Journal of Physiological Sciences最新文献_第6页

The evidence to date: implications of l-ascorbic acid in the pathophysiology of aging. 迄今为止的证据：抗坏血酸对衰老病理生理学的影响。

IF 2.6 4区医学

Journal of Physiological Sciences Pub Date : 2024-05-11 DOI: 10.1186/s12576-024-00922-7

Ayami Sato, Yoshitaka Kondo, Akihito Ishigami

引用次数: 0

Negative regulation of thyroid adenoma-associated protein (THADA) in the cardiac glycoside-induced anti-cancer effect. 甲状腺腺瘤相关蛋白（THADA）在强心甙诱导的抗癌效应中的负调控作用

IF 2.6 4区医学

Journal of Physiological Sciences Pub Date : 2024-04-01 DOI: 10.1186/s12576-024-00914-7

Mizuki Katoh, Takuto Fujii, Yoshiaki Tabuchi, Takahiro Shimizu, Hideki Sakai

{"title":"Negative regulation of thyroid adenoma-associated protein (THADA) in the cardiac glycoside-induced anti-cancer effect.","authors":"Mizuki Katoh, Takuto Fujii, Yoshiaki Tabuchi, Takahiro Shimizu, Hideki Sakai","doi":"10.1186/s12576-024-00914-7","DOIUrl":"10.1186/s12576-024-00914-7","url":null,"abstract":"Cardiac glycosides, known as inhibitors of Na+,K+-ATPase, have anti-cancer effects such as suppression of cancer cell proliferation and induction of cancer cell death. Here, we examined the signaling pathway elicited by cardiac glycosides in the human hepatocellular carcinoma HepG2 cells and human epidermoid carcinoma KB cells. Three kinds of cardiac glycosides (ouabain, oleandrin, and digoxin) inhibited the cancer cell proliferation and decreased the expression level of thyroid adenoma-associated protein (THADA). Interestingly, the knockdown of THADA inhibited cancer cell proliferation, and the proliferation was significantly rescued by re-expression of THADA in the THADA-knockdown cells. In addition, the THADA-knockdown markedly decreased the expression level of L-type amino acid transporter LAT1. Cardiac glycosides also reduced the LAT1 expression. The LAT1 inhibitor, JPH203, significantly weakened the cancer cell proliferation. These results suggest that the binding of cardiac glycosides to Na+,K+-ATPase negatively regulates the THADA-LAT1 pathway, exerting the anti-proliferative effect in cancer cells.","PeriodicalId":16832,"journal":{"name":"Journal of Physiological Sciences","volume":"74 1","pages":"23"},"PeriodicalIF":2.6,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10985892/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140336085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acetate ameliorates ovarian mitochondrial dysfunction in letrozole-induced polycystic ovarian syndrome rat model by improving mitofusin-2. 醋酸酯通过改善丝裂霉素-2，改善来曲唑诱导的多囊卵巢综合征大鼠模型的卵巢线粒体功能障碍。

IF 2.6 4区医学

Journal of Physiological Sciences Pub Date : 2024-04-01 DOI: 10.1186/s12576-024-00908-5

Kehinde S Olaniyi, Stephanie E Areloegbe

{"title":"Acetate ameliorates ovarian mitochondrial dysfunction in letrozole-induced polycystic ovarian syndrome rat model by improving mitofusin-2.","authors":"Kehinde S Olaniyi, Stephanie E Areloegbe","doi":"10.1186/s12576-024-00908-5","DOIUrl":"10.1186/s12576-024-00908-5","url":null,"abstract":"Androgen excess and metabolic abnormality largely contribute to the pathogenesis of polycystic ovarian syndrome (PCOS), which primarily precipitates ovarian dysfunction and infertility in reproductive-age women. Impaired mitochondrial function and epigenetic alteration have been linked to the development of PCOS. However, it is unknown whether acetate would exert a therapeutic effect on ovarian mitochondrial dysfunction in PCOS. Herein, the study hypothesized that acetate reverses ovarian mitochondrial dysfunction in experimental PCOS rat model, possibly through modulation of mitofusin-2 (MFn2). Eight-week-old female Wistar rats were randomized into four groups (n = 5). Induction of PCOS was performed by 1 mg/kg letrozole (p.o.), administered for 21 days. Thereafter, the rats were treated with acetate (200 mg/kg; p.o.) for 6 weeks. The PCOS rats demonstrated androgen excess, multiple ovarian cysts, elevated anti-mullerian hormone and leptin and decreased SHBG, adiponectin and 17-β estradiol with corresponding increase in ovarian transforming growth factor-β1. Additionally, inflammation (tumor growth factor and nuclear factor-kB), elevated caspase-6, decreased hypoxia-inducible factor-1α and elevated histone deacetylase-2 (HDAC2) were observed in the ovaries of PCOS rats, while mitochondrial abnormality with evidence of decreased adenosine triphosphate synthase and MFn2 was observed in rats with PCOS. Treatment with acetate reversed the alterations. The present results collectively suggest that acetate ameliorates ovarian mitochondrial abnormality, a beneficial effect that is accompanied by MFn2 with consequent normalization of reproductive-endocrine profile and ovarian function. Perhaps, the present data provide hope for PCOS individuals that suffer infertility.","PeriodicalId":16832,"journal":{"name":"Journal of Physiological Sciences","volume":"74 1","pages":"22"},"PeriodicalIF":2.6,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10983676/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140336084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An interactive simulator to deepen the understanding of Guyton's venous return curve. 互动模拟器加深了对盖顿静脉回流曲线的理解。

IF 2.6 4区医学

Journal of Physiological Sciences Pub Date : 2024-03-30 DOI: 10.1186/s12576-024-00912-9

Noritaka Mamorita, Akihiro Takeuchi, Hirotoshi Kamata

{"title":"An interactive simulator to deepen the understanding of Guyton's venous return curve.","authors":"Noritaka Mamorita, Akihiro Takeuchi, Hirotoshi Kamata","doi":"10.1186/s12576-024-00912-9","DOIUrl":"10.1186/s12576-024-00912-9","url":null,"abstract":"Mean circulatory filling pressure, venous return curve, and Guyton's graphical analysis are basic concepts in cardiovascular physiology. However, some medical students may not know how to view and interpret or understand them adequately. To deepen students' understanding of the graphical analysis, in place of having to perform live animal experiments, we developed an interactive cardiovascular simulator, as a self-learning tool, as a web application. The minimum closed-loop model consisted of a ventricle, an artery, resistance, and a vein, excluding venous resistance. The simulator consists of three modules: setting (parameters and simulation modes), calculation, and presentation. In the setting module, the user can interactively customize model parameters, compliances, resistance, Emax of the ventricular contractility, total blood volume, and unstressed volume. The hemodynamics are calculated in three phases: filling (late diastole), ejection (systole), and flow (early diastole). In response to the user's settings, the simulator graphically presents the hemodynamics: the pressure-volume relations of the artery, vein, and ventricle, the venous return curves, and the stroke volume curves. The mean filling pressure is calculated at approximately 7 mmHg at the initial setting. The venous return curves, linear and concave, are dependent on the venous compliance. The hemodynamic equilibrium point is marked on the crossing point of venous return curve and the stroke volume curve. Users can interactively do discovery learning, and try and confirm their interests and get their questions answered about hemodynamic concepts by using the simulator.","PeriodicalId":16832,"journal":{"name":"Journal of Physiological Sciences","volume":"74 1","pages":"21"},"PeriodicalIF":2.6,"publicationDate":"2024-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10981291/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140329901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Regulation of myocardial contraction as revealed by intracellular Ca²⁺ measurements using aequorin. 更正：使用 aequorin 测量细胞内 Ca2+ 揭示的心肌收缩调节。

IF 2.6 4区医学

Journal of Physiological Sciences Pub Date : 2024-03-26 DOI: 10.1186/s12576-024-00915-6

Satoshi Kurihara, Norio Fukuda

引用次数: 0

Influence of age on nicotinic cholinergic regulation of blood flow in rat's olfactory bulb and neocortex. 年龄对大鼠嗅球和新皮质中烟碱胆碱能调节血流的影响

IF 2.6 4区医学

Journal of Physiological Sciences Pub Date : 2024-03-15 DOI: 10.1186/s12576-024-00913-8

Sae Uchida, Fusako Kagitani

{"title":"Influence of age on nicotinic cholinergic regulation of blood flow in rat's olfactory bulb and neocortex.","authors":"Sae Uchida, Fusako Kagitani","doi":"10.1186/s12576-024-00913-8","DOIUrl":"10.1186/s12576-024-00913-8","url":null,"abstract":"The olfactory bulb receives cholinergic basal forebrain inputs as does the neocortex. With a focus on nicotinic acetylcholine receptors (nAChRs), this review article provides an overview and discussion of the following findings: (1) the nAChRs-mediated regulation of regional blood flow in the neocortex and olfactory bulb, (2) the nAChR subtypes that mediate their responses, and (3) their activity in old rats. The activation of the α4β2-like subtype of nAChRs produces vasodilation in the neocortex, and potentiates olfactory bulb vasodilation induced by olfactory stimulation. The nAChR activity producing neocortical vasodilation was similarly maintained in 2-year-old rats as in adult rats, but was clearly reduced in 3-year-old rats. In contrast, nAChR activity in the olfactory bulb was reduced already in 2-year-old rats. Thus, age-related impairment of α4β2-like nAChR function may occur earlier in the olfactory bulb than in the neocortex. Given the findings, the vasodilation induced by α4β2-like nAChR activation may be beneficial for neuroprotection in the neocortex and the olfactory bulb.","PeriodicalId":16832,"journal":{"name":"Journal of Physiological Sciences","volume":"74 1","pages":"18"},"PeriodicalIF":2.6,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10941616/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140140411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Action of GABA_B receptor on local network oscillation in somatosensory cortex of oral part: focusing on NMDA receptor. GABAB 受体对口腔部位躯体感觉皮层局部网络振荡的作用：聚焦 NMDA 受体。

IF 2.6 4区医学

Journal of Physiological Sciences Pub Date : 2024-03-12 DOI: 10.1186/s12576-024-00911-w

Hiroyuki Kanayama, Takashi Tominaga, Yoko Tominaga, Nobuo Kato, Hiroshi Yoshimura

{"title":"Action of GABAB receptor on local network oscillation in somatosensory cortex of oral part: focusing on NMDA receptor.","authors":"Hiroyuki Kanayama, Takashi Tominaga, Yoko Tominaga, Nobuo Kato, Hiroshi Yoshimura","doi":"10.1186/s12576-024-00911-w","DOIUrl":"10.1186/s12576-024-00911-w","url":null,"abstract":"The balance of activity between glutamatergic and GABAergic networks is particularly important for oscillatory neural activities in the brain. Here, we investigated the roles of GABAB receptors in network oscillation in the oral somatosensory cortex (OSC), focusing on NMDA receptors. Neural oscillation at the frequency of 8-10 Hz was elicited in rat brain slices after caffeine application. Oscillations comprised a non-NMDA receptor-dependent initial phase and a later NMDA receptor-dependent oscillatory phase, with the oscillator located in the upper layer of the OSC. Baclofen was applied to investigate the actions of GABAB receptors. The later NMDA receptor-dependent oscillatory phase completely disappeared, but the initial phase did not. These results suggest that GABAB receptors mainly act on NMDA receptor, in which metabotropic actions of GABAB receptors may contribute to the attenuation of NMDA receptor activities. A regulatory system for network oscillation involving GABAB receptors may be present in the OSC.","PeriodicalId":16832,"journal":{"name":"Journal of Physiological Sciences","volume":"74 1","pages":"16"},"PeriodicalIF":2.6,"publicationDate":"2024-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10935845/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140110490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alteration of serotonin release response in the central nucleus of the amygdala to noxious and non-noxious mechanical stimulation in a neuropathic pain model rat. 神经性疼痛模型大鼠杏仁核中枢核对有毒和无毒机械刺激的血清素释放反应的改变

IF 2.6 4区医学

Journal of Physiological Sciences Pub Date : 2024-03-12 DOI: 10.1186/s12576-024-00910-x

Ryota Tokunaga, Hideshi Shibata, Mieko Kurosawa

引用次数: 0

Mechanisms of the TGF-β1/Smad3-signaling pathway in gender differences in alcoholic liver fibrosis. TGF-β1/Smad3信号通路在酒精性肝纤维化性别差异中的作用机制

IF 2.6 4区医学

Journal of Physiological Sciences Pub Date : 2024-02-26 DOI: 10.1186/s12576-024-00901-y

Xiaomin Hong, Sanqiang Li, Renli Luo, Mengli Yang, Junfei Wu, Shuning Chen, Siyu Zhu

{"title":"Mechanisms of the TGF-β1/Smad3-signaling pathway in gender differences in alcoholic liver fibrosis.","authors":"Xiaomin Hong, Sanqiang Li, Renli Luo, Mengli Yang, Junfei Wu, Shuning Chen, Siyu Zhu","doi":"10.1186/s12576-024-00901-y","DOIUrl":"10.1186/s12576-024-00901-y","url":null,"abstract":"The TGF-β1/Smad3-signaling pathway and gender differences were investigated in alcoholic liver fibrosis. Mice were divided into female normal, female model, male normal, and male model groups. Liver injury and fibrosis were assessed using histopathology and serology. Western blotting was performed to analyze the expression of relevant factors. HSC-T6 cells were divided into estradiol + saline, estradiol + ethanol, testosterone + saline, and testosterone + ethanol groups, and similar assessments were conducted in vitro. Compared with the female model group, the male model group exhibited significantly increased GPT, GOT, TNF-α, IL-6, and testosterone levels, fibrosis rate, and TGF-β1, Smad3, and PCNA expression, and significantly decreased estradiol levels and Caspase-3 expression. The apoptosis rate was higher in the estradiol + ethanol group than in the testosterone + ethanol group, although the testosterone + ethanol group exhibited significantly increased TNF-α, IL-6, Collagen-I, α-SMA, TGF-β1, Smad3, and PCNA expression, and significantly decreased Caspase-3 expression. Alcoholic liver fibrosis showed significant gender differences associated with the TGF-β1/Smad3-signaling pathway.","PeriodicalId":16832,"journal":{"name":"Journal of Physiological Sciences","volume":"74 1","pages":"13"},"PeriodicalIF":2.6,"publicationDate":"2024-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10898046/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139972269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Strength training improves heart function, collagen and strength in rats with heart failure. 力量训练可改善心力衰竭大鼠的心脏功能、胶原蛋白和力量。

IF 2.6 4区医学

Journal of Physiological Sciences Pub Date : 2024-02-16 DOI: 10.1186/s12576-024-00899-3

Leisiane G Dias, Carlos H O Reis, Leonardo Dos Santos, Walter Krause Neto, Ana Paula Lima-Leopoldo, Julien S Baker, André S Leopoldo, Danilo S Bocalini

{"title":"Strength training improves heart function, collagen and strength in rats with heart failure.","authors":"Leisiane G Dias, Carlos H O Reis, Leonardo Dos Santos, Walter Krause Neto, Ana Paula Lima-Leopoldo, Julien S Baker, André S Leopoldo, Danilo S Bocalini","doi":"10.1186/s12576-024-00899-3","DOIUrl":"10.1186/s12576-024-00899-3","url":null,"abstract":"Background/objectives: Myocardial infarction (MI) frequently leads to cardiac remodeling and failure with impaired life quality, playing an important role in cardiovascular deaths. Although physical exercise is a well-recognized effective non-pharmacological therapy for cardiovascular diseases, the effects of strength training (ST) on the structural and functional aspects of cardiac remodeling need to be further documented. In this study, we aimed to investigate the role of a linear block ST protocol in the rat model of MI.Methods and results: After 6 weeks of MI induction or sham surgery, male adult rats performed ST for the following 12 weeks. The ladder-based ST program was organized in three mesocycles of 4 weeks, with one load increment for each block according to the maximal carrying load test. After 12 weeks, the infarcted-trained rats exhibited an increase in performance, associated with reduced cardiac hypertrophy and pulmonary congestion compared with the untrained group. Despite not changing MI size, the ST program partially prevented cardiac dilatation and ventricular dysfunction assessed by echocardiography and hemodynamics, and interstitial fibrosis evaluated by histology. In addition, isolated cardiac muscles from infarcted-trained rats had improved contractility parameters in a steady state, and in response to calcium or stimuli pauses.Conclusions: The ST in infarcted rats increased the capacity to carry mass, associated with attenuation of cardiac remodeling and pulmonary congestion with improving cardiac function that could be attributed, at least in part, to the improvement of myocardial contractility.","PeriodicalId":16832,"journal":{"name":"Journal of Physiological Sciences","volume":"74 1","pages":"10"},"PeriodicalIF":2.6,"publicationDate":"2024-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10873996/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139746813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0