Journal of Veterinary Science最新文献

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Essential oil pharmaceuticals for killing ectoparasites on dogs. 杀灭狗体外寄生虫的精油药剂。
IF 1.8 3区 农林科学
Journal of Veterinary Science Pub Date : 2024-01-01 DOI: 10.4142/jvs.23189
Phacharaporn Tadee, Sunee Chansakaow, Pramote Tipduangta, Pakpoom Tadee, Pakasinee Khaodang, Kridda Chukiatsiri
{"title":"Essential oil pharmaceuticals for killing ectoparasites on dogs.","authors":"Phacharaporn Tadee, Sunee Chansakaow, Pramote Tipduangta, Pakpoom Tadee, Pakasinee Khaodang, Kridda Chukiatsiri","doi":"10.4142/jvs.23189","DOIUrl":"10.4142/jvs.23189","url":null,"abstract":"<p><strong>Background: </strong>External parasites, particularly ticks and fleas, are among the most common problems affecting dogs. Chemical medicines are commonly used to prevent and eliminate such external parasites, but their improper use can cause adverse reactions, and the toxins they contain may remain in the environment.</p><p><strong>Objectives: </strong>The objective of this study was to investigate the <i>in vitro</i> efficacy of <i>Zanthoxylum limonella</i>, citronella, clove, peppermint, and ginger essential oils against dog ticks and fleas and to test the sensitivity of dogs' skin to these essential oils.</p><p><strong>Methods: </strong>The five essential oils were tested for in vitro efficacy against ticks and fleas, and the two most effective essential oils were then tested on the dogs' skin.</p><p><strong>Results: </strong>The results revealed that these five essential oils at 16% concentrations effectively inhibited the spawning of female engorged ticks. In addition, all five essential oils had a strong ability to kill tick larvae at concentrations of 2% upward. Furthermore, 4% concentrations of the five essential oils quickly eliminated fleas, especially clove oil, which killed 100% of fleas within 1 h. A 50%, 90%, and 99% lethal concentration (LC<sub>50</sub>, LC<sub>90</sub>, and LC<sub>99</sub>) for the essential oils on tick larvae in 24 h were found to be low values. LC<sub>50</sub>, LC<sub>90</sub>, and LC<sub>99</sub> for the essential oils on flea in 1 h was lowest values. Clove oil at 16% concentration was the most satisfactory essential oil for application on dogs' skin, with a low percentage of adverse effects.</p><p><strong>Conclusions: </strong>This study confirmed the effectiveness of essential oils for practical use as tick and flea repellents and eliminators. Essential-oil-based pharmaceutical can replace chemical pesticides and provide benefits for both consumers and the environment.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"25 1","pages":"e5"},"PeriodicalIF":1.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10839170/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139692163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cervical spine reconstruction after total vertebrectomy using customized three-dimensional-printed implants in dogs. 在狗身上使用定制的三维打印植入物进行全脊椎切除术后的颈椎重建。
IF 1.8 3区 农林科学
Journal of Veterinary Science Pub Date : 2024-01-01 DOI: 10.4142/jvs.23241
Ji-Won Jeon, Kyu-Won Kang, Woo-Keyoung Kim, Sook Yang, Byung-Jae Kang
{"title":"Cervical spine reconstruction after total vertebrectomy using customized three-dimensional-printed implants in dogs.","authors":"Ji-Won Jeon, Kyu-Won Kang, Woo-Keyoung Kim, Sook Yang, Byung-Jae Kang","doi":"10.4142/jvs.23241","DOIUrl":"10.4142/jvs.23241","url":null,"abstract":"<p><strong>Background: </strong>Sufficient surgical resection is necessary for effective tumor control, but is usually limited for vertebral tumors, especially in the cervical spine in small animal neurosurgery.</p><p><strong>Objective: </strong>To evaluate the primary stability and safety of customized three-dimensional (3D)-printed implants for cervical spine reconstruction after total vertebrectomy.</p><p><strong>Methods: </strong>Customized guides and implants were designed based on computed tomography (CT) imaging of five beagle cadavers and were 3D-printed. They were used to reconstruct C5 after total vertebrectomy. Postoperative CT images were obtained to evaluate the safety and accuracy of screw positioning. After harvesting 10 vertebral specimens (C3-C7) from intact (group A) and implanted spines (group B), implant stability was analyzed using a 4-point bending test comparing with groups A and C (reconstituted with plate and pins/polymethylmethacrylate after testing in Group A).</p><p><strong>Results: </strong>All customized implants were applied without gross neurovascular damage. In addition, 90% of the screws were in a safe area, with 7.5% in grade 1 (< 1.3 mm) and 2.5% in grade 2 (> 1.3 mm). The mean entry point and angular deviations were 0.81 ± 0.43 mm and 6.50 ± 5.11°, respectively. Groups B and C significantly decreased the range of motion (ROM) in C3-C7 compared with intact spines (<i>p</i> = 0.033, and 0.018). Both groups reduced overall ROM and neutral zone in C4-C6, but only group B showed significance (<i>p</i> = 0.005, and 0.027).</p><p><strong>Conclusion: </strong>Customized 3D-printed implants could safely and accurately replace a cervical vertebra in dog cadavers while providing primary stability.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"25 1","pages":"e2"},"PeriodicalIF":1.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10839172/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139692160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Combined antimicrobial effect of two peptide nucleic acids against Staphylococcus aureus and S. pseudintermedius veterinary isolates. 两种肽核酸对金黄色葡萄球菌和假金黄色葡萄球菌兽医分离株的联合抗菌作用。
IF 1.8 3区 农林科学
Journal of Veterinary Science Pub Date : 2024-01-01 DOI: 10.4142/jvs.23265
Se Kye Kim, Jun Bong Lee, Hyung Tae Lee, Jang Won Yoon
{"title":"Combined antimicrobial effect of two peptide nucleic acids against <i>Staphylococcus aureus</i> and <i>S. pseudintermedius</i> veterinary isolates.","authors":"Se Kye Kim, Jun Bong Lee, Hyung Tae Lee, Jang Won Yoon","doi":"10.4142/jvs.23265","DOIUrl":"10.4142/jvs.23265","url":null,"abstract":"<p><strong>Background: </strong><i>Staphylococcus aureus</i> and <i>S. pseudintermedius</i> are the major etiological agents of staphylococcal infections in humans, livestock, and companion animals. The misuse of antimicrobial drugs has led to the emergence of antimicrobial-resistant <i>Staphylococcus</i> spp., including methicillin-resistant <i>S. aureus</i> (MRSA) and methicillin-resistant <i>S. pseudintermedius</i> (MRSP). One novel therapeutic approach against MRSA and MRSP is a peptide nucleic acid (PNA) that can bind to the target nucleotide strands and block expression. Previously, two PNAs conjugated with cell-penetrating peptides (P-PNAs), antisense PNA (ASP)-cmk and ASP-deoD, targeting two essential genes in <i>S. aureus</i>, were constructed, and their antibacterial activities were analyzed.</p><p><strong>Objectives: </strong>This study analyzed the combined antibacterial effects of P-PNAs on <i>S. aureus</i> and <i>S. pseudintermedius</i> clinical isolates.</p><p><strong>Methods: </strong><i>S. aureus</i> ATCC 29740 cells were treated simultaneously with serially diluted ASP-cmk and ASP-deoD, and the minimal inhibitory concentrations (MICs) were measured. The combined P-PNA mixture was then treated with <i>S. aureus</i> and <i>S. pseudintermedius</i> veterinary isolates at the determined MIC, and the antibacterial effect was examined.</p><p><strong>Results: </strong>The combined treatment of two P-PNAs showed higher antibacterial activity than the individual treatments. The MICs of two individual P-PNAs were 20 and 25 μM, whereas that of the combined treatment was 10 μM. The application of a combined treatment to clinical <i>Staphylococcus</i> spp. revealed <i>S. aureus</i> isolates to be resistant to P-PNAs and <i>S. pseudintermedius</i> isolates to be susceptible.</p><p><strong>Conclusions: </strong>These observations highlight the complexity of designing ASPs with high efficacy for potential applications in treating staphylococcal infections in humans and animals.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"25 1","pages":"e12"},"PeriodicalIF":1.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10839180/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139692161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Tumor-like lymphoplasmacytic conjunctivitis in the third eyelid in a dog. 一只狗的第三眼睑出现肿瘤样淋巴浆细胞性结膜炎。
IF 1.8 3区 农林科学
Journal of Veterinary Science Pub Date : 2024-01-01 DOI: 10.4142/jvs.23222
Hyeonu Sung, Jisoo Park, Jury Kim, Seonmi Kang, Gillian C Shaw, Kangmoon Seo
{"title":"Tumor-like lymphoplasmacytic conjunctivitis in the third eyelid in a dog.","authors":"Hyeonu Sung, Jisoo Park, Jury Kim, Seonmi Kang, Gillian C Shaw, Kangmoon Seo","doi":"10.4142/jvs.23222","DOIUrl":"10.4142/jvs.23222","url":null,"abstract":"<p><p>This report aims to describe a case of tumor-like lymphoplasmacytic conjunctivitis in a 7-year-old spayed-female Pomeranian. On complete ophthalmic examination, a mass with papillary projections was noted on the bulbar surface of the right third eyelid. Debulking of the mass was performed while preserving as much of the third eyelid as possible. On the histopathological examination, the mass was diagnosed as lymphoplasmacytic conjunctivitis with mild epithelial hyperplasia. Although a slight regrowth of the mass was noted 3 weeks after surgery, intralesional injection of triamcinolone acetonide led to its disappearance. There was no further recurrence after 5 months.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"25 1","pages":"e16"},"PeriodicalIF":1.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10839181/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139692168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Basic concepts, recent advances, and future perspectives in the diagnosis of bovine mastitis. 牛乳腺炎诊断的基本概念、最新进展和未来展望。
IF 1.8 3区 农林科学
Journal of Veterinary Science Pub Date : 2024-01-01 DOI: 10.4142/jvs.23147
Samah Attia Algharib, Ali Sobhy Dawood, Lingli Huang, Aizhen Guo, Gang Zhao, Kaixiang Zhou, Chao Li, Jinhuan Liu, Xin Gao, Wanhe Luo, Shuyu Xie
{"title":"Basic concepts, recent advances, and future perspectives in the diagnosis of bovine mastitis.","authors":"Samah Attia Algharib, Ali Sobhy Dawood, Lingli Huang, Aizhen Guo, Gang Zhao, Kaixiang Zhou, Chao Li, Jinhuan Liu, Xin Gao, Wanhe Luo, Shuyu Xie","doi":"10.4142/jvs.23147","DOIUrl":"10.4142/jvs.23147","url":null,"abstract":"<p><p>Mastitis is one of the most widespread infectious diseases that adversely affects the profitability of the dairy industry worldwide. Accurate diagnosis and identification of pathogens early to cull infected animals and minimize the spread of infection in herds is critical for improving treatment effects and dairy farm welfare. The major pathogens causing mastitis and pathogenesis are assessed first. The most recent and advanced strategies for detecting mastitis, including genomics and proteomics approaches, are then evaluated . Finally, the advantages and disadvantages of each technique, potential research directions, and future perspectives are reported. This review provides a theoretical basis to help veterinarians select the most sensitive, specific, and cost-effective approach for detecting bovine mastitis early.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"25 1","pages":"e18"},"PeriodicalIF":1.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10839174/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139692159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Exploring preventive factors against insufficient antibody positivity rate for foot-and-mouth disease in pig farms in South Korea: a preliminary ecological study. 探索韩国养猪场口蹄疫抗体阳性率不足的预防因素:一项初步生态研究。
IF 1.8 3区 农林科学
Journal of Veterinary Science Pub Date : 2024-01-01 DOI: 10.4142/jvs.23185
Dongwoon Han, Byeongwoo Ahn, Kyung-Duk Min
{"title":"Exploring preventive factors against insufficient antibody positivity rate for foot-and-mouth disease in pig farms in South Korea: a preliminary ecological study.","authors":"Dongwoon Han, Byeongwoo Ahn, Kyung-Duk Min","doi":"10.4142/jvs.23185","DOIUrl":"10.4142/jvs.23185","url":null,"abstract":"<p><strong>Background: </strong>Foot-and-mouth disease (FMD) is a highly contagious viral disease in livestock that has tremendous economic impact nationally. After multiple FMD outbreaks, the South Korean government implemented a vaccination policy for efficient disease control. However, during active surveillance by quarantine authorities, pig farms have reported an insufficient antibody positivity rate to FMD.</p><p><strong>Objective: </strong>In this study, the spatial and temporal trends of insufficiency among pig farms were analyzed, and the effect of the number of government veterinary officers was explored as a potential preventive factor.</p><p><strong>Methods: </strong>Various data were acquired, including national-level surveillance data for antibody insufficiency from the Korea Animal Health Integrated System, the number of veterinary officers, and the number of local pig farms. Temporal and geographical descriptive analyses were conducted to overview spatial and temporal trends. Additionally, logistic regression models were employed to investigate the association between the number of officers per pig farm with antibody insufficiency. Spatial cluster analysis was conducted to detect spatial clusters.</p><p><strong>Results: </strong>The results showed that the incidence of insufficiency tended to decrease in recent years (odds ratio [OR], 0.803; 95% confidence interval [95% CIs], 0.721-0.893), and regions with a higher density of governmental veterinary officers (OR, 0.942; 95% CIs, 0.918-0.965) were associated with a lower incidence.</p><p><strong>Conclusions: </strong>This study implies that previously conducted national interventions would be effective, and the quality of government-provided veterinary care could play an important role in addressing the insufficient positivity rate of antibodies.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"25 1","pages":"e13"},"PeriodicalIF":1.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10839178/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139692164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In vitro evaluation of the antitumor activity of axitinib in canine mammary gland tumor cell lines. 体外评估阿西替尼在犬乳腺肿瘤细胞系中的抗肿瘤活性。
IF 1.8 3区 农林科学
Journal of Veterinary Science Pub Date : 2024-01-01 DOI: 10.4142/jvs.23191
Hye-Gyu Lee, Ga-Hyun Lim, Ju-Hyun An, Su-Min Park, Kyoung-Won Seo, Hwa-Young Youn
{"title":"<i>In vitro</i> evaluation of the antitumor activity of axitinib in canine mammary gland tumor cell lines.","authors":"Hye-Gyu Lee, Ga-Hyun Lim, Ju-Hyun An, Su-Min Park, Kyoung-Won Seo, Hwa-Young Youn","doi":"10.4142/jvs.23191","DOIUrl":"10.4142/jvs.23191","url":null,"abstract":"<p><strong>Background: </strong>Axitinib, a potent and selective inhibitor of vascular endothelial growth factor (VEGF) receptor (VEGFR) tyrosine kinase 1,2 and 3, is used in chemotherapy because it inhibits tumor angiogenesis by blocking the VEGF/VEGFR pathway. In veterinary medicine, attempts have been made to apply tyrosine kinase inhibitors with anti-angiogenic effects to tumor patients, but there are no studies on axitinib in canine mammary gland tumors (MGTs).</p><p><strong>Objectives: </strong>This study aimed to confirm the antitumor activity of axitinib in canine mammary gland cell lines.</p><p><strong>Methods: </strong>We treated canine MGT cell lines (CIPp and CIPm) with axitinib and conducted CCK, wound healing, apoptosis, and cell cycle assays. Additionally, we evaluated the expression levels of angiogenesis-associated factors, including <i>VEGFs</i>, <i>PDGF-A</i>, <i>FGF-2</i>, and <i>TGF-β1</i>, using quantitative real-time polymerase chain reaction. Furthermore, we collected canine peripheral blood mononuclear cells (PBMCs), activated them with concanavalin A (ConA) and lipopolysaccharide (LPS), and then treated them with axitinib to investigate changes in viability.</p><p><strong>Results: </strong>When axitinib was administered to CIPp and CIPm, cell viability significantly decreased at 24, 48, and 72 h (<i>p</i> < 0.001), and migration was markedly reduced (6 h, <i>p</i> < 0.05; 12 h, <i>p</i> < 0.005). The apoptosis rate significantly increased (<i>p</i> < 0.01), and the G2/M phase ratio showed a significant increase (<i>p</i> < 0.001). Additionally, there was no significant change in the viability of canine PBMCs treated with LPS and ConA.</p><p><strong>Conclusion: </strong>In this study, we confirmed the antitumor activity of axitinib against canine MGT cell lines. Accordingly, we suggest that axitinib can be applied as a new treatment for patients with canine MGTs.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"25 1","pages":"e1"},"PeriodicalIF":1.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10839173/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139692157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Salmonella vector induces protective immunity against Lawsonia and Salmonella in murine model using prokaryotic expression system. 沙门氏菌载体利用原核表达系统在小鼠模型中诱导针对劳森氏菌和沙门氏菌的保护性免疫。
IF 1.8 3区 农林科学
Journal of Veterinary Science Pub Date : 2024-01-01 DOI: 10.4142/jvs.23219
Sungwoo Park, Eunseok Cho, Amal Senevirathne, Hak-Jae Chung, Seungmin Ha, Chae-Hyun Kim, Seogjin Kang, John Hwa Lee
{"title":"<i>Salmonella</i> vector induces protective immunity against <i>Lawsonia</i> and <i>Salmonella</i> in murine model using prokaryotic expression system.","authors":"Sungwoo Park, Eunseok Cho, Amal Senevirathne, Hak-Jae Chung, Seungmin Ha, Chae-Hyun Kim, Seogjin Kang, John Hwa Lee","doi":"10.4142/jvs.23219","DOIUrl":"10.4142/jvs.23219","url":null,"abstract":"<p><strong>Background: </strong><i>Lawsonia intracellularis</i> is the causative agent of proliferative enteropathy and is associated with several outbreaks, causing substantial economic loss to the porcine industry.</p><p><strong>Objectives: </strong>In this study, we focused on demonstrating the protective effect in the mouse model through the immunological bases of two vaccine strains against porcine proliferative enteritis.</p><p><strong>Methods: </strong>We used live-attenuated <i>Salmonella</i> Typhimurium (ST) secreting two selected immunogenic LI antigens (<i>Lawsonia</i> autotransporter A epitopes and flagellin [FliC]-peptidoglycan-associated lipoprotein-FliC) as the vaccine carrier. The constructs were cloned into a <i>Salmonella</i> expression vector (pJHL65) and transformed into the ST strain (JOL912). The expression of immunogenic proteins within <i>Salmonella</i> was evaluated via immunoblotting.</p><p><strong>Results: </strong>Immunizing BALB/c mice orally and subcutaneously induced high levels of LI-specific systemic immunoglobulin G and mucosal secretory immunoglobulin A. In immunized mice, there was significant upregulation of interferon-γ and interleukin-4 cytokine mRNA and an increase in the subpopulations of cluster of differentiation (CD) 4<sup>+</sup> and CD 8<sup>+</sup> T lymphocytes upon splenocytes re-stimulation with LI antigens. We observed significant protection in C57BL/6 mice against challenge with 10<sup>6.9</sup> times the median tissue culture infectious dose of LI or 2 × 10<sup>9</sup> colony-forming units of the virulent ST strain. Immunizing mice with either individual vaccine strains or co-mixture inhibited bacterial proliferation, with a marked reduction in the percentage of mice shedding <i>Lawsonia</i> in their feces.</p><p><strong>Conclusions: </strong><i>Salmonella</i>-mediated LI gene delivery induces robust humoral and cellular immune reactions, leading to significant protection against LI and salmonellosis.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"25 1","pages":"e4"},"PeriodicalIF":1.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10839175/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139692158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Intramammary preparation of enrofloxacin hydrochloride-dihydrate for bovine mastitis (biofilm-forming Staphylococcus aureus). 盐酸恩诺沙星二水合物乳内制剂用于治疗牛乳腺炎(生物膜形成金黄色葡萄球菌)。
IF 1.8 3区 农林科学
Journal of Veterinary Science Pub Date : 2024-01-01 DOI: 10.4142/jvs.23245
Diana Córdova-González, Edgar Alfonseca-Silva, Lilia Gutiérrez, Graciela Tapia-Pérez, Héctor Sumano
{"title":"Intramammary preparation of enrofloxacin hydrochloride-dihydrate for bovine mastitis (biofilm-forming <i>Staphylococcus aureus)</i>.","authors":"Diana Córdova-González, Edgar Alfonseca-Silva, Lilia Gutiérrez, Graciela Tapia-Pérez, Héctor Sumano","doi":"10.4142/jvs.23245","DOIUrl":"10.4142/jvs.23245","url":null,"abstract":"<p><strong>Background: </strong>Chronic bovine mastitis is linked to biofilm-producing <i>Staphylococcus aureus</i> (bp-Sa) or <i>Staphylococcus</i> coagulase-negative (bp-Scn).</p><p><strong>Objectives: </strong>Bp-Sa and bp-Scn were treated with intramammary preparations of either enrofloxacin HCl·2H<sub>2</sub>O-dimethyl-sulfoxide-chitosan (enro-C/DMSO/chitosan) or enro-C alone. Their potential to inhibit and degrade biofilm formation <i>in vitro</i> was also assessed.</p><p><strong>Methods: </strong>Milk samples were obtained from the affected quarters in a herd. Phenotypical and genotypical identifications as biofilm-producing <i>Staphylococcus</i> species were carried out. Enro-C/DMSO/chitosan and enro-C alone were assessed to determine their <i>in vitro</i> efficacy in interfering with biofilm formation and their bactericidal effects. A prolonged eight-day treatment with a twice-daily intramammary insertion of 10 mL of enro-C/DMSO/chitosan or enro-C alone was set to evaluate the clinical and bacteriological cures on day 10 in 15 cows per group and the biofilm-inhibiting ability.</p><p><strong>Results: </strong>Fifty-seven percent of the isolates were identified as <i>Staphylococcus</i> spp., of which 50% were bp-Sa, 46% bp-Scn, and 4% <i>Staphylococcus pseudintermedius</i>. One hundred percent of the <i>S. aureus</i> isolated and 77% of <i>Staphylococcus</i> coagulase-negative were biofilm producers. In both groups, the <i>icaA</i> and <i>icaD</i> biofilm-producing genes were identified. The experimental preparation could inhibit biofilm formation, degrade mature biofilms, and have well-defined microbicidal effects on planktonic and biofilm bacteria. The respective clinical and bacteriological cure rates were 100% and 80% for enro-C/DMSO/chitosan and 41.7% and 25% for enro-C alone.</p><p><strong>Conclusions: </strong>Enro-C/DMSO/chitosan eliminates bp-Sa and bp-Scn from cases of chronic bovine mastitis.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"25 1","pages":"e6"},"PeriodicalIF":1.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10839182/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139692165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evolution and international transmission of H3N2 canine influenza A viruses from Korea during 2014-2017. 2014-2017年韩国H3N2犬甲型流感病毒的进化和国际传播。
IF 1.8 3区 农林科学
Journal of Veterinary Science Pub Date : 2023-11-01 Epub Date: 2023-10-05 DOI: 10.4142/jvs.23105
Chung-Young Lee
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