利用油脂提高棘糖多糖产量及调控高产棘糖多孢子菌β-氧化。

IF 1.2 Q2 Biochemistry, Genetics and Molecular Biology
Ying Huang, Xiaolin Zhang, Chen Zhao, Xuhui Zhuang, Lin Zhu, Chao Guo, Yuan Song
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引用次数: 5

摘要

Spinosad是一种广谱有效的杀虫剂,是由放线菌Saccharopolyspora spinosa产生的聚酮衍生大环内酯类化合物。β-氧化途径可能是spinosad生物合成的重要组成部分,因此研究了植物油对高产菌株spinosad生产的影响。添加草莓籽油(511.64 mg/L)和茶油(520.07 mg/L)显著高于不添加油的对照组(285.76 mg/L)和大豆油组(398.11 mg/L)。研究还发现,添加油脂会影响脂肪酸代谢、前体供应和氧化应激相关基因的表达。在含有油茶油的培养基中,插入彩色链霉菌fadD1和fadE基因的基因工程菌株的产糖量最高可达784.72 mg/L,添加0.01 mM硫脲的产糖量最高可达843.40 mg/L。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Improvement of Spinosad Production upon Utilization of Oils and Manipulation of β-Oxidation in a High-Producing Saccharopolyspora spinosa Strain.

Spinosad, a member of polyketide-derived macrolides produced in the actinomycete Saccharopolyspora spinosa, has been developed as a broad-spectrum and effective insecticide. The β-oxidation pathway could be an important source of building blocks for the biosynthesis of spinosad, thus the effect of vegetable oils on the production of spinosad in a high-yield strain was investigated. The spinosad production increased significantly with the addition of strawberry seed oil (511.64 mg/L) and camellia oil (520.07 mg/L) compared to the control group without oil (285.76 mg/L) and soybean oil group (398.11 mg/L). It also revealed that the addition of oils would affect the expression of genes involved in fatty acid metabolism, precursor supply, and oxidative stress. The genetically engineered strain, in which fadD1 and fadE genes of Streptomyces coelicolor were inserted, produced spinosad up to 784.72 mg/L in the medium containing camellia oil, while a higher spinosad production level (843.40 mg/L) was detected with the addition of 0.01 mM of thiourea.

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来源期刊
Journal of Molecular Microbiology and Biotechnology
Journal of Molecular Microbiology and Biotechnology 生物-生物工程与应用微生物
CiteScore
3.90
自引率
0.00%
发文量
0
审稿时长
>12 weeks
期刊介绍: We are entering a new and exciting era of microbiological study and application. Recent advances in the now established disciplines of genomics, proteomics and bioinformatics, together with extensive cooperation between academic and industrial concerns have brought about an integration of basic and applied microbiology as never before.
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