Journal of Pure and Applied Microbiology最新文献_第3页

Antimicrobial Activity of Black Soldier Fly, Hermetia illucens (Diptera: Stratiomyidae) Larval Hemolymph against Various Pathogenic Bacteria 黑兵蝇（Hermetia illucens，双翅目：Stratiomyidae）幼虫血淋巴对各种病原菌的抗菌活性

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Journal of Pure and Applied Microbiology Pub Date : 2023-11-30 DOI: 10.22207/jpam.17.4.47

N. Azmiera, H. Al-Talib, Noraziah Sahlan, A. Krasilnikova, S. Sahudin, C. Heo

{"title":"Antimicrobial Activity of Black Soldier Fly, Hermetia illucens (Diptera: Stratiomyidae) Larval Hemolymph against Various Pathogenic Bacteria","authors":"N. Azmiera, H. Al-Talib, Noraziah Sahlan, A. Krasilnikova, S. Sahudin, C. Heo","doi":"10.22207/jpam.17.4.47","DOIUrl":"https://doi.org/10.22207/jpam.17.4.47","url":null,"abstract":"The larvae of the black soldier fly (BSFL), Hermetia illucens (Linnaeus, 1758) (Diptera: Stratiomyidae), can survive in environments contaminated with various bacteria by producing antimicrobial compounds. This study, for the very first time, investigated the potential antibacterial activity of hemolymph extracted from BSFL in Malaysia using diffusion and dilution methods. Prior to extraction, the larvae were infected with either Methicillin-resistant Staphylococcus aureus (MRSA) or Escherichia coli. Then, the hemolymph was collected. Serial dilutions from 200 to 12.5 mg/ml of the hemolymph extracts were screened against ten different bacteria. The results showed inhibition of eight out of ten tested bacteria (i.e., MRSA, Staphylococcus aureus, Streptococcus pyogenes, Bacillus subtilis, Micrococcus luteus, Escherichia coli, Klebsiella pneumoniae and Acinetobacter sp.). We found that immunological-challenge larvae have stronger antimicrobial activity than the control groups. The Minimum Inhibitory Concentrations (MIC) for bacteria against for infected larvae were 12.5 mg/ml for MRSA, S. pyogenes, B. subtilis, M. luteus, E. coli, and K. pneumoniae. As for bactericidal activity, the MBC of E. coli infected larvae was 25mg/ml against S. pyogenes and B. subtilis. In conclusion, BSFL hemolymph has antibacterial activity against a range of bacteria and could be a candidate for novel antimicrobial development.","PeriodicalId":16968,"journal":{"name":"Journal of Pure and Applied Microbiology","volume":"10 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139198171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential of Animal Excreta as a Source of Probiotic Lactic Acid Bacteria for Aflatoxin B1 Detoxification by the Surface Binding Mechanism 动物排泄物作为益生乳酸菌来源的潜力，可通过表面结合机制对黄曲霉毒素 B1 进行解毒

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Journal of Pure and Applied Microbiology Pub Date : 2023-11-29 DOI: 10.22207/jpam.17.4.33

Chammakalayil Sukumaran Arun, Pambayan Ulagan Mahalingam, Haris Parengal, Joby Thomas

{"title":"Potential of Animal Excreta as a Source of Probiotic Lactic Acid Bacteria for Aflatoxin B1 Detoxification by the Surface Binding Mechanism","authors":"Chammakalayil Sukumaran Arun, Pambayan Ulagan Mahalingam, Haris Parengal, Joby Thomas","doi":"10.22207/jpam.17.4.33","DOIUrl":"https://doi.org/10.22207/jpam.17.4.33","url":null,"abstract":"Aflatoxins (AFs) are the most potent and ubiquitously found mycotoxins, capable of causing contamination in agricultural products. Aflatoxin B1 (AFB1) is the most toxic and primarily produced Aflatoxin and will be a real threat to the safety of food and feeds. The current study searched for the potential of Lactic acid bacteria (LAB) isolated from animal excreta for AFB1 mitigation. Three LAB out of 56 isolates were found to exhibit more than 50% sorbent action with AFB1 in phosphate-buffered saline (PBS) and were identified as Lactococcuslactis strain CF_6 (OP183481) (65.38%), Lacticaseibacillus casei strain CW_3 (OP183482)(52.63%) and Lactobacillus acidophilus strain CE_4 (OP183483)(63.13%). More than 60% of the total AFB1 removal was observed in 2 hr of incubation, and maximum sorbent action was found at a pH 6-7 range at 37oC for 24 hours. In the Scanning Electron Microscope (SEM) analysis, heat-killed cells showed a significant increase in cell surface binding area, which improved the surface binding for all isolates except L. casei strain CW_3; however, it proves that LAB surface binding is strain-specific rather than heat treatment. Moreover, the rise in AFB1 concentration improved the rate of the sorbent action but did not observe any substantial changes in total AFB1 detoxification. So, it is concluded that the animal excreta may be a versatile source of probiotic LAB for AFB1 detoxification by surface binding.","PeriodicalId":16968,"journal":{"name":"Journal of Pure and Applied Microbiology","volume":"24 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139210437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gut Microbial Profile Differences in Autoimmune Diseases 自身免疫性疾病的肠道微生物特征差异

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Journal of Pure and Applied Microbiology Pub Date : 2023-11-29 DOI: 10.22207/jpam.17.4.40

M. Alkhulaifi, Shahad A. Bin Selayem, Munirah A. Alquwayzani, Nujud A. Alabdullatif, Godfred A. Menezes

{"title":"Gut Microbial Profile Differences in Autoimmune Diseases","authors":"M. Alkhulaifi, Shahad A. Bin Selayem, Munirah A. Alquwayzani, Nujud A. Alabdullatif, Godfred A. Menezes","doi":"10.22207/jpam.17.4.40","DOIUrl":"https://doi.org/10.22207/jpam.17.4.40","url":null,"abstract":"The human gut microbiota has been widely studied due to the possibility of high-throughput sequencing. Humans are distinctly inhabited by normal flora and symbiotic microbial flora, with bacteria accounting for the vast bulk of the component microorganisms. These organisms can be found in a variety of locations throughout the body, including the oral cavity, vagina, skin and stomach. Microbe types and abundance vary in different organs of the same person, but they may also differ between persons. They are very important for human health and also affect the immune system by altering its metabolism and behavior. Conditions such as malnutrition, Crohn’s disease, inflammatory bowel disease and colon colitis, in addition to metabolic disorders including type II diabetes and obesity, have all been associated with the gut microbiota. Several studies in recent years have emphasized the relevance and involvement of commensal bacteria in the development of a variety of disorders, including autoimmune diseases. Autoimmune diseases, Such as Graves’ disease, systemic erythematosus lupus (SLE), and irritable bowel syndrome (IBS), are commonly known for their loss of self-tolerance, a hyperactive reaction against the body’s own tissue. Autoimmune diseases are triggered by the immune system targeting self-tissues, and their global frequency is estimated to be between 3 and 5%. This review reaffirms the links between autoimmune disorders and gut bacteria. The precise pathophysiology is unknown; however, environmental factors (such as lifestyle, diet, medications, and infections) and specific genetic conditions have been expected. The gut microbiota is important in autoimmunity because changes in microbial composition can trigger immunological tolerance loss.","PeriodicalId":16968,"journal":{"name":"Journal of Pure and Applied Microbiology","volume":"59 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139209578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of Antimicrobial Effectiveness of Orange and Lime’s Extracts on Aeromonas sobria and Aeromonas hydrophila 比较橙提取物和石灰提取物对嗜水气单胞菌和嗜水气单胞菌的抗菌效果

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Journal of Pure and Applied Microbiology Pub Date : 2023-11-29 DOI: 10.22207/jpam.17.4.38

N. D. A. Lubis, S. Amelia, Era Yusraini, Zikrina Rahmi, R. Balatif

{"title":"Comparison of Antimicrobial Effectiveness of Orange and Lime’s Extracts on Aeromonas sobria and Aeromonas hydrophila","authors":"N. D. A. Lubis, S. Amelia, Era Yusraini, Zikrina Rahmi, R. Balatif","doi":"10.22207/jpam.17.4.38","DOIUrl":"https://doi.org/10.22207/jpam.17.4.38","url":null,"abstract":"Aeromonas bacteria are often found in aquatic environments and can be isolated from various types of fish. Globally, the incidence of infection by Aeromonas reached 0.6–76 cases per 1 million people. Orange and lime are generally used as cooking spices to get rid of the fishy smell in fish, and also have antibacterial effects. The study compared the antibacterial effectiveness of lime and orange extracts on two Aeromonas species; A. sobria and A. hydrophila. Bacteria Aeromonas may emerge from fresh tuna bought in traditional markets. Lime and orange extracts were prepared using the maceration method using 96% methanol as solvent. The extract concentrations used were 6.25%, 12.5%, 25%, and 50%. Antimicrobial sensitivity test was carried out using the Kirby-Bauer method. The bacterial inhibition zone test revealed that the effectiveness of extracts on both oranges and limes was higher for the bacteria A. hydrophila compared to A. sobria. It is known from the average diameter of the inhibition zone, which is larger on A. hydrophila compared to A. sobria, except for the extract with a concentration of 6.25% orange and 50% lime. In a comparison between extracts, lime extract was found to be more effective as an antimicrobial than orange extract, except at a concentration of 6.5% in the bacterial test A. sobria and 50% concentration in the bacterial test A. hydrophila. In general, lime is more effective as an antimicrobial than orange.","PeriodicalId":16968,"journal":{"name":"Journal of Pure and Applied Microbiology","volume":"18 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139211142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phytochemical Analysis and Antibacterial Potential of Stevia rebaudiana (Bertoni, 1899) Leaf Extracts against Aeromonas Species: Influence of Extraction Methods and Solvents in Aquaculture Applications 甜叶菊（Stevia rebaudiana (Bertoni, 1899) ）叶提取物的植物化学分析和抗菌潜力：水产养殖应用中萃取方法和溶剂的影响

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Journal of Pure and Applied Microbiology Pub Date : 2023-11-29 DOI: 10.22207/jpam.17.4.31

Ashitha Raghu, Krishnakumar Velayudhannair

{"title":"Phytochemical Analysis and Antibacterial Potential of Stevia rebaudiana (Bertoni, 1899) Leaf Extracts against Aeromonas Species: Influence of Extraction Methods and Solvents in Aquaculture Applications","authors":"Ashitha Raghu, Krishnakumar Velayudhannair","doi":"10.22207/jpam.17.4.31","DOIUrl":"https://doi.org/10.22207/jpam.17.4.31","url":null,"abstract":"Recent studies have explored Stevia rebaudiana Bertoni leaf extracts for their antibacterial potential and phytochemical content. However, the impact of extraction methods and solvents on aquaculture bacteria remains understudied. This research aimed to evaluate the antibacterial, radical scavenging, and phytochemical properties of S. rebaudiana extracts against Aeromonas species. Dried S. rebaudiana leaves were extracted using methanol (Mt) and ethanol (Et) through Soxhlet and maceration methods (SMt, SEt, MMt and MEt respectively). Soxhlet extraction yielded higher amounts (36.29% for Mt, 23.87% for Et) compared to maceration. Phytochemical analysis identified phenolics, flavonoids, alkaloids, saponin, tannin, and steroids in all extracts. Notably, MEt had elevated phenolic and flavonoid content, while SEt contained more tannins. MEt exhibited the strongest antioxidant activity (IC50 = 67.95µg/mL), aligning with its high phenolic and flavonoid levels. In antibacterial assays against Aeromonas strains, ethanol extract showed the largest zone of inhibition (ZOI) of 16.67mm for A. salmonicida, followed by methanol extract (15mm) at 250 mg/mL, using maceration and Soxhlet methods, respectively. However, none of the extracts displayed activity against A. hydrophila. This suggests that cold maceration is a cost-effective method that preserves heat-sensitive secondary metabolites within a shorter extraction time. In conclusion, this study highlights the significance of extraction techniques and solvents in obtaining potent antibacterial and antioxidant extracts from S. rebaudiana leaves. The findings emphasize the potential of these extracts in aquaculture practices and open avenues for further research in utilizing natural compounds for sustainable aquaculture strategies.","PeriodicalId":16968,"journal":{"name":"Journal of Pure and Applied Microbiology","volume":"19 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139211774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rising Menace of Scrub Typhus - Current Status and Challenges 恙虫病日益严重的威胁--现状与挑战

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Journal of Pure and Applied Microbiology Pub Date : 2023-11-29 DOI: 10.22207/jpam.17.4.39

Diptimayee Rout, Ira Praharaj, S. Dalai, Swati Mishra, S. Otta

{"title":"Rising Menace of Scrub Typhus - Current Status and Challenges","authors":"Diptimayee Rout, Ira Praharaj, S. Dalai, Swati Mishra, S. Otta","doi":"10.22207/jpam.17.4.39","DOIUrl":"https://doi.org/10.22207/jpam.17.4.39","url":null,"abstract":"Scrub typhus, an age old disease, is caused by the intracellular bacterium Orientia tsutsugamushi. It has reemerged in recent years due to factors like climatic changes and human encroachment because of rampant urbanization. The disease is endemic in the area known as the ‘tsutsugamushi triangle’ and has recently spread its fangs into various other continents like South America and Africa. Although the disease is endemic in India, there is a lack of appropriate sero-epidemiology in community settings. It is one of the essential causes of acute undifferentiated fever in tropical locations and, if untreated, can cause mortality ranging from 2-30% of cases. Early diagnosis is an important parameter in administering the non beta-lactam regimen to prevent complications and mortality. Yet, there is a lack of accurate and rapid methods for diagnosis in the early stage of the disease, more so in rural areas where the disease is supposed to be predominant. The gold standard diagnostic test has its problems. Recently, there have been reports of drug resistance to the standard scrub typhus regimen. There is a gap of a decade in the research into this entity. Thus, a new look into the disease, its epidemiology and the challenges in its diagnostic scenario is an apt topic for discussion.","PeriodicalId":16968,"journal":{"name":"Journal of Pure and Applied Microbiology","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139214369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization, Characterization, and Cytotoxic Study of Bio Cellulose by Acetobacter sp Strains to Engender Biodegradable Food Wrapper 用醋酸纤维菌株制造生物可降解食品包装材料的生物纤维素优化、表征和细胞毒性研究

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Journal of Pure and Applied Microbiology Pub Date : 2023-11-29 DOI: 10.22207/jpam.17.4.32

S. Sri, Manjusha Varshini, M. Mekala, R. Ragunathan

{"title":"Optimization, Characterization, and Cytotoxic Study of Bio Cellulose by Acetobacter sp Strains to Engender Biodegradable Food Wrapper","authors":"S. Sri, Manjusha Varshini, M. Mekala, R. Ragunathan","doi":"10.22207/jpam.17.4.32","DOIUrl":"https://doi.org/10.22207/jpam.17.4.32","url":null,"abstract":"Acetic acid bacteria synthesized microbial cellulose were isolated from various citrus fruits, enabled by increased cellulose production to develop a biodegradable polymer as a food wrapper. The objective of the research cynosures on the isolation, enrichment, identification, and optimization of bacteria that produce cellulose, characterization, and cytotoxic study of the obtained cellulose. Two highly effective cellulose producers, Acetobacter lovaniensis (A1) and Acetobacter fabarum (A2), were isolated based on their morphology, biochemical analysis, and 16s rRNA sequencing. Studies were conducted to optimize pH, temperature, inoculum size, nitrogen, and carbon sources. Strain A1 produced 0.715 g/100 ml, whereas A2 produced 0.856 g/100 ml of cellulose under optimum growth conditions. The characteristics of microbial cellulose were examined using scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). A cytotoxicity study for the obtained cellulose has been conducted with mouse embryo fibroblast cells (3T3-L1) and showed 97% viability of cells with the lowest concentration of 12.5 µg/ml. These isolates could be employed in fermentation technology to produce cellulose polymer-based sustainable biodegradable food wrappers.","PeriodicalId":16968,"journal":{"name":"Journal of Pure and Applied Microbiology","volume":"303 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139209411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison between Quantitative Hepatitis B DNA and HBeAg Positivity to Detect Active Viral Replication among Patients with Hepatitis B infection in a Tertiary Care Hospital 比较定量乙型肝炎 DNA 与 HBeAg 阳性在一家三级医院乙型肝炎感染患者中检测活跃病毒复制的作用

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Journal of Pure and Applied Microbiology Pub Date : 2023-11-29 DOI: 10.22207/jpam.17.4.35

V. Preethi, S. Jaya Lakshmi, K. V. Leela

{"title":"Comparison between Quantitative Hepatitis B DNA and HBeAg Positivity to Detect Active Viral Replication among Patients with Hepatitis B infection in a Tertiary Care Hospital","authors":"V. Preethi, S. Jaya Lakshmi, K. V. Leela","doi":"10.22207/jpam.17.4.35","DOIUrl":"https://doi.org/10.22207/jpam.17.4.35","url":null,"abstract":"To study the correlation between quantitative Hepatitis B DNA and HBeAg positivity among HBsAg positive patients in a tertiary care hospital. To detect HBsAg, HBeAg levels by CMIA (Chemiluminescent microparticle immunoassay). To perform quantitative Hepatitis B virus DNA assay using Real Time Polymerase Chain Reaction. The study took place between July and December 2022 for 6 months. Sixty four patients were included in the study for whom HBsAg was positive by CMIA and for whom HBeAg (detected by CMIA) and Hepatitis B Virus DNA by quantitative Real Time PCR was performed. Of the 64 HBsAg reactive patients, the ‘e’ antigen of Hepatitis B virus was positive in six patients. In all the six HBeAg positive patients, HBV DNA was detected with a range of 78-10,288 IU/ml with 66% having levels more than 2000 IU/ml and requiring treatment. Among the 58 patients who were negative for the ‘e’ antigen of Hepatitis B virus, the Hepatitis B Viral DNA was detected in 27 patients with a range of 10-1,76,000 IU/ml, with 21% of patients having levels more than 2000 IU/ml. HBeAg is a good serological test to detect viral replication. However, it alone might not be sufficient as quantitative HBV DNA levels are more accurate and it does identify more patients who may require treatment.","PeriodicalId":16968,"journal":{"name":"Journal of Pure and Applied Microbiology","volume":"44 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139212032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Knowledge, Attitude and Practices Regarding Tuberculosis Infection Control Practices among Health Care Workers at Tertiary Care Centre, Rajnandgaon 拉杰南德贡三级医疗中心医护人员对结核病感染控制措施的认识、态度和做法

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Journal of Pure and Applied Microbiology Pub Date : 2023-11-29 DOI: 10.22207/jpam.17.4.34

Pawan Jethani, Surender Kaur, Prakash Khunte

{"title":"Knowledge, Attitude and Practices Regarding Tuberculosis Infection Control Practices among Health Care Workers at Tertiary Care Centre, Rajnandgaon","authors":"Pawan Jethani, Surender Kaur, Prakash Khunte","doi":"10.22207/jpam.17.4.34","DOIUrl":"https://doi.org/10.22207/jpam.17.4.34","url":null,"abstract":"Tuberculosis infectious disease owns highest burden of respiratory infection. India being the largest contributor to global Tuberculosis cases. Mycobacterium tuberculosis mode of transmission by respiratory route require airborne precautions to be strictly followed in healthcare settings to minimize the transmission. Research was plan to assess fundamentals of Tuberculosis infection control among healthcare workers. Questionnaire based cross-sectional study which included sociodemographic characteristics, with twenty-five questions on knowledge of Tuberculosis, attitude and practices for Tuberculosis infection control was conducted in Bharat Ratna Late Shri Atal Bihari Vajpayee Memorial Medical College, Rajnandgaon Chhattisgarh from April 2023 to May 2023. 156 HCWs with mean age group 36 ± 9.7 (18-59) years participated in the study. Healthcare workers included were nurses (55.1%) and technicians (44.8%). Knowledge and attitude score observed in our study is 90.3% and 92.3% levelled as good to moderate but at the same time good to moderate practice observed is 59.6%. Certain deficiencies regarding some areas of preventive measures of tuberculosis was observed among participants which require improvement by conducting training sessions.","PeriodicalId":16968,"journal":{"name":"Journal of Pure and Applied Microbiology","volume":"48 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139213344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adherence of Candida Species Isolated from Patients with Head and Neck Cancer Presented for Radiation Therapy and its Correlation with Hemogram 从接受放射治疗的头颈癌患者身上分离出的念珠菌菌种的依附性及其与血象图的相关性

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Journal of Pure and Applied Microbiology Pub Date : 2023-11-29 DOI: 10.22207/jpam.17.4.36

Neethu Babu, Chithralekha Saikumar, C. J. Raphael

{"title":"Adherence of Candida Species Isolated from Patients with Head and Neck Cancer Presented for Radiation Therapy and its Correlation with Hemogram","authors":"Neethu Babu, Chithralekha Saikumar, C. J. Raphael","doi":"10.22207/jpam.17.4.36","DOIUrl":"https://doi.org/10.22207/jpam.17.4.36","url":null,"abstract":"The objective of the current study was to quantify the adherence property of Candida species causing infections in head and neck cancer patients on radiation therapy (RT) or chemoradiotherapy (CRT). Additionally, the study aimed to establish a correlation between adherence properties and patients’ hemograms. A prospective cohort study was done on head and neck cancer (HNC) patients with oropharyngeal candidiasis. All patients received radiation doses ranging from 60 to 70 Gy using Intensity Modulated Radiation Therapy. Inoperable patients received concurrent chemotherapy with cisplatin 40mg/m2 once weekly during the course of RT. Swabs were collected from patients with signs and symptoms of candidiasis and microbiological confirmation and speciation were done according to standard protocol. An in vitro adherence test was done. Hemogram of all the patients at the start of infection was compared with the adherence. Candidiasis was detected in 58(35.3%) out of a total of 164 patients (Age 36-85). Candida albicans was the most prevalent 39(67.2%) species isolated. C.albicans showed the highest adherence which was statistically significant. The hemogram at the start of treatment and with C. albicans infection showed a statistically significant decrease in the absolute lymphocyte count (p-value 0.0001), RBC count (p-value 0.004) and haemoglobin level (p-value 0.003). Less adherence was seen with non-albicans Candida infection when compared to C.albicans (Odds 0.894, 95% CI -0.818-0.977), significant at 5 % (0.013). C. albicans showed significant adherence, which indicates the severity of infection. The occurrence of oral candidiasis is strongly associated with low lymphocyte count and severity of mucositis. Absolute lymphocyte count needs to be monitored in HNC patients who are undergoing RT or CRT.","PeriodicalId":16968,"journal":{"name":"Journal of Pure and Applied Microbiology","volume":"6 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139213698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0