Journal of immunoassay最新文献_第8页

Modification of T-cell antigenic properties of tetanus toxoid by SDS-PAGE separation. Implications for T-cell blotting. SDS-PAGE分离修饰破伤风类毒素t细胞抗原性。对t细胞印迹的影响。

Journal of immunoassay Pub Date : 1997-05-01 DOI: 10.1080/01971529708005809

C B Christensen, T G Theander

引用次数: 0

Indirect enzyme-linked method for determining biotin in human serum. 间接酶联法测定人血清中生物素。

Journal of immunoassay Pub Date : 1997-02-01 DOI: 10.1080/01971529708005802

J O Nyalala, E Livaniou, L Leondiadis, G P Evangelatos, D S Ithakissios

{"title":"Indirect enzyme-linked method for determining biotin in human serum.","authors":"J O Nyalala, E Livaniou, L Leondiadis, G P Evangelatos, D S Ithakissios","doi":"10.1080/01971529708005802","DOIUrl":"https://doi.org/10.1080/01971529708005802","url":null,"abstract":"An indirect enzyme-linked assay was developed for quantifying biotin concentrations in human sera. Biotin standard solutions or unknown samples are preincubated with streptavidin-conjugated horseradish peroxidase (streptavidin-HRP) and added to plates coated with biotinylated bovine IgG (B-IgGb). The concentration of the streptavidin-HRP is such that the streptavidin binding sites are sufficient to bind apparently all the biotin present in samples, whereas, the remaining sites are inversely proportional to the amount of biotin in analysed sample. These sites could subsequently interact with the immobilized B-IgGb providing signal. The assay demonstrated dynamic range 5 to 640 ng/L, detection limit 2 ng/L, intra- and interassay C.V., 1.6-3.9% and 3.7-7.2% respectively, recovery 100-114% and linear recovery 90-117%. Serum biotin determined: healthy individuals 66 to 600 ng/L, pregnant women (> or = 36 weeks) 60 to 360 ng/L, and patients under chronic haemodialysis 0.56 to 1.62 micrograms/L. The method described is among those few which have been experimentally evaluated for their capabilitity of assessing biotin in human sera.","PeriodicalId":16060,"journal":{"name":"Journal of immunoassay","volume":"18 1","pages":"1-19"},"PeriodicalIF":0.0,"publicationDate":"1997-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01971529708005802","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20087304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Interaction between antibodies and hapten-protein conjugates of different composition: theoretical predictions and experimental data. 抗体和不同组成的半抗原蛋白偶联物之间的相互作用:理论预测和实验数据。

Journal of immunoassay Pub Date : 1997-02-01 DOI: 10.1080/01971529708005805

A V Zherdev, O G Romanenko, B B Dzantiev

引用次数: 6

Microparticle-enhanced nephelometric immunoassay of alpha-lactalbumin in human milk. 人乳中α -乳清蛋白的微粒增强浊度法免疫测定。

Journal of immunoassay Pub Date : 1997-02-01 DOI: 10.1080/01971529708005806

M L Cuillière, M Abbadi, C Molé, P Montagne, M C Béné, G Faure

{"title":"Microparticle-enhanced nephelometric immunoassay of alpha-lactalbumin in human milk.","authors":"M L Cuillière, M Abbadi, C Molé, P Montagne, M C Béné, G Faure","doi":"10.1080/01971529708005806","DOIUrl":"https://doi.org/10.1080/01971529708005806","url":null,"abstract":"A microparticle-enhanced nephelometric immunoassay was developed for alpha-lactalbumin quantitation in human milk. It is based on the nephelometric measurement of the light scattered during the competitive immunoagglutination of a microparticle-alpha-lactalbumin conjugate with an anti-alpha-lactalbumin antiserum. This immunoassay is sensitive (detection limit in reaction mixture, 1.5 micrograms/L) and could be performed in high dilution of milk, excluding any interference or sample pretreatment. It allowed the quantification of alpha-lactalbumin on a large range of concentrations (0.5-16.9 g/L) with accuracy (linear recovery in dilution-overloading assay) and precision (within- and between-run coefficients of variation from 1 to 7%). Changes in the alpha-lactalbumin concentration of human milk during lactation were determined in 162 samples. The concentration and ratio of alpha-lactalbumin total protein were found to be significantly lower in colostrum (4.9 g/l, 27%) than in transitional milk (5.2 g/L, 40%), then decreased in mature milk (3.4 g/L, 31%).","PeriodicalId":16060,"journal":{"name":"Journal of immunoassay","volume":"18 1","pages":"97-109"},"PeriodicalIF":0.0,"publicationDate":"1997-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01971529708005806","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20086518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

A descriptive model for the kinetics of a homogeneous fluorometric immunoassay. 均匀荧光免疫测定动力学的描述性模型。

Journal of immunoassay Pub Date : 1997-02-01 DOI: 10.1080/01971529708005803

E Zuber, L Rosso, B Darbouret, F Socquet, G Mathis, J P Flandrois

引用次数: 28

A procedure for the detection of free thiol-containing proteins on a polyvinylidene difluoride membrane. 用于检测聚偏二氟乙烯膜上的游离含硫醇蛋白质的程序。

Journal of immunoassay Pub Date : 1997-02-01 DOI: 10.1080/01971529708005807

T Kamiya

引用次数: 1

An immunoligand assay for quantitation of process specific Escherichia coli host cell contaminant proteins in a recombinant bovine somatotropin. 重组牛生长激素中工艺特异性大肠杆菌宿主细胞污染物蛋白的免疫配位定量分析。

Journal of immunoassay Pub Date : 1997-02-01 DOI: 10.1080/01971529708005804

M L Whitmire, L C Eaton