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When Scientific Reassessment Fails Citizen Science. Comment on Soldo, A. When Citizen Science Becomes Speculation: Evaluating the Reliability of Lamnid Shark Identification from Photographic Records in the Mediterranean. J. Mar. Sci. Eng. 2026, 14, 173. 当科学重新评估失败时,公民科学。评论Soldo, A.当公民科学成为猜测:评估地中海摄影记录中Lamnid鲨鱼识别的可靠性。3 .科学工程学报,2026,14,173。
IF 3.2 3区 地球科学
Journal of Marine Science and Engineering Pub Date : 2026-06-23 DOI: 10.3390/jmse14131148
Patrick L Jambura, Julia Türtscher, Joshua K Moyer, Kenshu Shimada, Ilija Ćetković, Branko Dragičević, Pero Ugarković, Mišo Pavičić, Phillip C Sternes, Simone Niedermüller, Jacopo Bernardi, Matteo Barbato, Frederik H Mollen, Jürgen Kriwet, Douglas J Long, Taketeru Tomita, Charlie J Underwood, Clinton Duffy, Craig P O'Connell, Alessandro De Maddalena, John Chisholm, Gregory B Skomal
{"title":"When Scientific Reassessment Fails Citizen Science. Comment on Soldo, A. When Citizen Science Becomes Speculation: Evaluating the Reliability of Lamnid Shark Identification from Photographic Records in the Mediterranean. <i>J. Mar. Sci. Eng</i>. 2026, <i>14</i>, 173.","authors":"Patrick L Jambura, Julia Türtscher, Joshua K Moyer, Kenshu Shimada, Ilija Ćetković, Branko Dragičević, Pero Ugarković, Mišo Pavičić, Phillip C Sternes, Simone Niedermüller, Jacopo Bernardi, Matteo Barbato, Frederik H Mollen, Jürgen Kriwet, Douglas J Long, Taketeru Tomita, Charlie J Underwood, Clinton Duffy, Craig P O'Connell, Alessandro De Maddalena, John Chisholm, Gregory B Skomal","doi":"10.3390/jmse14131148","DOIUrl":"10.3390/jmse14131148","url":null,"abstract":"<p><p>Soldo [1] raises concerns about the reliability of citizen science-derived photographic records for identifying lamnid sharks in the Mediterranean Sea. Using a recently published juvenile white shark from Croatian waters [2] as a representative case, the author reinterprets the specimen as a porbeagle shark (<i>Lamna nasus</i>) rather than a white shark (<i>Carcharodon carcharias</i>), based primarily on selected dental characters. Given the critically endangered status of white sharks in the Mediterranean [3] and the limited number of confirmed observations [4,5], occurrences of the species in this region serve as valuable population data points [6,7] and merit careful examination. Species identifications and reinterpretations should therefore be well-supported, which includes verification of source materials and firsthand communication with citizen scientists, and make use of multiple diagnostic characters whenever possible. Here, we re-examined the specimen using all observable external and dental characters and show that it is consistent with the white shark. We further demonstrate that reliance on single, variably expressed traits-particularly dental characters affected by ontogeny and individual variation-can lead to erroneous identifications, and emphasize that rigorous taxonomic identifications should depend on a holistic, integrative assessment of all available morphological (and if available genetic) evidence.</p>","PeriodicalId":16168,"journal":{"name":"Journal of Marine Science and Engineering","volume":"14 13","pages":"1148"},"PeriodicalIF":3.2,"publicationDate":"2026-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7619331/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148697496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Integrated Sail–Hull–Turbine Assessment for Wind Power Generation Ship Using Experiment and CFD 基于实验和CFD的风力发电船风帆-船体-涡轮综合评估
3区 地球科学
Journal of Marine Science and Engineering Pub Date : 2026-01-06 DOI: 10.3390/jmse14020111
Nguyen Trang, Taiga Mitsuyuki, Yoshiaki Hirakawa, Thi Pham-Truong, Shun Yokota
{"title":"Integrated Sail–Hull–Turbine Assessment for Wind Power Generation Ship Using Experiment and CFD","authors":"Nguyen Trang, Taiga Mitsuyuki, Yoshiaki Hirakawa, Thi Pham-Truong, Shun Yokota","doi":"10.3390/jmse14020111","DOIUrl":"https://doi.org/10.3390/jmse14020111","url":null,"abstract":"Wind power generation ships (WPG ships), which combine rigid sails for propulsion and underwater turbines for onboard power generation, have attracted increasing attention as a promising concept for utilizing renewable energy at sea. This study presents an integrated assessment of a WPG ship by combining towing-tank experiments, CFD simulations using ANSYS Fluent, and theoretical analysis to evaluate the coupled performance of sails, hull, and underwater turbines. First, sail thrust and bare-hull resistance were quantified to identify the effective operating-speed range under Beaufort 6–8 wind conditions, and the optimal number of rigid sails was determined. Based on a thrust–resistance balance at a representative rated operating point, two turbine configurations (two and four turbines) were preliminarily sized. The results show that ten rigid sails can provide near-maximum thrust without excessive aerodynamic interference, and the installation of turbines significantly reduces the feasible operating range compared to the bare-hull case. For the two-turbine configuration, a common effective ship-speed range of 6.58–8.0 m/s is obtained, whereas the four-turbine configuration is restricted to 6.58–7.44 m/s due to wake losses, additional appendage drag, and near-free-surface effects. The four-turbine configuration exhibits approximately 30% lower total power output than the two-turbine configuration. These findings demonstrate that an integrated, system-level evaluation is essential for WPG ship design and indicate that the two-turbine configuration offers a more favorable balance between power generation capability and operational flexibility.","PeriodicalId":16168,"journal":{"name":"Journal of Marine Science and Engineering","volume":"14 2","pages":"111-111"},"PeriodicalIF":0.0,"publicationDate":"2026-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2077-1312/14/2/111/pdf?version=1767696898","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147893262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Mechanism of the Effect of FCC Slurry Oil Blending Ratio on the Colloidal Stability and Asphaltene Aggregation Behavior of Low-Sulfur Marine Fuel Oil FCC浆油掺比对低硫船用燃料油胶体稳定性及沥青质聚集行为的影响机理
3区 地球科学
Journal of Marine Science and Engineering Pub Date : 2025-09-04 DOI: 10.3390/jmse13091713
Anyang Li, Shengjun Guo, Jianwen Deng, Hong Chen, Jinxuan Laura Wu, Ru Jiang, Jing Tan, Lihua Cheng, Libo Zhang, Qinzhen Fan
{"title":"The Mechanism of the Effect of FCC Slurry Oil Blending Ratio on the Colloidal Stability and Asphaltene Aggregation Behavior of Low-Sulfur Marine Fuel Oil","authors":"Anyang Li, Shengjun Guo, Jianwen Deng, Hong Chen, Jinxuan Laura Wu, Ru Jiang, Jing Tan, Lihua Cheng, Libo Zhang, Qinzhen Fan","doi":"10.3390/jmse13091713","DOIUrl":"https://doi.org/10.3390/jmse13091713","url":null,"abstract":"Addressing IMO 2020 compliance, this study investigates marine fuel oil production from hydrotreated residues, focusing on mitigating excessive total sediment potential (TSP) caused by over-hydrotreatment. This study systematically investigates the impact of blending ratios of Fluid Catalytic Cracking (FCC) slurry oil with Residue Desulfurization (RDS) heavy oil on TSP, colloidal stability, and asphaltene structure evolution. Techniques such as XRD, SEM, and XPS were employed to analyze the structural changes in asphaltenes during the TSP exceeding process. The results indicate that as the FCC slurry oil blending ratio increases, TSP in the blended oil initially rises and then decreases. The peak TSP value of 0.41% occurs at a 10% FCC slurry oil blending ratio, primarily due to high-saturation hydrocarbons in RDS heavy oil disrupting the colloidal stability of asphaltenes in FCC slurry oil. When the blending ratio reaches 25%, TSP significantly decreases to 0.09%, attributed to the solubilizing effect of high aromatic compounds in the FCC slurry oil on the asphaltenes. The ω(Asp)/ω(Res) ratio mirrors the TSP trend, and the colloidal solubilizing capacity of asphaltenes increases with the blending ratio. Asphaltenes in RDS heavy oil exhibit a spherical structure, whereas those in FCC slurry oil show a layered structure. The precipitated asphaltenes in the blends primarily result from the aggregation of asphaltenes in FCC slurry oil, with heteroatoms (N, S, O) mainly originating from RDS heavy oil asphaltenes. During the early stage of blending, TSP formation is dominated by FCC slurry oil asphaltenes, but increasing the aromatic content in the system can significantly reduce TSP. This work provides theoretical and technical support for optimizing marine fuel blending processes in petrochemical enterprises.","PeriodicalId":16168,"journal":{"name":"Journal of Marine Science and Engineering","volume":"13 9","pages":"1713-1713"},"PeriodicalIF":0.0,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2077-1312/13/9/1713/pdf?version=1756990898","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147333952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Experimental Study on the Reinforcement of Calcareous Sand Using Combined Microbial-Induced Carbonate Precipitation (MICP) and Festuca arundinacea Techniques 微生物诱导碳酸盐沉淀(MICP)与羊茅复合加固钙质砂的试验研究
3区 地球科学
Journal of Marine Science and Engineering Pub Date : 2025-04-29 DOI: 10.3390/jmse13050883
Xiuqiong Deng, Ziyu Wang, Yuchun Qin, Liang Cao, Peng Cao, Xie Yu, Yingqi Xie
{"title":"Experimental Study on the Reinforcement of Calcareous Sand Using Combined Microbial-Induced Carbonate Precipitation (MICP) and Festuca arundinacea Techniques","authors":"Xiuqiong Deng, Ziyu Wang, Yuchun Qin, Liang Cao, Peng Cao, Xie Yu, Yingqi Xie","doi":"10.3390/jmse13050883","DOIUrl":"https://doi.org/10.3390/jmse13050883","url":null,"abstract":"Combining the Microbial-Induced Calcium Carbonate Precipitation (MICP) technique with plants to reinforce calcareous sand slopes on reef islands is expected to achieve both reinforcement and economic benefits. In this study, MICP was combined with Festuca arundinacea (MICP-FA) for calcareous sand reinforcement. Based on water retention and scanning electron microscopy (SEM) tests, the water retention performance and mechanism of MICP-reinforced calcareous sand under different cementation solution concentrations and cementation cycles were analyzed. The growth adaptability of Festuca arundinacea was evaluated under different bacteria solution concentrations, cementation solution concentrations and cementation cycles. The engineering applicability of MICP-FA-reinforced calcareous sand was evaluated by wind erosion tests, and the synergistic reinforcement mechanism was analyzed. The results show that with the increase in the cementation solution concentration and cementation cycles, more calcium carbonate filled and adhered to the calcareous sand particles, significantly improving the water retention performance. MICP-FA can enhance the wind erosion resistance of calcareous sand. This synergistic mechanism lies in the surface cementation effect of MICP and the deep anchoring effect of plant roots. This study provides theoretical basis and technical guidance for engineering applications in calcareous sand areas.","PeriodicalId":16168,"journal":{"name":"Journal of Marine Science and Engineering","volume":"13 5","pages":"883-883"},"PeriodicalIF":0.0,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2077-1312/13/5/883/pdf?version=1745925045","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147895514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Layer-by-Layer Self-Assembly Marine Antifouling Coating of Phenol Absorbed by Polyvinylpyrrolidone Anchored on Stainless Steel Surfaces 聚乙烯吡咯烷酮吸附苯酚在不锈钢表面的自组装船舶防污涂层
3区 地球科学
Journal of Marine Science and Engineering Pub Date : 2025-03-14 DOI: 10.3390/jmse13030568
Zaixiang Zheng, Shutong Wu, Haobo Shu, Qingzhen Han, Pan Cao
{"title":"Layer-by-Layer Self-Assembly Marine Antifouling Coating of Phenol Absorbed by Polyvinylpyrrolidone Anchored on Stainless Steel Surfaces","authors":"Zaixiang Zheng, Shutong Wu, Haobo Shu, Qingzhen Han, Pan Cao","doi":"10.3390/jmse13030568","DOIUrl":"https://doi.org/10.3390/jmse13030568","url":null,"abstract":"Marine biofouling is a major problem that contributes to the failure of man-made marine structures. Conventional marine antifouling coatings that release heavy metal ions for antimicrobial purposes are no longer in line with today’s environmental issues. In this paper, a layer-by-layer (LBL) self-assembled marine antifouling coating based on an addition reaction between polyvinylpyrrolidone (PVP) and phenols to anchor pyrogallic (PG) with an antimicrobial effect on stainless steel surfaces is presented. For this purpose, three phenolics were selected, and their antifouling effects were compared. Field emission scanning electron microscopy, contact angle measurement, X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared spectroscopy analysis (FTIR) were used to thoroughly characterize the LBLPGs, and the results showed superior homogeneity of the coatings with no significant delamination. Simulated marine antifouling and friction tests showed that the coating inhibited Staphylococcus aureus (S. aureus), Escherichia coli (E. coli), and Phaeodactylum tricornutum (P. tricornutum) by more than 90% and reduced the friction coefficient of the stainless steel surface from 0.38 to 0.24, demonstrating superior antifouling and friction resistance effects.","PeriodicalId":16168,"journal":{"name":"Journal of Marine Science and Engineering","volume":"13 3","pages":"568-568"},"PeriodicalIF":0.0,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2077-1312/13/3/568/pdf?version=1741959146","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147331499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Research on Visualization Methods for Marine Environmental Element Fields in Twin Spaces 双空间海洋环境要素场可视化方法研究
3区 地球科学
Journal of Marine Science and Engineering Pub Date : 2025-02-26 DOI: 10.3390/jmse13030449
Lianwei Li, Shiyu Wu, Cunjin Xue, Yingying Ma, Qunce Qin
{"title":"Research on Visualization Methods for Marine Environmental Element Fields in Twin Spaces","authors":"Lianwei Li, Shiyu Wu, Cunjin Xue, Yingying Ma, Qunce Qin","doi":"10.3390/jmse13030449","DOIUrl":"https://doi.org/10.3390/jmse13030449","url":null,"abstract":"Visualization of marine environmental field elements is one of the core technologies in marine science research. Particularly in the context of “digital twin of the ocean” (DTO) construction and application, accurately reproducing the dynamic evolution of marine environmental elements remains a critical challenge. Existing visualization methods are primarily limited to static displays and fail to achieve deep integration and dynamic expression of marine environmental elements and sea conditions. To address this, this paper proposes a new visualization method for marine environmental element fields in a twin-space framework. This method first constructs dynamic wave twin objects based on wave data, then develops a marine environmental element field model based on environmental element data, and finally generates a coupled marine environmental element twin object through integration. This approach facilitates the dynamic and collaborative visualization of multiple marine elements. The collaborative visualization results of wave information, ocean surface temperature, and salinity data indicate that this method effectively enhances the dynamic expression capability and information fusion level of the ocean environment feature field. It provides new ideas and approaches for the development of ocean environment visualization technology in twin spaces, with promising application potential.","PeriodicalId":16168,"journal":{"name":"Journal of Marine Science and Engineering","volume":"13 3","pages":"449-449"},"PeriodicalIF":0.0,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2077-1312/13/3/449/pdf?version=1740645649","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147922006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Experimental and Numerical Study on the Seakeeping Performance of a Wind-Powered Generation Ship Considering Boom Configurations 考虑臂架结构的风力发电船耐浪性能试验与数值研究
3区 地球科学
Journal of Marine Science and Engineering Pub Date : 2025-02-22 DOI: 10.3390/jmse13030412
Nguyen Trang, Taiga Mitsuyuki, Yoshiaki Hirakawa, Katsuaki Sadakata, Yuki Nakamura
{"title":"Experimental and Numerical Study on the Seakeeping Performance of a Wind-Powered Generation Ship Considering Boom Configurations","authors":"Nguyen Trang, Taiga Mitsuyuki, Yoshiaki Hirakawa, Katsuaki Sadakata, Yuki Nakamura","doi":"10.3390/jmse13030412","DOIUrl":"https://doi.org/10.3390/jmse13030412","url":null,"abstract":"This study investigates the seakeeping performance of a wind power generation ship (WPG ship). This type of vessel uses rigid sails for propulsion and submerged turbines in the form of either two or four booms to generate energy. The research includes both tank tests and simulations using Ansys AQWA, validated with the new strip method (NSM). The vessel used in this study is the container ship KCS. Overall, the power generator increases the ship’s stability and reduces roll but has almost no impact on pitch. The findings show that the 4-boom configuration offers better stability and seakeeping than the 2-boom configuration. The ship’s speed has a significant impact on the ship’s RAO, especially roll and pitch, both for the bare hull and the hull with power generation equipment. When the ship’s speed increases slightly, the roll RAO tends to decrease, but as the speed becomes higher, the RAO tends to increase. Wind conditions notably increase the roll RAO peak, reducing stability, while pitch changes are minimal. The KCS model maintains operational capability in winds up to Beaufort scale 11.","PeriodicalId":16168,"journal":{"name":"Journal of Marine Science and Engineering","volume":"13 3","pages":"412-412"},"PeriodicalIF":0.0,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2077-1312/13/3/412/pdf?version=1740222825","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147908550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
RETRACTED: Wang et al. Amplitude and Phase Computable Ocean Wave Real-Time Modeling with GPU Acceleration. J. Mar. Sci. Eng. 2022, 10, 1208 撤稿:Wang et al。基于GPU加速的振幅和相位可计算海浪实时建模。3 .科学工程学报,2022,10,1208
3区 地球科学
Journal of Marine Science and Engineering Pub Date : 2025-01-22 DOI: 10.3390/jmse13020200
Guigui Wang, Shihan Tan, Ge Song, Sheng Wang
{"title":"RETRACTED: Wang et al. Amplitude and Phase Computable Ocean Wave Real-Time Modeling with GPU Acceleration. J. Mar. Sci. Eng. 2022, 10, 1208","authors":"Guigui Wang, Shihan Tan, Ge Song, Sheng Wang","doi":"10.3390/jmse13020200","DOIUrl":"https://doi.org/10.3390/jmse13020200","url":null,"abstract":"The journal retracts the article titled “Amplitude and Phase Computable Ocean Wave Real-Time Modeling with GPU Acceleration” [...]","PeriodicalId":16168,"journal":{"name":"Journal of Marine Science and Engineering","volume":"13 2","pages":"200-200"},"PeriodicalIF":0.0,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2077-1312/13/2/200/pdf?version=1737540267","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147910744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Crude Oil Biodegradation by a Biosurfactant-Producing Bacterial Consortium in High-Salinity Soil 高盐度土壤中产生物表面活性剂菌群对原油的生物降解作用
3区 地球科学
Journal of Marine Science and Engineering Pub Date : 2024-11-10 DOI: 10.3390/jmse12112033
Weiwei Chen, Jiawei Sun, Ru‐Rong Ji, Jun Min, Luyao Wang, Jiawen Zhang, Hongjin Qiao, Shiwei Cheng
{"title":"Crude Oil Biodegradation by a Biosurfactant-Producing Bacterial Consortium in High-Salinity Soil","authors":"Weiwei Chen, Jiawei Sun, Ru‐Rong Ji, Jun Min, Luyao Wang, Jiawen Zhang, Hongjin Qiao, Shiwei Cheng","doi":"10.3390/jmse12112033","DOIUrl":"https://doi.org/10.3390/jmse12112033","url":null,"abstract":"Bioremediation is a promising strategy to remove crude oil contaminants. However, limited studies explored the potential of bacterial consortia on crude oil biodegradation in high salinity soil. In this study, four halotolerant strains (Pseudoxanthomonas sp. S1-2, Bacillus sp. S2-A, Dietzia sp. CN-3, and Acinetobacter sp. HC8-3S), with strong environmental tolerance (temperature, pH, and salinity), distinctive crude oil degradation, and beneficial biosurfactant production, were combined to construct a bacterial consortium. The inoculation of the consortium successfully degraded 97.1% of total petroleum hydrocarbons in 10 days, with notable removal of alkanes, cycloalkanes, branched alkanes, and aromatic hydrocarbons. Functional optimization showed that this consortium degraded crude oil effectively in a broad range of temperature (20–37 °C), pH (6–9), and salinity (0–100 g/L). In salt-enriched crude-oil-contaminated soil microcosms, the simultaneous treatment of bioaugmentation and biostimulation achieved the highest crude oil degradation rate of 568.6 mg/kg/d, compared to treatments involving abiotic factors, natural attenuation, biostimulation, and bioaugmentation after 60 days. Real-time PCR targeting the 16S rRNA and alkB genes showed the good adaptability and stability of this consortium. The degradation property of the constructed bacterial consortium and the engineered consortium strategy may have potential use in the bioremediation of crude oil pollution in high-salinity soil.","PeriodicalId":16168,"journal":{"name":"Journal of Marine Science and Engineering","volume":"12 11","pages":"2033-2033"},"PeriodicalIF":0.0,"publicationDate":"2024-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147901335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
TLR2/TLR5 Signaling and Gut Microbiota Mediate Soybean-Meal-Induced Enteritis and Declined Growth and Antioxidant Capabilities in Large Yellow Croaker (Larimichthys crocea) TLR2/TLR5信号和肠道菌群介导豆粕诱导的大黄鱼肠炎、生长和抗氧化能力下降
3区 地球科学
Journal of Marine Science and Engineering Pub Date : 2024-11-08 DOI: 10.3390/jmse12112016
Lei Zheng, Chao Zeng, Wanqin Zhu, Jiaonan Zhang, Lei Wang, Jianchun Shao, Wei Zhao
{"title":"TLR2/TLR5 Signaling and Gut Microbiota Mediate Soybean-Meal-Induced Enteritis and Declined Growth and Antioxidant Capabilities in Large Yellow Croaker (Larimichthys crocea)","authors":"Lei Zheng, Chao Zeng, Wanqin Zhu, Jiaonan Zhang, Lei Wang, Jianchun Shao, Wei Zhao","doi":"10.3390/jmse12112016","DOIUrl":"https://doi.org/10.3390/jmse12112016","url":null,"abstract":"Soybean meal, renowned for its high yield, cost efficiency, and protein richness, serves as a pivotal plant-based alternative to fish meal. However, high soybean meal inclusion in Larimichthys crocea diets is linked to enteritis and oxidative damage, with unknown mechanisms. Our study aims to elucidate the molecular basis of soybean-meal-induced enteritis and its impact on intestinal microbiota in L. crocea. To this end, four isonitrogenous and isolipidic diets with varying soybean meal levels (0% FM, 15% SBM15, 30% SBM30, and 45% SBM45) were administered to L. crocea for 8 weeks. The results indicated that the SBM30 and SBM45 treatments significantly hindered fish growth, digestive efficiency, and protein utilization. Furthermore, high soybean meal levels (SBM30 and SBM45) activated intestinal Toll-like receptors (TLR2A, TLR2B, TLR5, and TLR22), stimulating C-Rel and mTOR protein expression and elevating ERK phosphorylation. This led to increased pro-inflammatory cytokine production (IL-1β, IL-6, and TNF-α) and decreased anti-inflammatory cytokine expression (IL-4/13A, IL-4/13B, and TGF-β), suggesting a potential signaling pathway for soybean-meal-induced enteritis. Furthermore, enteritis induced by high soybean meal levels led to oxidative damage, evident from increased MDA levels and decreased antioxidant enzyme activities (SOD and CAT). The SBM30 and SBM45 treatments increased Firmicutes and Bacteroidetes abundance in fish gut microbiota, while Proteobacteria abundance decreased. This microbiota shift may enhance soybean meal nutrient utilization, yet high soybean meal concentrations still impair growth. A soybean-meal-rich diet promotes harmful bacteria like Rhodococcus and depletes probiotics like Ralstonia, increasing disease risks. L. crocea has limited tolerance for soybean meal, necessitating advanced processing to mitigate anti-nutritional factors. Ultimately, exploring alternative protein sources beyond soybean meal for fish meal replacement is optimal for L. crocea.","PeriodicalId":16168,"journal":{"name":"Journal of Marine Science and Engineering","volume":"12 11","pages":"2016-2016"},"PeriodicalIF":0.0,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2077-1312/12/11/2016/pdf?version=1731065129","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147907761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 10
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