Journal of Pure and Applied Microbiology最新文献_第7页

Evaluating the Antibacterial Profile of Copper Oxide Nanoparticles – Vitamin E (CuO NPs-Vit E) Complex against Multidrug-resistant Escherichia coli and Staphylococcus aureus 氧化铜纳米颗粒-维生素E (CuO NPs-Vit E)复合物对多重耐药大肠杆菌和金黄色葡萄球菌的抑菌性能评价

Journal of Pure and Applied Microbiology Pub Date : 2023-10-02 DOI: 10.22207/jpam.17.4.01

Haris Variyathody, Malarvizhi Arthanari, Manohar Murugan, Gobianand Kuppannan

{"title":"Evaluating the Antibacterial Profile of Copper Oxide Nanoparticles – Vitamin E (CuO NPs-Vit E) Complex against Multidrug-resistant Escherichia coli and Staphylococcus aureus","authors":"Haris Variyathody, Malarvizhi Arthanari, Manohar Murugan, Gobianand Kuppannan","doi":"10.22207/jpam.17.4.01","DOIUrl":"https://doi.org/10.22207/jpam.17.4.01","url":null,"abstract":"Millions of people die every year as a result of infections’ multidrug resistance (MDR) pathogens. Therefore, the creation of novel antibiotics is urgently required for the treatment of bacterial illnesses that are resistant to a variety of drugs. The current study concentrated on the development of copper oxide nanoparticles conjugated with Vitamin-E (CuO NPs-Vit E complex), which was evaluated using various approaches like FTIR (Fourier transform infrared spectroscopy), UV-Vis (ultraviolet-visible spectroscopy), SEM (scanning electron microscopy), XRD (X-ray diffraction). The antibacterial and anti-biofilm activity was investigated against MDR Escherichia coli and Staphylococcus aureus. The antioxidant properties of CuO NPs-Vit E complex were determined using DPPH and FRAP assay. The CuO NPs-Vit E complex showed excellent anti-bacterial, anti-biofilm, and antioxidant activities. Moreover, it was found to be biocompatible and non-toxic to normal cells. Therefore, the synthesized CuO NPs-Vit E complex can be used for the creation of new drugs for the treatment of multidrug-resistant bacterial infections.","PeriodicalId":16968,"journal":{"name":"Journal of Pure and Applied Microbiology","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135830049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacterial Etiologies and Antibiotic Sensitivities in Acute and Chronic Dacryocystitis: A Western U.P. Perspective 急性和慢性泪囊炎的细菌病原学和抗生素敏感性：西方大学的观点

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Journal of Pure and Applied Microbiology Pub Date : 2023-09-03 DOI: 10.22207/jpam.17.3.52

S. Rizvi, N. Mehrotra, Amrita Bajpai Mishra, Neelam Gupta, Sakshi Singh, Ria Batra

{"title":"Bacterial Etiologies and Antibiotic Sensitivities in Acute and Chronic Dacryocystitis: A Western U.P. Perspective","authors":"S. Rizvi, N. Mehrotra, Amrita Bajpai Mishra, Neelam Gupta, Sakshi Singh, Ria Batra","doi":"10.22207/jpam.17.3.52","DOIUrl":"https://doi.org/10.22207/jpam.17.3.52","url":null,"abstract":"The current study aimed to assess and compare the bacteriological spectrum of acute and chronic dacryocystitis and the antibiotic susceptibility and resistance of the causative pathogens to commonly used antimicrobials. This was a prospective observational study. Cases of dacryocystitis were categorized as acute or chronic, based on clinical features. Specimens were obtained by sterile cotton swabs from the lower conjunctival fornix and from the puncta by applying pressure over the lacrimal sac area or by performing lacrimal syringing. Specimens were inoculated on appropriate culture media and incubated at 37ºC for 24-48 hours. Bacterial species were identified based on colony morphology and standard biochemical tests. Antibiotic Susceptibility Testing was assessed by Kirby Bauer disc diffusion technique using Mueller Hinton agar following Clinical and Laboratory Standards Institute guidelines. Out of 50 patients, 37 (74%) had chronic dacryocystitis and 13 (26%) had acute dacryocystitis. 35 bacterial species were recovered. Gram-positive organisms were the most isolated organisms i.e., 27 out of 35 (77.2%). In chronic dacryocystitis, the predominant bacterial species were Staphylococcus epidermidis (36%). In acute dacryocystitis, the predominant bacterial species were Staphylococcus aureus (40%). Against gram-positive organisms, Vancomycin and Linezolid were most effective (100%). Against gram-negative bacterial species, Amikacin was most effective (100%). High prevalence rate of antibiotic resistance was found, with 40% of the total bacterial species resistant to 5 or more antibiotics. The alarming rate of multi-drug resistance underscores the imperative need for tailored antibiotic strategies and continuous monitoring. Evidence based antibiotic therapy may also help to prevent failures of DCR, progression to chronicity and antibiotic resistance.","PeriodicalId":16968,"journal":{"name":"Journal of Pure and Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45111532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial effect of Tetraspanin CD9 Peptides on Pseudomonas aeruginosa Tetraspanin CD9肽对铜绿假单胞菌的抗菌作用

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Journal of Pure and Applied Microbiology Pub Date : 2023-09-03 DOI: 10.22207/jpam.17.3.41

Khairiyah Murad, S. Ab-Rahim, H. Al-Talib

{"title":"Antimicrobial effect of Tetraspanin CD9 Peptides on Pseudomonas aeruginosa","authors":"Khairiyah Murad, S. Ab-Rahim, H. Al-Talib","doi":"10.22207/jpam.17.3.41","DOIUrl":"https://doi.org/10.22207/jpam.17.3.41","url":null,"abstract":"It is critical to find an alternative therapeutic approach to combat Pseudomonas aeruginosa (P. aeruginosa) that can simultaneously reduce the occurrence of bacterial resistance. The tetraspanin CD9, a highly expressed membrane protein in melanocytes was chosen for this study because it is highly expressed in keratinocytes and has been implicated in the pathogenesis of bacterial infections in a previous study. The antimicrobial activity of CD9 peptides against the standard strain P. aeruginosa (ATCC 27853) and a clinical multidrug-resistant P. aeruginosa (MDR- P. aeruginosa) was studied using the disc diffusion method. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of CD9 peptides were determined by broth microdilution assays with concentrations ranging from 1 mg/mL to 4.88×10-4 mg/mL. The antibiofilm activity of the CD9 peptides was also determined. CD9 peptides showed an 11.75 ± 2.36 mm inhibition zone against the standard P. aeruginosa strain but none against the MDR- P. aeruginosa. Both isolates had the same MIC value, 0.25 mg/mL. The MBC for the standard strain P. aeruginosa was 0.5 mg/mL, while for the MDR- P. aeruginosa strain, it was 1 mg/mL. CD9 peptides significantly inhibited up to 70% biofilm against both P. aeruginosa isolates. CD9 peptides showed a modest inhibitory effect against the standard strain P. aeruginosa but not against MDR- P. aeruginosa. Interestingly, CD9 peptides were found to be a good anti-biofilm treatment against both P. aeruginosa isolates. This study demonstrated that CD9 peptides have the potential to be an alternative antimicrobial treatment against P. aeruginosa.","PeriodicalId":16968,"journal":{"name":"Journal of Pure and Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48076699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Polish Adults’ Knowledge, Perceptions and Attitudes Concerning Legionellosis 波兰成年人对军团病的认识、认知和态度

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Journal of Pure and Applied Microbiology Pub Date : 2023-09-03 DOI: 10.22207/jpam.17.3.47

N. Shalan, Zuzanna Laszuk, I. Kosińska, Krzysztof Kanecki, Mohd. Alaraj

{"title":"Polish Adults’ Knowledge, Perceptions and Attitudes Concerning Legionellosis","authors":"N. Shalan, Zuzanna Laszuk, I. Kosińska, Krzysztof Kanecki, Mohd. Alaraj","doi":"10.22207/jpam.17.3.47","DOIUrl":"https://doi.org/10.22207/jpam.17.3.47","url":null,"abstract":"Despite the growing prevalence of legionellosis in Poland and worldwide, little is known about the extent of public awareness regarding the seriousness of this disease and the appropriate preventive measures. The aim of this work is to assess the Polish adults’ knowledge, perceptions, and beliefs about legionellosis and its causative agents, risk factors, exposure, and other relevant facts. Data for this cross-sectional study were gathered via a questionnaire that was constructed and validated by the study investigators before commencing the survey, which lasted from January to March 2022. Knowledge, attitude and practice towards legionella were measured and quantified. One-way ANOVA and chi square tests were used to compare between demographic variables and the level of knowledge. Regression analysis was conducted to examine the predictors for higher knowledge among study participants. A total of 251 participants with a mean age of 28.26 ± 9.6 were enrolled in the current study. Over two thirds (74%) were females, with higher education (62%). Older age was associated with less knowledge about legionellosis (B = -0.049, p < 0.001), while higher education was associated with more knowledge (B = 1.656, p < 0.001). No significant differences were found between genders (p = 0.066). A knowledge gap was present for diagnostic tests regarding legionella. On the other hand, knowledge about prevention procedures was quite high among study participants. This study showed that overall knowledge about legionellosis in Polish adults was quite low. In particular, older age groups and the less educated are in need of more awareness of legionellosis disease. A knowledge gap was particularly present regarding how the disease is diagnosed. Awareness campaigns containing simple, easy-to-understand information could prove useful in combating the disease.","PeriodicalId":16968,"journal":{"name":"Journal of Pure and Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42902960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Comprehensive Study of Phenotypic and Genotypic Techniques to Detect Metallo-β-lactamases in Carbapenem-resistant Escherichia coli (E. coli) and Klebsiella pneumoniae (KP) Strains Derived through Numerous Clinical Specimens in Advanced Health care Facilities 先进医疗机构大量临床标本衍生的耐碳青霉烯型大肠杆菌(E. coli)和肺炎克雷伯菌(KP)菌株金属β-内酰胺酶表型和基因型检测技术的综合研究

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Journal of Pure and Applied Microbiology Pub Date : 2023-09-03 DOI: 10.22207/jpam.17.3.54

Manju, Amandeep Kaur, Ashwini Manhas, K. Kaur, Gundeep Kaur, Ram Gopal Saini, Priyanka, Manju Singh

{"title":"A Comprehensive Study of Phenotypic and Genotypic Techniques to Detect Metallo-β-lactamases in Carbapenem-resistant Escherichia coli (E. coli) and Klebsiella pneumoniae (KP) Strains Derived through Numerous Clinical Specimens in Advanced Health care Facilities","authors":"Manju, Amandeep Kaur, Ashwini Manhas, K. Kaur, Gundeep Kaur, Ram Gopal Saini, Priyanka, Manju Singh","doi":"10.22207/jpam.17.3.54","DOIUrl":"https://doi.org/10.22207/jpam.17.3.54","url":null,"abstract":"In recent years, an increase in Escherichia coli and Klebsiella pneumoniae resistant to carbapenem was reported globally. Due to their high prevalence and extensive range of medical conditions, Escherichia coli and Klebsiella pneumoniae are both confirmed to be major public health concerns. Furthermore, carbapenem resistance restricts treatment options for individuals infected with these bacteria. Consequently, early detection of carbapenem resistance is essential for starting effective therapy, achieving successful management, and avoiding the infection from spreading further in the future. This study’s objective was to identify the phenotypic and genotypic identification of Metallo-β-lactamases (MBL) in carbapenem-resistant E. coli and K. pneumoniae in advanced healthcare facilities. Meropenem resistance was tested in E. coli and K. pneumoniae using the Kirby-Bauer disc diffusion technique. MBL was discovered using a combination of Disc diffusion testing and the Modified Hodge Test. The Polymerase Chain Reaction was used to determine the genotypes of the bla NDM-1 genes that express these enzymes. Out of 427 strains, including 223 E. coli and 204 K. pneumoniae, 35 (8.2%) consisted of carbapenem-resistant, and 29 (82.85%) showed phenotypically verified as metallo-beta-lactamase producers by using the Combined disc test and 20 (57.14%) using the Modified Hodge test. Polymerase Chain Reaction tests for genes detect those three different strains all showed the bla NDM-1 gene. Carbapenemase production and MBL can be recognized with the help of phenotypic combination disc and MHT tests in labs. Since both tests showed 100% concordance, laboratories may use the less expensive CDT instead of the MHT. The current study supports institutional antibiotic stewardship programmes to manage antibiotic use and prevent CRE worldwide.","PeriodicalId":16968,"journal":{"name":"Journal of Pure and Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46082355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Efficacy and Safety of Heterologous Immunization with Pfizer-BioNTech (Pfizer) to Individuals Who Have Completed A Primary Vaccination Schedule with Sinopharm (BBibp-CorV) 辉瑞生物技术公司（Pfizer）对完成国药集团（BBibp-CorV）初级疫苗接种计划的个人进行异源免疫的有效性和安全性

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Journal of Pure and Applied Microbiology Pub Date : 2023-09-03 DOI: 10.22207/jpam.17.3.43

N. Hussein, Warveen L. Abdulkareem, N. Rasheed, Mohammed Rashid Ameen

{"title":"The Efficacy and Safety of Heterologous Immunization with Pfizer-BioNTech (Pfizer) to Individuals Who Have Completed A Primary Vaccination Schedule with Sinopharm (BBibp-CorV)","authors":"N. Hussein, Warveen L. Abdulkareem, N. Rasheed, Mohammed Rashid Ameen","doi":"10.22207/jpam.17.3.43","DOIUrl":"https://doi.org/10.22207/jpam.17.3.43","url":null,"abstract":"The aim of this study was to examine the effectiveness and potential adverse reactions when providing a different Pfizer-BioNTech booster shot to 235 volunteers who had previously received the BBIBP-CorV Sinopharm primary vaccination series. Between February and December 2022, a questionnaire-based cross-sectional study was conducted in Duhok, located within the Kurdistan Region of Iraq. The individuals included in the study were adults aged 18 and above, who had received a Pfizer-BioNTech booster shot following the completion of a two-dose vaccination regimen with Sinopharm (BBIBP-CorV). The findings revealed that among those vaccinated with BBIBP-CorV, there were breakthrough infections at a rate of 4.26%, and no significant correlation was identified between post-vaccination infections and factors such as demographics or medical history. Furthermore, individuals who had a Pfizer booster dose experienced breakthrough infections at a rate of 5.73%, and similarly, no link was discovered between this rate and demographic or medical factors. Additionally, the study uncovered that participants commonly experienced side effects, primarily consisting of mild effects at the injection site. The study implies that both the Sinopharm and Pfizer vaccines demonstrate satisfactory safety profiles. It also suggests that giving a heterologous booster dose to individuals who have finished their primary vaccination with the BBIBP-CorV vaccine offers a significant level of protection against infection.","PeriodicalId":16968,"journal":{"name":"Journal of Pure and Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45931212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative and Prospective Study on the Efficacy of RT-PCR and Rapid Antigen Test in Symptomatic COVID-19 Patients at Tertiary Care Hospital RT-PCR与快速抗原检测在三级医院COVID-19症状患者中的疗效对比与前瞻性研究

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Journal of Pure and Applied Microbiology Pub Date : 2023-09-03 DOI: 10.22207/jpam.17.3.49

Ramya Sree Allavarapu, K. Sethumadhavan, Purimitla Usharani, B.V.V.V. Tejaswani

{"title":"Comparative and Prospective Study on the Efficacy of RT-PCR and Rapid Antigen Test in Symptomatic COVID-19 Patients at Tertiary Care Hospital","authors":"Ramya Sree Allavarapu, K. Sethumadhavan, Purimitla Usharani, B.V.V.V. Tejaswani","doi":"10.22207/jpam.17.3.49","DOIUrl":"https://doi.org/10.22207/jpam.17.3.49","url":null,"abstract":"The COVID-19 pandemic has primarily been controlled by testing for SARS-CoV-2 infections. Despite vaccines, testing will remain crucial for surveillance and screening, allowing for the detection of new variants in a timely manner and to isolate the infected people to lower the danger of the disease spreading further. The research study attempts to found out the efficiency of Reverse-transcriptase polymerase chain reaction (RT-PCR) and Rapid antigen tests in symptomatic COVID-19 patients at tertiary care hospitals. The research was performed on 1000 patients, both In-patients and Out-patients, who presented with COVID-19 symptoms. SARS-COV-2 nucleocapsid protein antigen was detected qualitatively with rapid antigen test in human nasal specimens through the immuno-chromatographic assay. The rapid test results were compared with a molecular test RT-PCR in which FAM, HEX, and ROX were the indicator dyes for the RdRp gene, E gene, and the internal control (RNAse P), respectively. Nearly 322 cases were positive with both RT-PCR and rapid antigen test methods. Fifty-nine samples yielded negative results with the rapid antigen test and positive with PCR. Three samples were negative with RT-PCR and positive with the rapid antigen test. The findings from our study show that the common symptoms are fever 92.2% and cough 74.1% in the reported test population. But in confirmed cases of RT-PCR showed cough at 74.1% was more prevalent, followed by fever at 41.3%. Rapid antigen test showed a overall sensitivity and specificity of 85.3% and 99.5%. According to World Health Organization, rapid antigen detection tests meet the minimum performance requirements of ≥80% sensitivity and ≥97% specificity. Hence, the present study meets this criterion and may perhaps be a probable tool for point-of-care in hospital settings.","PeriodicalId":16968,"journal":{"name":"Journal of Pure and Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43891460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Retrospective Study to Determine the Genotypic Distribution of Hepatitis-C from a Tertiary Care Hospital in South India 印度南部三级医院丙型肝炎基因型分布的回顾性研究

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Journal of Pure and Applied Microbiology Pub Date : 2023-09-03 DOI: 10.22207/jpam.17.3.51

B. Appalaraju, M. Rizwana

{"title":"A Retrospective Study to Determine the Genotypic Distribution of Hepatitis-C from a Tertiary Care Hospital in South India","authors":"B. Appalaraju, M. Rizwana","doi":"10.22207/jpam.17.3.51","DOIUrl":"https://doi.org/10.22207/jpam.17.3.51","url":null,"abstract":"Hepatitis C virus (HCV) is a blood-borne pathogen that transmits infection via transfusion. Hepatocellular carcinoma is the fifth most common cancer and a major cause of death in patients with chronic HCV infection. Response to treatment is mainly based on the genotypic characterization of HCV. The gold standard for genotyping HCV is by sequencing highly conserved regions such as NS5, core, E1, and 5’UTR. Serum samples of patients who visited the tertiary care hospital with clinical features suggestive of HCV infection formed the study group. HCV genotyping was performed using multiplex Polymerase Chain Reaction in the samples tested positive by Chemiluminescence Immunoassay (CLIA). The viral loads were also performed on selected patient samples. In the present study, Genotype 4 (35.71%), followed by Genotype 3 (17.53%) and 1 & 1b (12.34%) were the common genotypes observed. Genotype 1,1b & 4 mixed type and genotype 4 and 5 mixed type was detected in one sample each (0.65%). The mean measured value of HCV antibody was 11.51 ± 4.57. The viral load was detected in 61 out of 81 samples tested. The mean viral load ranged from 550 to 552769250IU/ml (log 2.74-log 8.74). Genotype 4 was the most common genotype demonstrated in our study as opposed to the other studies were genotype 3 was the dominant one in south India.","PeriodicalId":16968,"journal":{"name":"Journal of Pure and Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47260422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rhizosphere Microorganisms with Different Strategies and Mechanisms to Enhance Plant Growth in the Occurrence of Different Environmental Stress Factors 不同环境胁迫条件下根际微生物促进植物生长的策略和机制

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Journal of Pure and Applied Microbiology Pub Date : 2023-09-03 DOI: 10.22207/jpam.17.3.59

Mona Othman I. Albureikan

{"title":"Rhizosphere Microorganisms with Different Strategies and Mechanisms to Enhance Plant Growth in the Occurrence of Different Environmental Stress Factors","authors":"Mona Othman I. Albureikan","doi":"10.22207/jpam.17.3.59","DOIUrl":"https://doi.org/10.22207/jpam.17.3.59","url":null,"abstract":"Every minute, the world’s population grows, and in order to feed them, crop output and agricultural productivity must be improved by adding crucial microorganisms that boost plant yields in various ways through nitrogen fixation, the secretion of both plant growth regulators and 1-aminocyclopropane 1-carboxylate deaminase, as well as some antimicrobial agents. Numerous endophytic bacteria have recently been used to increase plant yields, and agricultural production in addition to reducing salt stresses. Many scientists have made an effort to clarify and comprehend the processes by which bacteria promote plant growth and production. A vital substance known as 1-aminocyclopropane-1-carboxylate (ACC) deaminase is produced by several bacteria, plants, and fungi to decrease ethylene levels in a plant grown under different environmental stress. The gaseous hormone ethylene (C2H4) is synthesized in plant tissues from the precursor ACC, and it has numerous biochemical roles in plants, such as cells differentiation and tissue development, seedling, root hair, leaf, and flower growth and development in addition to fruit ripening and formation of anthocyanin and volatile compounds. Thus, this critical enzyme had influential roles in plants during their positive interaction with bacteria which increase plant growth due to auxin production and protect plants against different environmental stress like drought, high salts, wilting, high level of heavy metals, contaminants with pesticides, and microbial pathogen infections. Different bacterial genera are highly ACC deaminase-producer, and these bacteria support plant growth and agricultural process. In conclusion, bacteria can replace chemicals in a variety of environmentally benign methods to boost soil fertility and plant productivity. However, much research is required to determine the efficacy of these bacteria before suggesting their use on a broad scale in the field.","PeriodicalId":16968,"journal":{"name":"Journal of Pure and Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46041010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibacterial Potential of Oyster Mushroom (Pleurotus ostreatus (Jacq. Ex Fr.) P. Kumm.) Extract against Pathogenic Bacteria 平菇的抗菌潜力抗病原菌提取物

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Journal of Pure and Applied Microbiology Pub Date : 2023-09-03 DOI: 10.22207/jpam.17.3.56

W. Sutthisa, Supawadee Anujakkawan

{"title":"Antibacterial Potential of Oyster Mushroom (Pleurotus ostreatus (Jacq. Ex Fr.) P. Kumm.) Extract against Pathogenic Bacteria","authors":"W. Sutthisa, Supawadee Anujakkawan","doi":"10.22207/jpam.17.3.56","DOIUrl":"https://doi.org/10.22207/jpam.17.3.56","url":null,"abstract":"This study investigated the antibacterial properties of Pleurotus ostreatus (Jacq. Ex Fr.) P. Kumm. extracts against various pathogenic bacteria. The fruiting bodies of fresh oyster mushrooms were collected from Mae Phai Boon Mushroom Farm in Ban Thung Nang Rao, Mueang District, Mahasarakham Province, and subjected to extraction using 95% ethanol and 95% ethyl acetate solvents. The effectiveness of the extracts in suppressing the proliferation of pathogenic microorganisms, encompassing Bacillus cereus, Enterobacter cloacae, Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa, Salmonella typhi, Serratia marcescens, and Staphylococcus aureus, was assessed through the utilization of the paper disk diffusion technique. The results revealed that the crude extract obtained from the 95% ethanol solvent exhibited significant inhibitory effects against B. cereus, E. cloacae, P. aeruginosa, S. marcescens, and S. typhi, yielding inhibition zone diameters from 9.22 to 12.33 mm. In contrast, the crude extract from the 95% ethyl acetate solvent showed inhibitory activity only against E. coli, revealing an inhibition zone diameter of 12.00 mm. Additionally, the determination of the minimum inhibitory concentration (MIC) of the 95% ethanol crude extract against P. aeruginosa and S. marcescens established a value of 150 mg/ml, and concomitantly, the minimal bactericidal concentration (MBC) was also established at 150 mg/ml. However, it was observed that the 95% ethanol crude extract at a concentration of 15 mg/ml was incapable of suppressing the proliferation of E. coli in the MIC evaluations. These findings imply that the extracts originating from P. ostreatus possess inherent capacity as organic antibacterial agents targeting specific pathogenic bacteria. Therefore, these findings justify the necessity for additional scrutiny into their conceivable utilities within the domains of medicine and food preservation.","PeriodicalId":16968,"journal":{"name":"Journal of Pure and Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45553015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0