Journal of microbiological methods最新文献

{"title":"Deciphering microbial diversity and predicting metabolic functionalities in fermented pigmented rice water using culture-independent characterization","authors":"Shruti Mishra ,&nbsp;Asif Ali Vadakkethil ,&nbsp;Mir Asif Iquebal ,&nbsp;Sarika Jaiswal ,&nbsp;Dinesh Kumar ,&nbsp;Bhim Pratap Singh ,&nbsp;Said Ajlouni ,&nbsp;C. Senaka Ranadheera ,&nbsp;S. Chakkaravarthi","doi":"10.1016/j.mimet.2025.107295","DOIUrl":"10.1016/j.mimet.2025.107295","url":null,"abstract":"<div><div>Fermented rice water is gaining importance lately due to its traditional food culture and potential beneficial effects. Flavored fermented rice water (FFRW) produced from pigmented rice varieties, viz., black, brown, and red, is shown to have rich nutritional and functional profiles. However, the microbiota in this spontaneously fermented beverage is scantly known. Hence, this study aimed to explore the total bacterial and fungal diversity using 16S rRNA and Internal Transcribed Spacer (ITS) sequencing, respectively, along with the phytochemicals and their metabolites produced/utilized during storage. The bacterial diversity showed significant differences (<em>p</em> &lt; 0.05) in black FFRW while depicting stability for brown- and red-FFRW on the 0th day and 30th day of refrigerated storage. Lactic acid bacteria (LAB) like <em>Weissella</em> were abundantly recorded; similarly, fungal diversity showed dominance of various yeasts. Predictive functional/metabolic pathways suggested 23 pathways of which the predominant were metabolism amino acids like branched-chain amino acids (BCAAs) viz., leucine, valine, and isoleucine, aromatic amino acids such as tryptophan, and metabolites of glycan biosynthesis, polyphenols, lipids, cofactors and vitamins. KEGG pathways revealed a shift in microbial metabolism from amino acid degradation pathways dominating on day 0 to carbohydrate and fatty acid metabolism by day 30. Enzymes like lactate dehydrogenase showed increased abundance by the 30th day, particularly in red and black-FFRW. The untargeted profiling showed that brown FFRW had more polyphenol-related compounds, followed by black and red FFRW. Decrements in the compounds were detected on the 30th day of storage compared to the 0th day. The findings provide insights into the microbial diversity, metabolic potential, and phytochemical composition of FFRW, supporting its potential as a functional beverage.</div></div>","PeriodicalId":16409,"journal":{"name":"Journal of microbiological methods","volume":"239 ","pages":"Article 107295"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145370280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid qualitative identification and application of six common lactic acid bacteria based on surface enhanced Raman spectroscopy","authors":"Huihui Zhi ,&nbsp;Shijie Liu ,&nbsp;Yueyu Bai ,&nbsp;Miaoyun Li ,&nbsp;Dong Liang ,&nbsp;Lijun Zhao ,&nbsp;Jong-Hoon Lee ,&nbsp;Zihou Liu ,&nbsp;Qian Ding ,&nbsp;Manhong Zhan ,&nbsp;Lingxia Sun ,&nbsp;Yangyang Ma ,&nbsp;Yaodi Zhu","doi":"10.1016/j.mimet.2025.107312","DOIUrl":"10.1016/j.mimet.2025.107312","url":null,"abstract":"<div><div>To achieve rapid identification and detection of lactic acid bacteria (LAB) from different genera and species, six types of lactic acid bacteria were selected as the research objects, including <em>Lactobacillus plantarum</em> L1, <em>Lactobacillus gasseri</em> Y2, <em>Lactobacillus sakei</em> C3, <em>Pediococcus pentosaceus</em> W6, <em>Streptococcus lactis</em> S18 and <em>Pediococcus lactis</em> R9. Silver nanoparticle (AgNPs) were prepared as the SERS substrate material. The SERS spectra of the six LAB strains were analyzed, and a classification and identification model was constructed.The results showed that AgNPs, as the SERS substrate material, exhibited good signal enhancement performance and excellent reproducibility (<em>RSD</em> &lt; 6 %), which could clearly distinguish the differences in SERS spectral peaks among the six LAB strains. Through Principal Component Analysis (PCA) and Linear Discriminant Analysis (LDA), Various types of lactic acid bacteria can be fully visualized for classification and identification, with a model accuracy rate of 100 %. This study proposes a potential technical solution for quick qualitative identification of lactic acid bacteria.</div></div>","PeriodicalId":16409,"journal":{"name":"Journal of microbiological methods","volume":"239 ","pages":"Article 107312"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145452150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A rapid and efficient method for visualisation of microbial biofilms on natural, and industrially- and medically-relevant surfaces, using field emission- scanning electron microscopy","authors":"Abhinaba Chakraborty,&nbsp;Bomba Dam","doi":"10.1016/j.mimet.2025.107315","DOIUrl":"10.1016/j.mimet.2025.107315","url":null,"abstract":"<div><div>A rapid and efficient sample preparation method for visualising surface-associated microbial biofilms using Field Emission-Scanning Electron Microscopy (FE-SEM) was developed by optimising the fixative concentration and dehydration process. First, different concentrations of the fixative, glutaraldehyde (5–50 %), were tested on <em>Escherichia coli</em> biofilms formed on smooth glass surface for a fixation period of 30 min, followed by a 10-min dehydration each in increasing grades (10–90 %, with 10 % increment) of alcohol. The highest (50 %) glutaraldehyde concentration resulted in the sharpest biofilm micrographs. Further, the incubation period in each alcohol grade was reduced to 2 min to lower the sample preparation time. Improved imaging using the developed protocol was quantified using a newly developed metric, ‘Cellular Integrity Index’ (CII), which evaluates the morphological integrity of biofilm-cells. The protocol preserved the cellular integrity of individual biofilm-associated cells of <em>Aeromonas hydrophila</em>, <em>A. salmonicida</em>, <em>Pseudomonas fluorescens</em>, and <em>Bacillus mycoides</em>. The optimised method generated distinctive high-resolution micrographs of <em>E. coli</em> biofilms pre-formed on different medically-, industrially-, and environmentally-relevant surfaces, such as polypropylene plastic, catheter, and paper, all with high CII values (95–97 %) with least deformation. Further, the method was used for visualising naturally-formed biofilms on poultry ceca, plant roots, and rock surfaces with impeccable clarity, even effectively resolving different microorganisms, like fungus, algae and bacteria Thus, the developed method will be a valuable asset for any research dealing with the visualisation of naturally-formed or laboratory-developed biofilms on any sort-of surface, be it of a single individual or mixed species, thus enriching environmental, industrial, and medical research.</div></div>","PeriodicalId":16409,"journal":{"name":"Journal of microbiological methods","volume":"239 ","pages":"Article 107315"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145426513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid estimation of bacterial abundance in mouse feces by flow cytometry with SYBR green I and counting beads","authors":"Virginia A. Piqueras,&nbsp;Silvia G. Correa","doi":"10.1016/j.mimet.2025.107323","DOIUrl":"10.1016/j.mimet.2025.107323","url":null,"abstract":"<div><h3>Aims</h3><div>To present a standardized and reproducible flow cytometry (FCM) protocol for the rapid estimation of bacterial abundance in mouse fecal samples using SYBR Green I and counting beads.</div></div><div><h3>Methods and results</h3><div>The protocol uses nucleic acid staining and reference beads to estimate SYBR Green I-positive events compatible with bacterial cells. Optimization included titration of dye and beads, gating strategy, and acquisition settings. The method was applied to fecal samples from male and female C57BL/6 and Foxp3-EGFP mice under specific-pathogen-free conditions. Measurements performed seven days apart showed high repeatability. Significant differences in bacterial abundance were observed between mouse strains, though not between sexes.</div></div><div><h3>Conclusions</h3><div>This FCM-based method enables rapid and consistent estimation of bacterial-like particles in fecal suspensions, though it does not distinguish live/dead cells or taxonomic identity.</div></div><div><h3>Significance</h3><div>The protocol provides a rapid, accessible approach for comparative or screening studies of gut microbiota dynamics, especially where detailed compositional analysis is not required or feasible.</div></div>","PeriodicalId":16409,"journal":{"name":"Journal of microbiological methods","volume":"239 ","pages":"Article 107323"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145505028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microscale assay for the quantification of total polysaccharides to estimate extracellular polymeric substances (EPS) in soil","authors":"G. Bogar ,&nbsp;J.T. Lennon ,&nbsp;H.M. Vander Stel ,&nbsp;S.E. Evans","doi":"10.1016/j.mimet.2025.107324","DOIUrl":"10.1016/j.mimet.2025.107324","url":null,"abstract":"<div><div>We present an inexpensive and practical microscale spectrophotometric acid-phenol assay to quantify polysaccharides in aqueous solution. The instant reaction within disposable materials is designed and benchmarked for use in quantifying extracellular polymeric substances (EPS) in soils. Precipitation recovery was high across expected ranges of soil EPS content.</div></div>","PeriodicalId":16409,"journal":{"name":"Journal of microbiological methods","volume":"239 ","pages":"Article 107324"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145505054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"“Evaluating the susceptibility of MBL carbapenemase-producing Enterobacteriaceae and Pseudomonas spp. to ceftazidime/avibactam plus aztreonam and Cefiderocol: A synergy study and susceptibility profile”","authors":"Ana Collazos Blanco ,&nbsp;Ana Belén García Sáez ,&nbsp;Carolina Plaza Cristobal ,&nbsp;Ismael Darid y Cerón ,&nbsp;Alicia Macías Valcayo ,&nbsp;María Isabel Zamora-Cintas ,&nbsp;María Simón Sacristán","doi":"10.1016/j.mimet.2025.107311","DOIUrl":"10.1016/j.mimet.2025.107311","url":null,"abstract":"<div><div>This study assessed the in vitro susceptibility of aztreonam/avibactam (ATM/AVI) and the synergy analyses against 38 clinical carbapenemase-producing strains (31 Enterobacterales and 7 <em>Pseudomonas</em> spp.) collected over two years. Susceptibility testing were performed followed EUCAST guidelines and synergy was studied using the gradient strip stacking method and FICI analysis.</div><div>Synergy between aztreonam (AZT) and Ceftazidime/Avibactam (CZA) was observed in 58,1 % of enterobacterales strains overall, with stronger synergy (100 %) in aztreonam-resistant group compared to aztreonam-susceptible ones (18.8 %). ATM/AVI Showed 100 % susceptibility among Enterobacterales with the epsilon test diffusion method.</div><div>Cefiderocol (FDC) demonstrated high activity, with 93.5 % susceptibility in Enterobacterales and 100 % in <em>Pseudomonas</em> spp.</div><div>The findings support ATM/AVI as a promising option for MBL-producing Enterobacterales, while the role of aztreonam/avibactam in the clinical treatment of <em>Pseudomonas</em> spp. MBL-type carbapenemases producers is still uncertain. Cefiderocol remains highly effective against MBL-producing Enterobacterales and <em>Pseudomonas</em> spp.</div></div>","PeriodicalId":16409,"journal":{"name":"Journal of microbiological methods","volume":"239 ","pages":"Article 107311"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145390316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Suppression/competition PCR: A novel method to minimize unwanted amplicons in metabarcoding, with applications to parasite detection in fecal samples","authors":"Justin Mark Carpani ,&nbsp;John R. Barta ,&nbsp;Rebecca A. Guy","doi":"10.1016/j.mimet.2025.107320","DOIUrl":"10.1016/j.mimet.2025.107320","url":null,"abstract":"<div><div>Metabarcoding is widely used for detecting microorganisms in fecal samples, but its effectiveness is often limited by the co-amplification of abundant non-target DNA. In this study, a novel metabarcoding assay was developed to amplify a near-complete 18S rRNA gene fragment suitable for long-read nanopore sequencing, enhancing taxonomic resolution. The primers were optimized to maximize detection of parasitic taxa while minimizing off-target amplification of bacterial and archaeal sequences, thereby improving assay specificity. In this study, the 18S metabarcoding assay worked well on clinical fecal samples containing clinically relevant levels of parasites. However, analysis of ungulate fecal samples revealed that fungal and plant sequences vastly outnumbered other eukaryotic taxa in many samples, obscuring the detection of low-abundance protozoan and helminth parasites. To address this, Suppression/Competition PCR was developed, a novel method that selectively reduces amplification of unwanted DNA. This approach reduced fungal and plant reads by over 99 %, enabling sequences from other taxa to comprise an average of over 98 % of total reads as opposed to an initial 36 %. Utilizing this newly-developed metabarcoding assay in either the standard or Suppression/Competition configuration on fecal DNA extracts from a range of host species, parasites of interest such as <em>Cryptosporidium</em> sp., <em>Cyclospora cayetanensis</em>, <em>Blastocystis</em> sp., <em>Entamoeba</em> sp., <em>Eimeria</em> sp., <em>Ancylostoma</em> sp., and <em>Toxocara</em> sp. were detected, demonstrating its broad applicability.</div></div>","PeriodicalId":16409,"journal":{"name":"Journal of microbiological methods","volume":"239 ","pages":"Article 107320"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145452194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determining the best method for assessing microbiological contamination on flexible endoscopes without a working channel: a pilot study","authors":"Yana Halmans ,&nbsp;David J. Wellenstein ,&nbsp;Michael Romijn ,&nbsp;Suzan A.J. Cremers ,&nbsp;Joost Hopman ,&nbsp;Robert P. Takes ,&nbsp;Guido B. van den Broek","doi":"10.1016/j.mimet.2025.107328","DOIUrl":"10.1016/j.mimet.2025.107328","url":null,"abstract":"<div><div>Background: Flexible endoscopes (FEs) without a working channel can become extensively contaminated. Adequate reprocessing is critical and the quality of these processes can be verified by microbiological sampling. This study aimed to determine the ability to detect colony-forming units (CFUs) after sampling used FEs without a working channel for four different sampling techniques. Methods: FEs without a working channel were collected after clinical use. Manual pre-cleaning with tap water was performed. A sample was collected from the distal 8-10 cm and tip of the FE by one of four sampling techniques: rolling over a Trypticase Soy Agar with tween and lecithin plate (group 1), rolling over a Plate Count Agar + additives (group 2), swab technique (group 3) or broth technique (group 4). After incubation, a CFU count was performed to assess microbiological contamination. Results: 160 FEs without a working channel were evenly distributed among the four sampling groups. The CFUs on the FEs were classified into four groups: 0 CFUs, 1–50 CFUs, 50–500 CFUs and &gt; 500 CFUs. All four sampling techniques showed a variable number of CFUs, ranging from 0 to uncountable CFUs (i.e., &gt;500) (<em>p</em> ≤0.001). No statistically significant difference was found in how often &gt;0 CFUs were found between the sampling techniques (<em>p</em> = 0.21). Conclusions: All four sampling techniques were able to detect CFUs on used FEs without a working channel, indicating all four sampling techniques can be used for assessing microbiological contamination.</div></div>","PeriodicalId":16409,"journal":{"name":"Journal of microbiological methods","volume":"239 ","pages":"Article 107328"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145522784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Strain-specific nitrogen source preferences and pH-controlled fermentation of Lactobacillus gasseri, L. acidophilus, and L. helveticus for industrial probiotic production","authors":"Feng Hang ,&nbsp;Yangyang Shi ,&nbsp;Shaoquan Yan ,&nbsp;Rui Li","doi":"10.1016/j.mimet.2025.107307","DOIUrl":"10.1016/j.mimet.2025.107307","url":null,"abstract":"<div><div>This study aimed to identify growth-limiting factors and optimize fermentation conditions for three probiotic lactobacilli (<em>Lactobacillus gasseri</em>, <em>L. acidophilus</em>, and L. <em>helveticus</em>) that show suboptimal performance in industrial processes, with the goal of enhancing cellular proliferation and production efficiency. Four commercial nitrogen sources were evaluated for their impact on viable cell counts, pH, and morphology. Scale-up fermentation was subsequently conducted in a 100-L pH-stat fermenter to examine pH-dependent growth kinetics. Results revealed strain-specific nitrogen preferences: <em><strong>L</strong>. helveticus</em> exhibited robust growth across all yeast extracts, whereas L. <em>acidophilus</em> and L. <em>gasseri</em> displayed significantly higher viable cell counts (<em>P</em> &lt; 0.05) in yeast extract 503 (rich in nucleotides) compared to other nitrogen sources, accompanied by reduced cell lengths. Furthermore, pH-stat fermentation at 4.5 promoted growth (2.96–3.47 × 10⁹ CFU/mL) and shortened cell morphology for all three strains. These findings reveal how nucleotides in nitrogen sources and acidic pH regulate growth of lactic acid bacteria via nitrogen-pH-morphology interplay, offering important implications for industrial-scale production optimization.</div></div>","PeriodicalId":16409,"journal":{"name":"Journal of microbiological methods","volume":"239 ","pages":"Article 107307"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145345697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An improved immunochromatographic test strip that sensitively detects heat-labile enterotoxin produced by enterotoxigenic Escherichia coli","authors":"Nana Fujimoto ,&nbsp;Emika Inoue ,&nbsp;Nonoka Yokomizo ,&nbsp;Sakura Hayashi ,&nbsp;Mana Yoneyama ,&nbsp;Tomoko Kohda ,&nbsp;Masahiro Kusumoto ,&nbsp;Hideyuki Arimitsu","doi":"10.1016/j.mimet.2025.107299","DOIUrl":"10.1016/j.mimet.2025.107299","url":null,"abstract":"<div><div>Enterotoxigenic <em>Escherichia coli</em> (ETEC) can cause watery diarrhea not only in humans but also in domestic animals, for example, causing post-weaning diarrhea in piglets. Because the major causative factors of ETEC are heat-labile enterotoxin (LT) and heat-stable enterotoxin (ST), rapid detection methods are required for these toxins. We previously reported a prototype immunochromatographic (IC) test strip for LT comprising a mouse monoclonal antibody (mAb) and rabbit polyclonal antibody. However, the toxin detection limit of this test strip was insufficient, and the test sample bacteria needed to be cultured overnight with lincomycin supplementation to enhance LT production. Moreover, no IC test strips were available for ST. Therefore, we attempted to create a chimera protein of the B subunit of LT (LTB) and ST and develop mAbs against both LTB and ST through immunization of mice. Although nine antigen-specific mAb clones were obtained, all were LTB-specific. IC test strips prepared with the mAb 31D11 and mAb 34D4 pair were able to detect 0.15 ng/150 μL of purified LT. In addition, this IC test strip was able to detect LT in 6-h culture supernatants of clinical isolates from swine and human without requiring lincomycin supplementation. Quantification of LT levels using sandwich ELISA corroborated the IC results, indicating that the improved IC test strip enabled the detection of LT at lower concentrations and with shorter culture times than the previous method, without the need for supplements. This test strip will be useful for ETEC detection in the food hygiene and livestock hygiene fields.</div></div>","PeriodicalId":16409,"journal":{"name":"Journal of microbiological methods","volume":"239 ","pages":"Article 107299"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145318237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}