Journal of Microbial & Biochemical Technology最新文献_第9页

Biofilm Production by Multi Drug Resistant Bacterial Pathogens IsolatedFrom Patients in Intensive Care Units in Egyptian Hospitals 从埃及医院重症监护病房患者中分离的多重耐药细菌病原体的生物膜生产

Journal of Microbial & Biochemical Technology Pub Date : 2017-07-18 DOI: 10.4172/1948-5948.1000359

Elhabibi T, R. S

{"title":"Biofilm Production by Multi Drug Resistant Bacterial Pathogens IsolatedFrom Patients in Intensive Care Units in Egyptian Hospitals","authors":"Elhabibi T, R. S","doi":"10.4172/1948-5948.1000359","DOIUrl":"https://doi.org/10.4172/1948-5948.1000359","url":null,"abstract":"Antibiotic resistance among Multi drug resistant (MDR) Gram negative bacteria causing hospital acquired infections poses a great threat in ICU patients. The treatment of such infections has become increasingly problematic, due to their intrinsic and/or acquired resistance to variable classes of antibiotics. Moreover, the demonstrated ability of these bacteria to grow as biofilm is believed to have a major role in their ability to resist various antibiotics. The aim of this study is to evaluate the role of the selected genes in biofilm formation in 3 significant MDR bacterial isolates (Acinetobacter baumannii and Pseudomonas aeruginosa and Stenotrophomonas maltophilia). In this study a total of 625 non replicated Gram negative non-fermenter bacterial isolates were isolated from different clinical specimens from intensive care units from hospitals in Egypt. These bacterial isolates were identified biochemically, API20E and genetically. The antibiogram of all isolates was determined and revealed that all isolates were MDR and colistin was the most potent antibiotic against all A. baumannii and P. aeruginosa isolates. While trimethoprim/sulfamethoxazole combination was the most potent against all S. maltophilia isolates. Detection of biofilm formation of isolates was done by Tube method. While, Quantification of biofilm formation was done by the microtiter plate method using crystal violet (CV) assay. Screening for some selected genes responsible for biofilm formation was done by PCR as bap gene which is responsible for biofilm formation in A. baumannii, rhlI gene in P. aeruginosa strains and rmlA, spgM, rpfF genes in S. maltophilia. The results revealed the presence of these genes in both strong and weak biofilm producer isolates. These final results showed the significance of these genes in biofilm formation.","PeriodicalId":16453,"journal":{"name":"Journal of Microbial & Biochemical Technology","volume":"19 1","pages":"151-158"},"PeriodicalIF":0.0,"publicationDate":"2017-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83405875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Commentary on Appropriate Wastewater Treatment System for a NaturalRubber Processing Factory 某天然橡胶加工厂污水处理系统的适宜性探讨

Journal of Microbial & Biochemical Technology Pub Date : 2017-07-18 DOI: 10.4172/1948-5948.1000360

T. Daisuke

引用次数: 5

Diverse Pattern of Cytokine Production, their Functional Association andmRNA Expression in Tuberculosis Patients with Diabetes Mellitus and theirHousehold Contacts 结核糖尿病患者及其家庭接触者细胞因子产生模式、功能关联及mrna表达

Journal of Microbial & Biochemical Technology Pub Date : 2017-07-03 DOI: 10.4172/1948-5948.1000358

P. Meenakshi, Sivangala Ramya, A. Madhavilatha, J. Lavanya, G. Sumanlatha

{"title":"Diverse Pattern of Cytokine Production, their Functional Association andmRNA Expression in Tuberculosis Patients with Diabetes Mellitus and theirHousehold Contacts","authors":"P. Meenakshi, Sivangala Ramya, A. Madhavilatha, J. Lavanya, G. Sumanlatha","doi":"10.4172/1948-5948.1000358","DOIUrl":"https://doi.org/10.4172/1948-5948.1000358","url":null,"abstract":"Cytokine genes are associated with their altered production and play a significant role in tuberculosis patients with diabetes mellitus and their household contacts. We aimed to study the influence of IL-1β and IL-6 gene polymorphisms on cytokine gene production and to evaluate their mRNA expression in M.tbAg85A stimulated culture supernatants of tuberculosis patients with diabetes mellitus, pulmonary tuberculosis patients, household contacts and diabetes individuals. Enzyme linked immunosorbent assay was used to estimate cytokine production in the culture supernatants and TRIZOL method for mRNA expression. Cytokine production was low in all the subjects when compared to the healthy controls and IL-6 levels increased with treatment. The CC genotype of IL-6 -174 G/C was associated with its low production in household contacts of tuberculosis patients with diabetes mellitus and pulmonary tuberculosis patients. The mRNA expression of IL-1β increased and that of IL-6 decreased in tuberculosis patients with diabetes mellitus, while that of IL-6 decreased with treatment in pulmonary tuberculosis patients; however, there was no significant difference in the household contacts. The cytokine levels were almost similar in patients and their household contacts at the time of diagnosis suggesting that even the household contacts might be infected thus aiding in protecting the high-risk individuals by their prior identification.","PeriodicalId":16453,"journal":{"name":"Journal of Microbial & Biochemical Technology","volume":"109 1","pages":"143-150"},"PeriodicalIF":0.0,"publicationDate":"2017-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75204477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Recent Technology of Clinical Microbiology; Whole Genome Sequencing(WGS) and Matrix-Assisted, Laser Desorption Ionization-Time of FlightMass Spectrometry (MALDI-TOF MS) 临床微生物学新技术;全基因组测序(WGS)和基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)

Journal of Microbial & Biochemical Technology Pub Date : 2017-06-26 DOI: 10.4172/1948-5948.1000357

Y. Jeongsook

{"title":"Recent Technology of Clinical Microbiology; Whole Genome Sequencing(WGS) and Matrix-Assisted, Laser Desorption Ionization-Time of FlightMass Spectrometry (MALDI-TOF MS)","authors":"Y. Jeongsook","doi":"10.4172/1948-5948.1000357","DOIUrl":"https://doi.org/10.4172/1948-5948.1000357","url":null,"abstract":"The hottest issue in the field of clinical microbiology in latest years is the application of whole genome sequencing (WGS) to the clinical diagnostics and therapeutics through the methodology of Next generation sequencing. The application of WGS includes species identification, epidemiological study and study of antimicrobial resistance and so on. The single nucleotide polymorphism (SNP) based analysis increases the accuracy and sensitivity of species identification by marked discriminatory power, and the ability of tracking of transmission process enables infection control including outbreaks. We can clarify the antimicrobial resistance mechanism of extended spectrum betalactamases or carbapenemases and elucidate the mechanism of horizontal transfer of mobile genomic islands. In case of setting up a new PCR method, the target and the primer could be chosen by using WGS databases. The MALDI-TOF method is used in nearly all laboratories, identifying bacterial, fungal and mycobacterial species. Direct identification in blood culture bottle is possible in bacteremia patients, and Beta-lactamases or Carbapenemases can be detected. In addition, it can be applied in Shiga toxin E. coli, Salmonella serotypes or C. difficile ribotypes.","PeriodicalId":16453,"journal":{"name":"Journal of Microbial & Biochemical Technology","volume":"9 1","pages":"138-142"},"PeriodicalIF":0.0,"publicationDate":"2017-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81675672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Effect of Antibiotic Combinations on the Sensitivity of CarbapenemResistant Acinetobacter baumannii Strains 抗生素组合对耐碳青霉烯鲍曼不动杆菌敏感性的影响

Journal of Microbial & Biochemical Technology Pub Date : 2017-06-15 DOI: 10.4172/1948-5948.1000356

Elhabibi T, R. S

{"title":"Effect of Antibiotic Combinations on the Sensitivity of CarbapenemResistant Acinetobacter baumannii Strains","authors":"Elhabibi T, R. S","doi":"10.4172/1948-5948.1000356","DOIUrl":"https://doi.org/10.4172/1948-5948.1000356","url":null,"abstract":"Acinetobacter baumannii is one of the predominant pathogens involved in hospital acquired infections worldwide. Despite intensive surveillance and preventive measures, these infections are major cause of high mortality rates. In this study a total of 375 A. baumannii isolates were isolated from a variety of clinical collected from 5 different hospitals in Great Cairo. These isolates were identified as A. baumannii biochemically, by API20E system and genetically by detection of 16S rRNA gene. All isolates showed positive results and confirmed for the presence of the suspected gene by PCR. The antibiotic susceptibility patterns for the isolated A. baumannii were also evaluated. The detection of the presence of OXA-type (OXA 23, 24, 51 and 58) carbapenemases-encoding genes which are responsible for carbapenem resistance was also performed. I observed the presence of OXA 23 gene in 84% of the isolates. While 35.2% were positive to OXA 24 gene and 87.2% were positive to OXA 51 gene. No isolates showed positive results for the presence of OXA 51 gene. They were also evaluated for the effectiveness of drug combination of carbapenems and colistin against 30 isolates. Regarding imipenem and colistin combination, 13.3% of the strains showed synergy, while 86.7% showed additive results. For the combination of meropenem and colistin, 66.7% of the strains showed synergy, while 33.3% showed additive results. The antibacterial effect of both combinations on A. baumannii showed commonly synergistic or additive results. To a significant extent meropenem and colistin showed a superior synergy compared to imipenem and colistin.","PeriodicalId":16453,"journal":{"name":"Journal of Microbial & Biochemical Technology","volume":"31 1","pages":"132-137"},"PeriodicalIF":0.0,"publicationDate":"2017-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90498797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Cucurbit Extracts Augment Biofilm Formation by Probiotic Lactobacilli: AnIn Vitro Study 瓜类提取物促进益生菌乳酸菌生物膜形成的体外研究

Journal of Microbial & Biochemical Technology Pub Date : 2017-06-10 DOI: 10.4172/1948-5948.1000354

A. Irfan, M. AsadKhan, M. SyedHassan, Md. Zafaryab, P. Ahmad, M. MoshahidARizvi

{"title":"Cucurbit Extracts Augment Biofilm Formation by Probiotic Lactobacilli: AnIn Vitro Study","authors":"A. Irfan, M. AsadKhan, M. SyedHassan, Md. Zafaryab, P. Ahmad, M. MoshahidARizvi","doi":"10.4172/1948-5948.1000354","DOIUrl":"https://doi.org/10.4172/1948-5948.1000354","url":null,"abstract":"Gastrointestinal tract predominantly harbor trillions of microorganisms including probiotics which maintain enteric microbial homeostasis. Some dietary cucurbits were investigated for their effects on biofilm formation by Lactobacillus rhamnosus (L. rhamnosus), Lactobacillus plantarum (L. plantarum), Lactobacillus acidophilus (L. acidophilus), Escherichia coli (E. coli) and Salmonella enterica typhimurium (S. enterica typhi). Aqueous and methanol extracts of Lagenaria siceraria (Ls), Luffa cylindrica (Lc) and Cucurbita maxima (Cm) were prepared and evaluated their effective concentrations on these bacterial strains. Effective concentrations for methanol and aqueous extracts were found 93.60 μg/mL-115.40 μg/mL and 103.67 μg/mL-121.00 μg/mL, respectively. For the both types of extracts toxicity was determined up to 1 mg/mL concentration and found no microbicidal effects on probiotic strains. However, marginal inhibition on the growth of biofilms of pathogenic bacteria was observed. Extracts were found to support growth of biofilms of probiotics. Ls and Lc exhibited marginal inhibition on biofilm formation by E. coli and S. enterica typhi. Therefore, on the basis of our results it may be said that Lagenaria siceraria (bottle guard) and Luffa cylindrica (sponge guard) are safe, non-toxic and may be recommended as nutraceutical.","PeriodicalId":16453,"journal":{"name":"Journal of Microbial & Biochemical Technology","volume":"29 1","pages":"122-125"},"PeriodicalIF":0.0,"publicationDate":"2017-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83530120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Up-regulation of Lamin A/C Expression in Epstein-Barr Virus Immortalized B Cells and Burkitt Lymphoma Cell Lines of Activated B Cell Phenotype eb病毒永生化B细胞和活化B细胞表型Burkitt淋巴瘤细胞株中Lamin A/C表达的上调

Journal of Microbial & Biochemical Technology Pub Date : 2017-05-22 DOI: 10.4172/1948-5948.1000349

F. Bánáti, Anita Koroknai, K. Szenthe, Tamás Tereh, Anita O. Hidasi, B. Bánkúti, K. Buzas, Frederic Lemnitzer, Z. Ruzsics, S. Szathmary, H. Wolf, D. Salamon, J. Minárovits, H. Niller

{"title":"Up-regulation of Lamin A/C Expression in Epstein-Barr Virus Immortalized B Cells and Burkitt Lymphoma Cell Lines of Activated B Cell Phenotype","authors":"F. Bánáti, Anita Koroknai, K. Szenthe, Tamás Tereh, Anita O. Hidasi, B. Bánkúti, K. Buzas, Frederic Lemnitzer, Z. Ruzsics, S. Szathmary, H. Wolf, D. Salamon, J. Minárovits, H. Niller","doi":"10.4172/1948-5948.1000349","DOIUrl":"https://doi.org/10.4172/1948-5948.1000349","url":null,"abstract":"Lamin A, B and C, the nuclear intermediate-filament proteins, play a role in epigenetic regulation. Lamin B could be detected in all nucleated cells studied, whereas the lamin A and lamin C isoforms (lamin A/C) encoded by the LMNA gene are co-expressed in most somatic cell types except mature B lymphocytes. Since Epstein-Barr virus (EBV), a human gammaherpesvirus, is associated with tumorigenic processes and is known to alter the epigenotype of its host cells, we studied the expression of the LMNA gene and its epigenetic marks in EBV-carrying human lymphoid cell lines. We observed a high lamin A/C mRNA expression in EBV-immortalized B lymphoblastoid cell lines (LCLs) and in a subset of Burkitt lymphoma (BL) lines characterized by an activated B cell phenotype and a unique latent EBV gene expression pattern (latency III). In these cells the first exon of LMNA was hypomethylated and associated with activating histone marks. In contrast, we observed a low level of lamin A/C mRNA expression in EBV negative BL lines and BL lines with a restricted expression of latent EBV products (latency I). Low LMNA promoter activity was associated with hypermethylation of the LMNA first exon. These data suggest a role for EBV latency products in switching on or upregulating the LMNA promoter (LMNAp) in EBV-infected activated B cells in vitro. Lamin A/C may contribute to the establishment of the activated B cell phenotype. Our data also imply a role of LMNA first exon methylation in the silencing of LMNAp.","PeriodicalId":16453,"journal":{"name":"Journal of Microbial & Biochemical Technology","volume":"148 1","pages":"087-094"},"PeriodicalIF":0.0,"publicationDate":"2017-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76761190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Detection of Aflatoxins, Mutagens and Carcinogens in Black, White andGreen Peppers (Piper Nigrum L.) 黑椒、白椒和青椒中黄曲霉毒素、诱变剂和致癌物的检测

Journal of Microbial & Biochemical Technology Pub Date : 2017-05-22 DOI: 10.4172/1948-5948.1000350

Jesús Garduño-Garcia, M. Carvajal-Moreno, F. Rojo-Callejas, S. Ruiz-Velasco

{"title":"Detection of Aflatoxins, Mutagens and Carcinogens in Black, White andGreen Peppers (Piper Nigrum L.)","authors":"Jesús Garduño-Garcia, M. Carvajal-Moreno, F. Rojo-Callejas, S. Ruiz-Velasco","doi":"10.4172/1948-5948.1000350","DOIUrl":"https://doi.org/10.4172/1948-5948.1000350","url":null,"abstract":"Aflatoxins, bis-dihydro-furancoumarins, are secondary metabolites that are produced by molds of Aspergillus sp. with adverse effects in humans and animals. The International Agency for Research on Cancer classifies aflatoxins in Group 1 of proven human carcinogens. Thus, aflatoxins in foods are highly regulated throughout the world. The purpose of this research was to identify and quantify aflatoxins in 54 pepper samples (19 black, 19 white and 16 green peppers) from markets in Egypt, India, Turkey and the 16 boroughs of Mexico City, as well as to validate the experimental method used. All samples were contaminated with at least one aflatoxin: 95% (51/54) were contaminated with aflatoxin B1 (0.1 to 218 μg kg-1); 80% (43/54) with aflatoxin B2 (0.4 to 382 μg kg-1); 67% (36/54) with aflatoxin G1 (0.4 to 612 μg kg-1); and 93% (50/54) with aflatoxin G2 (1.37 to 494 μg kg-1). Only 9.26% of the samples (5/54) were under the Mexican legislation limit, whereas all foreign samples surpassed the limits established for their respective countries. Although the aflatoxin concentrations in peppers are high, their ingestion is minimal because peppers are used in only small quantities as a flavor-enhancing product. Therefore, the contribution of aflatoxins from a pepper to an organism is relatively low in comparison to other agricultural products, such as maize, pistachio, peanuts and dairy products. Green pepper was the most contaminated with aflatoxins, white pepper was the least contaminated and black pepper had an intermediate level of contamination. This study describes a detailed analysis of aflatoxin contamination in pepper in three different ripening stages: green, black and white. The lack of normativity in countries on this subject prevents the reduction of AF concentrations in the diet","PeriodicalId":16453,"journal":{"name":"Journal of Microbial & Biochemical Technology","volume":"1 1","pages":"095-104"},"PeriodicalIF":0.0,"publicationDate":"2017-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75639598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Production and Characterization of Polyhydroxybutryrate Biopolymer from Azohydromonas australica Using Sucrose as a Sole Carbon Source 以蔗糖为唯一碳源的澳大利亚偶氮单胞菌制备聚羟基丁酸生物聚合物及其特性研究

Journal of Microbial & Biochemical Technology Pub Date : 2017-05-22 DOI: 10.4172/1948-5948.1000348

Salman Ahmad, Abuzer Amir, Md. Zafaryab, K. Osama, Soban Ahmad Faridi, M. H. Siddiqui, M. MoshahidARizvi, Md. Asad Khan

{"title":"Production and Characterization of Polyhydroxybutryrate Biopolymer from Azohydromonas australica Using Sucrose as a Sole Carbon Source","authors":"Salman Ahmad, Abuzer Amir, Md. Zafaryab, K. Osama, Soban Ahmad Faridi, M. H. Siddiqui, M. MoshahidARizvi, Md. Asad Khan","doi":"10.4172/1948-5948.1000348","DOIUrl":"https://doi.org/10.4172/1948-5948.1000348","url":null,"abstract":"A bacterial strain Azohydromonas australica DSM 1124 has been chosen, which is accumulated intracellular poly- β-hydroxybutyrate particles, each method which has been merit and demerit for employed in PHB extraction. Our study, we have selected chloroform-sodium hypochlorite method. It is one of the methods for extracting PHB forms Azohydromonas australica DSM 1124. Polyhydroxybutyrate is a biodegradable and biocompatible thermoplastic with many interesting applications in medicine, food packaging and tissue engineering materials. That does not produce any toxins or residues in the environment like petroleum based plastics. The present study was emphasized on enhanced production of PHB by Azohydromonas australica using sucrose as a sole carbon source and estimation of biomass and sucrose content in the media.The batch kinetics analysis of Azohydromonas australica was done an interval of 3 h. Batch cultivation with optimized media recipe in a 7 L bioreactor exhibited a maximum biomass 1.71 g/L and PHB concentration 2.67 g/L for A. australica. The characterization of PHB was done by growth kinetics studies, UV-Spectrophotometer and Fourier transforms infrared spectroscopy (FTIR).","PeriodicalId":16453,"journal":{"name":"Journal of Microbial & Biochemical Technology","volume":"2 1","pages":"082-086"},"PeriodicalIF":0.0,"publicationDate":"2017-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75246435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Forkhead Box Protein O1 is Linked to Anti-Inflammatory Probiotic BacteriaActing through Nuclear Factor-ÃÂºB Pathway Forkhead Box蛋白O1通过核因子-ÃÂºB途径与抗炎益生菌相关

Journal of Microbial & Biochemical Technology Pub Date : 2017-05-17 DOI: 10.4172/1948-5948.1000347

A. P. Mulet, Karen Perelmuter, M. Bollati-Fogolín, M. Crispo, Gianfranco Grompone

{"title":"Forkhead Box Protein O1 is Linked to Anti-Inflammatory Probiotic BacteriaActing through Nuclear Factor-ÃÂºB Pathway","authors":"A. P. Mulet, Karen Perelmuter, M. Bollati-Fogolín, M. Crispo, Gianfranco Grompone","doi":"10.4172/1948-5948.1000347","DOIUrl":"https://doi.org/10.4172/1948-5948.1000347","url":null,"abstract":"Probiotics are widely used to promote health benefits around the world. Nevertheless, the mechanisms whereby probiotics exert its beneficial effect on the host are not well elucidated yet. In an attempt to obtain relevant insights on probiotics mechanisms of action, we studied the probiotic response via Nuclear factor-κB (NF-κB) and Forkhead box protein O1 (FoxO1), two transcription factors that were previously related with probiotic effects. We performed in vitro analysis to activate these transcription factors with Tumor Necrosis Factor alpha (TNFα) and Hydrogen Peroxide (H2O2) stimuli using a set of probiotic strains co-cultured with HT-29 cells. We found three strains, LrBPL8, LcA1 and LaBPL71 capable to reducing the NF-κB activation pathway in an inflammatory context. We also found that LcA1 reduced FoxO1 activation while another strain, IPM C+, increased it after the hydrogen peroxide treatment under the same conditions. Moreover, we described a complex relationship between FoxO1 downstream gene expression and these anti-inflammatory strains. Our results show that more than one pathway could be targeting NF-κB modulation, indicating the complexity of the probiotics’ mechanisms of action. The in vitro data presented here may help to design multi-strain probiotics mix that take advantage of the complementary and synergistic effects that they may induce in the host.","PeriodicalId":16453,"journal":{"name":"Journal of Microbial & Biochemical Technology","volume":"12 1","pages":"74-81"},"PeriodicalIF":0.0,"publicationDate":"2017-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76343860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1