多重等位基因特异性PCR和DNA色谱法鉴定肠沙门氏菌血清型鼠伤寒杆菌及其单相变异的基因型。

IF 1.7 4区生物学 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of microbiological methods Pub Date : 2025-01-01 DOI:10.1016/j.mimet.2024.107082

Nobuo Arai , Yukino Tamamura-Andoh , Taketoshi Iwata , Ayako Watanabe-Yanai , Masahiro Kusumoto

引用次数: 0

摘要

我们建立了一种结合多重等位基因特异性PCR和DNA层析的简易肠沙门氏菌亚种基因分型方法。肠炎血清型鼠伤寒菌及其单相变种。该方法可用于估计分离株的遗传背景，并可方便地从这些血清型中鉴定出几种流行支系。

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Multiplex allele-specific PCR and DNA chromatography to identify genotypes of Salmonella enterica serovar Typhimurium and its monophasic variants

We constructed a simple genotyping method combining multiplex allele-specific PCR and DNA chromatography for Salmonella enterica subsp. enterica serovar Typhimurium and its monophasic variant. The developed method can be used to estimate the genetic background of isolates, and it facilitates easy identification of several epidemic clades among these serotypes.

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来源期刊

Journal of microbiological methods 生物-生化研究方法

CiteScore

4.30

自引率

4.50%

发文量

151

审稿时长

29 days

期刊介绍： The Journal of Microbiological Methods publishes scholarly and original articles, notes and review articles. These articles must include novel and/or state-of-the-art methods, or significant improvements to existing methods. Novel and innovative applications of current methods that are validated and useful will also be published. JMM strives for scholarship, innovation and excellence. This demands scientific rigour, the best available methods and technologies, correctly replicated experiments/tests, the inclusion of proper controls, calibrations, and the correct statistical analysis. The presentation of the data must support the interpretation of the method/approach. All aspects of microbiology are covered, except virology. These include agricultural microbiology, applied and environmental microbiology, bioassays, bioinformatics, biotechnology, biochemical microbiology, clinical microbiology, diagnostics, food monitoring and quality control microbiology, microbial genetics and genomics, geomicrobiology, microbiome methods regardless of habitat, high through-put sequencing methods and analysis, microbial pathogenesis and host responses, metabolomics, metagenomics, metaproteomics, microbial ecology and diversity, microbial physiology, microbial ultra-structure, microscopic and imaging methods, molecular microbiology, mycology, novel mathematical microbiology and modelling, parasitology, plant-microbe interactions, protein markers/profiles, proteomics, pyrosequencing, public health microbiology, radioisotopes applied to microbiology, robotics applied to microbiological methods,rumen microbiology, microbiological methods for space missions and extreme environments, sampling methods and samplers, soil and sediment microbiology, transcriptomics, veterinary microbiology, sero-diagnostics and typing/identification.