Journal of Molecular Microbiology and Biotechnology最新文献_第2页

Vaccination of Mice with Listeria ivanovii Expressing the Truncated M Protein of Porcine Reproductive and Respiratory Syndrome Virus Induces both Antigen-Specific CD4+ and CD8+ T Cell-Mediated Immunity. 用表达猪生殖与呼吸综合征病毒M蛋白的伊万诺氏李斯特菌接种小鼠可诱导抗原特异性CD4+和CD8+ T细胞介导的免疫

IF 1.2

Journal of Molecular Microbiology and Biotechnology Pub Date : 2019-01-01 Epub Date: 2020-04-14 DOI: 10.1159/000506686

Tian Tang, Chuan Wang, Qikang Pu, Jinmei Peng, Sijing Liu, Chenyan Ren, Mingjuan Jiang, Zhijun Tian

{"title":"Vaccination of Mice with Listeria ivanovii Expressing the Truncated M Protein of Porcine Reproductive and Respiratory Syndrome Virus Induces both Antigen-Specific CD4+ and CD8+ T Cell-Mediated Immunity.","authors":"Tian Tang, Chuan Wang, Qikang Pu, Jinmei Peng, Sijing Liu, Chenyan Ren, Mingjuan Jiang, Zhijun Tian","doi":"10.1159/000506686","DOIUrl":"https://doi.org/10.1159/000506686","url":null,"abstract":"Porcine reproductive and respiratory syndrome (PRRS), a serious disease of swine caused by the PRRS virus (PRRSV), had a severe economic impact worldwide. As commonly used PRRS vaccines, the attenuated or inactivated vaccines, provide unsatisfactory immune protection, a new PRRS vaccine is urgently needed. In this study, a part of the PRRSV ORF6 gene (from 253 to 519 bp) encoding the hydrophilic domain of PRRSV M protein was integrated into two Listeria strains via homologous recombination to generate two PRRS vaccine candidates, namely LI-M' and LM-ΔactAplcB-M'. Both candidate vaccines showed similar growth rate as their parent strains in culture media, but presented different bacterial loads in target organs. As the integrated heterogenous gene was not expressed, LM-ΔactAplcB-M' was excluded from the immunological test. In a mouse model, LI-M' provoked both CD4+ and CD8+ T cell-mediated immunity. In addition, LI-M' boosting dramatically enhanced CD8+ T cell-mediated immunity without affecting the response intensity of CD4+ T cell-mediated immunity. All of these data suggest that LI-M' is a promising PRRS vaccine candidate.","PeriodicalId":16370,"journal":{"name":"Journal of Molecular Microbiology and Biotechnology","volume":"29 1-6","pages":"74-82"},"PeriodicalIF":1.2,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000506686","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37835269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Immunogenicity Evaluation of Chimeric Subunit Vaccine Comprising Adhesion Coli Surface Antigens from Enterotoxigenic Escherichia coli. 产肠毒素大肠杆菌黏附大肠杆菌表面抗原嵌合亚单位疫苗的免疫原性评价

IF 1.2

Journal of Molecular Microbiology and Biotechnology Pub Date : 2019-01-01 Epub Date: 2020-07-09 DOI: 10.1159/000509708

Dorna Khoobbakht, Shohreh Zare Karizi, Mohammad Javad Motamedi, Rouhollah Kazemi, Pooneh Roghanian, Jafar Amani

{"title":"Immunogenicity Evaluation of Chimeric Subunit Vaccine Comprising Adhesion Coli Surface Antigens from Enterotoxigenic Escherichia coli.","authors":"Dorna Khoobbakht, Shohreh Zare Karizi, Mohammad Javad Motamedi, Rouhollah Kazemi, Pooneh Roghanian, Jafar Amani","doi":"10.1159/000509708","DOIUrl":"https://doi.org/10.1159/000509708","url":null,"abstract":"Enterotoxigenic Escherichia coli (ETEC) is the most common agent of diarrhea morbidity in developing countries. ETEC adheres to host intestinal epithelial cells via various colonization factors. The CooD and CotD proteins play a significant role in bacteria binding to the intestinal epithelial cells as adhesin tip subunits of CS1 and CS2 pili. The purpose here was to design a new construction containing cooD and cotD genes and use several types of bioinformatics software to predict the structural and immunological properties of the designed antigen. The fusion gene was synthesized with codon bias of E. coli in order to increase the expression level of the protein. The amino acid sequences, protein structure, and immunogenicity properties of potential antigens were analyzed in silico. The chimeric protein was expressed in E. coliBL21 (DE3). The antigenicity of the recombinant proteins was verified by Western blotting and ELISA. In order to assess the induced immunity, the immunized mice were challenged with wild-type ETEC by an intraperitoneal route. Immunological analyses showed the production of a high titer of IgG serum with no sign of serum-mucosal IgA antibody response. The result of the challenge assay showed that 30% of immunized mice survived. The results of this study showed that CooD-CotD recombinant protein can stimulate immunity against ETEC. The designed chimera could be a prototype for the subunit vaccine, which is worthy of further consideration.","PeriodicalId":16370,"journal":{"name":"Journal of Molecular Microbiology and Biotechnology","volume":"29 1-6","pages":"91-100"},"PeriodicalIF":1.2,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000509708","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38135856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Non-Caloric Artificial Sweeteners Modulate the Expression of Key Metabolic Genes in the Omnipresent Gut Microbe Escherichia coli. 无热量人工甜味剂调节无处不在的肠道微生物大肠杆菌关键代谢基因的表达。

IF 1.2

Journal of Molecular Microbiology and Biotechnology Pub Date : 2019-01-01 Epub Date: 2019-12-18 DOI: 10.1159/000504511

Rizwan Mahmud, Saadlee Shehreen, Shayan Shahriar, Md Siddiqur Rahman, Sharif Akhteruzzaman, Abu Ashfaqur Sajib

{"title":"Non-Caloric Artificial Sweeteners Modulate the Expression of Key Metabolic Genes in the Omnipresent Gut Microbe Escherichia coli.","authors":"Rizwan Mahmud, Saadlee Shehreen, Shayan Shahriar, Md Siddiqur Rahman, Sharif Akhteruzzaman, Abu Ashfaqur Sajib","doi":"10.1159/000504511","DOIUrl":"https://doi.org/10.1159/000504511","url":null,"abstract":"The human gut is inhabited by several hundred different bacterial species. These bacteria are closely associated with our health and well-being. The composition of these diverse commensals is influenced by our dietary intakes. Non-caloric artificial sweeteners (NAS) have gained global popularity, particularly among diabetic patients, due to their perceived health benefits, such as reduction of body weight and maintenance of blood glucose level compared to caloric sugars. Recent studies have reported that these artificial sweeteners can alter the composition of gut microbiota and, thus, affect our normal physiological state. Here, we investigated the effect of aspartame and acesulfame potassium (ace-K), two popular NAS, in a commercial formulation on the growth and metabolic pathways of omnipresent gut commensal Escherichia coliby analyzing the relative expression levels of the key genes, which control over twenty important metabolic pathways. Treatment with NAS preparation (aspartame and ace-K) modulates the growth of E. colias well as inducing the expression of important metabolic genes associated with glucose (pfkA, sucA, aceE, pfkB, lpdA), nucleotide (tmk, adk, tdk, thyA), and fatty acid (fabI) metabolisms, among others. Several of the affected geneswere previously reported to be important for the colonization of the microbes in the gut. These findings may shed light on the mechanism of alteration of gut microbes and their metabolism by NAS.","PeriodicalId":16370,"journal":{"name":"Journal of Molecular Microbiology and Biotechnology","volume":"29 1-6","pages":"43-56"},"PeriodicalIF":1.2,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000504511","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37470029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Production of Mycobacterium bovis Antigens Included in Recombinant Occlusion Bodies of Baculovirus. 杆状病毒重组闭塞体含牛分枝杆菌抗原的制备。

IF 1.2

Journal of Molecular Microbiology and Biotechnology Pub Date : 2019-01-01 Epub Date: 2020-04-07 DOI: 10.1159/000506687

Luciana Villafañe, Marina Andrea Forrellad, María Gabriela López, Sergio Garbaccio, Carlos Garro, Rosana Valeria Rocha, María Emilia Eirin, Mahavir Singh, Oscar A Taboga, Fabiana Bigi

{"title":"Production of Mycobacterium bovis Antigens Included in Recombinant Occlusion Bodies of Baculovirus.","authors":"Luciana Villafañe, Marina Andrea Forrellad, María Gabriela López, Sergio Garbaccio, Carlos Garro, Rosana Valeria Rocha, María Emilia Eirin, Mahavir Singh, Oscar A Taboga, Fabiana Bigi","doi":"10.1159/000506687","DOIUrl":"https://doi.org/10.1159/000506687","url":null,"abstract":"Bovine tuberculosis (bTB) is a disease produced by Mycobacterium bovis that affects livestock, wild animals, and humans. The classical diagnostic method to detect bTB is measuring the response induced with the intradermal injection of purified protein derivative of M. bovis (PPDb). Another ancillary bTB test detects IFN-γ produced in whole blood upon stimulation with PPDb, protein/peptide cocktails, or individual antigens. Among the most used M. bovis antigens in IFN-γ assays are the secreted proteins ESAT-6 and CFP-10, which together with antigen Rv3615c improve the sensitivity of the test in comparison to PPDb. Protein reagents for immune stimulation are generally obtained from Escherichia coli, because this bacterium produces a high level of recombinant proteins. However, E. coli recombinant antigens are in general contaminated with lipopolysaccharides and other components that produce non-specific IFN-γ secretion in in vitro assays. In this work, we produced the relevant ESAT-6, CFP-10, and Rv3615c M. bovis antigens as fusions to the polyhedrin protein from the baculovirus AcMNPV. We obtained chimeric proteins effectively incorporated to the occlusion bodies and easily purified the recombinant polyhedra with no reactive contaminants. In an IFN-γ assay, these fusion proteins showed equivalent sensibility but better specificity than the same M. bovis proteins produced in E. coli.","PeriodicalId":16370,"journal":{"name":"Journal of Molecular Microbiology and Biotechnology","volume":"29 1-6","pages":"83-90"},"PeriodicalIF":1.2,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000506687","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37811819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Enhanced Fermentable Sugar Production from Enteromorpha Polysaccharides by the Crude Enzymes of Vibrio sp. H11. 利用H11弧菌粗酶提高浒苔多糖发酵产糖的研究。

IF 1.2

Journal of Molecular Microbiology and Biotechnology Pub Date : 2019-01-01 Epub Date: 2020-03-06 DOI: 10.1159/000505371

Jin Li, Yan Xu, Tao Peng, Mingqi Zhong, Zhong Hu

{"title":"Enhanced Fermentable Sugar Production from Enteromorpha Polysaccharides by the Crude Enzymes of Vibrio sp. H11.","authors":"Jin Li, Yan Xu, Tao Peng, Mingqi Zhong, Zhong Hu","doi":"10.1159/000505371","DOIUrl":"https://doi.org/10.1159/000505371","url":null,"abstract":"In recent years, large-scale outbreaks of the green alga Enteromorpha prolifera in China's offshore waters have posed a serious threat. This study aimed to improve Enteromorpha polysaccharide (EP) enzymatic sugar production using the hydrolase system of Vibrio sp. H11, an EP-utilizing microbial strain. Strain H11 was found to contain 711 carbohydrate-related genes, and 259 genes belong to glycoside hydrolases that have the potential to hydrolyze EP. To maximize the capability of strain H11 to hydrolyze EP, both the culture medium and the composition were optimized. Response surface methodology analysis showed that maximal enzymatic production from strain H11 was 8.43 U/mL after 26-h incubation. When 50 g/L of EP were treated with crude H11 enzyme, the concentration of fermentation sugars increased by 36.12%. Under these conditions, the hydrolysates were capable of generating 3,217 mL/L of biogas and 6.74 g/L of biosolvents, with increases of 28.17 and 7.29%, respectively, compared to controls. The combined application of the H11 enzymatic system and anaerobic fermentation has the potential to improve the comprehensive application of EP.","PeriodicalId":16370,"journal":{"name":"Journal of Molecular Microbiology and Biotechnology","volume":"29 1-6","pages":"66-73"},"PeriodicalIF":1.2,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000505371","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37714877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Characterization of Alanine Dehydrogenase and Its Effect on Streptomyces coelicolorA3(2) Development in Liquid Culture. 丙氨酸脱氢酶的特性及其对冷色链霉菌a3(2)液体培养发育的影响

IF 1.2

Journal of Molecular Microbiology and Biotechnology Pub Date : 2019-01-01 Epub Date: 2019-12-18 DOI: 10.1159/000504709

Arie Van Wieren, Ryan Cook, Sudipta Majumdar

{"title":"Characterization of Alanine Dehydrogenase and Its Effect on Streptomyces coelicolorA3(2) Development in Liquid Culture.","authors":"Arie Van Wieren, Ryan Cook, Sudipta Majumdar","doi":"10.1159/000504709","DOIUrl":"https://doi.org/10.1159/000504709","url":null,"abstract":"Streptomyces, the most important group of industrial microorganisms, is harvested in liquid cultures for the production of two-thirds of all clinically relevant secondary metabolites. It is demonstrated here that the growth of Streptomyces coelicolor A3(2) is impacted by the deletion of the alanine dehydrogenase (ALD), an essential enzyme that plays a central role in the carbon and nitrogen metabolism. A long lag-phase growth followed by a slow exponential growth of S. coelicolor due to ALD gene deletion was observed in liquid yeast extract mineral salt culture. The slow lag-phase growth was replaced by the normal wild-type like growth by ALD complementation engineering. The ALD enzyme from S. coelicolor was also heterologously cloned and expressed in Escherichia coli for characterization. The optimum enzyme activity for the oxidative deamination reaction was found at 30°C, pH 9.5 with a catalytic efficiency, kcat/KM, of 2.0 ± 0.1 mM-1 s-1. The optimum enzyme activity for the reductive amination reaction was found at 30°C, pH 9.0 with a catalytic efficiency, kcat/KM, of 1.9 ± 0.1 mM-1 s-1.","PeriodicalId":16370,"journal":{"name":"Journal of Molecular Microbiology and Biotechnology","volume":"29 1-6","pages":"57-65"},"PeriodicalIF":1.2,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000504709","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37470031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Front & Back Matter 正面和背面事项

IF 1.2

Journal of Molecular Microbiology and Biotechnology Pub Date : 2018-05-01 DOI: 10.1159/000489971

引用次数: 0

Contents 内容

IF 1.2

Journal of Molecular Microbiology and Biotechnology Pub Date : 2018-02-01 DOI: 10.1159/000487342

引用次数: 0

Overexpression of an Inulinase Gene in an Oleaginous Yeast, Aureobasidium melanogenum P10, for Efficient Lipid Production from Inulin. 产油酵母黑素小孢子酵母P10中菊粉酶基因的过表达及其高效产脂作用。

IF 1.2

Journal of Molecular Microbiology and Biotechnology Pub Date : 2018-01-01 Epub Date: 2019-01-03 DOI: 10.1159/000493139

Yan-Feng Li, Hong Jiang, Zhong Hu, Guang-Lei Liu, Zhen-Ming Chi, Zhe Chi

引用次数: 4

Improvement of Spinosad Production upon Utilization of Oils and Manipulation of β-Oxidation in a High-Producing Saccharopolyspora spinosa Strain. 利用油脂提高棘糖多糖产量及调控高产棘糖多孢子菌β-氧化。

IF 1.2

Journal of Molecular Microbiology and Biotechnology Pub Date : 2018-01-01 Epub Date: 2018-05-04 DOI: 10.1159/000487854

Ying Huang, Xiaolin Zhang, Chen Zhao, Xuhui Zhuang, Lin Zhu, Chao Guo, Yuan Song

{"title":"Improvement of Spinosad Production upon Utilization of Oils and Manipulation of β-Oxidation in a High-Producing Saccharopolyspora spinosa Strain.","authors":"Ying Huang, Xiaolin Zhang, Chen Zhao, Xuhui Zhuang, Lin Zhu, Chao Guo, Yuan Song","doi":"10.1159/000487854","DOIUrl":"https://doi.org/10.1159/000487854","url":null,"abstract":"Spinosad, a member of polyketide-derived macrolides produced in the actinomycete Saccharopolyspora spinosa, has been developed as a broad-spectrum and effective insecticide. The β-oxidation pathway could be an important source of building blocks for the biosynthesis of spinosad, thus the effect of vegetable oils on the production of spinosad in a high-yield strain was investigated. The spinosad production increased significantly with the addition of strawberry seed oil (511.64 mg/L) and camellia oil (520.07 mg/L) compared to the control group without oil (285.76 mg/L) and soybean oil group (398.11 mg/L). It also revealed that the addition of oils would affect the expression of genes involved in fatty acid metabolism, precursor supply, and oxidative stress. The genetically engineered strain, in which fadD1 and fadE genes of Streptomyces coelicolor were inserted, produced spinosad up to 784.72 mg/L in the medium containing camellia oil, while a higher spinosad production level (843.40 mg/L) was detected with the addition of 0.01 mM of thiourea.","PeriodicalId":16370,"journal":{"name":"Journal of Molecular Microbiology and Biotechnology","volume":"28 2","pages":"53-64"},"PeriodicalIF":1.2,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000487854","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36072541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5