Journal of Pharmacopuncture最新文献

{"title":"Anxiolytic and Antianhedonic-like Effects of <i>Psidium guajava</i> Leaf in Alcohol-Withdrawn Mice.","authors":"Vanjarapu Harithadevi, Vijayapandi Pandy","doi":"10.3831/KPI.2024.27.3.234","DOIUrl":"10.3831/KPI.2024.27.3.234","url":null,"abstract":"<p><strong>Objectives: </strong>Alcohol withdrawal syndrome manifests through a range of symptoms, including anxiety and anhedonia, significantly affecting the quality of life of those affected. This study investigates the potential therapeutic effects of the methanolic extract of <i>Psidium guajava</i> leaves (MPG) on anxiety and anhedonia in Swiss albino female mice undergoing alcohol withdrawal.</p><p><strong>Methods: </strong>Four groups of mice underwent alcohol withdrawal, with one group undergoing saline withdrawal as a control. On the test day, behavioral assessments were conducted to evaluate anxiety and anhedonia. Groups I and II received sodium carboxymethylcellulose, Group III received diazepam, and Groups IV and V received varying oral doses of MPG.</p><p><strong>Results: </strong>The results indicate significant anti-anhedonic and anxiolytic effects of MPG. These effects were observed through changes in parameters measured in the Open Field test, Elevated Plus Maze test, Marble Burying test, and Sucrose Preference test. Mice treated with MPG displayed reduced anxiety-like behaviors and increased sucrose preference compared to untreated mice undergoing alcohol withdrawal.</p><p><strong>Conclusion: </strong>These findings suggest that <i>Psidium guajava</i> leaf extract may have therapeutic potential in alleviating anxiety and anhedonia associated with alcohol withdrawal. The observed effects indicate that MPG could serve as a promising adjunct therapy for managing alcohol withdrawal symptoms, thereby enhancing the overall well-being of individuals undergoing alcohol cessation.</p>","PeriodicalId":16769,"journal":{"name":"Journal of Pharmacopuncture","volume":"27 3","pages":"234-244"},"PeriodicalIF":1.2,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11439518/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142348870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"General Worker's Sleep Disturbances and the Degree of Cold-Heat Symptoms: a national cross-sectional survey.","authors":"Min Kyung Hyun","doi":"10.3831/KPI.2024.27.3.199","DOIUrl":"10.3831/KPI.2024.27.3.199","url":null,"abstract":"<p><strong>Objectives: </strong>Few studies have examined the impact of healthy sleep among general workers on individuals and society. Therefore, the status and risk factors of sleep disturbances among general workers were investigated. In addition, this study assessed the degree to which cold and heat symptoms are associated with sleep disturbances.</p><p><strong>Methods: </strong>A nationwide cross-sectional study was conducted through an online questionnaire focused on sleep disturbances of the general public in 2021. The degree of cold-heat pattern Identification (CHPI) of the general public was also surveyed. Descriptive statistics and multivariate logistic regression were used to derive the study results.</p><p><strong>Results: </strong>Data from 2,822 workers out of 3,900 valid questionnaires were analyzed. Approximately half of the respondents (49.93%) had sleep disturbances. Among the types of work, self-employed, two-shift work, and working more than 53 hours were associated with sleep disturbances. Sleep disturbances were positively associated with six cold and heat symptoms three cold symptoms (coldness of the abdomen, coldness of body, and pale face) and three heat symptoms (body feverishness, feverishness of the limbs, and drinking cold water).</p><p><strong>Conclusion: </strong>Customized policies to maintain healthy work are needed for self-employed work, two-shift work, and long working hours, which are risk factors for workers' sleep disturbances. In addition, medical personnel can effectively diagnose and treat sleep disturbances considering the worker's cold and heat symptoms.</p>","PeriodicalId":16769,"journal":{"name":"Journal of Pharmacopuncture","volume":"27 3","pages":"199-210"},"PeriodicalIF":1.2,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11439519/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142348838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial and Anti-Inflammatory Potential of <i>Euphorbia paralias</i> (L.): a bioprospecting study with phytoconstituents analysis.","authors":"Ahmed Mohamed Mohamed Youssef, Thabet Hasan Ahmad Althneibat, Doaa Ahmed Mohamed Maaty, Yasser Gaber","doi":"10.3831/KPI.2024.27.3.223","DOIUrl":"10.3831/KPI.2024.27.3.223","url":null,"abstract":"<p><strong>Objectives: </strong>The phytochemicals in the aerial parts of <i>Euphorbia paralias</i> (also known as Sea Spurge) and their anti-inflammatory and antimicrobial activities were investigated.</p><p><strong>Methods: </strong>The methanolic extract was characterized using GC-MS and HPLC techniques. The anti-inflammatory feature was estimated through a Human Red Blood Cell (HRBC) membrane stabilization technique, while the antimicrobial feature was evaluated by the disc diffusion agar technique, minimum bactericidal concentration, and minimum inhibitory concentration (MIC) <i>via</i> micro-broth dilution method.</p><p><strong>Results: </strong>The GC/MS results demonstrated the existence of various phytochemicals, such as n-hexadecenoic acid, cis-11-eicosenoic acid, and methyl stearate, recognized for their anti-inflammatory and antibacterial features. The similarity of the phytochemical composition with other <i>Euphorbia</i> species emphasizes the genus-wide similarity. The anti-inflammatory activity exhibited a noteworthy inhibitory effect comparable to the reference drug indomethacin. The extract's antimicrobial potential was tested against a range of microorganisms, demonstrating significant action against Gram-positive bacteria and <i>Candida albicans</i>. The quantification of total phenolics and flavonoids further supported the therapeutic potential of the extract.</p><p><strong>Conclusion: </strong>The methanolic extract from <i>E. paralias</i> emerges as a successful natural source of important active constituents with potential applications as anti-inflammatory and antimicrobial agents. This research provides a first step to valorize <i>Euphorbia paralias</i> insights as a source of worthwhile phytochemicals that have potential applications in the pharmaceutical industry.</p>","PeriodicalId":16769,"journal":{"name":"Journal of Pharmacopuncture","volume":"27 3","pages":"223-233"},"PeriodicalIF":1.2,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11439520/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142348869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Vitro Antioxidant Activity and Anticonvulsant Properties on Zebrafish PTZ-Induced Seizure Model of a <i>Tilia viridis</i> Aqueous Extract.","authors":"Elina Malén Saint Martin, Ayelen Morena Sosa, Carolina Soledad Martinez, Jimena Prieto, Carla Marrassini, Cecilia Beatriz Dobrecky, María Rosario Alonso, Claudia Anesini","doi":"10.3831/KPI.2024.27.3.211","DOIUrl":"10.3831/KPI.2024.27.3.211","url":null,"abstract":"<p><strong>Objectives: </strong><i>Tilia viridis</i> (Bayer) Simonk. (Malvaceae) is widely distributed in Argentina and employed for its tranquilizing properties. Other species of the genus (<i>Tilia europaea</i> L., <i>Tilia cordata</i> Mill., <i>Tilia platyphyllos</i> Scop.) have been traditionally used for the treatment of epilepsy. Epilepsy affects approximately 65 million people worldwide and is characterized by an imbalance between excitatory and inhibitory processes in the brain, leading to unpredictable, unprovoked, recurrent seizures. Current pharmacological interventions often present mild to moderately severe side effects. Epilepsy has been associated with oxidative and nitrative stress as well as neuroinflammation. Herbal medicine therapies may offer new treatment options with multi-target antioxidant and anticonvulsant effects for patients whose seizures remain uncontrolled, potentially providing cost-effective solutions for individuals worldwide suffering from uncontrolled epilepsy.The aim of this study was to demonstrate the anticonvulsant activity of a standardized <i>T. viridis</i> aqueous extract (TE).</p><p><strong>Methods: </strong>Study of the constituents of TE, TE's antioxidant and anticonvulsant activities and toxicity, and analysis of the possible relation between the potential activities and the compounds present in the extract. In order to demonstrate TE's anticonvulsant activity a zebrafish model was used. The study also assessed TE's toxicity and antioxidant activity. To standardize the extract, total polyphenols and flavonoids were quantified and specific flavonoids were identified and quantified using HPLC-MS/MS and HPLC-UV.</p><p><strong>Results: </strong>TE exhibited anticonvulsant activity at low concentrations and demonstrated antioxidant effects by scavenging free radicals, exhibiting superoxide dismutase and peroxidase-like activities, as well as inhibiting lipoperoxidation. These actions can be attributed to the presence of polyphenols, particularly flavonoids.</p><p><strong>Conclusion: </strong>TE holds promise as a complementary herbal medicine in the treatment of epilepsy and may also offer benefits for other neuropathies associated with oxidative stress, such as Parkinson's disease and Alzheimer's disease.</p>","PeriodicalId":16769,"journal":{"name":"Journal of Pharmacopuncture","volume":"27 3","pages":"211-222"},"PeriodicalIF":1.2,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11439512/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142348839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Computational Evaluation on the Interactions of an Opaque-Phase ABC Transporter Associated with Fluconazole Resistance in <i>Candida albicans</i>, by the <i>Psidium guajava</i> Bio-Active Compounds.","authors":"Mithil Vora, Smiline Girija Aseervatham Selvi, Shoba Gunasekaran, Vijayashree Priyadharsini Jayaseelan","doi":"10.3831/KPI.2024.27.2.91","DOIUrl":"10.3831/KPI.2024.27.2.91","url":null,"abstract":"<p><strong>Objectives: </strong><i>Candida albicans</i> is an opportunistic pathogen that occurs as harmless commensals in the intestine, urogenital tract, and skin. It has been influenced by a variety of host conditions and has now evolved as a resistant strain. The aim of this study was thus detect the fluconazole resistant <i>C. albicans</i> from the root caries specimens and to computationally evaluate the interactions of an opaque-phase ABC transporter protein with the <i>Psidium guajava</i> bio-active compounds.</p><p><strong>Methods: </strong>20 carious scrapings were collected from patients with root caries and processed for the isolation of <i>C. albicans</i> and was screened for fluconazole resistance. Genomic DNA was extracted and molecular characterization of <i>Cdrp1</i> and <i>Cdrp2</i> was done by PCR amplification. <i>P. guajava</i> methanolic extract was checked for the antifungal efficacy against the resistant strain of <i>C. albicans</i>. Further <i>in-silico</i> docking involves retrieval of ABC transporter protein and ligand optimization, molinspiration assessment on drug likeness, docking simulations and visualizations.</p><p><strong>Results: </strong>65% of the samples showed the presence of <i>C.albicans</i> and 2 strains were fluconazole resistant. Crude methanolic extract of <i>P. guajava</i> was found to be promising against the fluconazole resistant strains of <i>C. albicans</i>. <i>In-silico</i> docking analysis showed that Myricetin was a promising candidate with a high docking score and other drug ligand interaction scores.</p><p><strong>Conclusion: </strong>The current study emphasizes that bioactive compounds from <i>Psidium guajava</i> to be a promising candidate for treating candidiasis in fluconazole resistant strains of <i>C. albicans</i> However, further <i>in-vivo</i> studies have to be implemented for the experimental validation of the same in improving the oral health and hygiene.</p>","PeriodicalId":16769,"journal":{"name":"Journal of Pharmacopuncture","volume":"27 2","pages":"91-100"},"PeriodicalIF":1.2,"publicationDate":"2024-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11194528/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141468884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toxicity Evaluation of a Non-Pain Pharmacopuncture Extract Using a Bacterial Reverse Mutation Test.","authors":"Ji Hye Hwang, Chul Jung","doi":"10.3831/KPI.2024.27.2.154","DOIUrl":"10.3831/KPI.2024.27.2.154","url":null,"abstract":"<p><strong>Objectives: </strong>The objective of this study was to assess the genotoxicity of a no-pain pharmacopuncture (NPP) extract developed in 2022 using a bacterial reverse mutation assay, aiming to further substantiate the safety profile of NPP.</p><p><strong>Methods: </strong>The genotoxicity evaluation involved a bacterial reverse mutation assay to assess the mutagenic potential of NPP extracts with and without metabolic activation. Histidine-requiring <i>Salmonella typhimurium</i> strains (TA98, TA100, TA1535, and TA1537) and tryptophan-requiring <i>Escherichia coli</i> strains (WP2uvrA) were used in the assay.</p><p><strong>Results: </strong>The NPP extract did not induce a revertant colony count exceeding two times that of the negative control at any dose level in any of the tested strains, both with and without metabolic activation. Additionally, no growth inhibition or precipitation was observed in the presence of NPP.</p><p><strong>Conclusion: </strong>Based on the findings, it can be concluded that the NPP extract exhibited no mutagenic potential in the <i>in vitro</i> genotoxicity tests conducted.</p>","PeriodicalId":16769,"journal":{"name":"Journal of Pharmacopuncture","volume":"27 2","pages":"154-161"},"PeriodicalIF":1.2,"publicationDate":"2024-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11194521/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141468890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome Analysis of the Striatum of Electroacupuncture-treated Naïve and Ischemic Stroke Mice.","authors":"Hong Ju Lee, Hwa Kyoung Shin, Ji-Hwan Kim, Byung Tae Choi","doi":"10.3831/KPI.2024.27.2.162","DOIUrl":"10.3831/KPI.2024.27.2.162","url":null,"abstract":"<p><strong>Objectives: </strong>Electroacupuncture (EA) has been demonstrated to aid stroke recovery. However, few investigations have focused on identifying the potent molecular targets of EA by comparing EA stimulation between naïve and disease models. Therefore, this study was undertaken to identify the potent molecular therapeutic mechanisms underlying EA stimulation in ischemic stroke through a comparison of mRNA sequencing data obtained from EA-treated naïve control and ischemic stroke mouse models.</p><p><strong>Methods: </strong>Using both naïve control and middle cerebral artery occlusion (MCAO) mouse models, EA stimulation was administered at two acupoints, Baihui (GV20) and Dazhui (GV14), at a frequency of 2 Hz. Comprehensive assessments were conducted, including behavioral evaluations, RNA sequencing to identify differentially expressed genes (DEGs), functional enrichment analysis, protein-protein interaction (PPI) network analysis, and quantitative real-time PCR.</p><p><strong>Results: </strong>EA stimulation ameliorated the ischemic insult-induced motor dysfunction in mice with ischemic stroke. Comparative analysis between control vs. MCAO, control vs. control + EA, and MCAO vs. MCAO + EA revealed 4,407, 101, and 82 DEGs, respectively. Of these, 30, 7, and 1 were common across the respective groups. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses revealed upregulated DEGs associated with the regulation of inflammatory immune response in the MCAO vs. MCAO + EA comparison. Conversely, downregulated DEGs in the control vs. control + EA comparison were linked to neuronal development. PPI analysis revealed major clustering related to the regulation of cytokines, such as <i>Cxcl9</i>, <i>Pcp2</i>, <i>Ccl11</i>, and <i>Cxcl13</i>, in the common DEGs of MCAO vs. MCAO + EA, with <i>Esp8l1</i> identified as the only common downregulated DEG in both EA-treated naïve and ischemic models.</p><p><strong>Conclusion: </strong>These findings underscore the diverse potent mechanisms of EA stimulation between naïve and ischemic stroke mice, albeit with few overlaps. However, the potent mechanisms underlying EA treatment in ischemic stroke models were associated with the regulation of inflammatory processes involving cytokines.</p>","PeriodicalId":16769,"journal":{"name":"Journal of Pharmacopuncture","volume":"27 2","pages":"162-171"},"PeriodicalIF":1.2,"publicationDate":"2024-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11194526/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141468891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of Herbal Decoction and Pharmacopuncture in Individuals with Chronic Disease: findings from a nationally representative panel.","authors":"Chan-Young Kwon, Sunghun Yun, Bo-Hyoung Jang, Il-Su Park","doi":"10.3831/KPI.2024.27.2.110","DOIUrl":"10.3831/KPI.2024.27.2.110","url":null,"abstract":"<p><strong>Objectives: </strong>This study analyzed the Korea Health Panel Annual Data 2019 to investigate factors related to the use of non-insured Korean medicine (KM) treatment in individuals with chronic diseases. The non-insured KM treatments of interest were herbal decoction (HD) and pharmacopuncture (PA).</p><p><strong>Methods: </strong>Among adults aged 19 or older, 6,159 individuals with chronic diseases who received outpatient KM treatment at least once in 2019 were included. They were divided into three groups according to the KM treatment used (1) basic insured KM non-pharmacological treatment (BT) group (n = 629); (2) HD group (n = 256); (3) PA group (n = 184). Logistic regression analysis was used to explore factors associated with favoring HD or PA use over BT. Potentially relevant candidate factors were classified using the Andersen Behavior Model.</p><p><strong>Results: </strong>Compared to BT, the 1st to 3rd quartiles of income compared to the 4th quartile (odds ratio 1.50 to 2.06 for HD; 2.03 to 2.83 for PA), health insurance subscribers compared to medical aid (odds ratio 2.51; 13.43), and presence of musculoskeletal diseases (odds ratio 1.66; 1.91) were significantly positively associated with HD and PA use. Moreover, the presence of cardiovascular disease (odds ratio 1.46) and neuropsychiatric disease (odds ratio 1.97) were also significantly positively associated with HD use.</p><p><strong>Conclusion: </strong>The presence of some chronic diseases, especially musculoskeletal diseases, was significantly positively associated with HD and PA use, while low economic status was significantly negatively associated with HD and PA use, indicating the potential existence of unmet medical needs in this population. Since chronic diseases impose a considerable health burden, the results of this study can be used for reference for future health insurance coverage policies in South Korea.</p>","PeriodicalId":16769,"journal":{"name":"Journal of Pharmacopuncture","volume":"27 2","pages":"110-122"},"PeriodicalIF":1.2,"publicationDate":"2024-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11194527/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141468892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overcoming Barriers to Research Competency: a nationwide mixed-method study on residency training in the field of Korean medicine.","authors":"Min-Jung Lee, Myung-Ho Kim","doi":"10.3831/KPI.2024.27.2.142","DOIUrl":"10.3831/KPI.2024.27.2.142","url":null,"abstract":"<p><strong>Objectives: </strong>This study aimed to analyze the educational needs of interns and residents in Korean medicine as the first step in developing an education program to improve their research competencies.</p><p><strong>Methods: </strong>A mixed-method design, incorporating both quantitative and qualitative data collection methods, was used to investigate the educational needs for research competencies among interns and residents working in Korean medicine hospitals nationwide. Data were collected through online surveys and online focus group discussions (FGDs), and processed using descriptive statistical analysis and thematic analysis. The study results were derived by integrating survey data and FGD outcomes.</p><p><strong>Results: </strong>In total, 209 interns and residents participated in the survey, and 11 individuals participated in two rounds of FGDs. The majority of participants felt a lack of systematic education in research and academic writing in postgraduate medical education and highlighted the need for nationally accessible education due to significant disparities in the educational environment across hospitals and specialties. The primary barrier to learning research and academic writing identified by learners was the lack of knowledge, leading to time constraints. Improving learners' research competencies, relationship building, autonomy, and motivation through a support system was deemed crucial. The study also identified diverse learner types and preferred educational topics, indicating a demand for learner-centered education and coaching.</p><p><strong>Conclusion: </strong>This study provides foundational data for designing and developing a program on education on research competencies for interns and residents in Korean medicine and suggests the need for initiatives to strengthen these competencies.</p>","PeriodicalId":16769,"journal":{"name":"Journal of Pharmacopuncture","volume":"27 2","pages":"142-153"},"PeriodicalIF":1.2,"publicationDate":"2024-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11194522/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141468886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Pandanus amaryllifoius</i> Roxb. Leaves Ethanol Extract Ameliorates Lipid and Proinflammatory Cytokines Profiles in a Rat Model of Dyslipidemia.","authors":"Martohap Parotua Lumbanraja, Kusnandar Anggadiredja, Neng Fisheri Kurniati, Hubbi Nashrullah Muhammad","doi":"10.3831/KPI.2024.27.2.101","DOIUrl":"10.3831/KPI.2024.27.2.101","url":null,"abstract":"<p><strong>Objectives: </strong>Dyslipidemia has currently become a major health challenge that still opens for safer and more effective modes of treatment. The plant <i>Pandanus amaryllifolius</i> Roxb. (pandan) has been indicated to contain active ingredients that interfere with the pathological pathway of dyslipidemia. The aim of the study was to test the effects of pandan leaves ethanol extract on lipid and proinflammatory profiles in a rat dyslipidemic model.</p><p><strong>Methods: </strong>Dyslipidemia was induced by administration of high-fat feed for 8 weeks. Treatments (vehicle, the reference drug simvastatin at 1.8 mg/kg, and extract at 200, 300 or 600 mg/kg) were given for 4 weeks following the completion of induction.</p><p><strong>Results: </strong>Significant post-treatment decreases in total cholesterol, low density lipoprotein (LDL), and triglyceride levels in groups receiving all doses of extract and simvastatin were observed. Similar results were also found in regards to proinflammatory cytokines levels. Pandan extracts significantly lowered the concentrations of IL-6, TNF-α, and NFκB p65. Characterization of metabolite contents of the extract confirmed the presence of the previously suggested active alkaloids pandamarilactonine-A and B.</p><p><strong>Conclusion: </strong>Taken together, results of the present study implied the ameliorating effects of pandan leaves ethanol extract in dyslipidemic condition which is potential for opening an avenue in combating this essential component of metabolic disorder.</p>","PeriodicalId":16769,"journal":{"name":"Journal of Pharmacopuncture","volume":"27 2","pages":"101-109"},"PeriodicalIF":1.2,"publicationDate":"2024-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11194525/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141468883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}