Journal of Radiation Research and Applied Sciences最新文献

{"title":"Altered brain activation patterns in dyssynergic defecation: Investigating neural mechanisms through task-based fMRI analysis","authors":"Sheng-fu Zhang ,&nbsp;Li Wang ,&nbsp;Tong Zeng ,&nbsp;He-qiang Li ,&nbsp;Qi-xin Tan ,&nbsp;Fang Hu ,&nbsp;Tao Wu ,&nbsp;Shi-jun Qiu","doi":"10.1016/j.jrras.2026.102201","DOIUrl":"10.1016/j.jrras.2026.102201","url":null,"abstract":"<div><h3>Background</h3><div>Dyssynergic Defecation (DD) is a functional disorder characterized by paradoxical contraction or insufficient relaxation of the pelvic floor muscles during defecation. While behavioral and physiological factors have been extensively investigated, the underlying central neural mechanisms remain inadequately understood. This study aims to elucidate the differences in brain activation patterns between individuals with DD and healthy controls (HC) during a simulated defecation task utilizing fMRI, thereby addressing the limited comprehension of its neural mechanisms.</div></div><div><h3>Methods</h3><div>We recruited eight patients diagnosed with DD and ten HC participants. All subjects underwent task-based fMRI while engaging in a validated simulated defecation paradigm. Additionally, the Constipation Scoring System (CSS), the Patient Assessment of Constipation Quality of Life (PAC-QOL), and Montreal Cognitive Assessment (MOCA) were utilized to assess Clinical indicators. The fMRI data were preprocessed and analyzed employing a general linear model (GLM) to identify brain regions exhibiting significant activation during the task. Comparisons between groups were conducted, along with correlation analyses with Clinical indicators.</div></div><div><h3>Results</h3><div>The DD cohort displayed diminished activation in the right thalamus and right cerebellum (VI), whereas the HC group exhibited activation in the right cerebellum (VI) and the right inferior parietal lobule. Notably, the DD group demonstrated negative activation in several brain areas, including the left fusiform gyrus and left parahippocampal gyrus, when compared to HC. Correlation analysis indicated positive relationships between brain activation in specific regions, such as the right cerebellum (VI) and the left cerebellum (III), with CSS scores and symptoms of straining and incomplete evacuation.</div></div><div><h3>Conclusions</h3><div>Individuals with DD manifest a distinct profile of dysfunctional brain activation during simulated defecation. These findings suggest a central neural pathophysiology characterized by impaired sensorimotor integration, excessive cognitive inhibition, and altered interoceptive processing. This provides a novel perspective for comprehending DD and may guide future neuromodulation-based therapeutic approaches.</div></div>","PeriodicalId":16920,"journal":{"name":"Journal of Radiation Research and Applied Sciences","volume":"19 1","pages":"Article 102201"},"PeriodicalIF":2.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146077266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic integration and functional validation reveal PLAC8, CLCA4, and ADAMDEC1 as key biomarkers linking periodontitis and colorectal cancer","authors":"Ermei Peng ,&nbsp;Shaonan Hu ,&nbsp;Shuqin Liu","doi":"10.1016/j.jrras.2026.102206","DOIUrl":"10.1016/j.jrras.2026.102206","url":null,"abstract":"<div><h3>Objective</h3><div>By integrating transcriptomic datasets and performing subsequent laboratory validation, this research sought to uncover molecular markers shared by periodontitis and colorectal cancer.</div></div><div><h3>Methods</h3><div>Transcriptomic sequencing was performed on periodontitis and colorectal cancer tissues versus normal controls (periodontitis: n = 12 diseased vs. n = 12 healthy controls; colorectal cancer: n = 15 tumor samples vs. n = 15 matched adjacent normal tissues), using the Illumina NovaSeq 6000 platform with 150-bp paired-end sequencing. Differentially expressed genes (DEGs) underwent GO and KEGG enrichment analysis. Weighted gene co-expression network analysis (WGCNA) identified disease-associated modules. Random forest modeling and Venn analysis determined overlapping biomarkers. Experimental validation used qRT-PCR, CCK-8 viability assays, and colony formation assays in relevant cell lines.</div></div><div><h3>Results</h3><div>Functional analysis revealed both diseases were enriched in immune-inflammatory pathways, including cytokine signaling, TNF and NF-κB pathways. WGCNA identified distinct gene co-expression modules. Random forest analysis identified PLAC8, CLCA4, SEMA6D, CHP2, and ADAMDEC1 as top predictive features. Cross-disease analysis revealed seven shared genes. Experimental assays demonstrated a significant increase in PLAC8 and ADAMDEC1 expression, along with a marked reduction in CLCA4 under disease conditions (P &lt; 0.001). Functional studies demonstrated that modulating these genes had a significant impact on cell viability and colony formation (P &lt; 0.001).</div></div><div><h3>Conclusion</h3><div>This study identifies shared immune-inflammatory mechanisms between periodontitis and colorectal cancer, with PLAC8, CLCA4, and ADAMDEC1 as common biomarkers, providing insights into disease linkage and potential therapeutic targets.</div></div>","PeriodicalId":16920,"journal":{"name":"Journal of Radiation Research and Applied Sciences","volume":"19 1","pages":"Article 102206"},"PeriodicalIF":2.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146077839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing tote transfer cycle time in a gamma irradiator via pneumatic system redesign: A case study of IGMP Indonesia","authors":"Ari Satmoko,&nbsp;Roziq Himawan,&nbsp;Djarot Sulistio Wisnubroto,&nbsp;Mohammad Dhandhang Purwadi,&nbsp;Ferly Hermana,&nbsp;Budi Santoso,&nbsp;Agus Cahyono,&nbsp;Jos Budi Sulistyo,&nbsp;Hasriyasti Saptowati,&nbsp;Puji Santosa,&nbsp;Totti Tjiptosumirat,&nbsp;Dede Sutarya,&nbsp;Sunarto Sunarto,&nbsp;Agus Sumaryanto,&nbsp;Bang Rozali,&nbsp;Kristedjo Kurnianto,&nbsp;Jaja Sukmana","doi":"10.1016/j.jrras.2026.102180","DOIUrl":"10.1016/j.jrras.2026.102180","url":null,"abstract":"<div><div>A low-cost mechanical–control retrofit for the Merah Putih Gamma Irradiator (IGMP) is presented, aimed at shortening tote-transfer cycle time and improving simulated dose uniformity without altering the source or shielding. The intervention simplifies the pneumatic sequence from nine to six stages and slightly reassigns two tote positions to preserve path symmetry relative to the ⁶⁰Co racks. Based on operational logs and prototype testing, the average cycle time decreased from ∼65.3 s to ∼43.2 s per cycle (≈33.7 %), reducing the minimum batch duration from ∼78 to ∼50 min and widening the operating window for low-dose services (e.g., phytosanitary treatment, sprout inhibition). To evaluate potential dose effects, a comparative MicroShield v7 calculation was performed across all 72 irradiation positions on two quasi-2D planes. The simulated range narrowed primarily by lifting the minimum from 91.98 mSv to 130.86 mSv, while the peak remained at 1050.34 mSv. Consequently, the Dose Uniformity Ratio (DUR) improved from 11.4 to 8.0, indicating better homogeneity. Position-level analysis shows tote #37 increasing from 91.98 mSv to 131.04 mSv after reassignment, whereas tote #55 remains essentially unchanged (∼145.69 mSv). Assuming total simulated dose is represented by sampled points, the 72-cycle total rises from 32,896.96 mSv to 32,935.02 mSv (≈+0.12 %). Limitations: the six-stage sequence has not yet been deployed in production; results are based on full-scale prototype testing and simulations using a free-field approximation (no bunker scatter). Therefore, validation by field dose mapping under production conditions is required. Overall, the retrofit offers a transferable sequencing framework for tote-based irradiators in resource-constrained settings.</div></div>","PeriodicalId":16920,"journal":{"name":"Journal of Radiation Research and Applied Sciences","volume":"19 1","pages":"Article 102180"},"PeriodicalIF":2.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146077841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ARHGAP11A promotes proliferation and invasion through RhoA/RAC1 in LUAD","authors":"Xinyu Liu ,&nbsp;Xuesong Zhao ,&nbsp;Haifeng Hu ,&nbsp;Tianyu Zhang ,&nbsp;Yi Qi ,&nbsp;Bo Liu ,&nbsp;Bing Lan ,&nbsp;Bin Li","doi":"10.1016/j.jrras.2025.102123","DOIUrl":"10.1016/j.jrras.2025.102123","url":null,"abstract":"<div><h3>Background</h3><div>Lung adenocarcinoma (LUAD) ranks among the most common subtypes of lung cancer, characterized by a poor prognosis due to the scarcity of effective treatment options. Functional role and underlying mechanisms of Rho GTPase-activating protein 11A (ARHGAP11A) in LUAD remain insufficiently characterized. The study aims to explore it, with the hypothesis that ARHGAP11A is a pivotal driver of tumor progression and a potential therapeutic target.</div></div><div><h3>Methods</h3><div>The research employed bioinformatics analysis to identify ARHGAP11A's overexpression in LUAD and its correlation with poor prognosis. To evaluate how reducing ARHGAP11A affects LUAD cell movement and invasion, various functional tests such as cell scratch and transwell cell invasion assays were conducted. Additionally, the study investigated ARHGAP11A's involvement in LUAD epithelial-mesenchymal transition and its regulation through the RhoA/RAC1 pathway using RAC1-specific inhibitor EHT-1864. In vivo tumor growth was assessed following ARHGAP11A knockdown, and the efficacy of the RAC1-specific inhibitor EHT-1864 in reducing tumor growth was tested.</div></div><div><h3>Results</h3><div>The bioinformatics investigation indicated that ARHGAP11A is upregulated in LUAD and correlates with unfavorable outcomes. Cell migration and invasion were inhibited after ARHGAP11A knockdown. The study also implicated ARHGAP11A in LUAD epithelial-mesenchymal transition and demonstrated its regulation through the RhoA/RAC1 pathway using a RAC1-specific inhibitor. In vivo tumor growth was significantly inhibited by ARHGAP11A knockdown, and treatment with EHT-1864 reduced tumor growth, indicating a potential therapeutic strategy.</div></div><div><h3>Conclusion</h3><div>The study's findings establish ARHGAP11A as a key factor in LUAD progression, enhancing cell proliferation and invasion. These results warrant further exploration of ARHGAP11A's mechanisms and clinical applications, potentially leading to new therapeutic strategies for LUAD treatment.</div></div>","PeriodicalId":16920,"journal":{"name":"Journal of Radiation Research and Applied Sciences","volume":"19 1","pages":"Article 102123"},"PeriodicalIF":2.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145787132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A modified sine Weibull distribution with applications to finance and radiation sectors","authors":"Yen Liang Tung ,&nbsp;Gaber Sallam Salem Abdalla ,&nbsp;M.M. Abd El-Raouf ,&nbsp;M.A. El-Qurashi","doi":"10.1016/j.jrras.2025.102088","DOIUrl":"10.1016/j.jrras.2025.102088","url":null,"abstract":"<div><div>We present a novel approach that utilizes a familiar trigonometric function with the aim of enhancing the descriptions of real-world events. The presented method mainly relies on the sine function and is henceforth referred to as the modified sine-<span><math><mi>G</mi></math></span> (MS-<span><math><mi>G</mi></math></span>) approach. The MS-<span><math><mi>G</mi></math></span> method updates the data analysis tools by providing a more precise representation of practical data sets. In our case study, we utilize the MS-<span><math><mi>G</mi></math></span> method on the Weibull distribution leading to a new model termed the modified sine-Weibull (MS-Weibull) distribution. We investigate different mathematical characteristics of the MS-Weibull distribution, with particular emphasis on its quantile function, which is crucial for generating random variates and defining quartiles as well as percentiles We also carry out the mathematical derivations of point estimators for the MS-Weibull distribution. The derived estimators are further evaluated through simulation studies under various parameter values. To validate the MS-Weibull distribution, we analyze two data sets, taken from the finance and radiation fields. Our investigations using numerical and visual methods show that the MS-Weibull distribution repeatedly offers the best results based on specific criteria for assessing the goodness of fit of the model to the data.</div></div>","PeriodicalId":16920,"journal":{"name":"Journal of Radiation Research and Applied Sciences","volume":"19 1","pages":"Article 102088"},"PeriodicalIF":2.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145683061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Piezo1 drives microgravity-induced ANP secretion via calcium-JNK/c-Jun signaling in primary rat atrial cardiomyocytes","authors":"Jiao Sun ,&nbsp;Nana Zhu ,&nbsp;Tianlun Li ,&nbsp;Jianping Guo ,&nbsp;Hongtao Yuan ,&nbsp;Zhaoliang Shan","doi":"10.1016/j.jrras.2025.102074","DOIUrl":"10.1016/j.jrras.2025.102074","url":null,"abstract":"<div><h3>Background</h3><div>Weightlessness exposure during spaceflight induces cardiovascular deconditioning, including orthostatic intolerance (PSOI) upon re-exposure to gravity, partly attributed to dysregulated atrial natriuretic peptide (ANP) secretion. While ANP elevation occurs acutely in microgravity, the upstream mechanosensitive pathways regulating this response remain poorly understood. This study investigated the role of the mechanosensitive ion channel Piezo1 and the JNK/c-Jun signaling pathway in ANP regulation under simulated weightlessness.</div></div><div><h3>Methods</h3><div>Primary rat atrial cardiomyocytes were exposed to simulated microgravity (SM) using a rotary cell culture system. Experimental groups included: Control, SM, SM treated with the stretch-activated channel (SAC) inhibitor streptomycin (STR), SM treated with the JNK inhibitor SP600125, SM with Piezo1 knockdown via lentiviral shRNA (performed prior to SM exposure), and normal gravity treated with the Piezo1 agonist Yoda1. ANP expression, JNK/c-Jun pathway activation, Piezo1 expression, and intracellular calcium levels were assessed using Western blot, RT-PCR, and flow cytometry.</div></div><div><h3>Results</h3><div>SM significantly increased ANP expression and activated the JNK/c-Jun pathway. Both STR and SP600125 suppressed SM-induced JNK phosphorylation and ANP upregulation. SM also increased Piezo1 expression and intracellular calcium. Piezo1 knockdown effectively prevented SM-induced JNK/c-Jun activation, ANP upregulation, and the increase in intracellular calcium, achieving an effect comparable to that of STR and SP600125. Conversely, Yoda1, a Piezo1 activator, mimicked the SM response under normal gravity by activating JNK/c-Jun and increasing ANP expression. STR inhibited SM-induced upregulation of Piezo1 expression. SP600125 blocked JNK/c-Jun activation and ANP upregulation but failed to prevent SM-induced calcium elevation.</div></div><div><h3>Conclusions</h3><div>Short-term simulated weightlessness upregulates ANP expression in atrial cardiomyocytes via activation of the Piezo1 channel. Piezo1 mediates this effect by increasing intracellular calcium and subsequently activating the JNK/c-Jun signaling pathway. These findings identify Piezo1 as a critical upstream mechanosensor for microgravity-induced ANP dysregulation and highlight its potential as a therapeutic target for mitigating associated cardiovascular deconditioning, such as PSOI.</div></div>","PeriodicalId":16920,"journal":{"name":"Journal of Radiation Research and Applied Sciences","volume":"19 1","pages":"Article 102074"},"PeriodicalIF":2.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145683111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SIRT7-mediated desuccinylation of PRMT5 activates the ERK1/2-VEGF-C axis to promote lymphangiogenesis in gastric cancer","authors":"Hongpeng Zhao,&nbsp;Zhihua Liang,&nbsp;Feng Li,&nbsp;Haihua Yu,&nbsp;Kai Li,&nbsp;Bo Liu","doi":"10.1016/j.jrras.2025.102120","DOIUrl":"10.1016/j.jrras.2025.102120","url":null,"abstract":"<div><h3>Background</h3><div>Lymphangiogenesis plays a significant role in tumor metastasis, making it a critical target for cancer therapies. PRMT5, an oncogene involved in various cellular processes, and SIRT7, a desuccinylase, regulate this process in gastric cancer (GC) through complex signaling pathways. However, the specific role of PRMT5 in tumor-induced lymphangiogenesis remains largely unexplored. This study investigates the role of SIRT7 in modulating PRMT5 expression and its impact on lymphangiogenesis through the ERK1/2 signaling pathway in GC.</div></div><div><h3>Methods</h3><div>All functional assays were performed using human lymphatic endothelial cells (HLECs). HLECs were subjected to PRMT5 and SIRT7 knockdown or overexpression via lentiviral transfection. Cell proliferation was evaluated by CCK-8 assay, tube formation was assessed using Matrigel assays, and protein expression was detected by Western blotting. VEGF-C secretion was measured by ELISA, and Co-immunoprecipitation was performed to examine the interaction between SIRT7 and PRMT5. The ERK1/2 signaling pathway was analyzed to elucidate downstream mechanisms.</div></div><div><h3>Results</h3><div>PRMT5 knockdown inhibited lymphangiogenesis and reduced VEGF-C expression, while SIRT7 suppression increased PRMT5 levels, enhancing endothelial cell proliferation and tube formation. Western blotting showed that PRMT5 activates the ERK1/2 pathway, which is essential for VEGF-C regulation. Co-immunoprecipitation further confirmed a direct interaction between SIRT7 and PRMT5, supporting the regulatory link between these proteins.</div></div><div><h3>Conclusions</h3><div>We identify the SIRT7/PRMT5/ERK1/2/VEGF-C axis as a novel and key regulator of lymphangiogenesis in GC, with significant potential therapeutic implications.</div></div>","PeriodicalId":16920,"journal":{"name":"Journal of Radiation Research and Applied Sciences","volume":"19 1","pages":"Article 102120"},"PeriodicalIF":2.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145924667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dosimetric impact of vacuum bag vs. abdominal compression fixation on PTV margins in IMRT for hepatocellular carcinoma with lymph node metastasis","authors":"Zhenghuan Li ,&nbsp;Fantu Kong ,&nbsp;Manya Wu ,&nbsp;Eryan Sun ,&nbsp;Zhi Zhong ,&nbsp;Chao Wang ,&nbsp;Ru Wang ,&nbsp;Yadi He ,&nbsp;Pan Liu ,&nbsp;Jie Dong ,&nbsp;Huamei Yan ,&nbsp;Gaokui He","doi":"10.1016/j.jrras.2026.102166","DOIUrl":"10.1016/j.jrras.2026.102166","url":null,"abstract":"<div><h3>Purpose</h3><div>To compare setup errors between vacuum-bag with thermoplastic shell (VB) and abdominal compression with foam (AC) immobilization techniques in radiotherapy for hepatocellular carcinoma (HCC) with lymph node metastases (LNM), and evaluate their impacts on planning target volume (PTV) margins and dosimetry.</div></div><div><h3>Methods</h3><div>This retrospective cohort study of 34 HCC patients with LNM (VB group: n = 17; AC group: n = 17) was performed. Setup errors were verified by cone-beam CT (CBCT), and PTV margins were calculated using the van Herk formula (MPTV = 2.5Σ+0.7σ). Dosimetric parameters (conformity index, CI; homogeneity index, HI) and organ-at-risk (OAR) doses were compared.</div></div><div><h3>Results</h3><div>The VB group demonstrated significantly smaller calculated PTV margins in the superior-inferior (Y-axis) direction (0.32 cm vs. 0.57 cm, p = 0.001). Systematic errors (Σ) were lower for VB in the X and Y axes, while random errors (σ) were lower in the Z-axis. Dosimetrically, the VB group achieved significantly better dose conformity (CI: 0.24 ± 1.09 % vs. −0.76 ± 1.50 %, p = 0.04). No statistically significant differences were observed in other PTV parameters (Dmax, Dmean, TC, HI) or in the majority of OAR dose parameters, although a trend favoring VB was noted in several instances (e.g., Liver V30, p = 0.04).</div></div><div><h3>Conclusion</h3><div>For HCC patients with LNM undergoing IMRT, vacuum bag fixation significantly reduces PTV margins along the superior-inferior direction and improves dose conformity compared to abdominal compression, offering a potential for optimized treatment planning.</div></div>","PeriodicalId":16920,"journal":{"name":"Journal of Radiation Research and Applied Sciences","volume":"19 1","pages":"Article 102166"},"PeriodicalIF":2.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146034487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predicting cognitive decline in Parkinson's disease using multimodal MRI features and serum inflammatory markers","authors":"Ren Dong ,&nbsp;Haiqing Shen ,&nbsp;Qianning Li","doi":"10.1016/j.jrras.2025.102124","DOIUrl":"10.1016/j.jrras.2025.102124","url":null,"abstract":"<div><h3>Purpose</h3><div>Cognitive decline is a significant concern in Parkinson's disease, affecting patient quality of life and increasing healthcare burden. This study aims to construct and validate a risk prediction model for cognitive decline in Parkinson's disease patients using multimodal MRI features and serum inflammatory markers.</div></div><div><h3>Methods</h3><div>A retrospective case-control study was conducted involving 413 Parkinson's disease patients with normal cognition at baseline. These patients were divided into a primary cohort (n = 321) and an external validation cohort (n = 92). Patients were followed for 1–4 years, and patients with disease progression were compared with those without progression. Multimodal MRI scans and serum inflammatory marker assessments were performed. Univariate, multivariate logistic regression, and receiver operator characteristic (ROC) analyses were used to identify predictors of cognitive decline.</div></div><div><h3>Results</h3><div>Compared with the non-progression group, the progression group showed significant differences in T1 characteristics, including cortical thickness of the left hemisphere (LH) postcentral gyrus (P = 0.008), left hippocampal volume (P = 0.005), and right hippocampal volume (P = 0.004). The progression group showed significant differences in Susceptibility-Weighted Imaging (SWI) characteristics. Symptoms on the opposite side of Substantia Nigra pars compacta (SNc) were more pronounced in the non-progression group compared to the progression group (P = 0.006). Similarly, for symptoms on the same side of SNc, the non-progression group showed more prominent findings than the progression group (P &lt; 0.001). Serum inflammatory marker analysis indicated that the progression group also showed significant differences in lymphocyte count (P = 0.023), neutrophil-to-lymphocyte ratio (NLR) (P = 0.002), lymphocyte-to-monocyte ratio (LMR) (P = 0.003), and albumin-to-fibrinogen ratio (AFR) (P = 0.004). The combined prediction model demonstrated high diagnostic accuracy, with an area under the curve (AUC) of 0.809 in the primary cohort and 0.812 in the external validation cohort.</div></div><div><h3>Conclusion</h3><div>The developed model combining multimodal MRI features and serum inflammatory markers effectively identifies key factors influencing cognitive decline in Parkinson's disease, offering high diagnostic accuracy and valuable clinical insights for early detection and personalized treatment.</div></div>","PeriodicalId":16920,"journal":{"name":"Journal of Radiation Research and Applied Sciences","volume":"19 1","pages":"Article 102124"},"PeriodicalIF":2.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145787194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SF3B3-mediated alternative splicing of ATP2B4 via exon 21 skipping contributes to the progression of bladder cancer","authors":"Cong Cheng ,&nbsp;Tao Yang ,&nbsp;Li Wang,&nbsp;Youji Yan,&nbsp;Yixiang Liao","doi":"10.1016/j.jrras.2025.102121","DOIUrl":"10.1016/j.jrras.2025.102121","url":null,"abstract":"<div><h3>Background</h3><div>While alternative splicing is increasingly recognized in cancer, its specific regulators and functional consequences in bladder cancer remain largely uncharted. This study aims to investigate the role of abnormal alternative splicing of ATP2B4 in the progression of bladder cancer.</div></div><div><h3>Methods</h3><div>Exon sequencing data from The Cancer Genome Atlas were analyzed using bioinformatics algorithms, revealing significant exon skipping events in bladder cancer. Then, confirm the dysregulated expression of specific transcripts in bladder cancer tissues. Additionally, ATP2B4 was overexpressed or knocked down in tumor cells for in vitro functional experiments.</div></div><div><h3>Results</h3><div>Experimental results show that ATP2B4 produces two transcripts: the full-length transcript (ATP2B4-V1) and the spliced transcript (ATP2B4-V2), where the spliced transcript skips exon 21. ATP2B4-v2 is highly expressed in bladder cancer tissues, while ATP2B4-v1 is mainly expressed in non-cancerous tissues and normal bladder epithelium. Moreover, a higher PSI (Percent Spliced In) value for exon 21, indicative of greater ATP2B4-V1 expression, was correlated with improved survival in bladder cancer patients. In vitro functional assays demonstrated that the ATP2B4 splicing variant (ATP2B4-V2) enhanced the proliferation, migration, and invasion of bladder cancer cells, whereas the full-length transcript (ATP2B4-V1) exerted an opposing effect. We also identified a group of splicing factors, among which the splicing factor SF3B3 (Splicing Factor 3b Subunit 3) was found to promote the skipping of exon 21 of ATP2B4, and explored their role in regulating ATP2B4 precursor mRNA. The results showed that SF3B3 could promote the skipping of exon 21 of ATP2B4, resulting in an increased proportion of bladder cancer cells and enhanced carcinogenicity.</div></div><div><h3>Conclusion</h3><div>SF3B3 promotes the skipping of ATP2B4 exon 21, leading to the preferential expression of an oncogenic isoform (ATP2B4-V2) which promotes the malignant phenotype of bladder cancer cells, suggesting that targeting alternative splicing regulation may represent a promising therapeutic strategy.</div></div>","PeriodicalId":16920,"journal":{"name":"Journal of Radiation Research and Applied Sciences","volume":"19 1","pages":"Article 102121"},"PeriodicalIF":2.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145787195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}