Journal of Stem Cells & Regenerative Medicine最新文献_第7页

Shimoji's Cranioplasty Yielding Life-quality Improvements in MIld Trigonocephaly (SCYLIMIT). Shimoji颅成形术改善轻度三角头症患者的生活质量。

IF 2.7

Journal of Stem Cells & Regenerative Medicine Pub Date : 2018-12-30 eCollection Date: 2018-01-01

Shimoji Takeyoshi

引用次数: 0

Bone Marrow-derived Osteoblasts in the Management of Ovariectomy induced Osteoporosis in Rats. 骨髓源性成骨细胞治疗大鼠卵巢切除所致骨质疏松症。

IF 2.7

Journal of Stem Cells & Regenerative Medicine Pub Date : 2018-12-30 eCollection Date: 2018-01-01

Mir Sadat-Ali, Haifa A Al-Turki, Sadananda Acharya, Dakheel A Al-Dakheel

{"title":"Bone Marrow-derived Osteoblasts in the Management of Ovariectomy induced Osteoporosis in Rats.","authors":"Mir Sadat-Ali, Haifa A Al-Turki, Sadananda Acharya, Dakheel A Al-Dakheel","doi":"","DOIUrl":"","url":null,"abstract":"Background: The objective of this study is to assess if infusion of osteoblasts can temporarily reverse osteoporosis in rats. Methods: Osteoporosis was induced in 20 female Sprague-Dawley rats by performing ovariectomy (OVX) that was carried out at 4 weeks of age. At 3 months a biopsy of the iliac crest was made to assess the bone quality and the same site bone marrow was harvested. From the bone marrow aspirate, MSCs were separated. Osteoblasts were then generated and were characterized using Alizarin red staining. Osteoblasts were injected in the tail vein of 10 rats. Two weeks after the injection of osteoblasts, a second biopsy was done. Animals were euthanized after 8 weeks of osteoblasts infusion by overdose of ketamine mixed with xylazine. The whole femurs and lumbar spine were dissected and the specimens were stored in 2% formalin. The specimens were analyzed using HRpQCT (High-resolution peripheral quantitative computerized tomography (μCT 100, SCANCO Medical AG, Brüttisellen, Switzerland). Results: In all the 10 animals from which bone aspiration was performed, osteoblasts were cultured and transplanted. Analysis showed that there was significant bone formation at bone sites of distal femur and lumbar spine (<0.001), with increased number of trabeculae and thickness (P<0.001). Further analysis revealed that there was robust bone formation in the animals that had osteoblasts injection. Conclusions: This preliminary study indicates that osteoblasts infusion can lead to new bone formation in osteoporosis induced by ovariectomy in rats.","PeriodicalId":17155,"journal":{"name":"Journal of Stem Cells & Regenerative Medicine","volume":"14 2","pages":"63-68"},"PeriodicalIF":2.7,"publicationDate":"2018-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6339979/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36895526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bone Marrow derived Osteoblasts in the Management of Ovariectomy induced Osteoporosis in Rats 骨髓源性成骨细胞治疗大鼠卵巢切除所致骨质疏松症

IF 2.7

Journal of Stem Cells & Regenerative Medicine Pub Date : 2018-12-30 DOI: 10.46582/jsrm.1402010

引用次数: 1

Induced pluripotent stem cells (iPS) derived disease models: In vivo to in vitro gaps. 诱导多能干细胞（iPS）衍生的疾病模型：体内到体外间隙。

IF 2.7

Journal of Stem Cells & Regenerative Medicine Pub Date : 2018-12-30

引用次数: 0

Germ-line stem cells in myocardial regeneration: Secretion of cardiogenic paracrine effectors may be the future. 生殖系干细胞在心肌再生中的作用:心源性旁分泌效应物的分泌可能是未来的研究方向。

IF 2.7

Journal of Stem Cells & Regenerative Medicine Pub Date : 2018-12-30 eCollection Date: 2018-01-01

G Ian Gallicano

{"title":"Germ-line stem cells in myocardial regeneration: Secretion of cardiogenic paracrine effectors may be the future.","authors":"G Ian Gallicano","doi":"","DOIUrl":"","url":null,"abstract":"Stem cell research for treating or curing ischemic heart disease has, to date, culminated in identifying which scenario is more important; 1) stem cell differentiation into cardiomyocytes that integrate electrically with the heart, 2) stem cells that secrete paracrine factors that promote healing, or 3) a combination of both. We consistently found that unipotent germline stem cells, when removed from their niche and cultured in the correct medium endogenously express pluripotency genes, which induce them to become human germline pluripotent stem cells (hgPSCs). These cells are then capable of producing cell types from all three germ layers. Using hgPSCs along with a modified version of a relatively novel cell-expansion culture methodology to induce quick, indefinite expansion of normally slow growing hgPSCs, it was possible to test the potential of cardiomyocytes derived from hgPSCs for treating an ischemic cardiac event. Upon differentiation into cardiac lineages, our data consistently showed that they not only express cardiac genes, but also express cardiac-promoting paracrine factors. Taking these data a step further, we found that hgPSC-derived cardiac cells can integrate into cardiac tissue in vivo. Note, while the work presented here was based on testes-derived hgPSCs, data from other laboratories have shown that ovaries contain very similar types of stem cells that can give rise to hgPSCs. As a result, hgPSCs should be considered a viable option for eventual use in patients, male or female, with ischemic heart disease.","PeriodicalId":17155,"journal":{"name":"Journal of Stem Cells & Regenerative Medicine","volume":"14 2","pages":"87"},"PeriodicalIF":2.7,"publicationDate":"2018-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6339981/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36884997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An insight into pluripotency and cellular aging through glycan analysis. 通过多糖分析了解多能性和细胞衰老。

IF 2.7

Journal of Stem Cells & Regenerative Medicine Pub Date : 2018-12-30 eCollection Date: 2018-01-01

Hirabayashi Jun

{"title":"An insight into pluripotency and cellular aging through glycan analysis.","authors":"Hirabayashi Jun","doi":"","DOIUrl":"","url":null,"abstract":"All kinds of cells from all kinds of organisms (i.e., animals, plants, fungi and bacteria) are covered by a dense layer of glycans. The origin of glycans or carbohydrates is not known[1], however, the above fact implies that they are widely and closely associated with various biological phenomena based on cellular communications, which include development, differentiation, morphogenesis, carcinogenesis, immunity and infection. It is also notable that glycoproteins, one of existing forms of glycans (i.e., glycoconjugates) are generally synthesized in lumen sites of endoplasmic reticulum and the following Golgi apparatus, distinct from cytoplasmic proteins, which are not subjected to glycosylation, a major event of posttranslational modifications. In fact, glycan structures largely depend on a series of (e.g., >200 in human) glycol-genes, which are defined as genes involved in glycan synthesis (e.g., glycosyltransferases, sulfotransferases, nucleotide sugar transporters), of which expressions differ under different conditions. Because expression of each glycol-gene differs in different cell types (e.g., biological origin, tissue) and states (e.g., developmental stage, malignancy), glycans can be a good marker for cell typing (e.g., SSEA-1) and serum diagnosis (e.g., cancer biomarker such as CA19-9). However, glycan preparation as well as its analysis and total understanding are much more difficult compared with other major disciplines like genomics and proteomics. As a result, most of nonglycoscientists tend to hesitate glycomics, i.e., \"glycophobia\". Nevertheless, glycoscience is a very important field of life science, particularly in the future, without which many remaining issues will not be solved. In this plenary lecture, a novel approach to glycan profiling[2] and its applications to biomarker investigation and regenerative medicine[3] will be described.","PeriodicalId":17155,"journal":{"name":"Journal of Stem Cells & Regenerative Medicine","volume":"14 2","pages":"85"},"PeriodicalIF":2.7,"publicationDate":"2018-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6339976/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36884996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intra-arterial Transplantation of Human Umbilical Cord Blood Mononuclear Cells in Sub-acute Ischemic Stroke Increases VEGF Expression in Rats. 人脐血单核细胞动脉内移植治疗亚急性缺血性脑卒中可增加VEGF的表达。

IF 2.7

Journal of Stem Cells & Regenerative Medicine Pub Date : 2018-12-30 eCollection Date: 2018-01-01

Yetty Ramli, Ahmad Sulaiman Alwahdy, Mohammad Kurniawan, Berry Juliandi, Puspita Eka Wuyung

{"title":"Intra-arterial Transplantation of Human Umbilical Cord Blood Mononuclear Cells in Sub-acute Ischemic Stroke Increases VEGF Expression in Rats.","authors":"Yetty Ramli, Ahmad Sulaiman Alwahdy, Mohammad Kurniawan, Berry Juliandi, Puspita Eka Wuyung","doi":"","DOIUrl":"","url":null,"abstract":"Thrombolysis (rt-PA) is the only United States Food and Drug Administration (FDA) approved drug currently available. Unfortunately, its effect has been limited by the narrow therapeutic time window. Human cord blood mononuclear cells (cbMNC) is a promising treatment for ischemic stroke by forming collateral and neo-vascularization where it is one of the important factors that contribute to cell repair. Therefore, evaluation of neo-vascularization in sub-acute stroke may be beneficial for recovery. One group for healthy rat and three groups (n=6 per group) of male wistar rats have undergone permanent middle cerebral artery occlusion (MCAO). Transplantation 1x106 cells/kg of human cbMNC intra-arterially (IA) and intra-venously (IV) were administered after 7 days. Behavioural tests were performed before MCAO, 1 week after MCAO and at 3,9 and 14 days after cbMNC transplantation. Beta III tubulin protein (TUJ1), glial fibrillary acidic protein (GFAP) and vascular endothelial growth factor (VEGF) antibody marker were evaluated. Spontaneous activity of transplanted rats by cbMNC have significantly improved compared to placebo group (p<0.05). Angiogenesis in IA group showed significant difference (P<0.001) when compared to IV and placebo respectively. The existence of neovascularization in the transplanted rats of cbMNC provide hope in accelerating repairment of the neuronal cells and functional outcome.","PeriodicalId":17155,"journal":{"name":"Journal of Stem Cells & Regenerative Medicine","volume":"14 2","pages":"69-79"},"PeriodicalIF":2.7,"publicationDate":"2018-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6339975/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36895527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Shimoji's Cranioplasty Yielding Life-quality Improvements in MIld Trigonocephaly (SCYLIMIT) Shimoji颅骨成形术改善脑脊髓畸形患者的生活质量

IF 2.7

Journal of Stem Cells & Regenerative Medicine Pub Date : 2018-12-30 DOI: 10.46582/jsrm.1402015

Shimoji Takeyoshi

引用次数: 1

Letter: Safety and Feasibility of Autologous Mesenchymal Stem Cell Transplantation in Chronic Stroke in Indian patients. A four-year follow up. 信:自体间充质干细胞移植治疗印度慢性脑卒中患者的安全性和可行性。四年的随访。

IF 2.7

Journal of Stem Cells & Regenerative Medicine Pub Date : 2018-05-30 eCollection Date: 2018-01-01

Oliver Davis

引用次数: 0

Cardiomyogenic Heterogeneity of Clonal Subpopulations of Human Bone Marrow Mesenchymal Stem Cells. 人骨髓间充质干细胞克隆亚群的心肌形成异质性。

IF 2.7

Journal of Stem Cells & Regenerative Medicine Pub Date : 2018-05-30 eCollection Date: 2018-01-01

Naresh Kumar Tripathy, Syed Husain Mustafa Rizvi, Saurabh Pratap Singh, Venkata Naga Srikanth Garikpati, Soniya Nityanand

{"title":"Cardiomyogenic Heterogeneity of Clonal Subpopulations of Human Bone Marrow Mesenchymal Stem Cells.","authors":"Naresh Kumar Tripathy, Syed Husain Mustafa Rizvi, Saurabh Pratap Singh, Venkata Naga Srikanth Garikpati, Soniya Nityanand","doi":"","DOIUrl":"","url":null,"abstract":"We have evaluated the cardiomyogenic potential of clonal populations of human bone marrow mesenchymal stem cells (BM-MSC). Four rapidly proliferating clones of BM-MSC were obtained from the BM of a healthy donor which were then treated with 5-azacytidine and evaluated for the expression of GATA-4, NKx-2.5, FOG-2, TDGF-1, β-MHC, MEF2D and NPPA genes and cTnT, Desmin and β-MHC proteins. Of the four clones (i) Clone-1 had high expression of GATA-4 (1.89 fold (p<0.05), Nkx2.5 (2.29 fold; p<0.05), FOG2 (2.76 fold; p<0.05), TDGF1 (6.97 fold, p<0.005), βMHC (10.22 fold; p<0.005), MEF-2D (1.91 fold; p<0.005) and NPPA (1.65 fold; p<0.005); (ii) clone-2 had up-regulation of Nkx2.5 (1.98 fold; p<0.05) but down-regulation of rest of the genes; (iii) clone-3 had up-regulation of Nkx2.5 (2.11 fold; p<0.05), TDGF1 (1.88 fold; p<0.05), MEF-2D (1.30 fold; p<0.05) and NPPA (1.21 fold; p<0.05), down regulation of GATA-4 and Fog-2 but no change in βMHC gene; and (iv) clone-4 had up-regulation of MEF-2D (1.17 fold; p<0.05) and down regulation of GATA-4, Nkx2.5 but no change in other genes compared to untreated cells of the clones. At the protein level, clone-1 expressed cTnT, Desmin, and βMHC; clone-2 Desmin only while clones-3 and 4 each expressed cTnT, Desmin, and βMHC. Our data shows that BM-MSC are a heterogenous population of stem cells with sub-populations exhibiting a marked difference in the expression of cardiac markers both at gene and protein levels. This highlights that administering selected sub-populations of BM-MSC with a cardiomyogenic potential may be more efficacious than whole population of cells for cardiac regeneration.","PeriodicalId":17155,"journal":{"name":"Journal of Stem Cells & Regenerative Medicine","volume":"14 1","pages":"27-33"},"PeriodicalIF":2.7,"publicationDate":"2018-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6043656/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36322504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0