C. Hamers, E. Di Valentin, C. Lecomte, M. Lambot, E. Joris, B. Genicot, P. Pastoret
{"title":"Virus neutralizing antibodies against a panel of 18 BVDV isolates in calves vaccinated with Rispoval RS-BVD.","authors":"C. Hamers, E. Di Valentin, C. Lecomte, M. Lambot, E. Joris, B. Genicot, P. Pastoret","doi":"10.1046/J.1439-0450.2000.00405.X","DOIUrl":"https://doi.org/10.1046/J.1439-0450.2000.00405.X","url":null,"abstract":"Seven of nine colostrum-deprived calves, free from infection with bovine virus diarrhoea virus (BVDV), were vaccinated with Rispoval RS-BVD on two occasions, 21 days apart, while the other two were kept as BVDV infection controls. The virus neutralizing (VN) serum antibodies induced by vaccination were tested for their ability to neutralize 18 European BVDV isolates, including laboratory reference strains and recent field isolates, both cytopathic and non-cytopathic biotypes as well as genotypes I and II. The strains were isolated in Belgium, France, Germany and the United Kingdom. While there were large variations in the vaccine-induced VN titres of the individual calves against all the strains, e.g. the titres against Osloss NCP, the European reference strain ranged from 1.7 to 6.7 (1:log2), serum from each animal was capable of neutralizing between nine and all 18 of the strains tested. Nevertheless, from the results of this study, it can be concluded that in colostrum-deprived BVDV seronegative calves, Rispoval RS-BVD can stimulate the production of VN antibodies capable of neutralizing a wide range of antigenically diverse European isolates of BVDV, including genotypes I and II.","PeriodicalId":17659,"journal":{"name":"Journal of veterinary medicine. B, Infectious diseases and veterinary public health","volume":"13 1","pages":"721-6"},"PeriodicalIF":0.0,"publicationDate":"2000-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89120042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Ferrari, A. Brack, Maria Grazia Romanelli, Tc Mettenleiter, Attilio Corradi, N. D. Mas, Mn Losio, R. Silini, C. Pinoni, A. Pratelli
{"title":"A study of the ability of a TK-negative and gI/gE-negative pseudorabies virus (PRV) mutant inoculated by different routes to protect pigs against PRV infection.","authors":"M. Ferrari, A. Brack, Maria Grazia Romanelli, Tc Mettenleiter, Attilio Corradi, N. D. Mas, Mn Losio, R. Silini, C. Pinoni, A. Pratelli","doi":"10.1046/J.1439-0450.2000.00407.X","DOIUrl":"https://doi.org/10.1046/J.1439-0450.2000.00407.X","url":null,"abstract":"The capacity of a TK-negative (TK-) and gI/gE-negative (gI/gE-) pseudorabies virus (PRV) mutant to protect pigs against Aujeszky's disease carried out by experimental infection with a virulent PRV strain, was tested. There were three groups, each of four susceptible pigs which were inoculated twice by two different schedules. Group 1 received the modified virus by the intradermal (first inoculation)-intramuscular (second inoculation) routes; group 2 was treated by the intranasal (first inoculation)-intramuscular (second inoculation) routes. The third group was left untreated as the control. All of the pigs were challenged intranasally with a virulent PRV strain and they were subsequently injected with dexamethasone. Two pigs in each group were necropsied on days 5 and 15 after dexamethasone inoculation. The challenge exposure resulted in mild clinical signs, increase in growth and a shorter period of virus shedding in vaccinated pigs, whereas the control group showed severe signs of Aujeszky's disease. No difference in the titre of the virulent virus which was excreted by pigs of all three groups, was observed and all animals seroconverted. Both the mutant strain and the wild-type virus established a latent infection although only the latter was reactivated and shed. Slight lesions were observed in target tissues of the vaccinated animals and no significant differences were detected between the two inoculation schedules.","PeriodicalId":17659,"journal":{"name":"Journal of veterinary medicine. B, Infectious diseases and veterinary public health","volume":"153 1","pages":"753-62"},"PeriodicalIF":0.0,"publicationDate":"2000-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76152264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E Rautiainen, A M Virtala, P Wallgren, H Saloniemi
{"title":"Varying effects of infections with Mycoplasma hyopneumoniae on the weight gain recorded in three different multisource fattening pig herds.","authors":"E Rautiainen, A M Virtala, P Wallgren, H Saloniemi","doi":"10.1046/j.1439-0450.2000.00370.x","DOIUrl":"https://doi.org/10.1046/j.1439-0450.2000.00370.x","url":null,"abstract":"<p><p>Pigs in three specialized fattening herds were studied with respect to the effect of infection with Mycoplasma hyopneumoniae on weight gain. Individual pigs were weighed four times at 4-week intervals during the fattening period and their daily weight gain over the rearing period was calculated. A blood sample was collected on each weighing occasion and analysed for the presence of antibodies to M. hyopneumoniae. The lungs of the principals were inspected at slaughter and the extent of pneumonic lesions was registered by a specially developed technique that has been proven to warrant a high degree of repeatability. No serum antibodies to M. hyopneumoniae were detected in one of the herds, and no pneumonic lesions were recorded at slaughter in that herd. In the other two herds, the prevalence of pigs with serum antibodies to M. hyopneumoniae increased from 6 to 54% and from 31 to 81%, respectively, during the fattening period. The prevalence of pneumonic lesions at slaughter in these herds was higher the later the pigs seroconverted. On the other hand, the extension of the lung lesions tended to be higher among pigs that seroconverted early during the rearing period. Infections with M. hyopneumoniae acquired early during the rearing, presumably strengthened by secondary infections and environmental errors, was found to decrease the daily weight gain of the pigs. However, even non-complicated M. hyopneumoniae infections acquired late in the fattening period were associated with reduced daily weight gain. That growth reduction was estimated to be at least 60 g (about 6%) after adjusting for herd, pen, initial weight and sex.</p>","PeriodicalId":17659,"journal":{"name":"Journal of veterinary medicine. B, Infectious diseases and veterinary public health","volume":"47 6","pages":"461-9"},"PeriodicalIF":0.0,"publicationDate":"2000-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1046/j.1439-0450.2000.00370.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21847692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The sensitivities of various erythrocytes in a haemagglutination assay for the detection of psittacine beak and feather disease virus.","authors":"N Sanada, Y Sanada","doi":"10.1046/j.1439-0450.2000.00360.x","DOIUrl":"https://doi.org/10.1046/j.1439-0450.2000.00360.x","url":null,"abstract":"<p><p>The erythrocytes of various species were tested in psittacine beak and feather disease (PBFD) virus haemagglutination (HA) and haemagglutination inhibition assays to determine which are suitable for use in these assays. HA activity was observed for erythrocytes of the salmon-crested cockatoo, the sulphur-crested cockatoo, the umbrella cockatoo, the goffin's cockatoo and the cockatiel, with differences amongst individuals within species, but not for erythrocytes of humans, the pig, the guinea pig, the chicken, the goose, the rose-ringed parakeet or the budgerigar. Anti-PBFD virus rabbit sera inhibited the virus-induced agglutination of erythrocytes, confirming the specificity of HA activity. This suggests that selection of suitable psittacine species as well as suitable individuals within a species is necessary when obtaining erythrocytes for the PBFD virus HA assay.</p>","PeriodicalId":17659,"journal":{"name":"Journal of veterinary medicine. B, Infectious diseases and veterinary public health","volume":"47 6","pages":"441-3"},"PeriodicalIF":0.0,"publicationDate":"2000-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1046/j.1439-0450.2000.00360.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21847689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I Luque, C Tarradas, L Carrasco, E Torroella, C Artigas, A Perea
{"title":"Effectiveness of doxycycline in the prevention of an experimental infection with Actinobacillus pleuropneumoniae in pigs.","authors":"I Luque, C Tarradas, L Carrasco, E Torroella, C Artigas, A Perea","doi":"10.1046/j.1439-0450.2000.00368.x","DOIUrl":"https://doi.org/10.1046/j.1439-0450.2000.00368.x","url":null,"abstract":"<p><p>The effectiveness of medication with doxycycline in feed in the control of pleuropneumonia in pigs was tested using an Actinobacillus pleuropneumoniae serotype 1 aerosol challenge model. Two groups of 10 animals were used for the challenge, a 'medicated group' and an 'unmedicated group'. A third group of four animals was used as a 'control group'. Pigs from the medicated group were provided with feed containing 250 p.p.m. doxycycline (HIPRAMIX/DOXI) for 8 consecutive days and were challenged on the fifth day of treatment. No clinical signs were observed in pigs from the 'control group'. Four animals from the 'unmedicated group' died within the first 48 h after challenge with clinical and lesional evidence of an acute form of pleuropneumonia. Clinical signs of animals surviving the first 48 h were progressively less severe and showed lesions similar to those described for subacute-chronic forms of the disease. However, only one animal from the 'medicated group' showed clinical signs of a chronic form of pleuropneumonia. Reisolation of A. pleuropneumoniae was more evident from lung tissues of animals fed the doxycycline-free feed (70%), coinciding with the presence of both acute and subacute lesions. However, the micro-organism could be reisolated from only one animal which belonged to the 'medicated group'. It is concluded that the treatment of pigs with 250 p.p.m. doxycycline (HIPRAMIX/DOXI) prevents disease caused by A. pleuropneumoniae.</p>","PeriodicalId":17659,"journal":{"name":"Journal of veterinary medicine. B, Infectious diseases and veterinary public health","volume":"47 6","pages":"445-51"},"PeriodicalIF":0.0,"publicationDate":"2000-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1046/j.1439-0450.2000.00368.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21847691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M Busch, R Thoma, I Schiller, L Corboz, A Pospischil
{"title":"Occurrence of chlamydiae in the genital tracts of sows at slaughter and their possible significance for reproductive failure.","authors":"M Busch, R Thoma, I Schiller, L Corboz, A Pospischil","doi":"10.1046/j.1439-0450.2000.00415.x","DOIUrl":"https://doi.org/10.1046/j.1439-0450.2000.00415.x","url":null,"abstract":"<p><p>The aim of this study was to investigate further the role of chlamydiae as pathogens in the genital tracts of sows at slaughter. Genital tracts of 101 randomly selected sows were collected and specimens of genital tract localizations were systematically examined for chlamydiae using immunohistochemistry and PCR. In the genital tracts of 10 sows, Chlamydia psittaci DNA was detected by PCR, and was further typed as 'serotype 1' in nine cases and as avian strain 6 BC in one animal. However, all specimens examined by immunohistochemistry were negative for chlamydiae. Pooled samples of scalding tank water were additionally investigated for 95 animals. Of these samples, 63.2% contained chlamydial DNA, mostly C. trachomatis, and in one sample C. psittaci 'serotype 1'. Although in most cases contamination through influx of faecally contaminated scalding water is a possible reason for the positive PCR results in the genital tract, latent infection cannot be excluded. In conclusion, the results obtained suggest that chlamydiae are of no or only minor importance in the examined group of Swiss breeding sows. Nevertheless, the role and significance of chlamydiae as pathogens in porcine reproductive disorders remain unresolved and require further investigation.</p>","PeriodicalId":17659,"journal":{"name":"Journal of veterinary medicine. B, Infectious diseases and veterinary public health","volume":"47 6","pages":"471-80"},"PeriodicalIF":0.0,"publicationDate":"2000-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1046/j.1439-0450.2000.00415.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21847693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}