Journal of microscopy最新文献_第8页

Tales from the crick: The art of demo 克里克的故事：演示的艺术

IF 2 4区工程技术

Journal of microscopy Pub Date : 2024-04-21 DOI: 10.1111/jmi.13305

Matthew J. Renshaw, Camille Charoy

引用次数: 0

Modulated illumination microscopy: Application perspectives in nuclear nanostructure analysis 调制照明显微镜：核纳米结构分析的应用前景

IF 1.5 4区工程技术

Journal of microscopy Pub Date : 2024-04-15 DOI: 10.1111/jmi.13297

Christoph Cremer, Florian Schock, Antonio Virgilio Failla, Udo Birk

{"title":"Modulated illumination microscopy: Application perspectives in nuclear nanostructure analysis","authors":"Christoph Cremer, Florian Schock, Antonio Virgilio Failla, Udo Birk","doi":"10.1111/jmi.13297","DOIUrl":"10.1111/jmi.13297","url":null,"abstract":"The structure of the cell nucleus of higher organisms has become a major topic of advanced light microscopy. So far, a variety of methods have been applied, including confocal laser scanning fluorescence microscopy, 4Pi, STED and localisation microscopy approaches, as well as different types of patterned illumination microscopy, modulated either laterally (in the object plane) or axially (along the optical axis). Based on our experience, we discuss here some application perspectives of Modulated Illumination Microscopy (MIM) and its combination with single-molecule localisation microscopy (SMLM). For example, spatially modulated illumination microscopy/SMI (illumination modulation along the optical axis) has been used to determine the axial extension (size) of small, optically isolated fluorescent objects between ≤ 200 nm and ≥ 40 nm diameter with a precision down to the few nm range; it also allows the axial positioning of such structures down to the 1 nm scale; combined with laterally structured illumination/SIM, a 3D localisation precision of ≤1 nm is expected using fluorescence yields typical for SMLM applications. Together with the nanosizing capability of SMI, this can be used to analyse macromolecular nuclear complexes with a resolution approaching that of cryoelectron microscopy.","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":"296 2","pages":"121-128"},"PeriodicalIF":1.5,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jmi.13297","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140593055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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IF 2 4区工程技术

Journal of microscopy Pub Date : 2024-04-15 DOI: 10.1111/jmi.13198

引用次数: 0

Surface passivation and functionalisation for mass photometry 用于质量光度测量的表面钝化和功能化技术

IF 2 4区工程技术

Journal of microscopy Pub Date : 2024-04-12 DOI: 10.1111/jmi.13302

Jenny Sülzle, Laila Elfeky, Suliana Manley

引用次数: 0

Sulphate resistance of low-clinker engineered cementitious composites examined by MicroXRF imaging 通过 MicroXRF 成像检测低熟料工程水泥基复合材料的抗硫酸盐性能

IF 2 4区工程技术

Journal of microscopy Pub Date : 2024-04-10 DOI: 10.1111/jmi.13303

Connor Szeto, Kimberly E. Kurtis

{"title":"Sulphate resistance of low-clinker engineered cementitious composites examined by MicroXRF imaging","authors":"Connor Szeto, Kimberly E. Kurtis","doi":"10.1111/jmi.13303","DOIUrl":"https://doi.org/10.1111/jmi.13303","url":null,"abstract":"Engineered cementitious composites (ECC) are a class of high-performing fibre-reinforced cementitious materials recognised for their increased ductility and durability compared to conventional cement-based materials, owing to their autogenously controlled tight crack widths, even when subjected to high strains. To reduce ECC's environmental impact, this research examines the use of a low-clinker binder − limestone-calcined clay cement (LC3) − as an alternative to portland cement (PC), along with fly ash to further reduce the clinker proportion and the embodied CO2 of the formulations. In conventional concrete, LC3 hydrates to a denser microstructure resulting from the synergistic reaction between limestone and calcined clay. At the lower water contents typical of ECC and with the presence of fly ash, the influence of the binder composition on the microstructure is difficult to anticipate.To examine the influence of these compositional variables on microstructure, permeability and durability, the sulphate resistance of LC3-based ECC is explored. Specifically, the ECC-LC3 blends are designed with high clinker replacement rate of 75% by mass of binder and contain either conventional fly ash or reclaimed fly ash at 50% by mass of binder. Expansion of ECC-LC3 samples subjected to standard sodium sulphate test conditions was measured up to 12 months and the depth of penetration of sulphates into the ECC-LC3 of varying compositions was quantified using micro-X-Ray Fluorescence (microXRF) imaging and modelling. The expansion results show that the ECC-LC3 formulations performed better than the PC samples and can provide adequate resistance to external sulphate attack, even when reclaimed fly ashes are used in place of the conventional ash. In addition, the shallow penetration of sulphate into these cementitious composites demonstrates the low diffusion coefficients values that were determined using the quantitative data from MicroXRF imaging.","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":"294 2","pages":"239-250"},"PeriodicalIF":2.0,"publicationDate":"2024-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jmi.13303","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140556339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Introduction for special issue: 19th Euroseminar on Microscopy Applied to Building Materials (EMABM) 2024 特刊简介：第 19 届欧洲建筑材料应用显微镜研讨会 (EMABM) 2024

IF 2 4区工程技术

Journal of microscopy Pub Date : 2024-04-10 DOI: 10.1111/jmi.13299

Alexander Wetzel

引用次数: 0

Studying crystallisation processes using electron microscopy: The importance of sample preparation 使用电子显微镜研究结晶过程：样品制备的重要性

IF 1.5 4区工程技术

Journal of microscopy Pub Date : 2024-04-09 DOI: 10.1111/jmi.13300

Martha Ilett, Maryam Afzali, Bilal Abdulkarim, Zabeada Aslam, Stephanie Foster, Miguel Burgos-Ruiz, Yi-Yeoun Kim, Fiona C. Meldrum, Rik M. Drummond-Brydson

引用次数: 0

Characterisation of bond between cement paste and steel fibres with different surface roughness using SEM 利用扫描电子显微镜分析不同表面粗糙度的水泥浆与钢纤维之间的粘结特性

IF 2 4区工程技术

Journal of microscopy Pub Date : 2024-04-05 DOI: 10.1111/jmi.13298

Anna Antonova, Marika Eik, Jari Puttonen

引用次数: 0

Quantification of cell shape, intracellular flows and transport based on DIC object detection and tracking 基于 DIC 物体检测和跟踪的细胞形状、细胞内流动和运输定量分析

IF 1.5 4区工程技术

Journal of microscopy Pub Date : 2024-04-04 DOI: 10.1111/jmi.13295

Tanvi Kale, Dhruv Khatri, Jashaswi Basu, Shivani A. Yadav, Chaitanya A. Athale

引用次数: 0

Enhancing resolution and contrast in fibre bundle-based fluorescence microscopy using generative adversarial network 利用生成式对抗网络提高基于纤维束的荧光显微镜的分辨率和对比度。

IF 1.5 4区工程技术

Journal of microscopy Pub Date : 2024-04-02 DOI: 10.1111/jmi.13296

Amir Mohammad Ketabchi, Berna Morova, Yiğit Uysalli, Musa Aydin, Furkan Eren, Nima Bavili, Dariusz Pysz, Ryszard Buczynski, Alper Kiraz

{"title":"Enhancing resolution and contrast in fibre bundle-based fluorescence microscopy using generative adversarial network","authors":"Amir Mohammad Ketabchi, Berna Morova, Yiğit Uysalli, Musa Aydin, Furkan Eren, Nima Bavili, Dariusz Pysz, Ryszard Buczynski, Alper Kiraz","doi":"10.1111/jmi.13296","DOIUrl":"10.1111/jmi.13296","url":null,"abstract":"Fibre bundle (FB)-based endoscopes are indispensable in biology and medical science due to their minimally invasive nature. However, resolution and contrast for fluorescence imaging are limited due to characteristic features of the FBs, such as low numerical aperture (NA) and individual fibre core sizes. In this study, we improved the resolution and contrast of sample fluorescence images acquired using in-house fabricated high-NA FBs by utilising generative adversarial networks (GANs). In order to train our deep learning model, we built an FB-based multifocal structured illumination microscope (MSIM) based on a digital micromirror device (DMD) which improves the resolution and the contrast substantially compared to basic FB-based fluorescence microscopes. After network training, the GAN model, employing image-to-image translation techniques, effectively transformed wide-field images into high-resolution MSIM images without the need for any additional optical hardware. The results demonstrated that GAN-generated outputs significantly enhanced both contrast and resolution compared to the original wide-field images. These findings highlight the potential of GAN-based models trained using MSIM data to enhance resolution and contrast in wide-field imaging for fibre bundle-based fluorescence microscopy.Lay Description: Fibre bundle (FB) endoscopes are essential in biology and medicine but suffer from limited resolution and contrast for fluorescence imaging. Here we improved these limitations using high-NA FBs and generative adversarial networks (GANs). We trained a GAN model with data from an FB-based multifocal structured illumination microscope (MSIM) to enhance resolution and contrast without additional optical hardware. Results showed significant enhancement in contrast and resolution, showcasing the potential of GAN-based models for fibre bundle-based fluorescence microscopy.","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":"295 3","pages":"236-242"},"PeriodicalIF":1.5,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140335962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0