Journal of Molecular Microbiology and Biotechnology最新文献_第3页

Relationship between Adaptive Changing of Lysophosphatidylethanolamine Content in the Bacterial Envelope and Ampicillin Sensitivity of Yersinia pseudotuberculosis. 假结核耶尔森菌包膜溶血磷脂酰乙醇胺含量的适应性变化与氨苄西林敏感性的关系

IF 1.2

Journal of Molecular Microbiology and Biotechnology Pub Date : 2018-01-01 Epub Date: 2019-03-07 DOI: 10.1159/000497180

Nina Sanina, Lyudmila Pomazenkova, Svetlana Bakholdina, Natalia Chopenko, Anna Zabolotnaya, Vladimir Reutov, Anna Stenkova, Evgeniya Bystritskaya, Mikhail Bogdanov

{"title":"Relationship between Adaptive Changing of Lysophosphatidylethanolamine Content in the Bacterial Envelope and Ampicillin Sensitivity of Yersinia pseudotuberculosis.","authors":"Nina Sanina, Lyudmila Pomazenkova, Svetlana Bakholdina, Natalia Chopenko, Anna Zabolotnaya, Vladimir Reutov, Anna Stenkova, Evgeniya Bystritskaya, Mikhail Bogdanov","doi":"10.1159/000497180","DOIUrl":"https://doi.org/10.1159/000497180","url":null,"abstract":"The low permeability of porin channels is the possible reason for Gram-negative bacterial resistance to antibiotics. The adaptive accumulation of lysophosphatidylethanolamine (LPE) in Yersinia pseudotuberculosis induces conformational changes of OmpF porin that may hinder the transport of antibiotics through this channel. The present study was aimed to test whether the changes in LPE content affect the resistance of bacteria to ampicillin. The addition of glucose to the culture medium was shown to simultaneously increase the level of LPE and minimum inhibitory concentration (MIC) for ampicillin of Y. pseudotuberculosis cells 6- and 2-fold, respectively. However, the coadministration of glucose and polyphenol extract from buckwheat husks reduced the content of LPE 2-fold and restored MIC to the control value. Thus, PBEH can be used as antibiotic adjuvant to improve an antibiotic's ability to cross the outer membrane. The present work demonstrated: (i) the role of adaptive changes in the lipid composition of Y. pseudotuberculosis in the development of antibiotic resistance, and (ii) the promising use of PBEH in combination therapy to increase the susceptibility of Gram-negative bacteria to the conventional β-lactam antibiotics, probably attenuating in vivo a previously demonstrated effect of LPE on the conformation and function of the OmpF channel.","PeriodicalId":16370,"journal":{"name":"Journal of Molecular Microbiology and Biotechnology","volume":"28 5","pages":"236-239"},"PeriodicalIF":1.2,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000497180","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37208186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytochrome bcc-aa3 Oxidase Supercomplexes in the Aerobic Respiratory Chain of Streptomyces coelicolor A3(2). 彩色链霉菌A3有氧呼吸链中的细胞色素bcc-aa3氧化酶超复合体(2)。

IF 1.2

Journal of Molecular Microbiology and Biotechnology Pub Date : 2018-01-01 Epub Date: 2019-03-12 DOI: 10.1159/000496390

Dörte Falke, Marco Fischer, Bianca Biefel, Christian Ihling, Claudia Hammerschmidt, Kevin Reinefeld, Alexander Haase, Andrea Sinz, R Gary Sawers

{"title":"Cytochrome bcc-aa3 Oxidase Supercomplexes in the Aerobic Respiratory Chain of Streptomyces coelicolor A3(2).","authors":"Dörte Falke, Marco Fischer, Bianca Biefel, Christian Ihling, Claudia Hammerschmidt, Kevin Reinefeld, Alexander Haase, Andrea Sinz, R Gary Sawers","doi":"10.1159/000496390","DOIUrl":"https://doi.org/10.1159/000496390","url":null,"abstract":"Streptomyces coelicolor A3(2), an obligately aerobic, oxidase-positive, and filamentous soil bacterium, lacks a soluble cytochrome c in its respiratory chain, having instead a membrane-associated diheme c-type cytochrome, QcrC. This necessitates complex formation to allow electron transfer between the cytochrome bcc and aa3 oxidase respiratory complexes. Combining genetic complementation studies with in-gel cytochrome oxidase activity staining, we demonstrate that the complete qcrCAB-ctaCDFE gene locus on the chromosome, encoding, respectively, the bcc and aa3 complexes, is required to manifest a cytochrome oxidase enzyme activity in both spores and mycelium of a qcr-cta deletion mutant. Blue-native-PAGE identified a cytochrome aa3 oxidase complex of approximately 270 kDa, which catalyzed oxygen-dependent diaminobenzidine oxidation without the requirement for exogenously supplied cytochrome c, indicating association with QcrC. Furthermore, higher molecular mass complexes were identified upon addition of soluble cytochrome c, suggesting the supercomplex is unstable and readily dissociates into subcomplexes lacking QcrC. Immunological and mass spectrometric analyses of active, high-molecular mass oxidase-containing complexes separated by clear-native PAGE identified key subunits of both the bcc complex and the aa3 oxidase, supporting supercomplex formation. Our data also indicate that the cytochrome b QcrB of the bcc complex is less abundant in spores compared with mycelium.","PeriodicalId":16370,"journal":{"name":"Journal of Molecular Microbiology and Biotechnology","volume":"28 6","pages":"255-268"},"PeriodicalIF":1.2,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000496390","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37223169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

The Extended C-Terminal α-Helix of the HypC Chaperone Restricts Recognition of Large Subunit Precursors by the Hyp-Scaffold Machinery during [NiFe]-Hydrogenase Maturation in Escherichia coli. 在大肠杆菌[NiFe]-氢化酶成熟过程中，HypC伴侣蛋白延伸的c端α-螺旋限制了HypC支架机制对大亚基前体的识别。

IF 1.2

Journal of Molecular Microbiology and Biotechnology Pub Date : 2018-01-01 Epub Date: 2018-07-11 DOI: 10.1159/000489929

Claudia Thomas, Mandy Waclawek, Kerstin Nutschan, Constanze Pinske, R Gary Sawers

{"title":"The Extended C-Terminal α-Helix of the HypC Chaperone Restricts Recognition of Large Subunit Precursors by the Hyp-Scaffold Machinery during [NiFe]-Hydrogenase Maturation in Escherichia coli.","authors":"Claudia Thomas, Mandy Waclawek, Kerstin Nutschan, Constanze Pinske, R Gary Sawers","doi":"10.1159/000489929","DOIUrl":"https://doi.org/10.1159/000489929","url":null,"abstract":"Members of the HypC protein family are chaperone-like proteins that play a central role in the maturation of [NiFe]-hydrogenases (Hyd). Escherichia coli has a second copy of HypC, called HybG, and, as a component of the HypDEF maturation scaffold, these proteins help synthesize the NiFe-cofactor and guide the scaffold to its designated hydrogenase large subunit precursor. HypC is required to synthesize active Hyd-1 and Hyd-3, while HybG facilitates Hyd-2 and Hyd-1 synthesis. To identify determinants on HypC that allow it to discriminate against Hyd-2, we made amino acid exchanges in 3 variable regions, termed VR1, VR2, and VR3, of HypC, that make it more similar to HybG. Region VR3 includes a HypC-specific C-terminal α-helical extension, and this proved particularly important in preventing the maturation of Hyd-2 by HypC. Truncation of this extension on HypC increased Hyd-2 activity in the absence of HybG, while retaining maturation of Hyd-3 and Hyd-1. Combining this truncation with amino acid exchanges in VR1 and VR2 of HypC negatively affected the synthesis of active Hyd-1. The C-terminus of E. coli HypC is thus a key determinant in hindering Hyd-2 maturation, while VR1 and VR2 appear more important for Hyd-1 matu-ration.","PeriodicalId":16370,"journal":{"name":"Journal of Molecular Microbiology and Biotechnology","volume":"28 2","pages":"87-97"},"PeriodicalIF":1.2,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000489929","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36304856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Comparative Proteomic Analysis of Propane Metabolism in Mycobacterium sp. Strain ENV421 and Rhodococcus sp. Strain ENV425. 分枝杆菌ENV421和红球菌ENV425丙烷代谢的蛋白质组学比较分析

IF 1.2

Journal of Molecular Microbiology and Biotechnology Pub Date : 2018-01-01 Epub Date: 2018-08-28 DOI: 10.1159/000490494

Peter Robert Tupa, Hisako Masuda

{"title":"Comparative Proteomic Analysis of Propane Metabolism in Mycobacterium sp. Strain ENV421 and Rhodococcus sp. Strain ENV425.","authors":"Peter Robert Tupa, Hisako Masuda","doi":"10.1159/000490494","DOIUrl":"https://doi.org/10.1159/000490494","url":null,"abstract":"While growing on propane as a sole source of carbon, many strains cometabolically degrade environmental pollutants, such as ethers and chlorinated hydrocarbons. To gain insights into the molecular basis behind such a high metabolic versatility of propanotrophs, we examined the propane-inducible protein expression patterns of 2 soil actinobacteria that are known to degrade a variety of ethers (i.e., Mycobacterium sp. strain ENV421 and Rhodococcus sp. strain ENV425). In both strains, soluble diiron monooxygenase(s), that would catalyze the first step of the pathway, were induced by propane. However, despite their phylogenetic similarity, different sets of additional putative propane oxygenases (e.g., cytochrome P450 and particulate methane monooxygenases) were overexpressed in the 2 strains. They also diverged in the expression of enzymes responsible for downstream reactions. This study revealed a diversity of expression of putative propane oxygenases, which may be responsible for xenobiotic degradation, as well as a variety of metabolic pathways for propane in these bacterial species.","PeriodicalId":16370,"journal":{"name":"Journal of Molecular Microbiology and Biotechnology","volume":"28 3","pages":"107-115"},"PeriodicalIF":1.2,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000490494","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36436882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Comparison of DNA Extraction Efficiency and Reproducibility of Different Aeration Diffuser Biofilms Using Bead-Beating Protocol. 不同曝气扩散器生物膜的DNA提取效率及可重复性比较。

IF 1.2

Journal of Molecular Microbiology and Biotechnology Pub Date : 2018-01-01 Epub Date: 2019-06-24 DOI: 10.1159/000500875

Pitiporn Asvapathanagul, Manel Garrido-Baserba, Betty H Olson, Hee-Deung Park, Deqiang Chen, Diego Rosso

{"title":"Comparison of DNA Extraction Efficiency and Reproducibility of Different Aeration Diffuser Biofilms Using Bead-Beating Protocol.","authors":"Pitiporn Asvapathanagul, Manel Garrido-Baserba, Betty H Olson, Hee-Deung Park, Deqiang Chen, Diego Rosso","doi":"10.1159/000500875","DOIUrl":"https://doi.org/10.1159/000500875","url":null,"abstract":"An existing bead-beating DNA extraction protocol was employed to compare the DNA extraction recovery and fragment quality of 6 different aeration diffuser biofilms. Escherichia coli, Gordonia amarae, and mixed liquor were used as controls. The fraction of total DNAbiofilm decreased monotonically with increasing number of beat beatings (BB) when the amount of DNA present was sufficient (>4 μgDNA/cm2), excluding the ceramic disk. While controls required only 2 BBs, 3 out of 5 BBs achieved ≥70% of total DNA (70.3 ± 1.7%) for 5 out of 6 biofilms. Quantitative polymerase chain reaction (PCR) analyses of 353 and 1,505 basepair (bp) amplicons from pure culture extracts showed target copy numbers were not degraded for the first 2 BBs, but the third BB decreased amplicon concentrations by 0.65 and 1.12 log for E. coli, and 0.39 and 0.40 log for G. amarae, respectively. The 353 bp fragment amplification from biofilm samples showed minimal degradation for the first 3 BBs. PCR and gel electrophoresis confirmed integrity of amplified 1,505 bp DNA fragments over the 5 BBs, except in the EDPM (75 mm diameter, tube) diffuser biofilm (4.98 ± 0.62 μgDNA/cm2). Taken together, this study showed type of diffuser membrane biofilms had no effects on extraction efficiency, but low DNA concentrations reduced extraction performance.","PeriodicalId":16370,"journal":{"name":"Journal of Molecular Microbiology and Biotechnology","volume":"28 6","pages":"293-304"},"PeriodicalIF":1.2,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000500875","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37097640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Understanding the Physiology of Liberibacter asiaticus: An Overview of the Demonstrated Molecular Mechanisms. 了解亚洲自由杆菌的生理学:已证实的分子机制综述。

IF 1.2

Journal of Molecular Microbiology and Biotechnology Pub Date : 2018-01-01 Epub Date: 2018-09-03 DOI: 10.1159/000492386

Janelle F Coyle, Graciela L Lorca, Claudio F Gonzalez

{"title":"Understanding the Physiology of Liberibacter asiaticus: An Overview of the Demonstrated Molecular Mechanisms.","authors":"Janelle F Coyle, Graciela L Lorca, Claudio F Gonzalez","doi":"10.1159/000492386","DOIUrl":"https://doi.org/10.1159/000492386","url":null,"abstract":"Citrus greening disease, or huanglongbing, may entirely eradicate all varieties of citrus cultivars worldwide in the near future. This disease is caused by non-cultivable bacteria of the genus Liberibacter; among them, the more pathogenic being Liberibacter asiaticus. The complexity of the host-pathogen relationship, associated with the impossibility of performing research using axenic cultures, has severely hindered the basic research on microbiology. Since its genome sequence was published in 2009, most of the scientific publications in the field were dedicated to in silico analysis and selection of targets to design early detection methods. The knowledge gained with these approaches felt short to articulate effective methods to control the disease progression. There is a critical need to understand the basic biology of bacteria to design effective strategies to inactivate central mechanisms of pathogenesis. In this review, we summarize the scientific progress made by studying L. asiaticus' biology through direct experimentation. The evidence collected thus far is not enough to understand L. -asiaticus' fundamental biology. It is imperiously necessary to increase the basic research to identify relevant biological clues to control citrus greening. The gained knowledge may also help to prevent potential catastrophic diseases in other crops of significant importance caused by other unculturable Liberibacter species.","PeriodicalId":16370,"journal":{"name":"Journal of Molecular Microbiology and Biotechnology","volume":"28 3","pages":"116-127"},"PeriodicalIF":1.2,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000492386","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36457485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Multidrug Resistance and the Predominance of blaCTX-M in Extended Spectrum Beta-Lactamase-Producing Enterobacteriaceae of Animal and Water Origin. 动物和水源产广谱β -内酰胺酶肠杆菌科细菌的多药耐药及blaCTX-M的优势

IF 1.2

Journal of Molecular Microbiology and Biotechnology Pub Date : 2018-01-01 Epub Date: 2019-01-04 DOI: 10.1159/000495409

Bilel Hassen, Senda Sghaier, Mohamed Salah Abbassi, Mohamed Amine Ferjani, Meriam Ben Said, Abdennaceur Hassen, Salah Hammami

{"title":"Multidrug Resistance and the Predominance of blaCTX-M in Extended Spectrum Beta-Lactamase-Producing Enterobacteriaceae of Animal and Water Origin.","authors":"Bilel Hassen, Senda Sghaier, Mohamed Salah Abbassi, Mohamed Amine Ferjani, Meriam Ben Said, Abdennaceur Hassen, Salah Hammami","doi":"10.1159/000495409","DOIUrl":"https://doi.org/10.1159/000495409","url":null,"abstract":"The aim of this work was the genetic characterization of cefotaxime-resistant enterobacteria from animals (53 samples), the surface water of rivers (17 samples), and wastewater treatment plants (43 samples) in Tunisia. A total of 48 (42.4%) cefotaxime-resistant isolates were recovered. An extended spectrum beta-lactamase (ESBL) phenotype with a positive double-disk synergy test (DDST) was exhibited by 34 (70.8%) and 14 (29.1%) isolates from water and animal origins, respectively. Isolates from water were identified as: Escherichia coli (n = 17), Hafnia spp. (n = 13), Citrobacter spp. (n = 1), Enterobacter cloacae (n = 1), Klebsiella pneumoniae (n = 1), and K. oxytoca (n = 1). Animal isolates were identified as: E. coli (n = 11), E. cloacae (n = 1), Hafnia spp. (n = 1), and K. pneumoniae (n = 1). PCR investigation of blaCTX-M, blaTEM, and blaSHV genes showed that amongst the 48 isolates with a positive DDST, 41 (87.5%) carried the blaCTX-M gene, 1 isolate harbored the blaSHV gene, and 1 isolate coharbored blaCTX-M with blaSHV genes. The class 1 and 2 integrons were detected in 27 (56.2%) and 1 (2%) isolates, respectively. Our study showed a significant occurrence of ESBL-producing enterobacteria in animals and aquatic environments with a predominance of blaCTX-M genes.","PeriodicalId":16370,"journal":{"name":"Journal of Molecular Microbiology and Biotechnology","volume":"28 4","pages":"201-206"},"PeriodicalIF":1.2,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000495409","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36826420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Subunit Composition of Ribosome in the yqgF Mutant Is Deficient in pre-16S rRNA Processing of Escherichia coli. yqgF突变体的核糖体亚基组成在大肠杆菌的前16s rRNA加工中存在缺陷。

IF 1.2

Journal of Molecular Microbiology and Biotechnology Pub Date : 2018-01-01 Epub Date: 2018-12-19 DOI: 10.1159/000494494

Tatsuaki Kurata, Shinobu Nakanishi, Masayuki Hashimoto, Masato Taoka, Toshiaki Isobe, Jun-Ichi Kato

引用次数: 3

Improved Production of Highly Active and Pure Human Creatine Kinase MB. 高活性和纯人肌酸激酶MB的改进生产。

IF 1.2

Journal of Molecular Microbiology and Biotechnology Pub Date : 2018-01-01 Epub Date: 2018-03-14 DOI: 10.1159/000486716

Sureyya Mert Selimoglu, Murat Kasap, Gurler Akpinar, Aynur Karadenizli, Abdul Mounem Wis, Uzay Gormus

{"title":"Improved Production of Highly Active and Pure Human Creatine Kinase MB.","authors":"Sureyya Mert Selimoglu, Murat Kasap, Gurler Akpinar, Aynur Karadenizli, Abdul Mounem Wis, Uzay Gormus","doi":"10.1159/000486716","DOIUrl":"https://doi.org/10.1159/000486716","url":null,"abstract":"Human creatine kinase MB (hCKMB) is one of the most preferred biomarkers used for the diagnosis of acute coronary syndrome due to its high sensitivity and specificity. The increasing need for highly purified and biologically active hCKMB in the field of diagnostics makes its production valuable. Currently, the production of hCKMB is mainly achieved in methylotrophic yeast, Pichia pastoris, because the production in Escherichia coli is challenging and generally yields an inactive enzyme with a low quantity. With the aim of finding the best way for the high-yield production of active hCKMB in E. coli, an efficient strategy was developed using a construct allowing tandem expression of each subunit with 2 different tags. The strategy allowed the efficient expression and separate characterization of each subunit and 1-step purification of the heterodimeric protein into homogeneity. The heterodimeric protein displayed more than 11-fold greater specific activity than the commercially available one. The production strategy described in this study shows a clear advantage over the currently used ones and can be made available not only for laboratory scale production but also for commercial production. Our study is also a well-suited example for the studies in which novel protein expression strategies are needed to achieve greater yields with higher purities.","PeriodicalId":16370,"journal":{"name":"Journal of Molecular Microbiology and Biotechnology","volume":"28 1","pages":"28-36"},"PeriodicalIF":1.2,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000486716","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35912119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Draft Genome Sequence of Bacillus cereus CITVM-11.1, a Strain Exhibiting Interesting Antifungal Activities. 具有抗真菌活性的蜡样芽孢杆菌CITVM-11.1的基因组序列草图

IF 1.2

Journal of Molecular Microbiology and Biotechnology Pub Date : 2018-01-01 Epub Date: 2018-04-25 DOI: 10.1159/000487597

Javier Caballero, Cecilia Peralta, Antonella Molla, Eleodoro E Del Valle, Primitivo Caballero, Colin Berry, Verónica Felipe, Pablo Yaryura, Leopoldo Palma

引用次数: 5