Journal of Innovative Optical Health Sciences最新文献

{"title":"Self-Confocal NIR-II fluorescence microscopy for multifunctional <i>in vivo</i> imaging","authors":"Jing Zhou, Tianxiang Wu, Runze Chen, Liang Zhu, Hequn Zhang, Yifei Li, Liying Chen, Jun Qian","doi":"10.1142/s1793545823500256","DOIUrl":"https://doi.org/10.1142/s1793545823500256","url":null,"abstract":"Fluorescence imaging in the second near-infrared window (NIR-II, 900–1880[Formula: see text]nm) with less scattering background in biological tissues has been combined with the confocal microscopic system for achieving deep in vivo imaging with high spatial resolution. However, the traditional NIR-II fluorescence confocal microscope with separate excitation focus and detection pinhole makes it possess low confocal efficiency, as well as difficultly to adjust. Two types of upgraded NIR-II fluorescence confocal microscopes, sharing the same pinhole by excitation and emission focus, leading to higher confocal efficiency, are built in this work. One type is fiber-pinhole-based confocal microscope applicable to CW laser excitation. It is constructed for fluorescence intensity imaging with large depth, high stabilization and low cost, which could replace multiphoton fluorescence microscopy in some applications (e.g., cerebrovascular and hepatocellular imaging). The other type is air-pinhole-based confocal microscope applicable to femtosecond (fs) laser excitation. It can be employed not only for NIR-II fluorescence intensity imaging, but also for multi-channel fluorescence lifetime imaging to recognize different structures with similar fluorescence spectrum. Moreover, it can be facilely combined with multiphoton fluorescence microscopy. A single fs pulsed laser is utilized to achieve up-conversion (visible multiphoton fluorescence) and down-conversion (NIR-II one-photon fluorescence) excitation simultaneously, extending imaging spectral channels, and thus facilitates multi-structure and multi-functional observation.","PeriodicalId":16248,"journal":{"name":"Journal of Innovative Optical Health Sciences","volume":"47 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135343822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of central and obstructive respiratory events on cerebral oxygenation in adults with sleep disordered breathing","authors":"Ramin Khatami, Dominik Gnaiger, Gordana Hügli, Ming Qi, Zhongxing Zhang","doi":"10.1142/s1793545823400047","DOIUrl":"https://doi.org/10.1142/s1793545823400047","url":null,"abstract":"Obstructive sleep apnea (OSA) and central sleep apnea (CSA) are two main types of sleep disordered breathing (SDB). While the changes in cerebral hemodynamics triggered by OSA events have been well studied using near-infrared spectroscopy (NIRS), they are essentially unknown in CSA in adults. Therefore, in this study, we compared the changes in cerebral oxygenation between OSA and CSA events in adult patients using NIRS. Cerebral tissue oxygen saturation (StO 2 ) in 13 severe SDB patients who had both CSA and OSA events was measured using frequency-domain NIRS. The changes in cerebral StO 2 desaturation and blood volume (BV) in the first hour of natural sleep were compared between different types of respiratory events (i.e., 277 sleep hypopneas, 161 OSAs and 113 CSAs) with linear mixed-effect models controlling for confounders. All respiratory events occurred during non-rapid eye movement (NREM) sleep. We found that apnea events induced greater cerebral desaturations and BV fluctuations compared to hypopneas, but there was no difference between OSA and CSA. These results suggest that cerebral autoregulation in our patients are still capable to counteract the pathomechanisms of apneas, in particularly the negative intrathoracic pressure (ITP) caused by OSA events. Otherwise larger BV fluctuations in OSA compared to CSA should be observed due to the negative ITP that reduces cardiac stroke volume and leads to lower systematic blood supply. Our study suggests that OSA and CSA may have similar impact on cerebral oxygenation during NREM sleep in adult patients with SDB.","PeriodicalId":16248,"journal":{"name":"Journal of Innovative Optical Health Sciences","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136144202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nonlinear optical imaging by detection with optical parametric amplification (invited paper).","authors":"Yi Sun, Haohua Tu, Stephen A Boppart","doi":"10.1142/s1793545822450018","DOIUrl":"10.1142/s1793545822450018","url":null,"abstract":"<p><p>Nonlinear optical imaging is a versatile tool that has been proven to be exceptionally useful in various research fields. However, due to the use of photomultiplier tubes (PMTs), the wide application of nonlinear optical imaging is limited by the incapability of imaging under ambient light. In this paper, we propose and demonstrate a new optical imaging detection method based on optical parametric amplification (OPA). As a nonlinear optical process, OPA intrinsically rejects ambient light photons by coherence gating. Periodical poled lithium niobate (PPLN) crystals are used in this study as the media for OPA. Compared to bulk nonlinear optical crystals, PPLN crystals support the generation of OPA signal with lower pump power. Therefore, this characteristic of PPLN crystals is particularly beneficial when using high-repetition-rate lasers, which facilitate high-speed optical signal detection, such as in spectroscopy and imaging. A PPLN-based OPA system was built to amplify the emitted imaging signal from second harmonic generation (SHG) and coherent anti-Stokes Raman scattering (CARS) microscopy imaging, and the amplified optical signal was strong enough to be detected by a biased photodiode under ordinary room light conditions. With OPA detection, ambient-light-on SHG and CARS imaging becomes possible, and achieves a similar result as PMT detection under strictly dark environments. These results demonstrate that OPA can be used as a substitute for PMTs in nonlinear optical imaging to adapt it to various applications with complex lighting conditions.</p>","PeriodicalId":16248,"journal":{"name":"Journal of Innovative Optical Health Sciences","volume":"16 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10426456/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10394565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Broadband diffuse optical spectroscopy of two-layered scattering media containing oxyhemoglobin, deoxyhemoglobin, water, and lipids.","authors":"Giles Blaney, Martina Bottoni, Angelo Sassaroli, Cristianne Fernandez, Sergio Fantini","doi":"10.1142/s1793545822500201","DOIUrl":"10.1142/s1793545822500201","url":null,"abstract":"<p><p>We investigated the relationship between chromophore concentrations in two-layered scattering media and the apparent chromophore concentrations measured with broadband optical spectroscopy in conjunction with commonly used homogeneous medium inverse models. We used diffusion theory togenerate optical data from a two-layered distribution of relevant tissue absorbers, namely, oxyhemoglobin, deoxyhemoglobin, water, and lipids, with a top-layer thickness in the range 1-15 mm. The generated data consisted of broadband continuous-wave (CW) diffuse reflectance in the wavelength range 650-1024 nm, and frequency-domain (FD) diffuse reflectance at 690 and 830 nm; two source-detector distances of 25 and 35 mm were used to simulate a dual-slope technique. The data were inverted using diffusion theory for a semi-infinite homogeneous medium to generate reduced scattering coefficients at 690 and 830 nm (from FD data) and effective absorption spectra in the range 650-1024 nm (from CW data). The absorption spectra were then converted into effective total concentration and oxygen saturation of hemoglobin, as well as water and lipid concentrations. For absolute values, it was found that the effective hemoglobin parameters are typically representative of the bottom layer, whereas water and lipid represent some average of the respective concentrations in the two layers. For concentration changes, lipid showed a significant cross-talk with other absorber concentrations, thus indicating that lipid dynamics obtained in these conditions may not be reliable. These systematic simulations of broadband spectroscopy of two-layered media provide guidance on how to interpret effective optical properties measured with similar instrumental setups under the assumption of medium homogeneity.</p>","PeriodicalId":16248,"journal":{"name":"Journal of Innovative Optical Health Sciences","volume":"15 3","pages":""},"PeriodicalIF":2.3,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9203000/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40024809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of cerebral oxygenation response to hemodialysis using near-infrared spectroscopy (NIRS): Challenges and solutions.","authors":"Ardy Wong,&nbsp;Lucy Robinson,&nbsp;Seena Soroush,&nbsp;Aditi Suresh,&nbsp;Dia Yang,&nbsp;Kelechi Madu,&nbsp;Meera N Harhay,&nbsp;Kambiz Pourrezaei","doi":"10.1142/s1793545821500164","DOIUrl":"https://doi.org/10.1142/s1793545821500164","url":null,"abstract":"<p><p>To date, the clinical use of functional near-infrared spectroscopy (NIRS) to detect cerebral ischemia has been largely limited to surgical settings, where motion artifacts are minimal. In this study, we present novel techniques to address the challenges of using NIRS to monitor ambulatory patients with kidney disease during approximately eight hours of hemodialysis (HD) treatment. People with end-stage kidney disease who require HD are at higher risk for cognitive impairment and dementia than age-matched controls. Recent studies have suggested that HD-related declines in cerebral blood flow might explain some of the adverse outcomes of HD treatment. However, there are currently no established paradigms for monitoring cerebral perfusion in real-time during HD treatment. In this study, we used NIRS to assess cerebral hemodynamic responses among 95 prevalent HD patients during two consecutive HD treatments. We observed substantial signal attenuation in our predominantly Black patient cohort that required probe modifications. We also observed consistent motion artifacts that we addressed by developing a novel NIRS methodology, called the HD cerebral oxygen demand algorithm (HD-CODA), to identify episodes when cerebral oxygen demand might be outpacing supply during HD treatment. We then examined the association between a summary measure of time spent in cerebral deoxygenation, derived using the HD-CODA, and hemodynamic and treatment-related variables. We found that this summary measure was associated with intradialytic mean arterial pressure, heart rate, and volume removal. Future studies should use the HD-CODA to implement studies of real-time NIRS monitoring for incident dialysis patients, over longer time frames, and in other dialysis modalities.</p>","PeriodicalId":16248,"journal":{"name":"Journal of Innovative Optical Health Sciences","volume":"14 6","pages":""},"PeriodicalIF":2.5,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8846418/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39806429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photoacoustic detection of circulating melanoma cells in late stage patients.","authors":"John A Viator,&nbsp;Marc Hazur,&nbsp;Andrea Sajewski,&nbsp;Ahmad Tarhini,&nbsp;Martin E Sanders,&nbsp;Robert H Edgar","doi":"10.1142/s1793545820500236","DOIUrl":"https://doi.org/10.1142/s1793545820500236","url":null,"abstract":"<p><p>Melanoma is the deadliest skin cancer and is responsible for over 7000 deaths in the US annually. The spread of cancer, or metastasis, is responsible for these deaths, as secondary tumors interrupt normal organ function. Circulating tumor cells, or those cells that spread throughout the body from the primary tumor, are thought to be responsible for metastasis. We developed an optical method, photoacoustic flow cytometry, in order to detect and enumerate circulating melanoma cells (CMCs) from blood samples of patients. We tested the blood of Stage IV melanoma patients to show the ability of the photoacoustic flow cytometer to detect these rare cells in blood. We then tested the system on archived blood samples from Stage III melanoma patients with known outcomes to determine if detection of CMCs can predict future metastasis. We detected between 0 and 66 CMCs in Stage IV patients. For the Stage III study, we found that of those samples with CMCs, 2 remained disease free and 5 developed metastasis. Of those without CMCs, 6 remained disease free and 1 developed metastasis. We believe that photoacoustic detection of CMCs provides valuable information for the prediction of metastasis and we postulate a system for more accurate prognosis.</p>","PeriodicalId":16248,"journal":{"name":"Journal of Innovative Optical Health Sciences","volume":"13 6","pages":""},"PeriodicalIF":2.5,"publicationDate":"2020-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8218985/pdf/nihms-1606057.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39099544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Video-rate multimodal multiphoton imaging and three-dimensional characterization of cellular dynamics in wounded skin.","authors":"Joanne Li, Madison N Wilson, Andrew J Bower, Marina Marjanovic, Eric J Chaney, Ronit Barkalifa, Stephen A Boppart","doi":"10.1142/s1793545820500078","DOIUrl":"10.1142/s1793545820500078","url":null,"abstract":"<p><p>To date, numerous studies have been performed to elucidate the complex cellular dynamics in skin diseases, but few have attempted to characterize these cellular events under conditions similar to the native environment. To address this challenge, a three-dimensional (3D) multimodal analysis platform was developed for characterizing <i>in vivo</i> cellular dynamics in skin, which was then utilized to process <i>in vivo</i> wound healing data to demonstrate its applicability. Special attention is focused on <i>in vivo</i> biological parameters that are difficult to study with <i>ex vivo</i> analysis, including 3D cell tracking and techniques to connect biological information obtained from different imaging modalities. These results here open new possibilities for evaluating 3D cellular dynamics <i>in vivo</i>, and can potentially provide new tools for characterizing the skin microenvironment and pathologies in the future.</p>","PeriodicalId":16248,"journal":{"name":"Journal of Innovative Optical Health Sciences","volume":"13 2","pages":""},"PeriodicalIF":2.5,"publicationDate":"2020-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1142/s1793545820500078","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25367954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multimodal intravascular imaging technology for characterization of atherosclerosis.","authors":"Yan Li, Jason Chen, Zhongping Chen","doi":"10.1142/s1793545820300013","DOIUrl":"10.1142/s1793545820300013","url":null,"abstract":"<p><p>Early detection of vulnerable plaques is the critical step in the prevention of acute coronary events. Morphology, composition, and mechanical property of a coronary artery have been demonstrated to be the key characteristics for the identification of vulnerable plaques. Several intravascular multimodal imaging technologies providing co-registered simultaneous images have been developed and applied in clinical studies to improve the characterization of atherosclerosis. In this paper, the authors review the present system and probe designs of representative intravascular multimodal techniques. In addition, the scientific innovations, potential limitations, and future directions of these technologies are also discussed.</p>","PeriodicalId":16248,"journal":{"name":"Journal of Innovative Optical Health Sciences","volume":"13 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7164814/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37851334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Near-infrared spectroscopy muscle oximetry of patients with postural orthostatic tachycardia syndrome.","authors":"Parvathi Kadamati, Jeffrey J Sugar, Brendan J Quirk, Shima Mehrvar, Gisela G Chelimsky, Harry T Whelan, Thomas C Chelimsky, Mahsa Ranji","doi":"10.1142/S1793545818500268","DOIUrl":"10.1142/S1793545818500268","url":null,"abstract":"<p><p>Postural orthostatic tachycardia syndrome (POTS) is a disabling condition characterized by orthostatic intolerance with tachycardia in the absence of drop-in blood pressure. A custom-built near-infrared spectroscopy device (NIRS) is applied to monitor the muscle oxygenation, noninvasively in patients undergoing incremental head-up tilt table (HUT). Subjects (6 POTS patients and 6 healthy controls) underwent 30 mins of 70°on a HUT. The results showed a significant difference in deoxyhemoglobin (Hb), change-in-oxygenation (ΔOxy) and blood volume (ΔBV) between patients and healthy controls. However, oxyhemoglobin (HbO₂) showed a significantly faster rate of change in the healthy controls during the first 10 mins of the tilt and during the recovery. This NIRS muscle oximetry tool provides quantitative measurements of blood oxygenation monitoring in diseases such as POTS.</p>","PeriodicalId":16248,"journal":{"name":"Journal of Innovative Optical Health Sciences","volume":"11 6","pages":""},"PeriodicalIF":2.5,"publicationDate":"2018-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6124683/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36475813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative optical measurement of mitochondrial superoxide dynamics in pulmonary artery endothelial cells.","authors":"Zahra Ghanian,&nbsp;Girija Ganesh Konduri,&nbsp;Said Halim Audi,&nbsp;Amadou K S Camara,&nbsp;Mahsa Ranji","doi":"10.1142/S1793545817500183","DOIUrl":"https://doi.org/10.1142/S1793545817500183","url":null,"abstract":"<p><p>Reactive oxygen species (ROS) play a vital role in cell signaling and redox regulation, but when present in excess, lead to numerous pathologies. Detailed quantitative characterization of mitochondrial superoxide anion ( <math><mrow><msubsup><mi>O</mi><mn>2</mn><mrow><mo>•</mo><mo>-</mo></mrow></msubsup></mrow></math> ) production in fetal pulmonary artery endothelia cells (PAECs) has never been reported. The aim of this study is to assess mitochondrial <math><mrow><msubsup><mi>O</mi><mn>2</mn><mrow><mo>•</mo><mo>-</mo></mrow></msubsup></mrow></math> production in cultured PAECs over time using a novel quantitative optical approach. The rate, the sources, and the dynamics of <math><mrow><msubsup><mi>O</mi><mn>2</mn><mrow><mo>•</mo><mo>-</mo></mrow></msubsup></mrow></math> production were assessed using targeted metabolic modulators of the mitochondrial electron transport chain (ETC) complexes, specifically an uncoupler and inhibitors of the various ETC complexes, and inhibitors of extra-mitochondrial sources of <math><mrow><msubsup><mi>O</mi><mn>2</mn><mrow><mo>•</mo><mo>-</mo></mrow></msubsup></mrow></math> . After stabilization, the cells were loaded with nanomolar mitochondrial-targeted hydroethidine (Mito-HE, MitoSOX) online during the experiment without washout of the residual dye. Time-lapse fluorescence microscopy was used to monitor the dynamic changes in <math><mrow><msubsup><mi>O</mi><mn>2</mn><mrow><mo>•</mo><mo>-</mo></mrow></msubsup></mrow></math> fluorescence intensity over time in PAECs. The transient behaviors of the fluorescence time course showed exponential increases in the rate of <math><mrow><msubsup><mi>O</mi><mn>2</mn><mrow><mo>•</mo><mo>-</mo></mrow></msubsup></mrow></math> production in the presence of the ETC uncoupler or inhibitors. The most dramatic and the fastest increase in <math><mrow><msubsup><mi>O</mi><mn>2</mn><mrow><mo>•</mo><mo>-</mo></mrow></msubsup></mrow></math> production was observed when the cells were treated with the uncoupling agent, PCP. We also showed that only the complex IV inhibitor, KCN, attenuated the marked surge in <math><mrow><msubsup><mi>O</mi><mn>2</mn><mrow><mo>•</mo><mo>-</mo></mrow></msubsup></mrow></math> production induced by PCP. The results showed that mitochondrial respiratory complexes I, III and IV are sources of <math><mrow><msubsup><mi>O</mi><mn>2</mn><mrow><mo>•</mo><mo>-</mo></mrow></msubsup></mrow></math> production in PAECs, and a new observation that ROS production during uncoupling of mitochondrial respiration is mediated in part via complex IV. This novel method can be applied in other studies that examine ROS production under stress condition and during ROS-mediated injuries <i>in vitro</i>.</p>","PeriodicalId":16248,"journal":{"name":"Journal of Innovative Optical Health Sciences","volume":"11 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1142/S1793545817500183","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36411159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}