Journal of Food Science and Technology-mysore最新文献

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Rapid recognition of origins and ages of Rice wine based on Tri-step IR and electronic tongue 基于三步红外和电子舌的米酒起源和年代的快速识别
IF 3.1 3区 农林科学
Journal of Food Science and Technology-mysore Pub Date : 2018-06-14 DOI: 10.25177/JFST.3.3.3
Changhua Xu, Yu Yan, Xiao-peng Zhang, Jia-juan Huang, Xiao-zhen Hu, Jian-Hong Gan, Zhengquan Wang, Ningping Tao, Xichang Wang, Sift Desk Journals Open Access Journals
{"title":"Rapid recognition of origins and ages of Rice wine based on Tri-step IR and electronic tongue","authors":"Changhua Xu, Yu Yan, Xiao-peng Zhang, Jia-juan Huang, Xiao-zhen Hu, Jian-Hong Gan, Zhengquan Wang, Ningping Tao, Xichang Wang, Sift Desk Journals Open Access Journals","doi":"10.25177/JFST.3.3.3","DOIUrl":"https://doi.org/10.25177/JFST.3.3.3","url":null,"abstract":"Rice wine, an ancient drink of China, commonly has different origins and wine ages according to traditional processing methods, inducing great challenges to be authenticated and discriminated rapidly by conventional procedures. This study sought to develop an easy and reliable method, based on a Tri-step infrared spectroscopy, combined with electronic sensory for accurate and rapid discrimination of rice wine (RW). The class of nutrition (lipids, protein and other compounds), wine ages and origins were step-wisely evaluated and nutrition profile variations among different samples were integrated and interpreted by fast identification of their relative contents, based on spectral peak intensity. 140 batches of RW (twenty for each samples) were effectively classified by IR-PCA. In the sensory aspect, an electronic tongue distinguished rice wine objectively in taste with discrimination indexes that were greater than 95 for all the samples. The developed method would be an economic and simple approach for rapid chemotaxonomic discrimination of","PeriodicalId":16004,"journal":{"name":"Journal of Food Science and Technology-mysore","volume":"1 1","pages":"1-8"},"PeriodicalIF":3.1,"publicationDate":"2018-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79793515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Microbiological analysis of lettuce (Lactuca sativa) from conventional and organic cultivation commercialized at fairs in Brazil 在巴西集市上商业化的传统和有机种植莴苣(Lactuca sativa)的微生物分析
IF 3.1 3区 农林科学
Journal of Food Science and Technology-mysore Pub Date : 2018-06-11 DOI: 10.25177/JFST.3.2.4
T. Gandra, Pâmela Inchauspe Corrêa Alves, E. A. Gandra, Sift Desk Journals Open Access Journals
{"title":"Microbiological analysis of lettuce (Lactuca sativa) from conventional and organic cultivation commercialized at fairs in Brazil","authors":"T. Gandra, Pâmela Inchauspe Corrêa Alves, E. A. Gandra, Sift Desk Journals Open Access Journals","doi":"10.25177/JFST.3.2.4","DOIUrl":"https://doi.org/10.25177/JFST.3.2.4","url":null,"abstract":"Brazilian laws established regulatory limits in lettuce only to thermotolerant coliforms (fecal coliforms) and coagulase-positive Staphylococci of 10 MPN g and 10 CFU g respectively. The objective of this study was to evaluate and compare contamination of lettuce from conventional and organic cultivation, commercialized at fairs in south of Brazil, by the fecal coliforms, coagulase-positive Staphylococci, Salmonella spp. and Listeria monocytogenes. Eighty samples of traditionally and organically produced lettuce each were collected. The contaminated samples percent were 35 and 71.25, for fecal coliforms, 35 and 32,5 for coagulase-positive Staphylococci, 0 and 5 for Salmonella spp., 2.5 and 2.5 for Listeria monocytogenes, in conventional lettuce and organic lettuce, respectively. Results indicated that 35% of the samples of conventionally produced lettuce and 71.25% of the samples of organically produced one presented fecal coliform count above regulatory limits. Coagulasepositive Staphylococci counts above regulatory limits were verified in 28 and 26 samples from the conventional and organic agriculture, respectively. Salmonella spp. was present in 5% of organically produced samples and L. monocytogenes in 2.5% of lettuce samples from both types of agricultures. Obtained results highlight the importance of good practices from cultivation to commercialization to improve the hygienic-sanitary quality of vegetables.","PeriodicalId":16004,"journal":{"name":"Journal of Food Science and Technology-mysore","volume":"6 1","pages":"1-8"},"PeriodicalIF":3.1,"publicationDate":"2018-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85370779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Thiol-Cyclodextrin: A New Agent For Controlling The Catalytic Activity Of Polyphenol Oxidase From Red Delicious Apple 巯基环糊精:控制红鲜苹果多酚氧化酶催化活性的新药剂
IF 3.1 3区 农林科学
Journal of Food Science and Technology-mysore Pub Date : 2018-05-04 DOI: 10.25177/jfst.3.2.2
K. Ovsejevi, Gabriela Peralta-Altier, C. Manta, Sift Desk Journals Open Access Journals
{"title":"Thiol-Cyclodextrin: A New Agent For Controlling The Catalytic Activity Of Polyphenol Oxidase From Red Delicious Apple","authors":"K. Ovsejevi, Gabriela Peralta-Altier, C. Manta, Sift Desk Journals Open Access Journals","doi":"10.25177/jfst.3.2.2","DOIUrl":"https://doi.org/10.25177/jfst.3.2.2","url":null,"abstract":"Polyphenol oxidase (PPO, EC 1.14.18.1) is the main enzyme responsible for enzymatic browning, a natural process which produces deterioration of fruits and vegetables. One alternative to prevent this undesirable process is to inhibit the catalytic activity of PPO by encapsulating enzyme’s substrates in cyclodextrins (CD). In this article the effect of a Thiol-CD on PPO from Red Delicious apple was studied, demonstrating that this compound is a powerful tool for controlling oxidative processes in food. Thiol-CD could encapsulate the polyphenols, natural substrates of the enzyme, by means of the cyclodextrin hydrophobic internal cavity. Simultaneously, through the thiol group, it could inactivate the PPO by reducing the copper ions from the active site of the enzyme. Moreover, thiol moieties could decrease the browning by reducing the quinones generated by oxidative processes. The isolation and purification of PPO from apple was performed in order to study those effects, different polyphenols, chlorogenic acid (CA) and 4-methylcatechol (4-MC), were assayed as enzymatic substrates. Both β -CD and Thiol-CD exhibited better enzyme inhibition value for CA than for 4-MC. Moreover, Thiol-CD showed an extraordinary performance compared with β-CD. When CA 10 mM was used, 5 mM β-CD gave 11 % PPO inhibition, meanwhile nearly 100 times less concentration of Thiol-CD (45 μM) gave 100 % of enzyme inhibition.","PeriodicalId":16004,"journal":{"name":"Journal of Food Science and Technology-mysore","volume":"43 1","pages":"1-8"},"PeriodicalIF":3.1,"publicationDate":"2018-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76102534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Suitable preanalytical conditions for vitamin C measurement in clinical routine 临床常规测定维生素C的适宜分析前条件
IF 3.1 3区 农林科学
Journal of Food Science and Technology-mysore Pub Date : 2018-05-03 DOI: 10.25177/JFST.3.2.3
C. Steuer, L. Bernasconi, C. Saxer, P. Neyer, A. Huber, Sift Desk Journals Open Access Journals
{"title":"Suitable preanalytical conditions for vitamin C measurement in clinical routine","authors":"C. Steuer, L. Bernasconi, C. Saxer, P. Neyer, A. Huber, Sift Desk Journals Open Access Journals","doi":"10.25177/JFST.3.2.3","DOIUrl":"https://doi.org/10.25177/JFST.3.2.3","url":null,"abstract":"Pre-analytical sampling conditions play an important role for the correct determination of blood concentrations of biomarkers and drugs in the clinical environment. In the context of daily clinical routine and clinical studies, oxidative stability in vitro should be carefully considered. Herein we report differences in vitamin C levels under commonly used handling and storage conditions and we propose a simple and efficient sampling protocol. Plasma and serum samples were analyzed by HPLC-UV and different handling-, timeand storage conditions were investigated in detail. Our findings show that protection from oxidation significantly prevented degradation under different storage conditions. The immediate separation of stabilized plasma from blood cells showed a protective effect on vitamin C stability. In addition, long-term storage of vitamin C was positively influenced by addition of protective agents, assuring stability up to six months. Oxidative degradation could affect not only vitamin C but also other small molecule metabolites and should therefore be intensively evaluated before implementation in daily clinical routine as well as in metabolomics studies.","PeriodicalId":16004,"journal":{"name":"Journal of Food Science and Technology-mysore","volume":"36 1","pages":"1-8"},"PeriodicalIF":3.1,"publicationDate":"2018-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88554635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
The challenge of monitoring the hydrolysis of foods lipids during gastrointestinal digestion 在胃肠道消化过程中监测食物脂质水解的挑战
IF 3.1 3区 农林科学
Journal of Food Science and Technology-mysore Pub Date : 2018-04-19 DOI: 10.25177/jfst.3.1.3
M. Guillén, Bárbara Nieva-Echevarría, E. Goicoechea, Sift Desk Journals Open Access Journals
{"title":"The challenge of monitoring the hydrolysis of foods lipids during gastrointestinal digestion","authors":"M. Guillén, Bárbara Nieva-Echevarría, E. Goicoechea, Sift Desk Journals Open Access Journals","doi":"10.25177/jfst.3.1.3","DOIUrl":"https://doi.org/10.25177/jfst.3.1.3","url":null,"abstract":"During the last decade obtaining further knowledge on lipid digestion has become a challenging task in the field of Food Science and Nutrition research. However, the great complexity of this process requires the use of sound, accurate and simple analytical techniques which are able to provide as much information as possible; only thus can a global view and therefore a better understanding of the ongoing process be obtained. This review tackles the advantages and drawbacks of the different methodologies currently employed for this purpose, focusing on a new approach, recently developed and based on Proton Nuclear Magnetic Resonance (H NMR) spectroscopy. This new methodological approach not only provides a great deal of information in a simple, rapid and accurate way, but also overcomes many of the disadvantages of the techniques employed to date. In this sense, H NMR can be considered a very promising alternative for research on lipid digestion, contributing to shed more light on the complex digestion process of lipids and the factors that may affect it.","PeriodicalId":16004,"journal":{"name":"Journal of Food Science and Technology-mysore","volume":"60 1","pages":"1-8"},"PeriodicalIF":3.1,"publicationDate":"2018-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81153766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A colorimetric assay using tetrazolium salts with an electron mediator to evaluate yeast growth in opaque dispersed systems 用四氮唑盐和电子介质评价酵母在不透明分散体系中的生长的比色试验
IF 3.1 3区 农林科学
Journal of Food Science and Technology-mysore Pub Date : 2018-04-11 DOI: 10.25177/JFST.3.1.4
C. A. Campos, Aldana L Zalazar, Malena M González, M. F. Gliemmo, Sift Desk Journals Open Access Journals
{"title":"A colorimetric assay using tetrazolium salts with an electron mediator to evaluate yeast growth in opaque dispersed systems","authors":"C. A. Campos, Aldana L Zalazar, Malena M González, M. F. Gliemmo, Sift Desk Journals Open Access Journals","doi":"10.25177/JFST.3.1.4","DOIUrl":"https://doi.org/10.25177/JFST.3.1.4","url":null,"abstract":"Background: Traditional microbiological enumeration techniques such as colony count are time consuming. Visual evaluation of turbidity growth lacks objectivity and precision. On the other hand, the accuracy of the spectrophotometric readings can be limited by media opacity and/or additives. In this context, colorimetric methods based on tetrazolium salts are an alternative to traditional methods due to their ability to generate endpoints based on color change. The objective of this study was to develop a colorimetric assay based on two different tetrazolium salts and an electron mediator in order to replace the conventional method of viable plate count or absorbance for microbial growth detection in opaque dispersed systems. Methods: A color imetr ic method to evaluate yeast growth in broth and opaque dispersed systems using two tetrazolium salts, 2,3,5-triphenyl-2H-tetrazolium chloride (TTC), 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5 -phenyl-2H-tetrazolium chloride (INT) and an electron mediator, 2-methoxy-1,4-Naphthoquinone (MNQ) was developed. In addition, the minimum inhibitory concentrations against Zygosaccharomyces bailii for natamycin and lemongrass essential oil were determined by the proposed method and compared with those obtained using the conventional broth microdilution and plate count methods. Results: Showed that the mixture of INT/MNQ, was more effective than TTC/MNQ. In opaque structure systems, the later showed to be more sensitive than absorbance However, in broth it was as sensitivity as absorbance to detect yeast growth. Besides, the INT/MNQ reagent functioned adequately to determine the minimum inhibitory concentrations of antimicrobials in opaque structured systems. Conclusions: These results demonstrated that the proposed method functioned adequately to determine the minimum inhibitory concentrations of antimicrobials in opaque structured systems showing an excellent agreement with the plate count method and the proposed technique allowed the evaluation of yeast growth with lower population levels than the ones required through absorbance readings. Moreover, this colorimetric method is easy to perform and less time consuming.","PeriodicalId":16004,"journal":{"name":"Journal of Food Science and Technology-mysore","volume":"133 1 1","pages":"1-8"},"PeriodicalIF":3.1,"publicationDate":"2018-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73337040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Replacement meal to treat overweight patients 替代餐治疗超重患者
IF 3.1 3区 农林科学
Journal of Food Science and Technology-mysore Pub Date : 2018-03-15 DOI: 10.25177/JFST.3.1.2
Volpe Roberto, Pacioni Fabrizio, Sorce Maria Luisa, Todisco Miriam, Rome, C. Rome, intern student at Cnr. Vr conception, drafting, Tm, P. A. Moro, Sift Desk Journals Open Access Journals
{"title":"Replacement meal to treat overweight patients","authors":"Volpe Roberto, Pacioni Fabrizio, Sorce Maria Luisa, Todisco Miriam, Rome, C. Rome, intern student at Cnr. Vr conception, drafting, Tm, P. A. Moro, Sift Desk Journals Open Access Journals","doi":"10.25177/JFST.3.1.2","DOIUrl":"https://doi.org/10.25177/JFST.3.1.2","url":null,"abstract":"","PeriodicalId":16004,"journal":{"name":"Journal of Food Science and Technology-mysore","volume":"40 1","pages":"1-8"},"PeriodicalIF":3.1,"publicationDate":"2018-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79887176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
PLANT DISEASE DETECTION BY IMAGE PROCESSING: A LITERATURE REVIEW 植物病害图像处理检测的文献综述
IF 3.1 3区 农林科学
Journal of Food Science and Technology-mysore Pub Date : 2018-01-01 DOI: 10.25177/jfst.3.6.6
Monishanker Halder, A. Sarkar, H. Bahar, Sift Desk Journals Open Access Journals
{"title":"PLANT DISEASE DETECTION BY IMAGE PROCESSING: A LITERATURE REVIEW","authors":"Monishanker Halder, A. Sarkar, H. Bahar, Sift Desk Journals Open Access Journals","doi":"10.25177/jfst.3.6.6","DOIUrl":"https://doi.org/10.25177/jfst.3.6.6","url":null,"abstract":"To promote sustainable development, the smart city implies a global vision that merges artificial intelligence, big data, decision making, information and communication technology (ICT), and the Internet-of -Things (IOT). These processes above are related for solving real life problems. Food is one of the basic needs of human being. World population is increasing day by day. So it has become important to grow sufficient amount of crops to feed such a huge population. But with the time passing by, plants are affected with various kinds of diseases, which cause great harm to the agricultural plant productions. Beside that many countries economy greatly depends on agricultural productivity and it’s also a need for a coun try to attain agricultural productivity of basic agricultural product for the people of that particular country. Detection of plant disease through some automatic technique is beneficial as it requires a large amount of work of monitoring in big farm of crops, and at very early stage itself it detects symptoms of diseases means where they appear on plant leaves. In this paper surveys on different disease classification techniques that can be used for plant leaf disease detection.","PeriodicalId":16004,"journal":{"name":"Journal of Food Science and Technology-mysore","volume":"13 1","pages":"534-538"},"PeriodicalIF":3.1,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72532115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 19
柚子精油提取、成分、贮藏及应用研究进展 柚子精油提取、成分、贮藏及应用研究进展
IF 3.1 3区 农林科学
Journal of Food Science and Technology-mysore Pub Date : 2018-01-01 DOI: 10.3969/j.issn.2095-6002.2018.01.001
陈峰, 孙浩, 倪辉, 洪鹏, 李丽君, 杨远帆, 姜泽东
{"title":"柚子精油提取、成分、贮藏及应用研究进展","authors":"陈峰, 孙浩, 倪辉, 洪鹏, 李丽君, 杨远帆, 姜泽东","doi":"10.3969/j.issn.2095-6002.2018.01.001","DOIUrl":"https://doi.org/10.3969/j.issn.2095-6002.2018.01.001","url":null,"abstract":"","PeriodicalId":16004,"journal":{"name":"Journal of Food Science and Technology-mysore","volume":"8 1","pages":"1-10"},"PeriodicalIF":3.1,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86472416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of Enterococcus faecium Ch-1 isolated from Chuli- A traditional fermented apricot product of Trans Himalayan region 跨喜马拉雅地区传统发酵杏产品Chuli中粪肠球菌Ch-1的分离鉴定
IF 3.1 3区 农林科学
Journal of Food Science and Technology-mysore Pub Date : 2017-08-09 DOI: 10.25177/jfst.2.1.4
J. Bajwa, Anupama Sharma, N. Sharma, Sift Desk Journals Open Access Journals
{"title":"Evaluation of Enterococcus faecium Ch-1 isolated from Chuli- A traditional fermented apricot product of Trans Himalayan region","authors":"J. Bajwa, Anupama Sharma, N. Sharma, Sift Desk Journals Open Access Journals","doi":"10.25177/jfst.2.1.4","DOIUrl":"https://doi.org/10.25177/jfst.2.1.4","url":null,"abstract":"Background: Chuli is a naturally fermented apricot fruit product of Himachal Pradesh and is a rich source of polyphenols and other phytochemicals such as betacarotene and ascorbic acid. In the present research work potential lactic acid bacteria were isolated and explored for its novelty as potential probiotics. Methods: Isolates were screened on the basis of broadest inhibitory spectrum against var ious food borne pathogens i.e , and hence was selected for further study i.e acid and bile tolerance, adherence to gastric mucin sensitivity towards different antibiotics. its relative safety as probiotic candidate was also assessed. Results: Highest antagonism was show by Enterococcus faecium Ch-1 and was found to be tolerant to low pH and high bile concentrations, adherance to gastric mucin. E. faecium Ch-1 was found negative for gelatinase, DNase enzyme activity and haemolysis thus validating its relative safety as probiotic candidate. Conclusion: Enterococcus faecium Ch-1 was found to be a good probiotic strain with cumulative probiotic score of 100% therefore, could be promising for the development as suitable isolate for use in functional foods.","PeriodicalId":16004,"journal":{"name":"Journal of Food Science and Technology-mysore","volume":"28 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2017-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85390725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
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