用转录组分析鉴定芒果叶片和果实中可能的过敏原

IF 2.6 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY
Huiqing Yan, Xiaolong Huang, Zongmin Wu, Yin Yi, Sift Desk Journals Open Access Journals
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引用次数: 2

摘要

以前在芒果(Mangifera indica Linn)中发现了一些过敏原,包括侧链蛋白、betv - 1样蛋白和几丁质酶。在本文中,我们使用高通量Illumina测序对芒果潜在过敏原进行了最深入的研究。RNA-Seq共生成11,751,123个contigs,这些contigs被组装成99,328个unigenes,其中16,848个unigenes >1000 bp。共有230242条unigenes被公共蛋白数据库注释,截止e值大于10,其中27295条、46030条、24227条和14023条unigenes被归属于基因本体术语、Nr、Swiss-Prot和同源基团簇。共鉴定出66个潜在过敏原基因,利用Illumina RNA-Seq技术对其相对表达进行分析。过敏原主要属于花粉过敏原、致病相关蛋白betv I家族和nadph依赖性FMN还原酶。我们选择了c61327。以高表达的graph_c0为模板,在RCSB PDB库(PDB: 4z8w)中获得同源蛋白结构。我们过表达并纯化了c61327。graph_c0,通过免疫印迹可以与人IgE结合
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Putative Allergens Identified in Mango (Mangifera indica Linn) Leaf and Fruit with Transcriptome Analysis
Some allergens have previously been identified in mango (Mangifera indica Linn), including profilins, Bet v 1like proteins and chitinase. In this paper, we identified the deepest investigation of mango potential allergens using high-throughput Illumina sequencing. RNA-Seq generated 11,751,123 contigs that were assembled into 99,328 unigenes with 16,848 unigenes of >1000 bp. A total of 230,242 unigenes were annotated using public protein databases, with a cut-off E-value above 10, of which 27,295, 46,030, 24,227 and 14,023 unigenes were assigned to gene ontology terms, Nr, Swiss-Prot and clusters of orthologous groups, respectively. A total of 66 potential allergen genes were identified, and their relative expressions were evaluated using Illumina RNA-Seq technology. Allergens mainly belonged to pollen allergen, pathogenesis-related protein Bet v I family and NADPH-dependent FMN reductase. We selected c61327.graph_c0, highly expressed in fruit and annotated as Pollen Ole e 1, was used as a template to obtain homologous protein structure in the RCSB PDB bank (PDB: 4z8w). We over-expressed and purified c61327.graph_c0, which could bind to human IgE by immunoblotting
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来源期刊
CiteScore
7.70
自引率
0.00%
发文量
274
审稿时长
11 months
期刊介绍: The Journal of Food Science and Technology (JFST) is the official publication of the Association of Food Scientists and Technologists of India (AFSTI). This monthly publishes peer-reviewed research papers and reviews in all branches of science, technology, packaging and engineering of foods and food products. Special emphasis is given to fundamental and applied research findings that have potential for enhancing product quality, extend shelf life of fresh and processed food products and improve process efficiency. Critical reviews on new perspectives in food handling and processing, innovative and emerging technologies and trends and future research in food products and food industry byproducts are also welcome. The journal also publishes book reviews relevant to all aspects of food science, technology and engineering.
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