Journal of immunoassay最新文献_第7页

Characterization of monoclonal antibody reactive with 10-hydroxy-12(Z)-octadecenoic acid (10-OHODA) and its demonstration in cultured human macrophages. 与10-羟基-12(Z)-十八烯酸(10-OHODA)反应的单克隆抗体的鉴定及其在培养的人巨噬细胞中的证明。

Journal of immunoassay Pub Date : 1997-11-01 DOI: 10.1080/01971529708005824

Y Yamada, M Nagao, T Takatori, H Iwase, M Nakajima, K Iwadate

{"title":"Characterization of monoclonal antibody reactive with 10-hydroxy-12(Z)-octadecenoic acid (10-OHODA) and its demonstration in cultured human macrophages.","authors":"Y Yamada, M Nagao, T Takatori, H Iwase, M Nakajima, K Iwadate","doi":"10.1080/01971529708005824","DOIUrl":"https://doi.org/10.1080/01971529708005824","url":null,"abstract":"10-Hydroxy-12(Z)-octadecenoic acid (10-OHODA) has an inhibitory effect on the tension of papillary muscles in isolated guinea-pig hearts. To establish an immunoassay for 10-OHODA a mouse monoclonal antibody (MoAb), YM-1, was produced. In order to evaluate the ability of this MoAb to recognize various 10-OHODA analogs including leukotoxin (9, 10-epoxy-12-octadecenoic acid, LTx), a sensitive enzyme-linked immunosorbent assay (ELISA) was developed using the avidin-biotin complex (ABC). The detection limit for 10-OHODA was as low as 0.5 ng in this system. In order to demonstrate the presence of 10-OHODA in living cells, macrophages derived from the human leukemia cell line THP-1 by adding 160nM phorbol 12-myristate 13-acetate (PMA) were exposed to 95% O2, and 5% CO2 for 24 h. 10-OHODA and other fatty acids were extracted from the exposed macrophages with diethylether after phospholipase A2 treatment. The 10-OHODA content was determined using the new ELISA, and 18.5 ng 10-OHODA was detected in the macrophages exposed to the high oxygen concentration (1 x 10(6) cells).","PeriodicalId":16060,"journal":{"name":"Journal of immunoassay","volume":"18 4","pages":"309-19"},"PeriodicalIF":0.0,"publicationDate":"1997-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01971529708005824","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20288248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of monoclonal antibodies against Nosema bombycis and their utility for detection of pebrine infection in Bombyx mori L. 家蚕微孢子虫单克隆抗体的制备及其在家蚕微孢子虫感染检测中的应用。

Journal of immunoassay Pub Date : 1997-11-01 DOI: 10.1080/01971529708005827

M Shamim, D Ghosh, M Baig, B Nataraju, R K Datta, S K Gupta

{"title":"Production of monoclonal antibodies against Nosema bombycis and their utility for detection of pebrine infection in Bombyx mori L.","authors":"M Shamim, D Ghosh, M Baig, B Nataraju, R K Datta, S K Gupta","doi":"10.1080/01971529708005827","DOIUrl":"https://doi.org/10.1080/01971529708005827","url":null,"abstract":"Latex agglutination assay based on monoclonal antibodies (MCAs) described in this communication may be useful for detection of Pebrine infection in silkworm. Four murine MCAs were produced against Nosema bombycis spore. In ELISA all 4 MCAs (IgM isotype) reacted with alkali treated Nosema spores and to variable extent with acetone precipitated surface protein. However, MA-310 and MA-542 showed a low degree of cross reactivity with BmNPV. In contrast, MA-503 and MA-515 were devoid of reactivity with BmNPV, B. thuringiensis, S. marcescens, Azotobactor, Rhizobium and normal hemolymph protein in ELISA. Latex beads sensitized with a combination of MA-503 and MA-515 (50 micrograms each per ml of 0.4% latex beads) could detect 1 x 10(5) Nosema spores per test. Sensitization of the latex beads with the cocktail of these two MCAs through protein-A bridge further led to a 10-fold increase in the sensitivity (1 x 10(4) spores/test) of the assay. No agglutination was observed in presence of BmNPV, Rhizobium, Azotobactor, E. coli, B. thuringiensis, S. marcescens and normal hemolymph protein indicating the specificity of the test. The results obtained by latex agglutination assay on hemolymph samples of infected as well as normal larvae collected from field, II instar larvae infected in the laboratory and from infected mother moth revealed 100% correlation with results by microscopic examination.","PeriodicalId":16060,"journal":{"name":"Journal of immunoassay","volume":"18 4","pages":"357-70"},"PeriodicalIF":0.0,"publicationDate":"1997-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01971529708005827","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20288251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Bispecific rabbit Fab'-bovine serum albumin conjugate used in hemagglutination immunoassay for beta-microseminoprotein. 双特异性兔Fab′-牛血清白蛋白偶联物用于β -微精球蛋白血凝免疫测定。

Journal of immunoassay Pub Date : 1997-08-01 DOI: 10.1080/01971529708005816

N Yukawa, M Osawa, T Saito, T Hasegawa, H Matsuda, K Takahama, S Takeichi

引用次数: 1

Development of a specific fluoroimmunoassay for ovine albumin. 羊白蛋白特异性荧光免疫测定方法的建立。

Journal of immunoassay Pub Date : 1997-08-01 DOI: 10.1080/01971529708005815

D H Hreczuk-Hirst, K Harrison, A Sidki

引用次数: 0

Quantification of cell-associated and free antigens in Bordetella pertussis suspensions by antigen binding ELISA. 抗原结合ELISA法定量百日咳杆菌悬液中细胞相关抗原和游离抗原。

Journal of immunoassay Pub Date : 1997-08-01 DOI: 10.1080/01971529708005819

J Westdijk, J van den Ijssel, M Thalen, C Beuvery, W Jiskoot

{"title":"Quantification of cell-associated and free antigens in Bordetella pertussis suspensions by antigen binding ELISA.","authors":"J Westdijk, J van den Ijssel, M Thalen, C Beuvery, W Jiskoot","doi":"10.1080/01971529708005819","DOIUrl":"https://doi.org/10.1080/01971529708005819","url":null,"abstract":"In order to achieve batch-to-batch consistency of whole-cell pertussis vaccines, properties relevant for protection and safety should be characterised. Therefore, ELISAs to quantify pertussis toxin (PT), filamentous haemagglutinin (FHA), 92 kD outer membrane protein (92 kD-OMP) and pertactin (PRN) in Bordetella pertussis (B. pertussis) suspensions were developed. In this paper the influence of the bacterial growth stage on antigen production and antigen release into the supernatant was studied for pertussis strains 134, 509 and CS. The levels of cell-associated and free antigens during growth were strongly strain and antigen dependent. Because of this, the proportion of cell-associated antigens changed during cultivation for all three strains. Substantial amounts of PT and PRN were released into the supernatant, while little free FHA and 92 kD-OMP were found. The amount of cell-associated FHA declined rapidly during growth, whereas cell-associated 92 kD-OMP contents increased. These findings demonstrate that, although antigen exposure and release differ from strain to strain, the main factor that determines the antigen production and release is the growth phase.","PeriodicalId":16060,"journal":{"name":"Journal of immunoassay","volume":"18 3","pages":"267-84"},"PeriodicalIF":0.0,"publicationDate":"1997-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01971529708005819","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20198561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Water immiscible solvent based immunoassay. 基于水不混溶溶剂的免疫测定。

Journal of immunoassay Pub Date : 1997-08-01 DOI: 10.1080/01971529708005817

J P Aston, E J Hitchings, R L Ball, I Weeks, J S Woodhead

引用次数: 3

Sensitive human thyrotropin immunoradiometric assay set up by the identification and minimization of nonspecific bindings. 通过识别和最小化非特异性结合建立敏感的人促甲状腺素免疫放射测定法。

Journal of immunoassay Pub Date : 1997-08-01 DOI: 10.1080/01971529708005818

C N Peroni, M T Ribela, P Bartolini

{"title":"Sensitive human thyrotropin immunoradiometric assay set up by the identification and minimization of nonspecific bindings.","authors":"C N Peroni, M T Ribela, P Bartolini","doi":"10.1080/01971529708005818","DOIUrl":"https://doi.org/10.1080/01971529708005818","url":null,"abstract":"An immunoradiometric assay (IRMA) of human thyrotropin (hTSH), based on magnetic solid phase separation, was studied especially in terms of its nonspecific bindings (B0) which were identified as a product of the interaction between an altered form of radioiodinated anti-hTSH monoclonal antibody (125I-mAB) and the uncoupled magnetizable cellulose particle (matrix). Preincubation with the same matrix, solid phase saturation with milk proteins, tracer storage at 4 degrees C and serum addition during incubation were found to be particularly effective in preventing their formation. These findings were used to reproducibly decrease nonspecific bindings to values < 0.1% (or < 70 cpm), thus increasing the signal-to-noise ratio (B60/B0) up to values of 300-500. This way hTSH radioassays were obtained with functional sensitivities of about 0.05 mIU/L and analytical sensitivities of the order of 0.02 mIU/L. Such sensitivities, and, more importantly, a general improvement in assay performance, were obtained in a highly reproducible manner and all over the useful tracer life.","PeriodicalId":16060,"journal":{"name":"Journal of immunoassay","volume":"18 3","pages":"247-65"},"PeriodicalIF":0.0,"publicationDate":"1997-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01971529708005818","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20198560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Direct radioimmunoassay of progesterone in saliva. 唾液中黄体酮的直接放射免疫测定。

Journal of immunoassay Pub Date : 1997-05-01 DOI: 10.1080/01971529708005810

Y C Lu, R T Chatterton, K M Vogelsong, L K May

引用次数: 32

Application of an enzyme immunoassay to monitor bacterial binding and to measure inhibition of binding to different types of solid surfaces. 应用酶免疫测定法监测细菌结合和测量不同类型固体表面的结合抑制。

Journal of immunoassay Pub Date : 1997-05-01 DOI: 10.1080/01971529708005811

Y el Tahir, P Toivanen, M Skurnik

引用次数: 3

Temperature-dependent non-specific adsorption of recombinant bovine leukemia virus receptor BLVRcp1 in immunoassay. 重组牛白血病病毒受体BLVRcp1在免疫分析中的温度依赖性非特异性吸附。

Journal of immunoassay Pub Date : 1997-05-01 DOI: 10.1080/01971529708005812

O Orlik, C Altaner, G A Splitter

引用次数: 1