Journal of Stem Cells & Regenerative Medicine最新文献

{"title":"Proteomic Comparison of Conditioned Media from Mesenchymal Stem Cells of Different Human Tissues: Implications for Therapy.","authors":"Atsushi Terunuma, Chun Kai Chiu, Tatsuya Sekine, Tsubasa Takane, Yoshihisa Sekine, Keisuke Ashiba, Hiroshi Terunuma","doi":"10.46582/jsrm.2101005","DOIUrl":"10.46582/jsrm.2101005","url":null,"abstract":"<p><strong>Objective: </strong>Mesenchymal stem cells (MSCs) suppress inflammation and promote tissue repair via paracrine factors. MSC-conditioned medium (MSC-CM), rich in these factors, shows promise as a cell-free therapy. This study explored the protein profiles of MSC-CMs from different human tissues (dental pulp, adipose tissue, umbilical cord, and placenta) to assess variations and therapeutic potential.</p><p><strong>Methods: </strong>MSCs were cultured from dental pulp, adipose tissue, umbilical cord, and placenta, and MSC-CMs were collected. Proteomic analysis using LC-MS/MS identified and quantified proteins, followed by bioinformatic analysis.</p><p><strong>Results: </strong>A total of 924 secreted proteins were identified in MSC-CMs from the four tissue sources. Extracellular matrix (ECM) signatures were prominent across all MSC-CMs. MSC-CM from adipose tissue had the highest levels of skin care-related proteins. Neuronal growth-related proteins were most abundant in umbilical cord and placental MSC-CMs, while wound healing proteins were prominent in dental pulp MSC-CM.</p><p><strong>Conclusions: </strong>MSC-CMs from different tissues exhibit distinct protein profiles, while sharing common ECM signatures. These findings suggest that MSC-CM could be used for specific applications such as neurodegenerative diseases and wound healing, depending on the tissue source. Further in vivo research is needed to explore their clinical relevance.</p>","PeriodicalId":17155,"journal":{"name":"Journal of Stem Cells & Regenerative Medicine","volume":"21 1","pages":"25-30"},"PeriodicalIF":1.6,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12311324/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144775692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unique Challenges in myocardial regeneration and the hidden potentials of resident cardiac macrophages.","authors":"Samuel Jk Abraham","doi":"10.46582/jsrm.2101001","DOIUrl":"10.46582/jsrm.2101001","url":null,"abstract":"","PeriodicalId":17155,"journal":{"name":"Journal of Stem Cells & Regenerative Medicine","volume":"21 1","pages":"1-2"},"PeriodicalIF":1.6,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12311325/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144775693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative study the effect of human bone marrow mesenchymal stem cells and cardiomyocytes derived exosomes on improvement of Isoproterenol induced ischemic damaged cardiomyocytes.","authors":"Saeideh Edalati, Pouyan Asadi, Mehdi Sheikh Arabi, Safoura Khajeniazi","doi":"10.46582/jsrm.2101004","DOIUrl":"10.46582/jsrm.2101004","url":null,"abstract":"<p><strong>Background: </strong>Exosomes are small vesicles with intracellular origin which are released into the extracellular space. The properties of stem cell-derived exosomes are similar to their cellular origin and can be involved in repair the damaged tissues. This investigation aimed to evaluate the effect of exosomes derived from mesenchymal stem cells and cardiomyocytes on the repair of damaged cardiomyocyte in vitro.</p><p><strong>Methods: </strong>In this study, first damaged cells were created by Isopreternol treatment of mesenchymal-derived cardiomyocytes, then cells were affected by exosomes extracted from MSCs and MSC derived cardiomyocyes. Finally, mRNA levels of cardiac differentiation and damage markers were measured by Real Time PCR at the time intervals.</p><p><strong>Results: </strong>Our results showed the level of cardiac markers in damaged cardiomyocytes increased after treatment by cardiomyocytes derived exosomes compared to MSC derived exosomes. Reciprocally LDH a and b mRNA levels decrease in both conditions.</p><p><strong>Conclusion: </strong>our findings revealed the exosomes extracted from cardiomyocytes were more effective in the repair of injured cells compared to the exosomes derived MSCs.</p>","PeriodicalId":17155,"journal":{"name":"Journal of Stem Cells & Regenerative Medicine","volume":"21 1","pages":"19-24"},"PeriodicalIF":1.6,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12311328/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144775691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Analysis of the Immunomodulatory Functions of Dental Follicle Stem Cells and Their Apoptotic Vesicles.","authors":"Jie Chen, Tao Lin, Yue Yuan, Peilin Wu, Shishi Dai, Huan Xu, Jingyi Zhang, Jing Ma","doi":"10.46582/jsrm.2101003","DOIUrl":"10.46582/jsrm.2101003","url":null,"abstract":"<p><p>The significant immunomodulatory capacity of mesenchymal stem cells (MSCs) is increasingly being recognized, making them valuable for the treatment of autoimmune disorders. MSCs influence immune cell behavior during therapy through intercellular communication mediated by extracellular vesicles (EVs). Moreover, MSC-derived apoptotic vesicles (apoEVs) can also exert immunomodulatory functions. This study compared the effects of dental follicle stem cells (DFSCs) and smaller apoptotic vesicles (apoSEVs) derived from DFSCs on the proliferation of peripheral blood mononuclear cells (PBMC), as well as their impact on T cell subpopulations and inflammatory factor expression. The results showed that apoSEVs derived from DFSCs significantly enhanced PBMC proliferation, inhibited Th1, Th17, and Treg cell populations, and reduced IFN-γ and TNF-α expression levels. These findings demonstrate that apoSEVsapoSEVs from DFSCs can effectively regulate immune responses in a manner similar to that of DFSCs themselves.</p>","PeriodicalId":17155,"journal":{"name":"Journal of Stem Cells & Regenerative Medicine","volume":"21 1","pages":"11-18"},"PeriodicalIF":1.6,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12311327/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144775690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of autologous stem cells in the treatment of ischaemic cardiomyopathy with heart failure after myocardial infarction.","authors":"Baglan Kemelbekov, Ainur Amanzholkyzy, Samat Saparbayev, Edgaras Stankevicius","doi":"10.46582/jsrm.2101002","DOIUrl":"10.46582/jsrm.2101002","url":null,"abstract":"<p><p>The proposed topic is important because it helps find a lot of problems that happen when cardiovascular diseases are passed on along with fibrosis and the link between stem cells and myocardial regeneration. This study aims to investigate the effectiveness of autologous stem cells in the treatment of post-infarction myocardial changes. Statistical, bibliographic, and bibliosemantic research methods and scientific literature for the last 6 years were used to achieve the purpose. Cardiovascular diseases hold the highest prevalence and mortality rates, second only to the number of accidents. Today, there are many methods in the fight against coronary heart disease. However, drug therapy is the least effective, and instrumental methods are too invasive and entail several complications and side effects. Therefore, conducting detailed research on the impact of stem cells on the myocardium affected by infarction presents a challenge. Stem cell transplantation, which includes autologous bone marrow stem cells, typically leads to noticeable and significant changes in cardiac hemodynamic parameters and rheological properties. The development of autologous bone marrow stem cells during the angiogenesis process substantiates such metamorphoses. In addition, factors such as vascular endothelial growth factor and the whole list of coagulogram indicators may influence these changes. The practical significance of the raised subject is using stem cell therapy as an alternative, less invasive method in the fight against postinfarction myocardial changes.</p>","PeriodicalId":17155,"journal":{"name":"Journal of Stem Cells & Regenerative Medicine","volume":"21 1","pages":"3-10"},"PeriodicalIF":1.6,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12311326/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144775689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Critical criteria for improvising the efficacy of transplanted cells; Significance of route of administration and microenvironment at the target site.","authors":"","doi":"10.46582/jsrm.2002004","DOIUrl":"10.46582/jsrm.2002004","url":null,"abstract":"","PeriodicalId":17155,"journal":{"name":"Journal of Stem Cells & Regenerative Medicine","volume":"20 2","pages":"24-25"},"PeriodicalIF":1.1,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11750065/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143029048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"I. IDC Key-note Lecture: Influence of gut microbiome in Duchenne muscular dystrophy.","authors":"Fabio Arturo Iannotti","doi":"10.46582/jsrm.2002008","DOIUrl":"10.46582/jsrm.2002008","url":null,"abstract":"","PeriodicalId":17155,"journal":{"name":"Journal of Stem Cells & Regenerative Medicine","volume":"20 2","pages":"56-57"},"PeriodicalIF":1.1,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11750062/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143029050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"(PASRM)-2024: I. Stem cell therapies for the cornea.","authors":"Sajjad Ahmad","doi":"10.46582/jsrm.2002007","DOIUrl":"10.46582/jsrm.2002007","url":null,"abstract":"","PeriodicalId":17155,"journal":{"name":"Journal of Stem Cells & Regenerative Medicine","volume":"20 2","pages":"53-55"},"PeriodicalIF":1.1,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11750063/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143029046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Human Adipose Stem Cell-Conditioned Medium (hASC-CM) in Photoaged Skin.","authors":"Winawati Eka Putri, Meidyta Sinantryana Widyaswari, Cita Rosita Sigit Prakoeswa, David Sajid Muhammad, Deny Febriwijaya Romadhani, Nadia Nisaussholihah","doi":"10.46582/jsrm.2002006","DOIUrl":"10.46582/jsrm.2002006","url":null,"abstract":"<p><p><b>Background:</b> Ultraviolet (UV) exposure causes direct and indirect damages to skin structures. Human adipose stem cell-conditioned medium (hASC-CM) is a collection of several soluble factors, such as cytokines, chemokines, and Growth Factors (GF), secreted by almost all living cells in the extracellular space which support wound healing and skin rejuvenation. <b>Objective:</b> To determine the effects of human adipose stem cell-conditioned medium (hASC-CM) in photoaged skin and evaluate photoaging improvement after treatment. <b>Methods:</b> An experimental randomized controlled trial was performed, involving 64 photoaged subjects at the Dermato-Venereology Outpatient Clinic of Jemursari Islamic Hospital Surabaya from March to June 2022. The subjects were divided into the hASC-CM group and vehiculum (control) group. Patients' transepidermal water loss value, skin tone, and Glogau score in weeks 0, 4, and 8 were evaluated. The data were then analyzed using Mann-Whitney test (p<0.05). <b>Results:</b> All subjects had Glogau scale of III (89.6%) with mean ± SD (3.121 ± 0.329). hASC-CM group showed higher improvements in the pore, wrinkle, spot polarized, spot UV parameters and skin tone compared to vehiculum group(p<0.05). <b>Conclusions:</b> hASC-CM effectively improved all parameters observed. The limitation of this research was on the lack of long-term follow-up after treatment. This research had received an ethical permit from The Ethical Committee Board of Jemursari Islamic Hospital Surabaya under letter 006/KEPK-RSISJS/II/2022.</p>","PeriodicalId":17155,"journal":{"name":"Journal of Stem Cells & Regenerative Medicine","volume":"20 2","pages":"47-52"},"PeriodicalIF":1.1,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11750066/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143023936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic Potential of Different Injection Methods for Bone Marrow Mesenchymal Stem Cell Transplantation in Buslfan-Induced Male Rat Infertility.","authors":"Samar Abdelbaset, Mohamed Ahmed Mohamed Sob, Ghada Mutawa, Mai Alaa El-Dein, Amoura Mohamed Abou-El-Naga","doi":"10.46582/jsrm.2002005","DOIUrl":"10.46582/jsrm.2002005","url":null,"abstract":"<p><p><b>Background:</b> In recent years, bone marrow derived mesenchymal stem cells (BM-derived MSCs) have emerged as a powerful cell-based therapy for various diseases, including male infertility. <b>Aim:</b> Demonstrating the efficiency of BM-derived MSCs transplantation by different routes of injection to home and repair testis of busulfan-induced azoospermic rats. <b>Material and methods:</b> In the present study, rat BM-derived MSC was isolated and characterized for mesenchymal &hematopoietic markers using flow-cytometry. Induction of infertility was induced by two successive doses of 10 mg/kg of busulfan. Azoospermic rats were treated by BM-derived MSCs which were injected via various routes (IP, IV, and local in testis). After 60 days; sperm analyses were performed beside mainly Biochemical, histopathological, immunohistological, and ultrastructural investigations. <b>Results:</b> BM-derived MSCs were expressed by CD44+ve, CD105+ve, CD106+ve, CD73+ve, CD34-ve, and CD45-ve. Sperm analysis showed a substantial improvement in sperm morphology, motility, and count following treatment with BM-derived MSCs. Caspase-3 and PCNA immunoexp ression accompanied with the levels of FSH, LH, testosterone, SOD, GSH and MDA depicted a considerable restoration of healthy levels after BM-derived MSCs treatment. The seminiferous tubules showed healthy morphology and spermatozoa were detected in their lumen according to the histopathological and ultrastructural analysis of BM-derived MSCs treated rats. Interestingly, BM-derived MSCs intravenous injection revealed the most significant infertility repair outcomes (P<0.05). <b>Conclusion:</b> Transplanted BM-derived MSCs had the potential to home in rat azoospermic testes and restore spermatogenesis. Consequently, the distinctive characteristics of BM-derived MSCs, such as their ability to differentiate and home, make them a promising cell-based therapeutic option for male infertility.</p>","PeriodicalId":17155,"journal":{"name":"Journal of Stem Cells & Regenerative Medicine","volume":"20 2","pages":"26-46"},"PeriodicalIF":1.1,"publicationDate":"2024-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11750064/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143023938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}