Journal of Pure and Applied Microbiology最新文献_第2页

Microbial Induced Biotechnological Processes for Biofuel Production from Waste Organics Conversion 利用废弃有机物转化生产生物燃料的微生物诱导生物技术过程

IF 0.7

Journal of Pure and Applied Microbiology Pub Date : 2024-07-22 DOI: 10.22207/jpam.18.3.08

Sruthy MS Pillai, R. K. Srivastava, Sujeet Pratap Singh

{"title":"Microbial Induced Biotechnological Processes for Biofuel Production from Waste Organics Conversion","authors":"Sruthy MS Pillai, R. K. Srivastava, Sujeet Pratap Singh","doi":"10.22207/jpam.18.3.08","DOIUrl":"https://doi.org/10.22207/jpam.18.3.08","url":null,"abstract":"In the current era there are huge quantities of waste organic matter available, creating a big burden to the environment. To address these issues, researchers started to apply effective and microbial induced biotechnological processes that can mitigate these waste matters. In this context, different nature of microbial systems are involved in hydrolysing the waste organic material into fermentable sugar. These can be easily consumed by specific microbial systems like Saccharomyces cerevisiae MTCC 3821 and Clostridium acetobutylicum that produced bioethanol and biobutanol, respectively. Saccharomyces cerevisiae was cultured in specific media and incubated at rotary shaker with 150 rpm at 30°C for 72 to 96 hours. Ethanol concentrations from different waste matters were found in the range of 1.2-1.5 g.L-1. Ethanol synthesis was done by shake flask experiment with addition of glucose (50 g.L-1) to waste organic hydrolyzed solution. Non-glucose media produced less than 3 g.L-1 ethanol but glucose media produced 4.5 g.L-1. Next, Clostridium acetobutylicum was grown in culture media containing waste organics as sole carbon substrate with pH 7 and then was incubated in anaerobic conditions at 35°C for 72 hours, produced butanol (0.7 to 1.25 g.L-1). This research work promoted biofuels synthesis by keeping a waste mitigation strategy.","PeriodicalId":16968,"journal":{"name":"Journal of Pure and Applied Microbiology","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141814930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of Bioactive Compounds from Vegetable Waste using Microbial Consortia 利用微生物联合体从蔬菜废弃物中生产生物活性化合物

IF 0.7

Journal of Pure and Applied Microbiology Pub Date : 2024-07-22 DOI: 10.22207/jpam.18.3.15

Anita M. Chandak, S. A. Mankar, Rupali S. Balpande

{"title":"Production of Bioactive Compounds from Vegetable Waste using Microbial Consortia","authors":"Anita M. Chandak, S. A. Mankar, Rupali S. Balpande","doi":"10.22207/jpam.18.3.15","DOIUrl":"https://doi.org/10.22207/jpam.18.3.15","url":null,"abstract":"Utilizing vegetable waste to produce bioactive compounds through microbial consortia represents a holistic solution to waste management challenges. Efficient waste collection systems in major cities ensure proper segregation of vegetable waste and lay the groundwork for resource utilization. Segregating waste at its source enhances waste stream quality and facilitates downstream processing. Research and development efforts investigating tailored microbial consortia seek to optimize waste degradation and bioactive compound yields, thereby unlocking the potential of waste. This approach significantly reduces greenhouse gas emissions by diverting vegetable waste from landfills, thereby mitigating climate change effects and improving air quality. Moreover, it conserves natural resources by reducing the need for virgin materials and promoting biodiversity, conservation, and sustainable resource management. Here, the enzymatic activities and phenolic concentrations derived from the biodegradation of vegetable waste were analyzed over 14 days. Protease activity that plays a vital role in breaking down proteins reached a notable level of 300 µg/ml after 14-day incubation. Lipase activity which is essential for lipid breakdown was observed at a concentration of 6.3 µg/ml. Furthermore, phenolic concentration analysis revealed a significant range, with values ranging from 225 µg/ml to 240 µg/ml after 14 days of incubation. Phenolics are phytochemicals possessing antioxidant properties and potential health benefits. These findings provide valuable insight into the efficacy of utilizing vegetable waste as a substrate for enzymatic and phytochemical production. The observed enzymatic activities and phenolic concentrations highlight the potential of vegetable waste as a valuable resource for the sustainable production of industrially relevant compounds.","PeriodicalId":16968,"journal":{"name":"Journal of Pure and Applied Microbiology","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141815905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Therapeutic Efficacy of Baicalein Green Biomolecule in Methicillin-Resistant Staphylococcus aureus Murine Mastitis Model 黄芩苷绿色生物分子在耐甲氧西林金黄色葡萄球菌小鼠乳腺炎模型中的疗效

IF 0.7

Journal of Pure and Applied Microbiology Pub Date : 2024-07-12 DOI: 10.22207/jpam.18.3.04

Srishti Soni, Reena Mukherjee, Ujjwal Kumar De, Deeksha Bharti, Mamta Singh, B. R. Paul, Varun Kumar Sarkar, K. Sharun, N. Barkathullah, M. Saminathan

{"title":"Therapeutic Efficacy of Baicalein Green Biomolecule in Methicillin-Resistant Staphylococcus aureus Murine Mastitis Model","authors":"Srishti Soni, Reena Mukherjee, Ujjwal Kumar De, Deeksha Bharti, Mamta Singh, B. R. Paul, Varun Kumar Sarkar, K. Sharun, N. Barkathullah, M. Saminathan","doi":"10.22207/jpam.18.3.04","DOIUrl":"https://doi.org/10.22207/jpam.18.3.04","url":null,"abstract":"Methicillin-resistant Staphylococcus aureus (MRSA) mastitis poses a significant threat to dairy herds worldwide, given its resistance to methicillin and other β-lactam antibiotics, which often leads to treatment failure. Consequently, there is an urgent need for safe and effective alternative therapeutic approaches. Recent investigations have highlighted the potential of baicalein, a natural flavonoid known for its potent anti-inflammatory and antibacterial properties, especially its synergistic effects with β-lactam antibiotics against MRSA. However, the limited solubility and bioavailability of baicalein hinder its biomedical utility. The present study assessed the therapeutic efficacy of encapsulated baicalein in chitosan, forming a tricomplex with a β-lactam antibiotic, using a murine model of MRSA-induced mastitis. The experimental design comprised seven groups, each consisting of six mice. We evaluated the ability of various treatment regimens to mitigate histopathological alterations and bacterial burden induced by MRSA infection, aiming to elucidate underlying mechanisms. Our results revealed that tricomplex treatment significantly reduced bacterial load in mammary tissue and preserved tissue integrity, resulting in decreased inflammatory responses post-MRSA inoculation. In addition, tricomplex treatment markedly reduced mean leukocyte and neutrophil counts in blood and suppressed the matrix metalloproteinase-9 (MMP-9) concentration and C-reactive protein (CRP) response. Notably, the synergistic interaction between baicalein and amoxicillin was particularly pronounced. Our findings suggest that chitosan-encapsulated baicalein combined with a β-lactam antibiotic holds promise as a therapeutic option for MRSA-induced mastitis. Further investigations, particularly in target animal species, are warranted to comprehensively evaluate its clinical feasibility.","PeriodicalId":16968,"journal":{"name":"Journal of Pure and Applied Microbiology","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141652403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of Robust Anti-SARS-CoV-2 Trajectories among Communities with and without COVID-19 Infection 感染 COVID-19 和未感染 COVID-19 的社区之间的抗 SARS-CoV-2 强健轨迹关联

IF 0.7

Journal of Pure and Applied Microbiology Pub Date : 2024-07-12 DOI: 10.22207/jpam.18.3.05

Balamurali Venkatesan, L. Vajravelu, S. Ravi, Jayaprakash Thulukanam, Om Lokesh Muthamilan

{"title":"Association of Robust Anti-SARS-CoV-2 Trajectories among Communities with and without COVID-19 Infection","authors":"Balamurali Venkatesan, L. Vajravelu, S. Ravi, Jayaprakash Thulukanam, Om Lokesh Muthamilan","doi":"10.22207/jpam.18.3.05","DOIUrl":"https://doi.org/10.22207/jpam.18.3.05","url":null,"abstract":"Globally, 767 million people have been affected by SARS-CoV-2 infection and nearly a population of 6.94 million were deceased as per the World Health Organization (WHO) as of June 7, 2023. In India, the spreading of infections is now being restricted by the use of BBV-152 (CovaxinTM) and ChAdOx1-nCOV (CovishieldTM) vaccines. The present study comprises 327 candidates and Chemiluminescent microparticle immunoassay (CMIA) was used as a quantitative analytical tool to detect IgG and IgM antibodies. Out of 327 candidates, 177 (54.1%) were vaccinated and 150 (45.9%) were non-vaccinated. Among vaccinated and non-vaccinated candidates, 49 (27.7%%) and 44 (29.3%) had a history of infection, respectively; meanwhile, most of the study participants were immunized with CovishieldTM (n=145, 81.9%) and 18.1% (n=32) were immunized with CovaxinTM. There were insignificant differences observed among immunized as well as non-immunized study participants in considering median age, gender, age categories, IgM levels, or IgM seropositivity. Predictably, there was an important variation in IgG median values and IgG positivity noticed among the immunized and non-immunized categories as well as between populations with and without preceding infections. Our research is hence coherent with prospective requirements for booster shots to assist in controlling the rate of infections and fatality rates together throughout the pandemic conditions.","PeriodicalId":16968,"journal":{"name":"Journal of Pure and Applied Microbiology","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141654062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High Burden of Carbapenem-Resistant Organisms Screened by Xpert Carba-R Assay Versus Culture from Intensive Care Patients in Indonesia 印度尼西亚重症监护患者中经 Xpert Carba-R 检测法与培养法筛查出的耐碳青霉烯类病菌的高负担率

IF 0.7

Journal of Pure and Applied Microbiology Pub Date : 2024-07-12 DOI: 10.22207/jpam.18.3.03

Mohammad Agung Marzah, F. S. Widyatama, Rosantia Sarassari, W. Setyarini, Alicia Margaretta Widya, Sulikah Sulikah, B. Semedi, P. Airlangga, K. Kuntaman, Toshiro Shirakawa, E. Koendhori

{"title":"High Burden of Carbapenem-Resistant Organisms Screened by Xpert Carba-R Assay Versus Culture from Intensive Care Patients in Indonesia","authors":"Mohammad Agung Marzah, F. S. Widyatama, Rosantia Sarassari, W. Setyarini, Alicia Margaretta Widya, Sulikah Sulikah, B. Semedi, P. Airlangga, K. Kuntaman, Toshiro Shirakawa, E. Koendhori","doi":"10.22207/jpam.18.3.03","DOIUrl":"https://doi.org/10.22207/jpam.18.3.03","url":null,"abstract":"The World Health Organization (WHO) considers carbapenem-resistant organisms (CROs) to be critical-level pathogens. Regular screening for high-risk CRO colonization is essential, especially in the ICU. Direct detection of carbapenem-resistant genes is possible using the FDA-approved Xpert Carba-R assay. This study evaluated its reliability compared with the culture technique at a tertiary hospital in Indonesia. A high number of CRO colonization was found using the culture technique and the Xpert Carba-R assay with about 31 and 26 positive results out of 100 total samples, respectively. Both methods detected blaNDM in 11 samples, and the Xpert Carba-R assay detected one sample co-presenting with blaVIM that was not detected by PCR. The Xpert Carba-R assay did not detect the gene in 73 samples following negative results with the culture technique. Fifteen samples were detected gene by the Xpert Carba-R assay though there was no gene by the culture method, showing that the Xpert Carba-R assay demonstrated a high degree of sensitivity in identifying carbapenem-resistance genes. Carbapenem-resistance genes common in Indonesia other than those examined by Xpert Carba-R assay in this study (i.e., blaOXA-23 and blaOXA-24) or non-enzymatic mechanisms may also produce resistance in many colonies without the examined genes. Finally, the Xpert Carba-R assay produced faster findings than the culture technique.","PeriodicalId":16968,"journal":{"name":"Journal of Pure and Applied Microbiology","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141653100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diagnosis of Ventilator-Associated Pneumonia – A Systematic Review and Meta-analysis of Laboratory Techniques 呼吸机相关肺炎的诊断 - 实验室技术的系统回顾和元分析

IF 0.7

Journal of Pure and Applied Microbiology Pub Date : 2024-07-12 DOI: 10.22207/jpam.18.3.02

H. K. Thakur, B. Tarai, Aradhana Bhargava, Sonu K. Agarwal, Pankaj Soni, Sudhakar Kancharla, Prachetha Kolli, Gowtham Mandadapu, M. Jena

{"title":"Diagnosis of Ventilator-Associated Pneumonia – A Systematic Review and Meta-analysis of Laboratory Techniques","authors":"H. K. Thakur, B. Tarai, Aradhana Bhargava, Sonu K. Agarwal, Pankaj Soni, Sudhakar Kancharla, Prachetha Kolli, Gowtham Mandadapu, M. Jena","doi":"10.22207/jpam.18.3.02","DOIUrl":"https://doi.org/10.22207/jpam.18.3.02","url":null,"abstract":"Hospitalized patients on mechanical ventilation are at high-risk of developing ventilator-associated pneumonia (VAP), making early and accurate diagnosis essential for the best possible treatment outcomes. This review examined various laboratory techniques, such as aerobic cultures, Gram’s stain, and molecular techniques, to assess how well they diagnose VAP. We have done search strategies using Google Scholar, Medline Complete, and PubMed. Extensive statistical tools were utilized to examine studies and evaluate the diagnostic accuracy of laboratory results. Multiplex PCR was superior to Gram’s stain and culture methods in terms of sensitivity (92%) and specificity (86%). On the other hand, Gram’s stain showed the highest specificity (78.9%) and the lowest sensitivity (74.6%). The results of semi-quantitative, quantitative, enrichment culture showed a lower specificity (75.97%) but a moderate sensitivity (78.5%). The most accurate diagnostic approach for VAP, according to a meta-analysis, was multiplex PCR-based testing, closely followed by culture methods. Beyond separate tests, multiplex PCR, culture, and Gram’s stain combination increased sensitivity. Prompt and precise VAP diagnosis is essential for efficient treatment. With possible improvements through combination diagnostic techniques, multiplex PCR remains the most accurate diagnostic tool. However, more investigation is necessary to improve and certify VAP diagnostic instruments.","PeriodicalId":16968,"journal":{"name":"Journal of Pure and Applied Microbiology","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141654315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Understanding the Pathogenesis of Endothelial Injury Induced by Bluetongue Virus in Experimentally Infected Sheep 了解蓝舌病病毒诱发实验感染绵羊内皮损伤的发病机制

IF 0.8

Journal of Pure and Applied Microbiology Pub Date : 2024-06-16 DOI: 10.22207/jpam.18.3.01

Deepti Singh, M. Saminathan, M. Dinesh, S. Pavulraj, A.A.P. Milton, K. Dhama, D. Ranjith, G.K. Sharma, Ayushi Singh, K.P. Singh

{"title":"Understanding the Pathogenesis of Endothelial Injury Induced by Bluetongue Virus in Experimentally Infected Sheep","authors":"Deepti Singh, M. Saminathan, M. Dinesh, S. Pavulraj, A.A.P. Milton, K. Dhama, D. Ranjith, G.K. Sharma, Ayushi Singh, K.P. Singh","doi":"10.22207/jpam.18.3.01","DOIUrl":"https://doi.org/10.22207/jpam.18.3.01","url":null,"abstract":"Bluetongue (BT) is an economically important contagious viral disease transmitted by insect vectors of Culicoides species affecting both domestic and wild ruminants caused by Genus Orbivirus of Reoviridae family. Present in vivo study for the first time conducted to understand the role of oxidative stress and acute phase proteins in the pathogenesis of endothelial injury in target blood vessels of BTV-4 infected sheep. Twelve sheep of either sex were divided into two groups of six animals each and infected intradermally at multiple sites in the neck region with 6×105.9 TCID50/ml of BTV-4 and animals of control group were inoculated with uninfected tissue culture fluid. Animals were sacrificed at 3, 7, 15, 28, 60, and 90 days post infection (dpi) and postmortem examination was conducted. Oxidative stress parameters like LPO, SOD, GPO and catalase in the blood vessels, hemolysate and serum were estimated. Oxidative stress parameter like LPO was upregulated and anti-oxidant parameters like SOD and GPO were downregulated in blood vessels homogenates, hemolysate and serum of BTV-4 infected animals than uninfected control. This shows the role of oxidative stress in causing endothelial cell damage during BTV infection. Acute phase proteins like CRP and fibrinogen in serum and plasma were estimated and found upregulated in BTV-4 infected animals, which shows that inflammatory changes during the disease causes vascular damage. Prothrombin time was estimated in plasma of BTV-4 infected animals and was prolonged in BTV infected animals indicating coagulation disorder. Increased oxidative stress and fibrinogen, and prolonged prothrombin time indicated the coagulation disorders induced by BTV infection by causing endothelial cell damage and severe vascular disease results in congestion, haemorrhages and edema in the blood vessels and other target organs in sheep.","PeriodicalId":16968,"journal":{"name":"Journal of Pure and Applied Microbiology","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141335495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Parasporins of Bacillus thuringiensis Strain Exhibit Apoptosis-Mediated Selective Cytotoxicity to MDA-MB-231 Cells through Oxidative Stress 苏云金芽孢杆菌菌株的寄生菌素通过氧化应激对 MDA-MB-231 细胞产生凋亡介导的选择性细胞毒性

IF 0.8

Journal of Pure and Applied Microbiology Pub Date : 2024-06-02 DOI: 10.22207/jpam.18.2.51

Mohmmed M. Aljeldah, T. A. El-Kersh, Mourad A.M Aboul-Soud

{"title":"Parasporins of Bacillus thuringiensis Strain Exhibit Apoptosis-Mediated Selective Cytotoxicity to MDA-MB-231 Cells through Oxidative Stress","authors":"Mohmmed M. Aljeldah, T. A. El-Kersh, Mourad A.M Aboul-Soud","doi":"10.22207/jpam.18.2.51","DOIUrl":"https://doi.org/10.22207/jpam.18.2.51","url":null,"abstract":"Historically, the most important source of both antibiotics and anticancer medications has been microorganisms. Bacillus thuringiensis (Bt) is one of the most prominent bacterial species used as a therapeutic agent targeting cancerous cells in recent worldwide investigations. This study was designed to isolate, molecularly identify, and discover novel Saudi Arabian Bt strains that selectively exhibit cytotoxic properties against MDA-MB-231, a human triple-negative breast cancer (TNBC) cell model. The bacterial strain under investigation was biochemically typed using API 20E and API CH50 and molecularly typed using 16S rDNA sequencing. Flow cytometry and immunoblotting were performed to elucidate the mechanism-of-action (MOA). Molecular typing confirmed the identity of the isolated non-hemolytic strain to be Bt and was named Bt HAU-145. Microscopic examination showed that the strain possessed a parasporal (PS) crystal protein with a spherical morphology. Data of cytotoxicity assay based on MTT revealed that Bt HAU-145 strain exhibited selective and potent cytotoxicity against MDA-MB-231, with a 50 percent inhibition (IC50) of 28 µg/ml. FACS analysis revealed that PS proteins induced both late and early apoptosis in a ROS-dependent manner. Immunoblotting assays showed increased expression of caspase-3 in response to PS treatment, paralleled by a reduction in Bcl-2 expression. This is the first study to investigate the MOA of PS proteins from the Saudi Arabian Bt strain, showing an induction of apoptosis through a ROS-dependent mechanism in TNBC cells. It is hoped that PS-based therapeutic strategies will be investigated at the preclinical scale in non-human primates prior to the clinical scale in randomized clinical trials.","PeriodicalId":16968,"journal":{"name":"Journal of Pure and Applied Microbiology","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141273132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nasal Carriage and Antimicrobial Susceptibility Pattern of Staphylococcus aureus among Breastfeeding Mothers and their Infants 母乳喂养的母亲及其婴儿的金黄色葡萄球菌鼻腔携带情况和抗菌药敏感性模式

IF 0.8

Journal of Pure and Applied Microbiology Pub Date : 2024-06-02 DOI: 10.22207/jpam.18.2.54

Rike Syahniar, Adinta Anandani, Aning Subiyatin, Hanif Ahmad Mubarok

{"title":"Nasal Carriage and Antimicrobial Susceptibility Pattern of Staphylococcus aureus among Breastfeeding Mothers and their Infants","authors":"Rike Syahniar, Adinta Anandani, Aning Subiyatin, Hanif Ahmad Mubarok","doi":"10.22207/jpam.18.2.54","DOIUrl":"https://doi.org/10.22207/jpam.18.2.54","url":null,"abstract":"Most S. aureus infections are multidrug resistant. S. aureus infections often occur with prolonged conditions, causing increased treatment costs and mortality rates. There is a need to understand the antibiotic susceptibility pattern to S. aureus in mothers and infants because the burden of S. aureus infection in infants is high. This study aims to determine the prevalence of nasal carriers of S. aureus in lactating mothers and their infants and their antibiotic susceptibility patterns. This cross-sectional study involved 59 pairs of breastfeeding mothers and infants aged 0 to 6 months. The research was conducted in the work area of the South Tangerang City Health Office. We take a nasal swab of the mother and the baby. We used Vitek-2 to determine antibiotic resistance against S. aureus. Overall, we found 22/59 (37%) S. aureus in infants’ noses, 18% of whom were MRSA. In mothers, we found 18/59 (30%) S. aureus isolates and 17% were MRSA. The majority of S. aureus isolates from infants were sensitive to cefoxitin (82%), gentamicin (86%), ciprofloxacin (91%), levofloxacin (95%), moxifloxacin (91%), vancomycin (100%), clindamycin (82%), erythromycin (86%), nitrofurantoin (100%), linezolid (100%) and tetracycline (77%). The majority of S. aureus isolates from mothers are sensitive to cefoxitin (83%), gentamicin (94%), ciprofloxacin (89%), levofloxacin (89%), moxifloxacin (89%), vancomycin (100%), clindamycin (89%), erythromycin (89%), nitrofurantoin (100%), linezolid (100%) and tetracycline (83%). MRSA monitoring of mothers and babies in the community needs to be done to prevent and control the spread.","PeriodicalId":16968,"journal":{"name":"Journal of Pure and Applied Microbiology","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141273284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of Rickettsial Outer Membrane Protein A in the Pathogenesis of Rickettsial Diseases 立克次体外膜蛋白 A 在立克次体病发病机制中的作用

IF 0.8

Journal of Pure and Applied Microbiology Pub Date : 2024-06-02 DOI: 10.22207/jpam.18.2.55

Noor Hanisa Harun, M. Y. Yuhana, A. Chua, Seok Mui Wang

{"title":"Role of Rickettsial Outer Membrane Protein A in the Pathogenesis of Rickettsial Diseases","authors":"Noor Hanisa Harun, M. Y. Yuhana, A. Chua, Seok Mui Wang","doi":"10.22207/jpam.18.2.55","DOIUrl":"https://doi.org/10.22207/jpam.18.2.55","url":null,"abstract":"Diseases caused by Rickettsiales are often overlooked, although they pose important public health concerns. The Rickettsiales family comprises a broad range of intracellular bacteria with distinct evolutionary adaptations, making the development of treatment measures to combat infections, such as vaccines or antibiotics, a challenge. Interestingly, the outer membrane protein A (OmpA) was found to exist in the cell surface of most human pathogenic bacteria in the order Rickettsiales. However, knowledge about OmpA in each species and strain is scattered and ambiguous. In this study, we systematically compiled the existing information on OmpA and its relationship with human pathogenic rickettsiae to serve as a reference for future research. A comprehensive literature search was conducted using specific keywords across five databases. According to the literature, OmpA of spotted fever group rickettsia plays a crucial role as an adhesin and invasin that directly interacts with the surface of mammalian host cells to mediate bacterial localization in host cells. The presence of a premature stop codon in the amino acid sequence resulted in the secretion of non-functional OmpA, which is one of the main reasons for rickettsial strains or species to become avirulent. Similarly, OmpA also functions as an important adhesin in the Anaplasma family when it interacts with the sLex and sLex-like glycan of myeloid and endothelial cells, respectively. However, the OmpA of Anaplasma must be co-functional with the other two adhesins to promote bacterial internalization. Interestingly, certain sites in the amino acid residues of Ehrlichia and Orientia OmpA are predicted to be homologous to the binding domain region of Anaplasma OmpA. It is therefore suggested that OmpA is an important adhesin for bacteria to bind to their specific mammalian host cells.","PeriodicalId":16968,"journal":{"name":"Journal of Pure and Applied Microbiology","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141273265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0