Journal of Experimental Pharmacology最新文献

{"title":"Pharmacological Effects of the Lipidosterolic Extract from <i>Kigelia africana</i> Fruits in Experimental Benign Prostatic Hyperplasia Induced by Testosterone in Sprague Dawley Rats.","authors":"Cristina Occhiuto,&nbsp;Giuseppe Santoro,&nbsp;Peter Quinto Tranchida,&nbsp;Giovanni Bono,&nbsp;Francesco Occhiuto","doi":"10.2147/JEP.S383699","DOIUrl":"https://doi.org/10.2147/JEP.S383699","url":null,"abstract":"<p><strong>Background: </strong>The use of phytotherapics is very frequent in men with prostatic diseases, sexual disorders and infertility, and many associations are commercially available. Various vegetable products used as drugs or nutraceuticals are attributed to possess the capacity to exert benefic effects on the reproductive system, and most of these drugs have a rich and varied lipidosterolic fraction, primarily responsible for the effects related to the male genital sphere. <i>Kigelia africana</i> (Lam.) Benth. (Bignoniaceae) is a plant used in African folk medicine as a vegetal remedy for various diseases, including some disorders of the male reproductive system; however, its potential activities have not yet been fully explored. The aim of the present study was to investigate whether the lipidosterolic hexane extract (LHE) from <i>K. africana</i> fruits, analyzed by comprehensive two-dimensional gas chromatography-mass spectrometry/flame ionization detection (GC×GC-MS/FID), can prevent or reverse benign prostatic hyperplasia (BPH) in rats.</p><p><strong>Methods: </strong>BPH was induced in experimental groups by daily subcutaneous injections of testosterone propionate (TP) for four weeks. β-sitosterol (β-s) was used as positive control. On day 28, the animals were sacrificed by cervical dislocation after anesthesia. Prostates were excised, weighed, and used for macroscopic and histological studies. Testosterone and dihydrotestosterone (DHT) levels in prostate were measured.</p><p><strong>Results: </strong>The results showed that LHE significantly reduced the prostatic weight, prostatic index, prostatic levels of testosterone and DHT, and the histopathological alterations (including the epithelial thickness, stromal proliferation, and lumen area) induced by testosterone. These effects were superior to those demonstrated by β-s and appear to be due to a partial antiandrogenic activity of LHE.</p><p><strong>Conclusion: </strong>The results obtained showed that the LHE can prevent, and reverse testosterone induced prostatic hyperplasia, and support the traditional use of <i>Kigelia africana</i> in some disorders of the reproductive system.</p>","PeriodicalId":15846,"journal":{"name":"Journal of Experimental Pharmacology","volume":"15 ","pages":"41-50"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/db/07/jep-15-41.PMC9911901.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10703628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response to Article \"Evaluation of the Anti-Malarial Activity of the Crude Root Extract and Solvent Fraction of <i>Sesamum indicum</i> (Fabaceae)\" [Letter].","authors":"Putri Reno Intan,&nbsp;Ariyani Noviantari","doi":"10.2147/JEP.S418029","DOIUrl":"https://doi.org/10.2147/JEP.S418029","url":null,"abstract":"","PeriodicalId":15846,"journal":{"name":"Journal of Experimental Pharmacology","volume":"15 ","pages":"215-216"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/b0/58/jep-15-215.PMC10164382.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9498255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Aloe adigratana</i> Reynolds: Preliminary Phytochemical Screening, Proximate Content, Essential Oil Analysis, and In Vitro Antifungal Activity Studies of Its Leaf Peels and Gel.","authors":"Abraha Gebremariam,&nbsp;Brhane Gebremedhin Gebrezgabher,&nbsp;Kebede Taye Desta,&nbsp;Desta Berhe Sbhatu,&nbsp;Goitom Gebreyohannes Berhe,&nbsp;Mahmud Abdirkadir,&nbsp;Ephrem Tsegay","doi":"10.2147/JEP.S420990","DOIUrl":"https://doi.org/10.2147/JEP.S420990","url":null,"abstract":"<p><strong>Background: </strong><i>Aloe</i> species are among the most significant plants with several applications. Many of the species, however, are underexplored, owing to their scarcity and limited geographical distribution. <i>A. adigratana</i> Reynolds, which is common in Ethiopia, is one of the little-studied and endangered <i>Aloe</i> species.</p><p><strong>Objective: </strong>This preliminary study focuses on the phytochemical screening, proximate analysis, essential oil content, and antifungal activities of <i>A. adigratana</i> leaf peels. Antifungal activities were also tested on the gels of the plant for comparison.</p><p><strong>Methods: </strong>Standard procedures were used for phytochemical and proximate composition studies. Essential oil analysis was performed using a gas chromatography-mass spectrometry instrument. Using the well-diffusion method, investigations on antifungal activity were performed on three clinically isolated specimens of dandruff-causing fungus; namely, <i>Malassezia furfur, Malassezia restricta</i>, and <i>Malassezia globosa</i>.</p><p><strong>Results: </strong>The leaf peels of <i>A. adigratana</i> contained alkaloids, flavonoids, tannins, and terpenes. The mean moisture, ash, and crude fat levels were 85.69, 92.20, and 8.00%, respectively, whereas the mean total protein and mean total carbohydrate values were 2.59 and 3.04%. Gas chromatography-mass spectrometry investigation confirmed the presence of fifteen essential oils. The most prevalent essential oil component was discovered to be phytol (33.78%), followed by decane (11.29%). In a dose-dependent way, the leaf latex and gel extracts prevented the growth of three dandruff-causing <i>Malassezia</i> fungal species (<i>M. furfur, M. restricta</i>, and <i>M. globosa</i>). Both the latex and gel demonstrated the maximum activity on <i>M. globosa</i>, the most prevalent fungus in the research area, with minimum inhibitory concentrations of 0.24 and 0.48 mg/mL and minimum fungicidal concentrations of 0.48 and 0.97 mg/mL, respectively.</p><p><strong>Conclusion: </strong>In general, the proximate and essential oil compositions of <i>A. adigratana</i> leaves were comparable to those of other <i>Aloe</i> species widely used in the food, cosmetic, and pharmaceutical industries, implying that <i>A. adigratana</i> could be a potential future plant for such industries.</p>","PeriodicalId":15846,"journal":{"name":"Journal of Experimental Pharmacology","volume":"15 ","pages":"321-332"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/eb/cf/jep-15-321.PMC10473414.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10155748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response to Article \"Antipyretic Potential of 80% Methanol Extract and Solvent Fractions of <i>Bersama abyssinica</i> Fresen. (Melianthaceae) Leaves Against Yeast-Induced Pyrexia in Mice\" [Response to Letter].","authors":"Bantayehu Addis Tegegne,&nbsp;Agumas Alemu Alehegn","doi":"10.2147/JEP.S414817","DOIUrl":"https://doi.org/10.2147/JEP.S414817","url":null,"abstract":"","PeriodicalId":15846,"journal":{"name":"Journal of Experimental Pharmacology","volume":"15 ","pages":"189-190"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/fe/d3/jep-15-189.PMC10106797.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9383974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy and Anti-Inflammatory Activity of Ashwagandha Sustained-Release Formulation on Depression and Anxiety Induced by Chronic Unpredictable Stress: in vivo and in vitro Studies.","authors":"Alluri Venkata KrishnaRaju,&nbsp;Venkateswarlu Somepalli,&nbsp;Shefali Thanawala,&nbsp;Rajat Shah","doi":"10.2147/JEP.S407906","DOIUrl":"https://doi.org/10.2147/JEP.S407906","url":null,"abstract":"<p><strong>Background: </strong>Stress is the psychological, physiological, and behavioral response of an individual's body when they perceive a lack of equilibrium between the demands placed upon them and their ability to meet those demands. Adaptogens are herbs that help with stress management, and Ashwagandha is one such safe and effective adaptogen.</p><p><strong>Objective: </strong>We evaluated the anti-neuroinflammatory potential of Ashwagandha sustained-release formulation (AshwaSR) by estimating the in vitro expression of pro-inflammatory cytokines, and its efficacy on anxiety and depression in an in vivo study.</p><p><strong>Methods: </strong>Our in vitro study investigated the anti-inflammatory potential of AshwaSR by estimating the expression of tumour necrosis factor [TNF]-α and interleukin [IL]-1β levels in LPS-induced THP-1 human monocytes, and the antioxidant effects by its potential to inhibit the superoxide [SO] generation in PMA-induced HL-60 human monocytic cells. The in vivo study assessed the efficacy of AshwaSR on chronic unpredictable stress (CUS)-induced comorbid anxiety and depression in <i>Sprague Dawley</i> rats. Antidepressant and anxiolytic effects of AshwaSR were evaluated by open field test (OFT), elevated plus maze (EPM), forced swim test (FST), and Morris water maze (MWM) test.</p><p><strong>Results: </strong>AshwaSR inhibited TNF-α, IL-1β and superoxide production in a dose-dependent manner in the in vitro study. The in vivo CUS model induced depression-like and anxiety-like behaviour. Treatments with AshwaSR and escitalopram showed improvement in the EPM and MWM models compared to the CUS-group.</p><p><strong>Conclusion: </strong>In vitro study demonstrated that AshwaSR inhibits expressions of pro-inflammatory cytokines, IL-1β and TNF-α, and superoxide production. Further, the in vivo study confirmed its anxiolytic and stress-relieving effects in the CUS model that confirmed AshwaSR's potential in managing stress and stress-related symptoms.</p>","PeriodicalId":15846,"journal":{"name":"Journal of Experimental Pharmacology","volume":"15 ","pages":"291-305"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/8e/96/jep-15-291.PMC10386834.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9919708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum: Pharmacological Activities of Fingerroot Extract and Its Phytoconstituents Against SARS-CoV-2 Infection in Golden Syrian Hamsters [Corrigendum].","authors":"","doi":"10.2147/JEP.S411939","DOIUrl":"https://doi.org/10.2147/JEP.S411939","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.2147/JEP.S382895.].</p>","PeriodicalId":15846,"journal":{"name":"Journal of Experimental Pharmacology","volume":"15 ","pages":"137-138"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c0/8a/jep-15-137.PMC10024502.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9146807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Systematic Review of Ethnobotanical, Phytochemical, and Ethnopharmacological Studies of <i>Urtica simensis</i> (Stinging Nettle).","authors":"Tesfaye Yimer Tadesse,&nbsp;Mulugeta Molla Zeleke,&nbsp;Samuel Berihun Dagnew,&nbsp;Getu Tesfaw Addis","doi":"10.2147/JEP.S404506","DOIUrl":"https://doi.org/10.2147/JEP.S404506","url":null,"abstract":"<p><p>The Urticaceae family contains 54 genera and more than 2000 species that can be found in tropical, subtropical, and temperate climates all over the world. This family includes the largest genus in the world, <i>Urtica</i>, which is also known as stinging nettle. Stinging hairs are present on the lower surface of the leaves and beneath the stems of <i>Urtica simensis</i>, also known as the stinging nettle, herbal nettle that is dioecious, upright, and unbranched. For the treatment of conditions like gastritis, heart disease, diabetes, gonorrhea, and malaria, people employ various portions of <i>Urtica simensis</i> in a variety of ways in traditional medicine. The <i>Urtica simensis</i> leaves are rich in variety of active secondary phytochemical constituents including terpenoids, saponins, tannins, flavonoids, steroids, alkaloids, polyphenols, sterols, oxalate, and ascorbic acid (vitamin C). According to different reports, it possesses a variety of pharmacological properties, including antioxidant, antiproliferative, antidiabetic, cardioprotective, antiulcer, antibacterial, and antifungal actions. The current review summarizes published and unpublished information about the ethnobotanical, phytochemical, ethnopharmacological, and toxicological reports of <i>Urtica simensis</i> and summarizes all the research work carried out on this plant to provide updated information for future work.</p>","PeriodicalId":15846,"journal":{"name":"Journal of Experimental Pharmacology","volume":"15 ","pages":"177-186"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/30/a1/jep-15-177.PMC10075218.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9266674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antipyretic Potential of 80% Methanol Extract and Solvent Fractions of <i>Bersama abyssinica</i> Fresen. (Melianthaceae) Leaves Against Yeast-Induced Pyrexia in Mice.","authors":"Bantayehu Addis Tegegne,&nbsp;Agumas Alemu Alehegn","doi":"10.2147/JEP.S390825","DOIUrl":"https://doi.org/10.2147/JEP.S390825","url":null,"abstract":"<p><strong>Introduction: </strong>Since fever is a complicated physiological reaction to an infection or aseptic stimulus, finding safer solutions that are more potent and derived from plants is essential to resolving this issue. <i>Bersama abyssinica</i> (Melianthaceae) is traditionally used to treat fever, though this has yet to be proven scientifically.</p><p><strong>Objective: </strong>The present study aimed to assess the antipyretic potential of leaf extract and solvent fractions of <i>B. abyssinica</i>.</p><p><strong>Methods: </strong>The antipyretic activities of crude extract and solvent fractions of <i>B. abyssinica</i> leaves were evaluated using a yeast-induced pyrexia model at three different dose ranges (100mg/kg, 200mg/kg, and 400mg/kg) methanol extract as well as chloroform, ethyl acetate, and aqueous fractions to mice showing an increase in temperature of ≥0.5 °C. The rectal temperature of each mouse was recorded using a digital thermometer. To analyze the data, SPSS version 20 and one-way ANOVA followed by Tukey's HSD post hoc test to compare results between groups were utilized.</p><p><strong>Results: </strong>The crude extract demonstrated significant antipyretic potential (P<0.05 by 100 mg/kg and 200 mg/kg as well as P<0.01 by 400 mg/kg), with a maximum of 95.06% reduction in rectal temperature at 400 mg/kg, comparable to 98.37% at 2.5 hours by the standard drug. Similarly, all doses of the aqueous fraction, as well as 200 mg/kg and 400 mg/kg doses of the ethyl acetate fractions, resulted in a significant (P<0.05) reduction in rectal temperature when compared to the corresponding value of the negative control group.</p><p><strong>Conclusion: </strong>Extracts of <i>B. abyssinica</i> leaves were found to have a significant antipyretic effect. Thus, the use of the plant for pyrexia in traditional settings has scientific ground.</p>","PeriodicalId":15846,"journal":{"name":"Journal of Experimental Pharmacology","volume":"15 ","pages":"81-91"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/df/59/jep-15-81.PMC9985388.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10857225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pomegranate Seeds and Peel Ethanolic Extracts Anticancer Potentials and Related Genetic, Histological, Immunohistochemical, Apoptotic and Oxidative Stress Profiles: In vitro Study.","authors":"Mohamed Nasr,&nbsp;Suhaib Alsayed Naeem,&nbsp;Ibrahim El-Shenbaby,&nbsp;Fatma Mahmoud Abdelraheem Mohamed,&nbsp;Safinaz Moustafa Mahmoud,&nbsp;Tamer M M Abuamara,&nbsp;Wagih M Abd-Elhay,&nbsp;Fayez Mohammed Abd Elfattah Elbayoumy,&nbsp;Ahmad Elkot,&nbsp;Tarek Shikhon,&nbsp;Mostafa Abo-Akrab,&nbsp;Mohamed A Doma,&nbsp;Abdulkarim Hasan","doi":"10.2147/JEP.S404321","DOIUrl":"https://doi.org/10.2147/JEP.S404321","url":null,"abstract":"<p><strong>Introduction: </strong>Owing to their great quantity of hydrolyzable anthocyanins and tannins, the peel and seeds of pomegranate are edible and possess potent anti-oxidant and anti-inflammatory characteristics. This work aims to trace the pomegranate seed and peel ethanolic extracts' anticancer activity against liver cancer cell line, namely HepG2 and related histopathological, immunohistochemical, genetic and oxidative stress profile.</p><p><strong>Methods: </strong>In vitro study for both seed and peel extract showed the prevalence of phenols, polyphenols and acids, those have anti-proliferative potential against liver cancer cell line (HepG2) with 50% inhibitory concentration (IC50) of seed significantly reduced that of peel. Toxicity of test extracts was concentration dependent and accompanied with cell cycle arrest and cell death at theG0/G1 and S phases but not at the G2/M phase. Cell arrest was supplemented with raised ROS, MDA and decreased SOD, GSH and Catalase.</p><p><strong>Results and discussion: </strong>Apoptosis-related genes showed significant up-expression of pro-apoptotic gene (<i>P53</i>), <i>Cy-C</i>, <i>Bax</i>, and <i>casp-3</i> and down expression of anti-apoptotic gene (<i>Bcl-2</i>). Also, Casp-3 and P53 proteins were substantially expressed under the effect of test extracts. Histopathological study demonstrated that the untreated cells (control group) were regular cells with nuclear pleomorphism and hyperchromatic nuclei, while seed and peel extracts-treated cells showed necrosis, mixed euchromatin and heterochromatin, intra-nuclear eosinophilic structures, burst cell membranes, and the shrunken apoptotic cells with nuclear membranes and irregular cells. Finally, <i>PCNA</i> gene detected by immunohistochemistry was down regulated significantly under the effect of seed extract treatment than in case of cell medication with peel extract.</p>","PeriodicalId":15846,"journal":{"name":"Journal of Experimental Pharmacology","volume":"15 ","pages":"191-205"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/04/fb/jep-15-191.PMC10115208.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9387206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Analgesics and Anti-Inflammatory Activity of the Root Extract of <i>Impatiens rothii (Balsaminaceae)</i> in Rodents.","authors":"Getachew Ashagrie,&nbsp;Fentaw Girmaw,&nbsp;Abebe Tarekegn,&nbsp;Tenaw Baye,&nbsp;Abebe Dagne","doi":"10.2147/JEP.S410024","DOIUrl":"https://doi.org/10.2147/JEP.S410024","url":null,"abstract":"<p><strong>Background: </strong>The roots of <i>Impatiens rothii</i> has been used as a traditional remedy for painful conditions, rheumatism, isthmus and crural aches. However, the analgesic and anti-inflammatory properties of this plant have yet to be scientifically confirmed. The purpose of this study was to explore possible analgesic and anti-inflammatory activities 80% methanolic root extract of <i>Impatiens rothii</i>.</p><p><strong>Methods: </strong>To obtain the crude extract, the roots of <i>Impatiens rothii</i> that had been dried and ground up were macerated in 80% methanol. The analgesic activity was determined using acetic acid-induced writhing and hot plate tests in mice, whereas the anti-inflammatory activity was analyzed using carrageenan-induced paw edema model in rats. The extract was orally administered at a dose of 100, 200 and 400 mg/kg.</p><p><strong>Results: </strong>All tested doses of <i>Impatiens rothii</i> extract showed significant analgesic activity (p<0.05) at observations of 30 to 120 minutes compared to the negative control in the hot plate test. In acetic acid-induced writhing test all tested doses of the 80% methanol extract of <i>Impatiens rothii</i> significantly (p < 0.001) reduced the number of writhing. In comparison to the control group, all tested doses displayed a significant decrease in paw edema, which appeared 2-5 hours after induction (p<0.05).</p><p><strong>Conclusion: </strong>From the results of this study, it can be stated that 80% methanolic extract of <i>Impatiens rothii</i> possessed substantial analgesic and anti-inflammatory activities, hence providing scientific basis for the use of this plant in the treatment of pain and inflammatory diseases.</p>","PeriodicalId":15846,"journal":{"name":"Journal of Experimental Pharmacology","volume":"15 ","pages":"207-214"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/38/ed/jep-15-207.PMC10124745.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9413770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}