Journal of Food and Nutrition Research最新文献_第10页

Isolation and Cloning of sprA Gene Alkaline Protease Activity from Streptomyces flavogriseus ADEM7 and Characterization of Its Antioxidant and Antitumor Activities 黄灰链霉菌ADEM7中sprA基因碱性蛋白酶活性的分离、克隆及其抗氧化和抗肿瘤活性的研究

IF 1.1 4区农林科学

Journal of Food and Nutrition Research Pub Date : 2023-05-09 DOI: 10.12691/jfnr-11-5-2

Doaa E. El-Hadedy, Nesreen A. Safwat, Abir Pertila, Rafat A. Siddiqui

{"title":"Isolation and Cloning of sprA Gene Alkaline Protease Activity from Streptomyces flavogriseus ADEM7 and Characterization of Its Antioxidant and Antitumor Activities","authors":"Doaa E. El-Hadedy, Nesreen A. Safwat, Abir Pertila, Rafat A. Siddiqui","doi":"10.12691/jfnr-11-5-2","DOIUrl":"https://doi.org/10.12691/jfnr-11-5-2","url":null,"abstract":"In the food industry, alkaline protease plays an important role in the formation of value-added products. Alkaline protease also plays a significant role in waste management. In this study, to construct a stable and high-yield alkaline protease producer, we investigated a combined strategy of gamma radiation as an enhancement factor and alkaline protease PGEM-T easy construct . The present study was focused on the isolation of the sprA gene-producing alkaline protease from Streptomyces flavogriseus ADEM7 (AB723783.1). Optimum growth media, fermentation conditions, and proteolytic activity assay were evaluated. The result showed that the free cells preparation produced alkaline protease with a specific activity of 550 U/mg after being exposed to gamma radiation at 10 KGy. The sprA gene was detected using polymerase chain reaction followed by cloning in PGEM-T easy vector, transformation in E. coli JM109, and direct construct sequencing. The s prA gene encoding streptogrisin a (alkaline protease-like activity) was isolated in this study and recorded in the gene bank with accession number AB827411.1. The extracellular protease enzyme was tested for antioxidant and anticancer activities. In future work, we will extract this alkaline protease from the same strain on a larger scale using a fermenter and will characterize its antioxidant activity under in vivo conditions.","PeriodicalId":16096,"journal":{"name":"Journal of Food and Nutrition Research","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47861437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Physicochemical, Mechanical, and Structural Properties of the Femur from Rats Fed with Corn Tortillas (Zea mays L.) Prepared with Calcium Sulfate 硫酸钙制备玉米托（Zea mays L.）饲养大鼠股骨的理化、力学和结构特性

IF 1.1 4区农林科学

Journal of Food and Nutrition Research Pub Date : 2023-04-27 DOI: 10.12691/jfnr-11-4-7

Ma. Guadalupe Garnica-Romo, Diana Cecilia Maya-Cortés, Juan de Dios Figueroa Cárdenas, José Juan Véles-Medina, Rafael Zamora-Vega, Héctor Eduardo Martínez-Flores

引用次数: 0

Effects on Glyco-Metabolic Control after 12 Months of Treatment with a Supplement of Ilex Paraguariensis, White Mulberry and Chromium Picolinate in Non-Diabetic Patients with Dysglycemia 补充木麻黄、白桑树和吡啶甲酸铬治疗非糖尿病合并血糖异常患者12个月后对糖代谢控制的影响

IF 1.1 4区农林科学

Journal of Food and Nutrition Research Pub Date : 2023-04-27 DOI: 10.12691/jfnr-11-4-6

G. Derosa, A. D'Angelo, P. Maffioli

{"title":"Effects on Glyco-Metabolic Control after 12 Months of Treatment with a Supplement of Ilex Paraguariensis, White Mulberry and Chromium Picolinate in Non-Diabetic Patients with Dysglycemia","authors":"G. Derosa, A. D'Angelo, P. Maffioli","doi":"10.12691/jfnr-11-4-6","DOIUrl":"https://doi.org/10.12691/jfnr-11-4-6","url":null,"abstract":"The primary endpoint of the current study was to evaluate whether the effects previously demonstrated in the 3-month study could be maintained for 12 months. Patients were randomized to take Glicoset® in addition to a standardized diet, or to follow a standardized diet alone for 12 months. Glicoset® was self-administered once a day, 1 tablet during the breakfast. After 12 months, 31.9 % of patients retuned to a normal glycemic status in the group treated with nutraceutical + diet vs 3.3 % of patients in diet alone group (p < 0.05). At the end of the study 43.6% were classified as IFG in the group treated with nutraceutical + diet group vs 37% in diet alone group (p < 0.05). In the group treated with nutraceutical + diet, 24.5% were classified as IGT at the end of the study vs 51.1% in diet alone group (p < 0.01). In diet alone group, 8.7% developed type 2 diabetes mellitus vs 0 patients in diet + nutraceutical group (p < 0.05). Total cholesterol, LDL-C, and Tg were lower in the group treated with the nutraceutical combination. The positive effects on glycemic and lipid profile already showed in a 3 months study were also maintained after 12 months of treatment with a nutraceutical containing Ilex Paraguariensis, White Mulberry and Chromium Picolinate.","PeriodicalId":16096,"journal":{"name":"Journal of Food and Nutrition Research","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43780068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-wide Association Functional Studies (GWAFS) of Candidate Genes for Germination-related Traits of Low Temperature Tolerance in Maize (Zea mays L.) 玉米耐低温发芽相关性状候选基因的全基因组关联功能研究

IF 1.1 4区农林科学

Journal of Food and Nutrition Research Pub Date : 2023-04-21 DOI: 10.12691/jfnr-11-4-5

Lin Heying, Ren Zhiqiang, H. Noor

引用次数: 0

Effect of n-Hexane Extract of Camellia Oleifera Fruit Hull on Inflammatory Responses in LPS-Induced RAW264.7 Cells 油茶果壳正己烷提取物对LPS诱导的RAW264.7细胞炎症反应的影响

IF 1.1 4区农林科学

Journal of Food and Nutrition Research Pub Date : 2023-04-05 DOI: 10.12691/jfnr-11-4-4

引用次数: 0

Analysis of Genetic Diversity and Construction Fingerprint of Protein Content of Quinoa (Chenopodium quinoa Willd.) Vegetable Leaf 藜麦蔬菜叶片蛋白质含量的遗传多样性分析及构建指纹图谱

IF 1.1 4区农林科学

Journal of Food and Nutrition Research Pub Date : 2023-04-05 DOI: 10.12691/jfnr-11-4-3

引用次数: 0

Evolution of Psychrotrophic Bacteria on Beef During Refrigeration 牛肉冷藏过程中嗜冷菌的进化

IF 1.1 4区农林科学

Journal of Food and Nutrition Research Pub Date : 2023-03-30 DOI: 10.12691/jfnr-11-4-2

Benaissa Atika, Babelhadj Baaissa, Mimouni Yamina, Touhami Imene

引用次数: 0

Are Nutrition Blogs a Trustworthy Source? Author Education, Evidence-Based Writing, and Types of Diets 营养博客是值得信赖的来源吗？作者教育、循证写作与饮食类型

IF 1.1 4区农林科学

Journal of Food and Nutrition Research Pub Date : 2023-03-28 DOI: 10.12691/jfnr-11-4-1

Erfan Khazaee, Jeanne H. Freeland-Graves

引用次数: 0

Sulforaphane (SFN) Exerts Anti-inflammatory Effects on LPS-stimulated RAW 264.7 and Mouse Bone Marrow Driven Macrophages by Modulating p65 Phosphorylation and Histone Acetylation 硫福拉芬（SFN）通过调节p65磷酸化和组蛋白乙酰化对LPS刺激的RAW 264.7和小鼠骨髓驱动巨噬细胞的抗炎作用

IF 1.1 4区农林科学

Journal of Food and Nutrition Research Pub Date : 2023-03-27 DOI: 10.12691/jfnr-11-3-12

Seok-Yeong Yu, Zhenhua Liu, S. Chung, Young‐Cheul Kim

引用次数: 0

Analysis of the Nutritional Quality of Cookies with Various Food Commodities in Management of Malnutrition 营养不良管理中不同食品饼干的营养品质分析

IF 1.1 4区农林科学

Journal of Food and Nutrition Research Pub Date : 2023-03-24 DOI: 10.12691/jfnr-11-3-11

Rumida C, Tiar Lince Bakara, Ginta Siahaan

引用次数: 0