Journal of Scientific Research in Science最新文献_第5页

A one-pot Synthesis of Some New Heterocyclic Compounds Derived from Chalcones and Study of their Antitumor and Antimicrobial Activities 查尔酮类杂环化合物的一锅合成及其抗肿瘤和抗菌活性研究

Journal of Scientific Research in Science Pub Date : 2022-12-01 DOI: 10.21608/jsrs.2022.275779

Hadeer M. Hassan, Eman A. Mohamed, B. Awad, Eman M. Mohamed

{"title":"A one-pot Synthesis of Some New Heterocyclic Compounds Derived from Chalcones and Study of their Antitumor and Antimicrobial Activities","authors":"Hadeer M. Hassan, Eman A. Mohamed, B. Awad, Eman M. Mohamed","doi":"10.21608/jsrs.2022.275779","DOIUrl":"https://doi.org/10.21608/jsrs.2022.275779","url":null,"abstract":"The aim of the present work is to efficiently synthesize promising novel antitumor and antimicrobial active heterocyclic compounds from chalcones 1a and 1b as a precursor which contain naphthalene moiety and indole or piperonal moiety, respectively, using conventional, ultrasonic and microwave irradiation techniques. The best yields and purity were afforded with the microwave irradiation technique. Reaction of 1a and 1b with the appropriate reagent gave the corresponding pyrazolines 2a, 2b, pyrimidine-2-thioneses 3a, 3b, oxazepines 4a, 4b, diazepines 5a, 5b, triazolo-pyrimidines 6a, 6b, and pyrimidine-2-thiols 7a, 7b derivatives. Compounds 7a, 7b were used to produce 8a, 8b. Moreover, pyrimidine-2-thione 3a was used to synthesize pyrimidin2-ylthioacetic acid 9a, and 2-hydrazinylpyrido[2,3-d]pyrimidine derivative 10a which has been used as a functionalizing agent to produce compounds 11a-14a. The structural formulas of the synthesized compounds were confirmed by their spectral data; FT-IR, H NMR, C NMR and MS. Compounds 3a, 5a, 7a, 13a showed a very high activity as antitumor, whereas compounds 4a, 6a, and 13a showed high activity as antibacterial and antifungal agents.","PeriodicalId":16981,"journal":{"name":"Journal of Scientific Research in Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85009739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impacts of Genetically Modified Food on Insulin Hormone, Glucose, and Lipid Profile of Male and Female Wister Albino Rats 转基因食品对雄性和雌性白化大鼠胰岛素、激素、葡萄糖和脂质谱的影响

Journal of Scientific Research in Science Pub Date : 2022-12-01 DOI: 10.21608/jsrs.2022.275795

Manal A. Salama, Ola S. Serag El Din, Nehal A. Abd El Wahed

{"title":"Impacts of Genetically Modified Food on Insulin Hormone, Glucose, and Lipid Profile of Male and Female Wister Albino Rats","authors":"Manal A. Salama, Ola S. Serag El Din, Nehal A. Abd El Wahed","doi":"10.21608/jsrs.2022.275795","DOIUrl":"https://doi.org/10.21608/jsrs.2022.275795","url":null,"abstract":"In recent years, genetically modified (GM) crops aim to produce food and feed which has become part of the usual agriculture in many areas of the world. Whether GM food and feed have positive or negative impacts on humans or animals is still unclear. Therefore, an investigation of the effects of different diets containing genetically modified food (GMF) on insulin hormone, glucose, and lipid profile were carried out in the present study. Male and female Wister Albino rats70-80 g range body weight was used in the present study. Each sex was divided into 4 groups (n = 10 per group). Control group fed on the basal diet American Institute of Nutrition for Growth (AIN93 G) and three treated groups were given GM (corn, wheat, and rice) and water adlibitum for three months. The reason for choosing these foods is because they are the most basic foods consumed in our daily life (bread and rice). At the end of the experimental period results compared to the control group GM (corn, wheat, and rice) for both sexes showed a significant decrease in serum insulin and the significant increase in serum glucose. Also, significant increase in total cholesterol (TC) , triglycerides (TG), low density lipoprotein-cholesterol (LDL-C), very low density lipoproteins (VLDL), Risk ratio I and Risk ratio II. Yet, serum high density lipoproteins cholesterol (HDL-C) levels showed significant decrease in all groups. Body weight showed a significant increase in all groups of males and female rats.","PeriodicalId":16981,"journal":{"name":"Journal of Scientific Research in Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76366273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell progression of biofilm formation in Candida albicans and estimation of aspartic proteinase activity 白色念珠菌生物膜形成的细胞进程和天冬氨酸蛋白酶活性的估计

Journal of Scientific Research in Science Pub Date : 2022-12-01 DOI: 10.21608/jsrs.2022.275792

Rowida Elshazly, Shaimaa Selmi Sobieh, Sanaa Sobhy Zaki, Sahar Abdel Fattah Abdel Tawab

{"title":"Cell progression of biofilm formation in Candida albicans and estimation of aspartic proteinase activity","authors":"Rowida Elshazly, Shaimaa Selmi Sobieh, Sanaa Sobhy Zaki, Sahar Abdel Fattah Abdel Tawab","doi":"10.21608/jsrs.2022.275792","DOIUrl":"https://doi.org/10.21608/jsrs.2022.275792","url":null,"abstract":"Yeast cells are commonly used as powerful model system to study morphological phenotype and their processes. The study of morphogenesis of different modes of growth in yeast during biofilm development is an important issue. Cell progression is important tool to understand morphological transition and virulence in C. albicans. Biofilm formation and aspartic proteinase activity are two major important virulence factors associated with the pathogenicity of C. albicans. This study aimed to highlight the formation of biofilm, monitor the development of biofilm progression and estimate the activity of aspartic proteinase. Biofilm formation was assessed by Eliza plate and monitored at different pH values, different incubation time and different media. Biofilm development was visualized microscopically. Cell division and mitotic phases in yeast cells were studied and photographed. The production of aspartic proteinase was assessed by bovine serum albumin agar plates and evaluated at different pH values and different incubation time intervals. Results revealed that C. albicans was positive for biofilm formation and aspartic proteinase production. pH and different media had marked significant effect on biofilm formation. Additionally, the transition from simple yeast budding form to a complex filament structure was time dependent. Moreover, pH showed significant effect on aspartic proteinase activity.","PeriodicalId":16981,"journal":{"name":"Journal of Scientific Research in Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86554798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Identification of Ladybird Beetles (Coccinella: Coccinellidae) Using DNA Barcodes 基于DNA条形码的瓢虫分子鉴定(瓢虫科

Journal of Scientific Research in Science Pub Date : 2022-12-01 DOI: 10.21608/jsrs.2022.275796

Hanan Abdalla, Yosur Gamal Fiteha, Mohamed Abdel-Salam Rashed, Ramadan Mohamed Ali, Aya Mohamed Abdel-Salam Hammad, Ahmed Saber Mohamed Bream, Mahmoud Magdy

{"title":"Molecular Identification of Ladybird Beetles (Coccinella: Coccinellidae) Using DNA Barcodes","authors":"Hanan Abdalla, Yosur Gamal Fiteha, Mohamed Abdel-Salam Rashed, Ramadan Mohamed Ali, Aya Mohamed Abdel-Salam Hammad, Ahmed Saber Mohamed Bream, Mahmoud Magdy","doi":"10.21608/jsrs.2022.275796","DOIUrl":"https://doi.org/10.21608/jsrs.2022.275796","url":null,"abstract":"DNA barcoding is a molecular genetics technique commonly applied for species identification based on the cytochrome c oxidase subunit I (COI) gene located on the mitochondrial DNA genome. Based on our analysis, the DNA barcodes were generated from 16 specimen of Coccinella (family Coccinellidae). All the samples were collected from diverse sites in Egypt and Libya. Morphological traits based on the number of dorsal spots, DNA extraction, PCR amplification of COI, sequencing, nucleotide BLAST, and phylogenetics analyses were used to classify the specimens. A phylogenetic tree was constructed using the maximum likelihood method, including 46 COI sequences (the study and NCBI sequences). Three Coccinella clusters were defined and classified as Coccinella septempunctata, Coccinella novemnotata, and Coccinella undecimpunctata each species clustered into a unique branch. DNA barcodes discriminated clearly against the analyzed species. This study validated the COI efficiency as a marker for DNA barcoding of insects (Coccinella, family Coccinellidae).","PeriodicalId":16981,"journal":{"name":"Journal of Scientific Research in Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75080711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Trends in the assessment of multidrug efficiency against identified bacterial strains isolated from wounds at El-Demerdash Hospital, Egypt 对埃及El-Demerdash医院伤口分离出的已确定菌株的多药疗效评估趋势

Journal of Scientific Research in Science Pub Date : 2022-12-01 DOI: 10.21608/jsrs.2022.275789

Howida R. Mohammed, Zeinab M.H. Kheiralla, Maha A. Hewedy, Ayman F. Ahmed, Elham E. Moustafa, Salah Abdelbary

{"title":"Trends in the assessment of multidrug efficiency against identified bacterial strains isolated from wounds at El-Demerdash Hospital, Egypt","authors":"Howida R. Mohammed, Zeinab M.H. Kheiralla, Maha A. Hewedy, Ayman F. Ahmed, Elham E. Moustafa, Salah Abdelbary","doi":"10.21608/jsrs.2022.275789","DOIUrl":"https://doi.org/10.21608/jsrs.2022.275789","url":null,"abstract":"Multidrug-resistant (MDR) bacteria is a severe problem for universal public health which increases morbidity and mortality rate. These resistant bacteria lead to ineffective treatment of drugs resulting in the spreading and persistence of infections. So, the major target of this study is to estimate the competence of multidrug antibacterial agents against bacterial strains isolated from wound samples and then identify the most potent Multidrug-resistant (MDR) bacteria. Fifty wound swab specimens were gathered from various wounds and several patients from the Central Microbiology Laboratory of El-Demerdash Hospital, Cairo, Egypt. Eightynine bacterial isolates were isolated from fifty wound samples then cultured on different media and tested for their susceptibility to different thirty antibiotic discs using the agar disc diffusion method. After recording the results of the susceptibility test, the post potent resistant bacterial isolates recorded 3 bacterial isolates which resistant to 30 different antibiotic types. These resistant bacterial isolates were identified using morphological, biochemical, and molecular techniques. The results recorded that the post potent resistant bacterial isolates identified as Klebsiella oxytoca, Pseudomonas aeruginosa, and Escherichia coli. This study concluded that with the increase in the random use of antibiotic drugs resulted in the presence of multi-antibacterial resistant strains. There are bacterial strains that were isolated from wounds in patients at El-Demerdash Hospital, Egypt, and identified. They can resist about thirty different antibiotic discs. Abbreviation: Multiple antibiotic resistance (MAR).","PeriodicalId":16981,"journal":{"name":"Journal of Scientific Research in Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78823596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibiotic resistant and mecA gene characterization of Staphylococcus epidermidis isolated from five hospitals in Gaza strip 从加沙地带五家医院分离的表皮葡萄球菌的抗生素耐药性和mecA基因特征

Journal of Scientific Research in Science Pub Date : 2022-02-28 DOI: 10.21608/jsrs.2020.41139.1026

M. Alnajar, S. Abdelsalam, N. E. El Aila, B. Ayesh, M. Fahd

引用次数: 0

Reproductive Toxicity Induced by Low Dose Bisphenol A (BPA) in Male Rats 低剂量双酚A (BPA)对雄性大鼠生殖毒性的影响

Journal of Scientific Research in Science Pub Date : 2022-02-28 DOI: 10.21608/jsrs.2020.40944.1029

sara elshafiey, M. El-Gendy, Mohamed Rashed, Ramadan Mohamed Ali, A. Kamel

引用次数: 0

Efficiency of transgenic Chlamydomonas reinhardtii for removing of toxicity of cyanide compound 转基因莱茵衣藻对氰化物的脱毒效果

Journal of Scientific Research in Science Pub Date : 2021-12-26 DOI: 10.21608/jsrs.2021.85161.1061

Rasha Elgammal, Y. El-Ayouty, S. Sobieh, wafaa abouelkheir, A. EL-Sheimy, A. Soror

{"title":"Efficiency of transgenic Chlamydomonas reinhardtii for removing of toxicity of cyanide compound","authors":"Rasha Elgammal, Y. El-Ayouty, S. Sobieh, wafaa abouelkheir, A. EL-Sheimy, A. Soror","doi":"10.21608/jsrs.2021.85161.1061","DOIUrl":"https://doi.org/10.21608/jsrs.2021.85161.1061","url":null,"abstract":"In this study, we used the Agrobacterium tumefaciens–mediated transformation approach to transfer the activity of the cyanobacterial cyanase enzyme into the microalga, Chlamydomonas reinhardtii. In transgenic C. reinhardtii strains, the recombinant cyanase enzyme was proven to be active. In comparison to wild-type control, transgenic type demonstrated higher rates of ammonia release, reduced loss of pigmentation, and a lower percentage of growth retardation when varied amounts of cyanide (up to 200 ppm) were applied to the growth medium. Moreover, cyanase activity increases as concentration of cyanide increase especially in case of transgenic. The maximum activity was indicated in presence of 100 mg/l cyanide it reached eight folds more than wild activity at the same cyanide concentration. Results of this study provide an effective eco-friendly phytoremediation system for detoxification of cyanide using micro-alga compared to previously reported conventional system for removal of cyanide compounds, Also, some factors are taken in consideration like different pH, contact time and the transgenic type has been the priority for removal cyanide at wide range of pH with two folds more than wild type.","PeriodicalId":16981,"journal":{"name":"Journal of Scientific Research in Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83692626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Effect of Pb+2 ion on physico chemical properties of fly ash -slag geopolymer pastes Pb+2离子对粉煤灰-矿渣地聚合物膏体理化性能的影响

Journal of Scientific Research in Science Pub Date : 2021-12-26 DOI: 10.21608/jsrs.2021.62415.1051

S. Abdallah, doaa abdelmonium, Sheren M. Ragai

引用次数: 2

Morphological and Quantitative traits of phylogenetic relationships of some barley (Hordeum vulgare L.) accessions in Egypt 埃及部分大麦(Hordeum vulgare L.)种质的形态和数量性状的系统发育关系

Journal of Scientific Research in Science Pub Date : 2021-12-26 DOI: 10.21608/jsrs.2021.54324.1041

sarah shata, Wafaa Morsy, fatthy abdel-tawab, Lamyaa Kamal

引用次数: 0