Journal of Radiation Research and Applied Sciences最新文献

{"title":"Single-cell transcriptomics identifies keratinocyte-centered lactylation modulation and experimentally validated biomarkers for psoriasis diagnosis","authors":"Yadi Li,&nbsp;Shushu Lv,&nbsp;Yurong Li,&nbsp;Shufang Chai,&nbsp;Zhusheng Yang,&nbsp;Xiumin Yang,&nbsp;Aihua Wei","doi":"10.1016/j.jrras.2026.102591","DOIUrl":"10.1016/j.jrras.2026.102591","url":null,"abstract":"<div><h3>Background</h3><div>Psoriasis (PS) involves lactylation-driven keratinocyte and immune dysfunction. This study sought to identify key lactylation-related biomarkers and networks for improved diagnosis and therapy.</div></div><div><h3>Methods</h3><div>PS-related transcriptome and single-cell RNA sequencing data were obtained from Gene Expression Omnibus (GEO). scRNA-seq analysis identified key cell populations and lactylation-related gene (LRG) activity. Weighted Gene Co-expression Network Analysis (WGCNA) identified LRG-correlated modules, intersected with differentially expressed genes (DEGs) to find candidate biomarkers. Machine learning algorithms were integrated for feature selection and model construction, with Receiver operating characteristic (ROC) analysis and a nomogram for diagnostic evaluation. Regulatory networks and drug prediction were established, followed by molecular docking. Biomarker expression was validated by Quantitative reverse transcription polymerase chain reaction (RT-qPCR) and Western blot in peripheral blood from PS patients and healthy controls.</div></div><div><h3>Results</h3><div>Single-cell RNA sequencing identified keratinocytes as the key PS-associated cell type with highest LRG activity. WGCNA revealed six LRG-related modules (921 genes) enriched in ribonucleoprotein biogenesis and mitochondrial complexes. Intersection with DEGs highlighted 20 hub genes involved in cell cycle pathways. A four-biomarker model (<em>UBE2T</em>, <em>FGFBP1</em>, <em>EIF4EBP1</em>, <em>GINS3</em>) achieved high diagnostic accuracy (Area Under Curve (AUC) &gt; 0.9) and was integrated into nomogram with high clinical utility. RT-qPCR confirmed significant upregulation of all four genes in PS patient samples (<em>P</em> &lt; 0.05), with <em>FGFBP1</em> and <em>GINS3</em> showing particularly pronounced increases (<em>P</em> &lt; 0.001). Western blot analysis further demonstrated elevated EIF4EBP1 protein expression, consistent with transcriptomic findings. Drug prediction and molecular docking identified resveratrol and calcitriol as potential therapeutic agents targeting <em>GINS3</em>. Biomarker expression correlated significantly with altered immune infiltration, notably M0 macrophages and resting mast cells.</div></div><div><h3>Conclusion</h3><div>This study identified <em>UBE2T</em>, <em>FGFBP1</em>, <em>EIF4EBP1</em> and <em>GINS3</em> as key PS biomarkers with high diagnostic accuracy. These biomarkers show significant immune infiltration correlations and provide crucial insights for PS pathogenesis and clinical management.</div></div>","PeriodicalId":16920,"journal":{"name":"Journal of Radiation Research and Applied Sciences","volume":"19 3","pages":"Article 102591"},"PeriodicalIF":3.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148951493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Qufeng Zhijing Capsule attenuates airway inflammation in experimental asthma and is associated with c-Myc/YTHDF1/TRPV1-related signaling","authors":"Yugang Wu,&nbsp;Jie Wen,&nbsp;Juanli Liu,&nbsp;Jianjiang Luo","doi":"10.1016/j.jrras.2026.102607","DOIUrl":"10.1016/j.jrras.2026.102607","url":null,"abstract":"<div><h3>Background</h3><div>Qufeng Zhijing Capsule (QFZJ) is a traditional Chinese medicine formulation used for asthma-related respiratory symptoms, but its effects on airway epithelial inflammation and the underlying molecular mechanisms remain unclear.</div></div><div><h3>Methods</h3><div>OVA-induced asthmatic rats received QFZJ at 0.5, 1.0, or 2.0 g/kg/day. BEAS-2B cells were pretreated with QFZJ-containing serum before LPS stimulation. Airway inflammation, cell viability, apoptosis, intracellular Ca²⁺ responses, molecular expression, c-Myc enrichment at the YTHDF1 promoter, and TRPV1 mRNA m6A enrichment were evaluated.</div></div><div><h3>Results</h3><div>Medium-dose QFZJ produced the most consistent improvement in lung pathology and reduced inflammatory cytokines and epithelial alarmins in OVA-challenged rats. QFZJ also decreased the expression of c-Myc, YTHDF1, TRPV1, c-Fos, and c-Jun. In LPS-stimulated BEAS-2B cells, QFZJ-containing serum improved cell viability, reduced apoptosis and inflammatory mediator release, and attenuated intracellular Ca²⁺ responses. Overexpression of c-Myc or TRPV1 partially diminished these effects. ChIP-qPCR showed enrichment of c-Myc at a predicted region of the YTHDF1 promoter, whereas m6A-RIP-qPCR showed that QFZJ reduced LPS-induced m6A enrichment of TRPV1 mRNA.</div></div><div><h3>Conclusion</h3><div>These findings support the involvement of c-Myc/YTHDF1/TRPV1-related signaling in the protective effects of QFZJ against airway epithelial inflammation. However, further loss-of-function and direct molecular interaction studies are required to establish the causal and hierarchical relationships within this pathway.</div></div>","PeriodicalId":16920,"journal":{"name":"Journal of Radiation Research and Applied Sciences","volume":"19 3","pages":"Article 102607"},"PeriodicalIF":3.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148951495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of incident particle energy on DNA damage formation in a pUC19 plasmid: A Geant4-DNA study","authors":"Samar A. Alghamdi,&nbsp;Naseeba N. Alotaiq,&nbsp;Nusaiba S. Satti,&nbsp;Wasmia M. Dahan,&nbsp;Tarig G. Ibrahim,&nbsp;Omamah H. Alnaseef,&nbsp;Hanan F. Akhdar","doi":"10.1016/j.jrras.2026.102606","DOIUrl":"10.1016/j.jrras.2026.102606","url":null,"abstract":"<div><div>Understanding how radiation quality and particle energy influence DNA damage formation is essential for radiation biology, radiation protection, and particle therapy applications. In this study, the Geant4-DNA track-structure Monte Carlo toolkit (version 11.3.0) was employed to investigate DNA damage formation in a geometrically explicit pUC19 plasmid model following electron and proton irradiation. The simulations incorporated detailed particle transport in liquid water, radiation chemistry, and DNA damage scoring capabilities provided by the Geant4-DNA framework.</div><div>Simulations were performed for monoenergetic electrons (0.1 keV–3 MeV) and protons (0.1–100 MeV). DNA damage was quantified in terms of single-strand breaks (SSBs), double-strand breaks (DSBs), and damage complexity using the built-in Geant4-DNA plasmid damage scorer. To ensure reliable statistics across the investigated energy spectrum, an adaptive sampling strategy ranging from 10⁴ to 10⁷ primary particles was employed depending on particle energy.</div><div>The results demonstrated a pronounced dependence of DNA damage formation on both particle type and incident energy. Electron-induced strand-break yields exhibited a maximum near 1 keV and decreased progressively at higher energies. In contrast, proton irradiation produced substantially larger DNA damage yields, with maximum strand-break and DSB production occurring at 0.1 MeV. The highest proton-induced DSB yield exceeded the maximum electron induced DSB yield by more than an order of magnitude. Proton irradiation also generated considerably higher levels of complex DNA damage and larger DSB-to-strand-break ratios, indicating enhanced clustering efficiency under dense ionization conditions.</div></div>","PeriodicalId":16920,"journal":{"name":"Journal of Radiation Research and Applied Sciences","volume":"19 3","pages":"Article 102606"},"PeriodicalIF":3.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148951496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fresh bone autograft particles promote H-type vessel-osteogenesis coupling via mechanisms involving SLIT3 and HIF-1α signaling","authors":"Ming Liu,&nbsp;Xiaohu Ma,&nbsp;Xiaojin Wu,&nbsp;Jinguo Ma,&nbsp;Binghan Chen,&nbsp;Yanbin Tian","doi":"10.1016/j.jrras.2026.102579","DOIUrl":"10.1016/j.jrras.2026.102579","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to investigate the molecular mechanisms by which fresh bone autograft particles (FBAP) promotes bone repair and regeneration through mechanisms involving Slit Guidance Ligand 3 (SLIT3) and HIF-1α signaling, which regulating the coupling of H-type vessels and osteogenesis.</div></div><div><h3>Methods</h3><div>hFOB1.19 osteoblasts and human bone marrow-derived endothelial progenitor cells (EPCs) were treated with varying concentrations of FBAP in vitro. Cell viability was assessed using the CCK-8 assay. Osteogenic capacity was evaluated by alkaline phosphatase (ALP) staining and alizarin red staining. Protein expression was detected by immunofluorescence and Western blot. Cell migration was assessed using Transwell assays, and angiogenesis was evaluated by in vitro tube formation assays. Additionally, <em>SLIT3</em> was knocked down using shRNA, and the HIF-1α inhibitor YC-1 was used to explore their effects on the actions of FBAP.</div></div><div><h3>Results</h3><div>FBAP significantly enhanced the viability, ALP activity, and mineralization of hFOB1.19 cells in a dose-dependent manner. Alizarin red staining and Western blot analysis showed that FBAP promoted the expression of osteogenic proteins (ALP, OCN, and COL1A1). In EPCs, FBAP increased the expression of CD31 and Emcn, enhanced cell migration and tube formation abilities, and activated the SLIT3/ROBO1 and HIF-1α signaling pathways. Knockdown of <em>SLIT3</em> and treatment with YC-1 inhibited the pro-osteogenic and pro-angiogenic effects of FBAP.</div></div><div><h3>Conclusion</h3><div>These findings indicate that FBAP promotes bone repair and regeneration, with SLIT3 and HIF-1α playing essential roles in regulating the coupling of H-type vessels and osteogenesis.</div></div>","PeriodicalId":16920,"journal":{"name":"Journal of Radiation Research and Applied Sciences","volume":"19 3","pages":"Article 102579"},"PeriodicalIF":3.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148951494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Occupational radiation exposure among healthcare workers in fluoroscopy-guided interventional procedures: A multi-centre longitudinal study in Tanzania","authors":"Jackline Yonahema Yonaeli,&nbsp;Justine E. Ngaile,&nbsp;Nelson Mpumi,&nbsp;Dennis Amos Mwalongo,&nbsp;Siwidhani Thomas Ndovi,&nbsp;Nils Haneklaus","doi":"10.1016/j.jrras.2026.102604","DOIUrl":"10.1016/j.jrras.2026.102604","url":null,"abstract":"<div><div>Fluoroscopy-guided interventional procedures (FGIPs) are increasingly used in modern medicine because they offer minimally invasive diagnostic and therapeutic options, but they also expose healthcare workers to ionizing radiation. In Tanzania, occupational dose data for staff involved in FGIPs remain limited. This study assessed occupational radiation exposure among workers involved in FGIPs in selected Tanzanian referral hospitals, with emphasis on differences across professional categories, hospitals, and monitoring periods. A hybrid retrospective-prospective longitudinal study was conducted among 37 occupationally exposed workers from four referral hospitals. Retrospective dose records were collected for two three-month quarters from May to October 2025, followed by prospective monitoring for two additional quarters from November 2025 to April 2026 using thermoluminescent dosimeters (LiF:Mg,Ti). The mean annual personal dose equivalent was 1.14 ± 0.20 mSv (range: 0.60–1.39 mSv). All values were below the ICRP-recommended occupational dose limit of 20 mSv year⁻¹. Mean annual personal dose equivalent differed significantly across professional categories (Welch ANOVA, p &lt; 0.001; η² = 0.658), whereas differences between hospitals were not significant (ANOVA, p = 0.318; η² = 0.10). Linear mixed-effects modelling showed a modest but statistically significant variation in quarterly personal dose equivalent over time (p = 0.029). These findings support continued dose monitoring and targeted radiation protection for personnel with higher occupational exposure.</div></div>","PeriodicalId":16920,"journal":{"name":"Journal of Radiation Research and Applied Sciences","volume":"19 3","pages":"Article 102604"},"PeriodicalIF":3.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148951607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of radiation dose parameters during exposure in cerebral computed tomography angiography","authors":"Nouf Abuhadi","doi":"10.1016/j.jrras.2026.102420","DOIUrl":"10.1016/j.jrras.2026.102420","url":null,"abstract":"<div><div>This study aimed to evaluate the radiation doses for patients during cerebral angiographic computed tomography (CTA) scans using two multidetector CT scanners (MDCTs), to reduce these doses, and to determine institutional diagnostic reference levels (iDRLs). A total of 244 patients were included in the study; 131 (53.7%) were examined using the routine protocol, whereas 113 (46.3%) underwent the dose reduction protocol. All examinations were carried out using two 64-slice MDCT scanners at the radiology department of a Southern-Region of Saudi Arabia hospital: a GE Healthcare, and Siemens scanners. Dose length product (DLP, mGy.cm) and mean volume CT dose index (CTDI, mGy) were used as the dose descriptors according to the International Commission on Radiological Protection (ICRP) recommendations. Data were analyzed using IBM SPSS Statistics (version 26). The overall iDRLs for CTDI_vol and DLP were 64.9 mGy and 2184.4 mGy cm, respectively. These iDRLs, particularly for the DLP, were higher than the reference values for individual CT scans in Saudi Arabia (1026 mGy.cm) as proposed by the Saudi Food and Drug Administration (SFDA), which potentially indicated that these patients had undergone multiple or complex procedures. Notably, the overall radiation burdens were considerably influenced by variables other than age, gender, or duration of exposure. To maintain the minimum dose requirements without compromising diagnostic accuracy, these findings highlight the importance of focusing on the cumulative DLP, particularly for repeated or prolonged scans. The findings also stress the need for the regular review and updating of imaging methods and provide vital information for improving CT procedures and enhancing patient safety by using more effective dose reduction techniques.</div></div>","PeriodicalId":16920,"journal":{"name":"Journal of Radiation Research and Applied Sciences","volume":"19 2","pages":"Article 102420"},"PeriodicalIF":2.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148230848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hewei Zhiou Recipe relieves CINV through the 5-HT3R/SERT signaling pathway","authors":"Jing Zhang ,&nbsp;Jingyu Feng ,&nbsp;Yang Li ,&nbsp;Panyan Mo ,&nbsp;Chao Zeng ,&nbsp;Lizhu Lin","doi":"10.1016/j.jrras.2026.102400","DOIUrl":"10.1016/j.jrras.2026.102400","url":null,"abstract":"<div><h3>Objectives</h3><div>Investigating the antiemetic effect of HWZO and the underlying molecular mechanism of its antiemetic effect.</div></div><div><h3>Methods</h3><div>CINV models of rats and cells were constructed using cisplatin and substance P, respectively. The intake of kaolin observed the antiemetic effect of HWZO. Effective cell concentrations of HWZO were screened, and 5-HT3R knockdown and overexpression cell lines were constructed. The expression levels of essential markers related to 5-HT and substance P in serum, ileum, and medulla oblongata of rats, as well as cell models, were determined by ELISA, RT-qPCR, and WB.</div></div><div><h3>Results</h3><div>Results of animal experiments showed that HWZO significantly reduced kaolin intake, increased food-intake and body weight in rats, and simultaneously suppressed the expression of key emetogenic molecular markers in the peripheral and central nervous system (<em>P</em> &lt; 0.05). In cellular experiments, screening for effective concentrations of HWZO was first performed, and the results showed that high doses had the best effect (<em>P</em> &lt; 0.05). HWZO treatment decreased 5-HT, SP, and SPR levels while increasing SERT expression in cellular models. Subsequently, 5-HT3R knockdown and overexpression cell lines were constructed, and the expression levels of 5-HT, SP, and other related molecular markers of vomiting after HWZO administration were significantly increased in 5-HT3R overexpression cells (<em>P</em> &lt; 0.05), while decreased in 5-HT3R knockdown cells (<em>P</em> &lt; 0.05).</div></div><div><h3>Conclusion</h3><div>HWZO is expected to be an effective and safe adjuvant therapy for the treatment of CINV through a multi-targeted and multi-pathway mechanism of action, especially by modulating the 5-HT3R/SERT and SP/SPR signaling pathways.</div></div>","PeriodicalId":16920,"journal":{"name":"Journal of Radiation Research and Applied Sciences","volume":"19 2","pages":"Article 102400"},"PeriodicalIF":2.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148230844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular mechanism of compound anisodine in improving ischemic ophthalmopathy by regulating AMPK/mTOR pathway","authors":"Weijie Yang ,&nbsp;Jing Chen ,&nbsp;Zhengrong Shen ,&nbsp;Hong Xu","doi":"10.1016/j.jrras.2026.102397","DOIUrl":"10.1016/j.jrras.2026.102397","url":null,"abstract":"<div><h3>Objective</h3><div>This work aimed to explore the molecular mechanism by which Compound anisodine (CA) improved ischemic ophthalmopathy (IOP) in rats through regulation of the AMP-activated protein kinase (AMPK)/mechanistic target of rapamycin (mTOR) pathway.</div></div><div><h3>Methods</h3><div>Sixty Sprague Dawley rats were assigned into a blank control (BC) group (no treatment), an IOP group (IOP model established), normal saline (NS) group (additional injection sodium chloride based on IOP group), CA group (additional CA injection based on IOP group), CA + AMPKi group (additional AMPK inhibitor Compound C based on CA group), and CA + AMPKa group (additional AMPK activator Acadesine based CA group). Changes in retinal structure and function were assessed using iris imaging by scanning electron microscopy (SEM), retinal examination, blood oxygenation monitoring (BOM) parameters (ocular blood oxygen saturation (BOS) and perfusion index (PI)) in the eyes. Retinal tissues were acquired for hematoxylin-eosin (H&amp;E) staining to observe pathological structural changes, and western blotting was carried out to detect AMPK and mTOR protein expressions.</div></div><div><h3>Results</h3><div>Relative to BC group, IOP and NS groups showed reduced iris vascular area and pericyte count, decreased rod cell response, maximum mixed response, and oscillatory potentials (OPs), lowered ocular blood oxygen saturation (BOS) and perfusion index (PI), and reduced retinal, inner nuclear layer (INL), and outer nuclear layer (ONL) thicknesses. Additionally, it was observed that retinal LC3 II/I and p-mTOR expressions were downregulated, while p-AMPK expression was upregulated (<em>P</em> &lt; 0.05). In contrast to the IOP group, the CA group exhibited substantial improvement in all the above indicators (<em>P</em> &lt; 0.05). In addition, the therapeutic effects of CA were reversed in the CA + AMPKi group, while the therapeutic outcomes of CA were further enhanced in the CA + AMPKa group.</div></div><div><h3>Conclusion</h3><div>CA could mediate the AMPK/mTOR pathway to improve retinal damage in rats with IOP, providing a new molecular basis for preventing and treating IOP.</div></div>","PeriodicalId":16920,"journal":{"name":"Journal of Radiation Research and Applied Sciences","volume":"19 2","pages":"Article 102397"},"PeriodicalIF":2.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148230956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic renoprotection and renal targeting of radiolabeled finerenone combined with compound α-Ketoacid tablets in type 2 diabetic nephropathy","authors":"Zhenyan Wang,&nbsp;Xuefen Li,&nbsp;Xiaoman Zheng,&nbsp;Jiachang Fu,&nbsp;Weiting Wang,&nbsp;Li Li","doi":"10.1016/j.jrras.2026.102339","DOIUrl":"10.1016/j.jrras.2026.102339","url":null,"abstract":"<div><h3>Background and objectives</h3><div>Effective therapies to halt diabetic nephropathy (DN) progression are needed. Finerenone mitigates renal inflammation and fibrosis, while Compound α-Ketoacid Tablets reduce renal metabolic load. This study evaluated the synergistic renoprotective effect of their combination and assessed renal drug targeting using radionuclide imaging in type 2 DN.</div></div><div><h3>Methods</h3><div>In this study with a hybrid design (n = 220), patients received Finerenone monotherapy or combination therapy with Compound α-Ketoacid Tablets for 3 months. All participants prospectively underwent dynamic renal SPECT/CT with ⁹⁹ᵐTc-Finerenone as per a pre-specified protocol. Metabolic parameters, urinary biomarkers, renal function, and safety were assessed. Renal cortical tracer uptake and retention were quantified. Statistical analyses included sample size calculation, handling of missing data, and correction for multiple comparisons.</div></div><div><h3>Results</h3><div>The combination therapy group demonstrated superior improvements in lipid profiles, a significantly higher overall response rate in urinary albumin-to-creatinine ratio (UACR) (68.3% vs. 25.0%, P &lt; 0.001), and better preservation of estimated glomerular filtration rate (eGFR) compared to the monotherapy group (all P &lt; 0.05). Modest but statistically significant improvements in glycemic control were also observed. SPECT/CT revealed 32.7% higher renal cortical uptake and prolonged tracer retention in the combination group, which strongly correlated with the improvements in UACR and eGFR (r = 0.82, P &lt; 0.01). Both regimens were well-tolerated with a similar safety profile.</div></div><div><h3>Conclusion</h3><div>The combination of Finerenone and Compound α-Ketoacid Tablets confers synergistic renoprotection in type 2 DN. The enhanced renal targeting and prolonged retention of radiolabeled Finerenone suggest modulated intrarenal drug distribution. This approach highlights the utility of nuclear imaging in visualizing and evaluating targeted therapies for DN.</div></div>","PeriodicalId":16920,"journal":{"name":"Journal of Radiation Research and Applied Sciences","volume":"19 2","pages":"Article 102339"},"PeriodicalIF":2.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148230955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three-dimensional morphological study of craniofacial bones in non-syndromic cleft lip and palate patients based on cone-beam computed tomography imaging","authors":"Qiangwei Zhu ,&nbsp;Di Liu ,&nbsp;Jiaocui Wang ,&nbsp;Dandan Li ,&nbsp;Yongchu Pan","doi":"10.1016/j.jrras.2026.102396","DOIUrl":"10.1016/j.jrras.2026.102396","url":null,"abstract":"<div><h3>Background</h3><div>Non-syndromic cleft lip with or without cleft palate (NSCL/P), including unilateral (UCLP) and bilateral (BCLP) types, is a common congenital deformity that affects maxillofacial appearance and function. In order to describe the postoperative craniofacial morphology in UCLP and BCLP patients after prior surgical intervention, this study used cone-beam computed tomography (CBCT) to quantify primary maxillofacial characteristics in UCLP and BCLP patients and to assess the secondary post-surgical changes in the maxillary bones.</div></div><div><h3>Methods</h3><div>This retrospective case-control study included 111 adolescents treated between January 2018 and December 2022. Participants were divided into three groups: UCLP (n = 37), BCLP (n = 37), and orthodontic control (n = 37). CBCT scans were obtained, and 3D reconstruction was performed using Mimics 21.0 software to establish a standardized coordinate system. Measurements of maxillary width, height, depth, and mandibular length were compared across groups using SPSS 26.0.</div></div><div><h3>Results</h3><div>In the UCLP group, maxillary width on the cleft side was significantly greater than in the control group, deviating from the midline (<em>P</em> &lt; 0.05). The BCLP group showed a bilateral increase in maxillary width, with a greater distance from the cleft side maxilla to the sagittal plane (<em>P</em> &lt; 0.05). Both cleft groups had significantly reduced maxillary height compared to controls (<em>P</em> &lt; 0.05), indicating vertical underdevelopment. In the BCLP group, maxillary asymmetry was more pronounced, with inward displacement of key landmarks. Compared to the control, the cleft group showed no significant difference in mandibular length (<em>P</em> &gt; 0.05), indicating it was unaffected by the cleft.</div></div><div><h3>Conclusion</h3><div>CBCT provides precise 3D measurements that reveal complex maxillary abnormalities in UCLP and BCLP patients, including width anomalies, vertical underdevelopment, and sagittal retrusion. The mandible remains unaffected in length, highlighting that the primary deformity lies in maxillary morphology rather than size. This study identifies distinct skeletal features for different CLP types, offering a foundation for individualized treatment planning and efficacy assessment.</div></div>","PeriodicalId":16920,"journal":{"name":"Journal of Radiation Research and Applied Sciences","volume":"19 2","pages":"Article 102396"},"PeriodicalIF":2.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148230845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}