Journal of Toxicologic Pathology最新文献_第10页

Nitrite exposure may induce infertility in mice 亚硝酸盐暴露可能导致小鼠不孕

IF 1.2 4区医学

Journal of Toxicologic Pathology Pub Date : 2021-12-04 DOI: 10.1293/tox.2021-0002

Shanshan Wu, Sang Hu, Wenjuan Fan, Xiao-ju Zhang, Haili Wang, Chaojie Li, J. Deng

{"title":"Nitrite exposure may induce infertility in mice","authors":"Shanshan Wu, Sang Hu, Wenjuan Fan, Xiao-ju Zhang, Haili Wang, Chaojie Li, J. Deng","doi":"10.1293/tox.2021-0002","DOIUrl":"https://doi.org/10.1293/tox.2021-0002","url":null,"abstract":"In the present study, we investigated the potential of nitrite exposure to induce infertility in mice. Adult female C57BL/6J mice were randomly divided into control and nitrite exposure groups. Subsequently, the rate of mouse infertility was calculated, and pathological changes in ovarian tissues were examined using hematoxylin and eosin staining. In addition, TUNEL staining, immunofluorescent labeling, and western blotting were performed to assess cell apoptosis and oxidative stress response in ovarian tissues from various groups. We observed that nitrite exposure could induce infertility (p<0.05) in mice. High-dose nitrite exposure caused infertility in a time-dependent manner, and two-round exposure induced higher infertility than that one-round exposure (p<0.01). In addition, a higher number of atretic follicles were detected in the ovaries of nitrite-exposed groups than in the control group. Furthermore, TUNEL-positive cells were observed in granulosa cells of atretic follicles, and overexpression of caspase 8, c-Fos, and inducible nitric oxide synthase (iNOS) was detected in ovaries after nitrite exposure (p<0.01), suggesting that cell apoptosis and oxidative stress response were induced following nitrite exposure. Collectively, these findings suggest that nitrite exposure can induce mouse infertility in a time-dependent manner. Oxidative stress response and cell apoptosis are involved in mediating nitrite-induced infertility.","PeriodicalId":17437,"journal":{"name":"Journal of Toxicologic Pathology","volume":"35 1","pages":"75 - 82"},"PeriodicalIF":1.2,"publicationDate":"2021-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43055208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Deep learning-based image-analysis algorithm for classification and quantification of multiple histopathological lesions in rat liver 基于深度学习的大鼠肝脏多组织病理病变分类与定量图像分析算法

IF 1.2 4区医学

Journal of Toxicologic Pathology Pub Date : 2021-11-27 DOI: 10.1293/tox.2021-0053

Taishi Shimazaki, Ameya Deshpande, Anindya Hajra, Tijo Thomas, Kyotaka Muta, Naohito Yamada, Y. Yasui, T. Shoda

{"title":"Deep learning-based image-analysis algorithm for classification and quantification of multiple histopathological lesions in rat liver","authors":"Taishi Shimazaki, Ameya Deshpande, Anindya Hajra, Tijo Thomas, Kyotaka Muta, Naohito Yamada, Y. Yasui, T. Shoda","doi":"10.1293/tox.2021-0053","DOIUrl":"https://doi.org/10.1293/tox.2021-0053","url":null,"abstract":"Artificial intelligence (AI)-based image analysis is increasingly being used for preclinical safety-assessment studies in the pharmaceutical industry. In this paper, we present an AI-based solution for preclinical toxicology studies. We trained a set of algorithms to learn and quantify multiple typical histopathological findings in whole slide images (WSIs) of the livers of young Sprague Dawley rats by using a U-Net-based deep learning network. The trained algorithms were validated using 255 liver WSIs to detect, classify, and quantify seven types of histopathological findings (including vacuolation, bile duct hyperplasia, and single-cell necrosis) in the liver. The algorithms showed consistently good performance in detecting abnormal areas. Approximately 75% of all specimens could be classified as true positive or true negative. In general, findings with clear boundaries with the surrounding normal structures, such as vacuolation and single-cell necrosis, were accurately detected with high statistical scores. The results of quantitative analyses and classification of the diagnosis based on the threshold values between “no findings” and “abnormal findings” correlated well with diagnoses made by professional pathologists. However, the scores for findings ambiguous boundaries, such as hepatocellular hypertrophy, were poor. These results suggest that deep learning-based algorithms can detect, classify, and quantify multiple findings simultaneously on rat liver WSIs. Thus, it can be a useful supportive tool for a histopathological evaluation, especially for primary screening in rat toxicity studies.","PeriodicalId":17437,"journal":{"name":"Journal of Toxicologic Pathology","volume":"35 1","pages":"135 - 147"},"PeriodicalIF":1.2,"publicationDate":"2021-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49046046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Malignant pinealoma observed in the deep cerebral parenchyma of a male Wistar rat 雄性Wistar大鼠脑深部实质中恶性松果体瘤的观察

IF 1.2 4区医学

Journal of Toxicologic Pathology Pub Date : 2021-11-25 DOI: 10.1293/tox.2021-0047

Mizuho Takagi, Y. Yamaguchi, S. Yamakawa, K. Tamura

引用次数: 0

Luteal toxicity evaluation in rats 大鼠黄体毒性评价

IF 1.2 4区医学

Journal of Toxicologic Pathology Pub Date : 2021-11-18 DOI: 10.1293/tox.2021-0058

Yoshikazu Taketa

{"title":"Luteal toxicity evaluation in rats","authors":"Yoshikazu Taketa","doi":"10.1293/tox.2021-0058","DOIUrl":"https://doi.org/10.1293/tox.2021-0058","url":null,"abstract":"The corpora lutea (CL) are endocrine glands that form in the ovary after ovulation and secrete the steroid hormone, progesterone (P4). P4 plays a critical role in estrous and menstrual cycles, implantation, and pregnancy. The incomplete rodent estrous cycle stably lasts 4–5 days and its morphological features can be distinguished during each estrous cycle stage. In rat ovaries, there are two main types of CL: newly formed ones due to the current ovulation (new CL), and CL remaining from prior estrous cycles (old CL). In the luteal regression process, CL were almost fully regressed after four estrous cycles in Sprague-Dawley rats. P4 secretion from CL in rodents is regulated by the balance between synthesis and catabolism. In general, luteal toxicity should be evaluated by considering antemortem and postmortem data. Daily vaginal smear observations provided useful information on luteal toxicity. In histopathological examinations, not only the ovaries and CL but also other related tissues and organs including the uterus, vagina, mammary gland, and adrenal glands, must be carefully examined for exploring luteal changes. In this review, histological and functional characteristics of CL in rats are summarized, and representative luteal toxicity changes are presented for improved luteal toxicity evaluation in preclinical toxicity research.","PeriodicalId":17437,"journal":{"name":"Journal of Toxicologic Pathology","volume":"35 1","pages":"7 - 17"},"PeriodicalIF":1.2,"publicationDate":"2021-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43907471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Lipomatosis of axillary lymph nodes in a cynomolgus monkey (Macaca fascicularis) 食蟹猴腋窝淋巴结脂肪瘤病的研究

IF 1.2 4区医学

Journal of Toxicologic Pathology Pub Date : 2021-11-15 DOI: 10.1293/tox.2021-0054

M. Nakagawa, S. Hayashi, S. Matsuo, Masaki Yamazaki, A. Kato

引用次数: 1

Porencephaly with an optic organ abnormality in a beagle dog 比格犬伴有视觉器官异常的脑孔畸形

IF 1.2 4区医学

Journal of Toxicologic Pathology Pub Date : 2021-10-25 DOI: 10.1293/tox.2021-0039

Osamu Hashiguchi, Yuko Yamaguchi, S. Ikezaki, Tsubasa Saito, Saori Igura, Ryo Hirai, N. Horiuchi, K. Tamura

引用次数: 1

A case of spontaneous autoimmune skin disease in a cynomolgus monkey (Macaca fascicularis) 食蟹猴自发性自身免疫性皮肤病1例

IF 1.2 4区医学

Journal of Toxicologic Pathology Pub Date : 2021-10-16 DOI: 10.1293/tox.2021-0048

Shinichi Onishi, M. Nakagawa, S. Matsuo, J. Shinozuka, Mio Hiramatsu, Takehito Isobe, Toshihiko Watanabe, A. Kato

{"title":"A case of spontaneous autoimmune skin disease in a cynomolgus monkey (Macaca fascicularis)","authors":"Shinichi Onishi, M. Nakagawa, S. Matsuo, J. Shinozuka, Mio Hiramatsu, Takehito Isobe, Toshihiko Watanabe, A. Kato","doi":"10.1293/tox.2021-0048","DOIUrl":"https://doi.org/10.1293/tox.2021-0048","url":null,"abstract":"Pemphigus is an autoimmune blistering disease characterized by lesions on the skin and mucous membranes. To date, no spontaneous cases of this disease have been reported in cynomolgus monkeys. This report describes the histopathological characteristics of spontaneous pemphigus in a cynomolgus monkey. Macroscopically, redness and scaling with pruritus were observed on the skin of the entire body. Histopathologically, the epidermis showed intercellular edema, and eosinophils and mononuclear cells infiltrated the epidermis. There was no obvious acantholysis in the epidermis. The perivascular area showed edema, and eosinophils and mononuclear cells infiltrated the vessels in the dermis. Immunohistochemically, the intercellular area in the epidermis was positive for Immunoglobulin G and Complement component 3. Serologically, anti-desmoglein 1 and desmoglein 3 antibodies in the serum were negative. From these findings, this case was diagnosed as an autoimmune skin disease, suspected to be pemphigus, and concluded as lesions being similar to those in human “pemphigus herpetiformis”.","PeriodicalId":17437,"journal":{"name":"Journal of Toxicologic Pathology","volume":"35 1","pages":"103 - 106"},"PeriodicalIF":1.2,"publicationDate":"2021-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45278986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Confounders for kidney carcinogenesis in rodent cancer bioassays 啮齿动物癌症生物检测中肾癌发生的混杂因素

IF 1.2 4区医学

Journal of Toxicologic Pathology Pub Date : 2021-10-14 DOI: 10.1293/tox.2021-0000

G. Hard

引用次数: 1

Evaluation of Caspase-3 and Ki-67 expression in squamous cell hyperplasia of the stomach induced by Platycodi radix water extract in Sprague–Dawley rats 桔梗水提物诱导大鼠胃鳞状细胞增生过程中Caspase-3和Ki-67的表达

IF 1.2 4区医学

Journal of Toxicologic Pathology Pub Date : 2021-10-09 DOI: 10.1293/tox.2021-0003

Yinghua Li, Seung-Beom Cha, Youngil Park, Bo-Ho Gong, Inae Jeong, H. Kim, Min-Soo Kang, Young-Suk Kim, Chang Hoon Han, Hyun-Kul Lee, Si-Whan Song, C. Park, B. Kang

{"title":"Evaluation of Caspase-3 and Ki-67 expression in squamous cell hyperplasia of the stomach induced by Platycodi radix water extract in Sprague–Dawley rats","authors":"Yinghua Li, Seung-Beom Cha, Youngil Park, Bo-Ho Gong, Inae Jeong, H. Kim, Min-Soo Kang, Young-Suk Kim, Chang Hoon Han, Hyun-Kul Lee, Si-Whan Song, C. Park, B. Kang","doi":"10.1293/tox.2021-0003","DOIUrl":"https://doi.org/10.1293/tox.2021-0003","url":null,"abstract":"Platycodi radix is widely used in traditional herbal medicine for the treatment of bronchitis, asthma, pulmonary tuberculosis, hypertension, hyperlipidemia, and diabetes. This study aimed to investigate cell proliferation (Ki-67) and apoptosis (Caspase-3) potential in squamous cell hyperplasia of the stomach induced by a Platycodi radix water extract in a subchronic toxicity study. One hundred formalin-fixed, paraffin-embedded stomach tissues of rats treated with Platycodi radix at doses of 0, 500, 1,000, and 3,000 mg/kg body weight/day were used for the analysis. They were conventionally stained using hematoxylin and eosin (H&E) and immunohistochemically (IHC) stained using caspase-3 and Ki-67 antibodies. The incidence of squamous cell hyperplasia was significantly increased in the 3,000 mg/kg b.w./day treatment group in both sexes (p<0.01). However, the hyperplastic change was completely repaired after 4 weeks of recovery period. Ki-67 expression was similar in all groups, with no statistically significant differences among the groups. Caspase-3 expression was significantly increased in both sexes in the 3,000 mg/kg b.w./day treatment group (p<0.01), compared with the vehicle control groups, and then reduced to normal levels in the recovery groups in both sexes. In conclusion, this study showed that squamous cell hyperplasia induced by the Platycodi radix water extract in the limiting ridge of the stomach is not considered to be abnormal proliferative change; as a result, squamous cell hyperplasia is considered to be a non-adverse effect when induced by the oral administration of the Platycodi radix water extract once daily for 13 weeks in rats.","PeriodicalId":17437,"journal":{"name":"Journal of Toxicologic Pathology","volume":"35 1","pages":"45 - 52"},"PeriodicalIF":1.2,"publicationDate":"2021-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46245948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Spontaneous well-differentiated pancreatic islet cell carcinoma with vascular invasion in a male F344 rat 雄性F344大鼠自发性高分化胰岛细胞癌伴血管浸润

IF 1.2 4区医学

Journal of Toxicologic Pathology Pub Date : 2021-10-08 DOI: 10.1293/tox.2021-0037

Shiori Fujiwara, Y. Yamaguchi, Tsubasa Saito, T. Nakazawa, K. Tamura

引用次数: 0