Journal of Investigative Medicine最新文献

Utility of serum NAMPT concentrations in clinical management of HPV-infected patients. 血清NAMPT浓度在hpv感染患者临床管理中的应用。

IF 2.5 4区医学

Journal of Investigative Medicine Pub Date : 2025-08-01 Epub Date: 2025-04-12 DOI: 10.1177/10815589251336745

Alper Kahraman, Isın Ureyen, Guzin Aykal, Tayfun Toptas, Mustafa Gokkaya, Aysun Alcı, Necim Yalcin, Merve Cakir Kole, Selim Kandemir, Mehmet Goksu

{"title":"Utility of serum NAMPT concentrations in clinical management of HPV-infected patients.","authors":"Alper Kahraman, Isın Ureyen, Guzin Aykal, Tayfun Toptas, Mustafa Gokkaya, Aysun Alcı, Necim Yalcin, Merve Cakir Kole, Selim Kandemir, Mehmet Goksu","doi":"10.1177/10815589251336745","DOIUrl":"10.1177/10815589251336745","url":null,"abstract":"The expression of nicotinamide-phosphoribosyl transferase (NAMPT) was demonstrated to increase in various dysplastic and malignant conditions, usually consistent with the severity of the disease. This study was conducted to assess the utility of extracellular NAMPT (eNAMPT) in the management of cervical dysplasia in human papillomavirus (HPV) infected women. Circulating eNAMPT concentrations in high-risk HPV-infected women who were diagnosed with high-grade squamous intraepithelial lesion (HSIL) or invasive cancer (cervical intraepithelial neoplasia 2+ (CIN2+) lesions) and who were revealed to have no cervical dysplasia or low-grade squamous intraepithelial lesion (LSIL) were evaluated and compared. One hundred fifty nine high-risk HPV-infected patients for cervical biopsies under colposcopy guidance between February 2022 and February 2023 were included in this case-control study. Study group composed of consecutively enrolled 84 women with histological diagnosis of HSIL or cervical cancer (CIN2+ lesions) and control group composed of consecutively enrolled 75 women with LSIL or normal cervical biopsies. Circulating eNAMPT concentrations of cases with CIN2+ lesions and cases with LSIL or normal cervical biopsies were compared. No significant difference was found between median peripheral venous blood eNAMPT concentration of cases with histologic diagnosis of CIN2+ lesions and cases with LSIL or normal cervical biopsies (9.4 ng/mL (0.19-192) vs 8.9 ng/mL (0.19-176.9); p = 0.07, respectively). Multivariate linear regression analysis revealed no independent predictor of circulating eNAMPT concentrations among possible predictor variables. In conclusion, circulating eNAMPT concentrations of cases with CIN2+ lesions and cases with LSIL or normal cervical biopsies were found to be similar. Further research that evaluates cervical fluid eNAMPT concentrations might define novel noninvasive tools in cervical dysplasia management.","PeriodicalId":16112,"journal":{"name":"Journal of Investigative Medicine","volume":" ","pages":"514-520"},"PeriodicalIF":2.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144024721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cross-sectional analysis of disability education in American Medical Schools. 美国医学院残疾教育的横断面分析。

IF 2.5 4区医学

Journal of Investigative Medicine Pub Date : 2025-08-01 Epub Date: 2025-04-02 DOI: 10.1177/10815589251334961

Lauren Garrison, Tanner Mooney, Kendall Lucara, Sebastien Fuchs, Johannie M Spaan, Belinda McCully

{"title":"Cross-sectional analysis of disability education in American Medical Schools.","authors":"Lauren Garrison, Tanner Mooney, Kendall Lucara, Sebastien Fuchs, Johannie M Spaan, Belinda McCully","doi":"10.1177/10815589251334961","DOIUrl":"10.1177/10815589251334961","url":null,"abstract":"Greater than 25% of the American population has a disability. The demand for disability-informed physicians underscores the need to better prepare physicians to care for patients with disabilities. This study presents findings from three Qualtrics survey studies that investigated (1) medical students' exposure to patients with disabilities while on clinical rotations at Western University of Health Sciences, (2) students' perceived preparedness to care for patients with disabilities nationwide, and (3) the effectiveness of Special Olympics online modules to teach students about caring for patients with disabilities. Descriptive analysis from these studies together indicated that medical students did not feel prepared to care for patients with disabilities. To remedy this, Special Olympics online modules for healthcare professionals were found to effectively enhance student physician training. These findings highlight a critical need for improved medical training to prepare the next generation of physicians to care for patients with disabilities, while outlining a possible educational tool that could be incorporated into medical school curriculum.","PeriodicalId":16112,"journal":{"name":"Journal of Investigative Medicine","volume":" ","pages":"471-480"},"PeriodicalIF":2.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143772424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unlocking the immune puzzle: T Cell exhaustion in cirrhosis and implication for immunotherapy. EXPRESS：解开免疫之谜：肝硬化中的T细胞衰竭及其对免疫治疗的影响。

IF 2.5 4区医学

Journal of Investigative Medicine Pub Date : 2025-08-01 Epub Date: 2025-01-31 DOI: 10.1177/10815589251320368

Geeta Yadav, Amit Goel, Manish Kumar, Hardeep Singh Malhotra, Harshita Katiyar, Himanshu Dandu

{"title":"Unlocking the immune puzzle: T Cell exhaustion in cirrhosis and implication for immunotherapy.","authors":"Geeta Yadav, Amit Goel, Manish Kumar, Hardeep Singh Malhotra, Harshita Katiyar, Himanshu Dandu","doi":"10.1177/10815589251320368","DOIUrl":"10.1177/10815589251320368","url":null,"abstract":"Cirrhosis, an advanced stage of liver disease, induces cirrhosis-associated immune dysfunction syndrome (CAIDS), characterized by both innate and adaptive immune dysfunction. Inflammation triggered by factors such as alcohol, viruses, toxins, and cholesterol induces metabolic reprogramming of both innate and adaptive immune cells. Our study specifically sought to investigate the compromised adaptive immune response in cirrhosis by focusing on assessing T-cell exhaustion and activation markers on helper and cytotoxic T cells. A prospective observational study involving 19 liver cirrhosis patients and 36 healthy controls was conducted. The hepatic decompensation degree was assessed using various parameters, including serum bilirubin, albumin, international normalized ratio, ascites, and hepatic encephalopathy. T cell activation (CD38, CD44, CD69, HLADR) and exhaustion markers (CTLA-4, PD-1, TIM-3, LAG-3) were assessed on helper and cytotoxic T cells by flow cytometry. Cirrhosis patients showed reduced T cells with no alteration in the CD4:CD8 T cell ratio. Among activation markers, HLADR showed increased expression on CD8+ T cells (p = 0.031). Regarding exhaustion markers, LAG-3 and TIM-3 exhibited increased expression in cirrhotic patients compared to controls in both CD4 and CD8 T cells (p = 0.004, p = 0.016, p = 0.001, p = 0.004, respectively). Neither cirrhotic nor healthy controls showed CTLA expression. PD-1 did not differ significantly between the two groups. Co-expression of PD-1/TIM-3 on CD8+ T cells was notably higher in cirrhotic patients (p < 0.002). The observation of impaired adaptive immunity with notable T-cell exhaustion and activation in cirrhosis underscores the potential relevance of immunotherapy.","PeriodicalId":16112,"journal":{"name":"Journal of Investigative Medicine","volume":" ","pages":"481-489"},"PeriodicalIF":2.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143074595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive analyses: Using machine learning models for mortality prediction in the intensive care unit of internal medicine. 综合分析：利用机器学习模型预测内科重症监护病房的死亡率。

IF 2.5 4区医学

Journal of Investigative Medicine Pub Date : 2025-08-01 Epub Date: 2025-04-09 DOI: 10.1177/10815589251335327

Ahmed Cihad Genç, Ensar Özmen, Deniz Çekiç, Kubilay İşsever, Fevziye Türkoğlu Genç, Ahmed Bilal Genç, Aysel Toçoğlu, Yusuf Durmaz, Hüseyin Özkök, Selçuk Yaylacı

{"title":"Comprehensive analyses: Using machine learning models for mortality prediction in the intensive care unit of internal medicine.","authors":"Ahmed Cihad Genç, Ensar Özmen, Deniz Çekiç, Kubilay İşsever, Fevziye Türkoğlu Genç, Ahmed Bilal Genç, Aysel Toçoğlu, Yusuf Durmaz, Hüseyin Özkök, Selçuk Yaylacı","doi":"10.1177/10815589251335327","DOIUrl":"10.1177/10815589251335327","url":null,"abstract":"Mortality prediction in the intensive care unit (ICU) is essential in patient management. Emerging methods such as machine learning (ML) can be employed to predict ICU patients' mortality. Patients receiving treatment in the ICU of the internal medicine department were subjected to ML analysis upon admission, considering demographic, laboratory, and medical scores. Data from 787 internal medicine ICU patients were analyzed, with only a subset (220) included in the study for the 30-day mortality prediction model. The performance of boosting and Logistic Regression models in mortality prediction was compared. Categorical boosting (CatBoost) achieved the highest area under the curve (AUC) of 0.90, while extreme gradient boosting reached a maximum AUC of 0.85, and Logistic Regression attained the highest AUC of 0.83. Incorporating Acute Physiology and Chronic Health Evaluation II, Simplified Acute Physiology Score II, and Sequential Organ Failure Assessment scores with clinical and laboratory values, CatBoost demonstrated the strongest predictive performance with high sensitivity and specificity. In the ICU of the internal medicine department, it was concluded that the ML models successfully predict mortality.","PeriodicalId":16112,"journal":{"name":"Journal of Investigative Medicine","volume":" ","pages":"460-470"},"PeriodicalIF":2.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144013159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serum Talin-1 and Pentraxin-3 levels as biomarkers for vulnerable plaques in STEMI: Correlation with SYNTAX and Gensini scores. EXPRESS：血清Talin-1和pentaxin -3水平作为STEMI易损斑块的生物标志物：与句法和Gensini评分相关。

IF 2.5 4区医学

Journal of Investigative Medicine Pub Date : 2025-08-01 Epub Date: 2025-04-02 DOI: 10.1177/10815589251333444

Rukiye Nar, Harun Akarsu, Gokay Nar, Sara Cetin Sanlialp, Gursel Sen, Isik Tekin

{"title":"Serum Talin-1 and Pentraxin-3 levels as biomarkers for vulnerable plaques in STEMI: Correlation with SYNTAX and Gensini scores.","authors":"Rukiye Nar, Harun Akarsu, Gokay Nar, Sara Cetin Sanlialp, Gursel Sen, Isik Tekin","doi":"10.1177/10815589251333444","DOIUrl":"10.1177/10815589251333444","url":null,"abstract":"Cardiovascular diseases, especially ischemic heart diseases, are the leading cause of death globally, highlighting the need for improved diagnostic tools for vulnerable plaques. This study aimed to evaluate the potential of soluble Talin-1 (sTalin-1) and Pentraxin-3 (PTX-3) as biomarkers in patients with ST-elevation myocardial infarction (STEMI). Conducted as a single-center observational study, it included 40 STEMI patients and 30 controls with normal coronary arteries. Serum levels of sTalin-1 and PTX-3 were measured using enzyme-linked immunosorbent assays (ELISA), and disease severity was assessed with SYNTAX and Gensini scores. Results showed that STEMI patients had significantly lower levels of sTalin-1 and higher levels of PTX-3 compared to controls. ROC curve analysis revealed an area under the curve (AUC) of 0.785 for sTalin-1 and 0.702 for PTX-3 in differentiating between STEMI patients and controls. While sTalin-1 demonstrated high specificity but low sensitivity, PTX-3 exhibited moderate sensitivity and specificity. Talin-1 levels negatively correlated with SYNTAX and Gensini scores, suggesting its role in assessing disease severity. These findings suggest that decreased serum levels of sTalin-1 and increased levels of PTX-3 in STEMI patients could serve as potential biomarkers for identifying vulnerable plaques and assessing disease severity. The study supports the potential use of sTalin-1 and PTX-3 in the diagnostic and prognostic evaluation of acute coronary syndromes.","PeriodicalId":16112,"journal":{"name":"Journal of Investigative Medicine","volume":" ","pages":"504-513"},"PeriodicalIF":2.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143764097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Challenging biological samples and strategies for DNA extraction. EXPRESS：挑战生物样品和DNA提取策略。

IF 2.5 4区医学

Journal of Investigative Medicine Pub Date : 2025-08-01 Epub Date: 2025-03-03 DOI: 10.1177/10815589251327503

Ajay K Rana

{"title":"Challenging biological samples and strategies for DNA extraction.","authors":"Ajay K Rana","doi":"10.1177/10815589251327503","DOIUrl":"10.1177/10815589251327503","url":null,"abstract":"Despite the significant progress of DNA profiling in forensic science, the extraction of DNA from challenging samples remains a formidable obstacle in forensic laboratories. Particularly, hard tissues (bones, teeth, hair, and nails), formalin-fixed tissues, and contaminated samples pose considerable difficulties. DNA extraction from such samples is often complicated, resulting in scanty, degraded, and contaminated DNA. Moreover, the presence of inhibitors from the surrounding environment further hinders DNA quantification and amplification, presenting additional challenges. This review article delves into the molecular basis of these challenges and examines the mechanistic principles underlying standard DNA extraction protocols. To overcome these obstacles, skilled efforts and additional pre-processing techniques are generally required before organic or silica column-based DNA extraction. Such techniques may involve scraping the waste, sample cleaning with detergents, disinfecting, demineralization of bones and teeth, long proteolytic enzyme treatment, in some cases, harsh methods like hot alkali treatment, tailored to the specific sample type. By addressing these challenges and understanding the molecular intricacies involved in DNA extraction, forensic scientists can improve the reliability and success rate of DNA analysis from difficult forensic samples. This review serves as a comprehensive resource for understanding the complexities of DNA extraction and offers potential solutions for recovering the DNA from highly challenging samples.","PeriodicalId":16112,"journal":{"name":"Journal of Investigative Medicine","volume":" ","pages":"443-459"},"PeriodicalIF":2.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pediatric AML prognostication with an m6A-focused LASSO model. EXPRESS：以m6a为中心的Lasso模型预测儿童AML

IF 2.5 4区医学

Journal of Investigative Medicine Pub Date : 2025-08-01 Epub Date: 2025-04-04 DOI: 10.1177/10815589251334964

Jing Zhang, Taoli Suo, Yanli Cheng, Hongyu Shi, Haiyan Lu

引用次数: 0

Retraction: "Mir-3150B-3P Inhibits the Proliferation and Invasion of Cervical Cancer Cells by Targeting Tnfrsf11A". 撤稿：Mir-3150B-3P靶向Tnfrsf11A抑制宫颈癌细胞增殖和侵袭

IF 2.5 4区医学

Journal of Investigative Medicine Pub Date : 2025-06-01 Epub Date: 2025-04-14 DOI: 10.1177/10815589251332430

引用次数: 0

Corrigendum to "GDF11 Level and Its Effect on Prognosis in Patients with Acute Myeloid Leukemia". “GDF11水平及其对急性髓性白血病患者预后的影响”的更正。

IF 2.5 4区医学

Journal of Investigative Medicine Pub Date : 2025-06-01 Epub Date: 2025-04-01 DOI: 10.1177/10815589251330477

引用次数: 0

Response to: Faculty should be cautiously optimistic about the role of generative AI in medical education. 对以下问题的回应：教师应该对生成式人工智能在医学教育中的作用持谨慎乐观的态度。

IF 2.5 4区医学

Journal of Investigative Medicine Pub Date : 2025-06-01 Epub Date: 2025-03-28 DOI: 10.1177/10815589251333745

Jorge L Cervantes

引用次数: 0