甜叶菊(Stevia rebaudiana (Bertoni, 1899) )叶提取物的植物化学分析和抗菌潜力:水产养殖应用中萃取方法和溶剂的影响

IF 0.7 Q4 MICROBIOLOGY
Ashitha Raghu, Krishnakumar Velayudhannair
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引用次数: 0

摘要

最近的研究探索了甜叶菊叶提取物的抗菌潜力和植物化学成分含量。然而,萃取方法和溶剂对水产养殖细菌的影响仍未得到充分研究。本研究旨在评估 S. rebaudiana 提取物对气单胞菌的抗菌、自由基清除和植物化学特性。通过索氏提取法和浸渍法(分别为 SMt、SEt、MMt 和 MEt),使用甲醇(Mt)和乙醇(Et)提取干燥的 S. rebaudiana 叶子。与浸渍法相比,索氏提取的产量更高(Mt 为 36.29%,Et 为 23.87%)。植物化学分析确定了所有提取物中的酚类、黄酮类、生物碱、皂苷、单宁和类固醇。值得注意的是,MEt 的酚类和类黄酮含量较高,而 SEt 则含有较多的单宁。MEt 表现出最强的抗氧化活性(IC50 = 67.95µg/mL),这与其酚类和黄酮类化合物含量高是一致的。在针对气单胞菌株的抗菌试验中,采用浸渍法和索氏提取法,乙醇提取物对沙门氏菌的抑制面积(ZOI)最大,为 16.67 毫米,其次是甲醇提取物(15 毫米),抑制面积为 250 毫克/毫升。然而,没有一种提取物显示出对嗜水甲藻的活性。这表明冷浸渍法是一种经济有效的方法,能在较短的提取时间内保留对热敏感的次生代谢物。总之,本研究强调了萃取技术和溶剂在从 S. rebaudiana 叶子中获得强效抗菌和抗氧化萃取物方面的重要性。研究结果强调了这些提取物在水产养殖实践中的潜力,并为进一步研究利用天然化合物实现可持续水产养殖策略开辟了道路。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Phytochemical Analysis and Antibacterial Potential of Stevia rebaudiana (Bertoni, 1899) Leaf Extracts against Aeromonas Species: Influence of Extraction Methods and Solvents in Aquaculture Applications
Recent studies have explored Stevia rebaudiana Bertoni leaf extracts for their antibacterial potential and phytochemical content. However, the impact of extraction methods and solvents on aquaculture bacteria remains understudied. This research aimed to evaluate the antibacterial, radical scavenging, and phytochemical properties of S. rebaudiana extracts against Aeromonas species. Dried S. rebaudiana leaves were extracted using methanol (Mt) and ethanol (Et) through Soxhlet and maceration methods (SMt, SEt, MMt and MEt respectively). Soxhlet extraction yielded higher amounts (36.29% for Mt, 23.87% for Et) compared to maceration. Phytochemical analysis identified phenolics, flavonoids, alkaloids, saponin, tannin, and steroids in all extracts. Notably, MEt had elevated phenolic and flavonoid content, while SEt contained more tannins. MEt exhibited the strongest antioxidant activity (IC50 = 67.95µg/mL), aligning with its high phenolic and flavonoid levels. In antibacterial assays against Aeromonas strains, ethanol extract showed the largest zone of inhibition (ZOI) of 16.67mm for A. salmonicida, followed by methanol extract (15mm) at 250 mg/mL, using maceration and Soxhlet methods, respectively. However, none of the extracts displayed activity against A. hydrophila. This suggests that cold maceration is a cost-effective method that preserves heat-sensitive secondary metabolites within a shorter extraction time. In conclusion, this study highlights the significance of extraction techniques and solvents in obtaining potent antibacterial and antioxidant extracts from S. rebaudiana leaves. The findings emphasize the potential of these extracts in aquaculture practices and open avenues for further research in utilizing natural compounds for sustainable aquaculture strategies.
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来源期刊
Journal of Pure and Applied Microbiology
Journal of Pure and Applied Microbiology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-MICROBIOLOGY
CiteScore
2.00
自引率
0.00%
发文量
266
审稿时长
11 months
期刊介绍: Journal of Pure and Applied Microbiology (JPAM) is a peer-reviewed, open access international journal of microbiology aims to advance and disseminate research among scientists, academics, clinicians and microbiologists around the world. JPAM publishes high-quality research in all aspects of microbiology in both online and print form on quarterly basis.
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