Journal of Muscle Research and Cell Motility最新文献_第7页

Cryo-electron tomography of intact cardiac muscle reveals myosin binding protein-C linking myosin and actin filaments. 完整心肌的冷冻电子断层扫描显示肌球蛋白结合蛋白-C连接肌球蛋白和肌动蛋白丝。

IF 2.7 3区生物学

Journal of Muscle Research and Cell Motility Pub Date : 2023-09-01 Epub Date: 2023-04-28 DOI: 10.1007/s10974-023-09647-3

Xinrui Huang, Iratxe Torre, Michele Chiappi, Zhan Yin, Anupama Vydyanath, Shuangyi Cao, Oliver Raschdorf, Morgan Beeby, Bonnie Quigley, Pieter P de Tombe, Jun Liu, Edward P Morris, Pradeep K Luther

{"title":"Cryo-electron tomography of intact cardiac muscle reveals myosin binding protein-C linking myosin and actin filaments.","authors":"Xinrui Huang, Iratxe Torre, Michele Chiappi, Zhan Yin, Anupama Vydyanath, Shuangyi Cao, Oliver Raschdorf, Morgan Beeby, Bonnie Quigley, Pieter P de Tombe, Jun Liu, Edward P Morris, Pradeep K Luther","doi":"10.1007/s10974-023-09647-3","DOIUrl":"10.1007/s10974-023-09647-3","url":null,"abstract":"Myosin binding protein C (MyBP-C) is an accessory protein of the thick filament in vertebrate cardiac muscle arranged over 9 stripes of intervals of 430 Å in each half of the A-band in the region called the C-zone. Mutations in cardiac MyBP-C are a leading cause of hypertrophic cardiomyopathy the mechanism of which is unknown. It is a rod-shaped protein composed of 10 or 11 immunoglobulin- or fibronectin-like domains labelled C0 to C10 which binds to the thick filament via its C-terminal region. MyBP-C regulates contraction in a phosphorylation dependent fashion that may be through binding of its N-terminal domains with myosin or actin. Understanding the 3D organisation of MyBP-C in the sarcomere environment may provide new light on its function. We report here the fine structure of MyBP-C in relaxed rat cardiac muscle by cryo-electron tomography and subtomogram averaging of refrozen Tokuyasu cryosections. We find that on average MyBP-C connects via its distal end to actin across a disc perpendicular to the thick filament. The path of MyBP-C suggests that the central domains may interact with myosin heads. Surprisingly MyBP-C at Stripe 4 is different; it has weaker density than the other stripes which could result from a mainly axial or wavy path. Given that the same feature at Stripe 4 can also be found in several mammalian cardiac muscles and in some skeletal muscles, our finding may have broader implication and significance. In the D-zone, we show the first demonstration of myosin crowns arranged on a uniform 143 Å repeat.","PeriodicalId":16422,"journal":{"name":"Journal of Muscle Research and Cell Motility","volume":" ","pages":"165-178"},"PeriodicalIF":2.7,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10542292/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9350272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

John squire and endothelial glycocalyx structure: an unfinished story. 约翰·斯奎尔和内皮糖盏结构：一个未完成的故事。

IF 1.8 3区生物学

Journal of Muscle Research and Cell Motility Pub Date : 2023-09-01 Epub Date: 2022-10-19 DOI: 10.1007/s10974-022-09629-x

Kenton P Arkill, C Charles Michel, Elizabeth V M Rider, Elise A Wood, Mathew O Small, Jennifer L E Brown, Abigail L Kinnaird

引用次数: 0

John Squire and the myosin thick filament structure in muscle. 约翰·斯奎尔和肌肉中的肌球蛋白粗丝结构。

IF 2.7 3区生物学

Journal of Muscle Research and Cell Motility Pub Date : 2023-09-01 Epub Date: 2023-04-26 DOI: 10.1007/s10974-023-09646-4

Kenneth A Taylor

引用次数: 0

Crystallisation and characterisation of muscle proteins: a mini-review. 肌肉蛋白的结晶和表征：一篇小型综述。

IF 2.7 3区生物学

Journal of Muscle Research and Cell Motility Pub Date : 2023-09-01 Epub Date: 2023-05-03 DOI: 10.1007/s10974-023-09648-2

Lata Govada, Naomi E Chayen

引用次数: 1

Zebrafish as a model for cardiac disease; Cryo-EM structure of native cardiac thin filaments from Danio Rerio. 斑马鱼作为心脏病的模型；Danio Rerio天然心脏细丝的冷冻电镜结构。

IF 1.8 3区生物学

Journal of Muscle Research and Cell Motility Pub Date : 2023-09-01 Epub Date: 2023-07-22 DOI: 10.1007/s10974-023-09653-5

Marston Bradshaw, John M Squire, Edward Morris, Georgia Atkinson, Rebecca Richardson, Jon Lees, Massimo Caputo, Giulia M Bigotti, Danielle M Paul

{"title":"Zebrafish as a model for cardiac disease; Cryo-EM structure of native cardiac thin filaments from Danio Rerio.","authors":"Marston Bradshaw, John M Squire, Edward Morris, Georgia Atkinson, Rebecca Richardson, Jon Lees, Massimo Caputo, Giulia M Bigotti, Danielle M Paul","doi":"10.1007/s10974-023-09653-5","DOIUrl":"10.1007/s10974-023-09653-5","url":null,"abstract":"Actin, tropomyosin and troponin, the proteins that comprise the contractile apparatus of the cardiac thin filament, are highly conserved across species. We have used cryo-EM to study the three-dimensional structure of the zebrafish cardiac thin and actin filaments. With 70% of human genes having an obvious zebrafish orthologue, and conservation of 85% of disease-causing genes, zebrafish are a good animal model for the study of human disease. Our structure of the zebrafish thin filament reveals the molecular interactions between the constituent proteins, showing that the fundamental organisation of the complex is the same as that reported in the human reconstituted thin filament. A reconstruction of zebrafish cardiac F-actin demonstrates no deviations from human cardiac actin over an extended length of 14 actin subunits. Modelling zebrafish homology models into our maps enabled us to compare, in detail, the similarity with human models. The structural similarities of troponin-T in particular, a region known to contain a hypertrophic cardiomyopathy 'hotspot', confirm the suitability of zebrafish to study these disease-causing mutations.","PeriodicalId":16422,"journal":{"name":"Journal of Muscle Research and Cell Motility","volume":" ","pages":"179-192"},"PeriodicalIF":1.8,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10542308/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10229245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Compensatory hemodynamic changes in response to central hypovolemia in humans: lower body negative pressure: updates and perspectives. 代偿性血流动力学改变对中枢性低血容量的反应:下体负压:最新进展和观点。

IF 2.7 3区生物学

Journal of Muscle Research and Cell Motility Pub Date : 2023-06-01 DOI: 10.1007/s10974-022-09635-z

Nandu Goswami

{"title":"Compensatory hemodynamic changes in response to central hypovolemia in humans: lower body negative pressure: updates and perspectives.","authors":"Nandu Goswami","doi":"10.1007/s10974-022-09635-z","DOIUrl":"https://doi.org/10.1007/s10974-022-09635-z","url":null,"abstract":"Central hypovolemia is accompanied by hemodynamic compensatory responses. Understanding the complex systemic compensatory responses to altered hemodynamic patterns during conditions of central hypovolemia-as induced by standing up and/or lower body negative pressure (LBNP)-in humans are important. LBNP has been widely used to understand the integrated physiological responses, which occur during sit to stand tests (orthostasis), different levels of hemorrhages (different levels of LBNP simulate different amount of blood loss) as well as a countermeasure against the cephalad fluid shifts which are seen during spaceflight. Additionally, LBNP application (used singly or together with head up tilt, HUT) is useful in understanding the physiology of orthostatic intolerance. The role seasonal variations in hormonal, autonomic and circulatory state play in LBNP-induced hemodynamic responses and LBNP tolerance as well as sex-based differences during central hypovolemia and the adaptations to exercise training have been investigated using LBNP. The data generated from LBNP studies have been useful in developing better models for prediction of orthostatic tolerance and/or for developing countermeasures. This review examines how LBNP application influences coagulatory parameters and outlines the effects of temperature changes on LBNP responses. Finally, the review outlines how LBNP can be used as innovative teaching tool and for developing research capacities and interests of medical students and students from other disciplines such as mathematics and computational biology.","PeriodicalId":16422,"journal":{"name":"Journal of Muscle Research and Cell Motility","volume":"44 2","pages":"89-94"},"PeriodicalIF":2.7,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10329599/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10191788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Extreme Physiology Extreme Tolerance to Hypoxia, Hypercapnia, and Pain in the Naked Mole-Rat. 裸鼹鼠对缺氧、高碳酸血症和疼痛的极端耐受性。

IF 2.7 3区生物学

Journal of Muscle Research and Cell Motility Pub Date : 2023-06-01 DOI: 10.1007/s10974-022-09623-3

Thomas J Park, Jane Reznick

{"title":"Extreme Physiology Extreme Tolerance to Hypoxia, Hypercapnia, and Pain in the Naked Mole-Rat.","authors":"Thomas J Park, Jane Reznick","doi":"10.1007/s10974-022-09623-3","DOIUrl":"https://doi.org/10.1007/s10974-022-09623-3","url":null,"abstract":"Challenging environmental conditions can drive the evolution of extreme physiological traits. The naked mole-rat has evolved to survive and thrive in a low oxygen, high carbon dioxide environment that would be deadly to humans and most other mammals. The naked mole-rat's lifestyle is unusual in that this species combines subterranean living and living in large, social groups of up to 300 + individuals. Many respiring animals in a closed environment can lead to depletion of oxygen (hypoxia) and accumulation of carbon dioxide (hypercapnia). Naked mole-rats display a variety of physiological traits that negate the adverse effects of living in this atmosphere. For hypoxia tolerance, naked mole-rats have a low resting metabolism, high affinity hemoglobin, intrinsic brain tolerance, the ability to use fructose for anaerobic glycolysis, and the ability to enter a low energy, suspended animation-like state. For hypercapnia tolerance, these animals have a mutation in a voltage gated sodium channel that effectively eliminates neuronal responses to tissue acidosis. In other mammals, acidosis from exposure to high concentrations of carbon dioxide induces pain and pulmonary edema. Understanding these mechanisms of extreme physiology is not only inherently interesting, but it may lead to biomedical breakthroughs in research on heart attacks, strokes, and pain pathologies.","PeriodicalId":16422,"journal":{"name":"Journal of Muscle Research and Cell Motility","volume":"44 2","pages":"61-72"},"PeriodicalIF":2.7,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10329625/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10193085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Utility of the burmese Python as a model for studying plasticity of extreme physiological systems. 将缅甸蟒蛇作为研究极端生理系统可塑性的模型。

IF 2.7 3区生物学

Journal of Muscle Research and Cell Motility Pub Date : 2023-06-01 Epub Date: 2022-11-01 DOI: 10.1007/s10974-022-09632-2

Yuxiao Tan, Thomas G Martin, Brooke C Harrison, Leslie A Leinwand

{"title":"Utility of the burmese Python as a model for studying plasticity of extreme physiological systems.","authors":"Yuxiao Tan, Thomas G Martin, Brooke C Harrison, Leslie A Leinwand","doi":"10.1007/s10974-022-09632-2","DOIUrl":"10.1007/s10974-022-09632-2","url":null,"abstract":"Non-traditional animal models present an opportunity to discover novel biology that has evolved to allow such animals to survive in extreme environments. One striking example is the Burmese python (Python molurus bivittatus), which exhibits extreme physiological adaptation in various metabolic organs after consuming a large meal following long periods of fasting. The response to such a large meal in pythons involves a dramatic surge in metabolic rate, lipid overload in plasma, and massive but reversible organ growth through the course of digestion. Multiple studies have reported the physiological responses in post-prandial pythons, while the specific molecular control of these processes is less well-studied. Investigating the mechanisms that coordinate organ growth and adaptive responses offers the opportunity to gain novel insight that may be able to treat various pathologies in humans. Here, we summarize past research on the post-prandial physiological changes in the Burmese python with a focus on the gastrointestinal tract, heart, and liver. Specifically, we address our recent molecular discoveries in the post-prandial python liver which demonstrate transient adaptations that may reveal new therapeutic targets. Lastly, we explore new biology of the aquaporin 7 gene that is potently upregulated in mammalian cardiac myocytes by circulating factors in post-prandial python plasma.","PeriodicalId":16422,"journal":{"name":"Journal of Muscle Research and Cell Motility","volume":"44 2","pages":"95-106"},"PeriodicalIF":2.7,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10149580/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10191786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lessons learned from comparative and extreme physiology. 从比较生理学和极限生理学中学到的经验。

IF 1.7 3区生物学

Journal of Muscle Research and Cell Motility Pub Date : 2023-06-01 Epub Date: 2023-03-31 DOI: 10.1007/s10974-023-09645-5

Avril Somlyo

引用次数: 0

Determinants of maximal oxygen consumption. 最大耗氧量的决定因素。

IF 2.7 3区生物学

Journal of Muscle Research and Cell Motility Pub Date : 2023-06-01 DOI: 10.1007/s10974-022-09636-y

Peter D Wagner

{"title":"Determinants of maximal oxygen consumption.","authors":"Peter D Wagner","doi":"10.1007/s10974-022-09636-y","DOIUrl":"https://doi.org/10.1007/s10974-022-09636-y","url":null,"abstract":"This article lays out the determinants of maximal O2 consumption (VO2max) achieved during high intensity endurance exercise. It is not a traditional topical review but rather an educational essay that intertwines chance observations made during an unrelated research project with a subsequent program of stepwise thought, analysis and experimentation to reveal how O2 is delivered to and used by the mitochondria. The centerpiece is the recognition that O2 is delivered by an inter-dependent system of transport components functioning as a \"bucket brigade\", made up of the lungs, heart, blood and circulation, and the muscles themselves, each of which affects O2 transport by similar amounts as they change. There is thus no single \"limiting factor\" to VO2max. Moreover, each component is shown to quantitatively affect the performance of the others. Mitochondrial respiration is integrated into the O2 transport system analysis to reveal its separate contribution to VO2max, and to show that mitochondrial PO2 at VO2max must be extremely low. Clinical application of the O2 transport systems analysis is described to separate central cardiopulmonary from peripheral tissue contributions to exercise limitation, illustrated by a study of patients with COPD. Finally, a short discussion of why muscles operating maximally must endure an almost anoxic state is offered. The hope is that in sum, both the increased understanding of O2 transport and the scientific approach to achieving that understanding described in the review can serve as a model for solving other complex problems going forward.","PeriodicalId":16422,"journal":{"name":"Journal of Muscle Research and Cell Motility","volume":"44 2","pages":"73-88"},"PeriodicalIF":2.7,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10544120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4