Journal of Liquid Chromatography & Related Technologies最新文献_第6页

Integrated methods for fingerprinting of monofloral honeys 单花蜂蜜指纹图谱的综合方法

IF 1.3 4区化学

Journal of Liquid Chromatography & Related Technologies Pub Date : 2023-06-22 DOI: 10.1080/10826076.2023.2224024

Alina Tarna, C. Cimpoiu, A. Hosu

{"title":"Integrated methods for fingerprinting of monofloral honeys","authors":"Alina Tarna, C. Cimpoiu, A. Hosu","doi":"10.1080/10826076.2023.2224024","DOIUrl":"https://doi.org/10.1080/10826076.2023.2224024","url":null,"abstract":"Abstract Honey is an important functional food due to its content of polyphenolic compounds with beneficial effects on human body. The analysis of polyphenolic compound in honey is very promising in determination of floral origin and so for quality control. This study approaches a new methodology using integrated methods for the determination of honey authenticity. Polyphenolic compounds from seven different types of monofloral honey from two different sources were extracted and analyzed by thin layer chromatography (TLC) and UV-Vis spectroscopy. Chromatographic analysis was performed on TLC Silica gel 60 RP-18 F254 plates using a mobile phase consisting in a mixture of methanol: water: formic acid (5: 5: 0.1, v/v/v). The images from the TLC analysis were processed and the obtained chromatograms together with the UV-Vis deconvoluted spectra of honey were subjected to visual and statistical analysis. The obtained results showed notable differences between the honey samples, which can be used to establish their floral origin and for their authentication. The proposed methodology is easy to perform, cost-effective and offers complementary information, therefore it has the potential of being used for the honey authentication. GRAPHICAL ABSTRACT","PeriodicalId":16295,"journal":{"name":"Journal of Liquid Chromatography & Related Technologies","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49038966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Library-based UHPLC-Q-exactive-orbitrap-MS putative identification of isomeric and non-isomeric bioactives from Zibushengfa Tablet and pharmacopeia quality-marker chemistry 基于uhplc - q -轨道谱-质谱的子补生发片异构体和非异构体生物活性推定鉴定及药典质量标记化学

IF 1.3 4区化学

Journal of Liquid Chromatography & Related Technologies Pub Date : 2023-06-21 DOI: 10.1080/10826076.2023.2223640

Shaoman Chen, Xican Li, Jingyuan Zeng, Rongxin Cai, Chunhou Li, Chuanbing Chen

{"title":"Library-based UHPLC-Q-exactive-orbitrap-MS putative identification of isomeric and non-isomeric bioactives from Zibushengfa Tablet and pharmacopeia quality-marker chemistry","authors":"Shaoman Chen, Xican Li, Jingyuan Zeng, Rongxin Cai, Chunhou Li, Chuanbing Chen","doi":"10.1080/10826076.2023.2223640","DOIUrl":"https://doi.org/10.1080/10826076.2023.2223640","url":null,"abstract":"Abstract Zibushengfa Tablet is a widely consumed traditional Chinese medicine (TCM) prescription. However, its main bioactives remain unclear and its pharmacopeia quality-marker (Q-marker) is poorly characterized nowadays. The present study analyzed its lyophilized aqueous powder using ultra-high-performance liquid chromatography-quadrupole-orbitrap mass spectrometry. Through comparison with authentic standards in the library, 46 bioactives were putatively identified, including quinic acid, D-gluconic acid, gallic acid, protocatechuic acid, 5-caffeoylquinic acid, L-tryptophan, salidroside, (+)-catechin hydrate, caffeic acid, cryptochlorogenic acid, mangiferin, ferulic acid, myricetin-3-O-galactoside, 2,3,5,4’-tetrahydroxystilbene-2-O-glucoside, forsythoside B, myricitrin, rutin, hyperoside, acteoside, 3,4-dicaffeoylquinic acid, myricetin, senkyunolide A, isochlorogenic acid C, quercitrin, (+)-epipinoresinol, quercetin, biochanin A, calycosin, luteolin, wedelolactone, calycosin-7-O-glucoside, chrysoeriol, trans-cinnamic acid, formononetin, chrysin, 3,3’,4’,5,6,7,8-heptamethoxyflavone, Z-ligustilide, amentoflavone, tangeretin, hinokiflavone, emodin, physcion, linoleic acid, stearic acid, palmitic acid, and palmitic acid ethyl ester. Of these, four pairs of isomers were successfully differentiated using the newly established library-filtering strategy. On this basis, senkyunolide A, amentoflavone, quercetin, quercitrin, and wedelolactone are preliminarily suggested as additional quality-markers for the consideration by Pharmacopeia Committee, to prevent the possible adulterate. Graphical Abstract","PeriodicalId":16295,"journal":{"name":"Journal of Liquid Chromatography & Related Technologies","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41262260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Advances on analysis methods of ginkgo biloba leaf extract 银杏叶提取物分析方法研究进展

IF 1.3 4区化学

Journal of Liquid Chromatography & Related Technologies Pub Date : 2023-06-21 DOI: 10.1080/10826076.2023.2223618

Jinhao Li, Jing Lan, Shufang Wang, Jianbiao Yao, Fangfang Wang, Xingchu Gong

引用次数: 0

Development of UPLC-MS/MS methods for quantitation of glutathione, cysteine, 14,15-epoxyeicosatrienoic acid, and 15-hydroxyeicosatetraenoic acid and application in paraquat poisoning cells 谷胱甘肽、半胱氨酸、14,15-环氧二十碳三烯酸和15-羟基二十碳四烯酸的UPLC-MS/MS定量方法的建立及其在百草枯中毒细胞中的应用

IF 1.3 4区化学

Journal of Liquid Chromatography & Related Technologies Pub Date : 2023-06-15 DOI: 10.1080/10826076.2023.2223634

Cong-Rong Tang, Xingjie Zhu, Zheng Yu, Xiaofan Ke, Jianhui Yang, Zhongqiu Lu, Lufeng Hu

{"title":"Development of UPLC-MS/MS methods for quantitation of glutathione, cysteine, 14,15-epoxyeicosatrienoic acid, and 15-hydroxyeicosatetraenoic acid and application in paraquat poisoning cells","authors":"Cong-Rong Tang, Xingjie Zhu, Zheng Yu, Xiaofan Ke, Jianhui Yang, Zhongqiu Lu, Lufeng Hu","doi":"10.1080/10826076.2023.2223634","DOIUrl":"https://doi.org/10.1080/10826076.2023.2223634","url":null,"abstract":"Abstract Glutathione (GSH) and cysteine are important antioxidants in maintaining the oxidation-reduction balance. 14,15-epoxyeicosatrienoic acid (14,15-EET) and 15-hydroxyeicosatetraenoic acid (15-HETE) are biological active compounds metabolized from arachidonic acid. To dynamically monitor GSH, cysteine, 14,15-EET, and 15-HETE, an ultra-high-performance liquid chromatography-mass spectrometry (UPLC-MS/MS) method was developed. The derivative GSH, cysteine, and underivatized 14,15-EET, 15-HETE were determined by electrospray ionization source with multiple reaction monitoring. The developed method was well validated and applied to monitor the extracellular concentration of GSH, cysteine, 14,15-EET, and 15-HETE in paraquat (PQ) poisoning LO2 cells. It was shown that the derivation of GSH and cysteine is complete and specific. There was a good linear relationship among GSH, cysteine, 14,15-EET, and 15-HETE. The relative standard deviations of precision and accuracy were all within 15%. After PQ poisoning, the extracellular concentrations of GSH and cysteine were increased at 72 h. 14,15-EET and 15-HETE started to increase at 24 h, earlier than GSH and cysteine. Irreversible pathological alterations appeared at 48 h. In conclusion, a fast and specific UPLC-MS/MS method for the determination of GSH, cysteine, 14,15-EET, and 15-HETE has been developed and validated. PQ induced the increase of 14,15-EET and 15-HETE earlier than that of GSH and cysteine in LO2 cells culture medium. Graphical Abstract","PeriodicalId":16295,"journal":{"name":"Journal of Liquid Chromatography & Related Technologies","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48406216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative evaluation of solid-phase extraction and in-tube liquid-liquid extraction for the determination of Organophosphorus pesticides in the surface water from Medjerda River, Tunisia 固相萃取法和管内液-液萃取法测定突尼斯Medjerda河地表水中有机磷农药的比较评价

IF 1.3 4区化学

Journal of Liquid Chromatography & Related Technologies Pub Date : 2023-06-10 DOI: 10.1080/10826076.2023.2222301

Mouna Necibi, N. Mzoughi

{"title":"Comparative evaluation of solid-phase extraction and in-tube liquid-liquid extraction for the determination of Organophosphorus pesticides in the surface water from Medjerda River, Tunisia","authors":"Mouna Necibi, N. Mzoughi","doi":"10.1080/10826076.2023.2222301","DOIUrl":"https://doi.org/10.1080/10826076.2023.2222301","url":null,"abstract":"Abstract In the present work, we develop, validate, and compare two extraction procedures (in-tube liquid-liquid extraction (ITLLE) and solid-phase extraction (SPE)). The parameters that influence extraction efficiency, the nature and volume of the extraction solvent, as well as the pH nature, were improved and a standard solution of Organophosphorus pesticides (OPPs) was used. Results indicated that SPE is clearly more successful than ITLLE extraction under optimum conditions. The validated method showed that this approach had good linearity, with correlation coefficients ranging from 0.991 to 0.998 and low detection and quantification limits of 0.01–0.03 ng L−1 and 0.03–0.9 ng L−1, respectively. Relative standard deviations were in the range of 0.2 and 1.2%, while extraction recoveries varied from 79 to 97%. The SPE was selected and, optimum recoveries for OPPs were obtained at pH 7, with 10 mL of ethyl acetate. The suggested approach was effectively applied to the analysis of water samples collected from the Medjerda River in two seasons. The average OPPs concentrations ranged from not detected (ND) to 17.5 ng L−1 during the dry season and from not detected ND to 13.1 ng L−1 during the wet season. The results demonstrate that none of the OPPs concentrations measured exceeded the regulatory limits. Graphical Abstract","PeriodicalId":16295,"journal":{"name":"Journal of Liquid Chromatography & Related Technologies","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49503596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Uncovering the Q-marker and potential mechanisms of Qishan Formula for lung cancer based on multi-technology 基于多技术的肺癌芪山方q标记物及其潜在机制研究

IF 1.3 4区化学

Journal of Liquid Chromatography & Related Technologies Pub Date : 2023-05-31 DOI: 10.1080/10826076.2023.2216765

Yutong Sui, Yanqun Yang, Jing Zhang, Ziwei Wang, Xue Geng, Jiakang Jiang

{"title":"Uncovering the Q-marker and potential mechanisms of Qishan Formula for lung cancer based on multi-technology","authors":"Yutong Sui, Yanqun Yang, Jing Zhang, Ziwei Wang, Xue Geng, Jiakang Jiang","doi":"10.1080/10826076.2023.2216765","DOIUrl":"https://doi.org/10.1080/10826076.2023.2216765","url":null,"abstract":"Abstract Qishan Formula (QSF) is mainly used to treat lung cancer and its side effects of radiotherapy and chemotherapy. Although the clinical efficacy of QSF has been recognized, its mechanism of action and active ingredients are still unclear. This study aims to explore the potential Q-Marker of QSF and reveal its anti-lung cancer mechanism. Firstly, the fingerprints of 10 batches of QSF were established, and the potential Q-markers were screened by chemometrics, then network pharmacology and molecular docking methods were used to explore its anti-lung cancer mechanism. Finally, the potential Q-markers were quantitatively by UPLC-QDA. The results showed that 48 common peaks were identified by the established fingerprint, and calycosin, ginsenoside Rg1, atractylenolide III, atractylenolide I and ginsenoside Rb1 were screened as potential Q-Markers of QSF by chemometrics. Network pharmacology and molecular docking found that the potential Q-Markers may regulate various signaling pathways through targets such as FOS, IL6, EGFR, ESR1, MAPK1, and MAPK3, and play a therapeutic role in lung cancer. Besides, the established UPLC-QDA can also perform quality control for Q-Markers of QSF. This study can provide academic ideas and lay a foundation for the comprehensive quality control and mechanism research of QSF and other compound preparation. Graphical Abstract","PeriodicalId":16295,"journal":{"name":"Journal of Liquid Chromatography & Related Technologies","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47454031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Holistic chemical characterization of Zaoren Anshen preparations based on HPLC fingerprint and multi-components quantification 基于高效液相色谱指纹图谱和多组分定量的皂仁安神制剂整体化学表征

IF 1.3 4区化学

Journal of Liquid Chromatography & Related Technologies Pub Date : 2023-03-16 DOI: 10.1080/10826076.2023.2206667

Man-Jiang Xie, Jie Wang, Chenguang Dong, P. Tu, Qingying Zhang

引用次数: 0

Differential compounds of licorice before and after honey roasted and anti-arrhythmia mechanism via LC-MS/MS and network pharmacology analysis LC-MS/MS和网络药理学分析蜂蜜烘烤前后甘草的差异化合物及其抗心律失常机制

IF 1.3 4区化学

Journal of Liquid Chromatography & Related Technologies Pub Date : 2023-03-16 DOI: 10.1080/10826076.2023.2202238

Lujie Lu, Wen-xin Wang, Peijun Sun, Shuwei Yan, Hui Chen, Xiao Liu, Jiajia Dong, Lihong Chen, T. Lu

{"title":"Differential compounds of licorice before and after honey roasted and anti-arrhythmia mechanism via LC-MS/MS and network pharmacology analysis","authors":"Lujie Lu, Wen-xin Wang, Peijun Sun, Shuwei Yan, Hui Chen, Xiao Liu, Jiajia Dong, Lihong Chen, T. Lu","doi":"10.1080/10826076.2023.2202238","DOIUrl":"https://doi.org/10.1080/10826076.2023.2202238","url":null,"abstract":"Abstract Licorice is mainly used to treat cough in clinics. After being roasted with honey, licorice has a cardiac protective effect. Due to its different sources, the chemical components in licorice are also diverse. To distinguish the different compounds between three sources of raw licorice (RL) and between three sources of honey-roasted licorice (HRL), a UPLC-QTOF-MS/MS method was established. Eighteen batches of samples were analyzed which were derived from Glycyrrhiza uralensis Fisch., Glycyrrhiza inflata Bat., and Glycyrrhiza glabra L. In addition, the potential key pathways of HRL to anti-arrhythmia were explored by network pharmacology. Among the three sources of RL, 77 differential compounds were detected in the positive ion mode; and 62 differential compounds were detected in the negative ion mode, of which 21 differential compounds were detected in both ion modes. Twenty-nine differential compounds were identified before and after honey-roasted of G. uralensis Fisch., 51 and 17 differential compounds were identified in G. glabra L. and G. inflata Bat., receptively. The network pharmacological analysis predicted that Rap1, Ras, PI3K-Akt, and cAMP signaling pathway might be closely related to the anti-arrhythmia mechanism of HRL. This study provides a theoretical reference for the quality improvement and clinical application of licorice. Graphical Abstract","PeriodicalId":16295,"journal":{"name":"Journal of Liquid Chromatography & Related Technologies","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47155385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comprehensive review on QbD driven analytical procedures developed for the analysis of various drugs 全面回顾了QbD驱动的分析方法，用于分析各种药物

IF 1.3 4区化学

Journal of Liquid Chromatography & Related Technologies Pub Date : 2023-03-16 DOI: 10.1080/10826076.2023.2204238

Aggarapu Susmitha, G. Rajitha, Gireesh Kumar Eri

引用次数: 0

Hydrophilic molecularly imprinted chitosan microspheres based on deep eutectic solvents for micro-solid phase extraction of catechin 基于深共晶溶剂的亲水分子印迹壳聚糖微球微固相萃取儿茶素

IF 1.3 4区化学

Journal of Liquid Chromatography & Related Technologies Pub Date : 2023-03-16 DOI: 10.1080/10826076.2023.2208201

Mengdi Yuan, Chenglin Zhong, K. Row, Guizhen Li

引用次数: 0