Journal of Investigative Medicine最新文献_第3页

EXPRESS: Challenging Biological Samples and Strategies for DNA Extraction.

IF 2.5 4区医学

Journal of Investigative Medicine Pub Date : 2025-03-03 DOI: 10.1177/10815589251327503

Ajay Kumar Rana

{"title":"EXPRESS: Challenging Biological Samples and Strategies for DNA Extraction.","authors":"Ajay Kumar Rana","doi":"10.1177/10815589251327503","DOIUrl":"10.1177/10815589251327503","url":null,"abstract":"Despite the significant progress of DNA profiling in forensic science, the extraction of DNA from challenging samples remains a formidable obstacle in forensic laboratories. Particularly, hard tissues (bones, teeth, hair, and nails), formalin-fixed tissues, and contaminated samples pose considerable difficulties. DNA extraction from such samples is often complicated, resulting in scanty, degraded, and contaminated DNA. Moreover, the presence of inhibitors from the surrounding environment further hinders DNA quantification and amplification, presenting additional challenges. This review article delves into the molecular basis of these challenges and examines the mechanistic principles underlying standard DNA extraction protocols. To overcome these obstacles, skilled efforts and additional pre-processing techniques are generally required before organic or silica column-based DNA extraction. Such techniques may involve scraping the waste, sample cleaning with detergents, disinfecting, demineralization of bones and teeth, long proteolytic enzyme treatment, in some cases, harsh methods like hot alkali treatment, tailored to the specific sample type. By addressing these challenges and understanding the molecular intricacies involved in DNA extraction, forensic scientists can improve the reliability and success rate of DNA analysis from difficult forensic samples. This review serves as a comprehensive resource for understanding the complexities of DNA extraction and offers potential solutions for recovering the DNA from highly challenging samples.","PeriodicalId":16112,"journal":{"name":"Journal of Investigative Medicine","volume":" ","pages":"10815589251327503"},"PeriodicalIF":2.5,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

EXPRESS: Peripheral ICOS+CD4 T cell frequency: an indicator for short-term progression in resectable advanced gastric cancer following platinum-based chemotherapy. 快讯外周 ICOS+CD4 T 细胞频率：铂类化疗后可切除晚期胃癌短期进展的指标。

IF 2.5 4区医学

Journal of Investigative Medicine Pub Date : 2025-03-02 DOI: 10.1177/10815589251327133

Yu Zhang, Xiaodi Zhao, Zhiling Ma, Peng Wang, Sen Zhang, Jiaxin Li

{"title":"EXPRESS: Peripheral ICOS+CD4 T cell frequency: an indicator for short-term progression in resectable advanced gastric cancer following platinum-based chemotherapy.","authors":"Yu Zhang, Xiaodi Zhao, Zhiling Ma, Peng Wang, Sen Zhang, Jiaxin Li","doi":"10.1177/10815589251327133","DOIUrl":"https://doi.org/10.1177/10815589251327133","url":null,"abstract":"Background: Peripheral frequency of inducible T cell co-stimulator (ICOS)+CD4 T cells is associated with immunotherapy in gastric cancer (GC); however, limited studies have clarified its association with chemotherapy response.Methods: This was a prospective, pilot study. A total of 120 participants with newly diagnosed GC and 50 advanced GC patients were recruited; and their prognosis and survival were assessed. The frequency of ICOS+CD4 T cells was examined using flow cytometry.Results: The frequency of ICOS+CD4 T cells in stage III GC patients was significantly higher than that of other stages patients. In xenograft GC animals, the frequency of ICOS+CD4 in peripheral blood was positively correlated with tumor volume in mice (P=0.0496). High frequency of peripheral ICOS+CD4 was significantly positively correlated with peripheral IFN-γ concentration. Co-culture experiments showed that the presence of GC cells increased the ratio of ICOS+CD4 T cells derived from peripheral blood. The initial peripheral frequency of ICOS+CD4 T cells in the GC progression-group was significantly lower than that in the stable-group after 3 months of platinum-based chemotherapy (P=0.0318).Conclusion: The frequency of ICOS+CD4 can effectively predicts the short-term progression risk for resectable advanced GC under platinum-based chemotherapy.","PeriodicalId":16112,"journal":{"name":"Journal of Investigative Medicine","volume":" ","pages":"10815589251327133"},"PeriodicalIF":2.5,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143537259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of the synergic effect of mocetinostat and capecitabine in a triple-negative breast neoplasms mouse model. EXPRESS：研究莫西他他和卡培他滨在三阴性乳腺肿瘤小鼠模型中的协同作用。

IF 2.5 4区医学

Journal of Investigative Medicine Pub Date : 2025-03-01 Epub Date: 2025-01-14 DOI: 10.1177/10815589241309603

Hacer Kaya Çakir, Onur Eroğlu

{"title":"Investigation of the synergic effect of mocetinostat and capecitabine in a triple-negative breast neoplasms mouse model.","authors":"Hacer Kaya Çakir, Onur Eroğlu","doi":"10.1177/10815589241309603","DOIUrl":"10.1177/10815589241309603","url":null,"abstract":"Combined administration of two or more drugs is emerging as a new strategy in triple-negative breast neoplasms. This is the first study to investigate the combination of the histone deacetylase inhibitor mocetinostat and the antimetabolite drug capecitabine in triple-negative mammary neoplasms in a preclinical mouse model. Thirty-five female mice were grouped into the control group, capecitabine group, mocetinostat group, and combined drugs group. At the end of the experimental period, body weight, and tumor weight were measured and tumor tissue and lung tissue were histologically examined. The results showed that the body weight of mice in the drug-treated groups was reduced by about 18%. Tumor weights were also reduced by 21% in the mocetinostat group, 27.5% in the capecitabine group, and 45% in the combined group. The combination of mocetinostat and capecitabine decreased the formation of tumors and metastases in lung tissue. In summary, the combination of mocetinostat and capecitabine was more effective than either drug alone in reducing the size of triple-negative breast neoplasms in a mouse model.","PeriodicalId":16112,"journal":{"name":"Journal of Investigative Medicine","volume":" ","pages":"320-327"},"PeriodicalIF":2.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142846751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluorouracil enhances the anti-pancreatic cancer effect of anti-PD-L1 antibodies via up-regulating the expression of PD-L1 in cancer cells. EXPRESS：氟尿嘧啶通过上调癌细胞中PD-L1的表达来增强抗PD-L1抗体的抗胰腺癌作用。

IF 2.5 4区医学

Journal of Investigative Medicine Pub Date : 2025-03-01 Epub Date: 2025-01-23 DOI: 10.1177/10815589251314192

Wei Wang, Sujing Zhang, Cong Wang, Siming Gao, Lingling Zhang, Changwang Zhang, Zheng Zheng, Jiancong Zhang, Hui Xu, Changwen Bo, Na Li

{"title":"Fluorouracil enhances the anti-pancreatic cancer effect of anti-PD-L1 antibodies via up-regulating the expression of PD-L1 in cancer cells.","authors":"Wei Wang, Sujing Zhang, Cong Wang, Siming Gao, Lingling Zhang, Changwang Zhang, Zheng Zheng, Jiancong Zhang, Hui Xu, Changwen Bo, Na Li","doi":"10.1177/10815589251314192","DOIUrl":"10.1177/10815589251314192","url":null,"abstract":"Pancreatic cancer is characterized by occult onset, low early diagnosis rate, rapid progress, and poor prognosis. Due to the low response rate and low programmed cell death ligand 1 (PD-L1) expression in pancreatic cancer, the therapeutic application of PL-L1 inhibitors in pancreatic cancer is greatly limited. In vitro studies showed that the expression of PD-L1 increased in pancreatic cancer cells stimulated by fluorouracil (5-FU). We aim to explore the combined effect of 5-FU and anti-PD-L1 antibodies and to provide a reference for the clinical application of PD-L1 antibodies in pancreatic cancer. In the current study, male BALB/c mice were adopted to construct a tumor-bearing model of pancreatic cancer cells. 5-FU and anti-mouse PD-L1 antibodies were combined and administered to evaluate their synergistic effects. The enhancing immune cytotoxicity effect of 5-FU sensitizing the anti-PD-L1 antibody in vivo and in vitro was analyzed by immunohistochemistry and western blot assays. Results showed that 5-FU and anti-PD-L1 antibody combination increased the expression of PD-L1 and IFN-γ, and infiltration of CD8+ T lymphocytes in pancreatic xenograft tumor tissues, which was proven by immunohistochemistry and western analysis. Moreover, the combination with the 5-FU remarkably enhanced the immune cytotoxicity of anti-PD-L1 antibodies in mice. In vitro analysis demonstrates that 5-FU increases the expression of PD-L1 on the surface of pancreatic cancer cell lines via up-regulating nuclear factor kappa B (NF-κB) and Protein kinase B (AKT) pathways. This synergistic effect could be abolished by NF-κB and AKT inhibitors.","PeriodicalId":16112,"journal":{"name":"Journal of Investigative Medicine","volume":" ","pages":"310-319"},"PeriodicalIF":2.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142931958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic efficacy of orthokeratology and 0.01% atropine in controlling pediatric myopia progression: A retrospective analysis. EXPRESS：角膜塑形镜和0.01%阿托品在控制儿童近视进展中的协同作用：回顾性分析。

IF 2.5 4区医学

Journal of Investigative Medicine Pub Date : 2025-03-01 Epub Date: 2025-01-23 DOI: 10.1177/10815589241308819

Yingying Yang, Zhigang Xiao, Jing Ouyang, Yan Guo

引用次数: 0

Pharmacokinetic advantages of lipophilic statins over rosuvastatin in COVID-19 management. EXPRESS：亲脂性他汀类药物在COVID-19治疗中的药代动力学优势优于瑞舒伐他汀。

IF 2.5 4区医学

Journal of Investigative Medicine Pub Date : 2025-03-01 Epub Date: 2025-01-21 DOI: 10.1177/10815589251314193

Chia Siang Kow, Dinesh Sangarran Ramachandram, Syed Shahzad Hasan, Kaeshaelya Thiruchelvam

引用次数: 0

Temporal trends of inflammatory bowel disease: Nationwide study from 2010 to 2020. 快讯炎症性肠病的时间趋势：2010-2020年全国性研究。

IF 2.5 4区医学

Journal of Investigative Medicine Pub Date : 2025-03-01 Epub Date: 2025-02-03 DOI: 10.1177/10815589241300077

Silpa Choday, Anne Jarvis, Peter Kim, Keng-Yu Chuang, Neil Vyas

{"title":"Temporal trends of inflammatory bowel disease: Nationwide study from 2010 to 2020.","authors":"Silpa Choday, Anne Jarvis, Peter Kim, Keng-Yu Chuang, Neil Vyas","doi":"10.1177/10815589241300077","DOIUrl":"10.1177/10815589241300077","url":null,"abstract":"This study examined inflammatory bowel disease (IBD) trends over the last 10 years, including their nationwide patterns, and the outcomes of the utilization of total parental nutrition (TPN). Nationwide inpatient sample (NIS) database from 2010 to 2020, was used to identify IBD hospitalization and discharges and investigate outcomes, including in-hospital mortality and hospital resource utilization. The hospitalizations for IBD combining both Crohn's disease (CD) and ulcerative colitis (UC) initially noted a rising trend until 2016 followed by a decreasing trend with statistical significance (p < 0.001). A decreasing trend in TPN utilization in CD dropped from 6.2 to 5.4% (p = 0.07). The prevalence of malnutrition in CD cases significantly increased from 11.6 to 16.6% (p < 0.001), and the use of TPN in malnutrition cases decreased from 25.0 to 20.0% with statistical significance (p = 0.002). TPN in UC cases also exhibited a downward trend, declining from 5.3 to 3.1% with statistical significance (p < 0.001). However, there was a noteworthy increase in malnutrition rates, rising from 13.5 to 17.3% (p = 0.087). Similarly, the utilization of TPN in malnutrition cases among UC cases displayed a significant decrease from 19.9 to 11.3% (p < 0.001). The combined use of TPN in IBD showed a decreased trend from 5.9 to 4.3% with statistical significance (p < 0.001). There is a decline in the trend in TPN usage in both CD and UC. The hospital costs and malnutrition trend has increased, while the inpatient mortality, length of stay, and TPN use decreased over the year.","PeriodicalId":16112,"journal":{"name":"Journal of Investigative Medicine","volume":" ","pages":"279-289"},"PeriodicalIF":2.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142693118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening for presence of primary Sjogren's syndrome in patients with autoimmune thyroid diseases. EXPRESS：自身免疫性甲状腺疾病患者原发性干燥综合征的筛查

IF 2.5 4区医学

Journal of Investigative Medicine Pub Date : 2025-03-01 Epub Date: 2025-01-09 DOI: 10.1177/10815589241308573

Manar Elgebaly, Wael Farrag, Khaled Shalaby, Hesham Elserougy, Manal Saad Negm

{"title":"Screening for presence of primary Sjogren's syndrome in patients with autoimmune thyroid diseases.","authors":"Manar Elgebaly, Wael Farrag, Khaled Shalaby, Hesham Elserougy, Manal Saad Negm","doi":"10.1177/10815589241308573","DOIUrl":"10.1177/10815589241308573","url":null,"abstract":"Autoimmune thyroid disorders (AITD) are the most common autoimmune human disorders as the thyroid gland is a main target for autoimmunity. The association between rheumatologic and thyroid disorders has long been known, the most common being the association with rheumatoid arthritis. Our study was conducted to screen for the presence of symptoms, signs, and immune markers suggesting the presence of Sjogren's syndrome among patients with autoimmune thyroid disorders. Eighty AITD patients (46 patients with Hashimoto's thyroiditis and 34 patients with Graves' disease) were included in the study and 40 healthy subjects matched age and sex as a control group. The two groups were compared according to 2002 the American-European Consensus Group (AECG) criteria for diagnosis of primary Sjogren's syndrome. 12.5% of AITD patients (n = 10 patients) were diagnosed with Sjogren's syndrome (AITD-SS). Eight out of ten of AITD-SS had Hashimoto's disease while only two had Grave's disease. Anti-Ro was detected in serum of seven patients of the AITD patients with Sjogren syndrome while anti-La was detected in serum of eight patients. The most independent predictors of Sjogren's syndrome in AITD patients are anti-La, ESR, and salivary gland sonographic change. Sjogren's syndrome has been found in patients with AITD, and also patients with AITD have symptoms that mimic sicca disease despite not fulfilling the criteria for diagnosis.","PeriodicalId":16112,"journal":{"name":"Journal of Investigative Medicine","volume":" ","pages":"271-278"},"PeriodicalIF":2.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142837184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Radiation therapy in combination with immune checkpoint inhibitors in metastatic lung cancer: Effect of fractionation. 快讯放疗联合免疫检查点抑制剂治疗转移性肺癌：分化的影响。

IF 2.5 4区医学

Journal of Investigative Medicine Pub Date : 2025-03-01 Epub Date: 2025-02-11 DOI: 10.1177/10815589241270439

Christopher Rosso, Melissa Reed, Natalie Walde, Ioannis A Voutsadakis

{"title":"Radiation therapy in combination with immune checkpoint inhibitors in metastatic lung cancer: Effect of fractionation.","authors":"Christopher Rosso, Melissa Reed, Natalie Walde, Ioannis A Voutsadakis","doi":"10.1177/10815589241270439","DOIUrl":"10.1177/10815589241270439","url":null,"abstract":"Immunotherapy with checkpoint inhibitors has improved the outcomes of patients with metastatic lung cancer in recent years. Despite improved prognosis, not all patients respond to treatment. Therapeutic interventions to build on the success of immune checkpoint inhibitors are needed. A retrospective review of patient records for patients who had received immune checkpoint inhibitors in a single cancer center over 4 years was undertaken. Demographic and disease characteristics of patients with metastatic non-small cell lung cancer were recorded. Data on other treatments including chemotherapy and radiation therapy were extracted, and survival outcomes were calculated. Most (81.8%) of the 77 metastatic lung cancer patients examined had received palliative radiation therapy within 3 months of starting immune checkpoint inhibitors. While the survival outcomes of these patients did not differ from patients who had not received radiotherapy, patients who had undergone hypofractionated radiotherapy (defined as one or more fractions of 700 cGy or higher) displayed a better overall survival (OS) than the rest of the cohort. Palliative radiation therapy administered in proximity with immune checkpoint inhibitors immunotherapy had no effect on the OS of metastatic lung cancer patients. However, patients receiving palliative radiotherapy with fractions above 700 cGy showed better OS. Further studies are needed to optimize a combination strategy.","PeriodicalId":16112,"journal":{"name":"Journal of Investigative Medicine","volume":" ","pages":"300-309"},"PeriodicalIF":2.5,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142621856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparing different viral strains in identifying risk factors for the development of venous thromboembolism in hospitalized COVID-19 patients.

IF 2.5 4区医学

Journal of Investigative Medicine Pub Date : 2025-02-20 DOI: 10.1177/10815589251320042

Harsimran Panesar, Ruchi Raval, Amber W Chan, Jana Tancredi, Gregory Simonian, David J O'Connor

{"title":"Comparing different viral strains in identifying risk factors for the development of venous thromboembolism in hospitalized COVID-19 patients.","authors":"Harsimran Panesar, Ruchi Raval, Amber W Chan, Jana Tancredi, Gregory Simonian, David J O'Connor","doi":"10.1177/10815589251320042","DOIUrl":"10.1177/10815589251320042","url":null,"abstract":"Since the onset of the coronavirus disease 2019 (COVID-19) pandemic, there have been nearly seven million deaths associated with COVID-19 and over thirteen billion total vaccine doses administered worldwide. This study aimed to evaluate the patient demographics and laboratory values that might help predict individuals at risk of having a deep vein thrombosis (DVT) formation while hospitalized with COVID-19. Focusing on the four major strains alongside the associated vaccination availability periods in the United States to devise risk stratification and management algorithms. This retrospective cohort study analyzed 4429 COVID-19-positive patients admitted to a single, tertiary care institution from March 2020 to February 2024 who underwent venous duplex ultrasound due to clinical suspicion. It was found that active chemotherapy treatment, higher weight (77.0 vs 84.0 kg), and longer LOS to first duplex (5 vs 10 days) were independent predictors of mortality. In addition, men, older age (68 vs 70 years old), medical history of DVTs, and longer LOS to first duplex (5 vs 8 days) were prognostic for DVT formation. More importantly, the prevalence of DVT was not statistically different between each COVID-19 wave, despite diminished mortality and morbidity over time. This study also demonstrated many non-predictive variables including COVID-19 as the principal diagnosis, BMI, smoking status, history of atrial fibrillation, stroke, CAD, vaccination status, and traditional laboratory values. Helping establish a critical foundation to analyze trends of anticoagulant and systemic corticosteroid use in this COVID-19 patient population.","PeriodicalId":16112,"journal":{"name":"Journal of Investigative Medicine","volume":" ","pages":"10815589251320042"},"PeriodicalIF":2.5,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143468310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0