Journal of Toxicologic Pathology最新文献_第8页

Honorary Member, Michihito Takahashi, M.D., Ph.D., 1937-2022. 名誉会员，高桥道仁，医学博士，1937-2022。

IF 1.2 4区医学

Journal of Toxicologic Pathology Pub Date : 2022-10-01 Epub Date: 2022-10-20 DOI: 10.1293/tox.2022-m1

引用次数: 0

Early detection of urinary bladder carcinogens in rats by immunohistochemistry for γ-H2AX: a review from analyses of 100 chemicals. 通过 γ-H2AX 免疫组织化学方法早期检测大鼠膀胱致癌物：对 100 种化学物质分析的综述。

IF 0.9 4区医学

Journal of Toxicologic Pathology Pub Date : 2022-10-01 Epub Date: 2022-08-18 DOI: 10.1293/tox.2022-0061

Takeshi Toyoda, Kumiko Ogawa

{"title":"Early detection of urinary bladder carcinogens in rats by immunohistochemistry for γ-H2AX: a review from analyses of 100 chemicals.","authors":"Takeshi Toyoda, Kumiko Ogawa","doi":"10.1293/tox.2022-0061","DOIUrl":"10.1293/tox.2022-0061","url":null,"abstract":"In safety evaluations of chemicals, there is an urgent need to develop short-term methods to replace long-term carcinogenicity tests. We have reported that immunohistochemistry for γ-H2AX, a well-established biomarker of DNA damage, can detect bladder carcinogens at an early stage using histopathological specimens from 28-day repeated-dose oral toxicity studies in rats. Given the markedly low level of γ-H2AX formation in the bladder urothelium of untreated rats, an increase in γ-H2AX-positive cells following chemical exposure can be relatively easy to identify. Among the 100 compounds examined to date, bladder carcinogens can be detected with high sensitivity (33/39; 84.6%) and specificity (58/61; 95.1%). As expected, γ-H2AX formation levels tended to be high following exposure to genotoxic bladder carcinogens, whereas nongenotoxic bladder carcinogens also increased the number of γ-H2AX-positive cells, probably through secondary DNA damage associated with sustained proliferative stimulation. γ-H2AX formation in the bladder urothelium reflects species differences in susceptibility to bladder carcinogenesis between rats and mice and shows a clear dose-dependency associated with the intensity of tumor development as well as high reproducibility. Some of the bladder carcinogens that showed false-negative results in the evaluation of γ-H2AX alone could be detected by combined evaluation with immunostaining for bladder stem cell markers, including aldehyde dehydrogenase 1A1. This method may be useful for the early detection of bladder carcinogens, as it can be performed by simple addition of conventional immunostaining using formalin-fixed paraffin-embedded tissues from 28-day repeated-dose toxicity studies in rodents, which are commonly used in safety evaluations of chemical substances.","PeriodicalId":17437,"journal":{"name":"Journal of Toxicologic Pathology","volume":"35 4","pages":"283-298"},"PeriodicalIF":0.9,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/cc/6d/tox-35-283.PMC9647216.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40478344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lack of hepatocarcinogenicity of 2,2'-[1,2-ethanediylbis(oxymethylene)]bis-oxirane, 3-hydroxy-2-naphthoic acid, and acetoacetanilide in a medium-term rat liver bioassay. 在中期大鼠肝脏生物测定中，缺乏2,2'-[1,2-乙二基双(氧亚甲基)]双氧环烷，3-羟基-2-萘酸和乙酰乙酰苯胺的肝癌致癌性。

IF 1.2 4区医学

Journal of Toxicologic Pathology Pub Date : 2022-10-01 Epub Date: 2022-08-01 DOI: 10.1293/tox.2022-0010

Hiroshi Yamagata, Tsubasa Saito, Takezo Okamoto, Kensuke Satomoto, Tatsuya Mitsumoto, Atsushi Wakita, Maki Nakamura, Takahiro Hayashi, Yuichi Kuroiwa

{"title":"Lack of hepatocarcinogenicity of 2,2'-[1,2-ethanediylbis(oxymethylene)]bis-oxirane, 3-hydroxy-2-naphthoic acid, and acetoacetanilide in a medium-term rat liver bioassay.","authors":"Hiroshi Yamagata, Tsubasa Saito, Takezo Okamoto, Kensuke Satomoto, Tatsuya Mitsumoto, Atsushi Wakita, Maki Nakamura, Takahiro Hayashi, Yuichi Kuroiwa","doi":"10.1293/tox.2022-0010","DOIUrl":"https://doi.org/10.1293/tox.2022-0010","url":null,"abstract":"The carcinogenicity of 2,2'-[1,2-ethanediylbis(oxymethylene)]bis-oxirane (ethylene glycol diglycidyl ether; EGDE), 3-hydroxy-2-naphthoic acid (HNA), and acetoacetanilide (AAA) was investigated using a medium-term rat liver bioassay for an occupational safety assessment. F344 male rats were administered a single intraperitoneal injection of diethylnitrosamine (200 mg/kg body weight (bw)/day) and then starting 2 weeks later, they received EGDE at 6, 20, and 60 mg/kg bw/day, HNA at 20, 60, and 200 mg/kg bw/day, or AAA at 60, 200, and 600 mg/kg bw/day by oral gavage for 6 weeks. The animals in the positive control group received phenobarbital sodium solution (PB, 25 mg/kg bw/day) by oral gavage and those in the negative control group received a vehicle (water/corn oil) during the administration period of test substances in this model. All animals were subjected to two-thirds partial hepatectomy at week 3 and euthanized at week 8. Neither the number nor the area of hepatocellular foci positive for glutathione S-transferase placental form (GST-P) increased in any of the EGDE, HNA, or AAA treated groups. However, the number and area of GST-P-positive foci significantly increased in the positive control group treated with PB. The results indicate that EGDE, HNA, and AAA lack hepatocarcinogenicity in rats.","PeriodicalId":17437,"journal":{"name":"Journal of Toxicologic Pathology","volume":"35 4","pages":"313-320"},"PeriodicalIF":1.2,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/24/61/tox-35-313.PMC9647213.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40478342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The potential of organoids in toxicologic pathology: role of toxicologic pathologists in in vitro chemical hepatotoxicity assessment. 类器官在毒理病理学中的潜力:毒理病理学家在体外化学肝毒性评估中的作用。

IF 1.2 4区医学

Journal of Toxicologic Pathology Pub Date : 2022-07-01 Epub Date: 2022-05-23 DOI: 10.1293/tox.2022-0017

Toshinori Yoshida, Mio Kobayashi, Suzuka Uomoto, Kanami Ohshima, Emika Hara, Yoshitaka Katoh, Naofumi Takahashi, Takanori Harada, Tatsuya Usui, Mohamed Elbadawy, Makoto Shibutani

{"title":"The potential of organoids in toxicologic pathology: role of toxicologic pathologists in in vitro chemical hepatotoxicity assessment.","authors":"Toshinori Yoshida, Mio Kobayashi, Suzuka Uomoto, Kanami Ohshima, Emika Hara, Yoshitaka Katoh, Naofumi Takahashi, Takanori Harada, Tatsuya Usui, Mohamed Elbadawy, Makoto Shibutani","doi":"10.1293/tox.2022-0017","DOIUrl":"https://doi.org/10.1293/tox.2022-0017","url":null,"abstract":"The development of in vitro toxicity assessment methods using cultured cells has gained popularity for promoting animal welfare in animal experiments. Herein, we briefly discuss the current status of hepatoxicity assessment using human- and rat-derived hepatocytes; we focus on the liver organoid method, which has been extensively studied in recent years, and discuss how toxicologic pathologists can use their knowledge and experience to contribute to the development of in vitro chemical hepatotoxicity assessment methods for drugs, pesticides, and chemicals. We also propose how toxicological pathologists should assess toxicity regarding the putative distribution of undifferentiated and differentiated cells in the organoid when liver organoids are observed in hematoxylin and eosin-stained specimens. This was done while considering the usefulness and limitations of in vitro studies for toxicologic pathology assessment.","PeriodicalId":17437,"journal":{"name":"Journal of Toxicologic Pathology","volume":"35 3","pages":"225-235"},"PeriodicalIF":1.2,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/0e/e8/tox-35-225.PMC9256002.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40504084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

A case of spontaneous rete testis adenoma in a Sprague-Dawley rat. Sprague-Dawley大鼠自发性睾丸网状腺瘤一例。

IF 1.2 4区医学

Journal of Toxicologic Pathology Pub Date : 2022-07-01 Epub Date: 2022-05-07 DOI: 10.1293/tox.2022-0018

Masako Imaoka, Tetsuya Osawa, Kiyonori Kai, Yoshimi Tsuchiya

引用次数: 0

Failure to gulp surface air induces swim bladder adenomas in Japanese medaka (Oryzias latipes). 日本水豚(Oryzias latipes)因未能吞咽表面空气而诱发鱼鳔腺瘤。

IF 1.2 4区医学

Journal of Toxicologic Pathology Pub Date : 2022-07-01 Epub Date: 2022-04-21 DOI: 10.1293/tox.2022-0030

Satoshi Furukawa, Yuichiro Machida, Kazuya Takeuchi, Yumiko Hoshikawa, Kota Irie

{"title":"Failure to gulp surface air induces swim bladder adenomas in Japanese medaka (Oryzias latipes).","authors":"Satoshi Furukawa, Yuichiro Machida, Kazuya Takeuchi, Yumiko Hoshikawa, Kota Irie","doi":"10.1293/tox.2022-0030","DOIUrl":"https://doi.org/10.1293/tox.2022-0030","url":null,"abstract":"In order to elucidate the effects of swim bladder inflation failure on swim bladder carcinogenesis, we investigated the sequential histopathological changes of swim bladders at 13, 24, 35, and 53 days post-hatch (dph) in medakas with an uninflated swim bladder, which was experimentally induced by denying access to the air-water interface between 0 and 6 dph. The reactive oxygen species (ROS) levels were measured at 24 dph. An uninflated swim bladder was induced in 47.3% of the fish denied access to the air-water interface (the denied group). The total incidence of swim bladder adenoma was 54.1% in the denied group; however, these tumors were observed in all fish with an uninflated swim bladder. In fact, these tumors were observed from 13 dph and onwards. The TBARS levels of the juveniles showed a 2.6-fold increase in fish with an uninflated swim bladder in the denied group compared to that in the control group. It is speculated that swim bladder inflation failure has some effects on the gas gland to produce ROS, leading to DNA damage in the gas glandular epithelium, which develops into swim bladder adenomas. Consequently, it is concluded that denying access to the air-water interface between 0 and 6 dph in medaka is an easy method of inducing swim bladder tumors in a short-term period, and is a useful method for producing tumor-bearing fish.","PeriodicalId":17437,"journal":{"name":"Journal of Toxicologic Pathology","volume":"35 3","pages":"237-246"},"PeriodicalIF":1.2,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/0b/a9/tox-35-237.PMC9255999.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40504086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Histopathology of acute colchicine intoxication: novel findings and their association with clinical manifestations. 急性秋水仙碱中毒的组织病理学:新发现及其与临床表现的关系。

IF 1.2 4区医学

Journal of Toxicologic Pathology Pub Date : 2022-07-01 Epub Date: 2022-04-03 DOI: 10.1293/tox.2022-0007

Shojiro Ichimata, Yukiko Hata, Kojiro Hirota, Naoki Nishida

{"title":"Histopathology of acute colchicine intoxication: novel findings and their association with clinical manifestations.","authors":"Shojiro Ichimata, Yukiko Hata, Kojiro Hirota, Naoki Nishida","doi":"10.1293/tox.2022-0007","DOIUrl":"https://doi.org/10.1293/tox.2022-0007","url":null,"abstract":"A 32-year-old woman attempted suicide by ingesting Gloriosa bulbs and died approximately 2 days later. Toxicological examination revealed a potentially fatal blood concentration of colchicine (0.096 mg/L). In addition to the increased mitotic figures in the gastrointestinal mucosa, a unique finding for acute colchicine intoxication, pathological examination showed microvesicular lipid droplets in the liver, kidney, heart, and conduction system. Furthermore, central chromatolysis of neurons was observed in the pontine nucleus, medial accessory olivary nucleus, nucleus of the solitary tract, and nucleus ambiguus. Grumose degeneration of the cerebellar dentate nucleus was also evident. These pathological findings may help identify colchicine intoxication, even in the absence of evidence suggesting ingestion during autopsy. Moreover, pathological changes in the heart and central nervous system may be associated with the development of serious complications of acute colchicine intoxication.","PeriodicalId":17437,"journal":{"name":"Journal of Toxicologic Pathology","volume":"35 3","pages":"255-262"},"PeriodicalIF":1.2,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/73/8d/tox-35-255.PMC9255996.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40504087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

A simple specimen preparation method for histopathological evaluation of vestibular organs. 一种用于前庭器官组织病理学评估的简单标本制备方法。

IF 1.2 4区医学

Journal of Toxicologic Pathology Pub Date : 2022-07-01 Epub Date: 2022-05-07 DOI: 10.1293/tox.2022-0008

Shinichi Onishi, Atsuko Murai, Aki Kito, Yuka Kawashima, Yusuke Ohmori, Atsuhiko Kato

引用次数: 1

Response biomarkers of inhalation exposure to cigarette smoke in the mouse lung. 小鼠肺部吸入暴露于香烟烟雾的反应生物标志物。

IF 1.2 4区医学

Journal of Toxicologic Pathology Pub Date : 2022-07-01 Epub Date: 2022-05-21 DOI: 10.1293/tox.2021-0077

Shugo Suzuki, Kazuhisa Asai, Min Gi, Kazuya Kojima, Anna Kakehashi, Yuji Oishi, Taisuke Matsue, Nao Yukimatsu, Kazuto Hirata, Tomoya Kawaguchi, Hideki Wanibuchi

{"title":"Response biomarkers of inhalation exposure to cigarette smoke in the mouse lung.","authors":"Shugo Suzuki, Kazuhisa Asai, Min Gi, Kazuya Kojima, Anna Kakehashi, Yuji Oishi, Taisuke Matsue, Nao Yukimatsu, Kazuto Hirata, Tomoya Kawaguchi, Hideki Wanibuchi","doi":"10.1293/tox.2021-0077","DOIUrl":"https://doi.org/10.1293/tox.2021-0077","url":null,"abstract":"Cigarette smoking is known to increase the risk of cancer and chronic obstructive pulmonary disease (COPD). In this study, we evaluated the effects of short-term nose-only inhalation exposure to cigarette smoke in mice. Male 10-week-old C57BL mice were exposed to clean air (control) or mainstream cigarette smoke for 1 h/day, 5 days/week, for 2 or 4 weeks. Exposure to cigarette smoke increased the number of inflammatory cells, especially neutrophils, in the bronchoalveolar lavage fluid, increased inflammatory cell infiltration foci, and caused an increase in the thickness of the peripheral bronchial epithelium. Microarray gene expression analysis indicated that smoke exposure induced inflammatory responses, including leukocyte migration and activation of phagocytes and myeloid cells, as early as two weeks after the initiation of exposure. Importantly, chemokine (C-C motif) ligand 17, resistin-like alpha, and lipocalin 2 were upregulated and may serve as useful markers of the toxic effects of exposure to cigarette smoke before pulmonary histological changes become evident.","PeriodicalId":17437,"journal":{"name":"Journal of Toxicologic Pathology","volume":"35 3","pages":"247-254"},"PeriodicalIF":1.2,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/3d/29/tox-35-247.PMC9256000.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40504082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Histopathologic effect of in ovo exposure to methotrexate at early embryonic stage on optic tectum of Japanese quail (Coturnix japonica). 早期胚胎期卵暴露于甲氨蝶呤对日本鹌鹑(Coturnix japonica)视屏的组织病理学影响。

IF 1.2 4区医学

Journal of Toxicologic Pathology Pub Date : 2022-07-01 Epub Date: 2022-04-23 DOI: 10.1293/tox.2022-0011

Masakatsu Nohara, Mayo Iwasaki, Mahiro Nishio, Akihiko Sugiyama

引用次数: 1