Journal of Pharmacopuncture最新文献

{"title":"Characterization of Vancomycin Resistant <i>Enterococci</i> and Drug Ligand Interaction between <i>vanA</i> of <i>E. faecalis</i> with the Bio-Compounds from <i>Aegles marmelos</i>.","authors":"Jayavarsha V,&nbsp;Smiline Girija A S,&nbsp;Shoba Gunasekaran,&nbsp;Vijayashree Priyadharsini J","doi":"10.3831/KPI.2023.26.3.247","DOIUrl":"10.3831/KPI.2023.26.3.247","url":null,"abstract":"Objectives Enterococcus faecalis is a gram positive diplococci, highly versatile and a normal commensal of the gut microbiome. Resistance to vancomycin is a serious issue in various health-care setting exhibited by vancomycin resistant Enterococci (VRE) due to the alteration in the peptidoglycan synthesis pathway. This study is thus aimed to detect the VRE from the patients with root caries from the clinical isolates of E. faecalis and to evaluate the in-silico interactions between vanA and the Aegles marmelos bio-compounds. Methods E. faecalis was phenotypically characterized from 20 root caries samples and the frequency of vanA and vanB genes was detected by polymerase chain reaction (PCR). Further crude methanolic extracts from the dried leaves of A. marmelos was assessed for its antimicrobial activity. This is followed by the selection of five A. marmelos bio-compounds for the computational approach towards the drug ligand interactions. Results 12 strains (60%) of E. faecalis was identified from the root caries samples and vanA was detected from two strains (16%). Both the stains showed the presence of vanA and none of the strains possessed vanB. Crude extract of A. marmelos showed promising antibacterial activity against the VRE strains. In-silico analysis of the A. marmelos bio-compounds revealed Imperatonin as the best compound with high docking energy (–8.11) and hydrogen bonds with < 140 TPSA (Topological polar surface area) and zero violations. Conclusion The present study records the VRE strains among the root caries with imperatorin from A. marmelos as a promising drug candidate. However the study requires further experimentation and validation.","PeriodicalId":16769,"journal":{"name":"Journal of Pharmacopuncture","volume":"26 3","pages":"247-256"},"PeriodicalIF":1.4,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/6e/34/jop-26-3-247.PMC10547814.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41136693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acupuncture as an Additional Method of Rehabilitation Post-COVID-19: a randomized controlled trial.","authors":"Indira Omarova, Assiya Akanova, Almagul Kurmanova, Gaukhar Kurmanova, Natalya Glushkova, Amina Seidanova, Kuatzhan Turysbekov","doi":"10.3831/KPI.2023.26.3.238","DOIUrl":"10.3831/KPI.2023.26.3.238","url":null,"abstract":"<p><strong>Objectives: </strong>The purpose of this study was to evaluate the effectiveness of complex rehabilitation with and without acupuncture in a hospital setting.</p><p><strong>Methods: </strong>A randomized clinical trial was performed at Rehabilitation center \"Kamenskoe Plato\" in Almaty, Kazakhstan. 160 patients with Post COVID-19 condition were randomly equally divided into an acupuncture with complex rehabilitation methods and a only complex rehabilitation methods group in the period from March 1, 2022 to July 1, 2022. Either groups was performed for an 10-14 days period. The outcome measures were the Bartel index, the Borg scale, Modified Dyspnea Scale and the 6-minute walking test. Adverse events also were monitored and documented.</p><p><strong>Results: </strong>We found statistically significant improvement after the rehabilitation course with acupuncture in the all scales. And in the group without acupuncture, only on two scales MDS and Borg scale.</p><p><strong>Conclusion: </strong>Rehabilitation with acupuncture is possible and effective in patients recovering from post-COVID-19. Our findings may be useful to guide clinicians taking care of patients with post-COVID-19.</p>","PeriodicalId":16769,"journal":{"name":"Journal of Pharmacopuncture","volume":"26 3","pages":"238-246"},"PeriodicalIF":1.2,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/65/61/jop-26-3-238.PMC10547817.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41120771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial Activity of Selected Fruit Juices against Multidrug-Resistant Bacterial Pathogens Involved in Urinary Tract and Sexually Transmitted Infections among Tribal Women in Madhya Pradesh, India.","authors":"Poonam Sharma,&nbsp;Juhi,&nbsp;Vaishali Halwai,&nbsp;Sainivedita Rout,&nbsp;Rambir Singh","doi":"10.3831/KPI.2023.26.3.265","DOIUrl":"10.3831/KPI.2023.26.3.265","url":null,"abstract":"<p><strong>Objectives: </strong>The aim of this study was to evaluate the effect of fruit juices on Multi-Drug Resistant (MDR) bacterial pathogens involved in Urinary Tract Infections (UTIs) and Sexually Transmitted Infections (STIs) among tribal women in the district Anuppur, Madhya Pradesh, India.</p><p><strong>Methods: </strong>Fresh juices of lemon (<i>Citrus limon</i>), amla/Indian gooseberry (<i>Phyllanthus emblica</i>), pineapple (<i>Ananas comosus</i>), mosambi/sweet lime (<i>Citrus limetta</i>), orange (<i>Citrus sinensis</i>), kiwi (<i>Actinidia deliciosa</i>), and pomegranate (<i>Punica granatum</i>) fruits were evaluated for in vitro antibacterial activity against bacterial pathogens involved in UITs and STIs among tribal women. Physico-chemical analysis of fresh fruits was also carried out by measuring the pH, moisture, protein, fat, crude fibre, carbohydrate, and ascorbic acid content.</p><p><strong>Results: </strong>Lemon and amla juice showed better antibacterial activity against the pathogens as compared to other juices. MIC results fruit juices against UTIs and STIs pathogens vary depending on the specific pathogen and juice chemical constituents. The physico-chemical analysis showed that the moisture content was highest in mosambi (90%), followed by orange (87%). Ascorbic acid content was found highest in amla (540 mg/100 g), followed by kiwi (90.3 mg/100 g). Pomegranate showed highest concentration of carbohydrate (15.28 g/100 g), fat (1.28 g/100 g), and protein (1.65 g/100 g). Lemon juice had lowest pH of 2.20, followed by amla 2.67.</p><p><strong>Conclusion: </strong>The lemon juice showed highest antibacterial activity against MDR bacterial pathogens involved in UTIs and STIs among tribal women in district Anuppur, Madhya Pradesh, India. The low pH of lemon may be responsible for its high antibacterial activity as compared to other juices.</p>","PeriodicalId":16769,"journal":{"name":"Journal of Pharmacopuncture","volume":"26 3","pages":"265-275"},"PeriodicalIF":1.4,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/1e/b9/jop-26-3-265.PMC10547819.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41133958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effects of Acupuncture on Crohn's Disease: a systematic review and meta-analysis.","authors":"Joon Hyun Bae,&nbsp;Seo Young Kang,&nbsp;Si Eun You,&nbsp;Hye In Jeong,&nbsp;Soobin Jang,&nbsp;Kyeong Han Kim","doi":"10.3831/KPI.2023.26.3.211","DOIUrl":"10.3831/KPI.2023.26.3.211","url":null,"abstract":"<p><strong>Objectives: </strong>Crohn's disease is a chronic gastrointestinal disease that belongs to inflammatory bowel disease. This systematic review aims to assess the level of evidence in randomized controlled trials (RCTs) on the effects of acupuncture for Crohn's disease.</p><p><strong>Methods: </strong>We searched 12 databases from the date of the establishment of each database up to May, 2023 for relevant RCTs. The risk of bias of each study was assessed independently by three reviewers. The level of evidence of meta-analysis was assessed using GRADE (Grading of Recommendations, Assessment, Development, and Evaluation).</p><p><strong>Results: </strong>A total of 12 studies were included. The effective rate (odds ratio [OR] 3.23, 95% confidence interval [CI] 1.43, 7.30) for mild to moderate Crohn's disease patients showed a significant difference between the acupuncture with moxibustion group and the sham-acupuncture with sham-moxibustion group. CDAI change (mean difference [MD] -74.15, 95% CI -93.28, -55.01) for mild to moderate Crohn's disease showed a significant difference between the acupuncture with moxibustion group and the sham-acupuncture with sham-moxibustion group.</p><p><strong>Conclusion: </strong>Although acupuncture with moxibustion showed significant effects compared to sham-acupuncture with sham-moxibustion, the effect of acupuncture alone is inconclusive. Moreover, only the effect of acupuncture treatment on mild to moderate Crohn's disease patients was derived as a remarkable result. To confirm the effectiveness of acupuncture treatment for Crohn's disease, studies using only acupuncture for intervention or more RCTs targeting various Crohn's disease patients according to the CDAI are required.</p>","PeriodicalId":16769,"journal":{"name":"Journal of Pharmacopuncture","volume":"26 3","pages":"211-226"},"PeriodicalIF":1.4,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/44/28/jop-26-3-211.PMC10547815.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41099581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acupuncture for Attenuating Frontal Lobe α Band Asymmetry Induced by Anger: a pilot study.","authors":"Sung-A Kim,&nbsp;Yujin Choi,&nbsp;Seung-Hun Cho","doi":"10.3831/KPI.2023.26.3.276","DOIUrl":"10.3831/KPI.2023.26.3.276","url":null,"abstract":"<p><strong>Objectives: </strong>Previous studies have shown that anger can lead to frontal lobe α (8-13 Hz) band asymmetry (FAA) in electroencephalogram (EEG), in accordance with motivational direction. This pilot study aimed to investigate the impact of acupuncture on FAA elicited by anger.</p><p><strong>Methods: </strong>Thirty-four right-handed participants scoring above 75 points on the Novaco Anger Scale were included. Baseline EEG signals were recorded for eight minutes using a 32-channel cap under comfortable conditions. Anger was induced through a nine-minute sequence of Articulated Thoughts in Simulated Situations (ATSS) task. Following that, participants received acupuncture at GB20 and GB21 for 10 minutes. Fast Fourier transform was employed for frequency analysis, and repeated measure ANOVA was conducted for statistical analysis.</p><p><strong>Results: </strong>The results revealed that participants exhibited significantly higher FAA (p = 0.026), particularly in the left hemisphere, after the ATSS task sequence compared to the baseline. During acupuncture treatment, the greater left-sided FAA was significantly reduced (p = 0.027) and reversed. Upon the cessation of acupuncture, FAA returned to a value between the baseline and the anger-evoked stage (p = 0.046).</p><p><strong>Conclusion: </strong>The EEG results of this study revealed that anger stimulation induced an increase in left-sided FAA, which was effectively alleviated by acupuncture. This led to an immediate restoration of FAA asymmetry induced by anger. These findings suggest the potential of acupuncture as a treatment option for reducing FAA associated with anger.</p>","PeriodicalId":16769,"journal":{"name":"Journal of Pharmacopuncture","volume":"26 3","pages":"276-284"},"PeriodicalIF":1.4,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/50/ed/jop-26-3-276.PMC10547816.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41136243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>In Silico</i> Studies of Indole Derivatives as Antibacterial Agents.","authors":"Mridul Shah,&nbsp;Adarsh Kumar,&nbsp;Ankit Kumar Singh,&nbsp;Harshwardhan Singh,&nbsp;Balasubramanian Narasimhan,&nbsp;Pradeep Kumar","doi":"10.3831/KPI.2023.26.2.147","DOIUrl":"https://doi.org/10.3831/KPI.2023.26.2.147","url":null,"abstract":"<p><strong>Objectives: </strong>Molecular docking and QSAR studies of indole derivatives as antibacterial agents.</p><p><strong>Methods: </strong>In this study, we used a multiple linear regressions (MLR) approach to construct a 2D quantitative structure activity relationship of 14 reported indole derivatives. It was performed on the reported antibacterial activity data of 14 compounds based on theoretical chemical descriptors to construct statistical models that link structural properties of indole derivatives to antibacterial activity. We have also performed molecular docking studies of same compounds by using Maestro module of Schrodinger. A set the molecular descriptors like hydrophobic, geometric, electronic and topological characters were calculated to represent the structural features of compounds. The conventional antibiotics sultamicillin and ampicillin were not used in the model development since their structures are different from those of the created compounds. Biological activity data was first translated into pMIC values (i.e. -log MIC) and used as a dependent variable in QSAR investigation.</p><p><strong>Results: </strong>Compounds with high electronic energy and dipole moment were effective antibacterial agents against <i>S. aureus</i>, indole derivatives with lower κ₂ values were excellent antibacterial agents against MRSA standard strain, and compounds with lower R value and a high ²χ^v value were effective antibacterial agents against MRSA isolate.</p><p><strong>Conclusion: </strong>Compounds 12 and 2 showed better binding score against penicillin binding protein 2 and penicillin binding protein 2a respectively.</p>","PeriodicalId":16769,"journal":{"name":"Journal of Pharmacopuncture","volume":"26 2","pages":"147-157"},"PeriodicalIF":1.4,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/31/12/jop-26-2-147.PMC10315882.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9802265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"External Treatment of Pyritum for Musculoskeletal Trauma: a protocol for systematic review.","authors":"Ji Hye Hwang,&nbsp;Su Hyun Choi","doi":"10.3831/KPI.2023.26.2.192","DOIUrl":"https://doi.org/10.3831/KPI.2023.26.2.192","url":null,"abstract":"<p><strong>Objectives: </strong>Musculoskeletal trauma is a common type of injury that can result from damage to the muscular or skeletal system and has been recognized as a leading cause of death and disability worldwide. This study aims to analyze the efficacy of Pyritum external treatment for musculoskeletal trauma.</p><p><strong>Methods: </strong>Randomized controlled trials evaluating the external treatment effect of Pyritum on various types of musculoskeletal traumatic injuries will be considered and identified in the searches of eight databases from their inception to Feb 2023. There will be no restrictions with respect to the publication status, language, or country. The experimental intervention group will be treated with external application of Pyritum alone or in combination with other therapies, and the comparator intervention group will include all types of control interventions. The primary outcome will be measured as treatment efficacy rate, and secondary outcomes will include pain reduction, pain disappearance time, swelling, joint function, and recovery period. Assessment of the methodological quality of this study will be concluded using the risk of bias assessment recommended by the Cochrane Collaboration. If there are sufficient numbers of studies per group in terms of specific rating scales to compare the treatment effects of Pyrium alone and combined external treatment groups, we will consider subgroup analysis.</p><p><strong>Results: </strong>This systematic review will be conducted in compliance with the PRISMA-P statement.</p><p><strong>Conclusion: </strong>We will conduct an extensive search on the proposed topic within the available literature and provide systematic evidence for the efficacy and safety of external application of Pyritum for all types of musculoskeletal trauma. The evidence generated will help design interventions for the external use of Pyritum for this patient group.</p>","PeriodicalId":16769,"journal":{"name":"Journal of Pharmacopuncture","volume":"26 2","pages":"192-197"},"PeriodicalIF":1.4,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/11/a2/jop-26-2-192.PMC10315878.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9793260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modulation of Immunological, Biochemical, and Histopathological Changes of Airway Remodeling by <i>Withania somnifera</i> in an Experimental Model of Allergic Asthma in Rats.","authors":"Nafaa Hasan Ali,&nbsp;Sana Rehman,&nbsp;Maaz Naqvi,&nbsp;Kavita Gulati,&nbsp;Arunabha Ray","doi":"10.3831/KPI.2023.26.2.158","DOIUrl":"https://doi.org/10.3831/KPI.2023.26.2.158","url":null,"abstract":"<p><strong>Objectives: </strong>Airway remodeling in asthma involves chronic inflammation associated with structural changes, which result in severe airflow limitation and very few therapeutic options. Thus, the present study was designed to experimentally evaluate the ameliorative effects of <i>Withania somnifera</i> (WS) root extract against Ovalbumin (OVA)-induced airway remodeling in a rat model of asthma.</p><p><strong>Methods: </strong>Wistar rats were immunized (i.p) and challenged (aerosol) with ovalbumin (OVA), and the effects of WS extract were investigated on the development and progress of airway remodeling by assessing immunological, biochemical, and histological changes in these rats.</p><p><strong>Results: </strong>OVA-immunization and challenge in rats resulted in significant increases in the levels of IL-13, 8-OhdG, TGF-β, hydroxyproline, and periostin in bronchoalveolar lavage fluid (BALF) and serum/lung homogenate compared to normal control (saline only) rats, and these changes were attenuated after WS extract (200 and 400 mg/kg), as well as dexamethasone (DEX, 1 mg/kg) pretreatments. Further, WS extract attenuated histopathological changes and maintained lung integrity. In herb-drug interactions, sub-threshold doses of WS extract and DEX showed synergistic effects on all parameters studied as compared to either form of monotherapy.</p><p><strong>Conclusion: </strong>These results indicated that WS exerted significant protective effects against airway remodeling in the experimental model by modulating inflammatory and fibrotic cytokines, and could have the potential for developing a therapeutic alternative/adjunct for the treatment of airway remodeling of bronchial asthma.</p>","PeriodicalId":16769,"journal":{"name":"Journal of Pharmacopuncture","volume":"26 2","pages":"158-166"},"PeriodicalIF":1.4,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/98/23/jop-26-2-158.PMC10315884.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9793267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>In vitro</i> Antibacterial Activity and Wound Healing Effects of <i>Achillea millefolium</i> Essential Oil in Rat.","authors":"Mohammad Reza Ghasemi,&nbsp;Akram Ranjbar,&nbsp;Pari Tamri,&nbsp;Shabnam Pourmoslemi,&nbsp;Alireza Nourian,&nbsp;Dara Dastan","doi":"10.3831/KPI.2023.26.2.167","DOIUrl":"https://doi.org/10.3831/KPI.2023.26.2.167","url":null,"abstract":"<p><strong>Objectives: </strong>In this study we aimed to evaluate the <i>in vitro</i> antibacterial activity and wound healing properties of <i>Achillea millefolium</i> essential oil (AMEO) in full-thickness wound model in rat. The antibacterial activity of AMEO was evaluated against <i>Staphylococcus aureus</i> and <i>Pseudomonas aeruginosa</i> using the broth dilution method.</p><p><strong>Methods: </strong>The 2 cm × 2 cm full-thickness excisional wounds were created on the back of animals. Topical therapy was applied twice a day using 1%, 2%, and 3% w/w AMEO ointments, and the measurement of the wounds area was carried out every 3 days, after that the wound closure percentage was calculated in these days. Hydroxyproline content and histopathological evaluation of wound tissue samples were carried out on day 7 and 14 post wounding. Eucerin was used for the treatment of vehicle control group and negative control group received no treatment.</p><p><strong>Results: </strong>Our results revealed the bacteriostatic activity of AMEO against <i>S. aureus</i> and <i>P. aeruginosa</i>. Wound healing activity evaluation of AMEO showed the significant increase (p < 0.05) in the wound closure percentages in rats treated with AMEO 1% and 2% comparing to those of non-treatment group. In addition, hydroxyproline contents of tissue significantly (p < 0.01) increased in AMEO 1% and 2% comparing to non-treatment group. Histopathological evaluations of wound tissue samples on day 7 and 14 demonstrated higher accumulation of collagen fibers, reduction of edema and inflammation and also formation of tissue appendages in 1% and 2% AMEO treated groups in comparison with non-treatment group.</p><p><strong>Conclusion: </strong>The results of this study indicated that AMEO has the potential to be used as a safe and effective wound healing agent.</p>","PeriodicalId":16769,"journal":{"name":"Journal of Pharmacopuncture","volume":"26 2","pages":"167-174"},"PeriodicalIF":1.4,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/b7/a2/jop-26-2-167.PMC10315885.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10178735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial and Anti-Glucosyltransferase Activity of <i>Verbascum speciosum</i> Against Cariogenic Streptococci.","authors":"Shabnam Pourmoslemi,&nbsp;Amir Larki-Harchegani,&nbsp;Sajad Daneshyar,&nbsp;Dara Dastan,&nbsp;Amir Nili-Ahmadabadi,&nbsp;Mina Jazaeri","doi":"10.3831/KPI.2023.26.2.139","DOIUrl":"https://doi.org/10.3831/KPI.2023.26.2.139","url":null,"abstract":"<p><strong>Objectives: </strong>Dental caries is a prevalent chronic human infection worldwide and several plants have shown anticariogenic properties through antibacterial activity against oral pathogens. The present study aimed to assess anticariogenic activity of <i>Verbascum speciosum</i>, in search of novel agents for the prevention and treatment of dental caries.</p><p><strong>Methods: </strong>Hydro-alcoholic extracts from flowers and total aerial parts of the plant were prepared by maceration. Antibacterial activity of the extracts against <i>Streptococcus mutans</i> (ATCC 35668) and <i>Streptococcus sobrinus</i> (ATCC 27607) was investigated by agar diffusion and microdilution techniques. Inhibitory concentration-fifty values of the flowers' extract against <i>Streptococcus mutans</i> glucosyltransferase enzymes were determined. The total flavonoid content of the extracts was determined using an aluminum chloride reaction.</p><p><strong>Results: </strong><i>Verbascum speciosum</i> flowers' extract showed significantly higher flavonoid content and antibacterial activity; with minimum inhibitory concentrations of 100 and 200 µg/mL for <i>Streptococcus mutans</i> and <i>Streptococcus sobrinus</i>, respectively. The extract inhibited the synthesis of glucan by cell-associated and extracellular glucosyltransferase enzymes in a dose-dependent manner with higher activity against the extracellular enzyme.</p><p><strong>Conclusion: </strong>This study indicated effective anticariogenic activity of Verbascum speciosum flowers extract. This extract can be considered as an alternative to current anticaries therapies or an additive to dental care products.</p>","PeriodicalId":16769,"journal":{"name":"Journal of Pharmacopuncture","volume":"26 2","pages":"139-146"},"PeriodicalIF":1.4,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/36/92/jop-26-2-139.PMC10315880.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10178737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}