Journal of Phycology最新文献_第3页

Functional traits vary with life stage not community context of the tropical secondary foundation macroalga Turbinaria ornata. 热带次生基础大藻的功能性状随其生命阶段而非群落环境而变化。

IF 4.3 3区生物学

Journal of Phycology Pub Date : 2026-10-01 Epub Date: 2026-07-26 DOI: 10.1111/jpy.70213

Katherine Hannibal, Peggy Fong

{"title":"Functional traits vary with life stage not community context of the tropical secondary foundation macroalga Turbinaria ornata.","authors":"Katherine Hannibal, Peggy Fong","doi":"10.1111/jpy.70213","DOIUrl":"10.1111/jpy.70213","url":null,"abstract":"The global decline of coral reefs has led to the emergence of novel communities, including macroalgal-dominated states, that can be both prevalent and persistent, making understanding the dynamics of these emergent communities vital. In the South Pacific, emergent stands of Turbinaria ornata, a secondary foundation species, create an associational refuge that protects understory algae from herbivory. Our objective was to utilize a trait-based framework to examine intraspecific trait variation of T. ornata individuals and to explore if this variation differs across life stage and with position within a canopy-forming stand. We compared four functional traits related to the ecological strategies of resistance to herbivory and physical disturbance of large and recruit T. ornata thalli. We collected individuals in two community contexts-both within and at the edge of stands (n = 22 per treatment, N = 88). Trait distributions varied significantly with life stage but not with community context. Small recruits were less tough, less strong, and less compact than larger canopy-forming thalli, indicating higher vulnerability to herbivory and physical disturbance. Our findings demonstrate that the associational refuge provided by large, canopy-forming individuals is likely crucial for the protection of recruits and, therefore, the persistence of the stand. This refuge may ultimately contribute to the expansion and stability of this emerging secondary foundation species.","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":" ","pages":"1702-1709"},"PeriodicalIF":4.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148592315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Linking variation in genome size to cyst size in chrysophytes. 菊花基因组大小变异与包囊大小的关联。

IF 4.3 3区生物学

Journal of Phycology Pub Date : 2026-10-01 Epub Date: 2026-08-07 DOI: 10.1111/jpy.70217

Natálie Protić, Iva Jadrná, Pavel Škaloud, Petr Knotek, Dora Čertnerová

{"title":"Linking variation in genome size to cyst size in chrysophytes.","authors":"Natálie Protić, Iva Jadrná, Pavel Škaloud, Petr Knotek, Dora Čertnerová","doi":"10.1111/jpy.70217","DOIUrl":"10.1111/jpy.70217","url":null,"abstract":"Genome size varies tremendously across eukaryotes, which often contain far more DNA than expected from their biological complexity. To explain this paradox, selection-based hypotheses propose that genome size evolves through selection acting on life-history traits correlated with the phenotypic effects, independent of its genic content. To test the association between genome size and resting cyst size-a structure with morphology that may reflect overall body size-we selected chrysophyte algae (Chrysophyceae) producing siliceous stomatocysts as a model. In this study, we obtained and identified 85 chrysophyte strains representing 31 Mallomonas species using nuclear ITS rDNA region barcoding and estimated their genome sizes using propidium iodide flow cytometry. Within this genus, we observed more than a 75-fold variation in genome size (0.15-11.25 pg) and frequent substantial intraspecific variation, in some cases consistent with whole-genome doubling (polyploidization). By summarizing the published genome size records, adding our new measurements and combining them with published cyst sizes, we assembled a data set for 54 chrysophyte species to examine the relationship between genome size and cyst volume. Linear regression on log-transformed data revealed a strong positive correlation ( <math> <mrow><msubsup><mi>R</mi> <mi>adj</mi> <mn>2</mn></msubsup> </mrow> </math> = 0.626, p < 0.001), showing that species with larger genomes tend to produce larger stomatocysts. In chrysophytes, this relationship is considerably stronger than the widely documented genome size-cell size correlation observed across eukaryotes. Our findings suggest that stomatocyst size is a more informative predictor of genome size in this group and highlight the potential for using stomatocyst sizes to infer ancestral genome sizes from the fossil record.","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":" ","pages":"1671-1682"},"PeriodicalIF":4.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13659389/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148684665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sugar kelp (Saccharina latissima) population genetics map onto geographic distance and oceanographic features across coastal Maine. 糖海带（Saccharina latissima）种群遗传学映射到缅因州沿海的地理距离和海洋学特征。

IF 4.3 3区生物学

Journal of Phycology Pub Date : 2026-10-01 Epub Date: 2026-08-15 DOI: 10.1111/jpy.70219

Rene D Francolini, Robin S Sleith, Kristina M Cammen, Damian C Brady, Douglas B Rasher

{"title":"Sugar kelp (Saccharina latissima) population genetics map onto geographic distance and oceanographic features across coastal Maine.","authors":"Rene D Francolini, Robin S Sleith, Kristina M Cammen, Damian C Brady, Douglas B Rasher","doi":"10.1111/jpy.70219","DOIUrl":"10.1111/jpy.70219","url":null,"abstract":"Sugar kelp (Saccharina latissima; order Laminariales) plays a vital role in kelp forest ecosystems, as well as an expanding kelp aquaculture industry, in the Gulf of Maine, United States. However, ocean warming is eroding the resilience of Maine's kelp forests and may be compromising their local genetic diversity, with impacts on population structure and gene flow. Here, we used genome-wide single nucleotide polymorphism (SNP) data to assess the genetic diversity, structure, and connectivity of S. latissima populations at 11 outer coastal sites spanning the historical range of kelp forests in Maine. Our analyses identified moderate genetic diversity and limited inbreeding within sites (average heterozygosity: 0.27). Further, they revealed that three clusters comprising four genetically distinct populations exist across the study region. Population structure was strongly associated with geographic distance and oceanographic features, as supported by principal coordinate analysis, FST calculations, Bayesian clustering, and spore dispersal modeling. Lastly, our outlier analysis identified genes potentially under selection. Thus, our findings highlight distinct, genetically unique kelp populations along Maine's coast and emphasize the need for regional management strategies that support both ecosystem resilience and sustainable aquaculture under climate change.","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":" ","pages":"1683-1701"},"PeriodicalIF":4.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148760889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Finding the sweet spot: An exploration of environmental factors to optimize Ulva carbohydrate content and composition. 寻找最佳点：探索优化Ulva碳水化合物含量和组成的环境因素。

IF 4.3 3区生物学

Journal of Phycology Pub Date : 2026-09-29 DOI: 10.1111/jpy.70231

Julie Grivotte, Zoë A Popper

引用次数: 0

Fletcheria gen. nov. (Ralfsiales, Phaeophyceae): A new crustose brown algal genus clarifying evolutionary relationships of Ralfsia pacifica. Fletcheria gen. 11 . (Ralfsiales, phaophyceae)：一个新的壳状褐藻属，阐明了Ralfsia pacifica的进化关系。

IF 4.3 3区生物学

Journal of Phycology Pub Date : 2026-09-29 DOI: 10.1111/jpy.70241

Viviana Patricia Reyes-Gómez, Manuela I Parente, Daniel León-Alvarez, Erasmo C Macaya, Gary W Saunders

{"title":"Fletcheria gen. nov. (Ralfsiales, Phaeophyceae): A new crustose brown algal genus clarifying evolutionary relationships of Ralfsia pacifica.","authors":"Viviana Patricia Reyes-Gómez, Manuela I Parente, Daniel León-Alvarez, Erasmo C Macaya, Gary W Saunders","doi":"10.1111/jpy.70241","DOIUrl":"10.1111/jpy.70241","url":null,"abstract":"The genus Ralfsia was historically broadly circumscribed but has recently undergone significant taxonomic revision. Based on molecular data, a restricted Ralfsia sensu stricto was delineated that had many species reassigned to new genera and families. The current work builds on that work by resolving the phylogenetic position, defining the morphological features, and establishing the biogeographic distribution of Ralfsia pacifica-a crustose brown alga widely distributed along the eastern Pacific coastline. We employed mitochondrial COI-5P and plastid rbcL gene markers, together with morpho-anatomical observations and comparisons with type material, to better understand this species and its distribution. Phylogenetic analyses positioned R. pacifica as a distinct lineage, sister to Neoralfsia within Neoralfsiaceae with moderate support. Significant molecular divergence from its nearest neighbor combined with a unique suite of morphological features-crustose thallus lacking clear differentiation of the cortex and medulla, frequent hair pits, and slightly elevated but largely nonadventitious plurangial sori-supported the establishment of Fletcheria gen. nov., with Fletcheria pacifica comb. nov. as the generitype, provisionally assigned to Neoralfsiaceae. The species was confirmed from British Columbia, Canada; California, United States; and central Chile, occupying intertidal habitats and reproducing year round with seasonal peaks. Based on morpho-anatomical observations, Ralfsia occidentalis is excluded from synonymy with F. pacifica. Anatomical features, although limited, place it closer to the genus Neoralfsia.","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":" ","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149075112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-wide association study of morphometric and metabolic characteristics in European populations of the sugar kelp Saccharina latissima. 欧洲糖海带种群形态计量学和代谢特征的全基因组关联研究。

IF 4.3 3区生物学

Journal of Phycology Pub Date : 2026-09-26 DOI: 10.1111/jpy.70239

Stéphane Mauger, Komlan Avia, Lucie Jaugeon, Paolo Ruggeri, Zofia Nehr, Ousseini Issaka Salia, Jérôme Coudret, Emilie Gouhier, Aurélien Baud, Stéphane Loisel, Antoine Fort, Ronan Sulpice, Christophe Destombe, Philippe Potin, J Mark Cock, Myriam Valero

{"title":"Genome-wide association study of morphometric and metabolic characteristics in European populations of the sugar kelp Saccharina latissima.","authors":"Stéphane Mauger, Komlan Avia, Lucie Jaugeon, Paolo Ruggeri, Zofia Nehr, Ousseini Issaka Salia, Jérôme Coudret, Emilie Gouhier, Aurélien Baud, Stéphane Loisel, Antoine Fort, Ronan Sulpice, Christophe Destombe, Philippe Potin, J Mark Cock, Myriam Valero","doi":"10.1111/jpy.70239","DOIUrl":"https://doi.org/10.1111/jpy.70239","url":null,"abstract":"The sugar kelp Saccharina latissima is a promising candidate for sustainable aquaculture in the North Atlantic and Northeast Pacific, but genetic improvement has been hindered by a limited understanding of the genetic basis of economically important traits. We conducted a genome-wide association study (GWAS) for this species using 202 self-fertilized pseudo-F1 individuals derived from 12 of 13 sampled populations spanning northern and southern European genetic clusters. Individuals were genotyped with ddRAD-seq-derived single nucleotide polymorphism markers and phenotyped in a common-garden experiment for four morphological traits (blade length, blade width, blade area, and stipe length) and six metabolic traits related to nitrogen metabolism. We identified 18 significant marker-trait associations, with individual loci explaining 1.37% to 10.93% of phenotypic variance (PVE). Rather than major-effect quantitative trait locus (QTL), the associated loci each accounted for a small-to-moderate proportion of trait variance, the largest being for nitrogen-metabolism traits (up to 10.93% PVE), and a single locus on chromosome 17 influenced both blade length and blade area. Marker-based heritability was high for morphological traits (0.75-0.99) but ranged widely for metabolic traits (0-0.99), with large standard errors throughout. Cross-validated genomic selection models yielded predictive abilities of 0.21-0.59 across traits, the only exception being for protein content, for which both heritability and predictive ability were effectively null. Overall, these results indicate a mixed genetic architecture, comprising moderate-effect loci amenable to marker-assisted selection and polygenic traits better suited to genomic selection, and they provide a foundation for genomics-assisted breeding in kelp aquaculture.","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":" ","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149062046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lithochloron, a new genus of green algae residing in coral skeleton (Cladophorales, Chlorophyta). 石绿藻：生长在珊瑚骨架上的绿藻新属（绿藻门，叶藻门）。

IF 4.3 3区生物学

Journal of Phycology Pub Date : 2026-09-24 DOI: 10.1111/jpy.70236

Heroen Verbruggen, Juntong Hu, Yuqun Du, Richard Wetherbee, Francesco Ricci, Manuel Poretti

{"title":"Lithochloron, a new genus of green algae residing in coral skeleton (Cladophorales, Chlorophyta).","authors":"Heroen Verbruggen, Juntong Hu, Yuqun Du, Richard Wetherbee, Francesco Ricci, Manuel Poretti","doi":"10.1111/jpy.70236","DOIUrl":"https://doi.org/10.1111/jpy.70236","url":null,"abstract":"Coral skeletons host diverse microbial communities, including endolithic green algae that contribute to holobiont functioning. The siphonous green alga Ostreobium (Bryopsidales) is arguably the best-known coral endolith, but recent environmental sequencing has indicated that other lineages of green algae may also inhabit the coral skeleton, including members of the Cladophorales. Here, we characterize a new endolithic green alga isolated from a Porites coral skeleton collected from Heron Island on the southern Great Barrier Reef. The alga forms uniseriate, sparsely branched grass-green filaments composed of elongated vegetative cells with infrequent cross walls. Phylogenetic analyses of 18S and 28S nrDNA gene sequences placed the strain as an early-diverging lineage within the Siphonocladus clade of the Cladophorales order of the Ulvophyceae. Based on morphological and molecular evidence, we describe the alga as the new genus and species Lithochloron capricornicum. Analysis of environmental 18S nrDNA gene amplicon data from Great Barrier Reef corals revealed extensive sequence diversity, suggesting the genus has additional yet undescribed species. Environmental sequencing further showed that it is most abundant at intermediate depths within the coral skeleton and suggested niche differentiation with Ostreobium along the vertical light gradient in the skeleton, with Lithochloron tending to peak at shallower depths than Ostreobium. The discovery of Lithochloron challenges the long-standing assumption that Ostreobium is the only dominant green algal endolith in coral skeletons and highlights the underestimated diversity of endolithic algae more broadly.","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":" ","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Circumscription of three new taxa of Merismopedia-like Cyanobacteria shows how far convergent evolution can go. 三种新分类群的边界似merismopedia蓝藻显示趋同进化可以走多远。

IF 4.3 3区生物学

Journal of Phycology Pub Date : 2026-09-18 DOI: 10.1111/jpy.70238

Jan Pokorný, Jan Kaštovský, Lenka Marešová Štenclová

{"title":"Circumscription of three new taxa of Merismopedia-like Cyanobacteria shows how far convergent evolution can go.","authors":"Jan Pokorný, Jan Kaštovský, Lenka Marešová Štenclová","doi":"10.1111/jpy.70238","DOIUrl":"https://doi.org/10.1111/jpy.70238","url":null,"abstract":"The genus Merismopedia (Merismopediaceae, Chroococcales), characterized by rectangular colonies with cells arranged in regular perpendicular rows and columns, represents a challenging study system due to its limited cultivability. The genus recently underwent taxonomic revision using cultivation-independent techniques and a polyphasic approach. Following the designation of an epitype, its type species, M. tranquilla was placed within a phylogenetic framework. Polyphyletic \"Merismopedia\" was split, and its species clustered within at least three distinct families. The case represents a clear example of convergent evolution, a phenomenon not uncommon among Cyanobacteria. The present study further explores the convergent evolution of Cyanobacteria with colonies exhibiting regularly perpendicular cell rows. Using predominantly a single-colony sequencing approach, partial 16S rRNA gene and ITS rRNA region sequence data were obtained for four additional Merismopedia morphotypes. Phylogenetic analyses supported the placement of two of these taxa within a recently described genus Eucapsopsis. Accordingly, two new species are proposed: Eucapsopsis complanata, distinguished by its loosely arranged cells, and Eucapsopsis sphagnicola, a new combination for Merismopedia sphagnicola, typically occurring in oligotrophic peat bogs. Furthermore, the description of a morphologically cryptic genus Cryptoplanktopedia is presented. A strain identified as \"Merismopedia\" cf. angularis NIVA-CYA 199, characterized by its L-shaped colonies, clustered as another distant lineage within Chroococcales although with ambiguous systematic placement. The results demonstrate that Merismopedia-like colony morphology is a widespread trait and has evolved convergently among Cyanobacteria.","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":" ","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148990455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extensive induction of diploid aposporous gametophytes and their application in polyploid breeding of Undaria pinnatifida. 二倍体无孢子配子体的广泛诱导及其在裙带菜多倍体育种中的应用。

IF 4.3 3区生物学

Journal of Phycology Pub Date : 2026-09-18 DOI: 10.1111/jpy.70242

Yuqian Li, Tifeng Shan

{"title":"Extensive induction of diploid aposporous gametophytes and their application in polyploid breeding of Undaria pinnatifida.","authors":"Yuqian Li, Tifeng Shan","doi":"10.1111/jpy.70242","DOIUrl":"https://doi.org/10.1111/jpy.70242","url":null,"abstract":"Triploid breeding is a promising strategy for developing Undaria pinnatifida cultivars with higher yield, better quality, and stronger resistance to environmental stresses. We previously developed a triploid breeding method through crossing aposporous gametophytes derived from doubled haploid sporophytes with haploid gametophytes. However, only one male diploid aposporous gametophyte line was obtained. In the present study, we extensively induced three kinds of aposporous gametophytes, namely MoAG, PaAG, and CAG, from sporophytes derived, respectively, from selfing of monoicous gametophytes, parthenogenesis of female gametophytes, and cross-fertilization. We found that all the MoAGs were diploid and monoicous, the PaAGs all female and either diploid or haploid, and the CAGs all monoicous and either diploid or haploid. Parentage analysis revealed that cross-fertilization predominated when crossing the monoicous aposporous gametophytes with female haploid gametophytes. Triploid sporophyte lines were extensively generated by crossing the diploid gametophytes with haploid gametophytes. Six out of 17 triploid lines exhibited significantly superior comprehensive economic traits after cultivation in the sea and are therefore promising candidate cultivars for larger scale cultivation. Tetraploid sporophytes were obtained through selfing of diploid monoicous gametophytes. This study lays a solid foundation for polyploid breeding and development of superior cultivars in U. pinnatifida.","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":" ","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149001994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

EBA-Net: A hybrid EfficientNetB3-BiLSTM-Attention model for species-level diatom classification. EBA-Net：一种用于种级硅藻分类的高效netb3 - bilstm -注意力混合模型。

IF 4.3 3区生物学

Journal of Phycology Pub Date : 2026-09-18 DOI: 10.1111/jpy.70240

Esen Damla Balo Utku, Banu Kutlu, Anil Utku

{"title":"EBA-Net: A hybrid EfficientNetB3-BiLSTM-Attention model for species-level diatom classification.","authors":"Esen Damla Balo Utku, Banu Kutlu, Anil Utku","doi":"10.1111/jpy.70240","DOIUrl":"https://doi.org/10.1111/jpy.70240","url":null,"abstract":"Species identification in diatoms is important for assessing water bodies, determining environmental quality, and analyzing biodiversity. Nonetheless, diatom classification has proven difficult due to intra-class variation, interclass similarity, and data imbalance in microscopic images. In this regard, we propose a new deep learning approach for species-level diatom classification. Various deep learning models, including traditional convolutional neural networks or CNNs (ResNet50, DenseNet121), state-of-the-art CNNs (EfficientNetB3, Xception, MobileNetV3), transformer-based architectures (ViT-B16, Swin-Tiny), and our proposed EBA-Net (EfficientNetB3-BiLSTM-Attention Network), were extensively compared under identical experimental settings. The EBA-Net integrates the advantages of convolutional feature extraction, sequence modeling, and an attention mechanism to improve classification accuracy. It first uses the EfficientNetB3 model as the backbone network to obtain higher-level features, followed by a BiLSTM layer to capture dependencies among the extracted features. Then, it applies the attention mechanism to the BiLSTM output to highlight the most informative features. Experimental results showed that the proposed EBA-Net model outperforms other models considered in the study, yielding 95.8% accuracy and 85.1% macro F1-score. In addition, Top-3 (98.9%) and Top-5 (99.6%) accuracies confirmed the efficiency of the proposed model for classifying objects from visually similar classes. An analysis of the model's interpretability was conducted using Gradient-weighted Class Activation Mapping, showing that the proposed model's attention is concentrated on relevant morphological features of diatoms. To the best of our knowledge, the suggested approach achieves state-of-the-art accuracy on the data set of diatoms used.","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":" ","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149001908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0