Journal of immunopharmacology最新文献

{"title":"Effect of cyclophosphamide on T-lymphocyte marker expression in T-cell areas of some lymphoid organs of the rat.","authors":"E el-Sady,&nbsp;A V Antoniou,&nbsp;D Parker,&nbsp;J L Turk","doi":"10.3109/08923978609026499","DOIUrl":"https://doi.org/10.3109/08923978609026499","url":null,"abstract":"<p><p>Surface markers on rat lymphocytes from thymus and T-cell areas of lymph node, spleen and Peyer's patches were examined in histological sections after one single dose of Cyclophosphamide (CY, 100 mg/kg body weight). A panel of monoclonal antibodies was used: W3/13, W3/25 and OX8 to investigate Pan T, TH and Ts/c marker expressions respectively. Ts/c marker expression showed a marked and significant reduction in both thymus and lymph node lymphocytes 3 days after CY treatment. This was followed by return to normal in the thymus and a significant rebound increase in the lymph node by day 7 after treatment. This Ts/c marker expression in both the spleen and Peyer's patches showed a significant increase on both day 3 and 7 after CY treatment. Mast cells were observed in large numbers in the thymus and lymph node but not in the spleen and Peyer's patches. TH marker expression was increased significantly in lymph nodes, spleen and Peyer's patches. No change was observed in Pan T marker expression in any of the tissues at any of the times examined.</p>","PeriodicalId":16049,"journal":{"name":"Journal of immunopharmacology","volume":"8 4","pages":"439-53"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/08923978609026499","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14017517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Some new aspects in autoimmunity.","authors":"U Bicker","doi":"10.3109/08923978609026505","DOIUrl":"https://doi.org/10.3109/08923978609026505","url":null,"abstract":"AbstractProf. Dr. med. W. Schaumann dedicated on the occasion of his 60th birthday on November 20, 1986The interaction of T-helper cells and autoreactive B-lymphocytes in the initiation of autoimmune processes is discussed resulting in the hypothesis that this interaction not only leads to the production of autoantibodies but also to the induction of cellular autoimmune processes. Based on this hypothesis, the immunopharmacological properties of a new immunomodulating drug, Ciamexone, are discussed.","PeriodicalId":16049,"journal":{"name":"Journal of immunopharmacology","volume":"8 4","pages":"543-59"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/08923978609026505","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14083277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Secretory leukemic B cells express T cell markers in vitro. A phenomenon suppressed by TPA.","authors":"A P Efremidis,&nbsp;H S Haubenstock,&nbsp;N M Papadopoulos,&nbsp;J F Holland,&nbsp;J G Bekesi","doi":"10.3109/08923978609028612","DOIUrl":"https://doi.org/10.3109/08923978609028612","url":null,"abstract":"<p><p>Immunological and biochemical markers of leukemia/lymphoma cells have provided valuable insight into hematopoietic malignancy and normal differentiation. The general assumption is that as early lymphoid cells become committed towards terminal differentiation they lose their capacity for bimodal differentiation and cells become restricted to B or T cell development and function. We have observed that phenotypically \"late\" leukemic B cells close to secretory stage can spontaneously express mature T cell antigens (T11, T4 and T8) after culture in vitro. In further studies of these cells, it was found that the biochemical marker lactate Dehydrogenase (LD) follows the intermediate pattern expressed by thymocytes rather than that of typical B cells. The expression of T cell antigens can be blocked by incubating these cells with the phorbol ester TPA (12-0-tetradecanoyl phorbol 13 acetate) which promotes unidirectional B cell maturation to plasmacytoid cells in a way that mimics normal B cell differentiation. These observations indicate that presecretory malignant B cells are still programmed to express T cell biochemical and antigenic markers and this expression can be influenced by environmental conditions in vitro.</p>","PeriodicalId":16049,"journal":{"name":"Journal of immunopharmacology","volume":"8 2","pages":"129-44"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/08923978609028612","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14611484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Accumulation of immature B and null lymphocytes in the periphery after intraperitoneal administration of traditional Chinese medicine, xiao-chai-hu-tang (Japanese name: shosaiko-to).","authors":"T Kawakita,&nbsp;A Yamada,&nbsp;Y Kumazawa,&nbsp;K Nomoto","doi":"10.3109/08923978609026506","DOIUrl":"https://doi.org/10.3109/08923978609026506","url":null,"abstract":"<p><p>Accumulation of lymphocytes after an intraperitoneal (ip) injection of a traditional Chinese herb medicine, XIAO-CHAI-HU-TANG (Japanese name: shosaiko-to), was investigated. Shosaiko-to induced marked accumulation of lymphocytes rather than macrophages in the peritoneal cavity of ICR mice, whereas various kinds of irritants, e.g. proteose-pepton, Escherichia coli lipopolysaccharide (LPS), OK-432 and Corynebacterium parvum, induced preferential accumulation of macrophages rather than lymphocytes. By means of analysis using two-color fluorescence-activated cell sorter (FACS), it was revealed that the increased lymphocyte subpopulations not only in the peritoneal cavity but also in the spleen of C3H/He mice by the injection of shosaiko-to were comprised of both immature B (IgM+ and IgD-) and null (thyl- and Ig-) cells. This effect of shosaiko-to was observed in other C5 normal strains, C3H/HeJ (LPS-nonresponder), C57BL/6, BALB/c and athymic nu/nu (ICR background) mice, but not in C5 deficient strains, AKR/J, A/J and DBA/2 mice, indicating that the accumulation of immature B and null cells in the periphery induced by shosaiko-to is closely related to the complement system.</p>","PeriodicalId":16049,"journal":{"name":"Journal of immunopharmacology","volume":"8 4","pages":"561-88"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/08923978609026506","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14615784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low molecular weight immunosuppressive factors found in elevated amounts in cancer ascitic fluids of mice. 1. Isolation, identification and immunosuppressive effects of uric acid and uracil.","authors":"S Sami,&nbsp;S Takano,&nbsp;T Majima,&nbsp;H Aso,&nbsp;T Nakamura,&nbsp;N Ishida","doi":"10.3109/08923978609031084","DOIUrl":"https://doi.org/10.3109/08923978609031084","url":null,"abstract":"<p><p>A definite increase in two low molecular weight factors, G10-2 and G10-3 was found in Ehrlich ascitic fluids, parallel to tumor growth. The isolation and identification of the two factors were attempted through gel filtration and reversed phase column chromatography, using ascitic fluids obtained 13 days after intraperitoneal implantation of Ehrlich tumor cells. As a result, two highly purified factors were observed upon examination by high performance liquid chromatography. Additional analytical data, collected by UV spectrum, NMR spectrum and mass analysis, allowed us to identify G10-2 as uric acid and G10-3 as uracil. Detailed immunological analysis of uric acid and uracil revealed that the augmenting activities of mouse and human NK cells by mouse IFN alpha/beta or human rIFN alpha A/D were impaired in the presence of either compound at concentrations of 0.07 mM, the concentration detectable in the ascitic fluid of tumor bearing mice.</p>","PeriodicalId":16049,"journal":{"name":"Journal of immunopharmacology","volume":"8 1","pages":"39-58"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/08923978609031084","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14831309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antiviral activity of Bordetella pertussis vaccine-elicited peritoneal exudate cells.","authors":"D W Baggett,&nbsp;P A LeBlanc,&nbsp;F S Allison,&nbsp;M J Thomley,&nbsp;A L Winters","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Peritoneal exudate cells collected from mice 7 days after treatment with Bordetella pertussis vaccine exhibited significant in vitro antiviral activity against vesicular stomatitis virus (VSV). Vaccine-induced peritoneal exudate cells exhibited both intrinsic and extrinsic antiviral activity in culture with target VSV-infected L cells. Virus replication was poor in the vaccine-induced exudate cells. Coculture of vaccine-induced exudate cells and VSV-infected L cell targets decreased virus yield. The activity appeared specific for infected cells and at least a portion of the antiviral activity was directed against the initial infection cycle. Nonadherent vaccine-induced exudate cells showed an increase in antiviral activity over total vaccine-induced exudate cells.</p>","PeriodicalId":16049,"journal":{"name":"Journal of immunopharmacology","volume":"8 4","pages":"589-609"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14160302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Humoral and cellular immunologic aspects of adjuvant and collagen arthritis in rats.","authors":"N Panosian,&nbsp;S Heyner,&nbsp;R J Capetola,&nbsp;R F Orzechowski","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Systemic and local immunological responses of rats sensitized with either M. butyricum or native type II collagen have been evaluated. In rats exhibiting adjuvant-induced arthritis no antibodies to collagen could be detected. In animals exhibiting collagen-induced arthritis, high antibody titers developed by day 14, and could be correlated with the severity of the arthritis. Delayed type hypersensitivity (DTH) responses were measured by a 5-iodo-2'-deoxyuridine 125-I (125-IUdR) uptake assay. Arthritic scores in rats immunized with collagen were not accompanied by a positive DTH response, whereas adjuvant arthritic rats showed a positive response. T-lymphocyte cellular responses in both adjuvant- and collagen-induced arthritic rats were measured. In neither syndrome were major alterations observed in T-lymphocyte subpopulations. These results provide evidence that adjuvant-induced arthritis and type II collagen-induced arthritis are distinct entities, and that they may be discriminated by the nature of the humoral response.</p>","PeriodicalId":16049,"journal":{"name":"Journal of immunopharmacology","volume":"8 3","pages":"347-70"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14759311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic effect of endogenous tumor necrosis factor on ascites Meth A sarcoma.","authors":"N Watanabe,&nbsp;Y Niitsu,&nbsp;H Sone,&nbsp;H Neda,&nbsp;I Urushizaki,&nbsp;A Yamamoto,&nbsp;M Nagamuta,&nbsp;Y Sugawara","doi":"10.3109/08923978609028619","DOIUrl":"https://doi.org/10.3109/08923978609028619","url":null,"abstract":"<p><p>The therapeutic effect of endogenous tumor necrosis factor (TNF) on Meth A ascites fibrosarcoma in mice was investigated. Serum and peritoneal fluid from tumor bearing mice treated with OK-432 and LPS were cytotoxic to tumor cells in vitro. The peak of cytotoxicity in both the serum and peritoneal fluid was found in the fraction corresponding to a molecular weight of approximately 54,000-56,000 on HPLC and the pI was found to be 4.9-5.1 by isoelectric focusing. These results are consistent with previously reported findings on TNF, and indicate that endogenous TNF has a satisfactory life-prolonging effect. The tumor necrosis factor (TNF) is considered to be one of the clinically most promising anti-cancer cytokines because of its potent and very specific antitumor effect on target cells (Carswell, Old, Kassel, Green, Fiore & Williamson, 1975; Matthews & Watkins, 1978; Niitsu, Watanabe & Urushizaki, 1984). TNF as an anti-cancer cytokine for the treatment of cancer may be applied in one of the two following ways: by administration of purified TNF or by endogenously inducing TNF in cancer bearing individuals. The antitumor effects of TNF administered exogenously have been examined using crude preparations or serum containing TNF (tumor necrosis serum, TNS) (Carswell et al., 1975; Watanabe, Niitsu, Sone, Neda, Ishigaki & Urushizaki, 1984). In a previous paper we reported that mice primed with OK-432 and challenged with endotoxin produced a soluble cytotoxic factor in peritoneal fluids (Yamamoto, Nagamuta, Usami, Sugawara, Watanabe, Niitsu & Urushizaki, 1985; Nagamuta, Yamamoto, Usami, Sugawara, Watanabe, Niitsu & Urushizaki, 1985). Ths peritoneal cytotoxic factor (PCF) had cytostatic and/or cytotoxic effect not only on mouse tumor cell lines but also on human tumor cell lines without species specificity. Normal cell lines were not affected. Here we report the endogenous production of TNF in tumor bearing mice and its antitumor effects.</p>","PeriodicalId":16049,"journal":{"name":"Journal of immunopharmacology","volume":"8 2","pages":"271-83"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/08923978609028619","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14841814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Angiotensin II suppression of human mononuclear cell reactivity is associated with enhanced OKT8+ lymphocyte thymidine incorporation.","authors":"M R Simon,&nbsp;D E Engel,&nbsp;J V Weinstock","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Recent evidence suggests that angiotensin II may participate in the regulation of inflammation. We previously reported that angiotensin II inhibits human peripheral blood mononuclear cell reactivity and acts directly on lymphocytes. These observations are again confirmed. In addition, purified OKT8+ but not OKT4+ T cell suspensions stimulated with phytohemagglutinin revealed increased thymidine incorporation when simultaneously cultured for 48 hours with angiotensin II. These findings suggest that angiotensin II may inhibit mononuclear cell thymidine uptake through stimulation of suppressor lymphocytes contained within the OKT8+ subpopulation.</p>","PeriodicalId":16049,"journal":{"name":"Journal of immunopharmacology","volume":"8 3","pages":"289-97"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14613994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological and functional changes in mouse splenic lymphocytes following in vivo and in vitro exposure to chlorphentermine.","authors":"L. Sauers, D. Wierda, E. Walker, M. Reasor","doi":"10.3109/08923978609026508","DOIUrl":"https://doi.org/10.3109/08923978609026508","url":null,"abstract":"With repeated administration to animals, the cationic, amphiphilic drug, chlorphentermine (CP), has been shown by others to induce a phospholipidosis in lymphocytes. In the present study mouse splenic lymphocytes, exposed to CP, either in vivo or in vitro, developed morphological changes consistant with the induction of phospholipidosis. In addition, CP induced functional changes in lymphocytes. Mice, treated with CP in vivo, demonstrated a significantly depressed ability to generate a delayed hypersensitivity response or to produce antibody-secreting cells against de novo antigens. Mouse splenic lymphocytes, exposed to 10(-7) M CP for 3 days in vitro, demonstrated a significantly depressed blastogenic response to the mitogens phytohemagglutinin, concanavalin A and lipopolysaccharide. CP inhibited an event that occurred early during lymphocyte activation, but was subsequent to mitogen/receptor coupling. In addition, CP significantly depressed the increased uptake of choline that occurs in lymphocytes following cellular activation. Since the presence of phospholipidosis is indicative of an impairment in phospholipid metabolism, these results taken together provide evidence for a relationship between this phenomenon and altered immune function.","PeriodicalId":16049,"journal":{"name":"Journal of immunopharmacology","volume":"24 1","pages":"611-31"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85869430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}