Journal of Surgical Research最新文献

{"title":"Efficient Free Flap Monitoring—A Single-Center Study Comparing Different Monitoring Periods","authors":"David Kampshoff MD ,&nbsp;Charalampos Varnava MD ,&nbsp;Alexander Dermietzel MD ,&nbsp;Philipp Wiebringhaus MD ,&nbsp;Tobias Hirsch MD ,&nbsp;Maximilian Kueckelhaus MD, MBA","doi":"10.1016/j.jss.2024.12.056","DOIUrl":"10.1016/j.jss.2024.12.056","url":null,"abstract":"<div><h3>Introduction</h3><div>Postoperative monitoring is an important part in the complex process of reconstructive microsurgery. While it can improve quality and outcome of microsurgical reconstructions, it is also very resource intensive. The aim of this study was to investigate the effectiveness of postoperative flap monitoring.</div></div><div><h3>Methods</h3><div>In this study, we analyzed outcomes and complications after microsurgical reconstruction. The initial postoperative monitoring period was operation day and the following five postoperative days with clinical evaluation every 2 hs. Following initial evaluation of 124 cases, we modified our postoperative monitoring scheme toward a shorter monitoring period. After performance of further 100 free flaps, outcomes of the two monitoring schemes were analyzed in depth and compared retrospectively.</div></div><div><h3>Results</h3><div>Eighty-nine percent of complications with the need of revision occurred within the operation day and the following 3 ds postoperatively. Total flap loss was 6.5%. Flap salvage rates after revision were 50-80% in this period. Flaps that had a later revision could not be saved (<em>n</em> = 2). The optimized monitoring protocol includes a clinical examination on the day of surgery and for the three postoperative days. In the event of intraoperative complications, the senior surgeon could extend postoperative monitoring to five postoperative days, which was decided for in four cases. After optimizing the protocol, total flap loss was 3%. Flap salvage rates after revision varied between 33% and 100%.</div></div><div><h3>Conclusions</h3><div>Flap monitoring is crucial for maximizing the salvage rate in microsurgical reconstruction. Optimizing our monitoring protocol toward a shorter postoperative monitoring period in our cohort did not lead to an increased complication rate while saving resources.</div></div>","PeriodicalId":17030,"journal":{"name":"Journal of Surgical Research","volume":"307 ","pages":"Pages 1-7"},"PeriodicalIF":1.8,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143177753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preferences for Inguinal Hernia Repair in Infants: A Survey of the Eastern Pediatric Surgery Network","authors":"Zane J. Hellmann MD ,&nbsp;J. Leslie Knod MD ,&nbsp;Afif N. Kulaylat MD ,&nbsp;Cornelia Griggs MD ,&nbsp;Jennifer R. DeFazio MD ,&nbsp;Stefan Scholz MD ,&nbsp;Hanna Alemayehu MD ,&nbsp;Jamie R. Robinson MD, PhD ,&nbsp;Shaun M. Kunisaki MD, MSc ,&nbsp;Matthew A. Hornick MD","doi":"10.1016/j.jss.2024.12.012","DOIUrl":"10.1016/j.jss.2024.12.012","url":null,"abstract":"<div><h3>Introduction</h3><div>Laparoscopic inguinal hernia repair (IHR) is being performed more frequently in children, but few studies have evaluated surgical practice patterns in infants. In this study, we surveyed pediatric surgeons within a regional consortium to assess current preferences for IHR strategy in infants. We hypothesized that early-career pediatric surgeons would prefer laparoscopic IHR over open IHR in this patient population.</div></div><div><h3>Methods</h3><div>A Qualtrics survey addressing surgeon preferences for IHR was distributed to 160 pediatric surgeons at 19 member institutions affiliated with the Eastern Pediatric Surgery Network. Surgeons were stratified by self-reported number of years in attending practice. Responses were compared using <em>t</em>-tests and chi-square tests wherever appropriate.</div></div><div><h3>Results</h3><div>Ninety-eight surgeons responded to the survey (61% response rate; two incomplete responses were excluded). Forty respondents (41.7%) had 0-10 ys of experience, 26 (27.1%) had 10-20 ys of experience, and 30 (31.2%) had over 20 ys of experience. Over 90% of early-career surgeons reported a preference for laparoscopic IHR in infants, compared to less than 50% of mid-career surgeons and less than 20% of late-career surgeons (<em>P</em> &lt; 0.001). Respondents preferring laparoscopic IHR most commonly cited inherent assessment of the contralateral side, confirmation of hernia before repair, and technical ease of the laparoscopic approach as factors contributing to their preference.</div></div><div><h3>Conclusions</h3><div>The majority of early-career pediatric surgeons prefer laparoscopic IHR over open IHR in infants, representing a substantial shift away from what is traditionally regarded as the gold standard open technique. Larger studies are needed to compare long-term outcomes after laparoscopic and open IHR in infants.</div></div>","PeriodicalId":17030,"journal":{"name":"Journal of Surgical Research","volume":"306 ","pages":"Pages 188-196"},"PeriodicalIF":1.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142965586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Disparities in Rehabilitation Services for Victims of Violence","authors":"Megan G. Janeway MD ,&nbsp;Ella Cornell MD ,&nbsp;Sophia M. Smith MD, MS ,&nbsp;Anne K. Buck MS ,&nbsp;Miriam Neufeld MD, MPH ,&nbsp;Janice Weinberg ScD ,&nbsp;Stephanie D. Talutis MD, MPH ,&nbsp;Nina Jreige MD ,&nbsp;Victoria Liang MD ,&nbsp;Timothy Munzert MSW, LICSW ,&nbsp;Tracey Dechert MD, FACS ,&nbsp;Sabrina E. Sanchez MD, MPH ,&nbsp;Lisa Allee MSW, LICSW","doi":"10.1016/j.jss.2024.12.040","DOIUrl":"10.1016/j.jss.2024.12.040","url":null,"abstract":"<div><h3>Introduction</h3><div>Access to rehabilitation services after a traumatic injury improves functional outcomes. No study has examined the association between injury intent, violent <em>versus</em> nonviolent, and receipt of rehabilitation services after injury.</div></div><div><h3>Materials and methods</h3><div>We conducted a retrospective cohort study of injured adult patients admitted to our level I trauma center from January 1, 2014 to December 31, 2021. The primary exposure was violent injury, and the primary outcome was receipt of rehabilitation services upon discharge. An exploratory subgroup analysis evaluated differences in recommended disposition and the reasons for rejection from services.</div></div><div><h3>Results</h3><div>Among 7500 patients, 1677 (22.4%) were violently injured and 5823 (77.6%) were nonviolently injured. Patients were 45% White, 67% male, and 52% had public insurance. Adjusting for age, sex, race, ethnicity, injury severity score, insurance, and length of stay, violently injured patients were 77% less likely to receive inpatient rehabilitation (relative risk ratio 0.23 95% confidence interval [0.18, 0.30], <em>P</em> &lt; 0.001) and 46% less likely to have home services (relative risk ratio 0.54, 95% confidence interval[0.43, 0.69], <em>P</em> &lt; 0.001). A subgroup analysis (<em>n</em> = 328) demonstrated that violently injured patients were more likely to have a downgrade in discharge recommendation (27.8% <em>versus</em> 9.4%, <em>P</em> = 0.04) and more likely to have an emergency department visit within 30 d (32.0% <em>versus</em> 13.3%, <em>P</em> &lt; 0.001).</div></div><div><h3>Conclusions</h3><div>Violent injury is associated with lower likelihood of receiving rehabilitation services. Subgroup analysis indicates this finding associated with facilities’ selection bias, and this warrants additional study. Efforts should focus on protecting victims of violence from discrimination during the rehabilitation screening process.</div></div>","PeriodicalId":17030,"journal":{"name":"Journal of Surgical Research","volume":"306 ","pages":"Pages 317-326"},"PeriodicalIF":1.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143023859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rare Rib-Originating Solitary Plasmacytoma: Retrospective Analysis and Surgical Outcomes","authors":"Sevinc Citak MD ,&nbsp;Serkan Bayram MD ,&nbsp;Busra Yaprak Bayrak MD ,&nbsp;Mustafa Vayvada MD ,&nbsp;Cagatay Tezel MD","doi":"10.1016/j.jss.2024.12.054","DOIUrl":"10.1016/j.jss.2024.12.054","url":null,"abstract":"<div><h3>Introduction</h3><div>Solitary plasmacytomas are tumors characterized by a local increase of malignant plasma cells in soft tissue or bone and may occur anywhere without evidence of systemic disease. The aim was to focus on the main surgical techniques and outcomes for this rare chest wall tumor.</div></div><div><h3>Methods</h3><div>Patients with solitary plasmacytoma involving a rib, who were operated for diagnostic or treatment purposes between 2018 and 2023 were retrospectively reviewed.</div></div><div><h3>Results</h3><div>Of the six patients included, three were male and the median age was 55.6 (range: 32-74) ys. All patients had preoperative positron emission tomography-computed tomography. Two underwent Tru-cut biopsy before surgery, but only one was then diagnosed. Two patients underwent surgery for diagnosis and the remainder underwent resection and reconstruction. In one patient with both rib and sternum involvement, rib and partial sternum resection was performed and the defect was repaired with mesh. During the 24.1 mos average follow-up period, two patients died, both of whom had metastasis preoperatively. The median survival of all patients was 24.1 (range: 3-63) mos.</div></div><div><h3>Conclusions</h3><div>Solitary plasmacytoma is rarely seen among primary malign tumors of the chest wall. A multidisciplinary approach is important in these cases. Surgical treatment in solitary plasmacytoma has fewer local and systemic side effects compared to radiotherapy and chemotherapy. Therefore, the place of surgery in the treatment of solitary plasmacytoma should be revised. Our findings show that surgery should be considered as not only a palliative treatment, but also a curative one, in solitary plasmacytomas arising in the rib.</div></div>","PeriodicalId":17030,"journal":{"name":"Journal of Surgical Research","volume":"306 ","pages":"Pages 417-423"},"PeriodicalIF":1.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143033321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response Regarding: Adenosine 2A Receptor Agonism Improves Survival in Extracorporeal Cardiopulmonary Resuscitation","authors":"Alex M. Wisniewski MD,&nbsp;Mark E. Roeser MD","doi":"10.1016/j.jss.2024.10.056","DOIUrl":"10.1016/j.jss.2024.10.056","url":null,"abstract":"","PeriodicalId":17030,"journal":{"name":"Journal of Surgical Research","volume":"306 ","pages":"Pages 607-608"},"PeriodicalIF":1.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142895518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Letter Regarding: Perioperative Music Therapy in Colorectal Surgery: A Review of Cost-Effectiveness and Clinical Impact","authors":"Rohan Kapoor MBBS, MS, DNB, MRCS Ed (UK),&nbsp;M. D Ray MS, FRCS (UK), FACS, PhD","doi":"10.1016/j.jss.2024.12.051","DOIUrl":"10.1016/j.jss.2024.12.051","url":null,"abstract":"","PeriodicalId":17030,"journal":{"name":"Journal of Surgical Research","volume":"306 ","pages":"Pages 609-610"},"PeriodicalIF":1.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143028904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Forecasting Pediatric Trauma Volumes: Insights From a Retrospective Study Using Machine Learning","authors":"Ayaka Tsutsumi MD ,&nbsp;Chiara Camerota PhD ,&nbsp;Flavio Esposito PhD ,&nbsp;Si-Min Park MD ,&nbsp;Tiffany Taylor MSN, MBA, RN ,&nbsp;Shin Miyata MD","doi":"10.1016/j.jss.2024.12.010","DOIUrl":"10.1016/j.jss.2024.12.010","url":null,"abstract":"<div><h3>Introduction</h3><div>Rising pediatric firearm-related fatalities in the United States strain Trauma Centers. Predicting trauma volume could improve resource management and preparedness, particularly if daily forecasts are achievable. The aim of the study is to evaluate various machine learning models’ accuracy on monthly, weekly, and daily data.</div></div><div><h3>Methods</h3><div>The retrospective study utilized trauma data between June 1, 2013, and October 31, 2023, from a level I/II pediatric trauma center. Data were organized monthly, weekly, and daily, which further delineated into seven groups, yielding 21 cohorts. Models were evaluated using time-series forecasting metrics. In addition, the models were tested for real-world applicability by forecasting trauma volumes 3 mo, 12 wk, and 31 d ahead for monthly, weekly, and daily predictions respectively. The predicted values were then compared with the actual data.</div></div><div><h3>Results</h3><div>The total of 12,144 patients’ data was utilized to create and evaluate models. 14 forecasting models for each of 21 groups were developed. Monthly predictions generally outperformed weekly and daily ones. Although the Silverkite model excelled in monthly predictions, the one-dimensional convolutional layer model was most accurate for daily predictions. Real-life simulations showed the Prophet model performing best for monthly predictions, with no clear winner for weekly predictions.</div></div><div><h3>Conclusions</h3><div>This study found monthly forecasting most accurate. Although many models outperformed their Naïve counterparts, performance varied by grouping. Real-world simulations confirmed these findings. Despite high accuracy in monthly predictions, the study's generalizability is limited, and daily trauma prediction needs improvement.</div></div>","PeriodicalId":17030,"journal":{"name":"Journal of Surgical Research","volume":"306 ","pages":"Pages 33-42"},"PeriodicalIF":1.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142915087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Using Machine Learning to Predict Weight Gain in Adults: an Observational Analysis From the All of Us Research Program","authors":"Dawda Jawara MD ,&nbsp;Kate V. Lauer MD ,&nbsp;Manasa Venkatesh MS ,&nbsp;Lily N. Stalter MS ,&nbsp;Bret Hanlon PhD ,&nbsp;Matthew M. Churpek MD, MPH, PhD ,&nbsp;Luke M. Funk MD, MPH","doi":"10.1016/j.jss.2024.11.042","DOIUrl":"10.1016/j.jss.2024.11.042","url":null,"abstract":"<div><h3>Introduction</h3><div>Obesity, defined as a body mass index ≥30 kg/m², is a major public health concern in the United States. Preventative approaches are essential, but they are limited by an inability to accurately predict individuals at highest risk of weight gain. Our objective was to develop accurate weight gain prediction models using the National Institutes of Health All of Us dataset. We hypothesized that machine learning models using both electronic health record and behavioral survey data would outperform models using electronic health record data alone.</div></div><div><h3>Methods</h3><div>The All of Us dataset was used to identify adults between 18 and 70 ys old with weight measurements 2 y apart between 2008 and 2022. Patients with a history of cancer, bariatric surgery, or pregnancy were excluded. Demographics, vital signs, laboratory results, comorbidities, and survey data (Alcohol Use Disorder Identification Test, Patient-Reported Outcomes Measurement Information System physical and mental health scores) were included as model parameters. Elastic net and XGBoost machine learning models were developed with and without survey data to predict ≥10% total body weight gain within 2 y. The data were split into a training sample (60%) and a testing sample (40%), and parameters were tuned using 10-fold cross-validation. Performance was compared using area under the receiver operating characteristic curves (AUCs).</div></div><div><h3>Results</h3><div>Our cohort consisted of 34,715 patients (mean [SD] age 50.9 [13.4] y; 45.7% White; 55.3% female). Over a 2-y span, 10.4% of the cohort gained ≥10% total body weight. AUCs were 0.677 [95% DeLong confidence interval 0.665-0.688] for elastic net and 0.706 [0.695-0.717] for XGBoost. Incorporation of survey data did not improve predictability, with AUCs of 0.681 [0.669-0.692] and 0.705 [0.694-0.716], respectively.</div></div><div><h3>Conclusions</h3><div>Our machine learning weight gain prediction models had modest performance that was not improved by survey data. The addition of other All of Us variables, including genomic data, may be informative in future studies.</div></div>","PeriodicalId":17030,"journal":{"name":"Journal of Surgical Research","volume":"306 ","pages":"Pages 43-53"},"PeriodicalIF":1.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142915214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Finding Value in Pediatric Liver Transplantation: When Does Volume Matter?","authors":"Benjamin K. Wang MD ,&nbsp;Madhukar S. Patel MD, MBA, ScM ,&nbsp;Christine Hwang MD ,&nbsp;Cyrus A. Feizpour MD ,&nbsp;Parsia A. Vagefi MD ,&nbsp;Dev M. Desai MD, PhD","doi":"10.1016/j.jss.2024.12.039","DOIUrl":"10.1016/j.jss.2024.12.039","url":null,"abstract":"<div><h3>Introduction</h3><div>Pediatric liver transplantation provides substantial survival benefit. An emphasis on value-based practices has become a central theme in many surgical fields, but have not been well-studied in pediatric transplantation. Given an increasing focus on optimizing outcomes while containing costs, defining value in pediatric liver transplantation warrants investigation.</div></div><div><h3>Methods</h3><div>Pediatric end-stage liver disease -era deceased donor pediatric liver transplant recipients from 2/2002 to 2/2019 were identified using the United Network for Organ Sharing Standard Transplant Analysis <em>file</em> data (<em>n</em> = 5770). Liver centers were divided into volume tertiles (small, medium, and large), and recipients were stratified by age (0-4, 5-11, and 12-18 y). The value for the index transplant episode was defined as % graft survival ≥1 y divided by mean post-transplant length of stay. Nearest-neighbor Mahalanobis metric matching was used to account for confounding when assessing the impact of center volume on value.</div></div><div><h3>Results</h3><div>Compared to small centers, large centers delivered better outcomes (1-y graft survival 93.7% <em>versus</em> 89.4%, <em>P</em> = 0.017) without increased resource utilization (length of stay 20.8 ± 15.6 d <em>versus</em> 19.6 ± 17.0, <em>P</em> = 0.281) during the 17-y study period. Mahalanobois-matched cohorts demonstrated a volume-value relationship (higher value care with better outcomes and decreased resource utilization) in the 0-4 age group, but not in older recipients. The 0-4 age group comprised the largest proportion of status 1B patients (21.8%, <em>P</em> &lt; 0.001) and the highest utilization rate of partial liver allografts (40.9%, <em>P</em> &lt; 0.001).</div></div><div><h3>Conclusions</h3><div>There is value in liver transplant volume in very young (0-4 y) deceased donor pediatric patients. Given improved survival of these patients in higher volume centers, regionalization of care may benefit this specific population of recipients.</div></div>","PeriodicalId":17030,"journal":{"name":"Journal of Surgical Research","volume":"306 ","pages":"Pages 389-396"},"PeriodicalIF":1.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143033293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Comparative Study Between Cold Static Storage and Normothermic Ex-Situ Donor Heart Preservation in a Rat Model of Heterotopic Heart Transplantation","authors":"Mukhammad Kayumov MD, PhD ,&nbsp;Kyo Seon Lee MD, PhD ,&nbsp;Dowan Kim MD, PhD ,&nbsp;Wangin Kim PhD ,&nbsp;Reverien Habimana MD ,&nbsp;Jiae Seong BS ,&nbsp;Hwa Jin Cho MD, PhD ,&nbsp;In-Seok Jeong MD, PhD","doi":"10.1016/j.jss.2024.12.050","DOIUrl":"10.1016/j.jss.2024.12.050","url":null,"abstract":"<div><h3>Introduction</h3><div>Cold static storage (CSS) and normothermic ex-situ preservation are the most widely used donor heart preservation techniques worldwide. The current study compares both CSS and normothermic ex-situ preservation methods in terms of graft performance, morphologic changes, and acute immune response in an experimental model.</div></div><div><h3>Method and materials</h3><div>Twenty rats underwent heterotopic abdominal heart transplantation after 2 h of CSS (group 1; <em>n</em> = 10) or normothermic ex-situ perfusion (group 2; <em>n</em> = 10). Blood samples were obtained from recipients just before and after 4 h of transplantation to analyze surface markers of immune cells and cytokines. Electrocardiography and echocardiography were performed before donor heart harvesting and after heterotopic transplantation. After 4 h of transplantation, donor hearts were extracted for further histologic studies.</div></div><div><h3>Results</h3><div>All recipient animals in both groups successfully survived after heterotopic transplantation. The mean ischemic time of the donor heart was 163 ± 8.34 mins in group 1 and 43.8 ± 6.97 mins in group 2 (<em>P</em> &lt; 0.01). Ejection fraction significantly decreased after transplantation in both groups but were less significant in group 2 (the mean difference group 1: −34.3 ± 3.54, <em>P</em> &lt; 0.01; group 2: −14.3 ± 15.47, <em>P</em> = 0.01). The percentage of granulocyte significantly increased in both group 1 and group 2, but the significance was more pronounced in group 1 (the mean difference group 1: 48.7 ± 5.36, <em>P</em> &lt; 0.01; group 2: 39.7 ± 13.1, <em>P</em> &lt; 0.01).</div></div><div><h3>Conclusions</h3><div>Normothermic ex-situ perfusion is associated with well-preserved donor hearts but a similar recipient acute immune response in comparison with CSS in the rat model.</div></div>","PeriodicalId":17030,"journal":{"name":"Journal of Surgical Research","volume":"306 ","pages":"Pages 437-448"},"PeriodicalIF":1.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143039496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}