RT-PCR与快速抗原检测在三级医院COVID-19症状患者中的疗效对比与前瞻性研究

IF 0.7 Q4 MICROBIOLOGY
Ramya Sree Allavarapu, K. Sethumadhavan, Purimitla Usharani, B.V.V.V. Tejaswani
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引用次数: 0

摘要

COVID-19大流行主要是通过检测SARS-CoV-2感染来控制的。尽管有疫苗,但检测对于监测和筛查仍然至关重要,可以及时发现新的变异,并隔离受感染者,以降低疾病进一步传播的危险。本研究旨在了解逆转录聚合酶链反应(RT-PCR)和快速抗原检测在三级医院COVID-19症状患者中的有效性。该研究对1000名出现COVID-19症状的住院和门诊患者进行了研究。采用快速抗原试验,采用免疫层析法对人鼻标本中SARS-COV-2核衣壳蛋白抗原进行定性检测。快速检测结果与以FAM、HEX和ROX分别作为RdRp基因、E基因和内控(RNAse P)指示染料的分子检测RT-PCR进行比较。近322例RT-PCR和快速抗原检测均呈阳性。59份标本快速抗原检测结果为阴性,PCR检测结果为阳性。3份标本RT-PCR阴性,快速抗原试验阳性。我们的研究结果显示,在报告的检测人群中,常见的症状是发烧92.2%,咳嗽74.1%。但在确诊病例中,RT-PCR显示咳嗽更为普遍,占74.1%,其次是发烧,占41.3%。快速抗原试验的总敏感性为85.3%,特异性为99.5%。根据世界卫生组织的规定,快速抗原检测技术的最低性能要求是灵敏度≥80%,特异性≥97%。因此,目前的研究符合这一标准,也许是一个可能的工具,在医院设置的护理点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparative and Prospective Study on the Efficacy of RT-PCR and Rapid Antigen Test in Symptomatic COVID-19 Patients at Tertiary Care Hospital
The COVID-19 pandemic has primarily been controlled by testing for SARS-CoV-2 infections. Despite vaccines, testing will remain crucial for surveillance and screening, allowing for the detection of new variants in a timely manner and to isolate the infected people to lower the danger of the disease spreading further. The research study attempts to found out the efficiency of Reverse-transcriptase polymerase chain reaction (RT-PCR) and Rapid antigen tests in symptomatic COVID-19 patients at tertiary care hospitals. The research was performed on 1000 patients, both In-patients and Out-patients, who presented with COVID-19 symptoms. SARS-COV-2 nucleocapsid protein antigen was detected qualitatively with rapid antigen test in human nasal specimens through the immuno-chromatographic assay. The rapid test results were compared with a molecular test RT-PCR in which FAM, HEX, and ROX were the indicator dyes for the RdRp gene, E gene, and the internal control (RNAse P), respectively. Nearly 322 cases were positive with both RT-PCR and rapid antigen test methods. Fifty-nine samples yielded negative results with the rapid antigen test and positive with PCR. Three samples were negative with RT-PCR and positive with the rapid antigen test. The findings from our study show that the common symptoms are fever 92.2% and cough 74.1% in the reported test population. But in confirmed cases of RT-PCR showed cough at 74.1% was more prevalent, followed by fever at 41.3%. Rapid antigen test showed a overall sensitivity and specificity of 85.3% and 99.5%. According to World Health Organization, rapid antigen detection tests meet the minimum performance requirements of ≥80% sensitivity and ≥97% specificity. Hence, the present study meets this criterion and may perhaps be a probable tool for point-of-care in hospital settings.
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来源期刊
Journal of Pure and Applied Microbiology
Journal of Pure and Applied Microbiology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-MICROBIOLOGY
CiteScore
2.00
自引率
0.00%
发文量
266
审稿时长
11 months
期刊介绍: Journal of Pure and Applied Microbiology (JPAM) is a peer-reviewed, open access international journal of microbiology aims to advance and disseminate research among scientists, academics, clinicians and microbiologists around the world. JPAM publishes high-quality research in all aspects of microbiology in both online and print form on quarterly basis.
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