{"title":"Isolation and identification of Sphingomonas paucimobilis 503: a case report","authors":"M. Ibrahim, Noha Awwad, D. Talat, H. Ellakany","doi":"10.21608/djvs.2022.142767.1078","DOIUrl":"https://doi.org/10.21608/djvs.2022.142767.1078","url":null,"abstract":"","PeriodicalId":166243,"journal":{"name":"Damanhour Journal of Veterinary Sciences","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132472751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Elbehiry, Mostafa Mahboob, M. Marey, S. Hattab, R. Ghallab, R. Dohreig
{"title":"Efficacy of three hormonal treatments for cystic ovarian disease and subsequent reproductive performance in dairy cows","authors":"M. Elbehiry, Mostafa Mahboob, M. Marey, S. Hattab, R. Ghallab, R. Dohreig","doi":"10.21608/djvs.2021.99394.1053","DOIUrl":"https://doi.org/10.21608/djvs.2021.99394.1053","url":null,"abstract":"Cystic ovarian disease (COD) is a common ovarian disorder that causes major reproductive failure in dairy cows leading to significant economic losses in the dairy industry. This study aimed to evaluate the efficacy and the response of cows with ovarian cysts to three different treatments regardless of the cyst type. At 50 days postpartum, were considered to have an ovarian cyst when possessing a follicle with a diameter ≥ 17 mm in the absence of a corpus luteum in two ultrasonographic examinations performed at 7 days intervals. In the second examination considered (Day 0), 51 cystic Holstein cows were assigned randomly into one of 3 treatment groups.; GnRH group ( n =12) treated with 100 μg gonadorelin as a single dose, GP group ( n =12) received (100 μg gonadorelin on day 0 followed by 500 μg of cloprostenol sodium on day 7, CIDR group ( n =12) treated with intravaginal progesterone-releasing insert CIDR on the day 0: and 100 μg gonadorelin then an injection of 500 µg cloprostenol sodium IM on day 7. The CIDR was removed on day 8, and a second GnRH injection was given on day 9. From day 9, animals were observed for estrus and inseminated with proven frozen-thawed semen. Control Group ( n =15) represents cows that did not receive any treatment. Pregnancy was confirmed by ultrasound on day 28 post-insemination. Results showed that the GP group achieved the highest estrus induction rate (EIR). Thus, 44.4% of treated cows come in estrus less than 10 days and 100 % by day 20. While GnRH group showed EIR with 25% in less than 10 days and 62.5 % at the day 20. The CIDR group showed EIR (0%) in less than 10 days and 100 % at the day 20. Total conception rate (3 cycles after treatment) GP recorded (88.9%) GnRH group (87.5%) and CIDR (85.7%), while pregnancy rates (PR) was highest (66.7%) in GP protocol compared to GnRH (58.3%), and CIDR (49.9%). In conclusion, our results showed that the (GP group) had the best reproductive performance (earliest and highest estrus induction rate and highest pregnancy rate after treatment regardless of the type of the cyst.","PeriodicalId":166243,"journal":{"name":"Damanhour Journal of Veterinary Sciences","volume":"46 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128297765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of Dietary Supplementation of Some Emulsifiers on Growth Performance, Carcass Traits, Lipid Peroxidation and Some Nutrients Digestibility in Broiler Chickens","authors":"Samah A. Ganna, M. Abdel-Latif, H. Ahmed","doi":"10.21608/djvs.2022.121880.1066","DOIUrl":"https://doi.org/10.21608/djvs.2022.121880.1066","url":null,"abstract":"For Five weeks feeding trail, one hundred and eighty one day old chicks were used to investigate the effect of dietary supplementation of some emulsifiers on growth performance, carcass traits in broiler chickens and some nutrients digestibility in broiler. The birds were randomly divided into Six groups, each group were divided into Three replicate (Ten birds per replicate) and fed the experimental diets; group1 (G1) fed on the basal diet without additives as control, group 2 (G2) fed basal diet with bile acids(Bas), group 3 (G3) fed basal diet with Bile acids and Lecithin (L), group 4 (G4) fed basal diet with bile acids and polyethylene glycol ricinoleate (PGR), group 5 (G5) fed basal diet with lecithin and group 6 (G6) fed basal diet with polyethylene glycol ricinoleate. The results showed nonsignificant (p≥0.05) differences in final body weight, total weight gain and total feed conversion ratio between all experimental groups but the results of total feed intake showed significant (p<0.05) increase in bile, bile with lecithin and lecithin supplemented groups compared with the control one. Liver index showed significant (p<0.05) increase in bile group while bile with PGR group showed significant (p<0.05) decrease compared with the control one. Abdominal fat showed significant (p<0.05) increase in bile and bile with PGR groups. The obtained results of lipid peroxidation (malondialdehyde content) in both liver and breast muscle meat showed nonsignificant (p≥0.05) differences among all experimental groups. Poly unsaturated fatty acid content in meat showed nonsignificant (p≥0.05) difference between all experimental groups but there was a numerical increase in all treated groups compared with the control one. The results of dry matter, protein and fat digestibility showed nonsignificant (p≥0.05) differences between all experimental groups.","PeriodicalId":166243,"journal":{"name":"Damanhour Journal of Veterinary Sciences","volume":"90 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123980083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Molecular detection of some virulence genes in multidrug resistant Salmonella species isolated from chicken meat products and raw milk","authors":"E. Saleh, Hanan El-Lawendy, E. Khedr, Eman Ali","doi":"10.21608/djvs.2022.234975","DOIUrl":"https://doi.org/10.21608/djvs.2022.234975","url":null,"abstract":"Salmonella still has a serious foodborne outbreak with public health risk. Chicken meat, chicken meat products, and raw milk are important reservoir for Salmonella. In the current study, a total of 120 samples of chicken meat and chicken meat products (breast, thigh, giblets, frozen thigh, nuggets, burger, shish and luncheon, 15 of each) and 50 raw cow milk samples were tested for prevalence, serotyping, virulence genes, and antibiotic resistance profiles of Salmonella spp.. The prevalence of Salmonella spp. was 11.67% in chicken meat samples with the following incidence on each sample group; 13.33, 20, 26.67, 0, 6.67, 6.67, 13.33 and 6.67%, respectively. Raw milk samples overall occurrence of Salmonella spp. was 6%. Serological identification of the isolated Salmonella revealed presence of five different serotypes including S. kentukay, S. entritides, S. typhimurium, S. lindenberg and S . bassa. All isolated Salmonella spp. har-bored stn gene; while S. kentukay, S. entritides and S. typhimurium harbored mgtC gene but invA was found in S. entritides, S. typhimurium, S. lindenberg and S. bassa. Also, sopB was detected in S. kentukay, S. entritides, S. typhimurium and S. lindenberg. The isolated Salmonella spp. was resistant to sulphamethoxazol trimethoprim, chloramphenicol, and penicillin (100%). Meanwhile, the sensitivity was 70.6% % to ampicillin, enrofloxacin and amoxycillin clavulanic. The results confirm the importance of application of strict hygienic measures in food industry and proper use of antibiotics for meat and milk producing animals.","PeriodicalId":166243,"journal":{"name":"Damanhour Journal of Veterinary Sciences","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125443231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mervat Elbarbary, Amin A. Elamin, W. Darwish, Asmaa B. M. B. Tahoun
{"title":"Effect of probiotic bacteria on the reduction of aflatoxin risks in milk","authors":"Mervat Elbarbary, Amin A. Elamin, W. Darwish, Asmaa B. M. B. Tahoun","doi":"10.21608/djvs.2022.139623.1071","DOIUrl":"https://doi.org/10.21608/djvs.2022.139623.1071","url":null,"abstract":"Dairy products such as raw milk, Kariesh cheese, and yoghurt are considered is considered as health food that supports part of the human needs of protein, minerals, and vitamins. This study was taken to investigate mold contamination in raw milk, Kariesh cheese, and yoghurt retailed in Zagazig city, Egypt. In addition, isolation and identification of the different mold genera were further conducted. Besides, total aflatoxins (AFTs) were estimated in the examined samples, and their daily intakes and health risk assessment were calculated. An experimental trial was conducted to investigate the antifungal activities of three probiotics strains, namely, Lactobacillus acidophilus, L. rhamnosus, and L. plantarum. The obtained results indicated that raw milk had the highest mold contamination rate at 40%, followed by Kariesh cheese at 30%, and yoghurt samples at 10%. The prevalent mold genera were Aspergillus spp., Penicillium spp., Cladosporium spp., Alternaria spp., Mucor spp., Rhizopus spp., Absidia spp., and Fusarium spp. The most dominant mold genera among the identified molds were Aspergillus spp., (raw milk (20.82%), Kariesh cheese (15.62%), and yoghurt (6.25%). Further identification of the Aspergillus isolates revealed five Aspergillus species. The identified Aspergilli were A. niger, A. flavus, A. fumigatous. A. ochracous, and A. terreus. AFTs were detected in raw milk samples at 20%, Kariesh cheese at 10%, and yoghurt at 6. None of the contaminated samples exceeded the international permissible limit of total AFTs. The tested probiotics strains exhibited clear antifungal activities.","PeriodicalId":166243,"journal":{"name":"Damanhour Journal of Veterinary Sciences","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130556741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Nawar, Mohamed El-sherif, A. Sobhy, Hazem Shaheen, M. Kassem
{"title":"Echocardiographic and Biochemical Assessments Following combinations of Acepromazine-Ketamine and Xylazine-Ketamine Anesthesia in Dogs","authors":"M. Nawar, Mohamed El-sherif, A. Sobhy, Hazem Shaheen, M. Kassem","doi":"10.21608/djvs.2022.139435.1070","DOIUrl":"https://doi.org/10.21608/djvs.2022.139435.1070","url":null,"abstract":"The present study investigated echocardiographic and biochemical changes following ketamine anesthesia induction in dogs sedated with acepromazine (0.2 mg/kg) or xylazine (3 mg/kg). Seven dogs of both sexes were assigned to this experiment with a mean weight of 20 ± 4.8 kg. Fifteen minutes after administering the acepromazine as preanesthetic medication, ketamine (3 mg/kg) was administered to 7 dogs in the group: Acepromazine -ketamine (AK). Systolic blood pressure (SBP) was measured, and echocardiography was performed immediately before application of the sedative protocol (M0), 15 minutes after the sedation (M1), and immediately after anesthesia induction (M2). The second anesthetic protocol was performed one month later with the same seven dogs; group: xylazine-ketamine (XK). Non-significant differences were observed in SBP and hemodynamic variables such as cardiac index, shortening fraction, and ejection fraction, between groups at all time points evaluated (M0, M1, and M2). The SBP was significantly reduced after anesthetic induction in dogs in the AK group. It can be concluded that; both AK and XK protocols similarly reduce the SBP in dogs subjected to anesthetic induction. All anesthetic induction protocols maintained a stable CI in all pre-medicated dogs. None of the two anesthetic protocols which were evaluated promoted significant echocardiographic changes. Furthermore, ketamine and xylazine combination hurt myocardial function. On the other hand, due to their cardiac depressant effects, acepromazine and xylazine anesthetic integration should be applied with attention in ill cases.","PeriodicalId":166243,"journal":{"name":"Damanhour Journal of Veterinary Sciences","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132910851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sara Elbeshlawy, Rania Hamada, W. Goda, A. Abou-Rawash, Shahinaz N. Hassan
{"title":"Protective Role of Concanavalin-A and/or IL-27 Against Adverse Effects of Cyclophosphamide on Hematological and Biochemical Parameters in Tumor-Bearing Mice","authors":"Sara Elbeshlawy, Rania Hamada, W. Goda, A. Abou-Rawash, Shahinaz N. Hassan","doi":"10.21608/djvs.2022.141297.1076","DOIUrl":"https://doi.org/10.21608/djvs.2022.141297.1076","url":null,"abstract":"Cyclophosphamide (CTX) is a chemotherapeutic drug used to treat mammary gland tumors. Despite its cytotoxic effect on cancer cells, it causes harm to hepatic and renal tissues, ranging from mild infiltration to necrosis and finally cytolysis, depending on the dosage and duration of treatment. It also suppresses bone marrow, resulting in leukopenia, anemia, and thrombocytopenia. This study aimed to evaluate the protective effects of concanavalin A (con-A) and IL-27 conditioned immune therapeutics on Ehrlich ascites carcinoma (EAC) tumor-bearing mice treated with CTX. To achieve this, naive splenocyte cells were activated in vitro for 3 days with the T cell mitogen concanavalin A (Con-A; 5 µg/mL) and/or IL-27 (10; ng/mL). After a single i/p injection of CTX (4 mg/mouse), they were adoptively transferred into (EAC) tumor-bearing mice. After tumor implantation, hematological analysis, liver function, and kidney function were evaluated. According to the findings, the CTX-treated group had significantly lower total leucocyte count, lymphocytes, neutrophils, hematocrit, and RBCs than the control group. Interestingly, Con-A + IL-27 normalized the total leucocytes' count, especially lymphocytes and neutrophils. When Con-A + IL-27 was compared to CTX, there was a significant increase in hematocrit and platelet count. AST, ALT, bilirubin, creatinine, and urea levels were not significantly different between normal and Con-A + IL-27, but CTX significantly increased AST compared to normal. According to the findings, adoptive transfer of Con-A + IL-27 conditioned splenocyte cells into lymphopenic hosts restored hematological and biochemical parameters and recovery from chemotherapy-induced lymphopenia.","PeriodicalId":166243,"journal":{"name":"Damanhour Journal of Veterinary Sciences","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128818745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Multiple Natural and Hormonal Methods Improve Reproductive and Productive Performance of Naturally Mated Multiparous Rabbit Does","authors":"M. Younis","doi":"10.21608/djvs.2022.154980.1086","DOIUrl":"https://doi.org/10.21608/djvs.2022.154980.1086","url":null,"abstract":"This experiment illustrates the impact of various natural and hormonal methods on the reproductive and productive performance of naturally mated multiparous rabbit does. 180 multiparous does were randomly and equally allotted on six experimental treatments (1) DLS (doe litter separation); (2) DG (does gathering), (3) CC (cage change), (4) OP100 (estradiol 27 µg and progesterone 270 µg/kg body weight), (5) OP300 (estradiol 80 µg and progesterone 800 µg/kg body weight), and (6) control group, the experiment was repeated on three-time points (2, 7, and 14 days postpartum). All used treatments, either natural or hormonal, induced significant positive effects on receptivity and fertility compared with control treatment, with the superiority of the natural methods to hormonal one. Although DLS group significantly decreased average fryer weaning weight, OP300 group increased it significantly compared with the control group. All experimental treatments achieved significantly higher weaning numbers than the control. It’s better to apply these methods on days 2 and 7 postpartum than on day 14 postpartum. It concluded that the CC and DG methods are effective in improving multiparous rabbit production. However, these treatments must only be applied to healthy herds as animal contact may serve as a source of contamination. Moreover, estrogen and progesterone can be used as good hormonal methods for improvement of rabbit production although further studies are necessary to determine how it affects the other blood hormones and whether prolonged usage of these hormones can impair ovarian function and trigger an immunological response.","PeriodicalId":166243,"journal":{"name":"Damanhour Journal of Veterinary Sciences","volume":"40 6","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120906819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Heba Waheeb, Soad Menshawy, S. Mahmoud, Y. Otify, M. AbouLaila
{"title":"Prevalence and scanning electron microscope of some parasites infecting domesticated and migratory quails from Edko and Rashid districts, El-Behera governorate, Egypt","authors":"Heba Waheeb, Soad Menshawy, S. Mahmoud, Y. Otify, M. AbouLaila","doi":"10.21608/djvs.2022.236994","DOIUrl":"https://doi.org/10.21608/djvs.2022.236994","url":null,"abstract":"Quails have many advantages over other poultry species. Its meat has achieved great popularity as an excellent source of protein and other important nutrients. However, there are some limitations to quails production. One of them is the susceptibility to parasitic diseases that cause severe economic losses. Therefore, this work aimed to determine the infection rate and morphology of parasites infecting quails in ElBehera governorate, Egypt. 100 quails (50 migratory, Coturnix coturnix japonica and 50 domesticated quails) were collected. The gastrointestinal tracts of each bird were examined to collect helminths. Fecal materials were examined by direct and flotation methods to detect any coccidian species. The results showed that the total percentage of infection with parasites was 55%. The prevalence of parasitic infection in migratory and domesticated quails was 40% and 70%, respectively. Two species of helminths were recorded, Raillitina tetragona and Heterakis gallinarum with a prevalence of 87.5% and 22.22%, respectively. The morphology of helminths was described using a scanning electron microscope. Eimeria bateri, Eimeria tsunodai, and Eimeria uzura were among the protozoa identified. The histopathological changes in infected tissue with Eimeria species were recorded. In conclusion, this study presented the parasites’ prevalence, morphology, and histopathological changes in infected tissue with Eimeria species in examined domesticated and migratory quails.","PeriodicalId":166243,"journal":{"name":"Damanhour Journal of Veterinary Sciences","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129017304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Temperature impacts on Growth Performance, Hormonal Changes and Hematological Parameters of African Catfish (Clarias Jarpinus","authors":"Emad Zidan","doi":"10.21608/djvs.2022.132172.1069","DOIUrl":"https://doi.org/10.21608/djvs.2022.132172.1069","url":null,"abstract":"This take a look at become directed to discover the effect of temperature on increase and survival rate of African catfish. Different temperatures as 24, 27, 30, 32, 34C and 36C had been examined and 27C become a control. Plasma cortisol, plasma glucose, osmotic strain, and increase overall performance parameters had been envisioned at some point of the experimental duration of 25 days. The outcomes discovered that increase and survival rate had been prompted with the aid of using excessive temperature stage. Moreover, cortisol, glucose, and osmotic strain had been without delay proportional to the temperature stage. Therefore, it become concluded that multiplied temperature stage impacts increase and survival and different hormones of African catfish. So that a few growth in water temperature might appear like useful to catfish subculture overall performance.","PeriodicalId":166243,"journal":{"name":"Damanhour Journal of Veterinary Sciences","volume":"40 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128233004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}