary medicine. B, Infectious diseases and veterinary public health","volume":"44 1","pages":"489-99"},"PeriodicalIF":0.0,"publicationDate":"2001-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73816616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Sows intramammarily inoculated with Escherichia coli influence of time of infection, hormone concentrations and leucocyte numbers on development of disease. 母猪乳内接种大肠杆菌感染时间、激素浓度和白细胞数量对疾病发展的影响。

Journal of veterinary medicine. B, Infectious diseases and veterinary public health Pub Date : 2001-09-15 DOI: 10.1046/J.1439-0450.2001.00469.X

Ulf Magnusson, A. Mörner, A. Persson, E. Karlstam, S. Sternberg, H. Kindahl

{"title":"Sows intramammarily inoculated with Escherichia coli influence of time of infection, hormone concentrations and leucocyte numbers on development of disease.","authors":"Ulf Magnusson, A. Mörner, A. Persson, E. Karlstam, S. Sternberg, H. Kindahl","doi":"10.1046/J.1439-0450.2001.00469.X","DOIUrl":"https://doi.org/10.1046/J.1439-0450.2001.00469.X","url":null,"abstract":"The aim of this study was to identify factors that influence the development of disease in sows inoculated with Escherichia coli in the mammary gland. Ten cross-bred primiparous sows were intramammarily inoculated with living E. coli bacteria at different time points before parturition: seven sows within 48 h before parturition and three sows approximately 96 h before parturition. Before and after inoculation, blood samples and mammary gland biopsy specimens were collected and clinical observations were made. All seven sows inoculated close to parturition developed a rectal temperature of >39.5 degrees C during the first 48 h post-partum and two of them also showed other signs of clinical disease. In the sows inoculated 4 days before parturition, the rectal temperature never exceeded 39.5 degrees C during the first 48 h post-partum and none of them showed any other sign of clinical discase. There was a tendency (P < 0.1) that histological signs of mastitis were more frequent in the sows inoculated close to parturition. There were no overall differences between the two groups of sows in plasma concentrations of cortisol, oestradiol-17beta and 15-ketodihydro-PGF2alpha before inoculation. Before inoculation, the number of neutrophils in the blood was overall higher (P < 0.05) in the group of sows that were inoculated close to parturition. In comparison, the number of lymphocytes before inoculation had a tendency (P < 0.1) to be lower in that group. The data suggest that the time of infection of the mammary gland relative to parturition and the number of circulating neutrophils at the time of infection influence the development of chinical coliform mastitis in the sow.","PeriodicalId":17659,"journal":{"name":"Journal of veterinary medicine. B, Infectious diseases and veterinary public health","volume":"20 1","pages":"501-12"},"PeriodicalIF":0.0,"publicationDate":"2001-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75442387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Studies on the prevalence of Riemerella anatipestifer in the upper respiratory tract of clinically healthy ducklings and characterization of untypable strains. 临床健康雏鸭上呼吸道鸭疫里默氏菌流行病学及不典型菌株特征的研究。

Journal of veterinary medicine. B, Infectious diseases and veterinary public health Pub Date : 2001-09-15 DOI: 10.1046/J.1439-0450.2001.00471.X

M. Ryll, Henrik Christensen, Magne Bisgaard, Jens Peter Christensen, K.‐H. Hinz, B. Köhler

引用次数: 38

Systemic aspergillosis in an oiled magallanic penguin (Spheniscus magellanicus). 涂油麦哲伦企鹅(麦哲伦企鹅)的全身曲霉病。

Journal of veterinary medicine. B, Infectious diseases and veterinary public health Pub Date : 2001-09-15 DOI: 10.1046/J.1439-0450.2001.00456.X

Librado Carrasco, J. S. Lima, D. Halfen, F. Salguero, P. Sánchez-Cordón, G. Becker

引用次数: 55

Endometritis in she donkeys in Egypt. 埃及驴的子宫内膜炎。

Journal of veterinary medicine. B, Infectious diseases and veterinary public health Pub Date : 2001-09-15 DOI: 10.1046/J.1439-0450.2001.00457.X

S. Sokkar, M. Hamouda, S. M. El-Rahman

引用次数: 8

Antimicrobial susceptibility of Pasteurella multocida isolated from cattle and pigs. 牛和猪多杀性巴氏杆菌的药敏分析。

Journal of veterinary medicine. B, Infectious diseases and veterinary public health Pub Date : 2001-09-15 DOI: 10.1046/J.1439-0450.2001.00468.X

H. Yoshimura, M. Ishimaru, Y. S. Endoh, A. Kojima

引用次数: 46

Effect of subcutaneous injection of ginseng on cows with subclinical Staphylococcus aureus mastitis. 人参皮下注射对奶牛亚临床金黄色葡萄球菌性乳腺炎的影响。

Journal of veterinary medicine. B, Infectious diseases and veterinary public health Pub Date : 2001-09-15 DOI: 10.1046/J.1439-0450.2001.00470.X

Songhua Hu, C. Concha, A. Johannisson, G. Meglia, K. P. Waller

{"title":"Effect of subcutaneous injection of ginseng on cows with subclinical Staphylococcus aureus mastitis.","authors":"Songhua Hu, C. Concha, A. Johannisson, G. Meglia, K. P. Waller","doi":"10.1046/J.1439-0450.2001.00470.X","DOIUrl":"https://doi.org/10.1046/J.1439-0450.2001.00470.X","url":null,"abstract":"Cows with subclinical mastitis caused by Staphylococcus aureus were subjected to subcutaneous injections with either an extract from the root of Panax ginseng CA Meyer at a dose of 8 mg/kg body weight per day for 6 days, or with saline as a control. The injection areas were checked for adverse reactions. The daily milk production was measured before and after treatment. Blood was collected for total and differential leucocyte counts, identification of lymphocyte subpopulations using flow cytometry, lymphocyte proliferation test, and neutrophil phagocytosis and oxidative burst assay. Quarter milk samples were collected for bacteriological analysis and somatic cell counts (SCC). After the end of treatment, the numbers of S. aureus-infected quarters and milk SCC tended to decrease in ginseng-treated cows. Phagocytosis and oxidative burst activity of blood neutrophils were significantly increased 1 week after ginseng treatment, but the proliferative response of blood lymphocytes did not change significantly. The number of monocytes in ginseng-injected cows was significantly higher 1 week post-treatment than pre-treatment, and the number of lymphocytes was significantly higher than pre-infusion at 2 and 3 weeks after ginseng treatment. Similar changes were not observed in the control group. The present findings indicate that ginseng treatment can activate the innate immunity of cows and may contribute to the cow's recovery from mastitis. It is therefore suggested that ginseng has a potential as a stimulator of the immune system of dairy cows.","PeriodicalId":17659,"journal":{"name":"Journal of veterinary medicine. B, Infectious diseases and veterinary public health","volume":"95 1","pages":"519-28"},"PeriodicalIF":0.0,"publicationDate":"2001-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84949868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 51