Journal of General Plant Pathology最新文献

Development of a real-time loop-mediated isothermal amplification method with toothpick sampling for non-destructive detection of Ustilago esculenta in Zizania latifolia 开发一种实时环介导等温扩增法和牙签取样法，用于无损检测菰中的 Ustilago esculenta

IF 1.2 4区农林科学

Journal of General Plant Pathology Pub Date : 2024-09-17 DOI: 10.1007/s10327-024-01201-4

Ryunosuke Yamada, Nobumitsu Sasaki, Ken Komatsu, Kouji Mashimo, Takashi Motobayashi

{"title":"Development of a real-time loop-mediated isothermal amplification method with toothpick sampling for non-destructive detection of Ustilago esculenta in Zizania latifolia","authors":"Ryunosuke Yamada, Nobumitsu Sasaki, Ken Komatsu, Kouji Mashimo, Takashi Motobayashi","doi":"10.1007/s10327-024-01201-4","DOIUrl":"https://doi.org/10.1007/s10327-024-01201-4","url":null,"abstract":"Infection of Zizania latifolia by the basidiomycete Ustilago esculenta enlarges the stem tissue to produce edible galls called \"makomotake\" in Japan. The development of stem galls in Z. latifolia may be influenced by the colonization level of U. esculenta at the seedling stage. To evaluate this possibility, it is necessary to establish a method to investigate the relationship between fungal levels in seedlings and the production of makomotake from the same seedlings in a non-destructive manner. To achieve this goal, in this study, we attempted to develop a real-time loop-mediated isothermal amplification (LAMP) method with toothpick sampling for U. esculenta detection. An U. esculenta gene-specific LAMP primer set designed for real-time LAMP showed sufficient detection sensitivity for fungal genomic DNA, which is only about 10 times lower than that of quantitative PCR. Real-time LAMP reactions following toothpick sampling detected U. esculenta efficiently in the culm part of Z. latifolia seedlings that eventually produced makomotake, but did not detect in those that failed to develop stem galls. Our data show that the combination of real-time LAMP and toothpick sampling is a simple and useful method for estimating U. esculenta levels in Z. latifolia seedlings and for studying the subsequent development of stem galls in the same individuals.","PeriodicalId":15825,"journal":{"name":"Journal of General Plant Pathology","volume":"24 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142262898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polymorphic microsatellite markers for genetic analysis of Berkeleyomyces rouxiae, a causal agent of black root rot 多态微卫星标记用于黑根腐病病原菌 Berkeleyomyces rouxiae 的遗传分析

IF 1.2 4区农林科学

Journal of General Plant Pathology Pub Date : 2024-09-06 DOI: 10.1007/s10327-024-01198-w

Akari Oya, Toshiyuki Usami

引用次数: 0

WRKY transcription factors identified in the transcriptome of Citrus latifolia Tan. and their expression in response to Huanglongbing disease 在花叶柑转录组中发现的 WRKY 转录因子及其在黄龙病中的表达

IF 1.2 4区农林科学

Journal of General Plant Pathology Pub Date : 2024-08-20 DOI: 10.1007/s10327-024-01196-y

Berenice Preza-Murrieta, Juan Carlos Noa-Carrazana, Norma Flores-Estévez, Humberto Estrella-Maldonado, Ricardo Santillán-Mendoza, Cristian Matilde-Hernández, Nelly Abigail González-Oviedo, Liliana Eunice Saucedo-Picazo, Felipe Roberto Flores-de la Rosa

{"title":"WRKY transcription factors identified in the transcriptome of Citrus latifolia Tan. and their expression in response to Huanglongbing disease","authors":"Berenice Preza-Murrieta, Juan Carlos Noa-Carrazana, Norma Flores-Estévez, Humberto Estrella-Maldonado, Ricardo Santillán-Mendoza, Cristian Matilde-Hernández, Nelly Abigail González-Oviedo, Liliana Eunice Saucedo-Picazo, Felipe Roberto Flores-de la Rosa","doi":"10.1007/s10327-024-01196-y","DOIUrl":"https://doi.org/10.1007/s10327-024-01196-y","url":null,"abstract":"Huanglongbing (HLB) is the most devastating disease of citrus. Although no citrus species are known to be immune to HLB, some (e.g., Persian lime [Citrus latifolia]) have a high level of tolerance. Differentially expressed genes in the WRKY transcription factor (TF) family have been identified in another tolerant citrus, suggesting an association with such tolerance. Therefore, here we searched for the conserved WRKY domain of WRKY family members in the transcriptome of C. latifolia infected with HLB, characterized the identified ClWRKY genes, and determined which were differentially expressed in leaves with HLB symptoms; 177 transcripts with the WRKY domain were identified, and 32 ClWRKY genes were characterized for conserved motifs, gene ontology, and coexpression networks among the 32 ClWRKY genes. These genes were thus shown to be involved in response to external stimuli and biotic and abiotic stresses. Four differentially expressed ClWRKY genes (ClWRKY20, 23, 47, and 65) were identified in the transcriptome and validated by qRT-PCR. The possible roles of these genes in the tolerance of C. latifolia to HLB are discussed based on the function of homologs of these genes in other plant species. These WRKY genes could contribute to the genetic improvement of susceptible citrus to manage HLB.","PeriodicalId":15825,"journal":{"name":"Journal of General Plant Pathology","volume":"61 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142193621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Possible roles of immunity-related response in modulating chlorosis induced by the silencing of chloroplast HSP90C in tobacco models 在烟草模型中，免疫相关反应在调节叶绿体 HSP90C 沉默诱导的萎黄病中可能发挥的作用

IF 1.2 4区农林科学

Journal of General Plant Pathology Pub Date : 2024-07-22 DOI: 10.1007/s10327-024-01191-3

Okon Odiong Unung, Houssam Eddine Said Bensedira, Takakazu Matsuura, Izumi C. Mori, Yuta Shimomura, Takashi Yaeno, Hidetaka Kaya, Kappei Kobayashi

{"title":"Possible roles of immunity-related response in modulating chlorosis induced by the silencing of chloroplast HSP90C in tobacco models","authors":"Okon Odiong Unung, Houssam Eddine Said Bensedira, Takakazu Matsuura, Izumi C. Mori, Yuta Shimomura, Takashi Yaeno, Hidetaka Kaya, Kappei Kobayashi","doi":"10.1007/s10327-024-01191-3","DOIUrl":"https://doi.org/10.1007/s10327-024-01191-3","url":null,"abstract":"In the inducible chlorosis model tobacco, i-hpHSP90C, the silencing of HSP90C activated both salicylic acid (SA)- and cell death-related gene expression and sporadic cell death, resulting in severe chlorosis. In this model plant, we found a transient SA accumulation to a significantly high level at 8 h after induction of HSP90C silencing and consistent upregulation of CBP60-type transcription factors and some SA biosynthetic genes. Exogenous treatment of the model plant with SA alone did not induce chlorosis. The introgression of a gene encoding SA-degrading enzyme, nahGA430V, into tobacco plants with functional N′ tobamovirus resistance gene partially compromised their resistance to tomato mosaic virus but without a clear reduction in SA levels. Expression of nahGA430V stochastically alleviated chlorosis and, subsequently, sporadic cell death upon induction of HSP90C silencing. We applied tenoxicam, a potent inhibitor of the NPR1-dependent SA signaling pathway in Arabidopsis, and found that it alleviated chlorosis in i-hpHSP90C, which accompanied a reduced expression of a CBP60-type transcription factor. However, the expression of PR1a, a well-characterized SA signal marker, was not suppressed by tenoxicam in the i-hpHSP90 plants with alleviated chlorosis. The findings collectively suggest that the plant immunity-related response, including SA production, could have a role in increasing the severity of chlorosis, although the underlying mechanisms remain to be elucidated.","PeriodicalId":15825,"journal":{"name":"Journal of General Plant Pathology","volume":"7 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141742941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CRISPR/Cas9-mediated resurrection of tobacco NB-LRR class virus resistance gene from a susceptible allele with partial duplication CRISPR/Cas9 介导的烟草 NB-LRR 类病毒抗性基因从部分重复的易感等位基因中复活

IF 1.2 4区农林科学

Journal of General Plant Pathology Pub Date : 2024-07-22 DOI: 10.1007/s10327-024-01189-x

Saki Miyoshi, Okon Odiong Unung, Hidetaka Kaya, Takashi Yaeno, Kappei Kobayashi

{"title":"CRISPR/Cas9-mediated resurrection of tobacco NB-LRR class virus resistance gene from a susceptible allele with partial duplication","authors":"Saki Miyoshi, Okon Odiong Unung, Hidetaka Kaya, Takashi Yaeno, Kappei Kobayashi","doi":"10.1007/s10327-024-01189-x","DOIUrl":"https://doi.org/10.1007/s10327-024-01189-x","url":null,"abstract":"It is still difficult to manipulate the nucleotide-binding site-leucine-rich repeat (NB-LRR) class disease resistance genes because of their large multigenic family. Here, we report the successful application of CRISPR/Cas9 to resurrect a functional allele from a susceptible allele of the N′ tobamovirus resistance gene. The susceptible alleles of N′ from some Nicotiana tabacum cultivars (Nt-n′) have a partial duplication of the N′ coding sequence upstream of a complete coding sequence of the N′ gene, which likely abolished the N′-mediated resistance. We first established a transgenic tobacco line expressing Cas9 under the control of a chemically inducible promoter. The plant line was retransformed with a construct expressing a guide RNA targeting the sequences common to the duplicated partial sequence in the upstream and the complete sequence in the downstream. The T0 transformants had different ratios of the sequences devoid of the duplicated partial sequence. Sequencing proved that some of them had sequences identical to that of the functional N′ gene, suggesting the successful resurrection of the functional N′ gene. The resurrected allele, N′-R, was inherited by a few T1 progenies and subsequent generations with the least mutation at the target site under Cas9-uninduced conditions. The plants homozygous for N′-R showed resistance to a tobamovirus, indicating that the resurrected N′-R allele is functional.","PeriodicalId":15825,"journal":{"name":"Journal of General Plant Pathology","volume":"21 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141776177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lasiodiplodia species isolated from Theobroma cacao in the Philippines and their pathogenicity 从菲律宾可可豆中分离出的 Lasiodiplodia 菌种及其致病性

IF 1.2 4区农林科学

Journal of General Plant Pathology Pub Date : 2024-07-09 DOI: 10.1007/s10327-024-01187-z

Celynne Ocampo-Padilla, Mike Andre C. Malonzo, Rose Ann F. Grospe, John Troy A. Bagsic, Shunsuke Nozawa, Yasuhisa Tsurumi, Kyoko Watanabe

{"title":"Lasiodiplodia species isolated from Theobroma cacao in the Philippines and their pathogenicity","authors":"Celynne Ocampo-Padilla, Mike Andre C. Malonzo, Rose Ann F. Grospe, John Troy A. Bagsic, Shunsuke Nozawa, Yasuhisa Tsurumi, Kyoko Watanabe","doi":"10.1007/s10327-024-01187-z","DOIUrl":"https://doi.org/10.1007/s10327-024-01187-z","url":null,"abstract":"Theobroma cacao is primarily cultivated for its seeds. In the Philippines, where cacao yields are decreased by pod rots and vascular streak dieback (VSD)-like symptoms, the fungus Lasiodiplodia theobromae (Botryospaheriaceae) is the only reported species of Lasiodiplodia reported to cause disease in cacao. Here to identify and determine the pathogenicity of Lasiodiplodia isolates from cacao trees, we isolated Lasiodiplodia from from a leaf with blight (isolate PH22-080), stem with vascular streak (PH22-060), pod rot (PH22-120), an asymptomatic stem (PH22-014), and leaf litter (PH22-007). All strains were pathogenic on wounded leaves and stems and infected pods even without wounds. On the basis of molecular analysis of combined sequence data for ITS, rpb2, tef1-α and tub2 and morphological characteristics, the strains were identified as L. pseudotheobromae (PH22-080), L. theobromae (PH22-120), and Lasiodiplodia sp. (PH22-060), which were confirmed to be pathogenic on cacao leaves, stems, and pods. L. theobromae (PH22-007) and L. hormozganensis (PH22-014) showed potential to cause disease in cacao but further investigation is needed.","PeriodicalId":15825,"journal":{"name":"Journal of General Plant Pathology","volume":"38 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141573887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Variations in mycelial growth and virulence below 26 °C among five Colletotrichum strains from strawberry 草莓中五种 Colletotrichum 菌株在 26 °C 以下菌丝生长和毒力的变化

IF 1.2 4区农林科学

Journal of General Plant Pathology Pub Date : 2024-07-08 DOI: 10.1007/s10327-024-01186-0

Xue Li, Chunnu Geng, Xianyao Huang, Siyu Chen, Jing Yang, Yongchao Han, Fangyan Lu, Ke Duan, Qinghua Gao

{"title":"Variations in mycelial growth and virulence below 26 °C among five Colletotrichum strains from strawberry","authors":"Xue Li, Chunnu Geng, Xianyao Huang, Siyu Chen, Jing Yang, Yongchao Han, Fangyan Lu, Ke Duan, Qinghua Gao","doi":"10.1007/s10327-024-01186-0","DOIUrl":"https://doi.org/10.1007/s10327-024-01186-0","url":null,"abstract":"Anthracnose caused by Colletotrichum spp. is a serious threat to many crops, and C. siamense has become increasingly prevalent worldwide. However, the mycelial growth and virulence of many of these species, especially below 26 °C, has largely remained unknown. Here, mycelial growth of five representative Colletotrichum strains (three C. siamense [Cs], one C. fructicola [Cf] and one C. gloeosporioides s.s. [Cg]) from diseased strawberry was measured at 10, 14, 18, 22 and 26 °C. Their virulence on Fragaria vesca and highly susceptible F. × ananassa cv. Benihoppe was tested at 14 °C and 22 °C. The strains did not differ much in mycelial growth between 10 and 26 °C, although growth of the Cs and Cg strains was somewhat faster than that of Cf. However, the strains varied greatly in their virulence on the two hosts. Strain Cs GQHZJ19, which grew fastest at 22 °C, was also the most virulent on both hosts at 14 °C and 22 °C. But growth rate of Cs was only positively correlated with virulence on susceptible cv. Benihoppe after 3 days at 22 °C. Based on the virulence analysis of the five Colletotrichum strains on hosts that vary in susceptibility at distinct temperatures, host genotype might play a decisive role in disease severity at an early stage, while temperature might influence the host–Colletotrichum spp. interaction at a later stage. More Colletotrichum strains (especially C. siamense) need to be tested to determine the mycelial growth and virulence in a wider temperature range and thus we can effectively manage disease.","PeriodicalId":15825,"journal":{"name":"Journal of General Plant Pathology","volume":"10 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141573888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Virulence analysis of wheat stem rust isolates (Puccinia graminis f. sp. tritici) in Iran using differential lines 利用差异株系对伊朗小麦茎锈病分离株（禾谷镰孢属三尖杉菌）进行毒力分析

IF 1.2 4区农林科学

Journal of General Plant Pathology Pub Date : 2024-07-05 DOI: 10.1007/s10327-024-01188-y

Fatemeh Khanboluki, Mahmoud Nasrollahi, Mansoor Karimi-Jashni

引用次数: 0

Colletotrichum species associated with loquat anthracnose in Kagawa and Tokushima prefectures, Japan 日本香川县和德岛县与枇杷炭疽病相关的 Colletotrichum 菌种

IF 1.2 4区农林科学

Journal of General Plant Pathology Pub Date : 2024-06-20 DOI: 10.1007/s10327-024-01185-1

Teeranai Poti, Gan Kisaki, Keishi Arita, Kazuya Akimitsu

{"title":"Colletotrichum species associated with loquat anthracnose in Kagawa and Tokushima prefectures, Japan","authors":"Teeranai Poti, Gan Kisaki, Keishi Arita, Kazuya Akimitsu","doi":"10.1007/s10327-024-01185-1","DOIUrl":"https://doi.org/10.1007/s10327-024-01185-1","url":null,"abstract":"Anthracnose caused by Colletotrichum species is a serious disease of loquat (Eriobotrya japonica), but the species of Colletotrichum that are associated with the disease in Japan have not been adequately surveyed. In this study, 46 isolates were isolated from diseased leaves and fruits of loquat in Kagawa and Tokushima prefectures in 2017 and 2018. On the basis of morphological features and molecular approaches, seven Colletotrichum species were identified: C. fioriniae, C. nymphaeae, C. karsti, C. aenigma, C. fructicola, C. gloeosporioides s. s., and C. siamense. The most prevalent species was C. karsti (43.5% of total species), followed by C. fioriniae (21.7%) and C. gloeosporioides s. s. (13.0%). All identified species were confirmed to cause loquat anthracnose by fulfilling Koch’s postulates. The loquat isolates of C. fioriniae, C. fructicola, and C. siamense also caused symptoms on all tested plants of kiwifruit, satsuma mandarin, persimmon, and peach. This report is the first on C. karsti and C. aenigma associated with loquat anthracnose and the first record of C. fructicola in Japan.","PeriodicalId":15825,"journal":{"name":"Journal of General Plant Pathology","volume":"182 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141547809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Converter slag causes gradual collapse of chlamydospores of the rice false smut fungus and reduces hyphal invasion into rice roots 转炉炉渣可使水稻假烟真菌的衣孢子逐渐溃散，并减少对水稻根部的菌丝侵染

IF 1.2 4区农林科学

Journal of General Plant Pathology Pub Date : 2024-06-01 DOI: 10.1007/s10327-024-01182-4

Kaichi Uchihashi, Takayuki Mitsunaga, Taketo Ashizawa

{"title":"Converter slag causes gradual collapse of chlamydospores of the rice false smut fungus and reduces hyphal invasion into rice roots","authors":"Kaichi Uchihashi, Takayuki Mitsunaga, Taketo Ashizawa","doi":"10.1007/s10327-024-01182-4","DOIUrl":"https://doi.org/10.1007/s10327-024-01182-4","url":null,"abstract":"Rice false smut has become a serious threat to rice production. Durable chlamydospores of the fungus are the primary inoculum in paddy soil. Mixing converter slag into paddy soil can decrease disease incidence, but the mechanism is unknown. Here we first confirmed the suppressive effect of converter slag mixed into a paddy field: 3t/ha suppressed disease incidence by 75.6%. We then tested a converter slag solution (CSS), neutralized CSS (NCSS), and converter-slag-amended soil extract (CSSE), all at 3t slag/ha. In the CSS test, chlamydospores rapidly became transparent, then gradually collapsed, but NCSS slowed chlamydospore collapse. More germ tubes were abnormal in the CSS treatment than in the NCSS. The percentages of transparent, collapsed, and normally germinating chlamydospores in the CSSE treatment were intermediate to those obtained with CSS and NCSS. Hyphal invasion into rice roots in CSSE was also suppressed compared to that in control. These results suggest chlamydospores gradually collapse when exposed to cations and the pH is neutralized by soil, thus reducing hyphal production and infection of rice roots, contributing to disease suppression in fields.","PeriodicalId":15825,"journal":{"name":"Journal of General Plant Pathology","volume":"32 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141194525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0