Journal of General Plant Pathology最新文献_第2页

First report of poor growth and root rot caused by Phytopythium helicoides on shiso plants in a deep flow technique hydroponic system 首次报告紫苏植物在深流技术水培系统中生长不良和根部腐烂的情况

IF 1.2 4区农林科学

Journal of General Plant Pathology Pub Date : 2024-05-31 DOI: 10.1007/s10327-024-01183-3

Noriyuki Miyake, Masashi Matsusaki, Hiroko Yasuda, Yoshimasa Kubota, Takamasa Otake, Ayaka Hieno, Koji Kageyama

引用次数: 0

Multiplex PCR specific for genus Phytophthora and P. nicotianae with an internal plant DNA control for effective quarantine of Phytophthora species in Japan 特异于 Phytophthora 和 P. nicotianae 的多重 PCR 与植物内部 DNA 对照，用于日本 Phytophthora 物种的有效检疫

IF 1.2 4区农林科学

Journal of General Plant Pathology Pub Date : 2024-05-13 DOI: 10.1007/s10327-024-01179-z

Kayoko Otsubo, Mingzhu Li, Auliana Afandi, Haruhisa Suga, Koji Kageyama, Ayaka Hieno

{"title":"Multiplex PCR specific for genus Phytophthora and P. nicotianae with an internal plant DNA control for effective quarantine of Phytophthora species in Japan","authors":"Kayoko Otsubo, Mingzhu Li, Auliana Afandi, Haruhisa Suga, Koji Kageyama, Ayaka Hieno","doi":"10.1007/s10327-024-01179-z","DOIUrl":"https://doi.org/10.1007/s10327-024-01179-z","url":null,"abstract":"To prevent threats from pathogens such as Phytophthora species from international plant trade, molecular identification techniques are needed for rapid, accurate quarantine inspection. Here, for quarantine control in Japan, we developed a simple DNA extraction for plants and a practical detection method that combines multiplexed PCR using primers specific for Phytophthora species, for P. nicotianae, which is the only non-quarantine Phytophthora species, and as internal controls, for plants. For the new genus-level primer set, we modified previously reported genus-specific primers to improve detectability. The new primers were able to detect mycelial DNA of 155 taxa among Phytophthora clades 1–10, with a sensitivity of 100 fg/µL for three representative species, P. ramorum, P. kernoviae and P. nicotianae. In the PCRs using DNA from non-target species, amplification was observed for only three taxa, and for some strains, four taxa in a closely related genus. Duplex and triplex PCR of the genus-specific primers combined with previously reported plant primers verified the success of DNA extraction and PCR detection from diseased plant samples, and in the triplex PCR, whether the pathogen was diagnosed as P. nicotianae or not by the species-specific primer. The new method detected the pathogen in naturally infected and inoculated plants. The amplicons using the genus-specific primer have enough variation to be sequenced to identify the species. This new method can be used immediately for detecting Phytophthora species and for quarantine control in Japan.","PeriodicalId":15825,"journal":{"name":"Journal of General Plant Pathology","volume":"42 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140930181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization of Japanese isolates of soil-borne wheat mosaic virus in the genus Furovirus 日本土传小麦花叶病毒属分离物的分子特征

IF 1.2 4区农林科学

Journal of General Plant Pathology Pub Date : 2024-05-06 DOI: 10.1007/s10327-024-01177-1

Takehiro Ohki, Hitoshi Matsunaka

引用次数: 0

The first report of rot disease of onion occurred by Pseudomonas kitaguniensis, causing severe rot symptoms at lower temperatures 首次报告的洋葱腐烂病是由北国假单胞菌（Pseudomonas kitaguniensis）引起的，在较低温度下会导致严重的腐烂症状

IF 1.2 4区农林科学

Journal of General Plant Pathology Pub Date : 2024-04-27 DOI: 10.1007/s10327-024-01178-0

Mizue Tsuji, Moe Sato, Shin-ichi Fuji

引用次数: 0

Isolation of spontaneous mutants of tomato brown rugose fruit virus that efficiently infect Tm-1 homozygote tomato plants 分离出能有效感染 Tm-1 基因型番茄植株的番茄褐皱果病毒自发突变体

IF 1.2 4区农林科学

Journal of General Plant Pathology Pub Date : 2024-04-17 DOI: 10.1007/s10327-024-01176-2

Kenji Kubota, Sawana Takeyama, Yosuke Matsushita, Kazuhiro Ishibashi

{"title":"Isolation of spontaneous mutants of tomato brown rugose fruit virus that efficiently infect Tm-1 homozygote tomato plants","authors":"Kenji Kubota, Sawana Takeyama, Yosuke Matsushita, Kazuhiro Ishibashi","doi":"10.1007/s10327-024-01176-2","DOIUrl":"https://doi.org/10.1007/s10327-024-01176-2","url":null,"abstract":"The tobamovirus, tomato brown rugose fruit virus (ToBRFV), is a significant concern in global tomato production due to the ineffectiveness of the widely used Tm-22 resistance gene. Our previous study showed that the tomato variety GCR237, a Tm-1 homozygote, resisted an Israeli isolate of ToBRFV (DSMZ PV-1241) for up to 35 days post inoculation (dpi), suggesting Tm-1-homozygous cultivars could control ToBRFV. In the present study, we inoculated GCR237 plants with ToBRFV and cultivated them for a longer period of time. The plants resisted systemic infection up to 50 dpi, but mosaic symptoms appeared on the upper leaves by 100 dpi. We retrieved the virus from symptomatic leaves and established four single local lesion isolates. These isolates had several amino acid (AA) substitutions in the helicase domain of 126-kDa/183-kDa replication proteins, where the Tm-1 protein likely binds to inhibit viral RNA replication. Back-inoculating these isolates onto GCR237 plants confirmed they had acquired the ability to overcome GCR237’s resistance and induced mosaic symptoms as early as 14 dpi. About 90% of 229 ToBRFV isolates in the NCBI database had identical AA sequences in the corresponding region to DSMZ PV-1241, while ~ 10% inherently had AA substitutions that would confer complete breaking ability to the Tm-1 resistance. These results suggest that while Tm-1 can inhibit ToBRFV RNA replication, ToBRFV can easily overcome Tm-1 homozygotes.","PeriodicalId":15825,"journal":{"name":"Journal of General Plant Pathology","volume":"5 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140615362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First report of fludioxonil resistance isolate of Colletotrichum fructicola emerging on strawberry in Japan 首次报告日本草莓上出现的对氟啶虫酰胺有抗性的果孢子菌分离株

IF 1.2 4区农林科学

Journal of General Plant Pathology Pub Date : 2024-04-16 DOI: 10.1007/s10327-024-01174-4

Akiko Furuta, Yoichi Ide, Nobuya Tashiro, Motoaki Kusaba

{"title":"First report of fludioxonil resistance isolate of Colletotrichum fructicola emerging on strawberry in Japan","authors":"Akiko Furuta, Yoichi Ide, Nobuya Tashiro, Motoaki Kusaba","doi":"10.1007/s10327-024-01174-4","DOIUrl":"https://doi.org/10.1007/s10327-024-01174-4","url":null,"abstract":"The species composition of the strawberry anthracnose pathogen population in Saga Prefecture was investigated through phylogenetic analysis of the nucleotide sequences of the intergenic regions of the apn2 and MAT1-2–1 genes. Here, 77 out of the 80 Colletotrichum isolates used were classified as C. fructicola, and the remaining three were classified as C. siamense. The 77 C. fructicola isolates were tested for sensitivity to fludioxonil, which is widely used as the main fungicide in rotational programs in Saga Prefecture. High levels of resistance to fludioxonil were observed in 14 C. fructicola isolates tested for sensitivity to mycelial growth in fludioxonil-containing media. Furthermore, two inoculation assays using strawberry seedlings treated with fludioxonil were performed on five of the 14 isolates that showed high levels of resistance. The effectiveness of fludioxonil was significantly low in these isolates. To our knowledge, this is the first study to report fludioxonil resistance in a C. fructicola population.","PeriodicalId":15825,"journal":{"name":"Journal of General Plant Pathology","volume":"1 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140565957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Camalexin quantification using HPLC and the novel internal standard thiabendazole 利用高效液相色谱法和新型内标噻苯咪唑定量茶苯海明

IF 1.2 4区农林科学

Journal of General Plant Pathology Pub Date : 2024-04-12 DOI: 10.1007/s10327-024-01175-3

Dominik K. Großkinsky, Stefanie M. Primisser, Thomas Reichenauer, Hartwig Pfeifhofer

引用次数: 0

Flagellar motility of Pseudomonas syringae pv. actinidiae biovar 3 contributes to bacterial infection through stomata 丁香假单胞菌 pv. actinidiae 生物变种 3 的鞭毛运动有助于细菌通过气孔进行感染

IF 1.2 4区农林科学

Journal of General Plant Pathology Pub Date : 2024-04-10 DOI: 10.1007/s10327-024-01172-6

Giyu Usuki, Takako Ishiga, Nanami Sakata, Yasuhiro Ishiga

{"title":"Flagellar motility of Pseudomonas syringae pv. actinidiae biovar 3 contributes to bacterial infection through stomata","authors":"Giyu Usuki, Takako Ishiga, Nanami Sakata, Yasuhiro Ishiga","doi":"10.1007/s10327-024-01172-6","DOIUrl":"https://doi.org/10.1007/s10327-024-01172-6","url":null,"abstract":"Highly virulent Pseudomonas syringae pv. actinidiae biovar 3 (Psa3) causes kiwifruit bacterial canker, which seriously damages kiwifruits worldwide. We previously screened Psa3-transposon-inserted mutants and obtained reduced-virulence mutants with genes encoding the flagellar protein. Flagella are involved in the motility of bacteria and contribute to P. syringae invasion of plant leaves. However, whether flagella are required for the ability to enter stomata and then multiply in the plant apoplast is not known. Here, we sprayed kiwifruit leaves with Psa3-flagellar-defective mutants and found that their virulence was reduced compared to that of the Psa3 wild type, but their virulence was not reduced when the leaves were infiltrated with Psa3-flagellar-defective mutants using a syringe. Motility and bacterial entry into apoplastic space of the flagellar-defective mutants were also significantly reduced. These results indicate that flagella contribute to Psa3 motility and stomata-mediated entry, leading to disease development, but not multiplication in apoplast space after Psa3 entry. This study provides new insight into bacterial motility in host plants.","PeriodicalId":15825,"journal":{"name":"Journal of General Plant Pathology","volume":"52 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140565847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First report of Pythium aphanidermatum causing damping off, collar and root rot of coriander in Brazil 首次报告 Pythium aphanidermatum 在巴西导致芫荽受潮、领腐和根腐病

IF 1.2 4区农林科学

Journal of General Plant Pathology Pub Date : 2024-03-04 DOI: 10.1007/s10327-024-01171-7

Eveline Mendes da Silva, Natália Deniz Brito, Naasoom Luiz Santos Mesquita, Poliana Prates de Souza Soares, Rafael José Vilela de Oliveira, Armínio Santos, Quelmo Silva de Novaes

引用次数: 0

First report of potato spindle tuber viroid isolated from pepper seeds produced in Vietnam 首次报告从越南生产的辣椒种子中分离出马铃薯纺锤形块茎病毒

IF 1.2 4区农林科学

Journal of General Plant Pathology Pub Date : 2024-02-15 DOI: 10.1007/s10327-024-01170-8

引用次数: 0