Journal of thermal biology最新文献

{"title":"Acute effects of heat intervention and hybrid exercise on protein synthesis, ribosome biogenesis and autophagy","authors":"Tom Normand-Gravier ,&nbsp;Robert Solsona ,&nbsp;Flavie Arnould ,&nbsp;Roméo Deriaz ,&nbsp;Christelle Bertrand-Gaday ,&nbsp;Fabio Borrani ,&nbsp;Henri Bernardi ,&nbsp;Anthony M.J. Sanchez","doi":"10.1016/j.jtherbio.2025.104169","DOIUrl":"10.1016/j.jtherbio.2025.104169","url":null,"abstract":"<div><div>The use of kaumatherapy (<em>i.e.</em> heat exposure like sauna-bathing) as passive intervention has become of growing interest to promote skeletal muscle adaptations such as strength gains and preservation of muscle mass. Importantly, the effects of a single exposure to heat (HE) in combination with hybrid exercises, designed to induce both aerobic and resistance adaptations (EX), on protein turnover remains unclear. The objective of this investigation was to evaluate the responses to HE, EX and their combination (EX + HE) on the expression of mRNA and protein related to proteosynthesis, ribosome biogenesis and content, as well as autophagy and cellular stress pathways. Eight-week-old male mice C57BL/6 J mice (n = 8 per condition) underwent acute HE (45min, 40 °C), EX (high-intensity inclined treadmill running) or EX + HE immediately after exercise. Mice were euthanized 240 min post-interventions and quadriceps muscles were harvested for analysis. Acute HE increased the mRNA expression of markers of ribosome biogenesis (POL1RA, UBF), but did not enhance ribosomal content (rRNA 18 S and 28 S) nor ribosomal transcription (pre-rRNA 45 S). Concerning the modulation of protein synthesis, EX induced an increase of MTORC1 downstream targets (P-S6K1, P-RPS6) and protein synthesis flux (assessed by puromycin incorporation) while HE alone did not and post-exercise HE had no additional effects. EX + HE also led to enhanced protein synthesis rates, but did not confer any additional benefit compared to EX alone. Finally, only EX + HE increased the autophagy flux marker LC3-B II/I, the microautophagy marker LAMP2A and phosphorylation level of P-NFκB Ser536. However, no changes in protein carbonylation were detected at this time point. These results suggest that acute post-exercise HE has no additional effects on protein synthesis at 4 h post-exercise but, when combined with EX, increases autophagy and P-NFκB Ser536, suggesting a heightened cellular stress response.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"131 ","pages":"Article 104169"},"PeriodicalIF":2.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144556767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AdipoR2 enhances adipocyte thermogenesis in Tibetan pigs via AMPK-ACC signaling","authors":"Siqi Liu,&nbsp;Zhen Yang,&nbsp;Yanan Yang,&nbsp;Yuan Cai,&nbsp;Shengguo Zhao","doi":"10.1016/j.jtherbio.2025.104209","DOIUrl":"10.1016/j.jtherbio.2025.104209","url":null,"abstract":"<div><div>Previous studies have demonstrated that the adiponectin receptor 2 (AdipoR2) gene is significantly upregulated in the subcutaneous inguinal adipose tissue of cold-resistant Tibetan pigs compared to cold-sensitive Landrace pigs under long-term cold exposure. Adiponectin interacts with its receptors on adipocyte membranes, initiating a signaling cascade that promotes thermogenesis. However, the specific role of AdipoR2 in thermogenesis within Tibetan pig adipocytes remains unclear. This study investigates the effects of AdipoR2 overexpression on glucose and lipid metabolism, as well as thermogenesis, in Tibetan pig adipocytes. Subcutaneous inguinal fat tissue was collected from 2-week-old Tibetan piglets, and preadipocytes were isolated through enzymatic digestion. The pcDNA3.1-<em>AdipoR2</em> overexpression plasmid was transfected into these cells, and transfection efficiency was assessed using RT-qPCR, along with the expression of genes related to glucose and lipid metabolism. Cell proliferation was evaluated using the CCK8 assay, while Western blot (WB) was employed to detect the phosphorylation of AMPK and ACC. Adipogenic differentiation capacity was assessed via Oil Red O staining, mitochondrial membrane potential was measured using JC-1 staining, and ATP production was quantified using ATP generation reagents. The results demonstrate that AdipoR2 overexpression activates the AMPK-ACC signaling pathway, leading to increased expression of glycolysis and lipolysis genes, concomitant with reduced adipogenic differentiation ability, enhanced mitochondrial respiration and ATP synthesis, and elevated thermogenesis in Tibetan pig preadipocytes. These findings provide a theoretical foundation for further exploration of the role of AdipoR2 in thermogenesis and its potential applications in improving cold adaptation in Tibetan pigs.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"131 ","pages":"Article 104209"},"PeriodicalIF":2.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144587899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Feed efficiency reduction and avian uncoupling protein expression in heat stressed broilers","authors":"A. Papanikolaou ,&nbsp;M.G.R. Matthijs ,&nbsp;S. Khempaka ,&nbsp;S. Okrathok ,&nbsp;R.M. Dwars ,&nbsp;S. van Gastelen ,&nbsp;T. Veldkamp ,&nbsp;J.T. Schonewille","doi":"10.1016/j.jtherbio.2025.104197","DOIUrl":"10.1016/j.jtherbio.2025.104197","url":null,"abstract":"","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"131 ","pages":"Article 104197"},"PeriodicalIF":2.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144587902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Validation of a novel method to quantify local axilla sweating, SweatSENSE","authors":"Andrew McGill ,&nbsp;Emmett Cullen Tinley ,&nbsp;Stephanie E. Edwards ,&nbsp;Andrew Jamieson ,&nbsp;Jane Ford ,&nbsp;Evie Winterton ,&nbsp;Jacob Shardey ,&nbsp;Rachel A. Hand ,&nbsp;Spyridon Efstathiou ,&nbsp;Alan M. Wemyss ,&nbsp;Maria Grypioti ,&nbsp;Gavin Kirby ,&nbsp;Tammie Barlow ,&nbsp;David M. Haddleton ,&nbsp;David A. Low","doi":"10.1016/j.jtherbio.2025.104199","DOIUrl":"10.1016/j.jtherbio.2025.104199","url":null,"abstract":"<div><div>The aim of this study was to validate the ability of the novel SweatSENSE technique to measure local axilla sweating. The local axilla sweating responses to a range of physical activities were measured in 30 healthy females (28 ± 5 yr, 163 ± 7 cm; 62 ± 9 kg). Participants completed low-to moderate-intensity treadmill walking, stepping, and cycling in a 3-h protocol. Left axilla sweating was intermittently recorded using the novel SweatSENSE method, in which non-hydrochromic sweat-responsive paper sensors with PCDA polymer were placed in the axilla vault for 5 s. The sensors were analysed for the proportional colour change as an index of instantaneous local sweating. Percentage total sweat coverage (%TC) of the patches was determined. Ipsilateral axilla sweating was continuously recorded using capacitance hygrometry (CH). Intraclass correlation coefficient (ICC) analyses was used to examine the agreement between the CH and SweatSENSE %TC data for each participant. Heart rate, core and skin temperatures, ratings of perceived exertion (RPE) and thermal discomfort were also recorded. Heart rate (100 ± 25 beats.min⁻¹, P &lt; 0.05) and T_core increased during the protocol (37.82 ± 0.23 °C, P &lt; 0.05). Thermal discomfort increased from ‘neutral’ to ‘warm’ and RPE increased to ‘somewhat hard’ during the protocol (both P &lt; 0.05). Local axilla sweating (CH and the SweatSENSE method) increased during each physical activity bout and progressively throughout the protocol (both P &lt; 0.05). Twenty one out of thirty participants demonstrated significant ICC between axilla SweatSENSE and capacitance hygrometry sweating data [average (95 % confidence intervals] ICC; 0.483 (0.191–0.713)) indicating a moderate agreement between SweatSENSE and capacitance hygrometry, suggesting a moderate level of validity of the SweatSENSE method.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"131 ","pages":"Article 104199"},"PeriodicalIF":2.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144665822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Imidazoline 1 receptors mediate the hyperthermia induced by preoptic agmatine application in male rats","authors":"Jianhui Xu ,&nbsp;Nanping Wang ,&nbsp;Qiaofeng Ji ,&nbsp;Yu Mo ,&nbsp;Jiajun Li ,&nbsp;Lihui Nie ,&nbsp;Yiran Peng ,&nbsp;Jie Zhang","doi":"10.1016/j.jtherbio.2025.104206","DOIUrl":"10.1016/j.jtherbio.2025.104206","url":null,"abstract":"<div><div>Previous studies have confirmed that microinjection of agmatine into the preoptic area (POA) induces hyperthermia in male rats, yet its receptor mechanism is known little. In the present study, using agonists and antagonists for imidazoline receptors (IRs) and α2-adrenoceptors (α2-ARs), to which agmatine binds with high affinity, we explored the roles of IRs and α2-ARs in hyperthermia induced by intra-POA agmatine in male rats. We found that intra-POA administration of the I1R agonist moxonidine elevated the core temperature and physical activity in conscious rats, enhanced brown adipose tissue (BAT) thermogenesis and shivering in anesthetized rats, and depressed the warm-sensitive neurons (WSNs) in POA slices <em>in vitro</em>, which aligned with previously demonstrated agmatine effects. Meanwhile, the selective I1R antagonist AGN192403 attenuated these effects. Moreover, fluorescence immunohistochemical analysis of Nischarin (a natural functional I1R) indicated that I1Rs were distributed in the POA. However, neither agonists nor antagonists for I2R or α2-ARs mimicked or attenuated the effects of intra-POA agmatine. In conclusion, we demonstrated that it is I1R rather than I2R or α2-ARs that mediates intra-POA agmatine-induced hyperthermia by elevating physical activity, BAT thermogenesis and shivering, likely through depression of POA WSNs. These results indicate an important role of the preoptic agmatine–I1R system in the thermoregulation.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"131 ","pages":"Article 104206"},"PeriodicalIF":2.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144563337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global insights from a decade of nesting data for olive ridley, green, hawksbill, and leatherback turtles: Implications for conservation in the face of climate change","authors":"Rubén Vinueza-Chérrez ,&nbsp;Antonio J. Carpio ,&nbsp;Estefanía Sánchez-Flores ,&nbsp;Marga L. Rivas","doi":"10.1016/j.jtherbio.2025.104196","DOIUrl":"10.1016/j.jtherbio.2025.104196","url":null,"abstract":"<div><div>Sea turtles have existed on Earth for approximately 150 million years, during which time they have adapted to various environmental and oceanographic changes. However, the effects of current climate change on these reptiles are of concern due to the direct impact of temperature on sex ratio and hatching success. Low temperatures generally produce male offspring, while high temperatures favor the development of females, with an upper limit of thermal tolerance ranging between 33 and 35 °C. Global warming poses both a long-term risk of feminization of sea turtle populations and a short- and medium-term risk of decreased hatching success. The objective of this study was to evaluate the reproductive parameters of four species of sea turtles (<em>Lepidochelys olivacea, Chelonia mydas, Dermochelys coriacea</em> and <em>Eretmochelys imbricata</em>) during the last decade by carrying out a systematic review, in which 100 published articles were analyzed following the PRISMA methodology. The results revealed a significant bias towards female offspring in all species, with recorded values of up to 100 % (<em>Lepidochelys olivacea</em>) and 98 % (<em>Chelonia mydas</em>). When analyzing hatching success, it was observed that no species reaches 100 % success and that <em>Chelonia mydas</em> has the highest average (68.9 %), while <em>Dermochelys coriacea</em> records the lowest values (50.4 %). It important to note that reproductive parameters vary between species and populations. The undeniable tendency towards female offspring in all species is evident. However, this dynamic may result not only from recorded temperatures but also from the evolutionary history of sea turtles.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"131 ","pages":"Article 104196"},"PeriodicalIF":2.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144549731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic and survival responses to high temperature stress in the red-haired pine bark beetle Hylurgus ligniperda","authors":"Ling Cheng ,&nbsp;Fengming Shi ,&nbsp;Zhashenjiacan Bao ,&nbsp;Xuesong Chen ,&nbsp;Chuanzhen Wang ,&nbsp;Jiahe Pei ,&nbsp;Zehai Hou ,&nbsp;Lili Ren ,&nbsp;Shixiang Zong ,&nbsp;Jing Tao","doi":"10.1016/j.jtherbio.2025.104180","DOIUrl":"10.1016/j.jtherbio.2025.104180","url":null,"abstract":"<div><div><em>Hylurgus ligniperda</em>, a pine-infesting bark beetle, has successfully invaded Shandong, China, through imported forest timber, posing a significant threat to the country's forestry security. Heat tolerance is a critical survival strategy for insects to endure high summer temperatures, and understanding its mechanisms in <em>H. ligniperda</em> could improve pest forecasting and habitat suitability predictions. In this study, we investigated heat-induced mortality and physiological responses of <em>H. ligniperda</em> under various high-temperature conditions. Our findings revealed that elevated temperatures significantly reduced survival rates of both adults and larvae, with Lt₅₀ (time to 50 % mortality) declining as exposure temperature increased. Notably, <em>H. ligniperda</em> adults exhibited higher heat tolerance than larvae. At 30 °C or 35 °C, water and trehalose content in adults progressively decreased with prolonged exposure (4–12 h). Conversely, lipid, protein, and glycerol levels declined as temperature increased at fixed exposure durations (4, 8, or 12 h). We concluded that short-term extreme heat directly reduced adult survival rates and was associated with depletion of energy reserves (lipid, glycogen, and protein) and dynamic changes in protective molecules (trehalose, sorbitol, and glycerol). This correlation suggests a protective mechanism that mitigates heat damage in <em>H. ligniperda</em>. This study provides foundationalinsights into the heat tolerance strategies and metabolic adaptations of <em>H. ligniperda</em> adults, offering critical data for predicting its spread under climate change scenarios.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"131 ","pages":"Article 104180"},"PeriodicalIF":2.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144581193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hypothermia treatment for ocular ischemia: A multiphase porous thermal model of human eye","authors":"D. Gongal ,&nbsp;J.R. Hetling ,&nbsp;C.D. Foster","doi":"10.1016/j.jtherbio.2025.104139","DOIUrl":"10.1016/j.jtherbio.2025.104139","url":null,"abstract":"<div><div>Localized therapeutic hypothermia is a cooling technique to reduce eye temperature to prevent neuron loss under ischemic conditions. The treatment is a non-invasive intervention technique that aids recovery and may reduce vision loss in ocular ischemia patients. The paper presents a thermal analysis simulation to predict the temperature change in the human eye under local hypothermia treatment using a scleral contact cooling ring device. A thermoregulating blood flow model is introduced to simulate realistic blood circulation in the eye using a three-phase porous media that includes tissue, inflowing blood, and outflowing blood. A transient analysis is performed using a three-dimensional human eye model consisting of an ocular globe and its surrounding fat and muscles. The results show that the optic nerve head and retina temperature decreases to the therapeutic temperature of below 34 °C in 10.58 min of application of the eye cooling device. Sensitivity analysis was performed on all the material and thermal properties to identify the critical variables in the thermal evaluation of the human eye.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"131 ","pages":"Article 104139"},"PeriodicalIF":2.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144581194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heat stress-induced dysregulation of bovine reproduction: A focus on corpus luteum and progesterone perspectives","authors":"Iqra Batool ,&nbsp;Rehana Kausar ,&nbsp;Tarique Hussain","doi":"10.1016/j.jtherbio.2025.104201","DOIUrl":"10.1016/j.jtherbio.2025.104201","url":null,"abstract":"<div><div>Global warming and climate change pose significant challenges to cattle reproduction, with heat stress (HS) emerging as a critical factor contributing to infertility and subfertility in dairy cows. Elevated temperatures in high-producing cattle lead to declines in conception rates and increased incidences of early embryonic loss, thereby undermining the economic sustainability of dairy enterprises. HS induces a cascade of physiological, behavioral, and endocrinological responses, which adversely affect reproductive function, particularly through disruptions in corpus luteum (CL) activity and progesterone (P4) production—both essential for estrous cycle regulation and pregnancy maintenance. Prolonged HS exposure results in suppressed P4 secretion, which compromises the uterine environment necessary for embryo survival. This review examines the mechanisms by which HS impacts CL function and P4 synthesis, contributing to reproductive inefficiencies, and discusses evidence-based mitigation strategies, including environmental modifications, nutritional adjustments, hormonal interventions and genetic selection for thermotolerance. Through these insights, this review aims to highlight effective approaches to enhance fertility, reduce subfertility, and improve reproductive resilience in cattle facing HS-induced stress.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"131 ","pages":"Article 104201"},"PeriodicalIF":2.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144653717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Body mass and mate choice in bumblebees (Bombus terrestris) under climate heating","authors":"Min Su Park ,&nbsp;Ji Hyun Woo ,&nbsp;Hyung Joo Yoon ,&nbsp;Bo Yeon Kim ,&nbsp;Kyeong Yong Lee ,&nbsp;Steven A. Trewick ,&nbsp;Kwang Sik Lee ,&nbsp;Byung Rae Jin","doi":"10.1016/j.jtherbio.2025.104210","DOIUrl":"10.1016/j.jtherbio.2025.104210","url":null,"abstract":"<div><div>Climate warming influences the population size, body size, and diversity of bumblebees, important pollinators in agriculture and ecosystems. Increases in developmental temperatures due to climate warming lead to reduction in bumblebee body size, particularly among the worker caste, resulting in reduced pollination activity. However, how reduced body mass resulting from warm conditions influences mate choice between new queens and males remains unclear. This interaction has a major role in viability of subsequent generations, and we investigated male-male competition and queen mate choice in <em>Bombus terrestris</em> at optimal and elevated mating temperatures (23 °C and 32 °C, respectively). At both temperatures, large males had a significantly higher mating rate than small males. Small males exhibited slightly prolonged mating, while large males transferred more sperm to queens’ spermathecae owing to their higher sperm count. In mating experiments with queens of different body weights, no significant difference was observed in the mating rate between large and small queens at 23 °C, but at 32 °C, large queens had a significantly higher mating rate than small queens, indicating strong male preferences for large queens. Additionally, the survival of mated queens after hibernation was positively correlated with their body weight prior to hibernation. These results demonstrate the changes in the mating preferences of queens and males under warm conditions, suggesting that mate choice shapes subsequent generations in such environments.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"131 ","pages":"Article 104210"},"PeriodicalIF":2.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144623464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}