Journal of Investigative Surgery最新文献

{"title":"Radical Resection of Differentiated Thyroid Cancer in Elderly Patients: Evaluation of the Efficacy of the Immunocolloidal Gold Strip Method Combined with Nanocarbon Negative Imaging Tracing Technology for Parathyroid Gland Imaging.","authors":"YanBin Liu, LiJuan Zuo, YunChao Xin, YaChao Liu, ZeDong Tian, XiaoLing Shang","doi":"10.1080/08941939.2024.2447850","DOIUrl":"10.1080/08941939.2024.2447850","url":null,"abstract":"<p><strong>Objective: </strong>Extant imaging methods used for the proper identification of the parathyroid glands to prevent post-operative hypothyroidism associated with the resection of differentiated thyroid cancer (DTC) are limited by factors such as low specificity, high cost, and technical complexity. This study, therefore, sought to investigate the efficacy of the immunocolloidal gold strip method combined with nanocarbon negative imaging tracing technology for parathyroid gland imaging during radical resection of DTC in elderly patients.</p><p><strong>Methods: </strong>A total of 100 elderly patients with DTC were enrolled and randomly divided into two groups: the control group and the observation group. The control group underwent conventional radical thyroidectomy with bilateral cervical lymph node dissection, while the observation group received the immunocolloidal gold strip method combined with nanocarbon negative imaging tracing technology for parathyroid gland imaging during the surgery. The baseline characteristics, intraoperative findings, postoperative parathyroid hormone (PTH), and serum calcium levels, as well as postoperative complications, were compared between the two groups.</p><p><strong>Results: </strong>There were no significant differences in age, gender, body mass index, comorbidities, or smoking history between the two groups. The observation group had a significantly higher number of parathyroid glands identified during surgery compared with the control group. The postoperative PTH and serum calcium levels at postoperative days 1 and 3 and at 6 months were significantly higher in the observation group than those in the control group. The incidence of postoperative hypoparathyroidism was significantly lower in the observation group.</p><p><strong>Conclusion: </strong>The immunocolloidal gold strip method combined with nanocarbon negative imaging tracing technology is effective in identifying and preserving parathyroid glands during radical resection of DTC in elderly patients.</p>","PeriodicalId":16200,"journal":{"name":"Journal of Investigative Surgery","volume":"38 1","pages":"2447850"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142978898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neutrophil-to-Hemoglobin Ratio and Monocyte-to-Hemoglobin Ratio Predict Prolonged Length of Stay in Patients Undergoing Surgery for Pelvic or Acetabular Fractures.","authors":"Wei-Gang Pan, Yu-Ching Chou, Tsu-Te Yeh","doi":"10.1080/08941939.2025.2515067","DOIUrl":"https://doi.org/10.1080/08941939.2025.2515067","url":null,"abstract":"<p><strong>Objective: </strong>This study evaluated the neutrophil-to-hemoglobin ratio (NHR) and monocyte-to-hemoglobin ratio (MHR) as predictors of prolonged hospital stay in patients undergoing surgery for pelvic or acetabular fractures.</p><p><strong>Methods: </strong>We retrospectively reviewed records of patients aged ≥ 18 years who underwent open reduction and internal fixation (ORIF). NHR and MHR were calculated from blood samples at admission and 24 hours postoperatively. Prolonged hospital length of stay (LOS) was defined as ≥ 25 days (75th percentile). Optimal cutoffs were determined by ROC curve analysis. Multivariable logistic regression identified associations between clinical variables and prolonged LOS.</p><p><strong>Results: </strong>Among 172 patients (mean age 44.5), postoperative NHR > 0.46 and MHR > 0.02 were independently associated with prolonged LOS in patients with acetabular fractures (aOR = 5.90 and 5.42, respectively). No significant associations were found in pelvic fractures.</p><p><strong>Conclusions: </strong>Elevated postoperative NHR and MHR are strongly associated with prolonged LOS in patients with acetabular fractures and may assist in perioperative risk stratification and clinical decision-making.</p>","PeriodicalId":16200,"journal":{"name":"Journal of Investigative Surgery","volume":"38 1","pages":"2515067"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144317124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Safety of Primary Anastomosis Without Protective Stoma in Emergency Left Colon Surgery: A Meta-Analysis.","authors":"Zhiyan Wang, Wentao Sheng, Senjie Dai, Xuanzhou Li, Guojian Lin, Xiaohong Kang","doi":"10.1080/08941939.2025.2520992","DOIUrl":"10.1080/08941939.2025.2520992","url":null,"abstract":"<p><strong>Background: </strong>This study aims to evaluate the safety of primary anastomosis (PA) without a protective stoma in emergency left colon surgery.</p><p><strong>Methods: </strong>A systematic search was conducted in the PubMed, Web of Science, Embase, and Cochrane Library databases, covering articles from the inception of these databases until September 2024. The primary outcome was the incidence of anastomotic leakage.</p><p><strong>Results: </strong>The PA group exhibited a higher incidence of anastomotic leakage compared to the stoma surgery group (odds ratios (OR) = 5.86, <i>p</i> = 0.05). However, in cases of perforated diverticulitis (OR = 3.80, <i>p</i> = 0.19) and malignant obstruction (OR = 5.40, <i>p</i> = 0.23), the PA group did not show an increased risk of anastomotic leakage. In terms of other outcomes, the reoperation rate in the PA group was higher compared to the stoma surgery group (OR = 1.89, <i>p</i> < 0.001). However, there were no statistically significant differences in the mortality rate (OR = 1.04, <i>p</i> = 0.80) or the incidence of postoperative complications (OR = 1.50, <i>p</i> = 0.27) between the two groups.</p><p><strong>Conclusion: </strong>Primary anastomosis without a protective stoma is generally safe and can be considered a viable option in emergency left colon surgery.</p>","PeriodicalId":16200,"journal":{"name":"Journal of Investigative Surgery","volume":"38 1","pages":"2520992"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144475584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Impact of Screw Density on the Outcomes of Adolescent Idiopathic Scoliosis Correction Surgery: A Multicenter Retrospective Study.","authors":"ZhaoJun Lu, Chong Liu, JiaKun Li, XiaoPeng Qin, Jiarui Chen, Jiang Xue, Hao Li, Tianyou Chen, Tao Chen, XinLi Zhan","doi":"10.1080/08941939.2025.2531589","DOIUrl":"https://doi.org/10.1080/08941939.2025.2531589","url":null,"abstract":"<p><strong>Background: </strong>To evaluate the efficacy of low screw density constructs versus high screw density constructs in adolescent idiopathic scoliosis (AIS) surgery.</p><p><strong>Methods: </strong>Data were collected from AIS patients who underwent pedicle screw fixation surgery at two medical centers. Patients were stratified into low and high screw density groups, calculated as pedicle screws per fused vertebral level. The dataset comprised demographics, radiological parameters, surgical outcomes and postoperative complications.</p><p><strong>Results: </strong>Of 213 AIS patients analyzed, 114 and 99 comprised low- and high-density groups respectively. Compared with high-density constructs, low-density constructs demonstrated shorter operative time (median: 17.93 min, IQR: 14.66-20.50 vs. 22.15 min, IQR: 16.80-24.77; <i>p</i> = 6.30e-07), lower intraoperative blood loss (median: 762.29 ml, IQR: 600-900 vs. 954.19 ml, IQR: 800-1030; <i>p</i> = 7.70e-07), fewer postoperative pain (median: 4.719, IQR: 3-7 vs. 5.505, IQR: 3.5-7; <i>p</i> = 0.009), and shorter hospital stays (median: 11.15 days, IQR: 7-13.5 vs. 12.48 days, IQR: 8-15; <i>p</i> = 0.04). Both groups had equivalent Cobb angle correction (median: 67.31%, IQR: 60.50% -76.84% vs. 67.97%, IQR: 62.54%-73.52%; <i>p</i> = 0.90).</p><p><strong>Conclusion: </strong>Optimizing screw density may minimize intraoperative blood loss and postoperative pain without affecting correction efficacy in AIS; however, longitudinal studies are needed to assess long-term functional and quality-of-life outcomes.</p>","PeriodicalId":16200,"journal":{"name":"Journal of Investigative Surgery","volume":"38 1","pages":"2531589"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144707771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Prior Bariatric Surgery on the Outcomes of Joint Arthroplasty: A Systematic Review and Meta-Analysis.","authors":"Zhou Zhang, Xiang Shi, Wei Liu, Jianwei Wang, Qingfeng Shen, Guozhu Xu, Jiakuan Bao, Yupeng Dong","doi":"10.1080/08941939.2024.2446579","DOIUrl":"10.1080/08941939.2024.2446579","url":null,"abstract":"<p><strong>Objective: </strong>Obesity is a risk factor for joint arthroplasty complications. With this systematic review and meta-analysis, we assessed whether a positive history of bariatric surgery influences postoperative outcomes in patients undergoing various types of joint arthroplasty.</p><p><strong>Methods: </strong>We conducted a comprehensive search database such as Scopus, PubMed, Medline Ovid, CNKI, and CENTRAL for studies comparing outcomes between patients undergoing arthroplasty with and without a history of bariatric surgery. We extracted data on short-term medical complications, venous thromboembolism (VTE), periprosthetic infections, superficial wound infections, hospital stay length, and operative time. We used the data to conduct meta-analyses using random-effects models and subgroup analyses based on the type of arthroplasty.</p><p><strong>Results: </strong>Overall, 15 studies with nearly 150,000 participants were included. The analysis showed that prior bariatric surgery did not significantly affect overall medical complications post-arthroplasty, with OR of 0.968 (95%CI, 0.706-1.327). For VTE, the results similarly indicated no substantial difference, with an OR of 0.912 (95%CI, 0.644-1.291). In assessing periprosthetic infections, the OR was 0.754 (95%CI, 0.535-1.064), showing comparable rates between patients with and without a history of bariatric surgery.Regarding superficial wound infections, the analysis produced an OR of 2.390 (95%CI, 0.723-7.897), indicating variability but not statistical significance. Hospital stay length was reduced in patients with a history of bariatric surgery, reflected by SMD of -0.113 (95%CI, -0.221 to -0.005). Lastly, operative time also showed a significant reduction, with an SMD of -0.462 (95%CI, -0.865 to -0.059).</p><p><strong>Conclusions: </strong>Prior bariatric surgery does not reduce the risk of short-term complications post-arthroplasty, but it seems to reduce the hospital stay length and operative time. The effects vary significantly across different types of joint arthroplasty, suggesting a need for tailored preoperative assessments and care protocols.</p><p><strong>Protocol registration: </strong>This systematic review and meta-analysis was registered at PROSPERO, with the number: CRD42024539052.</p>","PeriodicalId":16200,"journal":{"name":"Journal of Investigative Surgery","volume":"38 1","pages":"2446579"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142950210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness Analysis of Sevoflurane Combined with Propofol or Remimazolam Anesthesia in Pediatric Laparoscopic Inguinal Hernia Repair and Its Impact on Hemodynamics and Postoperative Pain.","authors":"Renqi Liu, Zhiyi Wang, Yuanyuan Meng, Weiye Chen, Min Zhou","doi":"10.1080/08941939.2025.2500438","DOIUrl":"10.1080/08941939.2025.2500438","url":null,"abstract":"<p><strong>Purpose: </strong>This study evaluated the clinical effectiveness of combined treatment of sevoflurane with propofol or remimazolam in decreasing hemodynamic changes and postoperative pain in pediatric patients undergoing laparoscopic inguinal hernia repair (LIHR).</p><p><strong>Methods: </strong>This study prospectively recruited 287 children with hernia admitted to our hospital from September 2020 to October 2023. These children were allocated into the sevoflurane plus remimazolam and sevoflurane plus propofol groups using the random number table method. General clinical data at admission were collected. Clinical outcomes were compared between the two groups. The primary outcomes included anesthesia quality, intraoperative hemodynamic indicators at different time points (MAP, SpO₂, HR), and the postoperative FLACC score, while the secondary outcomes included the PAED score of the children and the incidence of postoperative adverse reactions.</p><p><strong>Results: </strong>No remarkable difference occurred between these two groups regarding general clinical data. Compared to the sevoflurane plus propofol group, the sevoflurane plus remimazolam group resulted in significantly lower MAP and HR at T1, T2, T3, and T4 (<i>p</i> < .05), shorter postoperative awakening time (mean difference: 2.9 min, <i>p</i> < .01), lower FLACC pain scores at 0.5, 1, and 2 h postoperatively (<i>p</i> < .05), and lower PAED scores at 5, 15, and 30 min postoperatively (<i>p</i> < .05). Adverse effects were lower in the sevoflurane + remimazolam group (5% vs. 12%, <i>p</i> = .076).</p><p><strong>Conclusion: </strong>Sevoflurane plus remimazolam in children receiving LIHR has minimal impact on hemodynamics, reduces pain levels within 4 h postoperatively, lowers PAED and agitation scores, and demonstrates high safety.</p>","PeriodicalId":16200,"journal":{"name":"Journal of Investigative Surgery","volume":"38 1","pages":"2500438"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144284941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Epidemiological Study on Survival in Lymphoma of Mucosa-Associated Lymphoid Tissue: A Comparative Analysis by Primary Tumor Location.","authors":"Gui-Ping Chen, Zhen-Zhen Lu, Guan-Zhong Lu, Yi Zhou, San-Gang Wu","doi":"10.1080/08941939.2025.2523858","DOIUrl":"https://doi.org/10.1080/08941939.2025.2523858","url":null,"abstract":"<p><strong>Purpose: </strong>To investigate outcome and local treatment strategy for extranodal mucosa-associated lymphoid tissue (MALT) lymphoma patients.</p><p><strong>Methods: </strong>Retrospectively collected patients using the data from the Surveillance, Epidemiology, and End Results database, including tumor sites with over 200 cases.</p><p><strong>Results: </strong>The cohort comprised 3842 patients, with the gastric region being the most prevalent site (34.9%), followed by salivary gland (16.8%), cutaneous (16.5%), pulmonary (13.3%), intestinal (13.2%), and head and neck (5.4%). The 10-year cancer-specific survival (CSS) was 88.1%, 92.6%, 86.5%, 87.7%, 89.7%, and 97.7% in patients with tumors located in the gastric, salivary gland, pulmonary, head and neck, intestinal, and cutaneous, respectively (<i>p</i> < 0.001). Compared to gastric MALT lymphoma, the cutaneous site exhibited superior CSS (<i>p</i> < 0.001), whereas other sites showed comparable outcomes (all <i>p</i> ≥ 0.05). In gastric MALT lymphoma cases, radiotherapy was associated with better CSS than surgery alone (<i>p</i> < 0.001), while no significant difference was observed between surgery plus radiotherapy versus surgery alone (<i>p</i> = 0.561). Treatment outcomes were similar across the three therapeutic modalities for non-gastric sites.</p><p><strong>Conclusions: </strong>Our findings underscore the biological and clinical heterogeneity of extranodal MALT lymphoma. While localized treatments provide equivalent results across most non-gastric sites, radiotherapy is recommended as the primary noninvasive treatment option.</p>","PeriodicalId":16200,"journal":{"name":"Journal of Investigative Surgery","volume":"38 1","pages":"2523858"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Co-Administration of Midazolam and Dexmedetomidine on Haemodynamics and Stress Response in Elderly Patients with Non-Small Cell Lung Cancer.","authors":"Yanjun Zhao, Dongjiao An, Liang Bi","doi":"10.1080/08941939.2024.2445587","DOIUrl":"https://doi.org/10.1080/08941939.2024.2445587","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objective: &lt;/strong&gt;This study aimed to evaluate the effect of co-administration of midazolam and dexmedetomidine on hemodynamics and stress response in elderly patients with non-small cell lung cancer (NSCLC).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;In this prospective, randomized controlled trial, 154 elderly NSCLC patients scheduled for lobectomy in our oncology department from January 2019 to December 2021 were recruited. Patients were randomized 1:1 to receive either dexmedetomidine (control group) or dexmedetomidine plus midazolam (study group) for anesthesia during lobectomy &lt;i&gt;via&lt;/i&gt; the random number table method, with 77 patients in each group. Perioperative indicators, hemodynamics, and stress reactions of the patients were recorded and compared between the two groups to investigate the efficacy of the two different anesthetic protocols.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;No significant differences were observed between the two groups in terms of operative time, anesthesia time, and intraoperative bleeding volume (&lt;i&gt;p&lt;/i&gt; &gt; 0.05). Preoperative pain, pain at anesthesia recovery, and pain levels 7 days postoperatively were also comparable between the two groups. In the study group, the awakening time was 15 ± 2 min significantly shorter compared to the control group (25 ± 3 min). Cooperation within the first hour was significantly faster by 8.5 ± 0.5 min compared to 6.0 ± 1.0 min in the control group (&lt;i&gt;p&lt;/i&gt; &lt; 0.05). The cost of materials used was significantly higher in the study group, with an average of 300 ± 25 USD, compared to 200 ± 20 USD in the control group (&lt;i&gt;p&lt;/i&gt; &lt; 0.05). Additionally, the two groups showed no significant difference in the need for experience and surveillance (&lt;i&gt;p&lt;/i&gt; &gt; 0.05). Significantly lower visual analog scale (VAS) scores were found one day after the surgery in patients given dexmedetomidine plus midazolam than those anesthetized administered with dexmedetomidine only, suggesting an enhanced pain mitigation effect after incorporating midazolam for anesthetic induction. Patients treated with dexmedetomidine plus midazolam presented with a more stable hemodynamic status than those treated with dexmedetomidine only, as evidenced by the significantly lower variability of mean arterial pressure (MAP), oxygen saturation (SpO2), and heart rate (HR). Co-administration of dexmedetomidine plus midazolam for lobectomy anesthesia resulted in significantly lower serum cortisol (Cor) and norepinephrine (NE) concentrations in patients at anesthesia recovery than dexmedetomidine alone. However, this difference was not observed one day postoperatively. There was no statistically significant difference in the incidence of adverse reactions between the two groups.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusion: &lt;/strong&gt;The combination of midazolam with dexmedetomidine anesthesia in lobectomy improves the intraoperative hemodynamic status of elderly patients with NSCLC and mitigates their stress response. However, further research is req","PeriodicalId":16200,"journal":{"name":"Journal of Investigative Surgery","volume":"38 1","pages":"2445587"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142932030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Hsa-miR-4741/LILRB2 on Senescence of Nucleus Pulposus Cells and Their Prognostic Values in Lumbar Disc Herniation.","authors":"Zhendong He, Nan Zheng, Xiu-Quan Guo, Gang-Gang Wang, Mingjian Lin","doi":"10.1080/08941939.2025.2458180","DOIUrl":"10.1080/08941939.2025.2458180","url":null,"abstract":"<p><strong>Background: </strong>The incidence of lumbar disk herniation (LDH) is usually caused by lumbar disk degeneration. Surgery is a common treatment strategy for LDH, but it can recur, resulting in recurrent disk herniation (RDH).</p><p><strong>Purpose: </strong>To explore the predictive value of hsa-miR-4741 and LILRB2 in the prognosis of LDH surgery and the mechanism of nucleus pulposus senescence.</p><p><strong>Method: </strong>The ROC curves of RDH based on hsa-miR-4741 and LILRB2 were constructed to evaluate their predictive values in the prognosis of LDH surgery. Human nucleus pulposus cells (NPC) was treated by TNF-α to construct a cell senescence model, studying the senescence mechanism. Oxidative stress and senescence markers were detected after overexpression of hsa-miR-4741 and LILRB2 to evaluate their effects on the senescence of NPC. Dual luciferase assay and the transfection of hsa-miR-4741 mimics or inhibitor were used to investigate the targeted regulation of it to LILRB2.</p><p><strong>Results: </strong>The combination of hsa-miR-4741 and LILRB2 showed higher accuracy in predicting the outcome of RDH (AUC = 0.9367), compared with a single molecule. Overexpression of hsa-miR-4741 enhanced TNF-α-induced oxidative stress and senescence, while LILRB2 overexpression had the opposite effect. Hsa-miR-4741 mimics attenuated the luciferase activity of NPC transfected with wt-LILRB2 vector and significantly down-regulated LILRB2 expression. In addition, the antioxidant NAC reversed the promotion of hsa-miR-4741 on NPC senescence.</p><p><strong>Conclusion: </strong>The combination of hsa-miR-4741 and LILRB2 was a good predictor of LDH prognosis. Hsa-miR-4741 promoted oxidative stress-induced NPC senescence by negatively regulating LILRB2.</p>","PeriodicalId":16200,"journal":{"name":"Journal of Investigative Surgery","volume":"38 1","pages":"2458180"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of miR-143-3p in the Development of Hemorrhoids and Postoperative Wound Healing.","authors":"Qing Liu, Wei Han, Ling Wang, Weifang Shang, Xinyu Cao","doi":"10.1080/08941939.2025.2480799","DOIUrl":"10.1080/08941939.2025.2480799","url":null,"abstract":"<p><strong>Background: </strong>Hemorrhoids refer to a common anorectal disorder that is usually associated with vascular proliferation. The present study investigated the role of miR-143-3p in the development of hemorrhoids and postoperative wound healing, aiming to provide novel ideas for the study of the pathogenesis of hemorrhoids and their clinical treatment.</p><p><strong>Methods: </strong>Hemorrhoid tissues and normal perianal tissues were collected from 42 patients who underwent hemorrhoid surgery. The expressions of miR-143-3p, vascular endothelial markers (CD31, vWF, and VEGFR2), and inflammatory factors (TNF-α, IL-1β, and IL-6) in these tissues were determined using RT-qPCR. The correlation of miR-143-3p with CD31, vWF, and VEGFR2 was analyzed using Pearson's method. The proliferation of HUVEC and HaCaT cells was detected using the CCK-8 assay. The migration of HUVEC and HaCaT cells was detected using Transwell assay. The apoptosis of HUVEC cells was detected using flow cytometry.</p><p><strong>Results: </strong>Reduced expression of miR-143-3p in hemorrhoid tissues was negatively correlated to the mRNA levels of CD31, vWF, and VEGFR2. The mRNA levels of CD31, vWF, and VEGFR2 in the HUVEC cells were reduced after miR-143-3p overexpression. Overexpression of miR-143-3p inhibited the proliferation and migration of HUVEC cells while promoting apoptosis in these cells. Upregulation of miR-143-3p decreased the mRNA levels of TNF-α, IL-1β, and IL-6 in HaCaT cells while promoting cell proliferation and migration in these cells.</p><p><strong>Conclusions: </strong>Downregulation of miR-143-3p was noted in hemorrhoids, which could be linked to the regulation of angiogenesis. MiR-143-3p might have an anti-inflammatory role in postoperative wound healing.</p>","PeriodicalId":16200,"journal":{"name":"Journal of Investigative Surgery","volume":"38 1","pages":"2480799"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143670022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}