Journal of King Saud University - Science最新文献

{"title":"Aberrant expression of miR-143/miR-223/miR4478 and miR145 as prognostic factor for colorectal cancer patients","authors":"Abdullah M Alhadheq ,&nbsp;Rana Alhadheq ,&nbsp;Saad Alkahtani","doi":"10.1016/j.jksus.2024.103494","DOIUrl":"10.1016/j.jksus.2024.103494","url":null,"abstract":"<div><div>Colorectal cancer (CRC) is a type of malignancy that develops in the colon or rectal region. It develops due to abnormal growth and proliferation of cells in the lining of the colon, forming a tumor. This study included tissue samples (56 malignant and 56 matched normal samples). miRNAs were determined and retrieved from miRBase database and moderated by p53 gene. Expression levels of miRNA were varied. Only miR-223 revealed greater expression than normal matched tissue. mMiR-143, miR-4478, and miR-145 exhibited lower expression than matched normal tissue. Only miR-143 and miR-145 showed considerable variations in the expression among groups while miR-4478 did not demonstrate statistically substantial difference. In conclusion, this study highlights that upregulation of P53 and miR-223 and downregulation of miR-145 and miR-143 were associated with cancer advancement and unfavorable prognosis in Saudi CRC patients, indicating P53, miR-223, miR-145 and miR-143 to be novel and valuable signatures for predicting the outcomes for patients with CRC.</div></div>","PeriodicalId":16205,"journal":{"name":"Journal of King Saud University - Science","volume":"36 11","pages":"Article 103494"},"PeriodicalIF":3.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142534699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparative study of chemical composition, the level of fatty acids, amino acids and mineral elements between two types of catfish (Bagrus and Muraena) found in the upper Tigris River","authors":"Tariq Kh. Albashr ,&nbsp;Ahmed Y. Hamadamin ,&nbsp;Karzan F. Namiq ,&nbsp;Miran H. Salih ,&nbsp;Zaid K. Khidhir ,&nbsp;M.A. Suhaib","doi":"10.1016/j.jksus.2024.103535","DOIUrl":"10.1016/j.jksus.2024.103535","url":null,"abstract":"<div><div>Fish meat is considered the second most interesting to consume in Iraq hence, consumers always consider the sensory and nutritional profile of the fish meat. The study aimed to investigate the chemical composition and level of fatty acids and amino acids in <em>Bagrus</em> and <em>Muraena</em> fish fillets. These fish for this study were obtained from the upper Tigris river in the Tikrit region of Iraq, as the demand for these types of fish increased in recent years. In this work, High Performance Liquid Chromatography −UV (HPLC-UV) was used for quantification of amino acids, and atomic absorption was used for quantifying the elements. The extraction was performed by liquid –liquid extraction and purified by dispersive phase purification. Results showed that the moisture percentages in <em>Bagrus</em> meat were 71.36 %, while <em>Muraena</em> meat recorded as 71.10 %. Moreover, the protein percentages for both fish types were found as 21.36 and 21.43  % respectively. Lipid percentages in fillets of <em>Bagrus</em> and <em>Muraena</em> were 3.59 and 4.62 %, respectively. The proportion of ash was 3.01 % in <em>Bagrus</em> meat, while it was 2.55 % in <em>Muraena</em> meat. The results also presented that the <em>Muraena</em> has a significantly higher saturated fatty acid level (26.06 %), whereas in <em>Bagrus</em> meat was much less (16.6 %). The highest percentage of amino acids in <em>Bagrus</em> meat recorded (92.66 %), while it was 61.94 % in <em>Muraena</em> meat. The <em>Bagrus</em> meat contained essential amino acids with a higher percentage (54.04 %), compared to <em>Muraena</em> meat (28.55 %), non-essential Amino acids percentage also reached 47.56 % in <em>Bagrus</em> fish fillets. The amino acids like alanine had recorded the highest percentage among non-essential amino acids, as its level resulted in 38.62 %, while the level (33.39 %) was lower in <em>Muraena</em> meat.</div></div>","PeriodicalId":16205,"journal":{"name":"Journal of King Saud University - Science","volume":"36 11","pages":"Article 103535"},"PeriodicalIF":3.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142658832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Facile synthesis of cerium doped Co-Mn-FeO nano ferrites as highly sensitive and fast response humidity sensors at room temperature","authors":"S.A. Al-Ghamdi","doi":"10.1016/j.jksus.2024.103537","DOIUrl":"10.1016/j.jksus.2024.103537","url":null,"abstract":"<div><div>Herein, this work presents the facile synthesis of Cerium (Ce³⁺) doped Cobalt-manganese-iron oxide ferrite as an effective humidity sensor operated at ambient temperature. This work reports, one step solution combustion technique to synthesize Co_0.5Mn_0.5Fe_2−xCe_xO₄ (x = 0.0, 0.050 and 0.1) ferrites. The structural and morphological features of the synthesized Co_0.5Mn_0.5Fe_2−xCe_xO₄ ferrites NPs were investigated through scanning electron microscope (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and ultraviolet visible (UV–visible) spectroscopy. The XRD results indicates the creation of single phase in pure Co_0.5Mn_0.5Fe_2−xCe_xO₄ ferrite that remains unchanged by the mechanism of Ce³⁺inclusion. The humidity sensing mechanism was systematically investigated using adsorption–desorption isotherms. Highest humidity sensing response was achieved for Co_0.5Mn_0.5Fe_2−xCe_xO₄ (x = 0.1) ferrite of the order of 98 % in comparison to the pure Co_0.5Mn_0.5Fe_2−xCe_xO₄ (x = 0) which is about 41 %. The sensors response and recovery times for the Co_0.5Mn_0.5Fe_2−xCe_xO₄ (x = 0.1) was recorded to be 36 s and 63 s respectively and the sensors long term stability was examined for a duration of 90 days. The results of the present investigation offer new dimensions to fabricate high performance, room temperature operable humidity sensors for next generation sensors applications.</div></div>","PeriodicalId":16205,"journal":{"name":"Journal of King Saud University - Science","volume":"36 11","pages":"Article 103537"},"PeriodicalIF":3.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142658915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An inertial conjugate gradient projection method for large-scale nonlinear equations and its application in the image restoration problems","authors":"Gonglin Yuan ,&nbsp;Chunzhao Liang ,&nbsp;Yong Li","doi":"10.1016/j.jksus.2024.103547","DOIUrl":"10.1016/j.jksus.2024.103547","url":null,"abstract":"<div><div>Based on the acceleration effect of the inertial extrapolation technique on the convergence of iterative sequences, the number of algorithms incorporating this technique has gradually increased in recent years. Currently, there is a relative paucity of studies focusing on the Polak-Ribière-Polyak (PRP) conjugate gradient algorithm that integrate the inertial extrapolation technique. In this article, we introduce an inertial three-term PRP conjugate gradient projection method by incorporating an inertial extrapolation step into the three-term PRP algorithm, where the search direction exhibits sufficient descent and trust region characteristics. The search rule employs a derivative-free technique. Under suitable hypotheses, the proposed algorithm demonstrates global convergence. Numerical results indicate the superiority and competitiveness of this innovative method. Furthermore, its effectiveness in addressing image restoration problems underscores the practicality of this algorithm.</div></div>","PeriodicalId":16205,"journal":{"name":"Journal of King Saud University - Science","volume":"36 11","pages":"Article 103547"},"PeriodicalIF":3.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143152104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytotoxic effect of polystyrene nanoplastics in human umbilical vein endothelial cells (HUVECs) and normal rat kidney cells (NRK52E)","authors":"Maqusood Ahamed,&nbsp;Mohd Javed Akhtar","doi":"10.1016/j.jksus.2024.103505","DOIUrl":"10.1016/j.jksus.2024.103505","url":null,"abstract":"<div><div>Plastics play a crucial role in nearly each aspect of societal production and everyday life, therefore making them one of the most widespread pollutants on a global scale. Because synthetic plastics are not entirely biodegradable, they often remain in the environment and fragment into micro- and nanoplastic particles. The detrimental impacts of nanoplastics on the environment and human health have received substantial worldwide interest in recent years. However, the effects of nanoplastics on human health have not yet been fully explored. Our study aimed to investigate the cytotoxic effects of polystyrene nanoplastics on two distinct mammalian cell lines: normal rat kidney cells (NRK52E) and human umbilical vein endothelial cells (HUVECs). Results showed that polystyrene nanoplastics generate cytotoxicity in both NRK52E and HUVECs in a concentration- and time-dependent manner. Additionally, polystyrene nanoplastics induced pro-oxidant levels (e.g., reactive oxygen species and hydrogen peroxide) and reduced antioxidants (glutathione content and glutathione peroxidase enzyme activity) in both types of cells. We also found that polystyrene nanoplastics cause apoptosis in both NRK52E and HUVECs, as shown by the activation of the caspase-3 enzyme and the loss of mitochondrial membrane potential. Interestingly, it was noticed that the vulnerability of HUVECs cells against polystyrene nanoplastics was a little higher than that of NRK52E cells. Also, the cell toxicity caused by polystyrene nanoplastics in NRK52E and HUVECs was effectively alleviated by co-exposure to a reactive oxygen species inhibitor, N-acetyl-cysteine. This suggests that oxidative stress could be one of the possible pathways by which polystyrene nanoplastics cause cell toxicity. The present work warrants future study to explore the toxicity mechanisms of polystyrene nanoplastics in appropriate <em>in vivo</em> models.</div></div>","PeriodicalId":16205,"journal":{"name":"Journal of King Saud University - Science","volume":"36 11","pages":"Article 103505"},"PeriodicalIF":3.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142561168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Copper and chromium binding by Pseudomonas aeruginosa strain PA01 for implications of heavy metal detoxification and soil remediation: A computational approach","authors":"Shanmuga Priya Ramasamy ,&nbsp;Priya Sundararajan ,&nbsp;Muthukrishnan Pallikondaperumal ,&nbsp;Ponmurugan Karuppiah ,&nbsp;Saminathan Kayarohanam ,&nbsp;Natarajan Arumugam ,&nbsp;Ling Shing Wong ,&nbsp;Sinouvassane Djearamane","doi":"10.1016/j.jksus.2024.103552","DOIUrl":"10.1016/j.jksus.2024.103552","url":null,"abstract":"<div><div>Heavy metal pollution poses significant environmental and health risks due to the toxic effects of metals like copper and chromium at elevated concentrations. Despite their essential roles in trace amounts, these metals can be highly toxic. Bacteria such as<!--> <em>Pseudomonas aeruginosa</em> <!-->are promising candidates for bioremediation due to their robustness and adaptability.<!--> <!-->The objective of this study was to analyze and identify potential copper and chromium binding genes involved in metal detoxification in<!--> <em>Pseudomonas aeruginosa</em> <!-->PA01. The heavy metal binding protein identified as ferredoxin using MALDI-TOF/PMF-MS analysis was further characterized. The structure of the ferredoxin protein was elucidated using the SWISS-MODEL tool. Metal-binding domains were validated through a pattern search against UniProtKB/Swiss-Prot and UniProtKB/TrEMBL databases using the ScanProsite tool. Comparative sequence alignments were conducted between the copper-binding NosD gene of<!--> <em>P. aeruginosa</em>, the ferredoxin gene of<!--> <em>P. aeruginosa</em> <!-->PA01, and the chromium-binding iron hydrogenase 1 gene of<!--> <em>Clostridium chromiireducens</em>. The SWISS-MODEL analysis revealed alpha helices and beta sheets with key metal-coordinating amino acids (cysteine, glutamic acid, aspartic acid, histidine, and methionine). The ScanProsite tool confirmed the presence of a 4Fe-4S ferredoxin-type iron-sulphur binding domain essential for coordinating chromium and copper ions. Sequence alignments showed a 64.29 % similarity between the NosD gene and ferredoxin gene, and a 67 % identity between the iron hydrogenase 1 gene and ferredoxin gene, with correlations in amino acid residues involved in metal binding. These findings suggest that the ferredoxin gene could effectively bind heavy metal ions, offering potential applications in bioremediation of metal-polluted soils using<!--> <em>Pseudomonas</em> <!-->species. This study contributes to sustainable agricultural productivity by facilitating the targeted remediation of heavy metal-contaminated soils through biological means.</div></div>","PeriodicalId":16205,"journal":{"name":"Journal of King Saud University - Science","volume":"36 11","pages":"Article 103552"},"PeriodicalIF":3.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142748182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Understanding the impact of deep structures on the hydrological setting of the Eastern Bahira Basin in Morocco using combined geophysical analysis of gravity, seismic, and electrical resistivity data","authors":"Anas Charbaoui ,&nbsp;Mourad Guernouche ,&nbsp;Azzouz Kchikach ,&nbsp;Mohammed Jaffal ,&nbsp;Abdessamad Hadri ,&nbsp;Mihai Octavian Cimpoiasu ,&nbsp;Samuel Zappalá ,&nbsp;Mounir Amar ,&nbsp;Oussama Khadiri Yazami","doi":"10.1016/j.jksus.2024.103491","DOIUrl":"10.1016/j.jksus.2024.103491","url":null,"abstract":"<div><div>In arid regions like the Eastern Bahira Basin, communities mainly rely on groundwater for drinking and irrigation. However, efficiently managing these vital resources requires a deep understanding of the underlying aquifers’ structure and identifying the most suitable areas for exploitation. This presents a significant challenge for the success of water supply and irrigation programs in the Eastern Bahira Basin. This study is based on an integrative approach, combining Electrical Resistivity Tomography data, with the compilation and reinterpretation of pre-existing seismic, gravimetric and vertical electrical sounding data. This approach is based on compiling old gravimetric data and applying advanced processing techniques to determine the horizontal gradient maxima, which helps highlight the major structural alignments in the basin. Furthermore, the approach utilizes seismic data in order to enhance understanding of the deep structure of the basin, reinterpreting it in light of recent drilling data. The interpretation of the gravimetric and seismic data has also been validated by the results of vertical electrical soundings and electrical tomography that we recently acquired in the Eastern Bahira basin. The outcomes of this research provide new insights into the deep structure of the Eastern Bahira Basin and suggest the most promising hydrogeological prospects, thereby contributing to the success of the ongoing drinking water supply and irrigation program in the Eastern Bahira Basin.</div></div>","PeriodicalId":16205,"journal":{"name":"Journal of King Saud University - Science","volume":"36 11","pages":"Article 103491"},"PeriodicalIF":3.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142534693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reno-protective potential of poncirin against polyethylene microplastics instigated kidney damage in rats via regulating Nrf-2/Keap-1 pathway","authors":"Muhammad Faisal Hayat ,&nbsp;Maryam Javed ,&nbsp;Rahat Andleeb ,&nbsp;Asma Ashraf ,&nbsp;Huma Naz ,&nbsp;Mohammad Z. Ahmed ,&nbsp;Ayesha Ishtiaq","doi":"10.1016/j.jksus.2024.103486","DOIUrl":"10.1016/j.jksus.2024.103486","url":null,"abstract":"<div><div>Polyethylene microplastics (PEMPs) are noxious environmental pollutants that are documented to cause organ damage including the kidneys. Poncirin (PON) is a naturally occurring flavonoid which demonstrates a wide range of pharmacological properties. This experiment was conducted to evaluate the palliative potential of PON against PEMPs induced renal toxicity by examining a range of biochemical and physiological parameters. Twenty-four male albino rats were randomly apportioned into four distinct groups including the control, PEMPs (1.5 mgkg⁻¹), PEMPs (1.5 mgkg⁻¹) + PON (20 mgkg⁻¹) and only PON (20 mgkg⁻¹). Our results displayed that PEMPs intoxication escalated the levels of urea, KIM-1, creatinine and NGAL while reducing the creatinine clearance level. Besides reduction in the activities of GPx, GST, HO-1, CAT, GSR &amp; upsurge in the levels of MDA and ROS were detected in PEMPs group. Conversely, the levels of inflammatory markers including COX-2, IL-6, IL-1β, NF-kB and TNF-α were augmented following the PEMPs intoxication. Besides, the results of the current research demonstrated that the expressions of Bax and caspase-3 were esclated whereas the Bcl-2 expression was lowered from its standard value due to PEMPs provision. However, PON treatment significantly restored the PEMPs-induced aforementioned impairments. Therefore, PON could be used as a therapeutic compound to ameliorate PEMPs-induced kidney impairments in rats, possibly due to its tremendous pharmacotherapeutic potential.</div></div>","PeriodicalId":16205,"journal":{"name":"Journal of King Saud University - Science","volume":"36 11","pages":"Article 103486"},"PeriodicalIF":3.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142534691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nickel toxicology testing in alternative specimen from farm ruminants in a urban polluted environment","authors":"Zafar Iqbal Khan ,&nbsp;Fatima Ghulam Muhammad ,&nbsp;Kafeel Ahmad ,&nbsp;Mona S. Alwahibi ,&nbsp;Hsi-Hsien Yang ,&nbsp;Muhammad Ishfaq ,&nbsp;Sumaira Anjum ,&nbsp;Kishwar Ali ,&nbsp;Khalid Iqbal ,&nbsp;Emanuele Radicetti ,&nbsp;Muhammad Iftikhar Hussain","doi":"10.1016/j.jksus.2024.103520","DOIUrl":"10.1016/j.jksus.2024.103520","url":null,"abstract":"<div><div>The impact of nickel (Ni) metal toxicity, on public health was assessed analyzing forage samples (<em>Acacia nilotica, Zea</em> mays<em>, Pennisetum glaucum, Capparis decidua and Medicago sativa</em>), soils and blood samples of cow, buffalo and sheep (blood plasma, fecal, and hair) collected from three different agro-ecological zones and analyzed through atomic absorption spectrophotometer. Results showed that nickel values differed in soil samples ranging from 4.49 to 9 to 25 mg/kg, in forages from 3.78 to 9.53 mg/kg and in animal samples from 0.65 to 2.42 mg/kg. Nickel concentration, in soil and forage samples, was below the permitted limits. Soil with the minimum nickel level was found under <em>C. decidua</em> while the maximum concentration was reached under the <em>A. nilotica</em>. Among the animals, nickel was maximum in buffaloes that grazed on the Z. mays fodder. Ni was more accumulated in feces than other body tissues. The sheep and buffaloes showed high vulnerability to Ni pollution due to the highest contamination levels at site II and III. Bioconcentration factor, pollution load index and enrichment factor were found to be higher in buffaloes than cows, respectively. The daily intake and health risk index ranged from 0.0056 to 0.0184 mg/kg/day and 0.186–0.614 mg/kg/day respectively. In short, the results of this study evidenced that Nickel-containing fertilizers should never not be used to grow forage species. Government should to lessen the toxic metal accessibility to animals. Although general values were lower than the admitted limit, nickel can be accumulated and the consume of food containing nickel can increase health risks. General monitoring of soil and vegetation pollution load, as well as the use of other non-conventional water like canal water for forage irrigation could be a sustainable solution to decrease the access of nickel in the food chain.</div></div>","PeriodicalId":16205,"journal":{"name":"Journal of King Saud University - Science","volume":"36 11","pages":"Article 103520"},"PeriodicalIF":3.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142658696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic and haplotype diversity of redbreast tilapia (Coptodon rendalli) based on cytochrome oxidase subunit I and D-loop","authors":"Mehrnoush Aminisarteshnizi,&nbsp;Ngonidzashe A.G. Moyo,&nbsp;Mokgadi E. Raphalo","doi":"10.1016/j.jksus.2024.103585","DOIUrl":"10.1016/j.jksus.2024.103585","url":null,"abstract":"<div><div>The genetic diversity in Redbreast tilapia (<em>Coptodon rendalli</em>) is under threat from climate change, human activities, and natural hybridisation, prompting conservation concerns. To investigate the genetic characteristics of <em>C. rendalli</em>, genetic diversity, genetic structure, population differentiation, and historical demography were evaluated using mitochondrial DNA COI (<em>cytochrome oxidase subunit I</em>) and D-loop sequences from various countries. Phylogenetic and genetic diversity analysis based on the mtDNA sequences (COI H = 9, haplogroup = 3, and D-loop H = 5, haplogroup = 3) showed private haplotypes in some of the populations. The results showed significant differences in genetic diversity among the analysed populations (P &lt; 0.001). Molecular variance analysis showed low variation levels among populations (COIF_ST = 0.02176, D-loopF_ST = 0.52), which was significant only for D-loop. The neutrality tests and mismatch distribution based on the mtDNA supported the hypothesis of under-purifying selection of <em>C. rendalli</em>. The results indicated that the population did not undergo expansion. The findings of this study revealed molecular divergence among the <em>C. rendalli</em> populations and may serve as pivot information for the genetic improvement of this species. In conclusion, this study has elucidated the utility of two reliable and cost-effective molecular markers for analysing genetic diversity. Notably, the mitochondrial D-loop region’s sensitivity to reflecting genetic diversity in <em>C. rendalli</em> surpasses that of the COI gene.</div></div>","PeriodicalId":16205,"journal":{"name":"Journal of King Saud University - Science","volume":"36 11","pages":"Article 103585"},"PeriodicalIF":3.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143153533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}