Journal of Stem Cells & Regenerative Medicine最新文献

{"title":"Intraoperative Intramedullary Photobiomodulation Therapy for Consolidation of Atypical Femoral Fractures in Elderly Women: A Pilot Study.","authors":"Daniel Oksman, Gabrielly Santos Pereira, Andre Carvalho Rodriguez, Maria Aparecida Dalboni, Marcelo Lourenco da Silva, Maria Cristina Chavantes","doi":"10.46582/jsrm.2201003","DOIUrl":"10.46582/jsrm.2201003","url":null,"abstract":"<p><strong>Background: </strong>Atypical femoral fractures (AFFs) are rare complications often linked to prolonged bisphosphonate use. This pilot study evaluated the feasibility of intraoperative intramedullary photobiomodulation therapy (PBMT) using long, sterile optical fibers during surgical fixation.</p><p><strong>Methods: </strong>Eleven elderly female patients with AFFs were randomized into a PBMT-treated group or control. PBMT was applied using a red laser (638 nm, 3.2 J per point).</p><p><strong>Results: </strong>No statistically significant differences in healing, complications, or function were observed. However, a trend toward improved RUSTm scores in the PBMT group emerged.</p><p><strong>Conclusion: </strong>This novel technique appears feasible and safe; further studies are needed to assess its regenerative potential.</p>","PeriodicalId":17155,"journal":{"name":"Journal of Stem Cells & Regenerative Medicine","volume":"22 1","pages":"13-21"},"PeriodicalIF":1.3,"publicationDate":"2026-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13214507/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148054734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From Photosynthesis to Photobiomodulation: Expanding the Therapeutic Potential of Light.","authors":"Hescheler Hc Jürgen, Jk Abraham Samuel","doi":"10.46582/jsrm.2201001","DOIUrl":"10.46582/jsrm.2201001","url":null,"abstract":"","PeriodicalId":17155,"journal":{"name":"Journal of Stem Cells & Regenerative Medicine","volume":"22 1","pages":"1-2"},"PeriodicalIF":1.3,"publicationDate":"2026-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13214509/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148143752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Invitro</i> Hepatic like Differentiation of Umbilical Cord-Derived Mesenchymal Stem Cells through FGF and IGF Factor Preconditioning.","authors":"Qureshi Zahid Habib, Maqbool Tahir, Farooq Bakhtawar, Altaf Awais, Azhar Muzammal Mateen, Rafiq Muhammad, Sarwar Muhammad","doi":"10.46582/jsrm.2201004","DOIUrl":"10.46582/jsrm.2201004","url":null,"abstract":"<p><strong>Introduction: </strong>Liver diseases remain a major global health burden, with limited treatment options for advanced hepatic dysfunction. Current treatment options are limited and mainly supportive, with liver transplantation being the only definitive therapy in advanced cases. Stem cell-based therapies have emerged as a promising alternative due to their regenerative and immunomodulatory properties. Stem cells can differentiate and produce identical daughter cells. Umbilical cord-derived mesenchymal stem cells (UC-MSCs) hold promise for liver regeneration but show limited hepatic differentiation efficiency. Preconditioning with FGF and IGF may enhance their differentiation, survival, and functional potential. This study aims to investigate the effect of FGF and IGF pre-treatment on the differentiation capacity of UC-MSCs and their potential application in regenerative therapy for liver fibrosis or cirrhosis.</p><p><strong>Methods: </strong>Cell viability was evaluated through MTT, crystal violet, and trypan blue assays. For the assessment of differentiation potential, ELISA and Immunocytochemistry of HNF4α and ASGPR1 were performed, for angiogenesis and apoptosis, ELISA of VEGF and p53 were performed. Furthermore, antioxidant enzyme activity was also assessed.</p><p><strong>Results: </strong>UC-MSCs preconditioned with FGF and IGF exhibited significantly enhanced viability and reduced cell death, as confirmed by MTT, crystal violet, trypan blue assays, and ELISA of VEGF and P53. ELISA and immunocytochemistry of HNF4α and ASGPR1 demonstrated marked upregulation of these hepatic markers in the preconditioned groups. The gene expression analysis confirmed superior regenerative potential in the FGF+IGF-treated group. Antioxidative analysis further validated a higher level of antioxidative potential in preconditioned cells.</p><p><strong>Conclusion: </strong>Preconditioned UC-MSCs offer a promising cellbased alternative to liver transplantation by enhancing regeneration, reducing apoptosis, promoting angiogenesis and increasing antioxidant defense in damaged liver tissue.</p>","PeriodicalId":17155,"journal":{"name":"Journal of Stem Cells & Regenerative Medicine","volume":"22 1","pages":"22-34"},"PeriodicalIF":1.3,"publicationDate":"2026-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13214508/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148053951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simple Custom Led Panel increases Adipogenic Differentiation of Adipose-Derived Mesenchymal Stem Cells through Photobiomodulation.","authors":"Francisca Ancelia, Isabella Kurnia Liem, Sondang Pandjaitan Sirait","doi":"10.46582/jsrm.2201005","DOIUrl":"10.46582/jsrm.2201005","url":null,"abstract":"<p><strong>Introduction: </strong>Impaired proliferation and differentiation capacity of adipose-derived mesenchymal stem cells (AD-MSCs) in obesity hinder the formation of metabolically healthy adipocytes, thereby aggravating metabolic disorders. Photobiomodulation (PBM) is a simple, non-invasive light-based therapy that primarily uses red and near-infrared light and has been shown to enhance the proliferation and differentiation potential of stem cells, including AD-MSCs. However, the wide variation in PBM parameters used in previous studies necessitates further investigation to determine optimal dosage and delivery methods. This study aimed to construct a simple, easy-to-assemble LED panel for PBM application and to identify an effective dose that stimulates proliferation and adipogenic differentiation of AD-MSCs.</p><p><strong>Methods: </strong>The panel delivered 1 J/cm² of 660 nm red light, either daily (P1Pro and P1Diff group) or every other day (P2Pro and P2Diff group).</p><p><strong>Results: </strong>No significant differences in total cell count or viability were observed among the groups, indicating that the PBM protocol is safe. KI-67 expression was similar between the P1Pro and control groups (Control: 1.00 ± 0.02; P1Pro: 1.19 ± 0.06, p > 0.05), whereas the P2Pro group showed a significant decrease compared with P1Pro (P2Pro: 0.71 ± 0.06, p = 0.02). The P1Diff group also exhibited a significant increase in expression of adipogenic markers PPARꙋ and CEBPα (1.55 ± 0.04, p < 0.01 and 2.9 ± 0.3, p < 0.01, respectively), while the P2Diff group showed minimal to no difference compared with the control.</p><p><strong>Conclusion: </strong>Although further studies are required to assess PBM effects on dysfunctional AD-MSCs from obese individuals, this study demonstrates that a simple, easy-to-assemble PBM device can safely and effectively enhance the proliferation and differentiation capacity of AD-MSCs.</p>","PeriodicalId":17155,"journal":{"name":"Journal of Stem Cells & Regenerative Medicine","volume":"22 1","pages":"35-41"},"PeriodicalIF":1.3,"publicationDate":"2026-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13214511/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148054834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exosomes derived from bone marrow mesenchymal stem cells attenuate OGD/R-induced apoptosis in PC-12 cells via activation of the PI3K/AKT pathway.","authors":"Run-Hao Jiang, Xing-Long Liu, Xin-Xin Huang, Bin Leng, Hai-Bin Shi, Sheng Liu","doi":"10.46582/jsrm.2201002","DOIUrl":"10.46582/jsrm.2201002","url":null,"abstract":"<p><strong>Objective: </strong>This study aims to investigate the role of exosomes derived from bone marrow mesenchymal stem cell (BMSC) with or without hypoxic precondition (HP) play in ischemic stroke, and the underlying mechanisms.</p><p><strong>Methods: </strong>Exosomes were characterized by transmission electron microscopy, western blot, and nanoparticle tracking analysis. To simulate neuronal ischemia-reperfusion injury, PC-12 cells underwent oxygen-glucose deprivation followed by reoxygenation (OGD/R). Cell viability and membrane integrity were assessed using CCK-8 and LDH assays, respectively. Apoptosis was evaluated by flow cytometry and TUNEL assay, while the expression of apoptosis-related proteins was analyzed via western blot.</p><p><strong>Results: </strong>Exosomes derived from BMSC activated the PI3K/Akt signaling pathway and subsequently suppressed apoptosis, thereby ameliorating cellular injury induced by OGD/R. HP enhanced exosome secretion from BMSC; however, it did not significantly alter the particle size distribution of the released exosomes. Notably, when administered at the same concentration, no substantial difference in therapeutic efficacy was observed between exosomes derived from normoxic BMSC and those from HP-treated BMSC. The anti-apoptotic effects conferred by these exosomes were effectively abolished upon treatment with the PI3K inhibitor LY294002, indicating a PI3K/Akt-dependent mechanism.</p><p><strong>Conclusions: </strong>Exosomes derived frvom BMSC effectively inhibitedp aupoptposis induced by OpGD/R through uactivation of the PI3K/Akt. Although HP significantly enhanced the secretion of exosomes from BMSC, it did not improve the therapeutic efficacy of these exosomes when administered at an equivalent dosage.</p>","PeriodicalId":17155,"journal":{"name":"Journal of Stem Cells & Regenerative Medicine","volume":"22 1","pages":"1-10"},"PeriodicalIF":1.3,"publicationDate":"2026-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12887756/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146165834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beyond the scalpel: Remembering Dr K. M. Cherian.","authors":"Jegatheesan Saravana Ganesh, Samuel Jk Abraham","doi":"10.46582/jsrm.2102007","DOIUrl":"10.46582/jsrm.2102007","url":null,"abstract":"","PeriodicalId":17155,"journal":{"name":"Journal of Stem Cells & Regenerative Medicine","volume":"21 2","pages":"32-36"},"PeriodicalIF":1.6,"publicationDate":"2025-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12766064/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145912003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent trend on sarcoma stem cell reprogramming towards study of disease progression: A systematic review.","authors":"Nur Sufiah Zulkifli, Sharaniza Ab-Rahim, Mudiana Muhamad","doi":"10.46582/jsrm.2102010","DOIUrl":"10.46582/jsrm.2102010","url":null,"abstract":"<p><p>Sarcoma refers to a broad type of cancer characterised by the abnormal growth of cells in connective tissues, bone, muscle, and cartilage. Although relatively rare, the survival rate of sarcoma patients is low primarily due to delayed diagnosis or metastasis at first presentation. Highly metastatic sarcoma has been suggested to be attributable to the presence of cancer stem cells (CSCs). Although a minor cell population, the stemness property renders CSCs stem cell-like characteristics. Nevertheless, CSCs presented a promising target towards advances in sarcoma therapy, particularly through stem cell reprogramming. Thus, this systematic review aims to gather existing studies on the methods of stem cell reprogramming that best produce sarcoma cancer stem cells (CSCs), which are crucial for understanding disease progression. An extensive literature search was conducted across four databases: PubMed, Wiley Online Library, Scopus, and ScienceDirect. The data obtained were synthesized and reported according to the following variables: types of cancer cells used for cancer stem cell generation, vector used for delivery of pluripotent genes, and CSCs maintenance medium. This systematic review demonstrated that cell dedifferentiation was independent of the cell sources. Furthermore, the addition of growth factors such as bFGF, EGF, or FGF significantly enhanced the formation of CSCs' spheroids. Most of the studies included in the review utilized a non-viral vector for the delivery of pluripotent gene markers into the cells.</p>","PeriodicalId":17155,"journal":{"name":"Journal of Stem Cells & Regenerative Medicine","volume":"21 2","pages":"59-68"},"PeriodicalIF":1.6,"publicationDate":"2025-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12766068/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145912039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"I. Enhanced Autologous Immune Enhancement Therapy (AIET) for Cancer Treatment in Vietnam: From Clinical Trials to Broader.","authors":"Nguyen Thanh Liem","doi":"10.46582/jsrm.2102013","DOIUrl":"10.46582/jsrm.2102013","url":null,"abstract":"","PeriodicalId":17155,"journal":{"name":"Journal of Stem Cells & Regenerative Medicine","volume":"21 2","pages":"80-81"},"PeriodicalIF":1.6,"publicationDate":"2025-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12766066/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145912061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"(PASRM)-2025: I. Buccal epithelium Expanded and Encapsulated in Scaffold-Hybrid Approach to Urethral Stricture (BEES-HAUS) Cell Therapy Technology - Bench to Bedside.","authors":"Akio Horiguchi","doi":"10.46582/jsrm.2102012","DOIUrl":"10.46582/jsrm.2102012","url":null,"abstract":"","PeriodicalId":17155,"journal":{"name":"Journal of Stem Cells & Regenerative Medicine","volume":"21 2","pages":"78-79"},"PeriodicalIF":1.6,"publicationDate":"2025-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12766065/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145912073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of Small Molecular Drug-Loaded Microspheres for Enhanced Cartilage Regeneration.","authors":"Hsiang-Chien Tseng, Chih-Pei Hsu, Hung-Sheng Soung, Yu-Chuan Lin, Mao-Hsien Wang","doi":"10.46582/jsrm.2102011","DOIUrl":"10.46582/jsrm.2102011","url":null,"abstract":"<p><p>Articular cartilage has limited self-repair ability, making osteoarthritis a major challenge due to aging and mechanical stress. While current therapies provide partial functional improvement, their outcomes remain suboptimal, highlighting the need for regenerative strategies. Mesenchymal stem cells (MSCs) show promises due to their chondrogenic potential, and kartogenin (KGN) enhances this process. This study examines KGN-loaded gelatin microspheres (KMs) for cartilage regeneration. KMs were synthesized via gelatin-KGN dispersion in ice-cooled hexane, followed by glutaraldehyde cross-linking. Scanning electron microscopy showed porous KMs (200-800 μm) with interconnected pores (2-10 μm), while Fourier transform infrared spectroscopy confirmed gelatin and KGN presence. hMSCs were cultured with KMs, showing no cytotoxicity. Gene expression analysis revealed upregulated chondrogenic markers (SOX9, ACAN, COMP, COL2A1). Western blotting and immunofluorescence confirmed increased chondrogenic protein production. Sulfated glycosaminoglycan content increased over four weeks, indicating extracellular matrix maturation. This study demonstrates that KMs effectively deliver KGN, enhancing MSC chondrogenesis. Small molecule-based biomaterials may offer an alternative to growth factors in osteochondral tissue engineering, warranting further in vivo validation.</p>","PeriodicalId":17155,"journal":{"name":"Journal of Stem Cells & Regenerative Medicine","volume":"21 2","pages":"69-77"},"PeriodicalIF":1.6,"publicationDate":"2025-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12766069/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145911981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}