Journal of Veterinary Science最新文献

{"title":"Effect of humidity on postmortem changes in rats.","authors":"Seroc Oh, Byeongwoo Ahn, Beom Jun Lee, Sang-Yoon Nam","doi":"10.4142/jvs.23327","DOIUrl":"10.4142/jvs.23327","url":null,"abstract":"<p><strong>Importance: </strong>In veterinary forensic science, accurately determining the postmortem interval (PMI) is crucial for identifying the causes of animal deaths. Autolysis, a significant postmortem process, influences PMI estimation, but its relationship with humidity is not well understood.</p><p><strong>Objective: </strong>This study aimed to improve the accuracy of PMI estimates in veterinary forensic cases by looking into how different humidity levels affect autolysis in different organs of rats.</p><p><strong>Methods: </strong>The study involved 38 male rats, examining histopathological changes in their heart, liver, and pancreas. These organs were subjected to controlled humidity levels (20%, 55%, and 80%) at a constant 22°C. Tissue samples were collected at several intervals (0 h, 12 h, 24 h, 3 days, and 8 days) for comprehensive analysis.</p><p><strong>Results: </strong>Distinct autolytic characteristics in animal organs emerged under varying humidity conditions. The low-humidity environment rapidly activated autolysis more than the high-humidity environment. In addition, it was found that lower humidity caused nuclear pyknosis, cytoplasmic disintegration, and myofiber interruption. The liver, in particular, showed portal triad aggregation and hepatocyte individuation. The pancreas experienced cell fragmentation and an enlarged intracellular space. High humidity also caused the loss of striations in cardiac tissues, and the liver showed vacuolation. Under these conditions, the pancreas changed eosinophilic secretory granules.</p><p><strong>Conclusions and relevance: </strong>The study successfully established a clear connection between the autolytic process in PMIs and relative humidity. These findings are significant for developing a more accurate and predictable method for PMI estimation in the field of veterinary forensic science.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"25 3","pages":"e24"},"PeriodicalIF":1.8,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11156596/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141247794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness of cephalosporins in hydrolysis and inhibition of <i>Staphylococcus aureus</i> and <i>Escherichia coli</i> biofilms.","authors":"Jawaria Aslam, Hafiz Muhammad Ali, Shujaat Hussain, Muhammad Zishan Ahmad, Abu Baker Siddique, Muhammad Shahid, Mirza Imran Shahzad, Hina Fatima, Sarah Tariq, Fatima Sadiq, Maria Aslam, Umar Farooq, Saadiya Zia, Rawa Saad Aljaluod, Khaloud Mohammed Alarjani","doi":"10.4142/jvs.23258","DOIUrl":"10.4142/jvs.23258","url":null,"abstract":"<p><strong>Importance: </strong><i>Staphylococcus aureus</i> and <i>Escherichia coli</i> contribute to global health challenges by forming biofilms, a key virulence element implicated in the pathogenesis of several infections.</p><p><strong>Objective: </strong>The study examined the efficacy of various generations of cephalosporins against biofilms developed by pathogenic <i>S. aureus</i> and <i>E. coli</i>.</p><p><strong>Methods: </strong>The development of biofilms by both bacteria was assessed using petri-plate and microplate methods. Biofilm hydrolysis and inhibition were tested using first to fourth generations of cephalosporins, and the effects were analyzed by crystal violet staining and phase contrast microscopy.</p><p><strong>Results: </strong>Both bacterial strains exhibited well-developed biofilms in petri-plate and microplate assays. Cefradine (first generation) showed 76.78% hydrolysis of <i>S. aureus</i> biofilm, while significant hydrolysis (59.86%) of <i>E. coli</i> biofilm was observed by cefipime (fourth generation). Similarly, cefuroxime, cefadroxil, cefepime, and cefradine caused 78.8%, 71.63%, 70.63%, and 70.51% inhibition of the <i>S. aureus</i> biofilms, respectively. In the case of <i>E. coli,</i> maximum biofilm inhibition (66.47%) was again shown by cefepime. All generations of cephalosporins were more effective against <i>S. aureus</i> than <i>E. coli</i>, which was confirmed by phase contrast microscopy.</p><p><strong>Conclusions and relevance: </strong>Cephalosporins exhibit dual capabilities of hydrolyzing and inhibiting <i>S. aureus</i> and <i>E. coli</i> biofilms. First-generation cephalosporins exhibited the highest inhibitory activity against <i>S. aureus</i>, while the third and fourth generations significantly inhibited <i>E. coli</i> biofilms. This study highlights the importance of tailored antibiotic strategies based on the biofilm characteristics of specific bacterial strains.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"25 3","pages":"e47"},"PeriodicalIF":1.8,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11156599/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141247840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole genome sequencing analysis on antibiotic-resistant <i>Escherichia coli</i> isolated from pig farms in Banten Province, Indonesia.","authors":"Hadri Latif, Debby Fadhilah Pazra, Chaerul Basri, I Wayan Teguh Wibawan, Puji Rahayu","doi":"10.4142/jvs.24031","DOIUrl":"10.4142/jvs.24031","url":null,"abstract":"<p><strong>Importance: </strong>The emergence and rapid increase in the incidence of multidrug-resistant (MDR) bacteria in pig farms has become a serious concern and reduced the choice of effective antibiotics.</p><p><strong>Objective: </strong>This study analyzed the phylogenetics and diversity of antibiotic resistance genes (ARGs) and molecularly identified the source of ARGs in antibiotic-resistant <i>Escherichia coli</i> isolated from pig farms in Banten Province, Indonesia.</p><p><strong>Methods: </strong>Forty-four antibiotic-resistant <i>E. coli</i> isolates from fecal samples from 44 pig farms in Banten Province, Indonesia, were used as samples. The samples were categorized into 14 clusters. Sequencing was performed using the Oxford Nanopore Technologies MinION platform, with barcoding before sequencing with Nanopore Rapid sequencing gDNA-barcoding (SQK-RBK110.96) according to manufacturing procedures. ARG detection was conducted using ResFinder, and the plasmid replicon was determined using PlasmidFinder.</p><p><strong>Results: </strong>Three phylogenetic leaves of <i>E. coli</i> were identified in the pig farming cluster in Banten Province. The <i>E. coli</i> isolates exhibited potential resistance to nine classes of antibiotics. Fifty-one ARGs were identified across all isolates, with each cluster carrying a minimum of 10 ARGs. The <i>ant(3'')-Ia</i> and <i>qnrS1</i> genes were present in all isolates. ARGs in the <i>E. coli</i> pig farming cluster originated mainly from plasmids, accounting for an average of 89.4%.</p><p><strong>Conclusions and relevance: </strong>The elevated potential for MDR events, coupled with the dominance of ARGs originating from plasmids, increases the risk of ARG spread among bacterial populations in animals, humans, and the environment.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"25 3","pages":"e44"},"PeriodicalIF":1.8,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11156600/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141247894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chloride and lactate as prognostic indicators of calf diarrhea from eighty-nine cases.","authors":"Gencay Ekinci, Emre Tüfekçi, Youssouf Cissé, İlknur Karaca Bekdik, Ali Cesur Onmaz, Öznur Aslan, Vehbi Güneş, Mehmet Çitil, İhsan Keleş","doi":"10.4142/jvs.23155","DOIUrl":"10.4142/jvs.23155","url":null,"abstract":"<p><strong>Importance: </strong>Deaths due to neonatal calf diarrhea are still one of the most critical problems of cattle breeding worldwide. Determining the parameters that can predict diarrhea-related deaths in calves is especially important in terms of prognosis and treatment strategies for the disease.</p><p><strong>Objective: </strong>The primary purpose of this study was to determine mortality rates and durations, survival status, and predictive prognosis parameters based on vital signs, hematology, and blood gas analyses in neonatal diarrheic calves.</p><p><strong>Methods: </strong>The hospital automation system retrospectively obtained data from 89 neonatal diarrheic calves.</p><p><strong>Results: </strong>It was found that 42.7% (38/89) of the calves brought with the complaint of diarrhea died during hospitalization or after discharge. Short-term and long-term fatalities were a median of 9.25 hours and a median of 51.50 hours, respectively. When the data obtained from this study is evaluated, body temperature (°C), pH, base excess (mmol/L), and sodium bicarbonate (mmol/L) parameters were found to be lower, and hemoglobin (g/dL), hematocrit (%), lactate (mmol/L), chloride (mmol/L), sodium (mmol/L) and anion gap (mmol/L) parameters were found to be higher in dead calves compared to survivors. Accordingly, hypothermia, metabolic acidosis, and dehydration findings were seen as clinical conditions that should be considered. Logistic regression analysis showed that lactate (odds ratio, 1.429) and CI^- (odds ratio, 1.232) concentration were significant risk factors associated with death in calves with diarrhea.</p><p><strong>Conclusions and relevance: </strong>According to the findings obtained from this study, the determination of lactate and Cl^- levels can be used as an adjunctive supplementary test in distinguishing calves with diarrhea with a good prognosis.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"25 3","pages":"e38"},"PeriodicalIF":1.8,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11156601/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141247726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Silencing subolesin and enolase impairs gene expression, engorgement and reproduction in <i>Haemaphysalis longicornis</i> (Acari: Ixodidae) ticks.","authors":"Md Samiul Haque, Mohammad Saiful Islam, Myung-Jo You","doi":"10.4142/jvs.24039","DOIUrl":"10.4142/jvs.24039","url":null,"abstract":"<p><strong>Importance: </strong><i>Haemaphysalis longicornis</i> is an obligate blood-sucking ectoparasite that has gained attention due its role of transmitting medically and veterinary significant pathogens and it is the most common tick species in Republic of Korea. The preferred strategy for controlling ticks is a multi-antigenic vaccination. Testing the efficiency of a combination antigen is a promising method for creating a tick vaccine.</p><p><strong>Objective: </strong>The aim of the current research was to analyze the role of subolesin and enolase in feeding and reproduction of <i>H. longicornis</i> by gene silencing.</p><p><strong>Methods: </strong>In this study, we used RNA interference to silence salivary enolase and subolesin in <i>H. longicornis</i>. Unfed female ticks injected with double-stranded RNA targeting subolesin and enolase were attached and fed normally on the rabbit's ear. Real-time polymerase chain reaction was used to confirm the extent of knockdown.</p><p><strong>Results: </strong>Ticks in the subolesin or enolase dsRNA groups showed knockdown rates of 80% and 60% respectively. Ticks in the combination dsRNA (subolesin and enolase) group showed an 80% knockdown. Knockdown of subolesin and enolase resulted in significant depletion in feeding, blood engorgement weight, attachment rate, and egg laying. Silencing of both resulted in a significant (<i>p</i> < 0.05) reduction in tick engorgement, egg laying, egg hatching (15%), and reproduction.</p><p><strong>Conclusions and relevance: </strong>Our results suggest that subolesin and enolase are an exciting target for future tick control strategies.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"25 3","pages":"e43"},"PeriodicalIF":1.5,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11156603/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141247801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histopathological evaluation of the lungs in experimental autoimmune encephalomyelitis.","authors":"Sungmoo Hong, Jeongtae Kim, Kyungsook Jung, Meejung Ahn, Changjong Moon, Yoshihiro Nomura, Hiroshi Matsuda, Akane Tanaka, Hyohoon Jeong, Taekyun Shin","doi":"10.4142/jvs.23302","DOIUrl":"10.4142/jvs.23302","url":null,"abstract":"<p><strong>Importance: </strong>Experimental autoimmune encephalomyelitis (EAE) is an animal model of multiple sclerosis characterized by inflammation within the central nervous system. However, inflammation in non-neuronal tissues, including the lungs, has not been fully evaluated.</p><p><strong>Objective: </strong>This study evaluated the inflammatory response in lungs of EAE mice by immunohistochemistry and histochemistry.</p><p><strong>Methods: </strong>Eight adult C57BL/6 mice were injected with myelin oligodendrocyte glycoprotein_35-55 to induce the EAE. Lungs and spinal cords were sampled from the experimental mice at the time of sacrifice and used for the western blotting, histochemistry, and immunohistochemistry.</p><p><strong>Results: </strong>Histopathological examination revealed inflammatory lesions in the lungs of EAE mice, characterized by infiltration of myeloperoxidase (MPO)- and galectin-3-positive cells, as determined by immunohistochemistry. Increased numbers of collagen fibers in the lungs of EAE mice were confirmed by histopathological analysis. Western blotting revealed significantly elevated level of osteopontin (OPN), cluster of differentiation 44 (CD44), MPO and galectin-3 in the lungs of EAE mice compared with normal controls (<i>p</i> < 0.05). Immunohistochemical analysis revealed both OPN and CD44 in ionized calcium-binding adapter molecule 1-positive macrophages within the lungs of EAE mice.</p><p><strong>Conclusions and relevance: </strong>Taken together, these findings suggest that the increased OPN level in lungs of EAE mice led to inflammation; concurrent increases in proinflammatory factors (OPN and galectin-3) caused pulmonary impairment.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"25 3","pages":"e35"},"PeriodicalIF":1.8,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11156594/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141247806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Equine helminths: prevalence and associated risk factors in Gamo Gofa Zone, Ethiopia.","authors":"Yared Abate Getahun, Bekahegn Simeon Tsalke, Abreham Wondimu Buzuneh, Mekoya Mereta Mejo, Wondyfraw Tsegaw Habtewold","doi":"10.4142/jvs.23291","DOIUrl":"10.4142/jvs.23291","url":null,"abstract":"<p><strong>Importance: </strong>Equines are indispensable in reducing the huge burden on children and women and income generation. On the other hand, minimal attention is given to improving their health and welfare.</p><p><strong>Objective: </strong>This study examined the prevalence and associated risk factors of helminth parasites of equine in the Gamo Gofa Zone.</p><p><strong>Methods: </strong>A cross-sectional study was employed from June 2019 to March 2020. The study districts and <i>Kebeles</i> were selected purposively based on agroecology whereas selection of study households and animals were performed based on simple random sampling techniques. Identification of nematode, trematode parasite ova and larvae of <i>D. arnfieldi</i> were done by floatation, sedimentation, and Baermann techniques respectively. Descriptive statistics and logistic regression was applied to estimate the prevalence and association of risk factors with helminth parasites.</p><p><strong>Results: </strong>The overall helminth parasite prevalence in the study area was 90.4%, 425/470 (95% [CI], 87.16-92.9). The prevalence of Strongyle, Fasciola, <i>O. equi</i>, <i>P. equorum</i>, <i>D. arnfieldi</i>, and mixed parasite infections were 65.1%, 21.7%, 17.4%, 34%, 34%, and 58.1%, respectively. Infections from Fasciola species and <i>D. arnfieldi</i> infection were four ([AOR], 4.4; 95% CI, 2-9.4) and two times (AOR, 2; 95% CI, 1.1-3.6) respectively more likely occur in donkeys than in mules. The occurrence of Strongyle species in midland agroecology was two times (AOR, 2.6; 95% CI, 1.4-4.7) more likely than lowland agroecology.</p><p><strong>Conclusions and relevance: </strong>The present study identified diverse species of equine helminth parasites that necessitate urgent disease control and prevention measures.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"25 3","pages":"e41"},"PeriodicalIF":1.8,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11156592/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141247846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Symmetric dimethylarginine correlates with the urea, creatinine, potassium, and clinical scores in feline urethral obstructions.","authors":"Jéssica Cavalcante da Nóbrega, Heloíse Rangel Dinallo, Silvano Salgueiro Geraldes, Maria Gabriela Picelli de Azevedo, Reiner Silveira de Moraes, Suellen Rodrigues Maia, Fabiana Ferreira de Souza, Alessandra Melchert, Henry David Mogollón García, Raphael Lúcio Andreatti Filho, Adriano Sakai Okamoto, Priscylla Tatiana Chalfun Guimarães Okamoto","doi":"10.4142/jvs.23268","DOIUrl":"https://doi.org/10.4142/jvs.23268","url":null,"abstract":"<p><strong>Background: </strong>A urethral obstruction (UO) is an emergency commonly observed in male cats, which can result in significant clinical and laboratory alterations, leading to complications and death.</p><p><strong>Objectives: </strong>This study aimed to correlate symmetric dimethylarginine (SDMA) with the urea, creatinine, potassium, and bicarbonate levels in cats with UO. In addition, the correlation between clinical score and time of obstruction was evaluated.</p><p><strong>Methods: </strong>Thirty male cats were selected and allocated into a control group (CG, n = 13) and an obstruction group (OG, n = 17). The laboratory analyses were conducted before treatment (M0) and at different times after treatment (12 h [M12], 24 h [M24], and 48 h [M48]). Correlations were established between SDMA and creatinine, urea, bicarbonate, potassium, time of obstruction, and the clinical score.</p><p><strong>Results: </strong>A strong correlation (r > 0.6) was observed between SDMA and creatinine, urea, and potassium in the OG. Furthermore, there was substantial agreement (kappa value) between SDMA and creatinine at M24. A higher clinical score was associated with a longer time of obstruction. In the OG, at M48, the SDMA and creatinine levels were 50% and 41.2% higher, respectively.</p><p><strong>Conclusions: </strong>A correlation was observed between SDMA and creatinine in obstructed cats, and significant agreement between these values was observed 24 h after the unblocking treatment. A correlation among SDMA, urea, and potassium was observed. Approximately 9% more cats continued to have elevated SDMA levels after 48 h of treatment compared to creatinine. This suggests a slightly lower sensitivity of the latter biomarker but does not exclude the possibility of congruent and normalized values after a longer evaluation period.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"25 2","pages":"e27"},"PeriodicalIF":1.8,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10990919/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140867610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and characterization of feline endometrial mesenchymal stem cells.","authors":"Mi-Kyung Park, Kun-Ho Song","doi":"10.4142/jvs.23267","DOIUrl":"https://doi.org/10.4142/jvs.23267","url":null,"abstract":"<p><strong>Background: </strong>Recently, there has been a growing interest in stem cells for human medicine. Limited feline endometrial mesenchymal stem cell (fEM-MSC) research in veterinary medicine necessitates reporting for future feline disease research and therapy.</p><p><strong>Objectives: </strong>This study aimed to isolate fEM-MSCs from feline endometrial tissues and evaluate their morphology, proliferative ability, differentiation ability, and immunophenotype.</p><p><strong>Methods: </strong>Feline endometrial tissues were obtained from the ovariohysterectomies of healthy cats and isolated using an enzymatic method. The morphology and proliferative ability of the isolated cells were assessed using a doubling time (DT) assay from passages 3 to 6 (P3 - P6). We measured pluripotency gene expressions of cells in P2 using quantitative real-time polymerase chain reaction (qRT-PCR). To investigate MSC characteristics, a trilineage differentiation assay was conducted in P4, and cells in P4 were immunophenotyped using flow cytometry.</p><p><strong>Results: </strong>fEM-MSCs showed a typical spindle-shaped morphology under a microscope, and the DT was maintained from P3 to P6. fEM-MSCs could differentiate into adipocytes, osteoblasts, and chondrocytes, and expressed three pluripotency markers (OCT4, SOX2, and NANOG) by qRT-PCR. Immunophenotypic analysis showed that the fEM-MSCs were CD14 ^-, CD34 ^-, CD45 ^-, CD9⁺, and CD44⁺.</p><p><strong>Conclusions: </strong>In this study, the feline endometrium was a novel source of MSCs, and to the best of our knowledge, this is the first report on the isolation method and characteristics of fEM-MSCs.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"25 2","pages":"e31"},"PeriodicalIF":1.8,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10990916/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140870643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical evaluation and surgical intervention for diaphragmatic eventration mimicking peritoneopericardial hernia in a cat.","authors":"Min-Jung Ko, Kyoung-Min Song, Hun-Young Yoon","doi":"10.4142/jvs.23277","DOIUrl":"https://doi.org/10.4142/jvs.23277","url":null,"abstract":"<p><p>A 2-year-old spayed female British Shorthair cat presented with an increased frequency and duration of cough since infant period. Based on radiographic, ultrasonographic, and computed tomography findings, peritoneopericardial diaphragmatic hernia was considered so that repair surgery was planned. During celiotomy, lax diaphragm was identified instead of defect. Transabdominal diaphragmatic plication was performed to resolve lax diaphragm and to prevent recurrence by overlapping relatively normal part of diaphragm. Diagnosed with diaphragmatic eventration postoperatively, the cat showed improvement in clinical signs and imaging results. Transabdominal diaphragmatic plication is a suitable treatment; the patient maintained normally during a 14-month follow-up period.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"25 2","pages":"e19"},"PeriodicalIF":1.8,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10990918/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140850225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}