Journal of Microbial & Biochemical Technology最新文献_第9页

Looking for the Integrome, When Metabolomics Talk about the Interactome 寻找整合组，当代谢组学谈论相互作用组

Journal of Microbial & Biochemical Technology Pub Date : 2018-01-01 DOI: 10.4172/1948-5948.1000E134

B. Juan

引用次数: 2

Nutritional Values of Moringa oleifera , Total Protein, Amino Acid, Vitamins, Minerals, Carbohydrates, Total Fat and Crude Fiber, under the Semi-Arid Conditions of Sudan 苏丹半干旱条件下辣木的营养价值、总蛋白质、氨基酸、维生素、矿物质、碳水化合物、总脂肪和粗纤维

Journal of Microbial & Biochemical Technology Pub Date : 2018-01-01 DOI: 10.4172/1948-5948.1000396

R. Abbas, F. S. Elsharbasy, Abdalfatah Abdalla Fadlelmula

{"title":"Nutritional Values of Moringa oleifera , Total Protein, Amino Acid, Vitamins, Minerals, Carbohydrates, Total Fat and Crude Fiber, under the Semi-Arid Conditions of Sudan","authors":"R. Abbas, F. S. Elsharbasy, Abdalfatah Abdalla Fadlelmula","doi":"10.4172/1948-5948.1000396","DOIUrl":"https://doi.org/10.4172/1948-5948.1000396","url":null,"abstract":"Moringa oleifera plant is used in the semiarid, tropical, and subtropical areas as food and medicinal compounds. The leave extract of Moringa), was studied by evaluating total protein, amino acid, vitamins, minerals, total fat and crude fiber under the semi-arid conditions of Sudan. HPLC and amino acid analyzer, atomic absorption spectrophotometry, Soxlet extractor, Kjeldahl methods were used in this study. It was found that, carbohydrates 9.1 g, dietary fiber 2.1 g, fat 1.7 g, protein 8.1 g also rich in vitamins such as vitamin A 80 μg, thiamine (B1) 0.103 mg, Riboflavin (B2) 0.112 mg, Niacin (B3) 1.5 pantothenic acids (B5) 0.48 mg. vitamin B6 0.129 mg folic acid (B9) 41 μg Vitamin C 8.6 mg also rich in minerals such as Calcium 99.1 mg, Iron 1.3 mg, Magnesium 35.1 mg Mangenase 0.119 mg, Phosphorus 70.8 mg, Potassium 471 mg, Sodium 70 mg, Zinc 0.85 mg Also rich in essentials amino acids (ug/ml) Threonine 36.77, Valine 22.1, Methionine 2.13, Leucine 20.50, Isoleucine 31.8, Phenylalanine36.8, Histidine 30.88, Lycine 27.67, Argnine 21.45.","PeriodicalId":16453,"journal":{"name":"Journal of Microbial & Biochemical Technology","volume":"16 1","pages":"56-58"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80002135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 44

Study of Correlation between Milk Yield and Intestinal Microbes in Chinese Holstein Cows under the Same Diet 相同日粮条件下中国荷斯坦奶牛产奶量与肠道微生物的相关性研究

Journal of Microbial & Biochemical Technology Pub Date : 2018-01-01 DOI: 10.4172/1948-5948.1000403

W. Xiuying, W. Hongyu, Y. Zhenya, W. Guozhen, X. Xia

{"title":"Study of Correlation between Milk Yield and Intestinal Microbes in Chinese Holstein Cows under the Same Diet","authors":"W. Xiuying, W. Hongyu, Y. Zhenya, W. Guozhen, X. Xia","doi":"10.4172/1948-5948.1000403","DOIUrl":"https://doi.org/10.4172/1948-5948.1000403","url":null,"abstract":"The purpose of this study is to characterize and compare the gut microbes in six Chinese Holstein cows (CHC1, CHC2, CHC3, CHC4, CHC5, CHC6) under the same diet but different milk yields. High-throughput sequencing analysis was used to reveal and compare bacterial communities in faeces. The results showed 13 phyla in the six samples and the most dominant phylum was Firmicutes (approximately accounts for 71% OTUs), in which 18% OTUs were affiliated with Ruminococcaceae. Bacteroidetes was the subdominant phylum, which contributed to 17.5% of the total OTUs. While the majority of the bacterial genera found were unclassified, Solibacillus and Acinetbacter were the first and second predominant genera. Among the cases, the diversity and abundance of gut microbes in the Chinese Holstein cows have no significant difference under the same diet, which suggests that the relationship between milk yield and gut microbes is stable. The factors related to milk yield were calculated with spearman's rank (p<0.05), the results showed that milk yield is significantly associated with age (P=0.029) and body length (P=0.021) of Chinese Holstein cows. By LEfSe analysis (LDA threshold of 2), screening out microorganisms with significant differences in the gut of different milking Chinese Holstein cows. For the reluts, Lachnospiraceae, Mollicutes, Tenericutes Mollicutes, Tenericutes apparently enriched in HMY, but only Fibrobacteraceae apparently enriched in LMY. This indicates microbes play an important role in the milk production of dairy Chinese Holstein cows.","PeriodicalId":16453,"journal":{"name":"Journal of Microbial & Biochemical Technology","volume":"39 1","pages":"112-118"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81343947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

BETA – Endorphins – A Novel Natural Holistic Healer β -内啡肽-一种新颖的天然整体治疗剂

Journal of Microbial & Biochemical Technology Pub Date : 2018-01-01 DOI: 10.4172/1948-5948.1000391

Shrihari Tg

引用次数: 11

Upgrading of Tomato (Solanum lycopersicum) Agroindustrial Wastes 番茄(Solanum lycopersicum)农工废弃物的资源化利用

Journal of Microbial & Biochemical Technology Pub Date : 2018-01-01 DOI: 10.4172/1948-5948.1000394

K. Zh, Nour Sh El Gendy, A. Ha, Shaltout Tht, Hussein Mmd

引用次数: 4

Assessing Various Types of Fungal Strains to Convert Soybean Processing Industry Wastewater into Protein-Rich Animal Feed 大豆加工废水转化为富含蛋白质饲料的几种真菌菌株的鉴定

Journal of Microbial & Biochemical Technology Pub Date : 2018-01-01 DOI: 10.4172/1948-5948.1000390

Jacob D. Zahler, B. Karki, I. C. Isaac, W. Gibbons

引用次数: 2

Comparison of GeneXpert against Light-Emitting Diode Fluorescent Microscopy for the Diagnosis of Pulmonary Tuberculosis in Addis Ababa, Ethiopia GeneXpert与发光二极管荧光显微镜在埃塞俄比亚亚的斯亚贝巴诊断肺结核的比较

Journal of Microbial & Biochemical Technology Pub Date : 2018-01-01 DOI: 10.4172/1948-5948.1000388

B. D. Taddese, D. Desalegn, A. S. Misganaw, K. T. Kitila, Hanna Mekonnen Balcha, C. Gebeyehu, Tinsae K Hailu, Abraham Tesfaye Bika

{"title":"Comparison of GeneXpert against Light-Emitting Diode Fluorescent Microscopy for the Diagnosis of Pulmonary Tuberculosis in Addis Ababa, Ethiopia","authors":"B. D. Taddese, D. Desalegn, A. S. Misganaw, K. T. Kitila, Hanna Mekonnen Balcha, C. Gebeyehu, Tinsae K Hailu, Abraham Tesfaye Bika","doi":"10.4172/1948-5948.1000388","DOIUrl":"https://doi.org/10.4172/1948-5948.1000388","url":null,"abstract":"Background: Tuberculosis remains a global health problem despite availability of effective tools. Globally, there were 40% of the 10+ million patients with TB did not get diagnosed or notified. The study was aimed to compare the diagnostic performances of iLED-FM and GeneXpert test for the diagnosis of PTB in Addis Ababa, Ethiopia. Methods: Facility-based cross-sectional study was conducted on a total of 286 sputum samples collected from health centers and hospitals clients with presumptive TB from December 2016 to March 2017. Kappa value, Sensitivity, Specificity, Positive Predictive Value (PPV) and Negative Predictive Value (NPV) of iLED-FM and GeneXpert were calculated against the gold standard. Results: The sensitivity, specificity, PPV and NPV of iLED-FM was 80.15%, 95.48%, 93.75% and 85.06%, for GeneXpert 88.55%, 92.90%, 91.34% and 90.57%, respectively. Kappa value of iLED-FM was 0.765 and 0.817 for GeneXpert. Out of 55 HIV positive presumptive TB patients enrolled in the study, 19 (34.55%) were sputum smear positive by iLED-FM. However, 24 (43.64%) TB cases were detected by GeneXpert. Conclusion: The sensitivity of Xpert MTB/RIF assay was better than iLED-FM in the diagnosis of PTB. It should be implemented as primary diagnostic test in areas where overlapping synergy of TB and HIV/AIDS is high.","PeriodicalId":16453,"journal":{"name":"Journal of Microbial & Biochemical Technology","volume":"115 1","pages":"12-15"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77904887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Effect of Storage Time on Nutrient, Biodeteriorating Fungi and Aflatoxin Contents of Bitter Leaf ( Vernonia amygadalina ) and JUTE ( Corchorus olitorius ) 贮藏时间对苦叶和黄麻养分、生物腐坏真菌和黄曲霉毒素含量的影响

Journal of Microbial & Biochemical Technology Pub Date : 2018-01-01 DOI: 10.4172/1948-5948.1000395

G. JonathanSegun, G. OnileOlanrewaju, D. AsemoloyeMichael, O. OmotayoOmolola

{"title":"Effect of Storage Time on Nutrient, Biodeteriorating Fungi and Aflatoxin Contents of Bitter Leaf ( Vernonia amygadalina ) and JUTE ( Corchorus olitorius )","authors":"G. JonathanSegun, G. OnileOlanrewaju, D. AsemoloyeMichael, O. OmotayoOmolola","doi":"10.4172/1948-5948.1000395","DOIUrl":"https://doi.org/10.4172/1948-5948.1000395","url":null,"abstract":"Unhygienic handlings of several African food products during storage have been reported to promote fungal biodeterioration and subsequent aflatoxin contamination. Therefore, this study was aimed to investigate the effect of storage time on nutrient, commonly associated bio-deteriorating fungi and aflatoxin contents of selected vegetables; Bitter leaf (Vernonia amygdalina) and Jute (Corchorus olitorius) in Nigeria. Fresh and stored vegetable samples (24) were collected from different markets in Ibadan, Nigeria, they were analyzed for proximate (% Moisture, Carbohydrate, Crude Protein, Fat and Fibre), nutrient (Potassium, Calcium, Phosphorus, Sodium Iron, Zinc and Magnesium), fungal and aflatoxin contents (AFB1, AFB2, AFG1 and AFG2). Most dominantly associated fungal strains were identified as Aspergillus niger, Aspergillus flavus, Aspergillus terreus, Aspergillus tamari, Aspergillus parasiticus, Aspergillus fumigatus, Penicillium chrysogenum and Rhizopus nigricans. The fungal incidence was more pronounced in the stored samples as compared to the fresh samples. The storage time also significantly affected the nutrient and aflatoxin contents of the vegetables; most nutrients decreased with increasing storage period while the aflatoxin contents increased. The study affirms that the storage time has effect on the nutrient, fungal contamination and consequently on the associated aflatoxin, although the detected aflatoxin levels were below the tolerance limit but these may increase with the storage time. It is therefore advisable to take hygienic measures during storage period and also, prolong storage should be discouraged.","PeriodicalId":16453,"journal":{"name":"Journal of Microbial & Biochemical Technology","volume":"20 1","pages":"49-55"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84917086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

RNA Extraction from the Yeast Candida parapsilosis Sensu Stricto Using Two Commercial Methods Based on Purification by Silica Columns 以二氧化硅柱纯化为基础的两种商业方法提取严格假丝酵母RNA

Journal of Microbial & Biochemical Technology Pub Date : 2018-01-01 DOI: 10.4172/1948-5948.1000401

Rodríguez Ml, Rosa Ac, Jewtuchowicz Vm

{"title":"RNA Extraction from the Yeast Candida parapsilosis Sensu Stricto Using Two Commercial Methods Based on Purification by Silica Columns","authors":"Rodríguez Ml, Rosa Ac, Jewtuchowicz Vm","doi":"10.4172/1948-5948.1000401","DOIUrl":"https://doi.org/10.4172/1948-5948.1000401","url":null,"abstract":"Good quality RNA needs to be obtained in order to study gene expression. Different RNA extraction methods have been described, but RNA quality and yield may vary among the different techniques and biological study species. To date, there is no standardized method for extraction and purification of RNA from Candida genus yeasts. The few available papers on the subject apply mainly to filamentous fungi and have produced poor results for extraction techniques based on manual or in-house IVD methods. The aim of this study was therefore to compare two commercial RNA extraction and purification systems using silica columns (Qiagen and Zymo Research) with Candida parapsilosis sensu stricto as model organism. This yeast has been identified in recent papers as the second most frequently isolated Candida species in the oral cavity. In the past decade, it has been the object of increasing medical interest because it is one of the main causes of candidemia in both adults and preterm neonates. In view of this background, we consider the study of Candida parapsilosis sensu stricto transcriptome and its variations according to environmental changes to be a priority. In this experimental study, 19 fungal isolates were processed using Qiagen and 17 isolates using Zymo Research. The results suggest that Qiagen lysis buffer RLT is essential for obtaining better quality RNA product.","PeriodicalId":16453,"journal":{"name":"Journal of Microbial & Biochemical Technology","volume":"35 1","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74440868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial Fuel Cell: An Efficient Method to Utilize Prokaryotic Potential to Engender Reliable Energy 微生物燃料电池:利用原核潜能产生可靠能源的有效方法

Journal of Microbial & Biochemical Technology Pub Date : 2018-01-01 DOI: 10.4172/1948-5948.1000398

Ankur B, Shipra S

{"title":"Microbial Fuel Cell: An Efficient Method to Utilize Prokaryotic Potential to Engender Reliable Energy","authors":"Ankur B, Shipra S","doi":"10.4172/1948-5948.1000398","DOIUrl":"https://doi.org/10.4172/1948-5948.1000398","url":null,"abstract":"A MFC (Microbial Fuel Cell), a microbial power module is a novel and exquisite advancement serving reasonable and green bio-vitality change innovation in the midst of microbial assimilation of regular acids and sugars. In commenced work twofold microbial operated energy chambers were constructed with diverged nature of microorganisms. In MFC-R1, E. coli (Escherichia coli) was utilized in anode slot while in MFC-R2, the sludge samples, anaerobically activated were exploited as anode and aerobically activated in cathode. The extreme yield of voltage was 150 mV in MFC-R1 after adding glucose and 400 mV in MFC-R2. In absence of appropriate air circulation in MFC-R1 voltage yield was diminished to 110 mV, while after proper aeration, the voltage yield has increased up-to 140 mV. In MFC-R2 voltage generation was reduced (250 mV) in deficiency of airing and increased up-to 400 mV after providing proper aeration. In MFC-R2, the higher voltage sustained for an extensive period (for 4 days) while in MFC-R1 voltage output declined after Day 1. This may be happened because in MFC-R2, additional substrates (the sludge samples rich in biomass/nutrients) were present and diverse sorts of microbes may be grown under wild conditions (belonging to different genus/species/strains) to utilize that substrate. Subsequently, the MFCs are being produced for both waste management besides bio-electricity generation and what's more, the thought would be fiscally functional and cooperative ecologically.","PeriodicalId":16453,"journal":{"name":"Journal of Microbial & Biochemical Technology","volume":"21 1","pages":"69-75"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86097200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3