Journal of Investigative Medicine最新文献

{"title":"EXPRESS: Protective Effects of Booster Dose of SARS-COV-2 Vaccination Against Post-Acute COVID-19 Syndrome: A Systematic Review.","authors":"Ved Patel, Maximilian Korsun, Joseph Steven Cervia","doi":"10.1177/10815589251346963","DOIUrl":"https://doi.org/10.1177/10815589251346963","url":null,"abstract":"<p><p>The global impact of COVID-19, caused by SARS-CoV-2, has extended beyond acute infection, with Post-Acute COVID-19 Syndrome (PACS) affecting an estimated 10% of recovered individuals. PACS manifests a range of debilitating symptoms, including fatigue, cognitive impairment, and gastrointestinal issues. While vaccination has proven effective in mitigating severe COVID-19 outcomes, the role of booster doses in preventing PACS remains unclear. This study aimed to evaluate whether COVID-19 booster vaccinations reduce the incidence and severity of PACS in individuals with prior SARS-CoV-2 infection. A systematic review and meta-analysis were conducted in adherence to PRISMA guidelines. Databases PubMed, Embase, and Cochrane were searched for peer-reviewed studies published in English from January 2020-August 2023. Inclusion criteria encompassed RCTs, prospective cohort studies, and case-control studies comparing PACS prevalence between booster recipients and non-recipients. Risk of bias was assessed using the Joanna Briggs Institute appraisal tool. Data synthesis included pooled prevalence estimates and narrative analyses. Of 849 identified studies, 22 met inclusion criteria, with 12 providing complete data for meta-analysis. Among 38,718 participants, a trend toward lower PACS prevalence was observed in booster recipients (RR: 0.66; 95%-CI: 0.41 - 1.09), though heterogeneity (I² = 98%) limited statistical significance. Risk of bias analysis classified most studies as low or moderate risk, with two high-risk studies reporting higher PACS rates in boosted individuals. This study suggests a potential protective effect of booster vaccinations against PACS, though findings were not statistically significant. Further research with larger, standardized cohorts is essential to validate these observations and guide vaccination strategies.</p>","PeriodicalId":16112,"journal":{"name":"Journal of Investigative Medicine","volume":" ","pages":"10815589251346963"},"PeriodicalIF":2.5,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144136093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EXPRESS: HMGA2-AS1 promotes the growth and cisplatin resistance in esophageal squamous cell carcinoma by enhancing HMGA2-dependent transcription of Snail2.","authors":"Yuping Yuan, Xiaolei Zhang, Hongli Liao, Wan-Chun Ye","doi":"10.1177/10815589251346948","DOIUrl":"https://doi.org/10.1177/10815589251346948","url":null,"abstract":"<p><p>Drug resistance restrains the efficacy of platinum-based chemotherapy against esophageal squamous cell carcinoma (ESCC). Long noncoding RNAs (lncRNAs) play crucial roles in various cancers. In the present study, we conducted bioinformatical analysis to search for key lncRNAs involved in ESCC progression. Experimental validations focused on a candidate lncRNA, HMGA2-AS1. The roles of the lncRNA HMGA2-AS1 in ESCC growth and cisplatin resistance were explored. The clinical significance of HMGA2-AS1 in ESCC was also determined. Our data demonstrated that HMGA2-AS1 was significantly upregulated in ESCC relative to adjacent normal tissues and correlated with tumor size (P = 0.0035) and tumor stage (P = 0.0001). Knockdown of HMGA2-AS1 suppressed the proliferation and colony formation and increased cisplatin sensitivity in ESCC cells. In vivo tumorigenic studies showed that depletion of HMGA2-AS1 impaired tumorigenesis of ESCC cells. Enforced expression of HMGA2-AS1 promoted ESCC proliferation and cisplatin resistance. HMGA2-AS1 interacted with HMGA2 protein in the nucleus of ESCC cells, promoting HMGA2-dependent transactivation of Snail2. Silencing of HMGA2 abrogated HMGA2-AS1-induced proliferation and cisplatin resistance in ESCC cells, which was rescued by overexpression of Snail2. In conclusion, our results highlight the importance of HMGA2-AS1 in ESCC growth and cisplatin resistance. HMGA2-AS1-induced aggressive phenotype in ESCC depends on the interaction with HMGA2. HMGA2-AS1 may serve as a potential therapeutic target for ESCC.</p>","PeriodicalId":16112,"journal":{"name":"Journal of Investigative Medicine","volume":" ","pages":"10815589251346948"},"PeriodicalIF":2.5,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144136091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EXPRESS: CircZFR involves propofol-triggered ferroptosis in lung cancer cells through the IGF2BP2/GPX4 axis.","authors":"Xiaocui Yu, Yuhui Gu, Shao Wang, Chunyang Yin, Xin Liao, Xucai Liao","doi":"10.1177/10815589251346947","DOIUrl":"https://doi.org/10.1177/10815589251346947","url":null,"abstract":"<p><p>This study aims to delineate the underlying mechanism by which propofol triggers ferroptosis in lung cancer cells through the inhibition of the circZFR/IGF2BP2/GPX4 axis. The expression levels of circZFR, IGF2BP2, and GPX4 in lung cancer cells were assessed using qPCR and Western blot analysis. Cell viability was evaluated with the CCK-8 assay, and ferroptosis-related indicators were measured using appropriate kits. The interactions between circZFR and IGF2BP2, as well as between GPX4 and IGF2BP2, were investigated through RNA pull-down and RNA immunoprecipitation (RIP) assays, and their effects on ferroptosis were analyzed using rescue assays. Additionally, xenograft assays in nude mice were conducted to evaluate the impact of propofol on tumor growth and ferroptosis in vivo. Propofol treatment induced cell ferroptosis, as evidenced decreased cell viability and elevated levels of MDA, Fe2+, and lipid ROS in H1299 and SPC-A-1 cells. Additionally, propofol reduced the expression of circZFR and GPX4 in lung cancer cells. Notably, the overexpression of circZFR inhibited propofol-induced ferroptosis in these cells. CircZFR interacts with IGF2BP2 to regulate the stability of GPX4 mRNA and its protein expression. Furthermore, circZFR inhibited GPX4-mediated ferroptosis by enhancing IGF2BP2 expression in both H1299 and SPC-A-1 cell lines. Moreover, propofol inhibited tumor growth in nude mice, downregulated the expression of circZFR, IGF2BP2, and GPX4, and increased MDA and Fe2+ levels in tumor tissues. Propofol downregulate circZFR to inhibit the expression of GPX4 by interacting with IGF2BP2, thereby triggering ferroptosis in lung cancer cells.</p>","PeriodicalId":16112,"journal":{"name":"Journal of Investigative Medicine","volume":" ","pages":"10815589251346947"},"PeriodicalIF":2.5,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144136160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EXPRESS: Algorithmic Screening of Advanced Liver Fibrosis in a High-Risk Population and Correlation with Transient Elastography Results.","authors":"Majd Helou, Mifleh Tatour, Fadi Abu Baker, Tarek Saadi, Ziv Neeman, Rawi Hazzan","doi":"10.1177/10815589251342189","DOIUrl":"https://doi.org/10.1177/10815589251342189","url":null,"abstract":"<p><p>Liver biopsy remains the diagnostic gold standard for assessing liver fibrosis severity; however, its cost and invasiveness highlight the need for accurate, non-invasive alternatives. This study evaluates the performance of FibroPredict, a novel algorithm, for detecting advanced liver fibrosis in high-risk populations. It compares its accuracy to transient elastography (TE), the current non-invasive reference standard. A retrospective cohort study of 316 high-risk individuals used electronic health record (EHR) data and routine laboratory results to calculate FibroPredict, APRI (Aspartate Aminotransferase-to-Platelet Ratio Index), and FIB-4 scores, which were then compared to liver stiffness measurements (LSM) obtained through TE. FibroPredict demonstrated a sensitivity of 96.8% and a negative predictive value (NPV) of 90.9% at a cutoff score of ≥135 for detecting advanced fibrosis (LSM ≥8 kPa), outperforming FIB-4 in sensitivity and the ability to rule out advanced fibrosis. APRI, at a cutoff of 1.5, showed high specificity (98.41%) but low sensitivity (6.32%), making it more suitable for confirming rather than excluding advanced fibrosis. However, FibroPredict's specificity was low (21.0%), resulting in higher false-positive rates. FIB-4, with a cutoff of ≥2.67, showed lower sensitivity but better specificity (46.8%). FibroPredict's excellent sensitivity and high NPV make it a promising tool for ruling out advanced fibrosis, particularly in resource-limited settings. However, its low specificity underscores the need for confirmatory tests, such as TE, or combining it with APRI to enhance diagnostic accuracy.</p>","PeriodicalId":16112,"journal":{"name":"Journal of Investigative Medicine","volume":" ","pages":"10815589251342189"},"PeriodicalIF":2.5,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144029090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Translational science and related disciplines\".","authors":"","doi":"10.1177/10815589251335165","DOIUrl":"https://doi.org/10.1177/10815589251335165","url":null,"abstract":"","PeriodicalId":16112,"journal":{"name":"Journal of Investigative Medicine","volume":" ","pages":"10815589251335165"},"PeriodicalIF":2.5,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144016883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction: \"Mir-3150B-3P Inhibits the Proliferation and Invasion of Cervical Cancer Cells by Targeting Tnfrsf11A\".","authors":"","doi":"10.1177/10815589251332430","DOIUrl":"https://doi.org/10.1177/10815589251332430","url":null,"abstract":"","PeriodicalId":16112,"journal":{"name":"Journal of Investigative Medicine","volume":" ","pages":"10815589251332430"},"PeriodicalIF":2.5,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144004243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Utility of serum NAMPT concentrations in clinical management of HPV-infected patients.","authors":"Alper Kahraman, Isın Ureyen, Guzin Aykal, Tayfun Toptas, Mustafa Gokkaya, Aysun Alcı, Necim Yalcin, Merve Cakir Kole, Selim Kandemir, Mehmet Goksu","doi":"10.1177/10815589251336745","DOIUrl":"https://doi.org/10.1177/10815589251336745","url":null,"abstract":"<p><p>The expression of nicotinamide-phosphoribosyl transferase (NAMPT) was demonstrated to increase in various dysplastic and malignant conditions, usually consistent with the severity of the disease. This study was conducted to assess the utility of extracellular NAMPT (eNAMPT) in the management of cervical dysplasia in human papillomavirus (HPV) infected women. Circulating eNAMPT concentrations in high-risk HPV-infected women who were diagnosed with high-grade squamous intraepithelial lesion (HSIL) or invasive cancer (cervical intraepithelial neoplasia 2+ (CIN2+) lesions) and who were revealed to have no cervical dysplasia or low-grade squamous intraepithelial lesion (LSIL) were evaluated and compared. One hundred fifty nine high-risk HPV-infected patients for cervical biopsies under colposcopy guidance between February 2022 and February 2023 were included in this case-control study. Study group composed of consecutively enrolled 84 women with histological diagnosis of HSIL or cervical cancer (CIN2+ lesions) and control group composed of consecutively enrolled 75 women with LSIL or normal cervical biopsies. Circulating eNAMPT concentrations of cases with CIN2+ lesions and cases with LSIL or normal cervical biopsies were compared. No significant difference was found between median peripheral venous blood eNAMPT concentration of cases with histologic diagnosis of CIN2+ lesions and cases with LSIL or normal cervical biopsies (9.4 ng/mL (0.19-192) vs 8.9 ng/mL (0.19-176.9); p = 0.07, respectively). Multivariate linear regression analysis revealed no independent predictor of circulating eNAMPT concentrations among possible predictor variables. In conclusion, circulating eNAMPT concentrations of cases with CIN2+ lesions and cases with LSIL or normal cervical biopsies were found to be similar. Further research that evaluates cervical fluid eNAMPT concentrations might define novel noninvasive tools in cervical dysplasia management.</p>","PeriodicalId":16112,"journal":{"name":"Journal of Investigative Medicine","volume":" ","pages":"10815589251336745"},"PeriodicalIF":2.5,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144024721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive analyses: Using machine learning models for mortality prediction in the intensive care unit of internal medicine.","authors":"Ahmed Cihad Genç, Ensar Özmen, Deniz Çekiç, Kubilay İşsever, Fevziye Türkoğlu Genç, Ahmed Bilal Genç, Aysel Toçoğlu, Yusuf Durmaz, Hüseyin Özkök, Selçuk Yaylacı","doi":"10.1177/10815589251335327","DOIUrl":"https://doi.org/10.1177/10815589251335327","url":null,"abstract":"<p><p>Mortality prediction in the intensive care unit (ICU) is essential in patient management. Emerging methods such as machine learning (ML) can be employed to predict ICU patients' mortality. Patients receiving treatment in the ICU of the internal medicine department were subjected to ML analysis upon admission, considering demographic, laboratory, and medical scores. Data from 787 internal medicine ICU patients were analyzed, with only a subset (220) included in the study for the 30-day mortality prediction model. The performance of boosting and Logistic Regression models in mortality prediction was compared. Categorical boosting (CatBoost) achieved the highest area under the curve (AUC) of 0.90, while extreme gradient boosting reached a maximum AUC of 0.85, and Logistic Regression attained the highest AUC of 0.83. Incorporating Acute Physiology and Chronic Health Evaluation II, Simplified Acute Physiology Score II, and Sequential Organ Failure Assessment scores with clinical and laboratory values, CatBoost demonstrated the strongest predictive performance with high sensitivity and specificity. In the ICU of the internal medicine department, it was concluded that the ML models successfully predict mortality.</p>","PeriodicalId":16112,"journal":{"name":"Journal of Investigative Medicine","volume":" ","pages":"10815589251335327"},"PeriodicalIF":2.5,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144013159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EXPRESS: Spexin as a Potential Biomarker for Autoimmune Inflammation in Graves' Disease.","authors":"Betul Cigdem Yortanli, Ummugulsum Can, Mehmet Yortanlı, Korhan Kollu, Selma Ozlem Celikdelen, Gulsum Tugce Catak, Oguzhan Aksu, Muhammet Kızılarslanoglu","doi":"10.1177/10815589251335050","DOIUrl":"https://doi.org/10.1177/10815589251335050","url":null,"abstract":"<p><p>Graves' disease (GD) is an autoimmune thyroid disorder characterized by excessive thyroid hormone production driven by thyroid-stimulating hormone receptor antibodies (TRAb). This study aimed to investigate the relationship between serum spexin (SPX) levels, TRAb levels, thyroid ultrasound findings, and metabolic parameters in GD patients, while evaluating SPX as a potential biomarker for autoimmune inflammation. A prospective, single-center study included 45 GD patients and 45 healthy controls. Serum TSH, fT3, fT4, anti-thyroglobulin, anti-thyroid peroxidase, TRAb, and SPX levels were measured. Thyroid ultrasonography classified patients into mild, moderate, and high heterogeneity groups. SPX levels were significantly higher in GD patients compared to controls (p < .001) and showed a strong positive correlation with TRAb levels (r = .579, p < .001) and thyroid heterogeneity (p < .001). Newly diagnosed patients (<6 months) exhibited the highest SPX levels, which decreased with prolonged disease duration. Receiver Operating Characteristic (ROC) analysis identified a cutoff value of 105 pg/mL for SPX, yielding 60% sensitivity and 91.1% specificity (AUC: 0.765, p < .001). SPX levels were also inversely correlated with disease duration and positively associated with inflammatory markers, suggesting its utility in monitoring disease activity and progression. These findings highlight SPX as a novel biomarker for assessing disease severity and autoimmune inflammation in GD. Incorporating SPX measurements into clinical practice may aid in early diagnosis, disease stratification, and therapeutic monitoring, ultimately improving personalized care for GD patients.</p>","PeriodicalId":16112,"journal":{"name":"Journal of Investigative Medicine","volume":" ","pages":"10815589251335050"},"PeriodicalIF":2.5,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143780367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pediatric AML prognostication with an m6A-focused LASSO model.","authors":"Jing Zhang, Taoli Suo, Yanli Cheng, Hongyu Shi, Haiyan Lu","doi":"10.1177/10815589251334964","DOIUrl":"10.1177/10815589251334964","url":null,"abstract":"<p><p>Acute myeloid leukemia (AML) presents varying prognoses in pediatric and adult patients, with dysregulated N6-methyladenosine (m6A)-related genes playing a pivotal role in its pathogenesis. Through an analysis of single-cell RNA sequencing data from the Gene Expression Omnibus, we uncovered distinct patterns of m6A gene expression specific to pediatric AML (pAML) cases. Additionally, we devised a LASSO risk regression model to evaluate prognosis within different AML subsets, showcasing the clinical utility of m6A-related genes in predicting AML outcomes. Our study underscores the unique and significant m6A gene expression profiles observed in pAML, emphasizing their potential relevance in prognostic assessment. The demonstrated effectiveness of the LASSO risk regression model further underscores the importance of m6A-related genes as valuable predictors of AML outcomes. These findings not only unveil novel insights into the prognostic implications of m6A-related genes in pAML but also advocate for their application as prognostic markers to enhance personalized treatment strategies in both pAML and adult AML populations.</p>","PeriodicalId":16112,"journal":{"name":"Journal of Investigative Medicine","volume":" ","pages":"10815589251334964"},"PeriodicalIF":2.5,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143780355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}