Journal of Lipids最新文献

{"title":"Lipids and the Permeability and Antimicrobial Barriers of the Skin.","authors":"Philip W Wertz","doi":"10.1155/2018/5954034","DOIUrl":"https://doi.org/10.1155/2018/5954034","url":null,"abstract":"<p><p>The primary purpose of the epidermis of terrestrial vertebrates is to produce the stratum corneum, which serves as the interface between the organism and the environment. As such, the stratum corneum provides a permeability barrier which both limits water loss through the skin and provides a relatively tough permeability barrier. This provides for a degree of resistance to mechanical trauma and prevents or limits penetration of potentially harmful substances from the environment. The stratum corneum consists of an array of keratinized cells embedded in a lipid matrix. It is this intercellular lipid that determines the permeability of the stratum corneum. The main lipids here are ceramides, cholesterol, and fatty acids. In addition, the skin surface of mammals, including humans, is coated by a lipid film produced by sebaceous glands in the dermis and secreted through the follicles. Human sebum consists mainly of squalene, wax monoesters, and triglycerides with small proportions of cholesterol and cholesterol esters. As sebum passes through the follicles, some of the triglycerides are hydrolyzed by bacteria to liberate free fatty acids. Likewise, near the skin surface, where water becomes available, some of the ceramides are acted upon by an epithelial ceramidase to liberate sphingosine, dihydrosphingosine, and 6-hydroxysphingosine. Some of the free fatty acids, specifically lauric acid and sapienic acid, have been shown to have antibacterial, antifungal, and antiviral activity. Also, the long-chain bases have broad spectrum antibacterial activity.</p>","PeriodicalId":16274,"journal":{"name":"Journal of Lipids","volume":"2018 ","pages":"5954034"},"PeriodicalIF":5.3,"publicationDate":"2018-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2018/5954034","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36518783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitochondrial-Targeted Antioxidant MitoQ Prevents <i>E. coli</i> Lipopolysaccharide-Induced Accumulation of Triacylglycerol and Lipid Droplets Biogenesis in Epithelial Cells.","authors":"Ekaterina Fock,&nbsp;Vera Bachteeva,&nbsp;Elena Lavrova,&nbsp;Rimma Parnova","doi":"10.1155/2018/5745790","DOIUrl":"https://doi.org/10.1155/2018/5745790","url":null,"abstract":"<p><p>The effect of bacterial lipopolysaccharide (LPS) on eukaryotic cell could be accompanied by a significant metabolic shift that includes accumulation of triacylglycerol (TAG) in lipid droplets (LD), ubiquitous organelles associated with fatty acid storage, energy regulation and demonstrated tight spatial and functional connections with mitochondria. The impairment of mitochondrial activity under pathological stimuli has been shown to provoke TAG storage and LD biogenesis. However the potential mechanisms that link mitochondrial disturbances and TAG accumulation are not completely understood. We hypothesize that mitochondrial ROS (mROS) may play a role of a trigger leading to subsequent accumulation of intracellular TAG and LD in response to a bacterial stimulus. Using isolated epithelial cells from the frog urinary bladder, we showed that LPS decreased fatty acids oxidation, enhanced TAG deposition, and promoted LD formation. LPS treatment did not affect the mitochondrial membrane potential but increased cellular ROS production and led to impairment of mitochondrial function as revealed by decreased ATP production and a reduced maximal oxygen consumption rate (OCR) and OCR directed at ATP turnover. The mitochondrial-targeted antioxidant MitoQ at a dose of 25 nM did not prevent LPS-induced alterations in cellular respiration, but, in contrast to nonmitochondrial antioxidant <i>α</i>-tocopherol, reduced the effect of LPS on the generation of ROS, restored the LPS-induced decline of fatty acids oxidation, and prevented accumulation of TAG and LD biogenesis. The data obtained indicate the key signaling role of mROS in the lipid metabolic shift that occurs under the impact of a bacterial pathogen in epithelial cells.</p>","PeriodicalId":16274,"journal":{"name":"Journal of Lipids","volume":"2018 ","pages":"5745790"},"PeriodicalIF":5.3,"publicationDate":"2018-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2018/5745790","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36518782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Free Radical Scavenging Capacity, Carotenoid Content, and NMR Characterization of <i>Blighia sapida</i> Aril Oil.","authors":"Andrea Goldson Barnaby,&nbsp;Jesse Clarke,&nbsp;Dane Warren,&nbsp;Kailesha Duffus","doi":"10.1155/2018/1762342","DOIUrl":"https://doi.org/10.1155/2018/1762342","url":null,"abstract":"<p><p><i>Blighia sapida</i> aril oil is rich in monounsaturated fatty acids but is however currently not utilized industrially. The oil was characterized utilizing nuclear magnetic resonance (NMR) and Fourier Transform Infrared Spectroscopy (FTIR). A spectrophotometric assay was conducted to determine the free radical scavenging properties and carotenoid content of the oil. Chemical shifts resonating between <i>δ</i> 5.30 and 5.32 in the ¹H NMR are indicative of olefinic protons present in ackee aril oil which are due to the presence of oleic acid. A peak at 3006 cm^-1 in the FTIR spectra confirms the high levels of monounsaturation. The oil has a free radical scavenging activity of 48%  ± 2.8% and carotenoid content of 21 ± 0.2 ppm.</p>","PeriodicalId":16274,"journal":{"name":"Journal of Lipids","volume":"2018 ","pages":"1762342"},"PeriodicalIF":5.3,"publicationDate":"2018-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2018/1762342","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36466523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intracellular and Plasma Membrane Events in Cholesterol Transport and Homeostasis.","authors":"Dmitry Y Litvinov,&nbsp;Eugeny V Savushkin,&nbsp;Alexander D Dergunov","doi":"10.1155/2018/3965054","DOIUrl":"https://doi.org/10.1155/2018/3965054","url":null,"abstract":"<p><p>Cholesterol transport between intracellular compartments proceeds by both energy- and non-energy-dependent processes. Energy-dependent vesicular traffic partly contributes to cholesterol flux between endoplasmic reticulum, plasma membrane, and endocytic vesicles. Membrane contact sites and lipid transfer proteins are involved in nonvesicular lipid traffic. Only \"active\" cholesterol molecules outside of cholesterol-rich regions and partially exposed in water phase are able to fast transfer. The dissociation of partially exposed cholesterol molecules in water determines the rate of passive aqueous diffusion of cholesterol out of plasma membrane. ATP hydrolysis with concomitant conformational transition is required to cholesterol efflux by ABCA1 and ABCG1 transporters. Besides, scavenger receptor SR-B1 is involved also in cholesterol efflux by facilitated diffusion via hydrophobic tunnel within the molecule. Direct interaction of ABCA1 with apolipoprotein A-I (apoA-I) or apoA-I binding to high capacity binding sites in plasma membrane is important in cholesterol escape to free apoA-I. ABCG1-mediated efflux to fully lipidated apoA-I within high density lipoprotein particle proceeds more likely through the increase of \"active\" cholesterol level. Putative cholesterol-binding linear motifs within the structure of all three proteins ABCA1, ABCG1, and SR-B1 are suggested to contribute to the binding and transfer of cholesterol molecules from cytoplasmic to outer leaflets of lipid bilayer. Together, plasma membrane events and intracellular cholesterol metabolism and traffic determine the capacity of the cell for cholesterol efflux.</p>","PeriodicalId":16274,"journal":{"name":"Journal of Lipids","volume":"2018 ","pages":"3965054"},"PeriodicalIF":5.3,"publicationDate":"2018-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2018/3965054","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36451965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Fast and Simple Qualitative Method for Screening Oleaginous Yeasts on Agar.","authors":"Xochitl Niehus,&nbsp;Leticia Casas-Godoy,&nbsp;Marcos Vargas-Sánchez,&nbsp;Georgina Sandoval","doi":"10.1155/2018/5325804","DOIUrl":"https://doi.org/10.1155/2018/5325804","url":null,"abstract":"<p><p>Finding new oleaginous yeasts is of great interest due to their many important applications. Currently available screening procedures are time-consuming, and most of these require liquid cultures. In this work, a new, fast, economical, and simple qualitative method for screening oleaginous yeasts was developed. The fluorescent dye, Rhodamine B, was selected because its fluorescence is directly correlated to lipid content, and no additional steps or special equipment are needed. This method only requires growing the yeasts on dyed agar plates. Under visible light, it is easy to observe that nonpigmented oleaginous yeasts become colored, whereas non-oleaginous yeasts remain uncolored. The developed method is also useful for improving medium composition in specific applications. Moreover, it was also adapted to use alternative carbon sources, such as lignocellulosic materials and glycerol. The developed method was applied to screen 124 recently isolated nonpigmented yeasts on three different carbon sources, namely, glucose, glycerol, and agave bagasse hydrolysate. Five strains were selected as good lipid producers on all tested carbon sources and accumulated over 48% lipids. Furthermore, the assay was adapted to screen reddish-pigmented yeasts. Considering all the above, the developed method has a wide range of applications in the field of microbial oils.</p>","PeriodicalId":16274,"journal":{"name":"Journal of Lipids","volume":"2018 ","pages":"5325804"},"PeriodicalIF":5.3,"publicationDate":"2018-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2018/5325804","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36434925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Proprotein Convertase Subtilisin/Kexin Type 9 Inhibitors on Nonfasting Remnant Cholesterol in a Real World Population.","authors":"Anthony P Morise,&nbsp;Jennifer Tennant,&nbsp;Sari D Holmes,&nbsp;Danyel H Tacker","doi":"10.1155/2018/9194736","DOIUrl":"https://doi.org/10.1155/2018/9194736","url":null,"abstract":"<p><strong>Background: </strong>Proprotein convertase subtilisin/kexin type 9 (PCSK9) inhibitors have demonstrated significant effects on low-density lipoprotein (LDL) cholesterol and nonhigh density lipoprotein (HDL) cholesterol. To date, there have been limited reports on the effect of PCSK9 inhibitors on remnant cholesterol.</p><p><strong>Objectives: </strong>Assess the effect of PCSK9 inhibitors on nonfasting remnant cholesterol in a real world population. Identify whether pretreatment triglyceride levels are associated with PCSK9 inhibition success as indicated by changes in remnant cholesterol levels.</p><p><strong>Methods: </strong>Patients in our adult lipid clinic (<i>n</i> = 109) receiving PCSK9 inhibition for atherosclerotic cardiovascular disease or familial hypercholesterolemia who had available pre- and post-PCSK9 inhibition standard nonfasting lipid data were, retrospectively, selected for data analysis. Remnant cholesterol was the difference between non-HDL and LDL cholesterol. LDL cholesterol was measured directly and calculated from Friedewald and Martin/Hopkins methods. Data were analyzed using repeated measures ANOVA and multivariable linear regression for differential effects on remnant and LDL cholesterol based upon pretreatment nonfasting triglyceride levels.</p><p><strong>Results: </strong>Remnant cholesterol as well as total, LDL, non-HDL cholesterol, and triglycerides decreased significantly (<i>P</i><0.001) after PCSK9 inhibition. Patients with higher pretreatment triglyceride levels showed greater decrease in remnant cholesterol after PCSK9 inhibition (<i>P</i><0.001) than those with lower pretreatment triglycerides.</p><p><strong>Conclusions: </strong>In patients receiving PCSK9 inhibitors, remnant cholesterol as determined from nonfasting blood was reduced in proportion to pretreatment triglycerides.</p>","PeriodicalId":16274,"journal":{"name":"Journal of Lipids","volume":"2018 ","pages":"9194736"},"PeriodicalIF":5.3,"publicationDate":"2018-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2018/9194736","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36393570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of <i>Helicobacter pylori</i> Infection on Serum Lipid Profile.","authors":"Mohamadreza Haeri,&nbsp;Mahmoud Parham,&nbsp;Neda Habibi,&nbsp;Jamshid Vafaeimanesh","doi":"10.1155/2018/6734809","DOIUrl":"https://doi.org/10.1155/2018/6734809","url":null,"abstract":"<p><strong>Background: </strong>Some studies suggest a significant relationship between <i>Helicobacter pylori</i> infection and atherogenesis; but the mechanism of the relationship is almost unknown. The current study aimed at evaluating the relationship between <i>H. pylori</i> infection and serum lipid profile.</p><p><strong>Patients and methods: </strong>The current study was conducted on 2573 patients, from 2008 to 2015. The serum anti-<i>Helicobacter pylori</i> antibody titer and serum lipid profile were assessed in the study population; data were statistically analyzed by SPSS version 16. <i>P</i> values < 0.05 were considered significant.</p><p><strong>Results: </strong>In the current study, 66.5% of the cases were serologically positive for <i>H. pylori</i>. Among male cases, the level of low density lipoprotein (LDL) was higher in patients with <i>H. pylori</i> infection, compared with that of the ones without the infection (<i>P</i> = 0.03); although level of triglyceride (TG) was higher and the level of high density lipoprotein (HDL) was lower in the cases with <i>H. pylori</i> infection; there was no statistically significant difference between the cases with and without <i>H. pylori</i> infection regarding the level of HDL and TG. Among female cases, the level of TG was significantly lower in patients with <i>H. pylori</i> infection, compared with that of the ones without the infection (<i>P</i> = 0.001); but there was no significant difference between the cases with and without <i>H. pylori</i> infection regarding the level of LDL and HDL. The mean fasting blood sugar (FBS) in the cases with <i>H. pylori</i> infection was significantly higher than that of the ones without the infection (<i>P</i> = 0.04).</p><p><strong>Conclusion: </strong>According to the results of the current study, the levels of LDL and FBS were high among the male cases with <i>H. pylori</i> infection. However, in females with <i>H. pylori</i> infection the level of TG was low; hence, it seems that the atherogenicity of <i>H. pylori</i> affected the level of blood sugar more.</p>","PeriodicalId":16274,"journal":{"name":"Journal of Lipids","volume":"2018 ","pages":"6734809"},"PeriodicalIF":5.3,"publicationDate":"2018-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2018/6734809","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36285520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Utilization of Solid Waste as a Substrate for Production of Oil from Oleaginous Microorganisms.","authors":"Fortunate Laker,&nbsp;Arnold Agaba,&nbsp;Andrew Akatukunda,&nbsp;Robert Gazet,&nbsp;Joshua Barasa,&nbsp;Sarah Nanyonga,&nbsp;Deborah Wendiro,&nbsp;Alex Paul Wacoo","doi":"10.1155/2018/1578720","DOIUrl":"https://doi.org/10.1155/2018/1578720","url":null,"abstract":"<p><p>The overwhelming demand of oil and fats to meet the ever increasing needs for biofuel, cosmetics production, and other industrial purposes has enhanced a number of innovations in this industry. One such innovation is the use of microorganisms as alternative sources of oil and fats. Organic solid waste that is causing a big challenge of disposal worldwide is biodegradable and can be utilized as substrate for alternative oil production. The study evaluated the potential of isolated yeast-like colonies to grow and accumulate oil by using organic solid waste as substrate. Of the 25 yeast-like colonies isolated from the soil samples collected from three different suburbs in Kampala district, Uganda, 20 were screened positive for accumulation of lipid but only 2 were oleaginous. The NHC isolate with the best oil accumulation potential of 48.8% was used in the central composite design (CCD) experiments. The CCD experimental results revealed a maximum oil yield of 61.5% from 1.25 g/L cell biomass at 10 g/L of solid waste and temperature of 25°C. The study revealed that organic solid waste could be used as a substrate for microbial oil production.</p>","PeriodicalId":16274,"journal":{"name":"Journal of Lipids","volume":"2018 ","pages":"1578720"},"PeriodicalIF":5.3,"publicationDate":"2018-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2018/1578720","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36189344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Locally Manufactured Niger Seed Oil on Lipid Profile Compared to Imported Palm and Sunflower Oils on Rat Models.","authors":"Zewdie Mekonnen,&nbsp;Abrha Gebreselema,&nbsp;Yohannes Abere","doi":"10.1155/2018/7846350","DOIUrl":"https://doi.org/10.1155/2018/7846350","url":null,"abstract":"<p><strong>Background: </strong>Different types of dietary lipids have been shown to affect lipid metabolism and lipid profile differently.</p><p><strong>Objective: </strong>This study aims to assess the effect of local niger seed oil on serum lipid profile compared to palm oil and sunflower oil in rats.</p><p><strong>Methods: </strong>The effect of the 15% plant oils on serum lipid profile, body weight gain percentage, and feed efficiency ratio was assessed after 8 weeks of experimental period.</p><p><strong>Results and conclusion: </strong>The 15% niger seed oil showed decrease and increase in the level of lipid profile as compared to rats fed with 15% palm oil and sunflower oil (except Triacylglycerol), respectively. The 15% niger seed oil showed significant decrease and increase in body weight gain percentage as compared to the 15% palm oil and 15% sunflower oil, respectively. The feed efficiency ratio was significantly higher and lower in the 15% niger seed oil compared to rats fed with 15% sunflower oil and control group and the palm oil fed rats, respectively. The current study concluded that consumption of locally manufactured niger seed oil decreased the blood lipid profiles, body weight gain percentage, and feed efficiency ratio as compared to palm oil. Utilization of oils containing more unsaturated fatty acids like niger seed oil is recommended to reduce the risk of developing cardiovascular disease.</p>","PeriodicalId":16274,"journal":{"name":"Journal of Lipids","volume":"2018 ","pages":"7846350"},"PeriodicalIF":5.3,"publicationDate":"2018-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2018/7846350","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36181908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Safety and Efficacy of Extremely Low LDL-Cholesterol Levels and Its Prospects in Hyperlipidemia Management.","authors":"Dhrubajyoti Bandyopadhyay, Arshna Qureshi, Sudeshna Ghosh, Kumar Ashish, Lyndsey R Heise, Adrija Hajra, Raktim K Ghosh","doi":"10.1155/2018/8598054","DOIUrl":"10.1155/2018/8598054","url":null,"abstract":"<p><p>The risk of cardiovascular disease has been reported to have a linear relationship with LDL levels. Additionally, the currently recommended LDL target goal of 70 mg/dl does not diminish the CV risk entirely leaving behind some residual risk. Previous attempts to maximally lower the LDL levels with statin monotherapy have met dejection due to the increased side effects associated with the treatment. Nevertheless, with the new advancements in clinical medicine, it has now become possible to bring down the LDL levels to as low as 15 mg/dl using PCSK9 monoclonal antibodies alone or in combination with statins. The development of inclisiran, siRNA silencer targeting PCSK9 gene, is a one step forward in these endeavors. Moreover, various studies aiming to lower the CV risk and mortality by lowering LDL levels have demonstrated encouraging results. The current challenge is to explore this arena to redefine the target LDL levels, if required, to avoid any suboptimal treatment. After thorough literature search in the PubMed, Embase, Scopus, and Google Scholar, we present this article to provide a brief overview of the safety and efficacy of lowering LDL below the current goal.</p>","PeriodicalId":16274,"journal":{"name":"Journal of Lipids","volume":"2018 ","pages":"8598054"},"PeriodicalIF":5.3,"publicationDate":"2018-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2018/8598054","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36178366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}