Journal of microscopy最新文献_第8页

Application of various microscopy techniques to study early-age and longer-term behaviour of super sulphated cement microstructure 应用各种显微镜技术研究超硫酸盐水泥微观结构的早期龄期和长期行为。

IF 2 4区工程技术

Journal of microscopy Pub Date : 2024-03-02 DOI: 10.1111/jmi.13281

Migueli Chedrewih, Marta Medala, Christelle Schmid, Emmanuel Garcia, Denis Damidot, Vincent Thiéry

{"title":"Application of various microscopy techniques to study early-age and longer-term behaviour of super sulphated cement microstructure","authors":"Migueli Chedrewih, Marta Medala, Christelle Schmid, Emmanuel Garcia, Denis Damidot, Vincent Thiéry","doi":"10.1111/jmi.13281","DOIUrl":"10.1111/jmi.13281","url":null,"abstract":"Super sulphated cement (SSC) is a very promising substitute for traditional construction materials (i.e. Portland cement), due to its enhanced durability and particularly low environmental impact. This paper explores the microstructure and certain properties of SSC, focusing on the particular complexities of its microstructure and the difficulties of microanalysis of its hydrates. To do so, SSC paste samples were first cast to identify hydration products using X-ray diffraction, then observed at early age using confocal laser scanning microscopy (CLSM) and at early and late age using scanning electron microscopy. In addition, concrete cores impregnated with fluorescein in order to highlight porosity, cracking and aggregates debonding were observed under UV light using optical microscopy (OM), showing a complete absence of cracking and aggregate debonding. Both microscopy techniques (CLSM and UV light OM) have been applied to this type of binder for the first time. The results show that SSC microstructure is characterised by a sophisticated intergrowth of various phases, including ettringite and amorphous calcium-(alumina)-silicate hydrate gels. Finally, Monte–Carlo simulation of electron-matter has been provided for a better understanding of EDS analysis.","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140012717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of the SMLM technique and the MSSR algorithm in confocal microscopy for super-resolved imaging of cellulose fibres. 比较共聚焦显微镜中用于纤维素纤维超分辨成像的 SMLM 技术和 MSSR 算法。

IF 2 4区工程技术

Journal of microscopy Pub Date : 2024-02-29 DOI: 10.1111/jmi.13287

Josué David Hernández-Varela, Susana Dianey Gallegos-Cerda, José Jorge Chanona-Pérez, Liliana Edith Rojas Candelas, Eduardo Martínez-Mercado

{"title":"Comparison of the SMLM technique and the MSSR algorithm in confocal microscopy for super-resolved imaging of cellulose fibres.","authors":"Josué David Hernández-Varela, Susana Dianey Gallegos-Cerda, José Jorge Chanona-Pérez, Liliana Edith Rojas Candelas, Eduardo Martínez-Mercado","doi":"10.1111/jmi.13287","DOIUrl":"https://doi.org/10.1111/jmi.13287","url":null,"abstract":"Nowadays, the use of super-resolution microscopy (SRM) is increasing globally due to its potential application in several fields of life sciences. However, a detailed and comprehensive guide is necessary for understanding a single-frame image's resolution limit. This study was performed to provide information about the structural organisation of isolated cellulose fibres from garlic and agave wastes through fluorophore-based techniques and image analysis algorithms. Confocal microscopy provided overall information on the cellulose fibres' microstructure, while techniques such as total internal reflection fluorescence microscopy facilitated the study of the plant fibres' surface structures at a sub-micrometric scale. Furthermore, SIM and single-molecule localisation microscopy (SMLM) using the PALM reconstruction wizard can resolve the network of cellulose fibres at the nanometric level. In contrast, the mean shift super-resolution (MSSR) algorithm successfully determined nanometric structures from confocal microscopy images. Atomic force microscopy was used as a microscopy technique for measuring the size of the fibres. Similar fibre sizes to those evaluated with SIM and SMLM were found using the MSSR algorithm and AFM. However, the MSSR algorithm must be cautiously applied because the selection of thresholding parameters still depends on human visual perception. Therefore, this contribution provides a comparative study of SRM techniques and MSSR algorithm using cellulose fibres as reference material to evaluate the performance of a mathematical algorithm for image processing of bioimages at a nanometric scale. In addition, this work could act as a simple guide for improving the lateral resolution of single-frame fluorescence bioimages when SRM facilities are unavailable.","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139990341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Microstructural and micromechanical characteristics of composite osteoconductive coatings deposited by the atmospheric pressure plasma technique 利用常压等离子体技术沉积的复合骨传导涂层的微结构和微机械特性。

IF 1.5 4区工程技术

Journal of microscopy Pub Date : 2024-02-28 DOI: 10.1111/jmi.13285

Lukasz Major, D. F. Kopp, R. Major, Jürgen Markus Lackner

{"title":"Microstructural and micromechanical characteristics of composite osteoconductive coatings deposited by the atmospheric pressure plasma technique","authors":"Lukasz Major, D. F. Kopp, R. Major, Jürgen Markus Lackner","doi":"10.1111/jmi.13285","DOIUrl":"10.1111/jmi.13285","url":null,"abstract":"Long-term placement of facial implants requires avoiding the formation of fibrous tissue capsules around the artificial material by creating osteoconductive properties of the surface. Most promising approach is the deposition coatings made of materials very similar to bone mineral components, that is, calcium phosphates such as hydroxyapatite (HAp). As part of the research work, an innovative, cost-effective atmospheric pressure plasma deposition (APPD) system was used as a low-temperature coating technology for generating the HAp coatings deposition. Full microstructural characterisation of the coatings using SEM and TEM techniques was carried out in the work. It has been shown that the fully crystalline HAp powder undergoes a transformation during the coatings deposition and the material had a quasi-sintered structure after deposition. The crystalline phase content increased at the coating/substrate interface, while the surface of the HAp was amorphous. This is a very beneficial phenomenon due to the process of bioresorption. The amorphous phase undergoes much faster biodegradation than the crystalline one. In order to increase the bioactivity of the HAp, Zn particles were introduced on the surface of the coating. The TEM microstructural analysis in conjunction with the qualitative analysis of the EDS chemical composition showed that the binding of the Zn particles within the HAp matrix had diffusive character, which is very favourable from the point of view of the quality of the adhesion and the bioactivity of the coating. In the case of such a complex structure and due to its very porous nature, micromechanical analysis was carried out in situ in SEM, that is, by microhardness measurements of both the HAp matrix and the Zn particle. It was shown that the average value of HAp microhardness was 4.395 GPa ± 0.08, while the average value of Zn microhardness was 1.142 GPa ± 0.02","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139983163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Early hydration of C4AF with silica fume and its role on katoite composition C4 AF 与硅灰的早期水化及其对卡托石成分的影响。

IF 2 4区工程技术

Journal of microscopy Pub Date : 2024-02-28 DOI: 10.1111/jmi.13280

Martin Boháč, Anežka Zezulová, Michaela Krejčí Kotlánová, Dana Kubátová, Theodor Staněk, Zdeněk Krejza, Dalibor Všianský, Radek Novotný, Jakub Palovčík, Karel Dvořák

{"title":"Early hydration of C4AF with silica fume and its role on katoite composition","authors":"Martin Boháč, Anežka Zezulová, Michaela Krejčí Kotlánová, Dana Kubátová, Theodor Staněk, Zdeněk Krejza, Dalibor Všianský, Radek Novotný, Jakub Palovčík, Karel Dvořák","doi":"10.1111/jmi.13280","DOIUrl":"10.1111/jmi.13280","url":null,"abstract":"C4AF is considered the least reactive main clinker phase, but its reactivity may be affected by adding supplementary cementitious materials (SCMs). Pure C4AF was synthesised in a laboratory furnace, and the role of silica fume without gypsum on its early hydration properties was monitored. Burning was carried out in four stages to achieve 99% purity of C4AF. Heat flow development was monitored by isothermal calorimetry over 7 days of hydration at 20°C and 40°C. The role of silica fume on hydrogarnet phase katoite (Ca3Al2(SiO4)3 – x(OH)4x x = 1.5–3) formation during early hydration was studied. Rapid dissolution of C4AF, formation of metastable C-(A,F)-H and its conversion to C3(A, F)H6 was evidenced by isothermal calorimetry as a large exotherm. Changes in microstructure during early hydration were documented by SE micrographs, EDS point analyses, X-ray mapping and line scans by SEM-EDS. The phase composition was characterised by DTA-TGA and QXRD after 7 days of hydration. The katoite diffraction pattern is similar for the reference sample and sample with silica fume, but substitution in its structure can be revealed by X-ray microanalyses. The composition of katoite is variable due to the various extent of substitution of 4OH− by SiO44− due to silica fume.","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139990342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Python tool to determine the thickness of the hydrate layer around clinker grains using SEM-BSE images 利用 SEM-BSE 图像确定熟料颗粒周围水合物层厚度的 Python 工具。

IF 2 4区工程技术

Journal of microscopy Pub Date : 2024-02-26 DOI: 10.1111/jmi.13283

Florian Kleiner, Franz Becker, Christiane Rößler, Horst-Michael Ludwig

{"title":"A Python tool to determine the thickness of the hydrate layer around clinker grains using SEM-BSE images","authors":"Florian Kleiner, Franz Becker, Christiane Rößler, Horst-Michael Ludwig","doi":"10.1111/jmi.13283","DOIUrl":"10.1111/jmi.13283","url":null,"abstract":"To accurately simulate the hydration process of cementitious materials, understanding the growth rate of C-S-H layers around clinker grains is crucial. Nonetheless, the thickness of the hydrate layer shows substantial variation around individual grains, depending on their surrounding. Consequently, it is not feasible to measure hydrate layers manually in a reliable and reproducible manner. To address this challenge, a software has been developed to statistically determine the C-S-H thickness, requiring minimal manual interventions for thresholding and for setting limits like particle size or circularity.This study presents a tool, which automatically identifies suitable clinker grains and and perform statistical measurements of their hydrate layer up to a specimen age of 28 days. The findings reveal a significant increase in the C-S-H layer, starting from 0.45 <math>\u0000 <semantics>\u0000 <mrow>\u0000 <mi>μ</mi>\u0000 <mi>m</mi>\u0000 </mrow>\u0000 <annotation>$umu {rm {m}}$</annotation>\u0000 </semantics></math> after 1 day and reaching 3.04 <math>\u0000 <semantics>\u0000 <mrow>\u0000 <mi>μ</mi>\u0000 <mi>m</mi>\u0000 </mrow>\u0000 <annotation>$umu {rm {m}}$</annotation>\u0000 </semantics></math> after 28 days. However, for older specimens, the measurement of the C-S-H layer was not feasible due to limited pore space and clinker grains.","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jmi.13283","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139972241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigations on lightweight concrete by in situ compression tests using high-resolution computed tomography (μ-CT) 利用高分辨率计算机断层扫描（μ-CT）对轻质混凝土进行现场压缩试验研究。

IF 2 4区工程技术

Journal of microscopy Pub Date : 2024-02-24 DOI: 10.1111/jmi.13279

Cristin Umbach, Bernhard Middendorf

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More than just ‘added value’: The perils of not establishing shared core facilities in resource-constrained communities 不仅仅是 "附加值"：在资源有限的社区不建立共享核心设施的危险。

IF 2 4区工程技术

Journal of microscopy Pub Date : 2024-02-20 DOI: 10.1111/jmi.13277

Mai A. Rahmoon, Chad M. Hobson, Jesse S. Aaron, Harikrushnan Balasubramanian, Teng-Leong Chew

{"title":"More than just ‘added value’: The perils of not establishing shared core facilities in resource-constrained communities","authors":"Mai A. Rahmoon, Chad M. Hobson, Jesse S. Aaron, Harikrushnan Balasubramanian, Teng-Leong Chew","doi":"10.1111/jmi.13277","DOIUrl":"10.1111/jmi.13277","url":null,"abstract":"The accelerating pace of technological advancements necessitates specialised expertise and cutting-edge instruments to maintain competitive research in life sciences. Core facilities – collaborative laboratories equipped with state-of-the-art tools and staffed by expert personnel – are vital resources that support diverse scientific endeavours. However, their adoption in lower-income communities has been comparatively stagnant due to both financial and cultural challenges. This paper explores the perils of not supporting core facilities on national research enterprises, underscoring the need for balanced investments in discovery science and crucial infrastructure support. We explore the implications from the perspectives of funders, university leaders and lab heads. We advocate for a paradigm shift to recognise these facilities as essential components of national research efforts. Core facilities are positioned not as optional but as strategic investments that can catalyse breakthroughs, particularly in environments with limited resources.","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jmi.13277","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139905849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GaN atomic electric fields from a segmented STEM detector: Experiment and simulation 来自分段 STEM 探测器的氮化镓原子电场：实验与模拟

IF 1.5 4区工程技术

Journal of microscopy Pub Date : 2024-02-19 DOI: 10.1111/jmi.13276

Tim Grieb, Florian F. Krause, Thorsten Mehrtens, Christoph Mahr, Beeke Gerken, Marco Schowalter, Bert Freitag, Andreas Rosenauer

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TOC - Issue Information TOC - 发行信息

IF 2 4区工程技术

Journal of microscopy Pub Date : 2024-02-19 DOI: 10.1111/jmi.13196

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Unravelling molecular dynamics in living cells: Fluorescent protein biosensors for cell biology. 揭示活细胞中的分子动力学：用于细胞生物学的荧光蛋白生物传感器。

IF 1.5 4区工程技术

Journal of microscopy Pub Date : 2024-02-15 DOI: 10.1111/jmi.13270

Colline Sanchez, Andrea Ramirez, Louis Hodgson

{"title":"Unravelling molecular dynamics in living cells: Fluorescent protein biosensors for cell biology.","authors":"Colline Sanchez, Andrea Ramirez, Louis Hodgson","doi":"10.1111/jmi.13270","DOIUrl":"10.1111/jmi.13270","url":null,"abstract":"Genetically encoded, fluorescent protein (FP)-based Förster resonance energy transfer (FRET) biosensors are microscopy imaging tools tailored for the precise monitoring and detection of molecular dynamics within subcellular microenvironments. They are characterised by their ability to provide an outstanding combination of spatial and temporal resolutions in live-cell microscopy. In this review, we begin by tracing back on the historical development of genetically encoded FP labelling for detection in live cells, which lead us to the development of early biosensors and finally to the engineering of single-chain FRET-based biosensors that have become the state-of-the-art today. Ultimately, this review delves into the fundamental principles of FRET and the design strategies underpinning FRET-based biosensors, discusses their diverse applications and addresses the distinct challenges associated with their implementation. We place particular emphasis on single-chain FRET biosensors for the Rho family of guanosine triphosphate hydrolases (GTPases), pointing to their historical role in driving our understanding of the molecular dynamics of this important class of signalling proteins and revealing the intricate relationships and regulatory mechanisms that comprise Rho GTPase biology in living cells.","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11324865/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139735452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0