Journal of veterinary medicine. B, Infectious diseases and veterinary public health最新文献

Histopathological and immunohistochemical study of lambs experimentally infected with Fasciola hepatica and Schistosoma bovis. 羔羊肝片形吸虫和牛血吸虫实验感染的组织病理学和免疫组化研究。

Journal of veterinary medicine. B, Infectious diseases and veterinary public health Pub Date : 2008-07-07 DOI: 10.1111/j.1439-0450.2000.00410.x

M. C. Ferreras, M. García-Iglesias, M. Y. Manga-González, C. Pérez-Martínez, Y. Mizinska, V. Ramajo, M. Gonzalez-Lanza, A. Escudero, J. F. García-Marín

{"title":"Histopathological and immunohistochemical study of lambs experimentally infected with Fasciola hepatica and Schistosoma bovis.","authors":"M. C. Ferreras, M. García-Iglesias, M. Y. Manga-González, C. Pérez-Martínez, Y. Mizinska, V. Ramajo, M. Gonzalez-Lanza, A. Escudero, J. F. García-Marín","doi":"10.1111/j.1439-0450.2000.00410.x","DOIUrl":"https://doi.org/10.1111/j.1439-0450.2000.00410.x","url":null,"abstract":"The aim of this study was to investigate the cross-resistance between Fasciola hepatica and Schistosoma bovis in lambs assessing parasitologic, gross pathologic, histopathologic and immunohistochemical changes in liver and small intestine. Thirty Castellana breed lambs were divided into five comparable groups and exposed to F. hepatical S. bovis (group F/S), S. bovis/F. hepatica (group S/F), S. bovis (group S) or F. hepatica (group F) and six unexposed lambs were used as non-infected controls (group C). Primary patent infection with F. hepatica induced a lower number of schistosome eggs and a higher number of lymphocytes in intestinal and liver schistosome egg-induced granulomas in group F/S than in the groups S/F and S, liver damage being mainly attributed to F. hepatica. S. bovis infection followed by challenge with F. hepatica particularly increased the severity of the most significant liver alterations (cholangiohepatitis by F. hepatica and mesoendophlebitis by S. bovis) and F. hepatica seemed not to have an influence on established S. bovis infection. In addition, immunohistochemical results suggested that the predominant local immune response in both double-infected groups was different, being mainly a cell-mediated immune response in group F/S and a mucosal response in group S/F.","PeriodicalId":17659,"journal":{"name":"Journal of veterinary medicine. B, Infectious diseases and veterinary public health","volume":"5 1","pages":"763-73"},"PeriodicalIF":0.0,"publicationDate":"2008-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85363671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Comparative study of PCR as a direct assay and ELISA and AGID as indirect assays for the detection of bovine leukaemia virus. PCR与ELISA和AGID间接检测牛白血病病毒的比较研究。

Journal of veterinary medicine. B, Infectious diseases and veterinary public health Pub Date : 2008-07-07 DOI: 10.1111/j.1439-0450.2001.00424.x

D. Martin, A. Arjona, I. Soto, N. Barquero, M. Viana, E. Gómez-Lucia

引用次数: 22

Use of polymerase chain reaction to identify Brucella abortus strain RB51 among Brucella field isolates from cattle in Italy. 利用聚合酶链反应在意大利牛布鲁氏菌野外分离株中鉴定流产布鲁氏菌RB51。

Journal of veterinary medicine. B, Infectious diseases and veterinary public health Pub Date : 2008-07-07 DOI: 10.1111/j.1439-0450.2001.00432.x

R. Adone, F. Ciuchini, G. La Rosa, C. Marianelli, M. Muscillo

{"title":"Use of polymerase chain reaction to identify Brucella abortus strain RB51 among Brucella field isolates from cattle in Italy.","authors":"R. Adone, F. Ciuchini, G. La Rosa, C. Marianelli, M. Muscillo","doi":"10.1111/j.1439-0450.2001.00432.x","DOIUrl":"https://doi.org/10.1111/j.1439-0450.2001.00432.x","url":null,"abstract":"Brucella abortus strain RB51, a rough mutant of the B. abortus 2308 virulent strain, was recently approved in the United States as the official vaccine for brucellosis in cattle. Following recent evidence of unauthorized use of RB51 vaccine in Italy, where the use of vaccines for brucellosis is no longer allowed, the suitability of an RB51-specific polymerase chain reaction assay for identifying the RB51 strain among Brucella field isolates from cattle in Italy was investigated. The oligonucleotide primers used in this study, belonging to a six-primer cocktail for Brucella species previously described by other authors, allowed the amplification of a 364-base pair (bp) fragment specific for RB51 and its parent strain 2308, and a 498-bp product specific for B. abortus. In addition, unresolved bands ranging from 600 to 700 bp were observed from RB51 strain. Brucella abortus biovars 1, 2 and 4 have only one specific sensitive 498-bp band. The B. abortus biovars 3, 5 and 6 did not give any signal. The 498-bp product from a reference Brucella strain was sequenced and submitted to EMBL with the accession number AJ271969 while the 364-bp fragment from RB51 strain was submitted to EMBL database with accession number AJ271968. The sequence studies confirmed the specificity of the detected fragments. No amplification was obtained by testing DNA from strains antigenically related to Brucella, such as Yersinia enterocolitica O:9, Escherichia coli O:157, Salmonella urbana and Pasteurella multocida. The results of this study indicate that this technique, in combination with specific serological tests, could be a useful diagnostic method to verify the use of RB51 vaccine and can contribute to the creation of a databank of circulating strains.","PeriodicalId":17659,"journal":{"name":"Journal of veterinary medicine. B, Infectious diseases and veterinary public health","volume":"5 1","pages":"107-13"},"PeriodicalIF":0.0,"publicationDate":"2008-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91382627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Keratoconjunctivitis and periorbital papillomatosis associated with heavy periorbital infestation by the tail louse Haematopinus quadripertusus in heifers. 四百日咳血蜱尾虱对小母牛眼眶周围的严重侵害与角膜结膜炎和眼眶周围乳头状瘤病有关。

Journal of veterinary medicine. B, Infectious diseases and veterinary public health Pub Date : 2008-07-07 DOI: 10.1111/J.1439-0450.2001.00425.X

I. Yeruham, A. Hadani, S. Perl, D. Elad

引用次数: 2

Studies on Johne's disease in Egyptian cattle. 埃及牛约翰氏病的研究。

Journal of veterinary medicine. B, Infectious diseases and veterinary public health Pub Date : 2005-04-01 DOI: 10.1111/j.1439-0450.2005.00832.x

M Salem, A A Zeid, D Hassan, A El-Sayed, M Zschoeck

引用次数: 42

Relationship between rainfall and Neospora caninum-associated abortion in two dairy herds in a dry environment. 干旱环境下两个奶牛群中降雨与犬新孢子虫相关流产的关系。

Journal of veterinary medicine. B, Infectious diseases and veterinary public health Pub Date : 2005-04-01 DOI: 10.1111/j.1439-0450.2005.00831.x

F López-Gatius, I García-Ispierto, P Santolaria, J L Yániz, M López-Béjar, C Nogareda, S Almería

{"title":"Relationship between rainfall and Neospora caninum-associated abortion in two dairy herds in a dry environment.","authors":"F López-Gatius, I García-Ispierto, P Santolaria, J L Yániz, M López-Béjar, C Nogareda, S Almería","doi":"10.1111/j.1439-0450.2005.00831.x","DOIUrl":"https://doi.org/10.1111/j.1439-0450.2005.00831.x","url":null,"abstract":"The possible direct relationship between climate variations and abortion in Neospora caninum-infected cows has not been studied. The objective of this study was to determine whether climate changes could be a risk factor for abortion in N. caninum-infected cows, and was based on yearly serological screening for neosporosis and on the confirmation of N. caninum infection on aborted fetuses in two high-producing dairy herds with a mean 27% seroprevalence of N. caninum antibodies. The final population study was comprised of 357 pregnancies in seropositive animals. Logistic regression analysis indicated no significant effects of herd, N. caninum antibody titre, climate variables during the first and third trimesters of gestation, mean and maximum temperature-humidity index values during the second trimester of gestation, and previous abortion on the abortion rate. Based on the odds ratio, a 1-unit increase in lactation number yielded a 0.85-fold decrease in the abortion rate. The likelihood of abortion was 1.9 times (1/0.54) lower for pregnant cows inseminated with beef bull semen compared with Holstein-Friesian bull semen. The likelihood of abortion decreased significant and progressively by factors of 0.5, 0.41 and 0.3 for the respective classes 40-49, 30-39 and <30 rainfall mm during the second trimester of gestation (using the class >or=60 rainfall mm as reference). As a general conclusion, it seems that increased rainfall in a dry environment can compromise the success of gestation in N. caninum-infected cows. Attempts should therefore be made to reduce environment effects during the second trimester of gestation, a period in which the immune response of cows is diminished.","PeriodicalId":17659,"journal":{"name":"Journal of veterinary medicine. B, Infectious diseases and veterinary public health","volume":"52 3","pages":"147-52"},"PeriodicalIF":0.0,"publicationDate":"2005-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1439-0450.2005.00831.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25268094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 23

Identification and molecular characterization of Streptococcus equi subsp. zooepidemicus isolated from camels (Camelus dromedarius) and camel milk in Kenya and Somalia. 马链球菌亚种的鉴定与分子特性研究。从肯尼亚和索马里骆驼(单峰骆驼)和骆驼奶中分离的动物流行病。

Journal of veterinary medicine. B, Infectious diseases and veterinary public health Pub Date : 2005-04-01 DOI: 10.1111/j.1439-0450.2005.00828.x

M Younan, A T S Estoepangestie, M Cengiz, J Alber, A El-Sayed, C Lämmler

引用次数: 20

A novel subgroup among genotypes of equine arteritis virus: genetic comparison of 40 strains. 马动脉炎病毒基因型中的一个新亚群:40株的遗传比较。

Journal of veterinary medicine. B, Infectious diseases and veterinary public health Pub Date : 2005-04-01 DOI: 10.1111/j.1439-0450.2005.00833.x

A Hornyák, T Bakonyi, G Tekes, L Szeredi, M Rusvai

{"title":"A novel subgroup among genotypes of equine arteritis virus: genetic comparison of 40 strains.","authors":"A Hornyák, T Bakonyi, G Tekes, L Szeredi, M Rusvai","doi":"10.1111/j.1439-0450.2005.00833.x","DOIUrl":"https://doi.org/10.1111/j.1439-0450.2005.00833.x","url":null,"abstract":"The authors determined partial nucleic sequences of the variable regions of open-reading frame (ORF5) from 151 nucleotide to 668 nucleotide and deduced amino acid sequences of 518 nucleotide respectively of 20 equine arteritis virus (EAV) isolates. About 19 Hungarian and one Austrian EAV strains were subjected to sequence analysis, the further data of 20 EAV strains: six North American and 14 European were obtained from the GenBank. Comparative sequence analysis of the Hungarian EAV strains indicated that among the three variable regions the first has been affected mostly by point mutations. Genetic comparison of the Hungarian strains with other EAV isolates from western Europe and North America (including the Bucyrus reference strain) has been performed on the aforementioned genome region. Besides the already known genetic subgroups of EAV; phylogenetic analysis revealed a novel subgroup comprising mainly Hungarian strains. Compared with the Bucyrus virus, the overall sequence divergencies of the examined Hungarian strains ranged from 81.47 to 90.73% at nucleotide and from 84.88 to 91.86% at amino acid level. Epizootiological studies have shown that the significant part of the EAV strains having been existed in Hungary before and in 2000 belong to this unique cluster (II.D) which was not indicated in former phylogenetic studies. After 2000 new EAV strains emerged in Hungary, one of them causing abortions or neonatal death. The previously dominant 'Hungarian' EAV genotypes were replaced by these new strains belonging to North American and European subgroups (I.A, I.B, II.A, II.B). The anamnesis of these cases revealed connections with persistent virus shedder stallions, those were imported to the country after 2000 or have been infected abroad. One of these Hungarian stallions became the source of abortion storms in Hungarian studs.","PeriodicalId":17659,"journal":{"name":"Journal of veterinary medicine. B, Infectious diseases and veterinary public health","volume":"52 3","pages":"112-8"},"PeriodicalIF":0.0,"publicationDate":"2005-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1439-0450.2005.00833.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25094947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

Use of monoclonal antibodies in blocking ELISA detection of transmissible gastroenteritis virus in faeces of piglets. 应用单克隆抗体阻断ELISA检测仔猪粪便中传染性胃肠炎病毒。

Journal of veterinary medicine. B, Infectious diseases and veterinary public health Pub Date : 2005-04-01 DOI: 10.1111/j.1439-0450.2005.00829.x

L Rodák, B Smíd, Z Nevoránková, L Valícek, R Smítalová

引用次数: 15

Investigations into the basis of chloramphenicol and tetracycline resistance in Staphylococcus intermedius isolates from cases of pyoderma in dogs. 犬脓皮病中葡萄球菌对氯霉素和四环素耐药基础的调查。

Journal of veterinary medicine. B, Infectious diseases and veterinary public health Pub Date : 2005-04-01 DOI: 10.1111/j.1439-0450.2005.00836.x

T J Kim, Y R Na, J I Lee

{"title":"Investigations into the basis of chloramphenicol and tetracycline resistance in Staphylococcus intermedius isolates from cases of pyoderma in dogs.","authors":"T J Kim, Y R Na, J I Lee","doi":"10.1111/j.1439-0450.2005.00836.x","DOIUrl":"https://doi.org/10.1111/j.1439-0450.2005.00836.x","url":null,"abstract":"A total of 160 Staphylococcus intermedius isolates were recovered from cases of pyoderma in 2002 and were examined for susceptibility to 13 different antimicrobial agents. Ninety per cent (144) of the isolates were resistant to tetracycline, derivatives of which have been used until recently, and 18% (29) were resistant to chloramphenicol which was banned from use 13 years ago. The presence of genes encoding chloramphenicol acetyltransferase (CAT) and tetracycline resistance (tet); tet(K), (L), (M), and (O) were determined by PCR in the 29 chloramphenicol and tetracycline resistant isolates. Seventeen (59%) isolates contained the cat gene while 12 (41%) isolates did not carry the cat gene, implying there may be other genes for chloramphenicol resistance that were not detected by the primers (primer set 1) used in this study. The tet(M) gene was found in 28 (97%) of the resistant S. intermedius isolates, but none contained the tet(O) gene. All 29 isolates carried one or two tet genes; tet(K), (L), and (M), with four different distribution patterns. New PCR products, a 1.1 kb product using primer set 1 and a 0.2 kb product using primer set 2, were cloned and sequenced. A 904 bp fragment of S. aureus plamid pS194, including sequence from the streptomycin adenyltransferase gene (804 bp), was found inserted into the terminal region of the cat gene (GenBank accession no. AY604739), whilst the sequence of 0.2 kb was previously unpublished.","PeriodicalId":17659,"journal":{"name":"Journal of veterinary medicine. B, Infectious diseases and veterinary public health","volume":"52 3","pages":"119-24"},"PeriodicalIF":0.0,"publicationDate":"2005-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1439-0450.2005.00836.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25094948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 23