多态微卫星标记用于黑根腐病病原菌 Berkeleyomyces rouxiae 的遗传分析

IF 1 4区农林科学 Q3 PLANT SCIENCES

Journal of General Plant Pathology Pub Date : 2024-09-06 DOI:10.1007/s10327-024-01198-w

Akari Oya, Toshiyuki Usami

{"title":"多态微卫星标记用于黑根腐病病原菌 Berkeleyomyces rouxiae 的遗传分析","authors":"Akari Oya, Toshiyuki Usami","doi":"10.1007/s10327-024-01198-w","DOIUrl":null,"url":null,"abstract":"Berkeleyomyces rouxiae, a soil-borne fungal pathogen, causes black root rot on various plant species including carrot, okra, tobacco, lettuce, and other important crops. Here we analyzed the entire genome sequence of a Japanese isolate of B. rouxiae and discovered 25 microsatellite sequences. These sequences were amplified from the genomic DNA of several Japanese isolates by PCR and sequenced, revealing that the microsatellite sequences were polymorphic among isolates. These markers are useful for phylogenetic analysis, epidemiological population analysis, identification of specific isolates, and other genetic investigation of B. rouxiae.","PeriodicalId":15825,"journal":{"name":"Journal of General Plant Pathology","volume":"80 1","pages":""},"PeriodicalIF":1.0000,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Polymorphic microsatellite markers for genetic analysis of Berkeleyomyces rouxiae, a causal agent of black root rot\",\"authors\":\"Akari Oya, Toshiyuki Usami\",\"doi\":\"10.1007/s10327-024-01198-w\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Berkeleyomyces rouxiae, a soil-borne fungal pathogen, causes black root rot on various plant species including carrot, okra, tobacco, lettuce, and other important crops. Here we analyzed the entire genome sequence of a Japanese isolate of B. rouxiae and discovered 25 microsatellite sequences. These sequences were amplified from the genomic DNA of several Japanese isolates by PCR and sequenced, revealing that the microsatellite sequences were polymorphic among isolates. These markers are useful for phylogenetic analysis, epidemiological population analysis, identification of specific isolates, and other genetic investigation of B. rouxiae.\",\"PeriodicalId\":15825,\"journal\":{\"name\":\"Journal of General Plant Pathology\",\"volume\":\"80 1\",\"pages\":\"\"},\"PeriodicalIF\":1.0000,\"publicationDate\":\"2024-09-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of General Plant Pathology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1007/s10327-024-01198-w\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of General Plant Pathology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s10327-024-01198-w","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PLANT SCIENCES","Score":null,"Total":0}

引用次数: 0

摘要

胭脂虫（Berkeleyomyces rouxiae）是一种土传真菌病原体，会导致胡萝卜、秋葵、烟草、莴苣等多种重要作物的黑根腐病。在这里，我们分析了日本分离的胭脂虫的整个基因组序列，发现了 25 个微卫星序列。通过 PCR 从几个日本分离株的基因组 DNA 中扩增出这些序列并进行测序，发现这些微卫星序列在不同分离株之间具有多态性。这些标记有助于对胭脂虫进行系统发育分析、流行病学种群分析、特定分离株的鉴定以及其他遗传学研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Polymorphic microsatellite markers for genetic analysis of Berkeleyomyces rouxiae, a causal agent of black root rot

查看原文本刊更多论文

Polymorphic microsatellite markers for genetic analysis of Berkeleyomyces rouxiae, a causal agent of black root rot

Berkeleyomyces rouxiae, a soil-borne fungal pathogen, causes black root rot on various plant species including carrot, okra, tobacco, lettuce, and other important crops. Here we analyzed the entire genome sequence of a Japanese isolate of B. rouxiae and discovered 25 microsatellite sequences. These sequences were amplified from the genomic DNA of several Japanese isolates by PCR and sequenced, revealing that the microsatellite sequences were polymorphic among isolates. These markers are useful for phylogenetic analysis, epidemiological population analysis, identification of specific isolates, and other genetic investigation of B. rouxiae.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Journal of General Plant Pathology PLANT SCIENCES-

CiteScore

2.40

自引率

8.30%

发文量

审稿时长

6 months

期刊介绍： The Journal of General Plant Pathology welcomes all manuscripts dealing with plant diseases or their control, including pathogen characterization, identification of pathogens, disease physiology and biochemistry, molecular biology, morphology and ultrastructure, genetics, disease transmission, ecology and epidemiology, chemical and biological control, disease assessment, and other topics relevant to plant pathological disorders.