Journal of Stem Cells & Regenerative Medicine最新文献

Exosomes derived from bone marrow mesenchymal stem cells attenuate OGD/R-induced apoptosis in PC-12 cells via activation of the PI3K/AKT pathway. 来自骨髓间充质干细胞的外泌体通过激活PI3K/AKT通路减弱OGD/ r诱导的PC-12细胞凋亡。

IF 1.6

Journal of Stem Cells & Regenerative Medicine Pub Date : 2026-02-04 eCollection Date: 2025-01-01 DOI: 10.46582/jsrm.2201002

Run-Hao Jiang, Xing-Long Liu, Xin-Xin Huang, Bin Leng, Hai-Bin Shi, Sheng Liu

{"title":"Exosomes derived from bone marrow mesenchymal stem cells attenuate OGD/R-induced apoptosis in PC-12 cells via activation of the PI3K/AKT pathway.","authors":"Run-Hao Jiang, Xing-Long Liu, Xin-Xin Huang, Bin Leng, Hai-Bin Shi, Sheng Liu","doi":"10.46582/jsrm.2201002","DOIUrl":"10.46582/jsrm.2201002","url":null,"abstract":"Objective: This study aims to investigate the role of exosomes derived from bone marrow mesenchymal stem cell (BMSC) with or without hypoxic precondition (HP) play in ischemic stroke, and the underlying mechanisms.Methods: Exosomes were characterized by transmission electron microscopy, western blot, and nanoparticle tracking analysis. To simulate neuronal ischemia-reperfusion injury, PC-12 cells underwent oxygen-glucose deprivation followed by reoxygenation (OGD/R). Cell viability and membrane integrity were assessed using CCK-8 and LDH assays, respectively. Apoptosis was evaluated by flow cytometry and TUNEL assay, while the expression of apoptosis-related proteins was analyzed via western blot.Results: Exosomes derived from BMSC activated the PI3K/Akt signaling pathway and subsequently suppressed apoptosis, thereby ameliorating cellular injury induced by OGD/R. HP enhanced exosome secretion from BMSC; however, it did not significantly alter the particle size distribution of the released exosomes. Notably, when administered at the same concentration, no substantial difference in therapeutic efficacy was observed between exosomes derived from normoxic BMSC and those from HP-treated BMSC. The anti-apoptotic effects conferred by these exosomes were effectively abolished upon treatment with the PI3K inhibitor LY294002, indicating a PI3K/Akt-dependent mechanism.Conclusions: Exosomes derived frvom BMSC effectively inhibitedp aupoptposis induced by OpGD/R through uactivation of the PI3K/Akt. Although HP significantly enhanced the secretion of exosomes from BMSC, it did not improve the therapeutic efficacy of these exosomes when administered at an equivalent dosage.","PeriodicalId":17155,"journal":{"name":"Journal of Stem Cells & Regenerative Medicine","volume":"22 1","pages":"1-10"},"PeriodicalIF":1.6,"publicationDate":"2026-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146165834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beyond the scalpel: Remembering Dr K. M. Cherian. 手术刀之外：纪念谢立安医生。

IF 1.6

Journal of Stem Cells & Regenerative Medicine Pub Date : 2025-12-22 eCollection Date: 2025-01-01 DOI: 10.46582/jsrm.2102007

Jegatheesan Saravana Ganesh, Samuel Jk Abraham

引用次数: 0

Recent trend on sarcoma stem cell reprogramming towards study of disease progression: A systematic review. 肉瘤干细胞重编程在疾病进展研究中的最新趋势：系统综述。

IF 1.6

Journal of Stem Cells & Regenerative Medicine Pub Date : 2025-12-22 eCollection Date: 2025-01-01 DOI: 10.46582/jsrm.2102010

Nur Sufiah Zulkifli, Sharaniza Ab-Rahim, Mudiana Muhamad

{"title":"Recent trend on sarcoma stem cell reprogramming towards study of disease progression: A systematic review.","authors":"Nur Sufiah Zulkifli, Sharaniza Ab-Rahim, Mudiana Muhamad","doi":"10.46582/jsrm.2102010","DOIUrl":"10.46582/jsrm.2102010","url":null,"abstract":"Sarcoma refers to a broad type of cancer characterised by the abnormal growth of cells in connective tissues, bone, muscle, and cartilage. Although relatively rare, the survival rate of sarcoma patients is low primarily due to delayed diagnosis or metastasis at first presentation. Highly metastatic sarcoma has been suggested to be attributable to the presence of cancer stem cells (CSCs). Although a minor cell population, the stemness property renders CSCs stem cell-like characteristics. Nevertheless, CSCs presented a promising target towards advances in sarcoma therapy, particularly through stem cell reprogramming. Thus, this systematic review aims to gather existing studies on the methods of stem cell reprogramming that best produce sarcoma cancer stem cells (CSCs), which are crucial for understanding disease progression. An extensive literature search was conducted across four databases: PubMed, Wiley Online Library, Scopus, and ScienceDirect. The data obtained were synthesized and reported according to the following variables: types of cancer cells used for cancer stem cell generation, vector used for delivery of pluripotent genes, and CSCs maintenance medium. This systematic review demonstrated that cell dedifferentiation was independent of the cell sources. Furthermore, the addition of growth factors such as bFGF, EGF, or FGF significantly enhanced the formation of CSCs' spheroids. Most of the studies included in the review utilized a non-viral vector for the delivery of pluripotent gene markers into the cells.","PeriodicalId":17155,"journal":{"name":"Journal of Stem Cells & Regenerative Medicine","volume":"21 2","pages":"59-68"},"PeriodicalIF":1.6,"publicationDate":"2025-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12766068/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145912039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

I. Enhanced Autologous Immune Enhancement Therapy (AIET) for Cancer Treatment in Vietnam: From Clinical Trials to Broader. 1 .增强自身免疫增强疗法（AIET）在越南的癌症治疗：从临床试验到更广泛的研究。

IF 1.6

Journal of Stem Cells & Regenerative Medicine Pub Date : 2025-12-22 eCollection Date: 2025-01-01 DOI: 10.46582/jsrm.2102013

Nguyen Thanh Liem

引用次数: 0

(PASRM)-2025: I. Buccal epithelium Expanded and Encapsulated in Scaffold-Hybrid Approach to Urethral Stricture (BEES-HAUS) Cell Therapy Technology - Bench to Bedside. （PASRM）-2025: 1 .尿道狭窄支架-混合入路（BEES-HAUS）细胞治疗技术-从实验室到床边的扩展和包被颊上皮。

IF 1.6

Journal of Stem Cells & Regenerative Medicine Pub Date : 2025-12-22 eCollection Date: 2025-01-01 DOI: 10.46582/jsrm.2102012

Akio Horiguchi

引用次数: 0

Development of Small Molecular Drug-Loaded Microspheres for Enhanced Cartilage Regeneration. 用于软骨再生的小分子载药微球的研制。

IF 1.6

Journal of Stem Cells & Regenerative Medicine Pub Date : 2025-12-22 eCollection Date: 2025-01-01 DOI: 10.46582/jsrm.2102011

Hsiang-Chien Tseng, Chih-Pei Hsu, Hung-Sheng Soung, Yu-Chuan Lin, Mao-Hsien Wang

{"title":"Development of Small Molecular Drug-Loaded Microspheres for Enhanced Cartilage Regeneration.","authors":"Hsiang-Chien Tseng, Chih-Pei Hsu, Hung-Sheng Soung, Yu-Chuan Lin, Mao-Hsien Wang","doi":"10.46582/jsrm.2102011","DOIUrl":"10.46582/jsrm.2102011","url":null,"abstract":"Articular cartilage has limited self-repair ability, making osteoarthritis a major challenge due to aging and mechanical stress. While current therapies provide partial functional improvement, their outcomes remain suboptimal, highlighting the need for regenerative strategies. Mesenchymal stem cells (MSCs) show promises due to their chondrogenic potential, and kartogenin (KGN) enhances this process. This study examines KGN-loaded gelatin microspheres (KMs) for cartilage regeneration. KMs were synthesized via gelatin-KGN dispersion in ice-cooled hexane, followed by glutaraldehyde cross-linking. Scanning electron microscopy showed porous KMs (200-800 μm) with interconnected pores (2-10 μm), while Fourier transform infrared spectroscopy confirmed gelatin and KGN presence. hMSCs were cultured with KMs, showing no cytotoxicity. Gene expression analysis revealed upregulated chondrogenic markers (SOX9, ACAN, COMP, COL2A1). Western blotting and immunofluorescence confirmed increased chondrogenic protein production. Sulfated glycosaminoglycan content increased over four weeks, indicating extracellular matrix maturation. This study demonstrates that KMs effectively deliver KGN, enhancing MSC chondrogenesis. Small molecule-based biomaterials may offer an alternative to growth factors in osteochondral tissue engineering, warranting further in vivo validation.","PeriodicalId":17155,"journal":{"name":"Journal of Stem Cells & Regenerative Medicine","volume":"21 2","pages":"69-77"},"PeriodicalIF":1.6,"publicationDate":"2025-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12766069/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145911981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

INVESTIGATING THE EFFECT OF MESENCHYMAL STEM CELLS ON THE PROLIFERATION PHASE OF DUODENAL WOUND HEALING. 探讨间充质干细胞对十二指肠创面增殖期愈合的影响。

IF 1.6

Journal of Stem Cells & Regenerative Medicine Pub Date : 2025-12-22 eCollection Date: 2025-01-01 DOI: 10.46582/jsrm.2102008

Eko Setiawan, Dimas Irfan Nabih

{"title":"INVESTIGATING THE EFFECT OF MESENCHYMAL STEM CELLS ON THE PROLIFERATION PHASE OF DUODENAL WOUND HEALING.","authors":"Eko Setiawan, Dimas Irfan Nabih","doi":"10.46582/jsrm.2102008","DOIUrl":"10.46582/jsrm.2102008","url":null,"abstract":"Background: Duodenal perforation represents a critical gastrointestinal surgical emergency, often associated with high rates of morbidity and mortality. Mesenchymal stem cells (MSCs) have the potential to enhance wound healing by releasing various growth factors and antiinflammatory cytokines. This study aims to evaluate and analyze the impact of MSCs on the healing process of duodenal perforation wounds.Method: MSCs were extracted from the umbilical cords of rats and injected into the site of duodenal perforation at two different doses: 1.5 × 106 for Treatment Group 1 and 3 × 106 for Treatment Group 2. The control group consisted of rats with duodenal perforation that received local injections on normal saline. The levels of Transforming Growth Factor β (TGF-β) and Alpha Smooth Muscle Actin (α-SMA) were assessed via Western Blot analysis, while collagen and fibroblast presence were evaluated through histopathological examination. These examinations were conducted on days 3 and 7 post-treatment. Statistical analysis was performed using SPSS 25.0, with significance defined at p<0.05.Results: Significant increases in the expression of TGF-β, fibroblasts, collagen, and α-SMA were observed in the treatment groups compared to the control group on both day 3 and day 7.Conclusion: The administration of MSCs significantly enhances the proliferation phase of duodenal wound healing through the increased expression of fibroblasts, collagen, TGF-β, and α-SMA.","PeriodicalId":17155,"journal":{"name":"Journal of Stem Cells & Regenerative Medicine","volume":"21 2","pages":"37-48"},"PeriodicalIF":1.6,"publicationDate":"2025-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12766067/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145912015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Editorial Commentary: Toward Off-the-Shelf Ureteral Substitutes Using iBTA "Biotubes". 编辑评论：使用iBTA“生物管”的现成输尿管替代品。

IF 1.6

Journal of Stem Cells & Regenerative Medicine Pub Date : 2025-12-22 eCollection Date: 2025-01-01 DOI: 10.46582/jsrm.2102006

Akio Horiguchi

引用次数: 0

Ureter reconstruction using a biotube in a canine model: A pilot stud. 用生物管在犬模型中重建输尿管：一种试验种马。

IF 1.6

Journal of Stem Cells & Regenerative Medicine Pub Date : 2025-12-22 eCollection Date: 2025-01-01 DOI: 10.46582/jsrm.2102009

Noritaka Maeta, Rosuke Iwai, Yohei Mochizuki, Natsuki Akashi, Masahiro Miyabe, Akira Matsuda, Kenji Kutara, Keisuke Sugimoto, Takamasa Itoi, Yasuhide Nakayama

{"title":"Ureter reconstruction using a biotube in a canine model: A pilot stud.","authors":"Noritaka Maeta, Rosuke Iwai, Yohei Mochizuki, Natsuki Akashi, Masahiro Miyabe, Akira Matsuda, Kenji Kutara, Keisuke Sugimoto, Takamasa Itoi, Yasuhide Nakayama","doi":"10.46582/jsrm.2102009","DOIUrl":"10.46582/jsrm.2102009","url":null,"abstract":"Introduction: Ureteral injuries can lead to renal dysfunction, and conventional treatments such as ureteral stents and autologous tissue grafts have limitations. This study aimed to evaluate the biocompatibility of a \"biotube\" created using in-body tissue architecture (iBTA) technology for ureteral reconstruction in a canine model.Methods: Biotubes were implanted in dogs to replace a segment of the ureter. Ureteral stents were used to prevent obstruction. Autopsies were performed 2-3 months after implantation, and the implantation sites were examined histologically to assess tissue regeneration, vascularization, and potential inflammatory reactions.Results: Ureteral stenting effectively prevented obstruction and ensured adequate urinary flow during the observation period. All biotubes were significantly shortened after implantation. No signs of inflammation or foreign body reactions were recorded, indicating good biocompatibility. Although epithelial cell invasion was observed, muscle tissue migration and angiogenesis were limited.Conclusions: Biotubes demonstrate potential as temporary ureteral substitutes; however, improvements in epithelialization and muscle tissue migration are necessary for successful long-term ureteral regeneration. Further experimental studies are required to evaluate their clinical utility as scaffold materials for ureteral reconstruction.","PeriodicalId":17155,"journal":{"name":"Journal of Stem Cells & Regenerative Medicine","volume":"21 2","pages":"49-58"},"PeriodicalIF":1.6,"publicationDate":"2025-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12766063/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145912072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteomic Comparison of Conditioned Media from Mesenchymal Stem Cells of Different Human Tissues: Implications for Therapy. 不同人体组织间充质干细胞条件培养基的蛋白质组学比较：对治疗的意义。

IF 1.6

Journal of Stem Cells & Regenerative Medicine Pub Date : 2025-05-29 eCollection Date: 2025-01-01 DOI: 10.46582/jsrm.2101005

Atsushi Terunuma, Chun Kai Chiu, Tatsuya Sekine, Tsubasa Takane, Yoshihisa Sekine, Keisuke Ashiba, Hiroshi Terunuma

{"title":"Proteomic Comparison of Conditioned Media from Mesenchymal Stem Cells of Different Human Tissues: Implications for Therapy.","authors":"Atsushi Terunuma, Chun Kai Chiu, Tatsuya Sekine, Tsubasa Takane, Yoshihisa Sekine, Keisuke Ashiba, Hiroshi Terunuma","doi":"10.46582/jsrm.2101005","DOIUrl":"10.46582/jsrm.2101005","url":null,"abstract":"Objective: Mesenchymal stem cells (MSCs) suppress inflammation and promote tissue repair via paracrine factors. MSC-conditioned medium (MSC-CM), rich in these factors, shows promise as a cell-free therapy. This study explored the protein profiles of MSC-CMs from different human tissues (dental pulp, adipose tissue, umbilical cord, and placenta) to assess variations and therapeutic potential.Methods: MSCs were cultured from dental pulp, adipose tissue, umbilical cord, and placenta, and MSC-CMs were collected. Proteomic analysis using LC-MS/MS identified and quantified proteins, followed by bioinformatic analysis.Results: A total of 924 secreted proteins were identified in MSC-CMs from the four tissue sources. Extracellular matrix (ECM) signatures were prominent across all MSC-CMs. MSC-CM from adipose tissue had the highest levels of skin care-related proteins. Neuronal growth-related proteins were most abundant in umbilical cord and placental MSC-CMs, while wound healing proteins were prominent in dental pulp MSC-CM.Conclusions: MSC-CMs from different tissues exhibit distinct protein profiles, while sharing common ECM signatures. These findings suggest that MSC-CM could be used for specific applications such as neurodegenerative diseases and wound healing, depending on the tissue source. Further in vivo research is needed to explore their clinical relevance.","PeriodicalId":17155,"journal":{"name":"Journal of Stem Cells & Regenerative Medicine","volume":"21 1","pages":"25-30"},"PeriodicalIF":1.6,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12311324/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144775692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0