Comparative study the effect of human bone marrow mesenchymal stem cells and cardiomyocytes derived exosomes on improvement of Isoproterenol induced ischemic damaged cardiomyocytes.

IF 1.6 Q4 CELL & TISSUE ENGINEERING

Journal of Stem Cells & Regenerative Medicine Pub Date : 2025-05-29 eCollection Date: 2025-01-01 DOI:10.46582/jsrm.2101004

Saeideh Edalati, Pouyan Asadi, Mehdi Sheikh Arabi, Safoura Khajeniazi

{"title":"Comparative study the effect of human bone marrow mesenchymal stem cells and cardiomyocytes derived exosomes on improvement of Isoproterenol induced ischemic damaged cardiomyocytes.","authors":"Saeideh Edalati, Pouyan Asadi, Mehdi Sheikh Arabi, Safoura Khajeniazi","doi":"10.46582/jsrm.2101004","DOIUrl":null,"url":null,"abstract":"Background: Exosomes are small vesicles with intracellular origin which are released into the extracellular space. The properties of stem cell-derived exosomes are similar to their cellular origin and can be involved in repair the damaged tissues. This investigation aimed to evaluate the effect of exosomes derived from mesenchymal stem cells and cardiomyocytes on the repair of damaged cardiomyocyte in vitro.Methods: In this study, first damaged cells were created by Isopreternol treatment of mesenchymal-derived cardiomyocytes, then cells were affected by exosomes extracted from MSCs and MSC derived cardiomyocyes. Finally, mRNA levels of cardiac differentiation and damage markers were measured by Real Time PCR at the time intervals.Results: Our results showed the level of cardiac markers in damaged cardiomyocytes increased after treatment by cardiomyocytes derived exosomes compared to MSC derived exosomes. Reciprocally LDH a and b mRNA levels decrease in both conditions.Conclusion: our findings revealed the exosomes extracted from cardiomyocytes were more effective in the repair of injured cells compared to the exosomes derived MSCs.","PeriodicalId":17155,"journal":{"name":"Journal of Stem Cells & Regenerative Medicine","volume":"21 1","pages":"19-24"},"PeriodicalIF":1.6000,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12311328/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Stem Cells & Regenerative Medicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.46582/jsrm.2101004","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q4","JCRName":"CELL & TISSUE ENGINEERING","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Exosomes are small vesicles with intracellular origin which are released into the extracellular space. The properties of stem cell-derived exosomes are similar to their cellular origin and can be involved in repair the damaged tissues. This investigation aimed to evaluate the effect of exosomes derived from mesenchymal stem cells and cardiomyocytes on the repair of damaged cardiomyocyte in vitro.

Methods: In this study, first damaged cells were created by Isopreternol treatment of mesenchymal-derived cardiomyocytes, then cells were affected by exosomes extracted from MSCs and MSC derived cardiomyocyes. Finally, mRNA levels of cardiac differentiation and damage markers were measured by Real Time PCR at the time intervals.

Results: Our results showed the level of cardiac markers in damaged cardiomyocytes increased after treatment by cardiomyocytes derived exosomes compared to MSC derived exosomes. Reciprocally LDH a and b mRNA levels decrease in both conditions.

Conclusion: our findings revealed the exosomes extracted from cardiomyocytes were more effective in the repair of injured cells compared to the exosomes derived MSCs.

查看原文本刊更多论文

人骨髓间充质干细胞与心肌细胞源性外泌体对异丙肾上腺素诱导的缺血性损伤心肌细胞改善作用的比较研究。

外泌体是细胞内起源的小囊泡，被释放到细胞外空间。干细胞来源的外泌体的特性与其细胞起源相似，可以参与损伤组织的修复。本研究旨在评估间充质干细胞和心肌细胞来源的外泌体在体外修复受损心肌细胞中的作用。方法：在本研究中，首先用异戊二醇处理间充质来源的心肌细胞，形成损伤细胞，然后用从间充质干细胞和间充质来源的心肌细胞中提取的外泌体影响细胞。最后，通过Real Time PCR检测各组心脏分化和损伤标志物的mRNA水平。结果：我们的研究结果显示，与MSC来源的外泌体相比，心肌细胞来源的外泌体治疗后受损心肌细胞中的心脏标志物水平升高。相反，LDH a和b mRNA水平在两种情况下均下降。结论：我们的研究结果表明，与外泌体衍生的间充质干细胞相比，从心肌细胞中提取的外泌体在修复损伤细胞方面更有效。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊